CN109298126B - Panel paving device and panel paving method of thin-layer chromatographic plate - Google Patents
Panel paving device and panel paving method of thin-layer chromatographic plate Download PDFInfo
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- CN109298126B CN109298126B CN201811360716.7A CN201811360716A CN109298126B CN 109298126 B CN109298126 B CN 109298126B CN 201811360716 A CN201811360716 A CN 201811360716A CN 109298126 B CN109298126 B CN 109298126B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/92—Construction of the plate
- G01N30/93—Application of the sorbent layer
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Abstract
The invention discloses a plate paving device of a thin-layer chromatographic plate, which comprises a material box, wherein a film pump is arranged in the material box, one side of the material box is connected with a hose, the other end of the hose is connected with a plate paving mechanism assembly, the film pump is connected with a system controller through a relay, and the system controller is also connected with a vacuum pump and a three-degree-of-freedom manipulator respectively. The plate paving device of the thin-layer chromatographic plate has the advantages of small equipment volume, high efficiency and strong practicability; when the thin-layer chromatography plate is prepared, no solid adsorbs dust, no air bubbles exist in the adsorbent homogenate, and the paved thin-layer plate has good uniformity.
Description
Technical Field
The invention belongs to the technical field of thin-layer chromatography instruments and equipment, particularly relates to a thin-layer chromatography plate paving device, and further relates to a paving method of the thin-layer chromatography plate paving device.
Background
Thin-layer chromatography is a chromatographic separation technique in which a support coated on a glass plate is used as a stationary phase, and a suitable solvent is used as a mobile phase to separate, identify and quantify a mixed sample. The thin-layer chromatography plate is a main tool for thin-layer chromatography separation, and the quality of the plate directly determines the separation effect. At present, the thin-layer chromatography plate is mostly prepared manually, and the main process is as follows: grinding and mixing the adsorbent with water or aqueous solution added with adhesive in a mortar to obtain adsorbent homogenate, and pouring onto a glass plate to form a thin layer; in order to ensure that the thickness of the coating of the adsorbent homogenate on the glass plate is uniform, the thin-layer plate is usually required to be overturned on a test bed for many times; or a thin layer is prepared with an applicator, but the speed at which the applicator is moved affects the thickness of the thin layer. In practice, the thin-layer plate often has grooves, lines, roughness, pocks, bubbles, unevenness or lumps in the coating. Therefore, it is difficult to perform an effective separation experiment, which seriously affects the separation effect.
Disclosure of Invention
The invention aims to provide a plate paving device of a thin-layer chromatographic plate, which solves the problems of uneven thickness of a coating and more bubbles in manual preparation of the thin-layer chromatographic plate in the prior art.
The technical scheme includes that the plate paving device of the thin-layer chromatographic plate comprises a material box, a thin film pump is arranged in the material box, one side of the material box is connected with a hose, the other end of the hose is connected with a plate paving mechanism assembly, the thin film pump is connected with a system controller through a relay, and the system controller is respectively connected with a vacuum pump three-degree-of-freedom manipulator.
The invention is also characterized in that:
the plate paving mechanism assembly comprises a box body a, the bottom of the box body a is empty, a clamping rod a is arranged on one side face of the box body a close to the top, at least one locking hole a is arranged on each side face of the box body a, a spring push rod penetrates through the locking hole a, a motor is arranged at the center of the top of the box body a, the motor is connected with the top of the box body a through a flange, a synchronous cam is sleeved on an axis of the motor, the spring push rod is in contact with the synchronous cam, a rolling driving rod is arranged at the diagonal line of the inner side of the top of the box body a, and the rolling driving.
The plate paving mechanism assembly further comprises a box body b, the top of the box body b is empty, the box body b is placed on the bottom plate, protrusions are arranged on the periphery of the bottom plate, a material return groove is formed between the protrusions of the bottom plate and the side face of the box body b, a clamping rod b is arranged on one side face of the box body b, a locking hole b corresponding to the locking hole a on the side face of the box body a is arranged on each side face of the box body b, the box body a is sleeved in the box body b, a feeding hole is formed in the other side face of the box body b, the feeding hole is connected with a hose, and a discharging hole is further formed.
The spring push rod comprises a lock supporting rod, the lock supporting rod is matched with the lock catch hole a, a movable rod is arranged at one end of the lock supporting rod, a spring is sleeved on the movable rod, the lock supporting rod and the movable rod are coaxial, and the spring push rod is perpendicular to the side face, corresponding to the lock catch hole a, of the lock catch hole a.
The rolling deflector rod comprises a deflector rod, a deflector rod rotating shaft is arranged in the axial direction perpendicular to the deflector rod, the deflector rod is rotatably connected with the deflector rod rotating shaft, the deflector rod is in a flat rod shape, the radian of two ends of the deflector rod is different, a spring with a spring pressing ball is arranged at one end, with a large radian, of the deflector rod and close to one side of the box body a, a blind hole is formed in the contact position of the deflector rod and the spring, a mechanical ring is arranged at the bottom of the blind hole and fixedly connected with the spring pressing ball, and the spring pressing direction of the spring pressing ball is.
The inner side of the top of the box body a is also provided with at least two vacuum chucks, each vacuum chuck is hermetically connected with a vacuum pump through threads, and the vacuum chucks are used for adsorbing the thin-layer chromatography plate.
The vacuum chuck, the rolling deflector rod and the spring push rod are not contacted with each other.
The synchronous cam is clover-shaped.
The invention adopts another technical scheme that the plate paving method of the plate paving device of the thin-layer chromatographic plate is implemented according to the following steps:
step 1: starting a power supply, setting feeding time and feeding quantity on a system controller, filling materials into a material box, connecting a hose to a feeding hole in the box body b, starting a film pump, and spraying the materials into the box body b through the hose;
step 2: the system controller starts a vacuum pump, and controls the three-degree-of-freedom manipulator to place the thin-layer chromatographic plate at the vacuum chuck, so that the thin-layer chromatographic plate just covers the bottom of the box body a, and meanwhile, the deflector rod rotates under the driving of the synchronous cam to press the spring pressing ball into the spring;
and step 3: the system controller controls the three-degree-of-freedom manipulator to slowly press the box body a into the box body b, and excessive materials in the box body b are discharged from the discharge port to the material return groove along with the downward pressing of the box body a and are recovered;
and 4, step 4: stopping pressing when the distance between the thin-layer chromatography plate and the bottom of the box body b is 2-4 mm, starting a motor by a system controller, controlling a synchronous cam to rotate to enable a spring push rod to eject a lock supporting rod, and fixing the box body a and the box body b;
and 5: after the system controller controls the two three-degree-of-freedom manipulators to turn the box body a and the box body b for 180 degrees, the two three-degree-of-freedom manipulators move and separate relatively, and meanwhile, the motor 7 controls the synchronous cam to rotate to enable the spring push rod to retract into the lock supporting rod, so that the box body a and the box body b are separated;
step 6: and the system controller controls the three-degree-of-freedom manipulator to move the box body b away from the box body a, meanwhile, the vacuum pump is turned off, the vacuum chuck is deflated, the motor 7 controls the synchronous cam to rotate, so that the rolling deflector rod drives the spring pressing ball to ascend and eject out the thin-layer chromatographic plate, and the prepared thin-layer chromatographic plate is taken out.
The invention has the beneficial effects that:
the plate paving device of the thin-layer chromatographic plate has the advantages of small equipment volume, high efficiency and strong practicability; when the thin-layer chromatography plate is prepared, no solid adsorbent dust exists, no air bubbles exist in the adsorbent homogenate, and the paved thin-layer plate has good uniformity; the plate paving device of the thin-layer chromatographic plate realizes plate paving integration and intellectualization and ensures quality stability.
Drawings
FIG. 1 is a schematic view of the structure of a plate-laying apparatus of the thin layer chromatography plate of the present invention;
FIG. 2 is a schematic structural view of a case a of the present invention;
fig. 3 is a schematic structural view of the case b of the present invention.
In the figure, 1, a material box, 2, a clamping rod a, 3, a hose, 4, a box a, 5, a box b, 6, a three-degree-of-freedom manipulator, 7, a motor, 8, a clamping rod b, 9, a feeding hole, 10, a discharging hole, 11, a material returning groove, 12, a vacuum chuck, 13, a locking hole a, 14, a locking hole b, 15, a plate paving mechanism assembly, 16, a spring push rod, 16-1, a locking support rod, 16-2, a movable rod, 16-3, a spring, 17, a synchronous cam, 18, a rolling driving rod, 18-1, a driving rod, 18-2, a driving rod rotating shaft and 18-3, a spring pressing ball are arranged.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
The invention discloses a plate paving device of a thin-layer chromatography plate, which comprises a material box 1, wherein a film pump is arranged in the material box 1, one side of the material box 1 is connected with a hose 3, the other end of the hose 3 is connected with a plate paving mechanism assembly 15, the film pump is connected with a system controller through a relay, and the system controller is respectively connected with a vacuum pump and a three-degree-of-freedom manipulator 6.
Further:
as shown in fig. 2, the plate paving mechanism assembly 15 includes a box a4, the bottom of the box a4 is hollow, a clamping rod a2 is disposed at a position, close to the top, of one side surface of the box a4, at least one locking hole a13 is disposed at each side surface of the box a4, a spring push rod 16 penetrates through each locking hole a13, a motor 7 is disposed at the center of the top of the box a4, the motor 7 is in flange connection with the top of the box a4, a synchronizing cam 17 is sleeved on a shaft of the motor 7, the spring push rod 16 is in contact with the synchronizing cam 17, rolling shift levers 18 are disposed at diagonal positions inside the top of the box a4, and the rolling shift levers 18 are in contact with the synchronizing cam 17.
Further:
as shown in fig. 3, the plate spreading mechanism assembly 15 further includes a box b5, the top of the box b5 is hollow, the box b5 is placed on the bottom plate, the periphery of the bottom plate is provided with protrusions, a material returning groove 11 is formed between the protrusions of the bottom plate and the side surface of the box b5, one side surface of the box b5 is provided with a clamping rod b8, each side surface of the box b5 is provided with a locking hole b14 corresponding to the locking hole a13 on the side surface of the box a4, the box a4 is sleeved in the box b5, the other side surface of the box b5 is provided with a feeding port 9, the feeding port 9 is connected with the hose 3, and each side surface of the box b5 is further provided with a discharging port 10.
Further:
the spring push rod 16 comprises a lock support rod 16-1, the lock support rod 16-1 is matched with the lock catch hole a13, one end of the lock support rod 16-1 is provided with a movable rod 16-2, the movable rod 16-2 is sleeved with a spring 16-3, and the lock support rod 16-1 and the movable rod 16-2 are coaxial and perpendicular to the corresponding side surface provided with the lock catch hole a 13.
Further:
the rolling deflector rod 18 comprises a deflector rod 18-1, a deflector rod rotating shaft 18-2 is arranged in the axial direction perpendicular to the deflector rod 18-1, the deflector rod 18-1 is rotatably connected with the deflector rod rotating shaft 18-2, the deflector rod 18-1 is in a flat rod shape, the radians of two ends of the deflector rod 18-1 are different, a spring with a spring pressing ball 18-3 is arranged at one end with a large radian of the deflector rod 18-1 and close to one side of the box body a4, a blind hole is arranged at the contact position of the deflector rod 18-1 and the spring, a mechanical ring is arranged at the bottom of the blind hole and fixedly connected with the spring pressing ball 18-3, and the elastic pressing direction of the spring pressing ball 18-3 is perpendicular to.
Further:
the inner side of the top of the box body a4 is also provided with at least two vacuum chucks 12, each vacuum chuck 12 is connected with a vacuum pump in a sealing way through a screw thread, and the vacuum chucks 12 are used for adsorbing the thin-layer chromatography plate.
Further:
the vacuum cup 12, the rolling shift lever 18 and the spring push rod 16 are not in contact with each other.
Further:
the synchronizing cam 17 is clover-shaped.
The invention provides a preferable scheme of a plate paving device of a thin layer chromatography plate, which comprises the following steps:
the invention relates to a plate paving device of a thin-layer chromatographic plate, which comprises a material box 1, wherein a film pump is arranged in the material box 1, one side of the material box 1 is connected with a hose 3, the other end of the hose 3 is connected with a plate paving mechanism assembly 15, the film pump is connected with a system controller through a relay, and the system controller is also respectively connected with a vacuum pump and a three-degree-of-freedom manipulator 6; as shown in fig. 2, the plate paving mechanism assembly 15 includes a box a4, the bottom of the box a4 is hollow, a clamping rod a2 is disposed at a position close to the top of one side of the box a4, each side of the box a4 is provided with at least one locking hole a13, a spring push rod 16 penetrates through the locking hole a13, the spring push rod 16 includes a locking support rod 16-1, the locking support rod 16-1 is matched with the locking hole a13, one end of the locking support rod 16-1 is provided with a movable rod 16-2, the movable rod 16-2 is sleeved with a spring 16-3, the locking support rod 16-1 and the movable rod 16-2 are coaxial and perpendicular to the corresponding side provided with the locking hole a13, the top center of the box a4 is provided with a motor 7, the motor 7 is connected with a flange at the top of the box a4, a synchronizing cam 17 is sleeved on a shaft of the motor 7, the synchronizing cam 17 is in a clover shape, the spring push rod, the diagonal lines on the inner side of the top of the box body a4 are respectively provided with a rolling deflector rod 18, the rolling deflector rod 18 is in contact with a synchronous cam 17, the rolling deflector rod 18 comprises a deflector rod 18-1, a deflector rod rotating shaft 18-2 is arranged in a direction perpendicular to the axial direction of the deflector rod 18-1, the deflector rod 18-1 is in rotary connection with the deflector rod rotating shaft 18-2, the deflector rod 18-1 is in a flat rod shape, the radians of two ends of the deflector rod 18-1 are different, the end with the large radian of the deflector rod 18-1 is provided with a spring pressing ball 18-3 at one side close to the box body a4, a blind hole is arranged at the contact part of the deflector rod 18-1 and the spring, the bottom of the blind hole is provided with a mechanical ring, the mechanical ring is fixedly connected with the spring; the inner side of the top of the box body a4 is also provided with at least two vacuum chucks 12, each vacuum chuck 12 is hermetically connected with a vacuum pump through a thread, and the vacuum chucks 12 are used for adsorbing thin-layer chromatography plates; the vacuum chuck 12, the rolling deflector rod 18 and the spring push rod 16 are not contacted with each other; the plate spreading mechanism assembly 15 further comprises a box body b5, the top of the box body b5 is hollow, the box body b5 is placed on the bottom plate, protrusions are arranged on the periphery of the bottom plate, a material returning groove 11 is formed between the protrusions of the bottom plate and the side face of the box body b5, a clamping rod b8 is arranged on one side face of the box body b5, each side face of the box body b5 is provided with a locking hole b14 corresponding to the locking hole a13 on the side face of the box body a4, the box body a4 is sleeved in the box body b5, the other side face of the box body b5 is provided with a feeding hole 9, the feeding hole 9 is connected with the hose 3, and each side face of the box body b 5.
The invention also provides a plate paving method of the plate paving device of the thin-layer chromatographic plate, which is implemented according to the following steps:
step 1: starting a power supply, setting feeding time and feeding quantity on a system controller, filling materials (namely adsorbent homogenate) into a material box 1, connecting a hose 3 to a feeding hole 9 on a box body b5, starting a membrane pump, and spraying the materials into a box body b5 through the hose 3;
step 2: the system controller starts a vacuum pump, controls the three-degree-of-freedom manipulator 6 to place the thin-layer chromatographic plate on the vacuum chuck 12, enables the thin-layer chromatographic plate to just cover the bottom of the box body a4, and simultaneously drives the deflector rod 18-1 to rotate under the driving of the synchronous cam 17 to press the spring pressing ball 18-3 into the spring;
and step 3: the system controller controls the three-degree-of-freedom manipulator 6 to slowly press the box body a4 into the box body b5, and excessive materials in the box body b5 are discharged from the discharge port 10 to the feed back trough 11 along with the downward pressing of the box body a4 and are recovered;
and 4, step 4: stopping pressing when the thin-layer chromatography plate is 2-4 mm away from the bottom of the box body b5, starting the motor 7 by the system controller, controlling the synchronous cam 17 to rotate to enable the spring push rod 16 to eject the lock support rod 16-1, and fixing the box body a4 and the box body b 5;
and 5: after the system controller controls the two three-degree-of-freedom manipulators 6 to turn the box body a4 and the box body b5 for 180 degrees, the two three-degree-of-freedom manipulators 6 move and separate relatively, and meanwhile, the motor 7 controls the synchronous cam 17 to rotate to enable the spring push rod 16 to retract into the lock support rod 16-1, so that the box body a4 and the box body b5 are separated;
step 6: and the system controller controls the three-degree-of-freedom manipulator 6 to move the box b5 away from the box a4, simultaneously, the vacuum pump is closed, the vacuum chuck 12 is deflated, the motor 7 controls the synchronous cam 17 to rotate, so that the rolling deflector rod 18 drives the spring pressing ball 18-3 to ascend and eject the thin-layer chromatographic plate, and the prepared thin-layer chromatographic plate is taken out.
The plate paving device of the thin-layer chromatographic plate has the advantages that:
(1) the plate paving device of the thin-layer chromatographic plate has the advantages of small equipment volume, high efficiency and strong practicability;
(2) according to the plate paving device for the thin-layer chromatography plate, when the thin-layer chromatography plate is prepared, no solid adsorbent dust exists, no air bubbles exist in the adsorbent homogenate, and the paved thin-layer plate has good uniformity;
(3) the plate paving device of the thin-layer chromatographic plate realizes plate paving integration and intellectualization, ensures quality stability and also reduces working strength.
Claims (6)
1. The plate paving device of the thin-layer chromatographic plate is characterized by comprising a material box (1), wherein a film pump is arranged in the material box (1), one side of the material box (1) is connected with a hose (3), the other end of the hose (3) is connected with a plate paving mechanism assembly (15), the film pump is connected with a system controller through a relay, and the system controller is also respectively connected with a vacuum pump and a three-degree-of-freedom manipulator (6);
the plate paving mechanism assembly (15) comprises a box body a (4), the bottom of the box body a (4) is hollow, a clamping rod a (2) is arranged at a position, close to the top, of one side face of the box body a (4), each side face of the box body a (4) is provided with at least one locking hole a (13), a spring push rod (16) penetrates through each locking hole a (13), a motor (7) is arranged at the center of the top of the box body a (4), the motor (7) is in flange connection with the top of the box body a (4), a synchronous cam (17) is sleeved on a shaft of the motor (7), the spring push rod (16) is in contact with the synchronous cam (17), rolling deflector rods (18) are respectively arranged at diagonal positions of the inner side of the top of the box body a (4), and the rolling deflector rods (18) are in contact with the synchronous cam (17);
the paving plate mechanism assembly (15) further comprises a box body b (5), the top of the box body b (5) is hollow, the box body b (5) is placed on the bottom plate, bulges are arranged on the periphery of the bottom plate, a material return groove (11) is formed between the bulges of the bottom plate and the side surface of the box body b (5), a clamping rod b (8) is arranged on one side surface of the box body b (5), a locking hole b (14) corresponding to a locking hole a (13) on the side surface of the box body a (4) is arranged on each side surface of the box body b (5), the box body a (4) is sleeved in the box body b (5), a feeding hole (9) is arranged on the other side surface of the box body b (5), the feeding hole (9) is connected with the hose (3), and a discharging hole (10) is further arranged on each side surface of the box body b (5);
the top inboard of box a (4) still is provided with two at least vacuum chuck (12), every vacuum chuck (12) are through screw and vacuum pump sealing connection, vacuum chuck (12) are used for adsorbing the thin layer chromatography board.
2. The plate paving device of the thin layer chromatography plate as claimed in claim 1, wherein the spring push rod (16) comprises a lock support rod (16-1), the lock support rod (16-1) is matched with the lock catch hole a (13), a movable rod (16-2) is arranged at one end of the lock support rod (16-1), a spring (16-3) is sleeved on the movable rod (16-2), and the lock support rod (16-1) and the movable rod (16-2) are coaxial and perpendicular to the corresponding side surface provided with the lock catch hole a (13).
3. The device for laying sheets of TLC plates according to claim 2, characterized in that the rolling deflector (18) comprises a deflector (18-1), a deflector pivot axis (18-2) is arranged perpendicular to the axial direction of the deflector (18-1), the deflector rod (18-1) is rotationally connected with the deflector rod rotating shaft (18-2), the deflector rod (18-1) is in a flat rod shape, the radian of the two ends of the deflector rod (18-1) is different, the radian of the end of the deflector rod (18-1) is larger, and one side close to the box body a (4) is provided with a spring pressing ball (18-3), a blind hole is arranged at the contact position of the deflector rod (18-1) and the spring, a mechanical ring is arranged at the bottom of the blind hole, the mechanical ring is fixedly connected with a spring pressing ball (18-3), and the elastic pressing direction of the spring pressing ball (18-3) is vertical to the top of the box body a (4).
4. The device for laying sheets of TLC plates as claimed in claim 3, characterized in that the vacuum cup (12), the roller plunger (18) and the spring plunger (16) do not touch each other.
5. The device for laying sheets of TLC as claimed in claim 4, characterized in that the synchronizing cam (17) is clover-shaped.
6. A method of laying a sheet of a laying device for thin layer chromatography plates according to claim 5, comprising the steps of:
step 1: starting a power supply, setting feeding time and feeding quantity on a system controller, filling materials into a material box (1), connecting a hose (3) to a feeding hole (9) on a box body b (5), starting a film pump, and spraying the materials into the box body b (5) through the hose (3);
step 2: the system controller starts a vacuum pump, and controls the three-degree-of-freedom manipulator (6) to place the thin-layer chromatographic plate on the vacuum sucker (12) so that the thin-layer chromatographic plate just covers the bottom of the box body a (4), and meanwhile, the deflector rod (18-1) rotates under the driving of the synchronous cam (17) to press the spring pressing ball (18-3) into the spring;
and step 3: the system controller controls the three-degree-of-freedom manipulator (6) to slowly press the box body a (4) into the box body b (5), and excessive materials in the box body b (5) are discharged from the discharge hole (10) to the material return groove (11) along with the downward pressing of the box body a (4) and are recovered;
and 4, step 4: stopping pressing when the thin-layer chromatography plate is 2-4 mm away from the bottom of the box body b (5), starting a motor (7) by a system controller, controlling a synchronous cam (17) to rotate to enable a spring push rod (16) to eject out a lock supporting rod (16-1), and fixing the box body a (4) and the box body b (5);
and 5: after the system controller controls the two three-degree-of-freedom mechanical arms (6) to turn the box body a (4) and the box body b (5) for 180 degrees, the two three-degree-of-freedom mechanical arms (6) move and separate relatively, and meanwhile, the motor (7) controls the synchronous cam (17) to rotate to enable the spring push rod (16) to retract into the lock support rod (16-1), so that the box body a (4) and the box body b (5) are separated;
step 6: and the system controller controls the three-degree-of-freedom manipulator (6) to move the box body b (5) and the box body a (4) away, simultaneously, the vacuum pump is closed, the vacuum sucker (12) is deflated, the motor (7) controls the synchronous cam (17) to rotate, so that the rolling deflector rod (18) drives the spring pressing ball (18-3) to ascend and eject the thin-layer chromatographic plate, and the prepared thin-layer chromatographic plate is taken out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811360716.7A CN109298126B (en) | 2018-11-15 | 2018-11-15 | Panel paving device and panel paving method of thin-layer chromatographic plate |
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| CN201811360716.7A CN109298126B (en) | 2018-11-15 | 2018-11-15 | Panel paving device and panel paving method of thin-layer chromatographic plate |
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| CN109298126A CN109298126A (en) | 2019-02-01 |
| CN109298126B true CN109298126B (en) | 2020-08-25 |
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| CN107931004A (en) * | 2017-12-15 | 2018-04-20 | 佛山市万良商贸有限公司 | A kind of device for painting for paperboard strip processing |
| CN207798759U (en) * | 2018-02-07 | 2018-08-31 | 山西大同大学 | A kind of high efficiency chromatosheet device for board manufacture |
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