CN109297791A - A kind of migration cell and its application method - Google Patents
A kind of migration cell and its application method Download PDFInfo
- Publication number
- CN109297791A CN109297791A CN201811287116.2A CN201811287116A CN109297791A CN 109297791 A CN109297791 A CN 109297791A CN 201811287116 A CN201811287116 A CN 201811287116A CN 109297791 A CN109297791 A CN 109297791A
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- fermentation vat
- migration cell
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- 238000013508 migration Methods 0.000 title claims abstract description 43
- 230000005012 migration Effects 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 66
- 238000000855 fermentation Methods 0.000 claims abstract description 45
- 230000004151 fermentation Effects 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 21
- 230000008676 import Effects 0.000 claims description 4
- 238000010992 reflux Methods 0.000 abstract description 12
- 238000007654 immersion Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 description 13
- 239000011521 glass Substances 0.000 description 7
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 5
- 229910052731 fluorine Inorganic materials 0.000 description 5
- 239000011737 fluorine Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000008236 heating water Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of migration cell and its application methods, migration cell includes pad lid, U-shaped fermentation vat, impregnates locked instrument, the fermentation vat includes that sample arm, liquid adding arm, both ends are separately connected the pond body of sample arm lower end and liquid adding arm lower end, and pad cover buckle is covered in sample arm upper end opening and fixed by immersion locked instrument.Convenient for accurately controlling S/V, and disturbing factor is few, is suitble to condensing reflux, is vdiverse in function, easy to operate.
Description
Technical field
The invention belongs to experimental instrument technology, it is related to a kind of migration cell and its application method.
Background technique
Packaging material is widely used to food packaging industry, is provided according to standard, for food contact material and product
It needs to carry out the amount of migration test, and meets corresponding standard requirements.Such as GB 31604.1-2015 " food contact material and system
Product migration test general rule " standard regulation is when carrying out migration test, the contact area (S) of food contact material and product and food mould
The ratio (S/V) of quasi- object product or quality (V) reflects actual use situation；When that can not estimate the value, 6dm is generally used2Food
The food or food simulants of product contact material and product into contact 1kg.In standard GB 5009.156-2016 " food contact material
And product migration test preprocess method general rule " in give the example of novel migration cell, and be illustrated.For certain spies
Establish rules lattice, shape material for, tested without using novel migration cell is then more difficult.
Patent CN201622227U proposes a kind of food contact material analogies migration test pond, the test tank main body
Including accommodating pond, sealing ring, pedestal and locked instrument, the accommodating pond band sealing ring.Since sealing ring is in accommodating pond one
Side, in test, analogies by direct Contact Seal ring, move out by the substance inevitably resulted in sealing ring, and sealing ring is deposited
In the accumulation for also be easy to causeing the pollutants such as dust, the cleaning difficulty in accommodating pond is improved.In addition, for the operation that needs flow back,
Generally require carry out heating water bath, and current migration cell, soak is located at the top of sample, and when heating water bath cannot directly add
Heat arrives soak.
Summary of the invention
In order to solve the problems in the prior art, the purpose of the present invention is to propose a kind of migration cell and its application method, just
In accurately control S/V, and disturbing factor is few, is suitble to condensing reflux, is vdiverse in function, easy to operate.
Object of the invention can be realized by the following technical scheme: a kind of migration cell, which is characterized in that covered including pad,
U-shaped fermentation vat impregnates locked instrument, and the fermentation vat includes that sample arm, liquid adding arm, both ends are separately connected under sample arm
The pond body at end and liquid adding arm lower end, pad cover buckle are covered in sample arm upper end opening and are fixed by impregnating locked instrument.
Simple scenario, a kind of migration cell, which is characterized in that it is made of pad lid, U-shaped fermentation vat, immersion locked instrument,
The fermentation vat includes that sample arm, liquid adding arm, both ends are separately connected the pond body of sample arm lower end and liquid adding arm lower end, pads cover buckle
It covers in sample arm upper end opening and is fixed by impregnating locked instrument.
Preferably, the sample arm upper end opening has the annular protrusive board to extend horizontally outward.Sample to be tested is divided
It is placed on annular protrusive board, is then covered again with backing plate above.
Preferably, the fermentation vat locked instrument is one in spiral locking, buckle-type locking or circle hoop formula locking
Kind.The fermentation vat locked instrument can know that such as circle hoop formula locking can be used similar by the prior art
Adjustable fixator in CN206382027U, therefore be not further described herein.
Preferably, the liquid adding arm height is greater than sample arm height.When test, the soak poured into is full of liquid adding arm,
Height in liquid adding arm is greater than the height in sample arm.Since most soak is in the lower section of sample, so by impregnating
The pressure that liquid generates is smaller, reduces the risk that soak is leaked out from sample edge.
Preferably, when soak liquid volume added reaches sample arm height in fermentation vat, at sample arm sample exposed area with
The ratio between fermentation vat liquid volume added is not less than 0.1cm2/mL.Preferably, when in fermentation vat soak liquid volume added reach sample arm height
When, the ratio between sample exposed area and fermentation vat liquid volume added are not less than 0.3cm at sample arm2/mL.Preferably, when being soaked in fermentation vat
When bubble liquid liquid volume added reaches sample arm height, the ratio between sample exposed area and fermentation vat liquid volume added are not less than at sample arm
0.5cm2/mL.Preferably, when soak liquid volume added reaches sample arm height in fermentation vat, sample exposed area at sample arm
It is 0.6cm with the ratio between fermentation vat liquid volume added2/mL。
When sample exposed area refers to that sample is in sample arm at sample arm described in this patent, sample does not have and sample
Product arm contact, it is contemplated that and the area of soak contact.
Preferably, the volume of the fermentation vat is greater than 200mL, and works as soak liquid volume added in fermentation vat and reach sample
When arm height, liquid volume added is less than 200mL.When i.e. the soak of 200mL is added in fermentation vat, immersion is filled in sample arm
Liquid, and the height of soak is higher than the height in sample arm in liquid adding arm, and then ensures that soak comes into full contact with sample.
Preferably, the volume of the fermentation vat is 250mL~350mL.
Preferably, sample exposed area is not less than 0.25dm at the sample arm2.It is further preferred that the sample arm
Place's sample exposed area is 1.2dm2。
Preferably, the fermentation vat is made of glass.Preferably, the pad lid, fermentation vat are made of glass.
Preferably, the pad, which covers, is provided with seal groove, is provided with sealing ring in seal groove.In use, sample
Test surfaces are placed towards sample arm, and the pad lid equipped with sealing ring one side covers on sample.The setting of sealing ring, can reduce analogies
Risk of leakage during the test；It contacts, reduces in test process with sealing ring moreover, analogies are not direct in test process
Disturbing factor.
Preferably, the sealing ring is made of silicon rubber or fluorine material.Fluorine material can reduce food to greatest extent
The influence that analogies may cause test result, effect are more preferable.
Preferably, sample exposed area is not less than 0.25dm within the seal groove2.It is further preferred that described
Sample exposed area is 1.2dm within seal groove2.Sample exposed area is thumb pad within seal groove described in this patent
The face being bonded with sample arm/sample is covered, to start at the half of seal groove width, calculates the area in seal groove.Such as pad
The face being bonded with sample arm/sample is covered, the shape of seal groove is annulus, and interior diameter of a circle is 0.8dm, and outer diameter of a circle is
1dm then takes diameter for 0.9dm calculating when calculating sample exposed area within seal groove.
Preferably, the pond is as flat.Migration cell can be placed in the horizontal plane by the flat stabilization of pond body.
Preferably, the sample arm or liquid adding arm are cased with outside is fixedly connected with block.When carrying out heating water bath, due to the boiling of water
Rise generation active force, migration cell may be made to topple over, therefore can be connected by being fixedly connected with block with fixed device, to migration cell into
Row is further fixed.
Preferably, the migration cell further includes liquid adding arm adapting device, the upper end of liquid adding arm adapting device and liquid adding arm
Import cooperation.Liquid adding arm adapting device primarily serves the effect of sealing, condensing reflux etc..In the simplest case, plastics are thin
Film can also be used as liquid adding arm adapting device winding cooperation in the upper end import of liquid adding arm.Preferably, liquid adding arm cooperation
Device is Chi Gai or condensing reflux pipe.When needing to be condensed back, Jiang Chigai replaces with condensing reflux pipe, and condensing reflux pipe makes to soak
Bubble liquid steam cooling flows back in liquid adding arm again.Preferably, Chi Gai is made of glass or fluorine material.Preferably, condensing reflux pipe
For glass condensing reflux pipe.
The invention also discloses a kind of application methods of above-mentioned migration cell, which is characterized in that comprises the following steps:
(a) sample is placed in sample arm, sample test faces sample arm, covers pad lid, uses immersion locked instrument
By pad lid and fermentation vat locking；
(b) soak is added by liquid adding arm；
(c) it is tested.
When necessary, bleeding is carried out after soak is added, contacts sample with soak better.In general, row
Only migration cell need to suitably be tilted toward sample arm side when gas, exclude remaining air in sample arm.
Compared with prior art, this migration cell has the advantage that
1, sealing ring is arranged on the outside of sample, reduces disturbing factor in test process, especially grease type analogies and tests
Under the conditions of, sealing ring is such as immersed directly in soak, then influencing on experimental result will be very big.
2, the migration cell of this patent design can preferably carry out blank assay.
3, the migration cell of this patent design can be suitable for the heating of the immersions such as water-bath, oil bath and to test sample without shadow
It rings.
4, using the fermentation vat of similar "u"-shaped, the pressure that soak generates sample can be effectively reduced, to drop
The risk of low soak leakage.
5, the migration cell of this patent design can realize the operation such as condensing reflux, fluid infusion by liquid adding arm adapting device.
It should be noted that fermentation vat U-shaped described in this patent is not proper U-typed, such as impregnate
There may be the gradual changes or mutation in size for the structures such as sample arm, liquid adding arm, the pond body in pond.
In case of no particular description, soak described in this patent and analogies can be equivalent.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of this migration cell.
In figure, 1, pad lid；11, seal groove；2, fermentation vat；21, liquid adding arm；22, sample arm；23, pond body；24, annular
Protrusive board；3, locked instrument is impregnated；4, liquid adding arm adapting device；5, it is fixedly connected with block；6, sealing ring.
Specific embodiment
Following is a specific embodiment of the present invention in conjunction with the accompanying drawings, technical scheme of the present invention will be further described,
However, the present invention is not limited to these examples.
As shown in Figure 1, this migration cell includes pad lid 1, U-shaped fermentation vat 2, impregnates locked instrument 3, liquid adding arm with attaching together
4 are set, the fermentation vat 2 is separately connected 21 lower end of 22 lower end of sample arm and liquid adding arm including sample arm 22, liquid adding arm 21, both ends
Pond body 23, the upper end import of liquid adding arm adapting device 4 and liquid adding arm 21 cooperates, and pads 1 buckle closure of lid in 22 upper end opening of sample arm
And it is fixed by impregnating locked instrument 3.
Preferably, 22 upper end opening of sample arm has the annular protrusive board 24 to extend horizontally outward.Sample to be tested
It divides and is placed on annular protrusive board 24, then covered again with pad above.
Preferably, 2 locked instrument of fermentation vat is spiral locking or buckle-type locking or circle hoop formula locking.
Preferably, 21 height of liquid adding arm is greater than 22 height of sample arm.When test, the soak poured into is full of liquid feeding
Arm 21 is greater than the height in sample arm 22 in the height in liquid adding arm 21.Since most soak is in the lower section of sample, institute
It is smaller with the pressure generated by soak, reduce the risk that soak is leaked out from sample edge.
Preferably, when soak liquid volume added reaches 22 height of sample arm in fermentation vat 2, sample exposed face at sample arm 22
The ratio between long-pending and 2 liquid volume added of fermentation vat is not less than 0.1cm-2/mL。
When sample exposed area refers to that sample is in sample arm 22 at sample arm 22 described in this patent, sample does not have
It is contacted with sample arm 22, it is contemplated that and the area of soak contact.
Preferably, the volume of the fermentation vat 2 is greater than 200mL, and works as soak liquid volume added in fermentation vat 2 and reach sample
When product 22 height of arm, liquid volume added is less than 200mL.When i.e. the soak of 200mL is added in fermentation vat 2, it is full of in sample arm 22
Soak, and the height of soak is higher than the height in sample arm 22 in liquid adding arm 21, and then ensures soak and sample
Product come into full contact with.
Preferably, the volume of the fermentation vat 2 is 250mL~350mL.
Preferably, sample exposed area is greater than 0.25dm at the sample arm 222.It is further preferred that the sample arm
Place's sample exposed area is 1.2dm2。
Preferably, the pad lid 1, fermentation vat 2 are made of glass.
Preferably, it is provided with seal groove 11 on the pad lid 1, sealing ring 6 is provided in seal groove 11.It uses
When, sample test faces the placement of sample arm 22, and the pad lid 1 equipped with 6 one side of sealing ring covers on sample.The setting of sealing ring 6, can
To reduce the risk of leakage of analogies during the test；It is contacted moreover, analogies are not direct in test process with sealing ring 6,
Reduce the disturbing factor in test process.
Preferably, the sealing ring 6 is made of silicon rubber or fluoroplastics.Fluorine material can reduce food to greatest extent
The influence that analogies may cause test result, effect are more preferable.
Preferably, sample exposed area is not less than 0.25dm within the seal groove 112.It is close described in this patent
Sample exposed area is the face that thumb pad lid 1 is bonded with 22/ sample of sample arm within seal slot 11, with the one of 11 width of seal groove
It is started at half, calculates the area in seal groove 11.Such as pad the face that lid 1 is bonded with 22/ sample of sample arm, the shape of seal groove 11
Shape is annulus, and interior diameter of a circle is 0.8dm, and outer diameter of a circle is 1dm, then is calculating sample exposed area within seal groove 11
When take diameter for 0.9dm calculating.
Preferably, the pond body 23 is flat.Migration cell can be placed in the horizontal plane by the flat stabilization of pond body 23.
Preferably, the sample arm 22 or liquid adding arm 21 are cased with outside is fixedly connected with block 5.When carrying out heating water bath, due to
The effect of water may be such that migration cell topples over, therefore can be connected by being fixedly connected with block with fixed device, to migration cell carry out into
The fixation of one step.
Preferably, the liquid adding arm adapting device 4 is Chi Gai or condensing reflux pipe.The Chi Gai is glass or fluorine material
Material is made, and condensing reflux pipe is glass cold traps.The liquid adding arm adapting device primarily serves the work of sealing, condensing reflux
With.
The invention also discloses a kind of application methods of above-mentioned migration cell, which is characterized in that comprises the following steps:
(a) sample is placed in sample arm 22, sample test faces sample arm 22, covers pad lid 1, is locked using impregnating
Device 3 will pad lid 1 and fermentation vat 2 locks；
(b) soak is added by liquid adding arm 21；
(c) it is tested.
When necessary, bleeding is carried out after soak is added, contacts sample with soak better.In general, row
Only migration cell need to suitably be tilted toward 22 side of sample arm when gas, exclude remaining air in sample arm 22.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Claims (10)
1. a kind of migration cell, which is characterized in that including pad lid, U-shaped fermentation vat, impregnate locked instrument, the fermentation vat
The pond body of sample arm lower end and liquid adding arm lower end is separately connected including sample arm, liquid adding arm, both ends, pad cover buckle is covered in sample arm
End opening and by impregnate locked instrument fix.
2. a kind of migration cell according to claim 1, which is characterized in that the sample arm upper end opening has horizontal direction
The annular protrusive board of outer extension.
3. a kind of migration cell according to claim 1, which is characterized in that the fermentation vat locked instrument is spiral lock
Gu, buckle-type locking or circle hoop formula locking one of.
4. a kind of migration cell according to claim 1, which is characterized in that it is high that the liquid adding arm height is greater than sample arm
Degree.
5. a kind of migration cell according to claim 1 or 4, which is characterized in that when soak liquid volume added reaches in fermentation vat
When sample arm height, the ratio between sample exposed area and fermentation vat liquid volume added are not less than 0.1cm at sample arm2/mL。
6. a kind of migration cell according to claim 4, which is characterized in that the volume of the fermentation vat is greater than 200mL, and
And when soak liquid volume added reaches sample arm height in fermentation vat, liquid volume added is less than 200mL.
7. a kind of migration cell according to claim 1, which is characterized in that the pad, which covers, is provided with seal groove, close
Sealing ring is provided in seal slot.
8. a kind of migration cell according to claim 1, which is characterized in that the bottom of pond is flat and/or described sample
It is cased with outside product arm or liquid adding arm and is fixedly connected with block.
9. a kind of migration cell according to claim 1, which is characterized in that the upper end import of liquid adding arm is connected with liquid feeding
Arm adapting device.
10. a kind of application method of above-mentioned migration cell, which is characterized in that comprise the following steps:
(a) sample is placed in sample arm, sample test faces sample arm, covers pad lid, will be padded using locked instrument is impregnated
Lid and fermentation vat locking；
(b) soak is added by liquid adding arm；
(c) it is tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811287116.2A CN109297791B (en) | 2018-10-31 | 2018-10-31 | Migration pool and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811287116.2A CN109297791B (en) | 2018-10-31 | 2018-10-31 | Migration pool and use method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN109297791A true CN109297791A (en) | 2019-02-01 |
| CN109297791B CN109297791B (en) | 2024-04-16 |
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| CN201811287116.2A Active CN109297791B (en) | 2018-10-31 | 2018-10-31 | Migration pool and use method thereof |
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| CN104330339A (en) * | 2014-11-08 | 2015-02-04 | 内蒙古农业大学 | Simulator for solute migration under freeze-thaw actions |
| US20160135635A1 (en) * | 2014-11-15 | 2016-05-19 | Michael Boniello | Apparatus for infusing a product with an herb |
| CN204685125U (en) * | 2015-06-11 | 2015-10-07 | 高建国 | A kind of perfluor class that is used for moves determination experiment instrument |
| CN205032431U (en) * | 2015-09-29 | 2016-02-17 | 晶科能源有限公司 | Silicon material soaks belt cleaning device |
| CN105334140A (en) * | 2015-11-30 | 2016-02-17 | 中华人民共和国东莞出入境检验检疫局 | Food contact material single-face soaking device |
| CN206671045U (en) * | 2017-03-28 | 2017-11-24 | 四川出入境检验检疫局检验检疫技术中心 | Food contact sheet material specific transfer experiment sampling infuser device |
| CN206990303U (en) * | 2017-05-12 | 2018-02-09 | 中华人民共和国东莞出入境检验检疫局 | A kind of food contact material two-sided immersion device |
| CN207751764U (en) * | 2017-05-12 | 2018-08-21 | 中华人民共和国东莞出入境检验检疫局 | A kind of film based food contact material two-sided immersion device |
| CN209102489U (en) * | 2018-10-31 | 2019-07-12 | 海宁市产品质量监督检验所（浙江省第一茧质检定所） | A kind of migration cell |
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