CN109294929A - A kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation - Google Patents
A kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation Download PDFInfo
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- CN109294929A CN109294929A CN201811276330.8A CN201811276330A CN109294929A CN 109294929 A CN109294929 A CN 109294929A CN 201811276330 A CN201811276330 A CN 201811276330A CN 109294929 A CN109294929 A CN 109294929A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
- A23F3/10—Fermentation with addition of microorganisms or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
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Abstract
The present invention is about the mushroom cultural method of dark green tea fermentation, and the preparation method of especially a kind of coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation, it is as follows that the method comprising the steps of: coronoid process dissipate capsule bacterium seed culture；The preparation of coronoid process dissipate capsule bacterium solid fermentation；The preparation of coronoid process dissipate capsule bacterium bacterium powder.The solid fermentation culture of the matrix such as this method wheat bran progress strain, and liquid phase low-temperature vacuum dehydration is carried out to culture, the pure culture bacterium powder that moisture content is lower than 8% is obtained, convenient for using in the production of extensive pure-blood ferment dark green tea, and obtained strain survival rate height and fermentation rate are high.
Description
Technical field
Mushroom cultural method of the present invention about dark green tea fermentation, the scattered capsule of especially a kind of coronoid process for dark green tea fermentation
The preparation method of bacterium bacterium powder.
Background technique
Coronoid process dissipate capsule bacterium (Eurotium cristatum) is that China's one kind isolated from traditional fu-brick dark tea is true
Bacterium is commonly called as " golden flower " bacterium, is a valuable feature for this fermented tea.Fu-brick tea production technology is unique, have promote the production of body fluid to quench thirst,
Disappear the physiological functions such as stagnant weight-reducing, especially more prominent with tealeaves effect by the fermentation of appropriate coronoid process dissipate capsule bacterium, this and the strain
Active metabolite and complicated physical and chemical effect between the proper constituent in tealeaves are inseparable.Due to the fermentation being inoculated with naturally
Black brick tea fermentation process in golden flower bacterium generation and purity there is very big uncertainty, and generation toxin may be infected
Other moulds and influence tealeaves flavor bacillus.Therefore the coronoid process dissipate capsule bacterium spore of artificial infection high-purity, high vigor
Powder is to establish golden flower bacterium growth vigor, improves the effective way of tealeaves flavor, quality and effect.
Coronoid process dissipate capsule bacterium category Aspergillus glaucus category, widely distributed, growth substrate includes soil, Fu-brick tea, cordyceps sinensis, Chinese medicine
Piece and sawdust.Coronoid process dissipate capsule bacterium is made of ascocarp and mycelia, and ascocarp, that is, yellow spherical or subsphaeroidal cleistothecium, diameter are about
150~200 μm, ascocarp rupture release biconvex mirror shape ascospore has wide about 0.8~1.0 μm of coronal process, the bacterium
Phorozoon category aspergillus, referred to as coronule aspergillus is P.drechsleri bacterium.Sexual spore is produced under Hyposmolality induction, hypertonic
Pressure induction is lower thoroughly generates asexual spore, generates asexual spore under hyperosmosis induction.
There are strains to live as the fermentation of two generation dark green tea inoculums progress dark green tea for the dark green tea of the common spontaneous fermentation for using maturation
Power is low, the low problem of fermentation success rate.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of preparations of coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation
Method.
The technical solution that the present invention takes to achieve the above object are as follows:
A kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation, it is as follows that the method comprising the steps of:
(1) coronoid process dissipate capsule bacterium seed culture: the spore inoculating on purebred coronoid process dissipate capsule bacterium inclined-plane is leached in wheat bran first
On wheat juice agar medium, with environment culture 4~7 days of 30 DEG C, then mature cleistothecium is washed down with sterile saline,
The broken cleistothecium of bead oscillation is added in inoculation bottle and obtains spore suspension；
(2) prepared by coronoid process dissipate capsule bacterium solid fermentation: the water of 50% weight will be added in fresh wheat bran, is dispensed into tool porous plug
In culture bottle, 125 DEG C degrees Celsius lower steam sterilizing 1 hour, obtain the solid medium for coronoid process dissipate capsule bacterium culture；Then,
According to every gram of culture medium 10 after culture medium naturally cools to room temperature-1~10-3Inoculum concentration access spore suspension, after mixing
It is placed in stationary culture at 30 DEG C, wherein every 48 hours shaking culture bottles to break up agglomeration, loose culture is trained after 8 days
Feeding to look for pool that bottle green is presented, culture terminates to arrive mature solid culture；
(3) it is dry that mature solid culture the preparation of coronoid process dissipate capsule bacterium bacterium powder: is transferred to the Sterile vacuum with external heating
It in room, is heated under vacuum degree appropriate, until the moisture content of material is reduced to 8% or less.Then the vibration in hothouse is utilized
The conidia powder that sieve screening is separated from solid matrix is swung, food is fitted into and is vacuum-packed in aluminium foil bag, that is, obtaining can
With the pure culture activity coronoid process dissipate capsule bacterium bacterium powder of long-term preservation.
The present invention provides a kind of preparation method of coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation, the bases such as this method wheat bran
Matter carries out the solid fermentation culture of strain, and carries out liquid phase low-temperature vacuum dehydration to culture, obtains moisture content and is lower than 8%
Pure culture bacterium powder, convenient for using in the production of extensive pure-blood ferment dark green tea, and obtained strain survival rate is high and ferments
Rate is high.
Specific embodiment
A detailed description is done to a specific embodiment of the invention below.
Embodiment 1: the seed culture of coronoid process dissipate capsule bacterium product:
1, malt extract 9% will be contained, the solid medium of agar 2% is divided in every 250ml eggplant bottle (every after dissolving by heating
Bottle 40mL), 121 DEG C of sterilizing 30min.The eurotium cristatum spore suspension of 1mL is accessed after horizontal cooled and solidified, cultivates 7 at 30 DEG C
It, obtains the coronoid process dissipate capsule bacterium inoculum of cleistothecium riotous growth.
2,50mL sterile saline is added in eggplant bottle, the cleistothecium on culture surface is washed down, is transferred to band gear
In the sterile triangular flask of plate, it is placed in oscillation 20min on oscillator and obtains spore suspension.With haemocytometer to the spore suspension
Liquid is counted, and the spore concentration in sample is 2.5 × 10-7/mL.By microexamination and plate culture, obtained spore
Suspension is uniform, and no living contaminants can be used as the inoculum of bacterium powder solid fermentation.
The solid fermentation culture of coronoid process dissipate capsule bacterium:
The fresh wheat bran of 1kg is taken, the water of 500g is added, is fitted into the culture bottle of 10L after mixing evenly, after being stoppered ventilative tampon
Sterilize 60min at 125 DEG C.5mL spore inoculating object is aseptically accessed after cooling, is cultivated 7 days at 30 DEG C after mixing,
Obtain the solid fermentation culture of the coronoid process dissipate capsule bacterium of dark brown green.
The drying of the solid fermentation object of coronoid process dissipate capsule bacterium and the preparation of bacterium powder:
1, cultured coronoid process dissipate capsule bacterium solid culture is transferred in external heat vacuum desiccator in desinfection chamber, control adds
Hot temperature≤35 DEG C, vacuum chamber pressure≤10kPa stir on one side under conditions of keeping material not occur and freeze agglomeration, on one side
Evaporation drying makes the moisture content of material be reduced to 8% or less from 60%.
2, after moisture content reaches requirement, separating main group using the comb shape sieve bottom sieve on drier becomes the scattered capsule of coronoid process
The yellow fine powder of bacterium spore, is vacuum-packed with vacuum packaging bag.
3, the analysis result of coronoid process dissipate capsule bacterium conidia powder: total spore count 2.0 × 109/ g, spore count 1.45 × 10 living9/ g, spore
Sub- survival rate 72.5%.
Embodiment 2: comparative examples 1 are freeze-dried the bacterium powder preparation of coronoid process dissipate capsule bacterium
1, it is moved into freeze-dryer after freezing cultured coronoid process dissipate capsule bacterium solid culture at -80 DEG C, control heating temperature
≤ 35 DEG C of degree, vacuum chamber pressure≤10Pa is freeze-dried, and the moisture content of material is made to be reduced to 8% or less from 60%.
2, after moisture content reaches requirement, screening is carried out to the material after drying and obtains main group as coronoid process dissipate capsule bacterium
The yellow fine powder of spore, is vacuum-packed with vacuum packaging bag.
3, the analysis result of coronoid process dissipate capsule bacterium conidia powder: total spore count 2.1 × 109/ g, spore count 2.6 × 10 living8/ g, spore
Sub- survival rate 12.5%.
Embodiment 3: 1-room temperature of comparative examples spontaneously dries the bacterium powder preparation of coronoid process dissipate capsule bacterium
1, cultured coronoid process dissipate capsule bacterium solid culture is carried out in 45 DEG C of aeration cabinet with drying is divided, in 30min
It is interior, so that the moisture content of material is reduced to 8% or less from 60%.
2, after moisture content reaches requirement, screening is carried out to the material after drying and obtains main group as coronoid process dissipate capsule bacterium
The yellow fine powder of spore, is vacuum-packed with vacuum packaging bag.
3, the analysis result of coronoid process dissipate capsule bacterium conidia powder: total spore count 1.8 × 109/ g, spore count 1.5 × 10 living8/ g, spore
Sub- survival rate 8.33%.
Claims (1)
1. a kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation, which is characterized in that the method comprising the steps of such as
Under:
(1) coronoid process dissipate capsule bacterium seed culture: the spore inoculating on purebred coronoid process dissipate capsule bacterium inclined-plane is leached in wheat bran first
On wheat juice agar medium, with environment culture 4~7 days of 30 DEG C, then mature cleistothecium is washed down with sterile saline,
The broken cleistothecium of bead oscillation is added in inoculation bottle and obtains spore suspension；
(2) prepared by coronoid process dissipate capsule bacterium solid fermentation: the water of 50% weight will be added in fresh wheat bran, is dispensed into tool porous plug
In culture bottle, 125 DEG C degrees Celsius lower steam sterilizing 1 hour, obtain the solid medium for coronoid process dissipate capsule bacterium culture；Then,
According to every gram of culture medium 10 after culture medium naturally cools to room temperature-1~10-3Inoculum concentration access spore suspension, mix
It is placed on stationary culture at 30 DEG C, wherein every 48 hours shaking culture bottles to break up agglomeration, loose culture, after 8 days
Bottle green is presented in culture color, and culture terminates to arrive mature solid culture；
(3) it is dry that mature solid culture the preparation of coronoid process dissipate capsule bacterium bacterium powder: is transferred to the Sterile vacuum with external heating
It in room, is heated under vacuum degree appropriate, until the moisture content of material is reduced to 8% hereinafter, then utilizing the vibration in hothouse
The conidia powder that sieve screening is separated from solid matrix is swung, food is fitted into and is vacuum-packed in aluminium foil bag, that is, obtaining can
With the pure culture activity coronoid process dissipate capsule bacterium bacterium powder of long-term preservation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811276330.8A CN109294929A (en) | 2018-10-30 | 2018-10-30 | A kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation |
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| CN201811276330.8A CN109294929A (en) | 2018-10-30 | 2018-10-30 | A kind of preparation method of the coronoid process dissipate capsule bacterium bacterium powder for dark green tea fermentation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943438A (en) * | 2019-04-10 | 2019-06-28 | 福建师范大学 | A kind of health red rice method for preparing medicated wine of addition coronoid process dissipate capsule bacterium fermentation |
| CN114431312A (en) * | 2022-01-24 | 2022-05-06 | 浙江欣诺医药有限公司 | Black tea active powder and solid beverage thereof |
Citations (4)
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|---|---|---|---|---|
| CN102120964A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Preparation method of eurotium cristatum spore suspension |
| CN102965328A (en) * | 2012-11-19 | 2013-03-13 | 陕西科技大学 | Preparation method of Fuzhuan tea golden flower fungus spore powder |
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
| CN105255807A (en) * | 2014-07-15 | 2016-01-20 | 中粮营养健康研究院有限公司 | Eurotium cristatum conidium preparation method |
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2018
- 2018-10-30 CN CN201811276330.8A patent/CN109294929A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120964A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Preparation method of eurotium cristatum spore suspension |
| CN102965328A (en) * | 2012-11-19 | 2013-03-13 | 陕西科技大学 | Preparation method of Fuzhuan tea golden flower fungus spore powder |
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
| CN105255807A (en) * | 2014-07-15 | 2016-01-20 | 中粮营养健康研究院有限公司 | Eurotium cristatum conidium preparation method |
Non-Patent Citations (1)
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| 曹聪等: "不同原料及生长因子对冠突散囊菌固体培养特性的影响", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943438A (en) * | 2019-04-10 | 2019-06-28 | 福建师范大学 | A kind of health red rice method for preparing medicated wine of addition coronoid process dissipate capsule bacterium fermentation |
| CN114431312A (en) * | 2022-01-24 | 2022-05-06 | 浙江欣诺医药有限公司 | Black tea active powder and solid beverage thereof |
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Application publication date: 20190201 |