CN109293802A - A kind of agaropectin oligose iron and preparation method thereof - Google Patents
A kind of agaropectin oligose iron and preparation method thereof Download PDFInfo
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- CN109293802A CN109293802A CN201811067294.4A CN201811067294A CN109293802A CN 109293802 A CN109293802 A CN 109293802A CN 201811067294 A CN201811067294 A CN 201811067294A CN 109293802 A CN109293802 A CN 109293802A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
Abstract
It is that chelating, UF membrane, freeze-drying and crushing are assisted by purification, acid degradation, high-pressure pulse electric, agaropectin oligose iron is made using gracilaria verrucosa agar gel as raw material the invention discloses a kind of agaropectin oligose iron and preparation method thereof.Purification is obviously improved neutral sugar content, after acid degradation, promotes the chelating of agaropectin oligose and iron ion under high-pressure pulse electric catalytic action.The technology of the present invention is advanced, reasonable, high production efficiency；The agaropectin oligose iron product iron content of preparation is high, because having good inoxidizability that can promote cell viability, therefore can promote absorption of the body to iron, and bioavilability is high.
Description
Technical field
The present invention relates to food processing fields, more particularly, to the preparation method of agaropectin oligose iron.
Background technique
Iron is one of the essential trace elements of the human body, it is to constitute the essential ingredient of ferroheme, the oxygen in human body
Conveying, Synthesis of DNA and energetic supersession etc. develop important role.Asiderosis can lead to hypoferric anemia
Or functional disturbance.Some researches show that, the whole world with the presence of 30% population asiderosis, 46% 5 ~ 14 years old child and 48% it is pregnant
Woman suffers from hypoferric anemia.Currently, the prevention and treatment of hypoferric anemia is mainly realized by intake iron supplementary.Common iron supplementary has
Ferrous sulfate, frerrous chloride, ferrous gluconate etc., although these iron supplementary iron contents are high, stability is poor, is also easy to produce trip
It from iron, easily generates free radicals in vivo, leads to lipid peroxidation；And there are special metal iron rust taste, mouthfeel bad, easily cause
Gastrointestinal side effect.
Agaropectin oligose has good water solubility, is easily absorbed by the body；Agaropectin oligose contains great amount of hydroxy group and carboxyl, can be with
Metal ion forms stable coordination effect, therefore agaropectin oligose is the ideal material for processing iron chelate, on the one hand can be promoted
Product stability, another aspect agaropectin oligose have good oxidation resistance, can activating cell, to promote body to iron
It absorbs.
Therefore, the present invention develops agaropectin oligose iron chelate and preparation method thereof, and iron is absorbed and utilized to body is improved
Rate is of great significance, and application prospect is good.
Summary of the invention
It is an object of the invention to be directed to the protrusion that the bioavailability of existing supplements-iron product is low, product function is single
Problem provides a kind of agaropectin oligose iron and preparation method thereof, and iron content is higher in product, bioavailability is high.
For achieving the above object, the present invention adopts the following technical scheme:
A kind of preparation method of agaropectin oligose iron, comprising: the purification of gracilaria verrucosa agar gel, acid degradation, high-pressure pulse electric auxiliary chelating,
Agaropectin oligose iron is made in UF membrane, freeze-drying and crushing.
Specific step is as follows:
(1) gracilaria verrucosa agar gel: being configured to the glue of 3 ~ 5wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Fully dissolved, into glue, addition and glue mass ratio are 10% ~ 20% Macrogol 6000 and 1% NaCl clarifying agent, using super
Sonic wave vibrator handles 3min ~ 8min with 20kHz ~ 40kHz, is cooled to room temperature, is centrifuged after staticly settling, takes separating liquid with identical
Clarification, ultrasonic activation processing be centrifuged after staticly settling again, merge sediment, secondary with cold water washing precipitate, filters pressing is de-
Water obtains purification agar-agar；
(2) acid degradation: being configured to the glue of 3 ~ 5wt% for agar-agar is refined with ultrapure water, is heated to 90 DEG C of stirrings to being completely dissolved,
Final concentration of 1.0 mol/L HCl solution, processing 90min is added, dropwise addition 1.0mol/L NaOH solution to pH is 7,7000 r/
Min is centrifuged 15min, takes supernatant, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium makes agaropectin oligose (by dry weight): iron ion: the mass ratio 7.5 ~ 2.5: 1: 7.5 ~ 0.83 of two citric acid monohydrate trisodiums,
PH to 3 ~ 7 is adjusted using HCl or NaOH, after being heated to 50 DEG C ~ 80 DEG C, is handled using the kV/cm high voltage electric field of 20kV/cm ~ 60
30min ~ 60min obtains reaction solution；
(4) UF membrane: using molecular cut off for the ultrafiltration membrance filter reaction solution of 3000Da, collects permeate molecular cut off
For the ultrafiltration membrance filter of 500Da, gained trapped fluid is agaropectin oligose ferrous solution；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
Remarkable advantage of the invention:
(1) using Macrogol 6000 and NaCl, in conjunction with ultrasonic activation processing, impurity in gracilaria verrucosa agar gel and non-is effectively removed
Neutral sugar establishes material base to prepare good agaropectin oligose iron product；(2) agaropectin oligose is catalyzed using high-pressure pulse electric
With the chelating of iron ion, effectively shortens and chelate the time, improve chelation percent；(3) second level UF membrane is used, realizes that high activity agar-agar is few
The separation of sugared iron guarantees product bioavailability；(4) stability of agaropectin oligose iron chelate produced by the present invention is good, agar-agar
Oligosaccharides have good oxidation resistance, can activating cell, to promote absorption of the body to iron.
Detailed description of the invention
Fig. 1 is the infrared spectrogram of agaropectin oligose iron and agaropectin oligose.
Agaropectin oligose is in 1725 cm-1Locate the absorption peak with carboxyl, and disappeared in agaropectin oligose iron spectrum, is shown
Carboxyl in agaropectin oligose may take part in chelatropic reaction as the chelating site with iron in ferric trichloride.In addition, with agar-agar widow
Sugar is compared, and agaropectin oligose iron is in 3385 cm-1The absorption peak at place is more sharp, and in 1605 cm-1With 1386 cm-1The absorption at place
Peak is moved respectively to 1636 cm-1With 1412 cm-1Place, shows that hydroxyl takes part in the chelating of agaropectin oligose and iron.Particularly, fine jade
There are 853,640,594 and 556 cm in glue oligosaccharides iron spectrum-1Four new peaks at place, show carbohydrate fingerprint according to the study
Region (1000 ~ 400 cm-1) some characteristic absorption peaks be specific to β-FeOOH.It is indicated above agaropectin oligose and iron is sent out
Chelatropic reaction is given birth to.
Specific embodiment
Method of the invention is realized especially by following technical step:
A kind of preparation method of agaropectin oligose iron, specific steps are as follows:
(1) gracilaria verrucosa agar gel: being configured to the glue of 3 ~ 5wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Fully dissolved, into glue, addition and glue mass ratio are 10% ~ 20% Macrogol 6000 and 1% NaCl clarifying agent, using super
Sonic wave vibrator handles 3min ~ 8min with 20kHz ~ 40kHz, is cooled to room temperature, is centrifuged after staticly settling, takes separating liquid with identical
Clarification, ultrasonic activation processing be centrifuged after staticly settling again, merge sediment, secondary with cold water washing precipitate, filters pressing is de-
Water obtains purification agar-agar；
(2) acid degradation: being configured to the glue of 3 ~ 5wt% for agar-agar is refined with ultrapure water, is heated to 90 DEG C of stirrings to being completely dissolved,
Final concentration of 1.0 mol/L HCl solution, processing 90min is added, dropwise addition 1.0mol/L NaOH solution to pH is 7,7000 r/
Min is centrifuged 15min, takes supernatant, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium makes agaropectin oligose (by dry weight): iron ion: the mass ratio 7.5 ~ 2.5: 1: 7.5 ~ 0.83 of two citric acid monohydrate trisodiums,
PH to 3 ~ 7 is adjusted using HCl or NaOH, after being heated to 50 DEG C ~ 80 DEG C, is handled using the kV/cm high voltage electric field of 20kV/cm ~ 60
30min ~ 60min obtains reaction solution；
(4) UF membrane: using molecular cut off for the ultrafiltration membrance filter reaction solution of 3000Da, collects permeate molecular cut off
For the ultrafiltration membrance filter of 500Da, gained trapped fluid is agaropectin oligose ferrous solution；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
In order to sufficiently disclose the method that the present invention prepares oligosaccharides iron complexes, it is illustrated below in conjunction with example.
Embodiment 1
(1) gracilaria verrucosa agar gel: being configured to the glue of 5wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Dissolution, into glue, addition and glue mass ratio are 20% Macrogol 6000 and 1% NaCl clarifying agent, are shaken using ultrasonic wave
Dynamic device handles 3min with 40kHz, is cooled to room temperature, is centrifuged after staticly settling, takes separating liquid with identical clarification, ultrasonic activation
Processing is centrifuged after staticly settling again, merges sediment, secondary with cold water washing precipitate, filter-press dehydration obtains purification agar-agar；
(2) purification agar-agar: being configured to the glue of 5wt% with ultrapure water by acid degradation, is heated to 90 DEG C of stirrings to being completely dissolved, is added
Enter final concentration of 1.0 mol/L HCl solution, processing 90min, dropwise addition 1.0mol/L NaOH solution to pH is 7,7000 r/min
It is centrifuged 15min, supernatant is taken, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium makes agaropectin oligose (by dry weight): iron ion: the mass ratio 7.5: 1: 7.5 of two citric acid monohydrate trisodiums, using HCl tune
PH to 3 is saved, after being heated to 50 DEG C, 60min is handled using 20kV/cm high voltage electric field, obtains reaction solution；
(4) UF membrane: using molecular cut off for the ultrafiltration membrance filter reaction solution of 3000Da, collects permeate molecular cut off
For the ultrafiltration membrance filter of 500Da, gained trapped fluid is agaropectin oligose ferrous solution；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
The agaropectin oligose iron prepared using this example, weight average molecular weight 1252Da, wherein iron content is 12.83%.
Embodiment 2
(1) gracilaria verrucosa agar gel: being configured to the glue of 4wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Dissolution, into glue, addition and glue mass ratio are 15% Macrogol 6000 and 1% NaCl clarifying agent, are shaken using ultrasonic wave
Dynamic device handles 5min with 30kHz, is cooled to room temperature, is centrifuged after staticly settling, takes separating liquid with identical clarification, ultrasonic activation
Processing is centrifuged after staticly settling again, merges sediment, secondary with cold water washing precipitate, filter-press dehydration obtains purification agar-agar；
(2) purification agar-agar: being configured to the glue of 4wt% with ultrapure water by acid degradation, is heated to 90 DEG C of stirrings to being completely dissolved, is added
Enter final concentration of 1.0 mol/L HCl solution, processing 90min, dropwise addition 1.0mol/L NaOH solution to pH is 7,7000 r/min
It is centrifuged 15min, supernatant is taken, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium makes agaropectin oligose (by dry weight): iron ion: the mass ratio 5: 1: 2.5 of two citric acid monohydrate trisodiums is adjusted using NaOH
PH to 5 after being heated to 65 DEG C, handles 45min using 40 kV/cm high voltage electric fields, obtains reaction solution；
(4) UF membrane: using molecular cut off for the ultrafiltration membrance filter reaction solution of 3000Da, collects permeate molecular cut off
For the ultrafiltration membrance filter of 500Da, gained trapped fluid is agaropectin oligose ferrous solution；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
The agaropectin oligose iron prepared using this example, weight average molecular weight 1355Da, wherein iron content is 14.03%.
Embodiment 3
(1) gracilaria verrucosa agar gel: being configured to the glue of 3wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Dissolution, into glue, addition and glue mass ratio are 10% Macrogol 6000 and 1% NaCl clarifying agent, are shaken using ultrasonic wave
Dynamic device handles 8min with 20kHz, is cooled to room temperature, is centrifuged after staticly settling, takes separating liquid with identical clarification, ultrasonic activation
Processing is centrifuged after staticly settling again, merges sediment, secondary with cold water washing precipitate, filter-press dehydration obtains purification agar-agar；
(2) purification agar-agar: being configured to the glue of 3wt% with ultrapure water by acid degradation, is heated to 90 DEG C of stirrings to being completely dissolved, is added
Enter final concentration of 1.0 mol/L HCl solution, processing 90min, dropwise addition 1.0mol/L NaOH solution to pH is 7,7000 r/min
It is centrifuged 15min, supernatant is taken, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium makes agaropectin oligose (by dry weight): iron ion: the mass ratio 2.5: 1: 0.83 of two citric acid monohydrate trisodiums, using NaOH
PH to 7 is adjusted, after being heated to 80 DEG C, 30min is handled using 60 kV/cm high voltage electric fields, obtains reaction solution；
(4) UF membrane: using molecular cut off for the ultrafiltration membrance filter reaction solution of 3000Da, collects permeate molecular cut off
For the ultrafiltration membrance filter of 500Da, gained trapped fluid is agaropectin oligose ferrous solution；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
The agaropectin oligose iron prepared using this example, weight average molecular weight 1196Da, wherein iron content is 11.26%.
Test
60 SD rats are randomly divided into Normal group (12) and iron deficiency model group (48).Normal group fed control
Feed, iron deficiency model group feed low iron feed.All rat ad libs, after feeding 28 days, when hemoglobin (Hb) is less than 70
When g/L, i.e., hypoferric anemia models successfully.Then model group rats are divided into iron deficiency model group, ferrous gluconate group, sulfuric acid
Ferrous iron group and agaropectin oligose iron group, every group each 12.Ferrous gluconate group and ferrous sulfate group rat distinguish stomach-filling glucose
Sour ferrous and ferrous sulfate solution, agaropectin oligose iron group rat feed the agaropectin oligose ferrous solution of a half-value dose, iron deficiency model group
With the isometric physiological saline of rats in normal control group stomach-filling, daily stomach-filling is primary, and continuous gavage 28 days.Always except Normal group
Outside fed control feed, remaining group feeds low iron feed.After the test, fasting 12 hours, cardiac puncture collect blood sample,
Blood routine test is carried out, the results are shown in Table 1.
Benefit iron Contrast on effect of the different supplements-irons of table 1 to iron deficiency rat
Note: same row shoulder mark lowercase, letter is different to indicate significant difference (P < 0.05) between the different sets.
Hb, RBC and HCT respectively indicate blood routine main indicator in table 1 --- hemoglobin, red blood cell, hematocrit value.
Compared with ferrous gluconate, ferrous sulfate, the agaropectin oligose iron for taking a half-value dose can reach comparable benefit iron effect, make
The blood routine of iron deficiency rat restores to normal level.
The foregoing is merely preferred embodiments of the invention, all equivalent changes done according to scope of the present invention patent with repair
Decorations, are all covered by the present invention.
Claims (7)
1. a kind of preparation method of agaropectin oligose iron, which comprises the following steps:
(1) gracilaria verrucosa agar gel: being configured to the glue of 3 ~ 5wt% with ultrapure water by the purification of gracilaria verrucosa agar gel, is heated to 90 DEG C and is stirred to complete
Fully dissolved adds clarifying agent into glue, is handled using ultrasonic activation, is cooled to room temperature, and is centrifuged after staticly settling, and takes separation
Liquid is centrifuged after being staticly settled again with identical clarification, ultrasonic activation processing, merges sediment, with cold water washing precipitate two
Secondary, filter-press dehydration obtains purification agar-agar；
(2) acid degradation: being configured to the glue of 3 ~ 5wt% for agar-agar is refined with ultrapure water, is heated to 90 DEG C of stirrings to being completely dissolved,
Final concentration of 1.0 mol/L HCl solution, processing 90min is added, 1.0 mol/L NaOH solutions of dropwise addition to pH are 7,7000 r/
Min is centrifuged 15min, takes supernatant, obtains agaropectin oligose hydrolyzate；
(3) FeCl high-pressure pulse electric auxiliary chelating: is added into agaropectin oligose hydrolyzate3 .6H2O and two citric acid monohydrates three
Sodium is adjusted pH to 3 ~ 7 using HCl or NaOH and is handled using high voltage electric field after being heated to 50 DEG C ~ 80 DEG C, obtain reaction solution；
(4) UF membrane: using ultrafiltration membrane secondary separation, is agaropectin oligose ferrous solution through trapped fluid obtained by secondary separation；
(5) it is freeze-dried and crushes: agaropectin oligose ferrous solution vacuum concentration being placed in vacuum freeze drier, hothouse is exhausted
It is 20Pa to pressure, pre-freeze junction temperature is -35 DEG C, and resolution temperature is 35 DEG C, dry to moisture content 5wt% hereinafter, smashing it through 80
Mesh obtains agaropectin oligose iron.
2. the preparation method of agaropectin oligose iron according to claim 1, which is characterized in that clarifying agent described in step (1)
It is to be formed with glue mass ratio for 10% ~ 20% Macrogol 6000 and 1% NaCl.
3. the preparation method of agaropectin oligose iron according to claim 1, which is characterized in that ultrasonic wave described in step (1)
Vibration processing is to handle 3min ~ 8min with 20kHz ~ 40kHz ultrasonic frequency.
4. the preparation method of agaropectin oligose iron according to claim 1, which is characterized in that be added in step (3)
FeCl3 .6H2O and two citric acid monohydrate trisodiums ratio are the i.e. agaropectin oligoses based on the agaropectin oligose dry weight of agaropectin oligose hydrolyzate
Dry weight: iron ion: the mass ratio of two citric acid monohydrate trisodiums is 7.5 ~ 2.5: 1: 7.5 ~ 0.83.
5. the preparation method of agaropectin oligose iron according to claim 1, which is characterized in that high-voltage electricity described in step (3)
Field processing is to handle 30min ~ 60min with 20kV/cm ~ 60kV/cm high voltage electric field.
6. the preparation method of agaropectin oligose iron according to claim 1, which is characterized in that ultrafiltration described in step (4)
Film secondary separation is to be separated using molecular cut off for the ultrafiltration membrane of 3000Da as first time, collects after filtering reacting liquid and penetrate
Liquid, then second of separation is carried out to permeate for the ultrafiltration membrance filter of 500Da with molecular cut off.
7. agaropectin oligose iron is made in preparation method as claimed in any one of claims 1 to 6.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115336757A (en) * | 2022-07-26 | 2022-11-15 | 华南理工大学 | Method for preparing water-soluble soybean polysaccharide calcium chelate with assistance of high-voltage pulse electric field |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6132750A (en) * | 1998-04-14 | 2000-10-17 | Coletica | Particles of cross-linked proteins and polysaccharides with hydroxamic groups for chelating metals and their uses notably in cosmetics |
| CN1394879A (en) * | 2001-07-05 | 2003-02-05 | 青岛海洋大学 | Agaropectin oligosaccharide and agaropectin oligose preparation method |
| EP0988797B1 (en) * | 1996-10-08 | 2004-02-25 | Meiji Seika Kabushiki Kaisha | Use of compositions for postgastrectomic mineral supply |
| JP2009067688A (en) * | 2007-09-11 | 2009-04-02 | Oji Paper Co Ltd | Iron absorption promoter |
| CN101967205A (en) * | 2010-10-12 | 2011-02-09 | 滨州学院 | Beta-dextriferron composite and preparation method thereof |
-
2018
- 2018-09-13 CN CN201811067294.4A patent/CN109293802B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0988797B1 (en) * | 1996-10-08 | 2004-02-25 | Meiji Seika Kabushiki Kaisha | Use of compositions for postgastrectomic mineral supply |
| US6132750A (en) * | 1998-04-14 | 2000-10-17 | Coletica | Particles of cross-linked proteins and polysaccharides with hydroxamic groups for chelating metals and their uses notably in cosmetics |
| CN1394879A (en) * | 2001-07-05 | 2003-02-05 | 青岛海洋大学 | Agaropectin oligosaccharide and agaropectin oligose preparation method |
| JP2009067688A (en) * | 2007-09-11 | 2009-04-02 | Oji Paper Co Ltd | Iron absorption promoter |
| CN101967205A (en) * | 2010-10-12 | 2011-02-09 | 滨州学院 | Beta-dextriferron composite and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| JOSE MOISES LAPARRA 等: "Structural differences of prebiotic oligosaccharides influence their capability to enhance iron absorption in deficient rats", 《FOOD & FUNCTION》 * |
| KAI MAO等: "Preparation, Characterization, and Antioxidant Activity of an Isomaltooligosaccharide–Iron Complex (IIC)", 《JOURNAL OF CARBOHYDRATE CHEMISTRY》 * |
| 何洪 等: "寡糖金属螯合物的研究进展", 《食品与机械》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115336757A (en) * | 2022-07-26 | 2022-11-15 | 华南理工大学 | Method for preparing water-soluble soybean polysaccharide calcium chelate with assistance of high-voltage pulse electric field |
| CN115336757B (en) * | 2022-07-26 | 2023-06-16 | 华南理工大学 | Method for preparing water-soluble soybean polysaccharide calcium chelate with assistance of high-voltage pulse electric field |
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