CN109291298B - Trace liposome high pressure extrusion device - Google Patents

Trace liposome high pressure extrusion device Download PDF

Info

Publication number
CN109291298B
CN109291298B CN201811155544.XA CN201811155544A CN109291298B CN 109291298 B CN109291298 B CN 109291298B CN 201811155544 A CN201811155544 A CN 201811155544A CN 109291298 B CN109291298 B CN 109291298B
Authority
CN
China
Prior art keywords
extrusion
cylinder
sealing
head
pressure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811155544.XA
Other languages
Chinese (zh)
Other versions
CN109291298A (en
Inventor
叶玉林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Aitesen Pharmaceutical Equipment Co ltd
Original Assignee
Suzhou Aitesen Pharmaceutical Equipment Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Aitesen Pharmaceutical Equipment Co ltd filed Critical Suzhou Aitesen Pharmaceutical Equipment Co ltd
Priority to CN201811155544.XA priority Critical patent/CN109291298B/en
Publication of CN109291298A publication Critical patent/CN109291298A/en
Application granted granted Critical
Publication of CN109291298B publication Critical patent/CN109291298B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B29WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29BPREPARATION OR PRETREATMENT OF THE MATERIAL TO BE SHAPED; MAKING GRANULES OR PREFORMS; RECOVERY OF PLASTICS OR OTHER CONSTITUENTS OF WASTE MATERIAL CONTAINING PLASTICS
    • B29B9/00Making granules
    • B29B9/02Making granules by dividing preformed material
    • B29B9/06Making granules by dividing preformed material in the form of filamentary material, e.g. combined with extrusion

Abstract

The invention relates to a micro-liposome high-pressure extrusion device, which comprises a cylinder assembly and a micro-extrusion container, wherein the cylinder assembly comprises a cylinder bracket, a cylinder, a piston rod and a pressure head assembly, the cylinder is erected on the cylinder bracket, the micro-extrusion container comprises a cylinder body, a seal head and a filtering assembly, the piston rod, the pressure head assembly, the cylinder body and the seal head form a piston extrusion mechanism, a sample inlet is arranged on the side surface of the cylinder body, a sample outlet is arranged at the bottom of the cylinder body, a filter tank is arranged on the top surface of the seal head, the bottom of the seal head is provided with an extrusion outlet, the filtering assembly is filled in the filter tank, and the pressure head assembly comprises a sealing pressure head and an extrusion pressure head, the size of which is matched with the sample outlet, and a heat insulation jacket which is respectively arranged on the outer walls of the cylinder body and the seal head in a circumferential direction; the invention provides a micro-liposome high-pressure extrusion device which can simultaneously meet the liposome sample extrusion requirement of more than 200bar for laboratory use and has less required sample quantity and a perfect heat preservation device.

Description

Trace liposome high pressure extrusion device
Technical Field
The invention relates to the field of pharmacy, in particular to a micro-liposome high-pressure extrusion device.
Background
With the development of the domestic pharmaceutical industry, the varieties of high-end preparations such as targeting and slow release are more and more, and the application of liposome as a micro vesicle similar to a cell membrane structure is more and more extensive, and the liposome has more and more application to liposome technology in the pharmaceutical industry in recent years because of the very good targeting and slow release effects on the prepared preparation. Since liposome vesicles are required to achieve the desired effect, the liposome particles prepared must be subjected to particle size control, and the particle size and distribution of the liposome particles must be controlled within a good range, for example, the particle size is controlled below 100nm, and the PDI distribution is controlled below 0.1.
In the prior art, the whole particle control of the liposome is generally realized by adopting a high-pressure extrusion mode, liposome particles are extruded through a PC polycarbonate filter membrane with a specific pore diameter by a power source, and the particle size of the liposome particles is reduced and the distribution is uniform under the action of the shearing force of the filter membrane. Because laboratory and production technology in the existing market have great differences, laboratory equipment can not achieve the pressure required by better grain arrangement due to the fact that the highest use pressure is too low, so that samples can not be well processed, and in addition, because of the difference of extrusion modes, the laboratory technology has great problems in the process of amplifying to production, such as: 1. when the existing laboratory extrusion device adopts air pressure as a power source for extrusion, the highest pressure generated is only about 70bar, and the extrusion effect can not be achieved for liposome samples which are difficult to integrate; 2. the existing extrusion device capable of realizing high-pressure extrusion (not lower than 200 bar) requires a large amount of sample (not lower than 50 ml), and for the liposome sample with high cost, the cost is too high and the residue is too large when the liposome sample is used in a laboratory; 3. the existing laboratory extrusion device generally only keeps the temperature of the module at the upper end of the extrusion film, and does not keep the temperature of the lower part, so that the temperature of the upper part and the lower part are not uniform easily, and the extrusion effect is greatly affected.
In view of the above-mentioned drawbacks, the present inventors have actively studied and innovated to create a micro-liposome high-pressure extrusion device with a complete heat-insulating device, which can simultaneously meet the working pressure of more than 200bar for laboratory use, and the extrusion requirement of a liposome sample with a small required sample amount (minimum of only 2 ml), and has more industrial utilization value.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a micro-liposome high-pressure extrusion device which can extrude the liposome at a micro-amount under high pressure and can simultaneously keep the temperature.
The invention discloses a micro liposome high-pressure extrusion device, which comprises a cylinder assembly and a micro extrusion container, wherein the cylinder assembly comprises a cylinder bracket, a cylinder, a piston rod and a pressure head assembly, the cylinder is erected on the cylinder bracket, the micro extrusion container comprises a cylinder body, a sealing head and a filtering assembly, the piston rod, the pressure head assembly, the cylinder body and the sealing head form a piston extrusion mechanism, a sample inlet is arranged on the side surface of the cylinder body, a sample outlet is arranged at the bottom of the cylinder body, a filtering groove is arranged on the top surface of the sealing head, the bottom of the sealing head is provided with an extrusion outlet, the filtering assembly is filled in the filtering groove, and the pressure head assembly comprises a sealing pressure head and an extrusion pressure head with the size matched with the sample outlet, and the sealing head is respectively arranged on the outer walls of the cylinder body and the sealing head in a circumferential direction.
Further, the cylinder body and the seal head are fixed through a compression flange.
Further, a plurality of sealing grooves and sealing rings which are matched with each other are formed in the contact surface between the cylinder body and the sealing head.
Further, the extrusion device also comprises an extrusion pipeline connected with the extrusion port.
Further, a sealing groove and a sealing ring matched with the sealing groove are arranged on the outer wall of the sealing pressure head.
Furthermore, the sealing pressure head is in contact sealing with the inner wall of the cylinder body through the sealing ring.
Further, the filter assembly includes a filter membrane and a support plate sequentially arranged in the extrusion direction.
Further, the diameter of the sample outlet is equal to the diameter of the extrusion pressure head.
Further, one end of the piston rod is connected with the cylinder, and the sealing pressure head is fixed at the other end of the piston rod.
By means of the scheme, the invention has at least the following advantages:
1. high-pressure extrusion: the pressure of gas less than 10bar is used as a power source, the pressure boosting conversion is carried out in the equipment, the pressure boosting conversion can be carried out in the equipment, the extrusion working pressure of the equipment is not less than 200bar, in addition, the working cavity is made of 316L stainless steel, the thickness is not less than 25mm, and the pressure-resistant requirement of the 200bar working pressure can be fully met;
2. the sample amount is small: the amount of the sample extruded once is in the range of 2ml to 25ml, the amount of the consumed sample is very small, and the sample can be completely discharged without causing residues.
3. The heat preservation device comprises: when the extrusion device is designed, jacket heat preservation devices are designed on the modules before and after film passing, heat preservation mediums are introduced into the jackets, so that the temperature of the extrusion device can be controlled, and the extrusion process is ensured to be carried out within a required temperature range.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a cross-sectional view of a high pressure extrusion device for micro-liposomes of the present invention;
fig. 2 is a cross-sectional view of the liposome of fig. 1 as it is fully extruded.
1 cylinder support 2 cylinder
3 piston rod 4 cylinder
5 seal head 6 sample inlet
7 sample outlet 8 filter tank
9 extrusion port 10 sealing ram
11 extrusion head 12 thermal insulation jacket
13 compression flange 14 seal groove
15 seal 16 extrusion pipe
17 filter membrane 18 backup pad
Detailed Description
The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
The utility model provides a micro-liposome high pressure extrusion device that figure 1 and figure 2 show, including cylinder 2 subassembly and micro extrusion container, cylinder 2 subassembly includes cylinder support 1, cylinder 2, piston rod 3 and pressure head subassembly, cylinder 2 erects on cylinder support 1, micro extrusion container includes barrel 4, head 5 and filter component, piston rod 3, pressure head subassembly, barrel 4 and head 5 constitute piston extrusion mechanism, barrel 4 side is equipped with sample inlet 6, barrel 4 bottom is equipped with out appearance mouth 7, filter tank 8 has been seted up on the head 5 top surface, head 5 bottom is equipped with extrusion mouth 9, filter tank 8 intussuseption is filled with filter component, the pressure head subassembly includes seal pressure head 10 that follows extrusion direction order and the extrusion pressure head 11 of size and the looks adaptation of appearance mouth 7, still include the thermal-insulation jacket 12 that sets up at barrel 4 and head 5 outer wall respectively in the ring direction.
The principle of the micro liposome high-pressure extrusion device provided by the invention is as follows:
the cylinder 2 arranged on the cylinder support 1 pushes the piston rod 3 to drive the pressure head assembly to enter the inner cavity of the cylinder 4 in the micro extrusion container, the sealing pressure head 10 in the pressure head assembly prevents air pressure leakage in the inner cavity of the cylinder 4, the extrusion pressure head 11 is used for extruding liposome, the liposome enters the inner cavity of the cylinder 4 through the sample inlet 6 arranged on the side surface of the cylinder 4, the sealing pressure head 10 driven by the piston rod 3 is concentrated at the sample outlet 7 at the bottom of the cylinder 4 under the action of the sealing pressure head 10 assembly, when the extrusion pressure head 11 reaches the bottom of the cylinder 4, the top surface of the sealing head 5 in sealing connection with the cylinder 4 is provided with the filter tank 8 corresponding to the sample outlet 7, thus, the extrusion pressure head 11 can slowly extrude the sample outlet 7 of liposome concentrated at the bottom of the cylinder 4 and obtain liposome particles distributed in certain particle size and PDI through the filter assembly filled in the filter tank 8, the liposome particles are discharged from the sample through the extrusion outlet 9 arranged at the bottom of the sealing head 5 in a ring direction respectively at the jacket 12 arranged on the outer wall of the cylinder 4 and the sealing head 5 in the extrusion process, the temperature of the extrusion device can be controlled, the extrusion process is ensured, and the extrusion process is ensured within a required temperature range, and the extrusion effect of the liposome is ensured.
In order to realize high-pressure extrusion of trace liposome, liposome particles with certain particle size and PDI distribution are formed, and the cylinder 4 and the seal head 5 are fixed through a compression flange 13.
In order to prevent pressure leakage, a plurality of sealing grooves 14 and an adaptive sealing ring 15 are arranged on the contact surface between the cylinder body 4 and the sealing head 5.
In order to control the temperature of the extrusion device, ensuring that the extrusion process is carried out within the desired temperature range, an extrusion pipe 16 is also included in connection with the extrusion port 9.
In order to ensure the air tightness in the inner cavity of the cylinder body 4 and ensure the high-pressure extrusion of liposome, a sealing groove 14 and a sealing ring 15 matched with the sealing groove 14 are arranged on the outer wall of the sealing pressure head 10, and the sealing pressure head 10 is in contact sealing with the inner wall of the cylinder body 4 through the sealing ring 15.
In order to achieve extrusion of liposome particles of a certain particle size and PDI distribution for the liposomes, the filter assembly comprises a filter membrane 17 and a support plate 18 arranged sequentially in the extrusion direction.
In order to realize that the liposome to be extruded in the cylinder 4 has no residue, paper body particles can be formed by complete extrusion, and the diameter of the sample outlet 7 is equal to the diameter of the extrusion pressure head 11.
In order to realize automatic precise control of liposome extrusion, one end of a piston rod 3 is connected with a cylinder 2, and the other end of the piston rod 3 is fixed with a sealing pressure head 10.
The above is only a preferred embodiment of the present invention, and it should be noted that it should be understood by those skilled in the art that several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention.

Claims (4)

1. A micro-liposome high-pressure extrusion device, which is characterized in that: comprising a cylinder assembly and micro extrusion
The container, the cylinder subassembly includes cylinder support, cylinder, piston rod and pressure head subassembly, the cylinder erects
On the cylinder support, the micro extrusion container comprises a cylinder body, a sealing head and a filtering component, and the piston
The rod, the pressure head assembly, the cylinder body and the seal head form a piston extrusion mechanism, a sample inlet is arranged on the side surface of the cylinder body, and the piston extrusion mechanism comprises a cylinder body
The bottom of the cylinder body is provided with a sample outlet, the top surface of the sealing head is provided with a filter tank, and the bottom of the sealing head is provided with an extruding device
An outlet, the filter tank is filled with the filter assembly, and the pressure head assembly comprises a sequence along the extrusion direction
The arranged sealing pressure head and the extrusion pressure head with the size matched with the sample outlet also comprise annular devices respectively
A heat-insulating jacket arranged on the outer wall of the cylinder body and the sealing head, a contact surface between the cylinder body and the sealing head is provided with
The sealing head is provided with a plurality of sealing grooves and sealing rings which are matched with the sealing grooves, and the outer wall of the sealing head is provided with the sealing grooves and the sealing rings
The sealing head is contacted and sealed with the inner wall of the cylinder body through the sealing ring which is matched with the sealing groove,
the diameter of the sample outlet is equal to that of the extrusion pressure head, and one end of the piston rod is air-tight
The cylinder is connected, and the sealing pressure head is fixed with the other end of the piston rod.
2. The micro-liposome high-pressure extrusion apparatus according to claim 1, wherein: the cartridge
The body and the seal head are fixed through a compression flange.
3. The micro-liposome high-pressure extrusion apparatus according to claim 1, wherein: and also comprises
And an extrusion pipe connected with the extrusion port.
4. The micro-liposome high-pressure extrusion apparatus according to claim 1, wherein: the said passing
The filter assembly includes a filter membrane and a support plate sequentially arranged in an extrusion direction.
CN201811155544.XA 2018-09-30 2018-09-30 Trace liposome high pressure extrusion device Active CN109291298B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811155544.XA CN109291298B (en) 2018-09-30 2018-09-30 Trace liposome high pressure extrusion device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811155544.XA CN109291298B (en) 2018-09-30 2018-09-30 Trace liposome high pressure extrusion device

Publications (2)

Publication Number Publication Date
CN109291298A CN109291298A (en) 2019-02-01
CN109291298B true CN109291298B (en) 2024-03-29

Family

ID=65161252

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811155544.XA Active CN109291298B (en) 2018-09-30 2018-09-30 Trace liposome high pressure extrusion device

Country Status (1)

Country Link
CN (1) CN109291298B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109925956A (en) * 2019-04-29 2019-06-25 苏州艾特森制药设备有限公司 A kind of laboratory liposome preparation system

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554382A (en) * 1993-05-28 1996-09-10 Aphios Corporation Methods and apparatus for making liposomes
CN202822916U (en) * 2012-09-29 2013-03-27 吉林大学 Extrusion device of tangential flow liposome
CN103192516A (en) * 2013-04-02 2013-07-10 上海正乾生物技术有限公司 High-pressure extruding and filtering system for preparing liposome
CN104225984A (en) * 2014-08-07 2014-12-24 常州海源恒业水处理设备有限公司 Filter press
CN105188674A (en) * 2013-03-14 2015-12-23 生物休眠有限公司 Liposome formulation and manufacture
CN105498543A (en) * 2015-12-15 2016-04-20 中国医学科学院生物医学工程研究所 High pressure extrusion and filtration device for liposome
CN208841627U (en) * 2018-09-30 2019-05-10 苏州艾特森制药设备有限公司 A kind of micro liposome high pressure extrusion device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554382A (en) * 1993-05-28 1996-09-10 Aphios Corporation Methods and apparatus for making liposomes
CN202822916U (en) * 2012-09-29 2013-03-27 吉林大学 Extrusion device of tangential flow liposome
CN105188674A (en) * 2013-03-14 2015-12-23 生物休眠有限公司 Liposome formulation and manufacture
CN103192516A (en) * 2013-04-02 2013-07-10 上海正乾生物技术有限公司 High-pressure extruding and filtering system for preparing liposome
CN104225984A (en) * 2014-08-07 2014-12-24 常州海源恒业水处理设备有限公司 Filter press
CN105498543A (en) * 2015-12-15 2016-04-20 中国医学科学院生物医学工程研究所 High pressure extrusion and filtration device for liposome
CN208841627U (en) * 2018-09-30 2019-05-10 苏州艾特森制药设备有限公司 A kind of micro liposome high pressure extrusion device

Also Published As

Publication number Publication date
CN109291298A (en) 2019-02-01

Similar Documents

Publication Publication Date Title
CN109291298B (en) Trace liposome high pressure extrusion device
CN101108310A (en) Hollow fiber ceramic membrane element and component thereof
CN208841627U (en) A kind of micro liposome high pressure extrusion device
CN201179445Y (en) Tetra-fluorine liner reaction tank with small area expansion stress
CN212091719U (en) Flower disc for ceramic membrane assembly and novel sealing ceramic membrane assembly comprising flower disc
CN215720859U (en) Bleeder valve for pharmaceutical equipment
CN2608933Y (en) Enamel glass heat exchanger
CN210814034U (en) Marigold supercritical CO2Extraction device
CN211562932U (en) Medicine reation kettle with sampling detects function
CN213528613U (en) Oil bath heating reaction kettle for producing ethyl bis (2, 4-dinitrobenzene) acetate
CN110657698B (en) Heat energy utilization device for protein drug extraction
CN102641694B (en) Esterifier
CN217341262U (en) Sealing device for feeding pipe of reaction kettle
CN202561301U (en) Pressurized seal connection piece
CN213611250U (en) High-pressure catalytic reactor
CN203291321U (en) Device automatically exhausting gas and stopping liquid
CN220779323U (en) Filter and filtration system
CN216592227U (en) Prickly ash oil heater with variable volume
CN218250181U (en) Reation kettle is used in chemical material production
CN219595921U (en) Novel hydraulic dynamic axial compression liquid chromatography column
CN217646385U (en) Energy-saving environment-friendly chemical reaction kettle
CN215542319U (en) Valve body unit of glue dispensing device
CN201231096Y (en) Catalyst separation device in reactor
CN213576068U (en) Gas distribution sealing connecting pipe convenient to disassemble and assemble
CN117339485B (en) Carboxylation continuous reaction device

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant