CN109287659A - Lanthanum is as the agent of crop cut cadmium and cadmium transporter gene expression inhibiting agent and the application of increasing agent - Google Patents
Lanthanum is as the agent of crop cut cadmium and cadmium transporter gene expression inhibiting agent and the application of increasing agent Download PDFInfo
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Abstract
The present invention relates to a kind of lanthanums as the agent of crop cut cadmium and cadmium transporter gene expression inhibiting agent and the application of increasing agent.In wheat growth to the jointing stage, apply lanthanum into soil；Again at wheat growth to heading stage, foliage-spray lanthanum is carried out.By imposing lanthanum twice, absorption and transhipment of the wheat to cadmium can be reduced, resistance control wheat aerial part cadmium is transported to seed；Wheat yield can be increased simultaneously.In addition, lanthanum is alternatively arranged as a kind of inhibitor that downward crop expresses Cd uptake transporter gene related with transhipment, applied in wheat during jointing stage from root, is able to suppress the expression that wheat absorbs transporter gene related to transhipment cadmium.
Description
Technical field
The invention belongs to agricultural environment technical fields more particularly to lanthanum as the agent of crop cut cadmium and cadmium transporter gene table
Up to the application of inhibitor and increasing agent.
Background technique
With the quick raising of social production living standard, Industrial " three Waste ", municipal refuse total emission volumn are risen year by year, agriculture
Medicine, chemical fertilizer are abused for a long time and a large amount of uses of municipal sludge, so that the heavy metal pollution in the agricultural soil of some areas is increasingly
Increase severely, seriously threatens the sustainable development of ecological environment security, grain security, national health and economic society.Cadmium (Cd) is
One of most common, strongest heavy metal of toxicity.Its mobility is strong, is easily absorbed by crops, and can largely be enriched in organism, to people
Class health threat is huge, clinically often shows as the diseases such as osteoporosis, canceration, hepatic and renal function disorder and hypertension.
Codex Alimentary Commission sets secure threshold with regard to staple food crop edible part Cd content, such as in wheat
Seed is 0.2mg/kg, rice grain 0.4mg/kg.However, most of staple food crop for being grown in Cd contaminated soil can
The content for eating part Cd has but been more than safety standard.For this status, a set of quickly and effectively integrated control technique is researched and developed, is had
Effect reduces cadmium content in staple food crop such as wheat seed, to ensuring that China or even world food safety and ecological environment pacifies
Entirely, alleviate current census to have practical significance with cultivated land resource contradiction.
Crop yield is improved, crop quality is improved and enhances crop to bad research shows that the rare earth of suitable concentration has
The biological effect of environment resistance, rare earth also achieve significant social benefit and economic effect in popularization and application agriculturally
Benefit.Lanthanum (La) is one of metal most active in rare earth metal, can effectively adjust plant roots orga- nogenesis, seed is promoted to sprout
With raising photosynthesis of plant, anti-adversity and the crop yield of crop are improved.Its main application mode is root application and blade face
Spray two kinds.La3+(116pm) and Cd2+(109pm) has similar ionic radius, and the charge of lanthanum ion is higher than Cd ion.By
Be equal to the ratio between ionic charge and ionic radius in ionic potential, so the ionic potential of lanthanum ion is greater than Cd ion, this may make lanthanum from
Son is easier to be adsorbed to root system of plant surface, prevents Cd from being absorbed by plants by way of competition.In addition, plant absorption Cd master
To enter cell by specific transporters on plasma membrane.Nramp5(natural resistance-associated
Macrophage protein) it is located on plant roots plasma membrane, in rice and big wheat, Nramp5 gene silencing or knockout,
Overground part and underground part Cd content significantly reduce.It is thin that HMA4 (heavy metal ATPase4) is located at plant root pericycle
On cytoplasmic membrane, Cd is pumped out from cell and is come, overground part is transported to by the load of xylem.HMA4 gene silencing knocks out energy
Plant root Cd is enough effectively reduced to transport to overground part.It, can be effective while La is as cadmium agent reduction crop is subtracted to Cd accumulation
Promote crop yield.It therefore, will be one of the effective ways for solving agricultural Cd contaminated soil grain security hidden danger at present using lanthanum.
This also provides the new concept new technology administered for research and development heavy-metal contaminated soil to reference.
Summary of the invention
In order to reduce absorption and transhipment of the crop to Cd, the content of Cd in crop kernel is reduced, the present invention provides a kind of lanthanum
As the agent of crop cut cadmium and cadmium transporter gene expression inhibiting agent and the application of increasing agent.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of method of cadmium content in reduction crop kernel, which is wheat, in wheat growth to the jointing stage, Xiang Ge
Apply the reagent containing lanthanum ion in contaminated soil；Again at wheat growth to heading stage, examination of the foliage-spray containing lanthanum ion is carried out
Agent.
The method of cadmium content in a kind of above-mentioned reduction crop kernel carries out wheat when wheat growth to the jointing stage
Root applies lanthanum, and sowing amount is 1.8-2.2mg/kg lanthanum (by 1.13g/cm in the present embodiment3The soil weight, per acre soil quality
About 15.1 ten thousand kilograms calculating, i.e., apply lanthanum 272-332g/ mus into soil).Again at wheat growth to heading stage, to wheat
Foliage-spray lanthanum is carried out, concentration 15-25mg/L sprays 45-75L per acre.
The method of cadmium content, the lanthanum ion are administered in the form of lanthanum nitrate in a kind of above-mentioned reduction crop kernel.
Wherein 272-332g lanthanum is the amount rather than the amount of lanthanum nitrate of lanthanum element in lanthanum nitrate.
Meanwhile the reagent for containing lanthanum ion is also used as crop yield agent application:
A method of promoting crop yield, which is wheat, and this method is to apply in wheat root containing lanthanum ion
Reagent；Again at wheat growth to heading stage, reagent of the foliage-spray containing lanthanum ion is carried out.
A kind of method of above-mentioned promotion crop yield carries out root application to wheat when wheat growth to the jointing stage
Lanthanum, sowing amount are 1.8-2.2mg/kg lanthanum (by 1.13g/cm in the present embodiment3The soil weight, per acre soil quality are about 15.1
Ten thousand kilograms of calculating, i.e., apply lanthanum 272-332g/ mus into soil).Again at wheat growth to heading stage, blade face is carried out to wheat
Lanthanum is sprayed, concentration 15-25mg/L sprays 45-75L per acre.
A kind of method of above-mentioned promotion crop yield, the lanthanum ion are administered in the form of lanthanum nitrate.Wherein 272-
332g lanthanum is the amount rather than the amount of lanthanum nitrate of lanthanum element in lanthanum nitrate.
In addition, the reagent for containing lanthanum ion is also used as lowering crop to Cd absorption and transports related transporter gene
The inhibitor of expression:
A kind of inhibitor lowered crop and the expression of related transporter gene is absorbed and transported to Cd contains in the inhibitor
Lanthanum ion.The crop is wheat, in wheat growth to the jointing stage, applies the reagent containing lanthanum ion into cadmium pollution soil.
A kind of above-mentioned downward crop absorbs to Cd and transports the inhibitor of related transporter gene expression, the lanthanum from
Son is from lanthanum nitrate.
A kind of above-mentioned downward crop absorbs to Cd and transports the inhibitor of related transporter gene expression, the inhibitor
Method of application be root apply, when wheat growth to the jointing stage, to wheat carry out root apply lanthanum, sowing amount 1.8-
2.2mg/kg lanthanum is (by 1.13g/cm in the present embodiment3The soil weight, per acre soil quality be about 15.1 ten thousand kilograms calculating, i.e., to
Lanthanum 272-332g/ mus is applied in soil).
Further, the spraying method of the blade face lanthanum is that lanthanum nitrate is first dissolved in suitable water, and lanthanum aqueous solution is made,
Lanthanum aqueous solution is sprayed in wheat blade face at the cloudy day with sprayer again.Blade face is sprayed in the form of lanthanum nitrate.
Further, the method that the root applies lanthanum is that lanthanum nitrate is first dissolved in suitable water, according to applying for lanthanum nitrate
The lanthanum solution that dosage is scaled respective volume is manured into soil, and is manured into soil in the form of lanthanum nitrate.
The present invention applies lanthanum into soil by wheat time of infertility pot experiment to inhibit absorption of the wheat root to Cd
And transhipment；And resistance control further is carried out to Cd by way of foliage-spray lanthanum, make by wheat absorb Cd as few as possible to
It is shifted in wheat seed, to reduce content of the Cd in wheat seed.
The beneficial effects of the present invention are: the reagent of the invention containing lanthanum ion, can reduce crop kernel as cadmium agent is subtracted
The content of middle cadmium, and can be used as increasing agent, makes crop yield, at the same the reagent containing lanthanum ion Cd can also be absorbed as crop and
Transport the inhibitor of related transporter gene expression.
Detailed description of the invention
The present invention will be further explained below with reference to the attached drawings and specific examples.
Fig. 1 is influence of the lanthanum of the present invention to different time wheat root TaNramp5 gene relative expression.
Fig. 2 is influence of the lanthanum of the present invention to different time wheat root TaHMA4 gene relative expression.
Specific embodiment
Applicant will in conjunction with specific embodiments further illustrate the method for the present invention below.It should be understood that following embodiment
It is only used for making clear explanation to the claimed technical solution of the present invention, and is understood not to that the present invention is claimed
The limitation of range.Below with reference to subordinate list and embodiment, the present invention is further described.
Wheat seed used is Yao wheat 16 in following embodiments, accumulates wheat breed for low Cd, seed is by Jiangxi Province's agriculture
The industry academy of sciences provides.
Embodiment 1: root applies lanthanum
This example provides a kind of method for applying Cd content in lanthanum reduction wheat seed by root.Extremely to wheat growth
When the jointing stage, lanthanum is applied in the Cd contaminated soil of wheat growth, La is by inhibiting wheat root to absorb turn related to transhipment Cd
The expression of protein gene is transported, absorption and transhipment of the wheat root to Cd are reduced, to reduce content of the Cd in wheat seed.
20cm topsoil is acquired in Wuhan University campus nursery.After soil is brought back laboratory, visible stone is rejected
Block and Litter-fall, room-dry.The background value of soil Cd is 0.42mg/kg.Cd pollution is prepared in the form of external source adds Cd
Soil uses the Cd contaminated soil as trying soil for wheat cultivation.Selecting urea for nitrogenous fertilizer, calcium superphosphate is phosphate fertilizer, sulfuric acid
Potassium is potash fertilizer, and the amount of application of N P and K is respectively that the effective quantity of N is 100mg/kg, and the effective quantity of P is 80mg/kg, the effective quantity of K
For 50mg/kg.3 repetitions of every group of test.
Specific experiment step:
(1) test soil is brought back laboratory, and natural air drying rejects visible stone and Litter-fall, after grinding, crosses 2mm's
Sieve.10mg/kg Cd is added in the soil, after being thoroughly mixed, cures 1 month.
(2) apply 33.3% urea into soil obtained by (1), 100% calcium superphosphate and 100% potassium sulfate the bottom of as
Fertilizer.Remaining urea applies 33.3% in tillering stage and jointing stage respectively.
(3) soil 7.5kg obtained by (2) is packed into polypots (diameter 26.5cm, high 17.6cm), and in mid-October will be small
Wheat Seeds are seeded in polypots soil.
(4) outdoor cultivation is carried out to sample obtained by (3) according to wheat cultivation conventional method, to wheat growth to jointing stage
When, 2mg/kg lanthanum is added into polypots soil.
(5) after wheat is mature, harvest wheat entirety plant, Plant samples are cleaned up with tap water, and by its root,
Stem, leaf, glume, seed separation, are placed in baking oven, 65 DEG C drying to constant weight.
(6) dry constant weight sample obtained by (5) is put into plant disintegrating machine and is crushed, powder sample is placed in clean beaker
In, and appropriate mixing digestion solution (nitric acid and perchloric acid volume ratio=4:1) is added into beaker, it stands overnight.
(7) sample obtained by (6) is placed in electric hot plate heating to clear up, after constant volume, is surveyed with Atomic Absorption Spectrometer (TAS-990)
Determine the content of Cd in sample solution.
It can be fluctuated in a certain range in the present embodiment to the lanthanum that polypots adds, specifically, to wheat growth to jointing
When the phase, 1.8-2.2mg/kg lanthanum is added into polypots soil (by 1.13g/cm in the present embodiment3The soil weight, per acre soil
Quality is about 15.1 ten thousand kilograms of calculating, i.e., lanthanum 272-332g/ mus is applied into soil).
Embodiment 2: foliage-spray lanthanum
Present embodiments provide a kind of method for reducing Cd content in wheat seed with foliage-spray lanthanum.Extremely to wheat growth
Boot stage sprays blade face lanthanum, is transported by resistance control wheat aerial part Cd to seed, to reduce Cd containing in wheat seed
Amount.
20cm topsoil is acquired in Wuhan University campus nursery, after soil is brought back laboratory, rejects visible stone
Block and Litter-fall, room-dry.The background value of soil Cd is 0.42mg/kg.Cd pollution is prepared in the form of external source adds Cd
Soil uses the Cd contaminated soil as trying soil for wheat cultivation.Selecting urea for nitrogenous fertilizer, calcium superphosphate is phosphate fertilizer, nitrogen phosphorus
The amount of application of potassium is respectively that the effective quantity of N is 100mg/kg, and the effective quantity of P is 80mg/kg, and the effective quantity of K is 50mg/kg.Often
Group 3 repetitions of test.
Specific experiment step:
(1) test soil is brought back laboratory, and natural air drying rejects bulk stone and Litter-fall, after grinding, crosses 2mm's
Sieve.10mg/kg Cd is added in the soil, after being thoroughly mixed, cures 1 month.
(2) apply 33.3% urea into soil obtained by (1), 100% calcium superphosphate and 100% potassium sulfate the bottom of as
Fertilizer.Remaining urea applies 33.3% in tillering stage and jointing stage respectively.
(3) soil 7.5kg obtained by (2) is packed into polypots (diameter 26.5cm, high 17.6cm), and in mid-October will be small
Wheat Seeds are seeded in polypots soil.
(4) outdoor cultivation is carried out to sample obtained by (3) according to wheat cultivation conventional method, to wheat growth to boot stage
When, foliage-spray 20mg/L lanthanum is carried out to wheat, every alms bowl about sprays 5mL.
(5) after wheat is mature, harvest wheat entirety plant, Plant samples are cleaned up with tap water, and by its root,
Stem, leaf, glume, seed separation, are placed in baking oven, 65 DEG C drying to constant weight.
(6) dry constant weight sample obtained by (5) is put into plant disintegrating machine and is crushed, powder sample is placed in clean beaker
In, and appropriate mixing digestion solution (nitric acid and perchloric acid volume ratio=4:1) is added into beaker, it stands overnight.
(7) sample obtained by (6) is placed in electric hot plate heating to clear up, after constant volume, is surveyed with Atomic Absorption Spectrometer (TAS-990)
Determine the content of Cd in sample solution.
In the present embodiment, at wheat growth to heading stage, foliage-spray lanthanum carried out to wheat, concentration and the amount of spraying can be
It floats in a certain range, specially 15-25mg/L sprays 45-75L per acre.
Embodiment 3: root applies lanthanum and foliage-spray lanthanum joint carries out
It present embodiments provides one kind and lanthanum is applied by root, and the mode for further combining foliage-spray lanthanum reduces wheat
The method of Cd content in seed.Root applies lanthanum when wheat growth to the jointing stage, and lanthanum is by inhibiting wheat root to absorb and turn
The expression of Cd correlation transporter gene is transported, absorption and transhipment of the wheat root to Cd are reduced.It is sprayed again on wheat heading stage blade face
Lanthanum is applied, is transported by resistance control wheat aerial part Cd to seed, is further reduced the content of Cd in wheat seed.
20cm topsoil is acquired in Wuhan University campus nursery, after soil is brought back laboratory, rejects visible stone
Block and Litter-fall, room-dry.The background value of soil Cd is 0.42mg/kg.Cd pollution is prepared in the form of external source adds Cd
Soil uses the Cd contaminated soil as trying soil for wheat cultivation.Selecting urea for nitrogenous fertilizer, calcium superphosphate is phosphate fertilizer, sulfuric acid
Potassium is potash fertilizer, and the amount of application of N P and K is respectively that the effective quantity of N is 100mg/kg, and the effective quantity of P is 80mg/kg, the effective quantity of K
For 50mg/kg.3 repetitions of every group of test.
Specific experiment step:
(1) test soil is brought back laboratory, and natural air drying rejects visible stone and Litter-fall, after grinding, crosses 2mm's
Sieve.10mg/kg Cd is added in the soil, after being thoroughly mixed, cures 1 month.
(2) apply 33.3% urea into soil obtained by (1), 100% calcium superphosphate and 100% potassium sulfate the bottom of as
Fertilizer.Remaining urea applies 33.3% in tillering stage and jointing stage respectively.
(3) soil 7.5kg obtained by (2) is packed into polypots (diameter 26.5cm, high 17.6cm), and in mid-October will be small
Wheat Seeds are seeded in polypots soil.
(4) outdoor cultivation is carried out to sample obtained by (3) according to wheat cultivation conventional method, to phase wheat growth jointing stage
When, apply 2mg/kg lanthanum into polypots soil.Foliage-spray 20mg/L is carried out when wheat growth to boot stage, then to wheat
Lanthanum, every alms bowl about spray 5mL.
(5) after wheat is mature, harvest wheat entirety plant, Plant samples are cleaned up with tap water, and by its root,
Stem, leaf, glume, seed separation, are placed in baking oven, 65 DEG C drying to constant weight.
(6) dry constant weight sample obtained by (5) is put into plant disintegrating machine and is crushed, powder sample is placed in clean beaker
In, and appropriate mixing digestion solution (nitric acid and perchloric acid volume ratio=4:1) is added into beaker, it stands overnight.
(7) sample obtained by (6) is placed in electric hot plate heating to clear up, after constant volume, is surveyed with Atomic Absorption Spectrometer (TAS-990)
Determine the content of Cd in sample solution.
It can be fluctuated in a certain range in the present embodiment to the lanthanum that polypots adds, specifically, to wheat growth to jointing
When the phase, 1.8-2.2mg/kg lanthanum is added into polypots soil (by 1.13g/cm in the present embodiment3The soil weight, per acre soil
Quality is about 15.1 ten thousand kilograms of calculating, i.e., lanthanum 272-332g/ mus is applied into soil).At wheat growth to heading stage, to small
Wheat carries out foliage-spray lanthanum, and concentration and the amount of spraying can float in a certain range, and specially 15-25mg/L sprays 45- per acre
75L。
Comparative example 1
20cm topsoil is acquired in Wuhan University campus nursery, after soil is brought back laboratory, rejects visible stone
Block and Litter-fall, room-dry.The background value of soil Cd is 0.42mg/kg.Cd pollution is prepared in the form of external source adds Cd
Soil uses the Cd contaminated soil as trying soil for wheat cultivation.Selecting urea for nitrogenous fertilizer, calcium superphosphate is phosphate fertilizer, sulfuric acid
Potassium is potash fertilizer, and the amount of application of N P and K is respectively that the effective quantity of N is 100mg/kg, and the effective quantity of P is 80mg/kg, the effective quantity of K
For 50mg/kg.3 repetitions of every group of test.
Specific experiment step:
(1) test soil is brought back laboratory, and natural air drying rejects visible stone and Litter-fall, after grinding, crosses 2mm's
Sieve.10mg/kg Cd is added in the soil, after being thoroughly mixed, cures 1 month.
(2) apply 33.3% urea into soil obtained by (1), 100% calcium superphosphate and 100% potassium sulfate the bottom of as
Fertilizer.Remaining urea applies 33.3% in tillering stage and jointing stage respectively.
(3) soil 7.5kg obtained by (2) is packed into polypots (diameter 26.5cm, high 17.6cm), and in mid-October will be small
Wheat Seeds are seeded in polypots soil.
(4) outdoor cultivation is carried out to sample obtained by (3) according to wheat conventional cultivation method.
(5) after wheat is mature, harvest wheat entirety plant, Plant samples are cleaned up with tap water, and by its root,
Stem, leaf, glume, seed separation, are placed in baking oven, 65 DEG C drying to constant weight.
(6) dry constant weight sample obtained by (5) is put into plant disintegrating machine and is crushed, powder sample is placed in clean beaker
In, and appropriate mixing digestion solution (nitric acid and perchloric acid volume ratio=4:1) is added into beaker, it stands overnight.
(7) sample obtained by (6) is placed in electric hot plate heating to clear up, after constant volume, is surveyed with Atomic Absorption Spectrometer (TAS-990)
Determine the content of Cd in sample solution.
1 test result of embodiment 1-3 and comparative example: by above-described embodiment 1-3 and comparative example 1, totally four groups of experiments carry out data
Collect and compare, as shown in table 1.According to result in table 1 as it can be seen that it is different implement processing wheat is absorbed and is transported Cd have it is obvious poor
It is different.Compared with comparative example 1, embodiment 1-3 reduces wheat root, stem, leaf, glume, Cd content in seed.Wherein embodiment 3
Middle wheat seed Cd content is minimum (only 2.14 ± 0.06mg/kg), reduces 36.9% compared with comparative example 1.Secondly, different implement
Processing increases wheat yield, and wherein embodiment 3 (7.72 ± 0.47) increases 10.4% compared with comparative example 1.Experimental result is said
It is bright, the content of Cd in wheat seed can be effectively reduced by the scheme of embodiment 3, while increasing yield.
Influence of the 1 lanthanum difference applying mode of table to wheat different parts Cd content and yield
Embodiment 4: water culture verifies inhibitor of the lanthanum as crop absorption and transhipment Cd protein gene expression
By water culture experiment, discovery lanthanum can effectively lower wheat and absorb the related transport protein base with transhipment Cd the present embodiment
The expression of cause.Specific test procedure is as follows:
(1) select full consistent appropriate wheat seed, 5% sodium hypochlorite impregnate 15min, after rinsed with deionized water
Completely.By the uniform laying of the wheat seed of wash clean into the culture dish for being lined with filter paper, and appropriate deionization is added on filter paper
Water keeps seed wet, is put into dark incubator and sprouts.Kept for 25 DEG C of temperature, humidity is preculture two under conditions of 80%
It allows seed to sprout.
(2) seed that (1) is sprouted is transferred in clean quartz sand, with the Huo Gelan Solution culture method of 1/2 intensity.Huo Ge
Blue nutrient solution contains: 2mM MgSO4·7H2O,5mM Ca(NO3)2·4H2O,5mM KNO3,1mM KH2PO4,0.02mM Fe-
EDTA,0.045mM H3BO3,0.91μM MnCl2,0.32μM CuSO4·5H2O, 0.76 μM of ZnSO4·7H2O and 0.038 μM
NaMoO4·2H2O.PH value is adjusted to 6.0 with NaOH or HCl.Plant is 26/20 DEG C, humidity in natural light, daytime/nocturnal temperature
It is 70/85%, photosynthetic photon flux is 400 μm of ol m-2s-1Under conditions of cultivate.
(3) when the wheat of (2) culture it is long to two leaves wholeheartedly when, select the consistent seedling of growing way at 1.5 liters containing lattice suddenly completely
Preculture 3 days in the plastic tub of blue nutrient solution.
(4) by the setting of wheat seedling obtained by (3) blank control (CK), 1mg/L lanthanum processing (La), 1mg/L Cd processing
(Cd), 1mg/L Cd+1mg/L lanthanum processing (Cd+La).Lanthanum ion and phosphate anion generate lanthanum orthophosphate precipitating in order to prevent, small
Wheat seedling is in removal KH2PO4It is handled in complete Huo Gelan nutrient solution, passes through foliage-spray 0.5mM KH2PO4Mode supplement it is small
P element needed for wheat growth.Wheat root sample is harvested after handling 12,24 and 72 hours respectively.
(5) (4) resulting sample is frozen in -80 DEG C of refrigerators.
(6) according to Trizol (Invitrogen) kit specification, sample RNA obtained by (5) is extracted, 1% agarose is solidifying
Gel electrophoresis and UV spectrophotometer measuring RNA mass and concentration.
(7) sample obtained by (6) is inverted using the PrimeScript reagent Kit kit of Takara company
Record becomes cDNA.
(8) it is searched according to Genebank accession number, the gene sequence of TaNramp5 and TaHMA4 and internal reference β-TaTubulin
Column, separately design the upstream and downstream primer of its quantitative fluorescent PCR.Its sequence number and primer are as shown in table 2.
(9) sample obtained by (7) is subjected to quantitative fluorescent PCR.Gene expression in relation to (8) uses SYBR Premix Ex
Taq (Takara) dyestuff, according to StepOneTMThe side of Real-Time PCR System (Applied Biosystems, USA)
Formula carries out.
(10) relative expression quantity of gene is normalized by the method for Δ Δ Ct with CFX Manager software.
2 Fluorescent Quantitative-PCR primer of table
Test result is as illustrated in fig. 1 and 2.The result shows that compared with the control group, Cd processing raised TaNramp5 and
The gene expression of TaHMA4.However, after adding lanthanum and Cadmium treated simultaneously, the expression quantity of TaNramp5 and TaHMA4 gene quilt
It lowers.Moreover, individually -72h, the expression quantity of TaNramp5 and TaHMA4 gene are also lowered for 24 hours after the processing of addition lanthanum.These knots
Fruit illustrates that lanthanum is a kind of inhibitor of Cd transporter gene expression.
Sequence table
<110>Wuhan University
<120>lanthanum is as the agent of crop cut cadmium and cadmium transporter gene expression inhibiting agent and the application of increasing agent
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Claims (9)
1. a kind of method for reducing cadmium content in crop kernel, which is characterized in that the crop is wheat, wheat growth to jointing
When the phase, apply the reagent containing lanthanum ion into soil；Again at wheat growth to heading stage, foliage-spray is carried out containing lanthanum ion
Reagent.
2. a kind of method for reducing cadmium content in crop kernel according to claim 1, which is characterized in that wheat growth
When to the jointing stage, 1.8-2.2mg/kg lanthanum is added into soil, then at wheat growth to heading stage, blade face spray is carried out to wheat
Lanthanum is applied, concentration 15-25mg/L sprays 45-75L per acre.
3. a kind of method for reducing cadmium content in crop kernel according to claim 1, which is characterized in that the lanthanum ion
It is administered in the form of lanthanum nitrate.
4. a kind of method for promoting crop yield, which is characterized in that the crop is wheat, in wheat growth to the jointing stage,
Apply the reagent containing lanthanum ion into soil；Again at wheat growth to heading stage, reagent of the foliage-spray containing lanthanum ion is carried out.
5. a kind of method for promoting crop yield according to claim 4, which is characterized in that wheat growth to jointing stage
When, 1.8-2.2mg/kg lanthanum is added into soil, then at wheat growth to heading stage, foliage-spray lanthanum is carried out to wheat, it is dense
Degree is 15-25mg/L, sprays 45-75L per acre.
6. a kind of method for promoting crop yield according to claim 4, which is characterized in that the lanthanum ion is with lanthanum nitrate
Form be administered.
7. a kind of inhibitor lowering crop and expressing Cd uptake transporter gene related with transhipment, which is characterized in that the suppression
Contain lanthanum ion in preparation.
8. a kind of inhibition lowering crop and expressing Cd uptake transporter gene related with transhipment according to claim 7
Agent, which is characterized in that the crop is wheat.
9. a kind of inhibition lowering crop and expressing Cd uptake transporter gene related with transhipment according to claim 7
Agent, which is characterized in that the method for application of the inhibitor is to apply from root, and sowing amount is 1.8-2.2mg/kg lanthanum.
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| CN114762487A (en) * | 2020-12-30 | 2022-07-19 | 广东地球土壤研究院 | Method for planting crops by using lanthanum chloride |
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