CN109280612A - Incubator is used in a kind of experiment of microbial reproduction - Google Patents
Incubator is used in a kind of experiment of microbial reproduction Download PDFInfo
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- CN109280612A CN109280612A CN201811259263.9A CN201811259263A CN109280612A CN 109280612 A CN109280612 A CN 109280612A CN 201811259263 A CN201811259263 A CN 201811259263A CN 109280612 A CN109280612 A CN 109280612A
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- circuit
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- ceramic liner
- equipment box
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to microbe research technical field and disclose a kind of microbial reproduction experiment incubator, including cabinet.The present invention passes through setting electric hot plate and semiconductor chilling plate, the control to ceramic liner internal temperature can be achieved, preferable temperature environment is provided for microbial reproduction experiment, by the way that ultraviolet germicidal lamp is arranged, antivirus processing can be carried out to ceramic liner inside before being put into culture medium, it can effectively avoid bacterium and culture microbe symbiotic inside ceramic liner, cause experimental data deviation, by the way that observation window is arranged, the observation window is electric-control color-changeable glass, it is clear to be powered, laboratory technician is facilitated to observe microculture situation, power-off is fuzzy, it can effectively avoid light to enter in ceramic liner, good living environment is provided for microorganism.
Description
Technical field
Present invention relates particularly to a kind of microbial reproduction experiment incubators, belong to microbe research technical field.
Background technique
In microculture experimentation, need using incubator is arrived, existing incubator needs individually before use
Sterilization processing is carried out to incubator inside, it is inconvenient, therefore, it is proposed that incubator is used in a kind of experiment of microbial reproduction.
Summary of the invention
The technical problem to be solved in the present invention overcomes existing defect, provides a kind of microbial reproduction experiment incubator,
By setting electric hot plate and semiconductor chilling plate, it can be achieved that the control to ceramic liner internal temperature, is tested for microbial reproduction
Preferable temperature environment is provided, by the way that ultraviolet germicidal lamp is arranged, ceramic liner inside can be killed before being put into culture medium
Poison processing can effectively avoid bacterium and culture microbe symbiotic inside ceramic liner, cause experimental data deviation, be seen by setting
Window is examined, which is electric-control color-changeable glass, and it is clear to be powered, and laboratory technician is facilitated to observe microculture situation, and power-off is fuzzy, can
Effectively light is avoided to enter in ceramic liner, provides good living environment for microorganism, can effectively solve background technique
The problems in.
In order to solve the above-mentioned technical problems, the present invention provides the following technical solutions:
The present invention provides a kind of microbial reproduction experiment incubator, including cabinet, the box house from top to bottom according to
It is secondary to be equipped with ceramic liner and equipment box, chamber door, the pottery are rotatably connected to by hinge on the cabinet on front side of the ceramic liner
It is provided with opening on front side of porcelain liner, is successively arranged several placement plates, the placement plate bottom inside the ceramic liner from top to bottom
Portion is fixedly installed electric hot plate, is successively fixedly installed ultraviolet sterilization from top to bottom on the inner wall on the right side of the ceramic liner
Lamp and temperature sensor, the ceramic liner outside is wound with cooling winding pipe, between the cooling winding pipe and cabinet wall
Successively be fixedly installed from left to right filled with shock absorbing foam pad, on the bottom plate inside the equipment box power supply, relay B, after
Electric appliance A, water tank and water pump are fixedly installed semiconductor chilling plate, the semiconductor chilling plate on inner wall on the right side of the equipment box
Including cold and hot end, inner wall is fixed on the right side of the semiconductor chilling plate hot end and equipment box, on the right side of the equipment box inner wall with
Semiconductor chilling plate hot end joint place is provided with heat release hole, is provided with observation window on the chamber door, in observation window on the chamber door
Bottom is provided with control panel, is successively arranged display screen and control button, the control plane on the control panel from left to right
It is successively arranged switch A and switch B from top to bottom on the right side of control button on plate.
Preferably, described cooling winding pipe one end, which is protruded into after equipment box is bonded with the cold end of semiconductor chilling plate, connects water
Pump, the cooling winding pipe other end are protruded into equipment box and are connect with water tank, and the water pump is connected by water pipe and water tank, described
Water pump is electrically connected by circuit and relay A, and the semiconductor chilling plate is electrically connected by circuit and relay A, described
Relay A is electrically connected with the power supply by circuit.
Preferably, the electric hot plate is electrically connected by circuit and relay B, and the relay B passes through circuit and power supply
It is electrically connected.
Preferably, the ultraviolet germicidal lamp is electrically connected respectively at switch A and power supply in series by circuit,
The observation window is electric-control color-changeable glass, and the observation window is electrical respectively at switch B and power supply in series by circuit
Connection.
Preferably, controller is fixedly installed inside the control panel, the temperature sensor passes through circuit and control
Device is electrically connected, and the power supply is electrically connected by circuit and controller, the relay A and relay B pass through circuit with
Controller is electrically connected, and the display screen and control button pass through circuit and controller electric connection.
The beneficial effects obtained by the present invention are as follows being:
(1) by setting controller, which is single-chip microcontroller, at model ARM Cortex-M3 STM32F103
Device chip is managed, the processing chip is with high-performance flash memory module, SRAM and 4GB addressable memory space built in 20K byte, separately
Outer ARM Cortex-M3STM32F103 processor chips provide stable 5V/3.3V power input, by control button to place
After managing device input temperature values, processor can be by temperature sensor real-time monitoring ceramic liner internal temperature, when temperature value is higher than
When setting value, processor controls relay B open circuit, and relay A access makes electric hot plate be in off-position, relay A access
When, semiconductor chilling plate and water pump are run simultaneously, and water pump is oriented to the system of semiconductor chilling plate after extracting the coolant liquid in water tank out
Cold end, the coolant liquid after being cooled flow to outside ceramic liner, the temperature of ceramic liner can be reduced, when ceramic liner internal temperature
When lower than setting value, processor controls relay B access, and relay A open circuit makes electric hot plate be powered and runs, will be ceramic with realization
Liner internal temperature control provides preferable environment in the state of relative constant, for microorganism.
(2) by the way that ultraviolet germicidal lamp is arranged on ceramic liner inner wall, before placing culture medium toward on rack, pass through
Switch A opens ultraviolet germicidal lamp, first carries out antivirus processing to ceramic liner inside, can effectively avoid bacterium inside ceramic liner
With culture microbe symbiotic, experimental data deviation is caused, by the way that observation window is arranged, which is electric-control color-changeable glass, is powered
Clearly, laboratory technician is facilitated to observe microculture situation, power-off is fuzzy, can effectively avoid light and enters in ceramic liner.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.
In the accompanying drawings:
Fig. 1 is a kind of microbial reproduction experiment incubator overall structure diagram described in the embodiment of the present invention；
Fig. 2 is a kind of microbial reproduction experiment incubator cross section structure schematic diagram described in the embodiment of the present invention；
Fig. 3 is a kind of microbial reproduction experiment incubator chamber door front side structure schematic diagram described in the embodiment of the present invention；
Figure label: 1, cabinet；2, chamber door；3, equipment box；4, ceramic liner；5, placement plate；6, observation window；7, temperature
Sensor；8, heat release hole；9, ultraviolet germicidal lamp；10, electric hot plate；11, shock absorbing foam pad；12, cooling winding pipe；13, it partly leads
Body cooling piece；14, water pump；15, water tank；16, relay A；17, relay B；18, power supply；19, control panel；20, display screen；
21, control button；22, switch A；23, switch B.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment: Fig. 1-3, the present invention a kind of microbial reproduction experiment incubator, including cabinet 1, cabinet 1 are please referred to
Inside is successively arranged ceramic liner 4 and equipment box 3 from top to bottom, is rotatablely connected on the cabinet 1 of 4 front side of ceramic liner by hinge
There is chamber door 2, opening is provided on front side of ceramic liner 4, several placement plates 5 is successively arranged inside ceramic liner 4 from top to bottom, puts
It sets 5 bottom of plate and is fixedly installed electric hot plate 10, be successively fixedly installed from top to bottom on the inner wall on 4 right side of ceramic liner ultraviolet
Line bactericidal lamp 9 and temperature sensor 7, are wound with cooling winding pipe 12 on the outside of ceramic liner 4, cooling winding pipe 12 in cabinet 1
It is filled with shock absorbing foam pad 11 between wall, is successively fixedly installed power supply 18, relay from left to right on the bottom plate inside equipment box 3
Device B17, relay A16, water tank 15 and water pump 14 are fixedly installed semiconductor chilling plate 13 on 3 right side inner wall of equipment box, partly lead
Body cooling piece 13 includes cold and hot end, and 13 hot end of semiconductor chilling plate is fixed with 3 right side inner wall of equipment box, 3 right side of equipment box
Inner wall and 13 hot end joint place of semiconductor chilling plate are provided with heat release hole 8, are provided with observation window 6 on chamber door 2, in sight on chamber door 2
It examines 6 bottom of window and is provided with control panel 19, be successively arranged display screen 20 and control button 21 from left to right on control panel 19,
It is successively arranged switch A22 and switch B23 from top to bottom in 21 right side of control button on control panel 19.
Further, cooling 12 one end of winding pipe, which is protruded into after equipment box 3 is bonded with the cold end of semiconductor chilling plate 13, connects water
Pump 14, cooling 12 other end of winding pipe protrudes into equipment box 3 to be connect with water tank 15, and water pump 14 is connected by water pipe and water tank 15,
Water pump 14 is electrically connected by circuit and relay A16, and semiconductor chilling plate 13 is electrically connected by circuit and relay A16,
Relay A16 is electrically connected by circuit and power supply 18, and electric hot plate 10 is electrically connected by circuit and relay B17, relay
B17 is electrically connected by circuit and power supply 18, ultraviolet germicidal lamp 9 by circuit in series respectively at switch A22 with
Power supply 18 is electrically connected, and observation window 6 is electric-control color-changeable glass, and observation window 6 is by circuit in series respectively at switch B23
It is electrically connected with power supply 18, controller is fixedly installed inside control panel 19, temperature sensor 7 passes through circuit and controller electricity
Property connection, power supply is electrically connected by circuit and controller, and relay A16 and relay B17 pass through circuit and controller electricity
Property connection, display screen 20 and control button 21 are electrically connected by circuit and controller.
Further, by the way that controller is arranged, which is single-chip microcontroller, model ARM Cortex-M3
STM32F103 processor chips, the processing chip have high-performance flash memory module, and SRAM and 4GB built in 20K byte may have access to
Memory space, in addition ARM Cortex-M3STM32F103 processor chips provide stable 5V/3.3V power input, pass through control
Button processed to after processor input temperature values, processor can by temperature sensor real-time monitoring ceramic liner internal temperature, when
When temperature value is higher than setting value, processor controls relay B open circuit, and relay A access makes electric hot plate be in off-position, after
When electric appliance A access, semiconductor chilling plate and water pump are run simultaneously, and water pump is oriented to semiconductor system after extracting the coolant liquid in water tank out
Cold refrigeration end, the coolant liquid after being cooled flow to outside ceramic liner, can reduce the temperature of ceramic liner, work as ceramic liner
When internal temperature is lower than setting value, processor controls relay B access, and relay A open circuit makes electric hot plate be powered and runs, with reality
Now by ceramic liner internal temperature control in the state of relative constant, preferable environment is provided for microorganism.
Further, by the way that ultraviolet germicidal lamp is arranged on ceramic liner inner wall, before placing culture medium toward on rack,
Ultraviolet germicidal lamp is opened by switch A, antivirus processing first is carried out to ceramic liner inside, can effectively avoid inside ceramic liner
Bacterium and culture microbe symbiotic, cause experimental data deviation, and by the way that observation window is arranged, which is electric-control color-changeable glass,
It is clear to be powered, and laboratory technician is facilitated to observe microculture situation, and power-off is fuzzy, can effectively avoid light and enters ceramic liner
It is interior.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (5)
1. a kind of microbial reproduction experiment incubator, including cabinet (1), it is characterised in that: the cabinet (1) is internal from upper past
Under be successively arranged ceramic liner (4) and equipment box (3), pass through hinge rotation on the cabinet (1) on front side of the ceramic liner (4) and connect
It is connected to chamber door (2), opening is provided on front side of the ceramic liner (4), is successively arranged from top to bottom inside the ceramic liner (4)
Several placement plates (5), placement plate (5) bottom is fixedly installed electric hot plate (10), interior on the right side of the ceramic liner (4)
Ultraviolet germicidal lamp (9) and temperature sensor (7), ceramic liner (4) outside are successively fixedly installed on wall from top to bottom
It is wound with cooling winding pipe (12), is filled with shock absorbing foam pad (11) between the cooling winding pipe (12) and cabinet (1) inner wall,
It is successively fixedly installed power supply (18), relay B (17), relay A from left to right on the internal bottom plate of the equipment box (3)
(16), water tank (15) and water pump (14) are fixedly installed semiconductor chilling plate (13), institute on inner wall on the right side of the equipment box (3)
Stating semiconductor chilling plate (13) includes cold and hot end, inner wall on the right side of semiconductor chilling plate (13) hot end and equipment box (3)
Fixed, inner wall and semiconductor chilling plate (13) hot end joint place are provided with heat release hole (8), the case on the right side of the equipment box (3)
It is provided with observation window (6) on door (2), observation window (6) bottom is provided with control panel (19), the control on the chamber door (2)
Display screen (20) and control button (21) are successively arranged on panel (19) processed from left to right, in control on the control panel (19)
It is successively arranged switch A (22) and switch B (23) from top to bottom on the right side of button (21).
2. a kind of microbial reproduction experiment incubator according to claim 1, it is characterised in that: the cooling winding pipe
(12) connection water pump (14) after equipment box (3) is bonded with the cold end of semiconductor chilling plate (13), the cooling winding are protruded into one end
Pipe (12) other end protrudes into equipment box (3) to be connect with water tank (15), and the water pump (14) is connected by water pipe and water tank (15),
The water pump (14) is electrically connected by circuit and relay A (16), and the semiconductor chilling plate (13) passes through circuit and relay
Device A (16) is electrically connected, and the relay A (16) is electrically connected by circuit and power supply (18).
3. a kind of microbial reproduction experiment incubator according to claim 1, it is characterised in that: the electric hot plate (10)
It is electrically connected by circuit and relay B (17), the relay B (17) is electrically connected by circuit and power supply (18).
4. a kind of microbial reproduction experiment incubator according to claim 1, it is characterised in that: the ultraviolet sterilization
Lamp (9) is electrically connected respectively at switch A (22) and power supply (18) in series by circuit, and the observation window (6) is electricity
Photo chromic glass is controlled, the observation window (6) is electrically connected respectively at switch B (23) and power supply (18) in series by circuit
It connects.
5. a kind of microbial reproduction experiment incubator according to claim 1, it is characterised in that: the control panel
(19) internal to be fixedly installed controller, the temperature sensor (7) is electrically connected by circuit and controller, and the power supply is logical
Oversampling circuit and controller are electrically connected, and the relay A (16) and relay B (17) are electrically connected by circuit with controller
It connects, the display screen (20) and control button (21) are electrically connected by circuit and controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811259263.9A CN109280612A (en) | 2018-10-26 | 2018-10-26 | Incubator is used in a kind of experiment of microbial reproduction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811259263.9A CN109280612A (en) | 2018-10-26 | 2018-10-26 | Incubator is used in a kind of experiment of microbial reproduction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109280612A true CN109280612A (en) | 2019-01-29 |
Family
ID=65177493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811259263.9A Withdrawn CN109280612A (en) | 2018-10-26 | 2018-10-26 | Incubator is used in a kind of experiment of microbial reproduction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109280612A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114217648A (en) * | 2021-11-30 | 2022-03-22 | 盐城市正龙电热科技有限公司 | Intelligent temperature control device with high cooling efficiency |
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| CN2152805Y (en) * | 1993-03-27 | 1994-01-12 | 杭州解放电子仪器厂 | Adjustable thermostated container |
| US20150136356A1 (en) * | 2013-11-20 | 2015-05-21 | Yao Hong Precision Technology Co., Ltd. | Machine for drinking water to be heated/cooled instantly |
| CN204522988U (en) * | 2015-02-28 | 2015-08-05 | 山东省农作物种质资源中心 | Semiconductor refrigerating incubator |
| CN204702759U (en) * | 2015-06-08 | 2015-10-14 | 周赞虎 | Microbiological incubator |
| CN206533868U (en) * | 2017-02-23 | 2017-10-03 | 山东建筑大学 | A kind of intelligent dimming bean sprout growing machine |
| WO2018086537A1 (en) * | 2016-11-10 | 2018-05-17 | 宁波华仪宁创智能科技有限公司 | Cell incubator and operating method thereof |
| CN207376058U (en) * | 2017-06-07 | 2018-05-18 | 广州市源起生物科技有限公司 | A kind of sterilized CO2gas incubator |
-
2018
- 2018-10-26 CN CN201811259263.9A patent/CN109280612A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2152805Y (en) * | 1993-03-27 | 1994-01-12 | 杭州解放电子仪器厂 | Adjustable thermostated container |
| US20150136356A1 (en) * | 2013-11-20 | 2015-05-21 | Yao Hong Precision Technology Co., Ltd. | Machine for drinking water to be heated/cooled instantly |
| CN204522988U (en) * | 2015-02-28 | 2015-08-05 | 山东省农作物种质资源中心 | Semiconductor refrigerating incubator |
| CN204702759U (en) * | 2015-06-08 | 2015-10-14 | 周赞虎 | Microbiological incubator |
| WO2018086537A1 (en) * | 2016-11-10 | 2018-05-17 | 宁波华仪宁创智能科技有限公司 | Cell incubator and operating method thereof |
| CN206533868U (en) * | 2017-02-23 | 2017-10-03 | 山东建筑大学 | A kind of intelligent dimming bean sprout growing machine |
| CN207376058U (en) * | 2017-06-07 | 2018-05-18 | 广州市源起生物科技有限公司 | A kind of sterilized CO2gas incubator |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114217648A (en) * | 2021-11-30 | 2022-03-22 | 盐城市正龙电热科技有限公司 | Intelligent temperature control device with high cooling efficiency |
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Application publication date: 20190129 |
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| WW01 | Invention patent application withdrawn after publication |