CN109280081A - A method of sialoglycopeptide is prepared from tuna ovum - Google Patents
A method of sialoglycopeptide is prepared from tuna ovum Download PDFInfo
- Publication number
- CN109280081A CN109280081A CN201811260150.0A CN201811260150A CN109280081A CN 109280081 A CN109280081 A CN 109280081A CN 201811260150 A CN201811260150 A CN 201811260150A CN 109280081 A CN109280081 A CN 109280081A
- Authority
- CN
- China
- Prior art keywords
- sialoglycopeptide
- tuna
- egg
- degreasing
- fish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of methods that sialoglycopeptide is prepared from tuna ovum, compensate for blank of China's tuna secondary industry in terms of sialoglycopeptide.This method is to obtain tuna ovum sialoglycopeptide crude product through ultrasonic wave auxiliary water extraction method using degreasing fish-egg, and after -50 post separation of sephadex G and activated carbon adsorption, tuna ovum sialoglycopeptide sterling is obtained after being eluted using methanol-water solution.The preparation method was both simple and convenient, and recovery rate is higher, and improved the safety of product.
Description
Technical field
The present invention relates to food processing technology fields, and in particular to a kind of side that sialoglycopeptide is prepared from tuna ovum
Method.
Background technique
Sialoglycopeptide is a kind of N sugar chain glycopeptide of complexity, can only separate obtain from natural products at present.Sialic acid sugar
Peptide is a kind of glycopeptide of humanization, has the function of adjusting the permeability of glomerulus, inhibits osteoclast development.The saliva of high activity
Liquid acid glycopeptide is primarily present in sexually matured animal egg cell.Isolate and purify in the world sialoglycopeptide method mainly with
Based on the method that Japanese Akira Seko was reported in 1997.This method will be by the separation of sephadex twice and a DEAE
Ion exchange column and a CM-SephadexC separation, process is extremely complex, and the period is longer.Certain domestic patent is to utilize a Portugal
Polysaccharide gel is separated, after active carbon adsorption using acetonitrile elution method obtain egg yolk sialoglycopeptide, process simplification,
Yield is higher, but since extraction process introduces phenol, the process of isolating and purifying introduces acetonitrile, so that the sialoglycopeptide obtained
There are serious security risks.
Tuna is the deep-sea economic fish of high amount of fishing, be the big nutrition fish in the world three recommended of international Nutrition Society it
One.After measured, it is 4% or so that the content of sialoglycopeptide, which accounts for dry weight percentage, in mature tuna ovum.Currently, there is no
Any document reports tuna ovum sialoglycopeptide.In view of the high bioactivity of sialoglycopeptide, develop a kind of from gold
The preparation method that sialoglycopeptide is obtained in marlin ovum develops the added value for increasing tuna and the health industry for promoting the mankind
With active influence.
Summary of the invention
Object of the present invention is to: a kind of method that sialoglycopeptide is prepared from tuna ovum is provided, to solve the above problems.
The technical scheme is that
A method of sialoglycopeptide being prepared from tuna ovum, this method comprises the following steps:
(1) it thaws after taking out the tuna fish-egg of freezing, be freeze-dried after homogenate, obtain tuna fish-egg powder；
(2) the tuna fish-egg powder is filtered after dehydrated alcohol degreasing and is dried, obtain degreasing tuna fish-egg powder；
(3) the degreasing tuna fish-egg powder is assisted into lower extraction, centrifugation, concentration in ultrasonic wave through deionized water, obtained
Concentrate；
(4) concentrate is concentrated under reduced pressure after -50 post separation of sephadex G, obtains tuna fish-egg sialic acid
Glycopeptide crude product；
(5) the tuna fish-egg sialoglycopeptide crude product is utilized into activated carbon adsorption, is washed through methanol-water solution
It is de-, obtain tuna fish-egg sialoglycopeptide sterling.
Further, the temperature of defrosting described in step (1) is 4 DEG C, and the temperature of the freezing is -20 DEG C, and the time is
12h。
Further, the tuna fish-egg powder is filtered described in step (2) after dehydrated alcohol degreasing and is dried, obtained
Degreasing tuna fish-egg powder includes: that stirring 10h in the dehydrated alcohol of 10-15 times of volume is added in the tuna fish-egg powder to carry out
Degreasing, during which every 3h replaces a dehydrated alcohol, filters rouge by bilayer after degreasing and is filtered to remove ethyl alcohol, takes filter residue in cold air drying
6-10h is air-dried in machine obtains degreasing tuna fish-egg powder.
Further, described in step (3) by the degreasing tuna fish-egg powder through deionized water in ultrasonic wave auxiliary under
Extract, centrifugation, concentration, obtain concentrate include: by the degreasing tuna fish-egg powder by its w/v 20-30 extraordinarily
Enter to carry out the extraction of sialoglycopeptide in deionized water, carries out ultrasonication per hour during milking to assist obtaining saliva
Sour glycopeptide is centrifuged after acquisition, and supernatant is taken to be concentrated under reduced pressure at 40-45 DEG C, obtains concentrate.
Further, the Extracting temperature is 4-10 DEG C, extraction time 12-16h, and the ultrasonic treatment time is
10-30min, ultrasonic sound field intensity are 200~300W/cm2, supersonic frequency 30kHz, centrifugal rotational speed 8000r/min, centrifugation
Time is 10-15min.
Further, the concentrate is concentrated under reduced pressure described in step (4) after -50 post separation of sephadex G, is obtained
It include: to separate the concentrate through -50 column of sephadex G to tuna fish-egg sialoglycopeptide crude product, temperature is
20-30 DEG C, flow velocity 1.0-1.5ml/min, every 7min collection eluent judges the saliva in eluent with high performance liquid chromatography
It is de- whether liquid acid glycopeptide flows, and collects the eluent containing sialoglycopeptide and is concentrated under reduced pressure at 40-45 DEG C, is freeze-dried,
Obtain tuna fish-egg sialoglycopeptide crude product.
Further, activated carbon adsorption described in step (5) are as follows: be made into the tuna fish-egg sialoglycopeptide crude product
Concentration is the solution of 100-150mg/ml, and proper amount of active carbon is added and adsorbs sialoglycopeptide.
Further, the dosage of the active carbon is 10mg/ml, adsorption time 60-120min.
Further, methanol-water solution described in step (5) is eluted are as follows: the NaCl- for being 4-6% with mass fraction
The methanol solution sialoglycopeptide in activity eluted charcoal in three times, collects eluent, the bag filter for being 200Da through molecule interception
It after dialysis, is lyophilized after reduced pressure, obtains tuna fish-egg sialoglycopeptide sterling.
Further, the volume fraction of the methanol is 5-8%.
The present invention provides a kind of methods that sialoglycopeptide is prepared from tuna ovum, compensate for the processing of China's tuna
Blank of the industry in terms of sialoglycopeptide.This method is to obtain tuna through ultrasonic wave auxiliary water extraction method using degreasing fish-egg
Ovum sialoglycopeptide crude product is eluted after -50 post separation of sephadex G and activated carbon adsorption using methanol-water solution
Tuna ovum sialoglycopeptide sterling is obtained afterwards.The preparation method was both simple and convenient, and recovery rate is higher, and improved the peace of product
Quan Xing.
Specific embodiment
The present invention provides a kind of method that sialoglycopeptide is prepared from tuna ovum, comprising the following steps:
Tuna fish-egg removes egg membrane, is freeze-dried after homogenate；
The fish-egg of freeze-drying dries after dehydrated alcohol degreasing；
Lower extraction sialoglycopeptide is assisted in ultrasonic wave through deionized water；
It is concentrated under reduced pressure after -50 post separation of sephadex G；
Activated carbon adsorption is eluted through methanol-water solution, obtains tuna ovum sialoglycopeptide sterling.
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, With reference to embodiment
The present invention is described in further detail.
A method of sialoglycopeptide is prepared from tuna ovum, comprising:
Step 1: tuna fish-egg removes egg membrane, is freeze-dried after homogenate；
The step specific as follows can execute: solve under conditions of temperature is 4 DEG C after the tuna fish-egg of freezing is taken out
Freeze, be freeze-dried 12h under conditions of temperature is -20 DEG C after homogenate, obtains tuna fish-egg powder.
Step 2: the fish-egg of freeze-drying dries after dehydrated alcohol degreasing；
The step specific as follows can execute: the tuna fish-egg powder be added in the dehydrated alcohol of 10-15 times of volume
It stirs 10h and carries out degreasing, during which every 3h replaces a dehydrated alcohol, filters rouge by bilayer after degreasing and is filtered to remove ethyl alcohol, takes filter
Slag air-dries 6-10h in cold wind drier and obtains degreasing tuna fish-egg powder.
Step 3: lower extraction sialoglycopeptide is assisted in ultrasonic wave through deionized water；
The step specific as follows can execute: by the degreasing tuna fish-egg powder by 20-30 times of its w/v
Sialoglycopeptide is carried out in addition deionized water extracts 12-16h under conditions of temperature is 4-10 DEG C, during milking per small
Shi Jinhang ultrasonication 10-30min carries out centrifugation 10-15min after acquisition, takes to assist obtaining sialoglycopeptide
Supernatant is concentrated under reduced pressure at 40-45 DEG C, obtains concentrate, wherein ultrasonic sound field intensity is 200~300W/cm2, surpass
Acoustic frequency is 30kHz, centrifugal rotational speed 8000r/min.
Step 4: it is concentrated under reduced pressure after -50 post separation of sephadex G；
The step specific as follows can execute: the concentrate be separated through -50 column of sephadex G, temperature is
20-30 DEG C, flow velocity 1.0-1.5ml/min, every 7min collection eluent judges the saliva in eluent with high performance liquid chromatography
It is de- whether liquid acid glycopeptide flows, and collects the eluent containing sialoglycopeptide and is concentrated under reduced pressure at 40-45 DEG C, is freeze-dried,
Obtain tuna fish-egg sialoglycopeptide crude product.
Step 5: activated carbon adsorption is eluted through methanol-water solution, obtains tuna ovum sialoglycopeptide sterling.
The step specific as follows can execute: it is 100- that the tuna fish-egg sialoglycopeptide crude product, which is made into concentration,
The solution of 150mg/ml is added proper amount of active carbon and adsorbs sialoglycopeptide, and the dosage of the active carbon is 10mg/ml, when absorption
Between be 60-120min, the NaCl- methanol solution for being 4-6% with the mass fraction sialoglycopeptide in activity eluted charcoal in three times,
Eluent is collected, after the bag filter dialysis that molecule interception is 200Da, is lyophilized after reduced pressure, obtains tuna fish-egg saliva
Sour glycopeptide sterling.
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, further below with reference to embodiment
Illustrate technical solution of the present invention.But the present invention is not limited to listed embodiments, should also be included in of the presently claimed invention
Other any well known changes in interest field.
" one embodiment " or " embodiment " referred to herein, which refers to, may be included at least one implementation of the invention
A particular feature, structure, or characteristic." in one embodiment " that different places occur in the present specification not refers both to same
A embodiment, nor the individual or selective embodiment mutually exclusive with other embodiments.
Embodiment 1
The implementation case shows a kind of method that sialoglycopeptide is prepared from tuna ovum as follows:
The tuna fish-egg of freezing thaws at 4 DEG C after taking out, and after being homogenized using refiner, freezes 12h at -20 DEG C,
It is lyophilized using freeze drier, obtains tuna fish-egg powder.The fish-egg powder of freeze-drying be added 10 times of volumes dehydrated alcohol (1/10,
W/v) stirring 10h carries out degreasing, and during which every 3h replaces a dehydrated alcohol.The double-deck filter rouge is filtered to remove ethyl alcohol, filter residue after degreasing
6h is air-dried in cold wind drier obtains degreasing tuna fish-egg powder.Extracting degreasing fish-egg powder is added by 20 times of its w/v
Deionized water carries out the extraction (1/20, w/v) of sialoglycopeptide, and Extracting temperature is 4 DEG C, extraction time 12h.Period is per hour
Ultrasonication is carried out with assisted extraction sialoglycopeptide, the processing time is 10min, and ultrasonic sound field intensity is 200W/cm2, surpass
Acoustic frequency is 30kHz.It is centrifuged after extraction, 8000r/min, is centrifuged 10min.Supernatant is taken to be depressurized at 40 DEG C
Concentration.- 50 column of sephadex G is separated on concentrate, and temperature is 20 DEG C, and flow velocity 1.0ml/min, every 7min collection is washed
De- liquid, it is de- to judge whether the sialoglycopeptide in eluent flows with high performance liquid chromatography.Collect washing containing sialoglycopeptide
De- liquid is concentrated under reduced pressure at 40 DEG C, is freeze-dried to obtain tuna fish-egg sialoglycopeptide crude product.By the tuna fish of freeze-drying
Ovum sialoglycopeptide crude product is made into the solution that concentration is 100mg/ml, and proper amount of active carbon is added and adsorbs sialoglycopeptide, active carbon
Dosage is 10mg/ml, adsorption time 60min, and (volume fraction of methanol is the NaCl- methanol solution for being 4% with mass fraction
5%) the sialoglycopeptide in charcoal activity eluted in three times collects eluent, saturating for the bag filter of 200Da through molecule interception
It after analysis, is lyophilized after reduced pressure, obtains tuna fish-egg sialoglycopeptide sterling.
Embodiment 2
The implementation case shows a kind of method that sialoglycopeptide is prepared from tuna ovum: the gold of freezing as follows
Marlin fish-egg thaws at 4 DEG C after taking out, and after being homogenized using refiner, freezes 12h at -20 DEG C, is frozen using freeze drier
It is dry, obtain tuna fish-egg powder.Dehydrated alcohol (1/12, w/v) the stirring 10h that 13 times of volumes are added in the fish-egg powder of freeze-drying is taken off
Rouge, during which every 3h replaces a dehydrated alcohol.The double-deck filter rouge is filtered to remove ethyl alcohol after degreasing, and filter residue air-dries in cold wind drier
8h obtains degreasing tuna fish-egg powder.Extracting degreasing fish-egg powder carries out sialic acid by 25 times of addition deionized waters of its w/v
The extraction (1/25, w/v) of glycopeptide, Extracting temperature are 8 DEG C, extraction time 14h.Period carries out ultrasonication per hour with auxiliary
Extraction sialoglycopeptide is helped, the processing time is 20min, and ultrasonic sound field intensity is 250W/cm2, supersonic frequency 30kHz.It extracts
After be centrifuged, 8000r/min, be centrifuged 13min.Supernatant is taken to be concentrated under reduced pressure at 43 DEG C.Portugal is poly- on concentrate
Sugared gel G-50 column is separated, and temperature is 25 DEG C, and flow velocity 1.2ml/min, every 7min collection eluent uses high-efficient liquid phase color
It is de- that spectrometry judges whether the sialoglycopeptide in eluent flows.The eluent containing sialoglycopeptide is collected to be subtracted at 43 DEG C
Pressure concentration, is freeze-dried to obtain tuna fish-egg sialoglycopeptide crude product.The tuna fish-egg sialoglycopeptide crude product of freeze-drying is matched
The solution for being 125mg/ml at concentration is added proper amount of active carbon and adsorbs sialoglycopeptide, activated carbon dosage 10mg/ml, absorption
Time is 95min, and the elution in three times of the NaCl- methanol solution for being 5% with mass fraction (volume fraction of methanol is 7%) is living
Property charcoal in sialoglycopeptide, collect eluent, through molecule interception be 200Da bag filter dialysis after, freeze after reduced pressure
It is dry, obtain tuna fish-egg sialoglycopeptide sterling.
Embodiment 3
The implementation case shows a kind of method that sialoglycopeptide is prepared from tuna ovum as follows:
The tuna fish-egg of freezing thaws at 4 DEG C after taking out, and after being homogenized using refiner, freezes 12h at -20 DEG C,
It is lyophilized using freeze drier, obtains tuna fish-egg powder.The fish-egg powder of freeze-drying be added 15 times of volumes dehydrated alcohol (1/15,
W/v) stirring 10h carries out degreasing, and during which every 3h replaces a dehydrated alcohol.The double-deck filter rouge is filtered to remove ethyl alcohol, filter residue after degreasing
10h is air-dried in cold wind drier obtains degreasing tuna fish-egg powder.Extracting degreasing fish-egg powder by its w/v 30 extraordinarily
Enter the extraction (1/30, w/v) that deionized water carries out sialoglycopeptide, Extracting temperature is 10 DEG C, extraction time 16h.Period is every
Hour carries out ultrasonication with assisted extraction sialoglycopeptide, and the processing time is 30min, and ultrasonic sound field intensity is 300W/
cm2, supersonic frequency 30kHz.It is centrifuged after extraction, 8000r/min, is centrifuged 15min.Take supernatant at 45 DEG C into
Row is concentrated under reduced pressure.- 50 column of sephadex G is separated on concentrate, and temperature is 30 DEG C, flow velocity 1.5ml/min, every 7min
Eluent is collected, it is de- to judge whether the sialoglycopeptide in eluent flows with high performance liquid chromatography.It collects containing sialic acid sugar
The eluent of peptide is concentrated under reduced pressure at 45 DEG C, is freeze-dried to obtain tuna fish-egg sialoglycopeptide crude product.By the gold of freeze-drying
Marlin fish-egg sialoglycopeptide crude product is made into the solution that concentration is 150mg/ml, and proper amount of active carbon is added and adsorbs sialoglycopeptide,
Activated carbon dosage is 10mg/ml, adsorption time 120min, the NaCl- methanol solution (body of methanol for being 6% with mass fraction
Fraction is the sialoglycopeptide in charcoal activity eluted in three times 8%), collects eluent, is 200Da's through molecule interception
After bag filter dialysis, it is lyophilized after reduced pressure, obtains tuna fish-egg sialoglycopeptide sterling.
Following table 1 is the comparison of three embodiments and present technology, specific as follows:
Table 1
Compared with prior art, the beneficial effects of the present invention are: production process is simple, the period is short, simple and convenient, recovery rate
It is higher, with high purity, Product Safety is high.
It should be noted that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferable
Embodiment describes the invention in detail, those skilled in the art should understand that, it can be to technology of the invention
Scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered in this hair
In bright scope of the claims.
Claims (10)
1. a kind of method for preparing sialoglycopeptide from tuna ovum, which is characterized in that this method comprises the following steps:
(1) it thaws after taking out the tuna fish-egg of freezing, be freeze-dried after homogenate, obtain tuna fish-egg powder；
(2) the tuna fish-egg powder is filtered after dehydrated alcohol degreasing and is dried, obtain degreasing tuna fish-egg powder；
(3) the degreasing tuna fish-egg powder is assisted into lower extraction, centrifugation, concentration in ultrasonic wave through deionized water, is concentrated
Liquid；
(4) concentrate is concentrated under reduced pressure after -50 post separation of sephadex G, obtains tuna fish-egg sialoglycopeptide
Crude product；
(5) the tuna fish-egg sialoglycopeptide crude product is utilized into activated carbon adsorption, is eluted, is obtained through methanol-water solution
Tuna fish-egg sialoglycopeptide sterling.
2. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, it is characterised in that: step
(1) temperature of defrosting described in is 4 DEG C, and the temperature of the freezing is -20 DEG C, time 12h.
3. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, which is characterized in that step
(2) the tuna fish-egg powder is filtered after dehydrated alcohol degreasing described in and is dried, obtaining degreasing tuna fish-egg powder includes:
Stirring 10h in the dehydrated alcohol of 10-15 times of volume is added in the tuna fish-egg powder and carries out degreasing, during which every 3h replacement is primary
Dehydrated alcohol filters rouge by bilayer after degreasing and is filtered to remove ethyl alcohol, takes filter residue to air-dry 6-10h in cold wind drier and obtains degreasing
Tuna fish-egg powder.
4. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, which is characterized in that step
(3) the degreasing tuna fish-egg powder is assisted into lower extraction, centrifugation, concentration in ultrasonic wave through deionized water described in, is obtained dense
Contracting liquid includes: that the degreasing tuna fish-egg powder is added in deionized water to carry out saliva by 20-30 times of its w/v
The extraction of sour glycopeptide carries out ultrasonication to assist obtaining sialoglycopeptide, after acquisition per hour during milking
It is centrifuged, supernatant is taken to be concentrated under reduced pressure at 40-45 DEG C, obtain concentrate.
5. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 4, it is characterised in that: described
Extracting temperature is 4-10 DEG C, extraction time 12-16h, and the ultrasonic treatment time is 10-30min, and ultrasonic sound field intensity is
200~300W/cm2, supersonic frequency 30kHz, centrifugal rotational speed 8000r/min, centrifugation time 10-15min.
6. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, which is characterized in that step
(4) concentrate is concentrated under reduced pressure after -50 post separation of sephadex G described in, obtains tuna fish-egg sialic acid sugar
Peptide crude product includes: to separate the concentrate through -50 column of sephadex G, and temperature is 20-30 DEG C, flow velocity 1.0-
1.5ml/min, every 7min collect eluent, it is de- to judge whether the sialoglycopeptide in eluent flows with high performance liquid chromatography,
It collects the eluent containing sialoglycopeptide to be concentrated under reduced pressure at 40-45 DEG C, is freeze-dried, obtains tuna fish-egg saliva
Sour glycopeptide crude product.
7. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, which is characterized in that step
(5) activated carbon adsorption described in are as follows: it is 100-150mg/ml's that the tuna fish-egg sialoglycopeptide crude product, which is made into concentration,
Solution is added proper amount of active carbon and adsorbs sialoglycopeptide.
8. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 7, it is characterised in that: described
The dosage of active carbon is 10mg/ml, adsorption time 60-120min.
9. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 1, which is characterized in that step
(5) methanol-water solution described in is eluted are as follows: the NaCl- methanol solution for being 4-6% with mass fraction is activity eluted in three times
Sialoglycopeptide in charcoal collects eluent, after the bag filter dialysis that molecule interception is 200Da, is lyophilized after reduced pressure,
Obtain tuna fish-egg sialoglycopeptide sterling.
10. a kind of method for preparing sialoglycopeptide from tuna ovum according to claim 9, it is characterised in that: institute
The volume fraction for stating methanol is 5-8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811260150.0A CN109280081B (en) | 2018-10-26 | 2018-10-26 | Method for preparing sialoglycopeptide from tuna eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811260150.0A CN109280081B (en) | 2018-10-26 | 2018-10-26 | Method for preparing sialoglycopeptide from tuna eggs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109280081A true CN109280081A (en) | 2019-01-29 |
| CN109280081B CN109280081B (en) | 2021-06-25 |
Family
ID=65177949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811260150.0A Active CN109280081B (en) | 2018-10-26 | 2018-10-26 | Method for preparing sialoglycopeptide from tuna eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109280081B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050866A (en) * | 2009-11-04 | 2011-05-11 | 上海来益生物药物研究开发中心有限责任公司 | Method for purifying glycopeptide compound |
| CN102382178A (en) * | 2011-10-21 | 2012-03-21 | 山东大学 | Separation method of sialoglycopeptide |
| CN103114117A (en) * | 2013-01-24 | 2013-05-22 | 中国海洋大学 | Method for preparing fish egg sugar protein with anti-osteoporosis effect |
| CN107868808A (en) * | 2017-11-01 | 2018-04-03 | 金腰燕（广州）食品有限公司 | A kind of method for improving bird's nest saliva free acid |
-
2018
- 2018-10-26 CN CN201811260150.0A patent/CN109280081B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050866A (en) * | 2009-11-04 | 2011-05-11 | 上海来益生物药物研究开发中心有限责任公司 | Method for purifying glycopeptide compound |
| CN102382178A (en) * | 2011-10-21 | 2012-03-21 | 山东大学 | Separation method of sialoglycopeptide |
| CN103114117A (en) * | 2013-01-24 | 2013-05-22 | 中国海洋大学 | Method for preparing fish egg sugar protein with anti-osteoporosis effect |
| CN107868808A (en) * | 2017-11-01 | 2018-04-03 | 金腰燕（广州）食品有限公司 | A kind of method for improving bird's nest saliva free acid |
Non-Patent Citations (6)
| Title |
|---|
| 丛峰松等: "《神奇的小分子活性肽 修订版》", 30 September 2018, 上海交通大学出版社 * |
| 任国艳等: "《海蜇糖蛋白的特性及免疫活性》", 30 September 2017, 中国原子能出版社 * |
| 周晓春: ""鲫鱼卵唾液酸糖蛋白对老年性骨质疏松症小鼠的改善作用研究"", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
| 王姗姗等: ""鲫鱼卵唾液酸糖蛋白对去卵巢大鼠骨质疏松症的改善作用"", 《食品科学》 * |
| 胡建平等: "《鱼胶原蛋白的开发与应用》", 31 March 2014, 四川大学出版社 * |
| 钟天白等: ""唾液酸糖肽的研究进展"", 《生物技术通讯》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109280081B (en) | 2021-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2020048049A1 (en) | Method for preparing luo han guo sweetener composition from siraitia grosvenorii and use thereof | |
| CN106220693B (en) | A method of extracting multiple-ear rock Ke phloridzin | |
| CN101812137B (en) | Method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin | |
| CN104152519B (en) | A kind of preparation method of enzyme dissolubility high-purity superhelix NTx albumen | |
| CN103735633B (en) | A kind of preparation method of instant gardenia powder | |
| CN102389102A (en) | Low-sodium compound salt | |
| CN102746412A (en) | Method for extracting momordica charantiap polysaccharide | |
| CN101974095B (en) | Method for extracting and separating Chinese narcissus polysaccharides | |
| CN109180755A (en) | A method of extracting Rubusoside from sweet tea | |
| CN105924495B (en) | High-efficiency preparation method of high-purity flaxseed protein | |
| CN107602665A (en) | A kind of brown croaker flesh of fish anti-oxidation peptide | |
| CN109943612A (en) | Method for producing high-activity minced fillet and product antifreeze agent by enzymolysis of silver carp scales | |
| CN101664543A (en) | Hemokinin-iron oral liquid | |
| CN104744601B (en) | Method for extracting and purifying fleurotus ferulae polysaccharide | |
| CN107319400A (en) | Bioactive ingredients method for integrated extraction in oyster | |
| CN112707852B (en) | Combined preparation method of garlic extract | |
| CN104844721B (en) | Extraction and separation method of Agrocybe aegirit polysaccharides | |
| CN109280081A (en) | A method of sialoglycopeptide is prepared from tuna ovum | |
| WO2021082311A1 (en) | Pea peptide having supplementary blood glucose reducing function and preparation method therefor | |
| CN102224921B (en) | Polypeptide salt substitute and preparation method thereof | |
| CN110483655A (en) | A kind of Polysaccharide from Portulaca oleracea extraction process and its application in skin care item | |
| CN104131060B (en) | Corbicula fluminea anti-oxidative peptide and preparation method thereof | |
| JP4406032B2 (en) | Scallop polysaccharide extraction method | |
| CN106107242A (en) | A kind of nonreactive aquatic immune reinforcing agent | |
| CN109810177A (en) | A kind of walnut dregs polypeptide and its preparation method and application with ACE inhibitory activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |