CN109277582A - A kind of production method of biological nano silver - Google Patents
A kind of production method of biological nano silver Download PDFInfo
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- CN109277582A CN109277582A CN201811213799.7A CN201811213799A CN109277582A CN 109277582 A CN109277582 A CN 109277582A CN 201811213799 A CN201811213799 A CN 201811213799A CN 109277582 A CN109277582 A CN 109277582A
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- nano silver
- biological nano
- production method
- culture medium
- silver
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
Abstract
The invention discloses a kind of production methods of biological nano silver, including the following steps: 1) takes Exserohilum turcicum to be added in culture medium and cultivated；2) it takes cultured Exserohilum turcicum 2~4 times wash with distilled water, removes the interfering substance in culture medium；3) 60~80h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water, the liquid after immersion is put into centrifuge and is centrifuged 10~20min at 3500~5000r/min, isolates soak；4) soak isolated in step 3) is taken, silver nitrate solution is added into soak, final concentration of 1.5~the 2.5mmol/L of silver ion in mixed solution, it is stored at room temperature 20~30h, biological nano silver is made, technical process is simple and easy, and reaction condition is mild, easily controllable, has a good application prospect.
Description
Technical field
The present invention relates to field of nanometer material technology, more specifically, being a kind of production method of biological nano silver.
Background technique
Silver nano material has highly stable physical and chemical performance, has obtained extensively in the fields such as ceramics and environment-friendly materials
General application.Currently, silver nano material is still the hot spot of research, application prospect is more wide.
Currently, the preparation method of Nano silver grain is varied, according to the difference of preparating mechanism, can be divided into following several:
Chemical reduction method, electrochemical process, photochemical reduction, radiation method, laser ablation method, colloidal sol-gel method, vacuum vapour deposition, change
Learn galvanoplastic, crystal seed method etc..And the silver nano material prepared can be applied to the fields such as optics, catalyst, anti-biotic material.
Antibacterial material includes natural, organic and inorganic antibacterial material three categories type, compared with natural and organic anti-bacterial material,
Inorganic antibacterial material has apparent toxicity low, heat-resist, and the duration is long, has a broad antifungal spectrum, is a kind of with good business
The antibacterial agent kind of prospect.
Silver ion is as a kind of strongest metal ion of biocidal property, using earliest and most extensive.Micro Ag+ is to human body
Be it is beneficial, but have destruction to microorganism.Silver is a kind of time-honored bacteriostatic agent, and people are begun in ancient times using silver
Utensil saves food, and to early 20th century, silver nitrate solution is just by one of surgery founder Halstead for handling wound and scalding
Wound.
The preparation of inorganic antibacterial material has physics, chemistry, biological method at present, since chemically and physically method preparation is related to
To toxic or radioreagent, and biological method becomes silver nano technology development in recent years because it has reliable, Green Features
Trend.
Summary of the invention
In order to make up the above deficiency, the present invention provides a kind of production methods of biological nano silver.
The scheme of the invention is:
A kind of production method of biological nano silver, including the following steps:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 2~4 times wash with distilled water, removes the interfering substance in culture medium；
3) 60~80h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water,
Liquid after immersion is put into centrifuge and is centrifuged 10~20min at 3500~5000r/min, isolates soak；
4) take the soak isolated in step 3), silver nitrate solution be added into soak, in mixed solution silver from
Final concentration of 1.5~the 2.5mmol/L of son, is stored at room temperature 20~30h, reacts synthesising biological nano silver, reaction process by the two
Middle mixed solution fades to dark-brown by colourless, and biological nano silver is made.
The culture medium is PDA culture medium as a preferred technical solution,.
It is taken in the step 2) as a preferred technical solution, cultured Exserohilum turcicum 3 times wash with distilled water.
72h is impregnated in the step 3) as a preferred technical solution,.
Liquid after impregnating in the step 3) as a preferred technical solution, be put into centrifuge at 4000r/min from
Heart 15min.
The final concentration of 2mmol/L of silver ion in the solution mixed in the step 4) as a preferred technical solution,.
It is stored at room temperature for 24 hours in the step 4) as a preferred technical solution,.
The Exserohilum turcicum is the big spot Exserohilum of Deuteromycotina fungi as a preferred technical solution,.
The present invention also provides a kind of biological nano silver, and the biological nano silver is by any biological nano of claim 1-8
The production method of silver is made.
By adopting the above-described technical solution, a kind of production method of biological nano silver, including the following steps: 1) take jade
Rice Exserohilum Turcicum, which is added in culture medium, to be cultivated；2) it takes cultured Exserohilum turcicum 2~4 times wash with distilled water,
Remove the interfering substance in culture medium；3) then toward go in the cleaned culture medium containing Exserohilum turcicum be added distillation
Water, impregnates 60~80h, and the liquid after immersion is put into centrifuge and is centrifuged 10~20min at 3500~5000r/min, isolates
Soak；4) soak isolated in step 3) is taken, silver nitrate solution, silver ion in mixed solution are added into soak
Final concentration of 1.5~2.5mmol/L, is stored at room temperature 20~30h, synthesising biological nano silver is reacted by the two, in reaction process
Mixed solution fades to dark-brown by colourless, and biological nano silver is made.
Invention advantage: technical process is simple and easy, and reaction condition is mild, easily controllable, and using biological method preparation silver
Nano material avoids toxic or radioreagent chemical, in physical method, has the advantages that reliable, green；Dispersibility
Good, narrow particle size distribution has a good application prospect.
Specific embodiment
In order to make up the above deficiency, the present invention provides a kind of production methods of biological nano silver, to solve above-mentioned background
The problems in technology.
A kind of production method of biological nano silver, including the following steps:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 2~4 times wash with distilled water, removes the interfering substance in culture medium；
3) 60~80h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water,
Liquid after immersion is put into centrifuge and is centrifuged 10~20min at 3500~5000r/min, isolates soak；
4) take the soak isolated in step 3), silver nitrate solution be added into soak, in mixed solution silver from
Final concentration of 1.5~the 2.5mmol/L of son, is stored at room temperature 20~30h, reacts synthesising biological nano silver, reaction process by the two
Middle mixed solution fades to dark-brown by colourless, and biological nano silver is made.
The culture medium is PDA culture medium.
It is taken in the step 2) cultured Exserohilum turcicum 3 times wash with distilled water.
72h is impregnated in the step 3).
Liquid after impregnating in the step 3) is put into centrifuge and is centrifuged 15min at 4000r/min.
The final concentration of 2mmol/L of silver ion in the solution mixed in the step 4).
It is stored at room temperature for 24 hours in the step 4).
The Exserohilum turcicum is the big spot Exserohilum of Deuteromycotina fungi.
The present invention also provides a kind of biological nano silver, and the biological nano silver is by any biological nano of claim 1-8
The production method of silver is made.
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment one:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 2 times wash with distilled water, removes the interfering substance in culture medium；
3) 60h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water, impregnated
Liquid afterwards is put into centrifuge and is centrifuged 10min at 3500r/min, isolates soak；
4) take the soak isolated in step 3), silver nitrate solution be added into soak, in mixed solution silver from
The final concentration of 1.5mmol/L of son, is stored at room temperature 20h, reacts synthesising biological nano silver, mixed solution in reaction process by the two
Dark-brown is faded to by colourless, biological nano silver is made.
The culture medium is PDA culture medium.
The Exserohilum turcicum is the big spot Exserohilum of Deuteromycotina fungi.
Embodiment two:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 4 times wash with distilled water, removes the interfering substance in culture medium；
3) 80h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water, impregnated
Liquid afterwards is put into centrifuge and is centrifuged 20min at 5000r/min, isolates soak；
4) take the soak isolated in step 3), silver nitrate solution be added into soak, in mixed solution silver from
The final concentration of 2.5mmol/L of son, is stored at room temperature 30h, reacts synthesising biological nano silver, mixed solution in reaction process by the two
Dark-brown is faded to by colourless, biological nano silver is made.
The culture medium is PDA culture medium.
The Exserohilum turcicum is the big spot Exserohilum of Deuteromycotina fungi.
Embodiment three:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 3 times wash with distilled water, removes the interfering substance in culture medium；
3) 72h then is impregnated toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water, impregnated
Liquid afterwards is put into centrifuge and is centrifuged 15min at 4000r/min, isolates soak；
4) take the soak isolated in step 3), silver nitrate solution be added into soak, in mixed solution silver from
The final concentration of 2mmol/L of son, is stored at room temperature for 24 hours, reacts synthesising biological nano silver by the two, in reaction process mixed solution by
It is colourless to fade to dark-brown, biological nano silver is made.
The culture medium is PDA culture medium.
The Exserohilum turcicum is the big spot Exserohilum of Deuteromycotina fungi.
The above shows and describes the basic principle, main features and advantages of the invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent circle.
Claims (9)
1. a kind of production method of biological nano silver, characterized in that it comprises the following steps:
1) it takes Exserohilum turcicum to be added in culture medium to be cultivated；
2) it takes cultured Exserohilum turcicum 2~4 times wash with distilled water, removes the interfering substance in culture medium；
3) then 60~80h is impregnated, after immersion toward removing in the cleaned culture medium containing Exserohilum turcicum to be added distilled water
Liquid be put into centrifuge and be centrifuged 10~20min at 3500~5000r/min, isolate soak；
4) soak isolated in step 3) is taken, silver nitrate solution is added into soak, silver ion is whole in mixed solution
Concentration is 1.5~2.5mmol/L, is stored at room temperature 20~30h, reacts synthesising biological nano silver by the two, mixes in reaction process
It closes solution and fades to dark-brown by colourless, biological nano silver is made.
2. a kind of biological nano silver production method as described in claim 1, it is characterised in that: the culture medium is PDA culture
Base.
3. a kind of biological nano silver production method as described in claim 1, it is characterised in that: take and cultivate in the step 2)
Exserohilum turcicum 3 times wash with distilled water.
4. a kind of biological nano silver production method as described in claim 1, it is characterised in that: impregnate 72h in the step 3).
5. a kind of biological nano silver production method as described in claim 1, it is characterised in that: after being impregnated in the step 3)
Liquid is put into centrifuge and is centrifuged 15min at 4000r/min.
6. a kind of biological nano silver production method as described in claim 1, it is characterised in that: what is mixed in the step 4) is molten
The final concentration of 2mmol/L of silver ion in liquid.
7. a kind of biological nano silver production method as described in claim 1, it is characterised in that: be stored at room temperature in the step 4)
24h。
8. a kind of biological nano silver production method as described in claim 1, it is characterised in that: the Exserohilum turcicum is half
Know the big spot Exserohilum of bacterium subphylum fungi.
9. a kind of biological nano silver, it is characterised in that: the biological nano silver is by any biological nano silver of claim 1-8
Production method is made.
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| CN201811213799.7A CN109277582A (en) | 2018-10-18 | 2018-10-18 | A kind of production method of biological nano silver |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602109A (en) * | 2009-06-15 | 2009-12-16 | 中北大学 | A kind of preparation method of silver nano material |
| US20110274736A1 (en) * | 2010-05-10 | 2011-11-10 | King Saud University | Methods for producing silver nanoparticles |
| CN102638974A (en) * | 2009-12-02 | 2012-08-15 | 巴斯夫欧洲公司 | Method for treating phytopathogenic microorganisms using surface-modified nanoparticulate copper salts |
| CN102962467A (en) * | 2012-10-26 | 2013-03-13 | 上海交通大学 | Method for preparing noble metal nano material with adjustable particle size by bacteria |
| CN103331454A (en) * | 2013-07-02 | 2013-10-02 | 南京大学 | Nano-silver manufacturing technology |
| JP2015010063A (en) * | 2013-06-28 | 2015-01-19 | 八千代工業株式会社 | Antimicrobial material |
| CN105935781A (en) * | 2016-06-06 | 2016-09-14 | 江苏省农业科学院 | Biological method for preparing nano-silver |
| US9491947B1 (en) * | 2015-09-28 | 2016-11-15 | King Saud University | Method of synthesizing nanoparticles and a nanoparticle-polymer composite using a plant extract |
| CN106112008A (en) * | 2016-08-18 | 2016-11-16 | 安徽科技学院 | Utilize the method that Flos Osmanthi Fragrantis blade prepares nanometer silver |
| CN106623972A (en) * | 2016-12-16 | 2017-05-10 | 上海理工大学 | Method for preparing silver nanoparticles from Chinese wolfberry fruit extraction liquid |
| CN107052360A (en) * | 2017-04-13 | 2017-08-18 | 湖南工业大学 | A kind of method that utilization spine spore mould prepares Nano Silver |
-
2018
- 2018-10-18 CN CN201811213799.7A patent/CN109277582A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602109A (en) * | 2009-06-15 | 2009-12-16 | 中北大学 | A kind of preparation method of silver nano material |
| CN102638974A (en) * | 2009-12-02 | 2012-08-15 | 巴斯夫欧洲公司 | Method for treating phytopathogenic microorganisms using surface-modified nanoparticulate copper salts |
| US20110274736A1 (en) * | 2010-05-10 | 2011-11-10 | King Saud University | Methods for producing silver nanoparticles |
| CN102962467A (en) * | 2012-10-26 | 2013-03-13 | 上海交通大学 | Method for preparing noble metal nano material with adjustable particle size by bacteria |
| JP2015010063A (en) * | 2013-06-28 | 2015-01-19 | 八千代工業株式会社 | Antimicrobial material |
| CN103331454A (en) * | 2013-07-02 | 2013-10-02 | 南京大学 | Nano-silver manufacturing technology |
| US9491947B1 (en) * | 2015-09-28 | 2016-11-15 | King Saud University | Method of synthesizing nanoparticles and a nanoparticle-polymer composite using a plant extract |
| CN105935781A (en) * | 2016-06-06 | 2016-09-14 | 江苏省农业科学院 | Biological method for preparing nano-silver |
| CN106112008A (en) * | 2016-08-18 | 2016-11-16 | 安徽科技学院 | Utilize the method that Flos Osmanthi Fragrantis blade prepares nanometer silver |
| CN106623972A (en) * | 2016-12-16 | 2017-05-10 | 上海理工大学 | Method for preparing silver nanoparticles from Chinese wolfberry fruit extraction liquid |
| CN107052360A (en) * | 2017-04-13 | 2017-08-18 | 湖南工业大学 | A kind of method that utilization spine spore mould prepares Nano Silver |
Non-Patent Citations (1)
| Title |
|---|
| 王晓鸣，王会伟: "《玉米大斑病小斑病及其防治》", 31 July 2009, 北京：金盾出版社 * |
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Application publication date: 20190129 |