CN109276472A - A kind of cooperation micropin uses the preparation method and application of autologous collagen albumen stoste - Google Patents
A kind of cooperation micropin uses the preparation method and application of autologous collagen albumen stoste Download PDFInfo
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- CN109276472A CN109276472A CN201811283686.4A CN201811283686A CN109276472A CN 109276472 A CN109276472 A CN 109276472A CN 201811283686 A CN201811283686 A CN 201811283686A CN 109276472 A CN109276472 A CN 109276472A
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- CN
- China
- Prior art keywords
- collagen albumen
- autologous collagen
- micropin
- albumen stoste
- cooperation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0204—Specific forms not provided for by any of groups A61K8/0208 - A61K8/14
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/91—Injection
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Hematology (AREA)
- Medical Informatics (AREA)
- Heart & Thoracic Surgery (AREA)
- Biomedical Technology (AREA)
- Anesthesiology (AREA)
- Emergency Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
Abstract
The embodiment of the invention discloses preparation methods and application that a kind of cooperation micropin uses autologous collagen albumen stoste, the preparation method for the autologous collagen albumen stoste that the cooperation micropin uses, including produce self fibr tissue suspension, carry out digestion reaction, saltout and finally synthesize.The preparation method for the autologous collagen albumen stoste that cooperation micropin provided in an embodiment of the present invention uses is by using autologous collagen albumen stoste, it can effectively solve the problem that micropin easily leads to the problem of rejection to skin, damages to skin, ensure that the compatibility of body inner tissue.It in addition, the application also proposed a kind of micropin, can effectively cooperate the use of above-mentioned autologous collagen albumen stoste, provide very big convenience to people.
Description
Technical field
The present embodiments relate to medical cosmetology technical fields, and in particular to a kind of cooperation micropin uses autologous collagen albumen
The preparation method and application of stoste.
Background technique
Micropin beauty is a kind of beauty method of very fashion, stimulates skin using small syringe needles many on micropin idler wheel,
Micropin can make more than 200,000 microtubules and reach beauty by the regeneration of stimulation collagen within a very short time
Purpose.But existing micropin is when in use, easily generates rejection to skin, damages to skin, be unfavorable for people
It uses.
Summary of the invention
The embodiment of the present invention is designed to provide a kind of preparation method for cooperating micropin to use autologous collagen albumen stoste
Rejection easily is generated to skin, the problem of damaging to skin to solve existing micropin when in use with application.
To achieve the above object, the technical solution of the embodiment of the present invention are as follows: a kind of cooperation micropin uses autologous collagen albumen
The preparation method of stoste, comprising the following steps:
It impregnates, is during which kept stirring until at paste Step 1: mature self fibr tissue is added in ultrapure water；
The self fibr tissue of paste is crushed and is put into ultrapure water, self fibr tissue suspension is obtained, to self fibr tissue
The obtained mixture of acetic acid on the rocks, spare in suspension；
Step 2: pepsin solution is added in the mixture prepared to step 1 carries out digestion reaction, digestion reaction knot
Upper layer suspension is put into a centrifuge centrifugation by stratification after beam, collects supernatant, spare；
Step 3: the supernatant that step 2 is collected is mixed with NaCl solution, it is centrifuged later, collects supernatant and make
Obtain crude autologous collagen albumen stoste；
Step 4: crude autologous collagen albumen stoste is mixed with hyaluronic acid solution, citric acid solution, it is centrifuged later simultaneously
Filtering obtains the autologous collagen albumen stoste.
Among some embodiments, the self fibr tissue of paste crushes and is put into the ultrapure of 10 times of volumes in step 1
In water.
Among some embodiments, into self fibr tissue suspension, the concentration of mixture made from acetic acid on the rocks is
0.4mg/mL。
Among some embodiments, the preparation method of pepsin solution includes first weighing pepsin in step 2,
Pepsin is dissolved in the pepsin solution that 10mg/mL is made in 0.5M acetic acid later.
Among some embodiments, the step two includes that upper layer suspension is put into revolving speed for 3500-5000r/
It is centrifuged in the centrifuge of min, collects supernatant, repeated above-mentioned centrifugal process 3-5 times, collect supernatant, it is spare.
Among some embodiments, the step three includes the supernatant for collecting step 2 and NaCl solution by weight
Amount proportion mixing, is centrifuged 5-30min in the centrifuge that revolving speed is 3000-1000r/min, discards supernatant liquid, and precipitating uses acetic acid
Dissolution, later repeated centrifugation 3-5 times collect supernatant and obtain crude autologous collagen albumen stoste.
Among some more specific embodiments, it is molten to precipitate the acetic acid for being 0.5mol/L with the concentration of 10 times of volumes
Solution.
Among some embodiments, the mass percent concentration of hyaluronic acid solution described in step 4 is 10-20%,
The mass percent concentration of the citric acid solution is 1-3%.
Among some embodiments, it is centrifuged and filtered autologous collagen albumen stoste can be 0.1-0.5mm from diameter
Micropin in flow out.
In addition, the application also proposed a kind of the micro- of the autologous collagen albumen stoste used applied to above-mentioned cooperation micropin
Needle.
Compared with prior art, the embodiment of the present invention has the advantages that the embodiment of the invention provides a kind of cooperation is micro-
Needle uses the preparation method and application of autologous collagen albumen stoste, the system for the autologous collagen albumen stoste that the cooperation micropin uses
Preparation Method can effectively solve the problem that micropin easily generates rejection to skin, makes to skin by using autologous collagen albumen stoste
The problem of at injury, it ensure that the compatibility of body inner tissue.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
"comprising" or " comprising " as mentioned throughout the specification and claims are an open language, therefore are answered
It is construed to " including but not limited to ".Specification subsequent descriptions are to implement better embodiment of the invention, and so description is
For the purpose of the rule of specification, the range that is not intended to limit the invention.Protection scope of the present invention is when the appended power of view
Benefit requires subject to institute's defender.
Embodiment 1
Present embodiment discloses the preparation methods that a kind of cooperation micropin uses autologous collagen albumen stoste, including following step
It is rapid:
It impregnates, is during which kept stirring until at paste Step 1: mature self fibr tissue is added in ultrapure water；
The self fibr tissue of paste is crushed and is put into the ultrapure water of 10 times of volumes, self fibr tissue suspension is obtained, to certainly
The obtained mixture of acetic acid on the rocks in body fibr tissue suspension, and the concentration of mixture is 0.4mg/mL, it is spare；
Step 2: pepsin solution is produced, and the preparation method of pepsin solution includes first weighing pepsin, it
Pepsin is dissolved in the pepsin solution that 10mg/mL is made in 0.5M acetic acid afterwards, is added in the mixture prepared to step 1
Enter pepsin solution and carry out digestion reaction, stratification after digestion reaction, upper layer suspension, which is put into revolving speed, is
It is centrifuged in 3500r/min centrifuge, collects supernatant, repeated above-mentioned centrifugal process 3 times, collect supernatant, it is spare；
Step 3: the supernatant that step 2 is collected is mixed with NaCl solution, in the centrifuge that revolving speed is 3000r/min
It is centrifuged 5min, discards supernatant liquid, the acetic acid that precipitating is 0.5mol/L with the concentration of 10 times of volumes, repeated centrifugation 3 times later,
It collects supernatant and obtains autologous collagen albumen stoste；
Step 4: crude autologous collagen albumen stoste is mixed with hyaluronic acid solution, citric acid solution, it is centrifuged later simultaneously
Filtering obtains the autologous collagen albumen stoste, and is centrifuged and filtered autologous collagen albumen stoste can be from diameter
It is flowed out in the micropin of 0.1mm；Wherein, the mass percent concentration of the hyaluronic acid solution is 10%, the citric acid solution
Mass percent concentration be 1%.
Embodiment 2
Present embodiment discloses the preparation methods that a kind of cooperation micropin uses autologous collagen albumen stoste, including following step
It is rapid:
It impregnates, is during which kept stirring until at paste Step 1: mature self fibr tissue is added in ultrapure water；
The self fibr tissue of paste is crushed and is put into the ultrapure water of 10 times of volumes, self fibr tissue suspension is obtained, to certainly
The obtained mixture of acetic acid on the rocks in body fibr tissue suspension, and the concentration of mixture is 0.4mg/mL, it is spare；
Step 2: pepsin solution is produced, and the preparation method of pepsin solution includes first weighing pepsin, it
Pepsin is dissolved in the pepsin solution that 10mg/mL is made in 0.5M acetic acid afterwards, is added in the mixture prepared to step 1
Enter pepsin solution and carry out digestion reaction, stratification after digestion reaction, upper layer suspension, which is put into revolving speed, is
It is centrifuged in 5000r/min centrifuge, collects supernatant, repeated above-mentioned centrifugal process 5 times, collect supernatant, it is spare；
Step 3: the supernatant that step 2 is collected is mixed with NaCl solution, in the centrifuge that revolving speed is 1000r/min
It is centrifuged 30min, discards supernatant liquid, precipitates the acetic acid for being 0.5mol/L with the concentration of 10 times of volumes, later repeated centrifugation 5
It is secondary, it collects supernatant and obtains autologous collagen albumen stoste；
Step 4: crude autologous collagen albumen stoste is mixed with hyaluronic acid solution, citric acid solution, it is centrifuged later simultaneously
Filtering obtains the autologous collagen albumen stoste, and is centrifuged and filtered autologous collagen albumen stoste can be from diameter
It is flowed out in the micropin of 0.5mm；Wherein, the mass percent concentration of the hyaluronic acid solution is 20%, the citric acid solution
Mass percent concentration be 3%.
Embodiment 3
Present embodiment discloses the preparation methods that a kind of cooperation micropin uses autologous collagen albumen stoste, including following step
It is rapid:
It impregnates, is during which kept stirring until at paste Step 1: mature self fibr tissue is added in ultrapure water；
The self fibr tissue of paste is crushed and is put into the ultrapure water of 10 times of volumes, self fibr tissue suspension is obtained, to certainly
The obtained mixture of acetic acid on the rocks in body fibr tissue suspension, and the concentration of mixture is 0.4mg/mL, it is spare；
Step 2: pepsin solution is produced, and the preparation method of pepsin solution includes first weighing pepsin, it
Pepsin is dissolved in the pepsin solution that 10mg/mL is made in 0.5M acetic acid afterwards, is added in the mixture prepared to step 1
Enter pepsin solution and carry out digestion reaction, stratification after digestion reaction, upper layer suspension, which is put into revolving speed, is
It is centrifuged in 4250r/min centrifuge, collects supernatant, repeated above-mentioned centrifugal process 4 times, collect supernatant, it is spare；
Step 3: the supernatant that step 2 is collected is mixed with NaCl solution, in the centrifuge that revolving speed is 2000r/min
It is centrifuged 17.5min, discards supernatant liquid, precipitates the acetic acid for being 0.5mol/L with the concentration of 10 times of volumes, later repeated centrifugation 4
It is secondary, it collects supernatant and obtains autologous collagen albumen stoste；
Step 4: crude autologous collagen albumen stoste is mixed with hyaluronic acid solution, citric acid solution, it is centrifuged later simultaneously
Filtering obtains the autologous collagen albumen stoste, and is centrifuged and filtered autologous collagen albumen stoste can be from diameter
It is flowed out in the micropin of 0.3mm；Wherein, the mass percent concentration of the hyaluronic acid solution is 15%, the citric acid solution
Mass percent concentration be 2%.
Embodiment 4
In taking above-described embodiment 1-3 when any embodiment, the application also proposed a kind of micro- applied to the cooperation
Needle uses the micropin of autologous collagen albumen stoste.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. the preparation method that a kind of cooperation micropin uses autologous collagen albumen stoste, which comprises the following steps:
It impregnates, is during which kept stirring until at paste Step 1: mature self fibr tissue is added in ultrapure water；It will paste
The self fibr tissue of shape is crushed and is put into ultrapure water, obtains self fibr tissue suspension, is suspended to self fibr tissue
The obtained mixture of acetic acid on the rocks, spare in liquid；
Step 2: pepsin solution progress digestion reaction is added in the mixture prepared to step 1, after digestion reaction
Upper layer suspension is put into a centrifuge centrifugation by stratification, collects supernatant, spare；
Step 3: the supernatant that step 2 is collected is mixed with NaCl solution, it is centrifuged later, collects supernatant and obtain slightly
Autologous collagen albumen stoste processed；
Step 4: crude autologous collagen albumen stoste is mixed with hyaluronic acid solution, citric acid solution, it is centrifuged and filters later
Obtain the autologous collagen albumen stoste.
2. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: step
The self fibr tissue of paste is crushed and is put into the ultrapure water of 10 times of volumes in one.
3. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: to certainly
The concentration of mixture made from acetic acid on the rocks is 0.4mg/mL in body fibr tissue suspension.
4. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: step
The preparation method of pepsin solution includes first weighing pepsin in two, pepsin is dissolved in 0.5M acetic acid being made later
The pepsin solution of 10mg/mL.
5. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: described
The step of two include upper layer suspension is put into revolving speed be 3500-5000r/min centrifuge in be centrifuged, collect supernatant, weight
Multiple above-mentioned centrifugal process 3-5 times, supernatant is collected, it is spare.
6. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: described
The step of three include by step 2 collect supernatant and NaCl solution mix by weight ratio, revolving speed be 3000-1000r/
It is centrifuged 5-30min in the centrifuge of min, discards supernatant liquid, precipitating acetic acid, repeated centrifugation 3-5 times later, in collection
Clear liquid obtains crude autologous collagen albumen stoste.
7. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 6, it is characterised in that: precipitating
The acetic acid that concentration with 10 times of volumes is 0.5mol/L.
8. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: step
The mass percent concentration of hyaluronic acid solution described in four is 10-20%, the mass percent concentration of the citric acid solution
For 1-3%.
9. cooperation micropin uses the preparation method of autologous collagen albumen stoste according to claim 1, it is characterised in that: centrifugation
And filtered autologous collagen albumen stoste can be flowed out from the micropin that diameter is 0.1-0.5mm.
10. one kind is applied to the micropin that cooperation micropin described in any one of claim 1-9 uses autologous collagen albumen stoste.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110635836.9A CN113350218A (en) | 2018-10-31 | 2018-10-31 | Autologous collagen stock solution matched with microneedle for use and application thereof |
| CN201811283686.4A CN109276472A (en) | 2018-10-31 | 2018-10-31 | A kind of cooperation micropin uses the preparation method and application of autologous collagen albumen stoste |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811283686.4A CN109276472A (en) | 2018-10-31 | 2018-10-31 | A kind of cooperation micropin uses the preparation method and application of autologous collagen albumen stoste |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110635836.9A Division CN113350218A (en) | 2018-10-31 | 2018-10-31 | Autologous collagen stock solution matched with microneedle for use and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109276472A true CN109276472A (en) | 2019-01-29 |
Family
ID=65174623
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110635836.9A Withdrawn CN113350218A (en) | 2018-10-31 | 2018-10-31 | Autologous collagen stock solution matched with microneedle for use and application thereof |
| CN201811283686.4A Pending CN109276472A (en) | 2018-10-31 | 2018-10-31 | A kind of cooperation micropin uses the preparation method and application of autologous collagen albumen stoste |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110635836.9A Withdrawn CN113350218A (en) | 2018-10-31 | 2018-10-31 | Autologous collagen stock solution matched with microneedle for use and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN113350218A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024117132A1 (en) * | 2022-11-28 | 2024-06-06 | コスメディ製薬株式会社 | Collagen microneedle preparation |
-
2018
- 2018-10-31 CN CN202110635836.9A patent/CN113350218A/en not_active Withdrawn
- 2018-10-31 CN CN201811283686.4A patent/CN109276472A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024117132A1 (en) * | 2022-11-28 | 2024-06-06 | コスメディ製薬株式会社 | Collagen microneedle preparation |
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| Publication number | Publication date |
|---|---|
| CN113350218A (en) | 2021-09-07 |
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Application publication date: 20190129 |
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