CN109275569A - A kind of method for building up of tealeaves regenerating system - Google Patents
A kind of method for building up of tealeaves regenerating system Download PDFInfo
- Publication number
- CN109275569A CN109275569A CN201811429486.5A CN201811429486A CN109275569A CN 109275569 A CN109275569 A CN 109275569A CN 201811429486 A CN201811429486 A CN 201811429486A CN 109275569 A CN109275569 A CN 109275569A
- Authority
- CN
- China
- Prior art keywords
- culture
- tealeaves
- days
- illumination
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method for building up of tealeaves regenerating system, tealeaves is low using conventional method breeding coefficient, and growth cycle is long, while the influence vulnerable to factors such as external environmental conditions.And the method for using tissue cultures, breeding coefficient are high, can carry out selection in any growth phase and expand numerous, the condition that breeding and industrial seedling rearing to tealeaves excellent variety are provided convenience.The present invention is using tealeaves as explant, by differentiation culture, Multiplying culture, culture of rootage, acclimatization and transplants and etc. obtain tealeaves Regeneration in Vitro plant, establish tealeaves regenerating system, solid foundation is established for industrial seedling rearing from now on and domesticating and cultivating, to solve the problems, such as and introduction and acclimatization difficulty is high.
Description
Technical field
The present invention relates to the methods of Plant Tissue Breeding in agricultural biotechnologies, specifically, being related to a kind of tealeaves regeneration
The method for building up of system.
Background technique
Tealeaves is plant of theaceae, the dried leaf of tea, the ground such as main product Jiangsu, wash rice river, Jiangxi, Hubei.Plantation 3 years or more
Tea tree can picking leaves, it is best to pick its tender shoots when having given birth to 4 ~ 5 leaf with sprouting before and after Clear and Bright.Gas is fragrant, bitter and it is puckery.With refresh oneself,
Relieving thirst and restlessness, resolving sputum help digestion, removing toxic substances.Headache is controlled, restlessness and thirst, dyspepsia phlegm is stagnant, dysentery.As people develop the increasing of dynamics, plant
The quantity of species group sharply declines.Therefore some necessary safeguard measures and method should be taken, to safeguard Wild ornamental resources
Quantity.The wild resource of tealeaves is extremely limited, and people arbitrarily pick and the excavation of blindness in recent years, make its quantity by
Serious destruction.It is main to be bred using the modes such as sowing, cuttage, grafting, but breeding coefficient is low, and growth cycle is long, simultaneously
Influence vulnerable to factors such as external environmental conditions.And the method for using tissue cultures, breeding coefficient are high, it can be in any growth step
Numerous, the condition that breeding and industrial seedling rearing to excellent variety are provided convenience is expanded in Duan Jinhang selection.The present invention is outer with tealeaves
Implant, by differentiation culture, Multiplying culture, culture of rootage, acclimatization and transplants and etc. obtain tealeaves Regeneration in Vitro plant, foundation
Tealeaves regenerating system, for from now on industrial seedling rearing and domesticating and cultivating establish solid foundation.
Summary of the invention
The purpose of the present invention is to provide using tealeaves as explant, by breaking up culture, Multiplying culture, culture of rootage, refining
Transplantation of seedlings and etc. obtain tealeaves Regeneration in Vitro plant, tealeaves regenerating system is established, to realize a kind of regeneration of tealeaves
The method for building up of system.
A kind of method for building up of tealeaves regenerating system of the invention, includes the following steps:
Step 1, explant sterilizes: acquisition tealeaves healthy plant delicacy blade dips in washing powder water with banister brush and gently scrubs, originally
Water rinses 34h and is placed in superclean bench, is first washed 5 times with after 75% ethanol disinfection 15s with sterile, then molten with 0.1% mercuric chloride
Liquid disinfectant 8min is dried with aseptic filter paper spare after the droplet on surface again with aseptic water washing 6 times；
Step 2, differentiation culture: it will cut off, be rowed dry on blade with sterile scalpel several through step 1 treated blade petiole
Knife is inoculated in differential medium in such a way that leaf back is contacted with culture medium and carries out callus and inducing clumping bud, inoculation
It is first dark culture 30 days full under the conditions of 29 DEG C afterwards, it is subsequently placed in daily illumination 16 hours, intensity of illumination 4000lx is placed in culture
Temperature counts callus induction rate after cultivating 25 days under conditions of being 29 DEG C, counts differentiation rate after 40 days；Differential medium are as follows:
MS+0.6mg/L TDZ+1.1mg/L IBA+30g/L sucrose+6.0g/L agar, pH 5.8；
Step 3, the callus with Multiple Buds that step 2 induces differentiation to generate Multiplying culture: is divided into 0.8cm's or so
Fritter is simultaneously inoculated into squamous subculture culture medium progress Multiplying culture, first dark culture 9 days full under the conditions of 29 DEG C after inoculation, then sets
In daily illumination 16 hours, intensity of illumination 3000lx, cultivation temperature was long with adventitious bud after cultivating 50 days under conditions of being 29 DEG C
Degree >=1.1cm is that effective bud counts proliferation times；Subculture medium are as follows: MS+1.1mg/L TDZ+0.6mg/L IBA+30g/L sugarcane
Sugar+6.1g/L agar, pH 5.8；
Step 4, culture of rootage: the consistent tissue-cultured seedling of 3 growth conditions of selecting step is cut into the stem section of 4cm long and is seeded to and takes root
Root induction in culture, inoculation are placed on daily illumination 16 hours, intensity of illumination 5000lx, the condition that cultivation temperature is 29 DEG C
Lower culture observes the upgrowth situation of seedling and counts rooting rate after 40 days；Root media are as follows: 1/2MS+3.5mg/L IBA+
2.1mg/L ABT1+ 30g/L sucrose+6.0g/L agar, pH 5.8；
Step 5, acclimatization and transplants: choosing after culture of rootage, and the tissue-cultured seedling of well developed root system and plant strain growth stalwartness carries out hardening shifting
Plant, wash away the basifixed agar of tissue-cultured seedling first, be transplanted in the river sand matrix irrigated in advance later, backward seedling
Be sprayed, build simple plastic canopy, guarantee in canopy relative air humidity up to 99%, plastic covering film, to guarantee the hardening phase
Between humidity in canopy, gradually lift film ventilation and penetrating light later and pay attention to the humidity in canopy, after sand culture 25 days, be transplanted to by turf
Soil: fertile soil: in the mixed-matrix of perlite=4:1:1 composition, sprinkling profoundly water after plant, and when routine servicing pays attention to guaranteeing that basin soil is wet
Profit avoids ponding, and sprays water often to blade, and transplanting counted transplanting survival rate after 20 days.
Compared with prior art the invention has the advantages that breeding coefficient is high, selection expansion can be carried out in any growth phase
Condition numerous, that breeding and industrial seedling rearing to excellent variety are provided convenience.By breaking up culture, Multiplying culture, training of taking root
Support, acclimatization and transplants and etc. obtain Regeneration in Vitro plant, regenerating system is established, for industrial seedling rearing and domesticating and cultivating from now on
Solid foundation is established, to solve the problems, such as that introduction and acclimatization difficulty is high.
Specific embodiment
It the following examples are further illustrations of the invention, is not limitation of the present invention.
Embodiment 1:
(1) explant sterilizes: acquisition tealeaves healthy plant delicacy blade dips in washing powder water with banister brush and gently scrubs, tap water
It rinses 5h to be placed in superclean bench, first be washed 7 times with after 75% ethanol disinfection 8s with sterile, then disappeared with 0.1% mercuric chloride solution
Malicious 8min is dried with aseptic filter paper spare after the droplet on surface again with aseptic water washing 8 times.
(2) differentiation culture: it will cut off through step (1) treated blade petiole, be rowed dry on blade with sterile scalpel
Several knives are inoculated in differential medium in such a way that leaf back is contacted with culture medium and carry out callus and inducing clumping bud.It connects
It is first dark culture 20 days full under the conditions of 26 DEG C after kind, it is subsequently placed in daily illumination 12 hours, intensity of illumination 3000lx is placed in training
Statistics differentiation rate is 98% after statistics callus induction rate is 97%, 40 days after feeding temperature is cultivated 25 days under conditions of being 26 DEG C.
The differential medium are as follows: MS+1.6mg/L TDZ+0.4mg/L IBA+26g/L sucrose+4.5g/L agar, pH 5.7.
(3) Multiplying culture: it is divided into 0.8cm left the callus with Multiple Buds that step (2) induce differentiation to generate
Right fritter is simultaneously inoculated into squamous subculture culture medium progress Multiplying culture.It is first dark culture 7 days full under the conditions of 26 DEG C after inoculation, so
Be placed on daily illumination 13 hours, intensity of illumination 3000lx, cultivation temperature be 26 DEG C under conditions of cultivate 52 days after with indefinite
Bud length >=1.1cm is that effective bud statistics proliferation times are 38.The subculture medium are as follows: MS+0.6mg/L TDZ+
0.2mg/L IBA+40g/L sucrose+5g/L agar, pH 5.7.
(4) culture of rootage: the consistent tissue-cultured seedling of selecting step (3) growth conditions is cut into the stem section of 7cm long and is seeded to life
Root induction in root culture.Inoculation is placed on daily illumination 16 hours, intensity of illumination 3500lx, the item that cultivation temperature is 26 DEG C
Rooting rate 95% after being cultivated 40 days under part.The root media are as follows: 1/2MS+1.6mg/L IBA+1.1mg/L ABT1+
20g/L sucrose+3.6g/L agar, pH 5.7.
(5) acclimatization and transplants: choosing after culture of rootage, and the tissue-cultured seedling of well developed root system and plant strain growth stalwartness carries out hardening shifting
Plant, wash away the basifixed agar of tissue-cultured seedling first, be transplanted in the river sand matrix irrigated in advance later, backward seedling
Be sprayed, build simple plastic canopy, guarantee in canopy relative air humidity up to 99%, plastic covering film, to guarantee the hardening phase
Between humidity in canopy, gradually lift film ventilation and penetrating light later and pay attention to the humidity in canopy, after sand culture 15 days, be transplanted to by turf
Soil: fertile soil: in the mixed-matrix of perlite=4:1:1 composition, around sprinkling profoundly water after plant, when routine servicing, pays attention to guaranteeing basin soil
It is wet to avoid ponding, and spray water often to blade, statistics transplanting survival rate is 95% after transplanting 21 days.
Embodiment 2:
(1) explant sterilizes: acquisition tealeaves healthy plant delicacy blade dips in washing powder water with banister brush and gently scrubs, tap water
It rinses 6h to be placed in superclean bench, first be used sterile water 12 times with after 75% ethanol disinfection 10s, then disappeared with 0.1% mercuric chloride solution
Malicious 10min is dried with aseptic filter paper spare after the droplet on surface again with aseptic water washing 8 times.
(2) differentiation culture: it will cut off through step (1) treated blade petiole, be rowed dry on blade with sterile scalpel
Several knives are inoculated in differential medium in such a way that leaf back is contacted with culture medium and carry out callus and inducing clumping bud.It connects
It is first dark culture 25 days full under the conditions of 28 DEG C after kind, it is subsequently placed in daily illumination 18 hours, intensity of illumination 3500lx is placed in training
Statistics differentiation rate is 92% after statistics callus induction rate is 95%, 41 days after feeding temperature is cultivated 28 days under conditions of being 28 DEG C.
The differential medium are as follows: MS+2.1mg/L TDZ+0.1mg/L IBA+30g/L sucrose+6g/L agar, pH 5.9.
(3) Multiplying culture: it is divided into 0.8cm left the callus with Multiple Buds that step (2) induce differentiation to generate
Right fritter is simultaneously inoculated into squamous subculture culture medium progress Multiplying culture.It is first dark culture 9 days full under the conditions of 28 DEG C after inoculation, so
Be placed on daily illumination 15 hours, intensity of illumination 4000lx, cultivation temperature be 28 DEG C under conditions of cultivate 51 days after with indefinite
Bud length >=1.1cm is that effective bud statistics proliferation times are 34.The subculture medium are as follows: MS+1.1mg/L TDZ+
0.2mg/LIBA+31g/L sucrose+4.9g/L agar, pH 5.9.
(4) culture of rootage: the consistent tissue-cultured seedling of selecting step (3) growth conditions is cut into the stem section of 5cm long and is seeded to life
Root induction in root culture.Inoculation is placed on daily illumination 16 hours, intensity of illumination 3600lx, the item that cultivation temperature is 28 DEG C
Rooting rate 96% after being cultivated 40 days under part.The root media are as follows: 1/2MS+1.9mg/L IBA+1.1mg/L ABT1+
31g/L sucrose+5.6g/L agar, pH 5.9.
(5) acclimatization and transplants: choosing after culture of rootage, and the tissue-cultured seedling of well developed root system and plant strain growth stalwartness carries out hardening shifting
Plant, wash away the basifixed agar of tissue-cultured seedling first, be transplanted in the river sand matrix irrigated in advance later, backward seedling
Be sprayed, build simple plastic canopy, guarantee in canopy relative air humidity up to 99%, plastic covering film, to guarantee the hardening phase
Between humidity in canopy, gradually lift film ventilation and penetrating light later and pay attention to the humidity in canopy, after sand culture 22 days, be transplanted to by turf
Soil: fertile soil: in the mixed-matrix of perlite=4:2:1 composition, around sprinkling profoundly water after plant, when routine servicing, pays attention to guaranteeing basin soil
It is wet to avoid ponding, and spray water often to blade, transplanting survival rate 97% is counted after transplanting 19 days.
Claims (1)
1. a kind of method for building up of tealeaves regenerating system, it is characterised in that the following steps are included:
Step 1, explant sterilizes: acquisition tealeaves healthy plant delicacy blade dips in washing powder water with banister brush and gently scrubs, originally
Water rinses 34h and is placed in superclean bench, is first washed 5 times with after 75% ethanol disinfection 15s with sterile, then molten with 0.1% mercuric chloride
Liquid disinfectant 8min is dried with aseptic filter paper spare after the droplet on surface again with aseptic water washing 6 times；
Step 2, differentiation culture: it will cut off, be rowed dry on blade with sterile scalpel several through step 1 treated blade petiole
Knife is inoculated in differential medium in such a way that leaf back is contacted with culture medium and carries out callus and inducing clumping bud, inoculation
It is first dark culture 30 days full under the conditions of 29 DEG C afterwards, it is subsequently placed in daily illumination 16 hours, intensity of illumination 4000lx is placed in culture
Temperature counts callus induction rate after cultivating 25 days under conditions of being 29 DEG C, counts differentiation rate after 40 days；Differential medium are as follows:
MS+0.6mg/L TDZ+1.1mg/L IBA+30g/L sucrose+6.0g/L agar, pH 5.8；
Step 3, the callus with Multiple Buds that step 2 induces differentiation to generate Multiplying culture: is divided into 0.8cm's or so
Fritter is simultaneously inoculated into squamous subculture culture medium progress Multiplying culture, first dark culture 9 days full under the conditions of 29 DEG C after inoculation, then sets
In daily illumination 16 hours, intensity of illumination 3000lx, cultivation temperature was long with adventitious bud after cultivating 50 days under conditions of being 29 DEG C
Degree >=1.1cm is that effective bud counts proliferation times；Subculture medium are as follows: MS+1.1mg/L TDZ+0.6mg/L IBA+30g/L sugarcane
Sugar+6.1g/L agar, pH 5.8；
Step 4, culture of rootage: the consistent tissue-cultured seedling of 3 growth conditions of selecting step is cut into the stem section of 4cm long and is seeded to and takes root
Root induction in culture, inoculation are placed on daily illumination 16 hours, intensity of illumination 5000lx, the condition that cultivation temperature is 29 DEG C
Lower culture observes the upgrowth situation of seedling and counts rooting rate after 40 days；Root media are as follows: 1/2MS+3.5mg/L IBA+
2.1mg/L ABT1+ 30g/L sucrose+6.0g/L agar, pH 5.8；
Step 5, acclimatization and transplants: choosing after culture of rootage, and the tissue-cultured seedling of well developed root system and plant strain growth stalwartness carries out hardening shifting
Plant, wash away the basifixed agar of tissue-cultured seedling first, be transplanted in the river sand matrix irrigated in advance later, backward seedling
Be sprayed, build simple plastic canopy, guarantee in canopy relative air humidity up to 99%, plastic covering film, to guarantee the hardening phase
Between humidity in canopy, gradually lift film ventilation and penetrating light later and pay attention to the humidity in canopy, after sand culture 25 days, be transplanted to by turf
Soil: fertile soil: in the mixed-matrix of perlite=4:1:1 composition, sprinkling profoundly water after plant, and when routine servicing pays attention to guaranteeing that basin soil is wet
Profit avoids ponding, and sprays water often to blade, and transplanting counted transplanting survival rate after 20 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811429486.5A CN109275569A (en) | 2018-11-27 | 2018-11-27 | A kind of method for building up of tealeaves regenerating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811429486.5A CN109275569A (en) | 2018-11-27 | 2018-11-27 | A kind of method for building up of tealeaves regenerating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109275569A true CN109275569A (en) | 2019-01-29 |
Family
ID=65173358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811429486.5A Pending CN109275569A (en) | 2018-11-27 | 2018-11-27 | A kind of method for building up of tealeaves regenerating system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109275569A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110383993A (en) * | 2019-06-20 | 2019-10-29 | 云南省农业科学院花卉研究所 | A kind of efficient cuttage breeding method of camellia simple bud |
| CN111972292A (en) * | 2020-09-08 | 2020-11-24 | 陕西理工大学 | Purple bud tea cultivation method |
| CN115623985A (en) * | 2022-08-18 | 2023-01-20 | 山东农业大学 | Method for culturing red callus of tea tree |
| CN117796321A (en) * | 2023-12-29 | 2024-04-02 | 云南省农业科学院茶叶研究所 | Culture medium for improving survival rate of tea tree tissue culture seedlings |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008090474A2 (en) * | 2007-01-24 | 2008-07-31 | University Of Guelph | Production of rosmarinic acid from spearmint and uses thereof |
| CN104686332A (en) * | 2015-02-22 | 2015-06-10 | 刘木娇 | Method for establishing tissue culture regeneration system of camellia sinensis L. |
| CN104737907A (en) * | 2015-03-06 | 2015-07-01 | 朱远星 | Building method of rhododendron aureum leaf regeneration system |
| CN104756867A (en) * | 2015-03-31 | 2015-07-08 | 中国林业科学研究院亚热带林业研究所 | Establishing method of camellia changii leaf callus regeneration system |
| CN105494107A (en) * | 2016-02-25 | 2016-04-20 | 湖南农业大学 | Tea tree tissue culture method |
| CN108834900A (en) * | 2018-07-31 | 2018-11-20 | 安徽农业大学 | Inhibit the cultural method of explant oxidizing brown stain and endophytic bacterial contamination in tea-tree tissue culture |
-
2018
- 2018-11-27 CN CN201811429486.5A patent/CN109275569A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008090474A2 (en) * | 2007-01-24 | 2008-07-31 | University Of Guelph | Production of rosmarinic acid from spearmint and uses thereof |
| CN104686332A (en) * | 2015-02-22 | 2015-06-10 | 刘木娇 | Method for establishing tissue culture regeneration system of camellia sinensis L. |
| CN104737907A (en) * | 2015-03-06 | 2015-07-01 | 朱远星 | Building method of rhododendron aureum leaf regeneration system |
| CN104756867A (en) * | 2015-03-31 | 2015-07-08 | 中国林业科学研究院亚热带林业研究所 | Establishing method of camellia changii leaf callus regeneration system |
| CN105494107A (en) * | 2016-02-25 | 2016-04-20 | 湖南农业大学 | Tea tree tissue culture method |
| CN108834900A (en) * | 2018-07-31 | 2018-11-20 | 安徽农业大学 | Inhibit the cultural method of explant oxidizing brown stain and endophytic bacterial contamination in tea-tree tissue culture |
Non-Patent Citations (2)
| Title |
|---|
| 曹丹等: "茶树组织培养研究进展", 《安徽农业科学》 * |
| 田奥磊等: "茶树离体培养类型及其应用研究进展", 《河南农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110383993A (en) * | 2019-06-20 | 2019-10-29 | 云南省农业科学院花卉研究所 | A kind of efficient cuttage breeding method of camellia simple bud |
| CN111972292A (en) * | 2020-09-08 | 2020-11-24 | 陕西理工大学 | Purple bud tea cultivation method |
| CN115623985A (en) * | 2022-08-18 | 2023-01-20 | 山东农业大学 | Method for culturing red callus of tea tree |
| CN117796321A (en) * | 2023-12-29 | 2024-04-02 | 云南省农业科学院茶叶研究所 | Culture medium for improving survival rate of tea tree tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109275569A (en) | A kind of method for building up of tealeaves regenerating system | |
| CN103155809B (en) | Method of culturing tomato grafted seedling | |
| CN104126506B (en) | A kind of method for tissue culture of American Red rocket Lagerstroemia indica L. | |
| CN109392719A (en) | A kind of method for building up of madder regenerating system | |
| CN102715092B (en) | Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches | |
| CN108157180A (en) | A kind of method of the open industrial fast breeding of potato virus-free plantlet | |
| CN112335549A (en) | Method for obtaining larch regeneration plant through tissue in-vitro culture | |
| CN104041418A (en) | Method for generating somatic embryos of catalpa bungei through induction | |
| CN106538382B (en) | Method for establishing efficient eremochloa ophiuroides regeneration system by taking young ears as explants | |
| CN103460971A (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN103583357B (en) | Method for sterile seeding of lithops and establishing regeneration system | |
| CN102487829A (en) | Method of comprehensive detoxification and rapid propagation for starch-type water chestnut | |
| CN106172011B (en) | A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method | |
| CN106258976B (en) | A kind of tissue culturing fast seedling-cultivating method of mustard type rape | |
| CN105766636B (en) | A kind of peony tissue culture regeneration method | |
| CN105900837B (en) | A kind of method for cultivating fast breeding iron orchid species seedling by suspending | |
| CN104686334A (en) | Tissue culture and rapid propagation method for androsace longifolia | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN103704140B (en) | A kind of with children spend into Explants In Tissue Culture breeding polyploid royal paulownia method | |
| CN110999782A (en) | Tissue culture and rapid propagation method for gardenia jasminoides | |
| CN103798139B (en) | A kind of is the method that outer implant sets up Bulbus Lilii embryo callus subculture regenerating system with petal | |
| CN105409773A (en) | Lophophora williamsii sterile seeding and regeneration system establishing method | |
| CN105145358A (en) | Tissue culture and rapid propagation method for common fibraurea stem | |
| CN104488718A (en) | Quick propagation method for oreocharis flavida | |
| CN106069774B (en) | A kind of sinocalamus latiflorus stem end evoked callus and the method for obtaining regeneration plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190129 |
|
| WD01 | Invention patent application deemed withdrawn after publication |