CN109266692A - A kind of biogas slurry pretreatment corn stover natural pond method and its condition optimizing method - Google Patents
A kind of biogas slurry pretreatment corn stover natural pond method and its condition optimizing method Download PDFInfo
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- CN109266692A CN109266692A CN201811195826.2A CN201811195826A CN109266692A CN 109266692 A CN109266692 A CN 109266692A CN 201811195826 A CN201811195826 A CN 201811195826A CN 109266692 A CN109266692 A CN 109266692A
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Abstract
The present invention provides a kind of biogas slurries to pre-process corn stover natural pond method, the following steps are included: air-drying and pulverizing and sieving to corn stover first, then it carries out pretreatment biogas slurry and is inoculated with the preparation of biogas slurry, then corn stover is pre-processed by pre-processing biogas slurry, pretreated corn stover and inoculation biogas slurry is finally subjected to anaerobic digestion.The present invention also provides the condition optimizing methods to natural pond method processed, that is, to pre-process biogas slurry dosage, pretreatment temperature and pretreatment time as the single factor test of condition optimizing, obtain the optimal conditions of preprocessing process by response surface experiments design and result.The present invention is by pre-processing lignin and the higher corn of content of cellulose using biogas slurry as promotor, so that pretreated corn be made to be easy to carry out anaerobic fermentation；Simultaneously in operation without equipment especially, cost is relatively low；And pretreated optimum condition 19.08%TS is obtained by response phase method, 29.78 DEG C and 5.42 days, obtaining theoretical gas production is 4786.3mL.
Description
Technical field
The present invention relates to bioenergy technical field, specially a kind of biogas slurry pretreatment corn stover natural pond method and its item
Piece optimization method.
Background technique
The stalk yield in China is very big, and the utilization of stalk is very crucial, and wherein biological treatment producing methane can not only be reduced
The many problem of environmental pollutions of stalk surplus bring also can solve the marsh gas raw materials shortage in some areas, therefore biological treatment
Producing methane is widely used.But due to the special structure of stalk itself, the silicon oxygen wax coat on surface, fibrination is to crystallize
State exists, and microorganism can not effectively degrade.Therefore it needs to pre-process stalk raw materials.
Currently used preprocess method mainly includes physics, chemistry and biological three categories.However due to existing pre- place
The disadvantages of equipment requirement existing for reason method is high, the generation of high energy consumption and acidic and alkaline waste water, is not yet industrially answered at present
With.
The method of Biological Pretreatment is some bacteriums of screening, fungi, actinomyces etc., or uses composite bacteria agent, such as black song
Mould (Aspergillus), trichoderma (Trichoderma), penicillium oxalicum (Penicillium) and white-rot fungi composition HK-4 and
Whiterot fungi and trichoderma, the enzymic catalytic reaction occurred under being acted synergistically using a variety of biological enzyme of microorganism secretion, destroy the big of stalk
Molecular substance.Biological Pretreatment is environmental-friendly more to be paid close attention to because the usage amount of its chemistry and the energy is low, but concentrates at present
In the influence in various specified microorganisms microbial inoculums to ligocellulose degradation and gas production.
Biogas slurry is wherein no lack of degradable wood per se with a large amount of microorganism as the substance in anaerobic fermentation system
The microorganism of matter cellulose, but as the waste after anaerobic fermentation, its processing also has certain difficulty to current biogas slurry, if can be by natural pond
Liquid can reduce its discharge as the pretreated raw material of anaerobic fermentation while reduce pretreated cost.Chu Lili etc. is with wheat stalk
For raw material, VS gas production can reach 149.4mL/g after biogas slurry pre-processes 6 days.Hu etc. is that raw material locates biogas slurry in advance with corn stover
Reason optimizes pretreatment time and biogas slurry TS content, and obtaining result is that the biogas slurry through 30.5g/L pre-processes 3 days, reaches
The time of 80% cumulative gas production (t80) can shorten 33.3%.Lee's equality it has been investigated that, through biogas slurry pretreated water rice straw its
TS factor of created gase reaches 333.9mL/g, and TS methane production rate reaches 180.7mL/g, can improve 27.9% and 21.2% compared with blank, simultaneously
Gas cycle time is produced to 19d.But most of research at present is focusing only on determining biogas slurry pretreatment and gas production can be improved, specifically
Optimal conditions in preprocess method and preprocess method is still worth probing into.
Summary of the invention
The purpose of the present invention is to solve the deficiencies of above-mentioned technology, provide a kind of biogas slurry pretreatment corn stover natural pond side
Method and its condition optimizing method, this method mild condition, process is simple, reuses and connects after being pre-processed by biogas slurry to corn stover
Kind biogas slurry and pretreated corn stover anaerobic digestion, can effectively improve gas production, and using response phase method to natural pond mistake processed
The pretreatment condition of journey optimizes, and obtains optimal pretreatment condition.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of biogas slurry pretreatment corn stover natural pond method, comprising the following steps:
Step 1, corn stalk raw material prepare
Corn stover is air-dried and crushed, is saved backup；
Step 2, biogas slurry preparation
Pretreatment biogas slurry: it is then mixed to gas is no longer produced within the biogas slurry standing sedimentation that batch-type dry fermentation is generated 3~7 days
It is uniformly 0.37% ± 0.11%, pH 7.2 to TS concentration, obtains pretreatment biogas slurry, it is spare；
Inoculation biogas slurry: the biogas slurry standing sedimentation 7~8 days in continuous stirred tank reactor is so incensed that be inoculated with natural pond to no longer producing
The TS concentration of liquid, the inoculation biogas slurry is 4.36% ± 0.04%, pH 7.1；
Step 3, preprocess method
It weighs corn stover several pieces to be respectively placed in sealing container, is separately added into pretreatment biogas slurry that step 2 obtains simultaneously
Mixing pre-processes 2~10 so that the TS concentration of mixture is 10%~30% under 20~50 DEG C of constant temperatures after mixing evenly
It；
Step 4, anaerobic digestion
Corn stover pretreated in step 3 is mixed with the inoculation biogas slurry that step 2 obtains according to the mass ratio of 1:4
It is even, and being diluted with water to TS concentration is 8%, is sealed under 35 ± 1 DEG C of constant temperature and carries out anaerobic digestion.
Preferably, 3mm sieve is crossed in step 1 after corn straw smashing.
Preferably, the mass ratio of corn stover and pretreatment biogas slurry is 1:1.82~7.66 in step 3.
Preferably, the reactor of anaerobic digestion includes by the graduated cylinder of blue the lid reagent bottle and a 1L of two 1L in step 4
With a set of gas connection device of emulsion tube connection composition, the blue lid reagent bottle of two 1L is respectively as the digestion bottle of raw material, natural pond
Gas gas bottle, the graduated cylinder of 1L is as the graduated cylinder that catchments.
The condition optimizing method of biogas slurry pretreatment corn stover natural pond method in the present invention, comprising the following steps:
S1, it determines single factor test, and transcoding, coding transform is carried out to it
Using pretreatment biogas slurry dosage, pretreatment temperature and pretreatment time as the single factor test of condition optimizing, then divide
Not Gai Bian single factor test according to biogas slurry pretreatment corn stover natural pond method carry out natural pond processed, the biogas collected simultaneously counts each Dan Yin
The gas production of element, obtains the maximum each single factor test numerical value of gas production, establishes experimental factor level code table；
S2, testing program is determined using center combination design CCD principle
The experimental factor level code table obtained according to S1, selection pretreatment biogas slurry dosage, pretreatment temperature and pre- place
Reason three single factor tests of time are independent variable, using gas production as response, with center combination design CCD principle, carry out response surface
Experimental design；
S3, it completes to test according to testing program
According to the pretreatment for carrying out corn stover in the response surface experiments scheme of S2 design, pretreated corn stalk is used
Stalk natural pond, the biogas collected, and count；
S4, response surface model is established, analyzes test result
The test data input response surface software that S3 is obtained carries out regression analysis, is analyzed according to software as a result, obtaining single
The regression relation of factor and response obtains response surface three-dimensional figure and judges influence situation of each factor to response, finally
Horizontal parameters combination after determining each factors optimization.
Response surface software is 8.06 software of Design-Expert in S4.
Compared with prior art, beneficial effects of the present invention are as follows:
1, the method in biogas slurry pretreatment provided by the invention corn stover natural pond is easy to operate, right using biogas slurry as promotor
Lignin and the higher corn of content of cellulose are pre-processed, so that pretreated corn be made to be easy to carry out anaerobic fermentation；
Simultaneously in operation without equipment especially, cost is relatively low；
2, method provided by the invention can effectively increase gas production, and gas producing efficiency significantly improves；
3, the present invention optimizes the pretreatment condition in natural pond method processed by response phase method, obtains pretreated best
Condition is pretreatment biogas slurry dosage 19.08%TS, and 29.78 DEG C of pretreatment temperature, pretreatment time 5.42 days, theoretical gas production was
4786.3mL。
Detailed description of the invention
Fig. 1 be 1- of embodiment of the present invention embodiment 5 in pretreatment front and back corn stover lignocellulosic contain spirogram；
Fig. 2 is pretreatment front and back corn in the embodiment of the present invention 4, embodiment 6, embodiment 7, embodiment 8 and embodiment 9
The lignocellulosic of stalk contains spirogram；
Fig. 3 is that the present invention is before pre-processing in embodiment 4, embodiment 10, embodiment 11, embodiment 12 and embodiment 13
Afterwards the lignocellulosic of corn stover contain spirogram；
Fig. 4 is the gas production of different pretreatments condition in 1- of embodiment of the present invention embodiment 13 and comparative example；
Fig. 5 is the contour that pretreatment biogas slurry additional amount and pretreatment temperature influence biogas output in the present invention；
Fig. 6 is the response surface figure that pretreatment biogas slurry additional amount and pretreatment temperature influence biogas output in the present invention；
Fig. 7 is the contour that pretreatment temperature and pretreatment time influence biogas output in the present invention；
Fig. 8 is the response surface figure that pretreatment temperature and pretreatment time influence biogas output in the present invention；
Fig. 9 is the gas production of verification test in the present invention.
Specific embodiment
Below by specific embodiment example, the present invention will be described in detail.The scope of the present invention is not limited to the tool
Body embodiment.Corn stover used in the present invention comes from Beijing suburb, with reference to VS in APHA method measurement corn stover
Concentration will cross 40 meshes, with FOSS cellulose analyzer to jade according to Van Soest cellulose measuring principle after sample comminution
The content of cellulose, hemicellulose and lignin is measured in rice stalk；Obtain corn stover TS concentration be 90.39% ±
0.18%, VS concentration are 83.79% ± 0.47%, and the content of cellulose is 33.40% ± 0.62%, and the content of hemicellulose is
22.89% ± 0.63%, the content of lignin is 9.41% ± 0.43%.
Embodiment 1
The method for present embodiments providing a kind of biogas slurry pretreatment corn stover natural pond, comprising the following steps:
Step 1, corn stalk raw material prepare
Corn stover is air-dried and crushed, 3mm sieve is crossed, saves backup；
Step 2, biogas slurry preparation
Pretreatment biogas slurry: it is derived from the biogas slurry that the batch-type dry fermentation of laboratory progress generates, batch-type dry fermentation is generated
Biogas slurry standing sedimentation 7 days to gas is no longer produced, being then uniformly mixed to TS concentration is 0.37% ± 0.11%, pH 7.2, is obtained
Biogas slurry is pre-processed, it is spare；
Inoculation biogas slurry: being derived from the biogas slurry stablized in the continuous stirred tank reactor for producing gas, will be in continuous stirred tank reactor
Biogas slurry standing sedimentation 7 days be so incensed that be inoculated with biogas slurry to no longer producing, the TS concentration of the inoculation biogas slurry is 4.36% ± 0.04%,
PH is 7.1；
Step 3, preprocess method
It weighs corn stover to be placed in sealing container, the pretreatment biogas slurry that step 2 obtains, which is added, keeps the TS of mixture dense
Degree is 20%, i.e. pretreatment biogas slurry dosage is 20%TS, is pre-processed 6 days under 20 DEG C of constant temperatures after mixing evenly；
Step 4, anaerobic digestion
The blue lid that the inoculation biogas slurry that corn stover pretreated in 50g step 3 and 200g step 2 obtain is added to 1L is tried
In agent bottle, and it is diluted with water to 900mL thereto, is then sealed and placed in progress anaerobism in 35 ± 1 DEG C of isothermal reactor and disappears
Change, the blue lid reagent bottle for reusing 1L is used as the graduated cylinder that catchments as the graduated cylinder of biogas gas bottle and 1L, will fill in biogas gas bottle
Full water, and gas connection device is formed using emulsion tube connection above-mentioned apparatus.
Embodiment 2
It is identical as the natural pond method processed of embodiment 1, the difference is that the pretreatment temperature for pre-processing corn stover in step 3 is 25
℃。
Embodiment 3
It is identical as the natural pond method processed of embodiment 1, the difference is that the pretreatment temperature for pre-processing corn stover in step 3 is 30
℃。
Embodiment 4
It is identical as the natural pond method processed of embodiment 1, the difference is that the pretreatment temperature for pre-processing corn stover in step 3 is 35
℃。
Embodiment 5
It is identical as the natural pond method processed of embodiment 1, the difference is that the pretreatment temperature for pre-processing corn stover in step 3 is 40
℃。
Embodiment 6
It is identical as the natural pond method processed of embodiment 4, the difference is that the pretreatment time for pre-processing corn stover in step 3 is 2
It.
Embodiment 7
It is identical as the natural pond method processed of embodiment 4, the difference is that the pretreatment time for pre-processing corn stover in step 3 is 4
It.
Embodiment 8
It is identical as the natural pond method processed of embodiment 4, the difference is that the pretreatment time for pre-processing corn stover in step 3 is 8
It.
Embodiment 9
It is identical as the natural pond method processed of embodiment 4, the difference is that the pretreatment time for pre-processing corn stover in step 3 is 10
It.
Embodiment 10
It is identical as the natural pond method processed of embodiment 4, adding for biogas slurry is pre-processed when the difference is that pre-processing corn stover in step 3
Entering amount is 10%TS.
Embodiment 11
It is identical as the natural pond method processed of embodiment 4, adding for biogas slurry is pre-processed when the difference is that pre-processing corn stover in step 3
Entering amount is 15%TS.
Embodiment 12
It is identical as the natural pond method processed of embodiment 4, adding for biogas slurry is pre-processed when the difference is that pre-processing corn stover in step 3
Entering amount is 25%TS.
Embodiment 13
It is identical as the natural pond method processed of embodiment 4, adding for biogas slurry is pre-processed when the difference is that pre-processing corn stover in step 3
Entering amount is 35%TS.
Comparative example
Corn stover is air-dried and crushed, 3mm sieve is crossed, 50g corn stover and 200g inoculation biogas slurry are then added to 1L
Blue lid reagent bottle in, and be diluted with water to 900mL thereto, be then sealed and placed in 35 ± 1 DEG C of isothermal reactor into
Row anaerobic digestion, the blue lid reagent bottle for reusing 1L is used as the graduated cylinder that catchments as the graduated cylinder of biogas gas bottle and 1L, by biogas collection
Water is filled in gas cylinder, and forms gas connection device using emulsion tube connection above-mentioned apparatus.
The corn stover of pretreatment front and back in embodiment 1- embodiment 13 is carried out the measurement of wood fibre cellulose content by we,
Continuous mode will cross 40 meshes according to Van Soest cellulose measuring principle after sample comminution, with FOSS cellulose analyzer into
Row measurement, and the drop that calculation formula (formula 1) calculates biogas slurry pretreatment front and back lignocellulosic is led according to ligocellulose degradation
Solution rate, wherein degradation rate is higher shows that pretreating effect is better.
K=(1-S1/S0) * 100% formula 1
In formula: SO is the content of lignocellulosic in stalk, and %, S1 are that lignocellulosic contains in stalk after pre-processing
Amount, %.
Fig. 1 is that the lignocellulosic of pretreatment front and back corn stover contains spirogram in embodiment 1- embodiment 5, that is, in advance
Influence of the treatment temperature to wood fibre cellulose content, it will be seen from figure 1 that for the degradation of cellulose, by different temperatures
Pretreated degradation rate, which carries out pretreatment to corn stover with biogas slurry at 3.82%~9.27%, 30 DEG C and 35 DEG C, can be obtained preferably
Result.For degradation rate through biogas slurry pretreatment hemicellulose at different temperatures 13.05%~18.66%, degradation effect is bright
It is aobvious.But for lignin, Lignin degradation rate is 5.10%~7.44%.From the point of view of wood fibre cellulose content, at 30 DEG C
The content of lignocellulosic drops to 63.44% from 72.41%, and degradation rate has reached 12.39%.
Fig. 2 is pretreatment front and back corn stover in embodiment 4, embodiment 6, embodiment 7, embodiment 8 and embodiment 9
Lignocellulosic contains spirogram, that is, influence of the pretreatment time to wood fibre cellulose content, figure it is seen that by
After pretreatment in 10 days, cellulose degradation rate reaches 10.22%, and hemicellulose level is wooden from 26.52% degradation to 21.26%
The degradation rate of element reaches 7.86%.With the extension of pretreatment time, the straw lignocellulose component infiltrated by biogas slurry is swollen
Swollen, each ingredient degradation rate constantly increases in lignocellulosic, although reducing from degradation rate amplitude of variation after 6 days, i.e., with 6 days
Degradation afterwards tends towards stability.
Fig. 3 is pretreatment front and back corn stalk in embodiment 4, embodiment 10, embodiment 11, embodiment 12 and embodiment 13
The lignocellulosic of stalk contains spirogram, that is, influence of the pretreatment biogas slurry additional amount to wood fibre cellulose content, can be with from Fig. 3
Find out, as the degradation rate of each ingredient of raising (i.e. the reduction of biogas slurry dosage) lignocellulosic of TS gradually decreases.Cellulose drop
Solution rate is 6.72%~9.68%, and the degradation rate of lignin is 1.91%~7.75%, and the degradation effect of hemicellulose is obvious
8.26%~20.55%.The content of cellulose handled by the amount that TS is 10% and 15% difference compared with blank control
Significantly.From the point of view of the degradation effect of hemicellulose, the influence that this five levels of selection degrade to it is significant, and is using
When 30%TS, lignocellulosic each component content degradation effect is unobvious, may be contacted due to biogas slurry with stalk raw materials endless
Entirely, part material cannot with come into full contact with, cause its degradation effect bad.
Based on same inventive concept, the condition that we additionally provide a kind of biogas slurry pretreatment corn stover natural pond method is excellent
Change method, comprising the following steps:
S1, it determines single factor test, and transcoding, coding transform is carried out to it
The single factor test that biogas slurry dosage will be pre-processed, pretreatment temperature and pretreatment time optimize as pretreatment condition,
Then change single factor test respectively and carry out natural pond processed according to biogas slurry pretreatment corn stover natural pond method, the biogas collected and statistics
The gas production of each single factor test obtains the maximum each single factor test numerical value of gas production, establishes experimental factor level code table；
Table 1 is that ligocellulose degradation leads table under different condition, as can be seen from the table as the temperature rises, wood
The degradation rate of matter cellulose increases and is that degradation rate reaches maximum value at 30 DEG C.There are a large amount of humics in anaerobic dry fermentation biogas slurry
Acid and microorganism, temperature have a great impact to the activity of microorganism, therefore it drops in the different different natures of temperature microorganism
The effect of solution generates difference, at the same time can there is a phenomenon where internal temperatures to increase by the way of such stewing process.And with
The extension ligocellulose degradation of time lead and gradually rise, although day degradation rate increases from 6 days to 10 but its rising tends to
Gently, chosen for the efficiency of degradation pretreatment time 6 days it is more reasonable.For the dosage of biogas slurry, from the experimental results,
It is best for 10% degradation rate for selecting TS content from the point of view of degradation effect, but it is reported that can be allowed with the in the best state of biogas slurry treatment
Corn stover is fully absorbed to flow out without biogas slurry, and excessive biogas slurry will increase the organic loading of anaerobic system, detest subsequent
Oxygen digestion generates negative impact.
Ligocellulose degradation leads table under 1 different condition of table
It is counted by the methane quantity obtained to embodiment 1- embodiment 13 and comparative example, obtains various pretreatments pair
The influence of gas production, Fig. 4 are the gas production of different pretreatments condition, from fig. 4, it can be seen that equal by pretreated each gas production
In 4000mL or more.And not pretreated gas production is 3318mL, it can thus be seen that pretreatment can greatly improve gas production.Wood
The influence of more pairs of gas production of matter cellulose degradation is not necessarily better, and excessive biogas slurry dosage easily causes to anaerobic digestion excessive
Burden, and handle the time more long lignocellulose element degradation reductions that will cause gas production more.Illustrate different pretreatment processing
Time is very big to the influence for producing depressed fruit.
It is each when obtaining gas production peak by the analysis of the above-mentioned pretreatment condition to embodiment 1- embodiment 13
The amount of single factor test: pretreatment biogas slurry additional amount is 20%TS, and pretreatment temperature is 30 DEG C, and pretreatment time is 6 days, according to center
Combination Design CCD principle, we set central point for the maximum point of above-mentioned each single factor test, pretreatment condition are rung
Face experimental design is answered, experimental factor level code table is obtained.Table 2 is experimental factor level code table.
2 empirical factor level code table of table
S2, testing program is determined using center combination design CCD principle
The experimental factor level code table obtained according to S1, selection pretreatment biogas slurry dosage, pretreatment temperature and pre- place
Reason three single factor tests of time are independent variable, using gas production as response, with center combination design CCD principle, carry out response surface
Experimental design；
S3, it completes to test according to testing program
According to the pretreatment for carrying out corn stover in the response surface experiments scheme of S2 design, pretreated corn stalk is used
Stalk natural pond, the biogas collected, and count；
Single factor experiment determine condition and range on the basis of, we respectively with A, B, C indicates pre-process biogas slurry additional amount,
Treatment temperature and pretreatment processing three independents variable of time are pre-processed, using gas production as response, with Design-Expert
8.06 center combination design CCD design principles therein, experimental factor level code table 2 are adjusted according to actual conditions, into
The design of row response surface experiments.The pretreatment that corn stover is carried out further according to the response surface experiments scheme of design, after pretreatment
Corn stover carry out natural pond processed, the biogas collected and statistics and the results are shown in Table 3 at testing program.As shown in Table 3, at 20 groups
For gas production between 3975mL~4853mL, gas production has 10 groups in the combination of 4500mL or more in experimental test data, wherein
Highest gas production is 4853mL.And untreated control group gas production is 2508mL.
3 response surface experimental design of table and result
S4, response surface model is established, analyzes test result
The test data input response surface software that S3 is obtained carries out regression analysis, is analyzed according to software as a result, obtaining single
The regression relation of factor and response obtains response surface three-dimensional figure and judges influence situation of each factor to response, finally
Horizontal parameters combination after determining each factors optimization.
Biogas output=+ 4766.02-82.44*A+24.27*B-91.68*C+74.00*A*B-16.00*A*C
+58.00*B*C-218.77*A2-67.79*B2-158.85*C2Formula 2
Formula 2 is the regression relation of obtained single factor test and response, wherein model coefficient of determination R2=0.9582, school
Positive R2=9206.Predict R2In the reasonable scope with signal-to-noise ratio.Table 4 is the analysis of variance table of model.It can be obtained from table 4
The F=25.48 of model out, corresponding P<0.0001 reach extremely significant level, and P=0.3503>0.05 for losing quasi- item is not significant,
Illustrate that the models fitting degree is preferable, test error is small, can be used for model analysis.
Influence size of each factor reflected from the F value of table 4 to gas production, can also be concluded that each examination
Test the primary and secondary sequence that factor influences gas production are as follows: C > A > B, i.e. time > biogas slurry dosage > temperature.
4 analysis of variance table of table
Fig. 5 is pretreatment biogas slurry additional amount and the contour that pretreatment temperature influences biogas output, and Fig. 6 is pretreatment natural pond
The response surface figure that liquid additional amount and pretreatment temperature influence biogas output, Fig. 7 are pretreatment temperature and pretreatment time to natural pond
The contour of gas yield effect, Fig. 8 is the response surface figure that pretreatment temperature and pretreatment time influence biogas output, from Fig. 5-
Fig. 8 can be seen that the significant interaction of pretreatment biogas slurry additional amount and temperature, and pretreatment biogas slurry additional amount is more, and (TS is got over
It is low), pretreatment temperature is higher or pretreatment biogas slurry additional amount fewer (TS is higher) and pretreatment temperature is lower that biogas can be obtained
Yield increases；With pretreatment biogas slurry additional amount reduction (raising of TS) and pretreatment temperature improve biogas yield not
It is disconnected to improve, but pretreatment biogas slurry additional amount is reduced when being increased to certain value with temperature to a certain extent, and the yield of biogas can generate
The phenomenon that reduction.The reciprocation and pretreatment biogas slurry additional amount and temperature of temperature and time and the friendship for pre-processing biogas slurry additional amount
Interaction result is consistent.
Each conspicuousness numerical value according to given by table 4 carries out the optimization of biogas slurry pretreatment parameter using the model in formula 2
Analysis, obtains the optimum condition of pretreating process.Namely optimize to obtain the pre- of corn stover anaerobism natural pond by response phase method
Processing optimised process is biogas slurry dosage 19.08%TS, 29.78 DEG C of temperature, is handled the time 5.42 days, theoretical gas production is
4786.3mL。
In order to verify the reliability of process conditions after optimization, we carry out natural pond processed using obtained pretreatment condition, and make
With unpretreated corn stover natural pond as a control group, pretreatment condition is set as biogas slurry dosage 19.08%TS, temperature 30
± 1 DEG C, processing the time be 5 days, under this process condition carry out 3 parallel tests, after measured ligocellulose degradation lead for
11.95 ± 0.58%, Fig. 9 are verification test gas production, and obtaining gas production from Fig. 9 is 4792 ± 48mL, are connect very much with predicted value
Closely.
Table 5 is the influence table of verification test group and control group to anaerobic digestion time, as can be seen from Table 5, verification test
The total gas production of group can be improved 30.76%, reaches the time used in 80% gas production and reduces 9 days, time shortening reaches
27.27%, and the production gas peak value of control group is only 255mL.Difference caused by intuitive performance gas production.
Influence of 5 different pretreatments of table to anaerobic digestion time
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of biogas slurry pre-processes corn stover natural pond method, which comprises the following steps:
Step 1, corn stalk raw material prepare
Corn stover is air-dried and crushed, is saved backup；
Step 2, biogas slurry preparation
Pretreatment biogas slurry: it is then uniformly mixed to gas is no longer produced within the biogas slurry standing sedimentation that batch-type dry fermentation is generated 3~7 days
It is 0.37% ± 0.11%, pH 7.2 to TS concentration, obtains pretreatment biogas slurry, it is spare；
Inoculation biogas slurry: the biogas slurry standing sedimentation 7~8 days in continuous stirred tank reactor is so incensed that be inoculated with biogas slurry to no longer producing,
The TS concentration of the inoculation biogas slurry is 4.36% ± 0.04%, pH 7.1；
Step 3, preprocess method
It weighs corn stover several pieces to be respectively placed in sealing container, be separately added into the pretreatment biogas slurry that step 2 obtains and mix,
So that the TS concentration of mixture is 10%~30%, pre-processed 2~10 days under 20~50 DEG C of constant temperatures after mixing evenly；
Step 4, anaerobic digestion
Corn stover pretreated in step 3 is uniformly mixed with the inoculation biogas slurry that step 2 obtains according to the mass ratio of 1:4, and
Being diluted with water to TS concentration is 8%, is sealed under 35 ± 1 DEG C of constant temperature and carries out anaerobic digestion.
2. a kind of biogas slurry according to claim 1 pre-processes corn stover natural pond method, which is characterized in that the step 1
3mm sieve is crossed after middle corn straw smashing.
3. a kind of biogas slurry according to claim 1 pre-processes corn stover natural pond method, which is characterized in that the step 3
The mass ratio of middle corn stover and pretreatment biogas slurry is 1:1.82~7.66.
4. a kind of biogas slurry according to claim 1 pre-processes corn stover natural pond method, which is characterized in that the step 4
The reactor of middle anaerobic digestion includes one be made of the graduated cylinder emulsion tube connection of the blue lid reagent bottle and a 1L of two 1L
Cover gas connection device, digestion bottle, biogas gas bottle of the blue lid reagent bottle of two 1L respectively as raw material, the graduated cylinder conduct of 1L
Catchment graduated cylinder.
5. a kind of condition of biogas slurry pretreatment corn stover natural pond method described in -4 any claims is excellent according to claim 1
Change method, which comprises the following steps:
S1, it determines single factor test, and transcoding, coding transform is carried out to it
The single factor test that biogas slurry dosage will be pre-processed, pretreatment temperature and pretreatment time optimize as pretreatment condition, then
Change single factor test respectively and carry out natural pond processed according to biogas slurry pretreatment corn stover natural pond method, the biogas collected simultaneously counts each list
The gas production of factor obtains the maximum each single factor test numerical value of gas production, establishes experimental factor level code table；
S2, testing program is determined using center combination design CCD principle
The experimental factor level code table obtained according to S1, when selection pre-processes biogas slurry dosage, pretreatment temperature and pretreatment
Between three single factor tests be independent variable, using gas production as response, with center combination design CCD principle, carry out response surface experiments
Design；
S3, it completes to test according to testing program
According to the pretreatment for carrying out corn stover in the response surface experiments scheme of S2 design, pretreated corn stover system is used
Natural pond, the biogas collected, and count；
S4, response surface model is established, analyzes test result
The test data input response surface software that S3 is obtained carries out regression analysis, is analyzed according to software as a result, obtaining single factor test
With the regression relation of response, obtains response surface three-dimensional figure and judge influence situation of each factor to response, it is final to determine
Horizontal parameters combination after each factors optimization.
6. a kind of condition optimizing method of biogas slurry pretreatment corn stover natural pond method according to claim 5, feature
It is, response surface software is 8.06 software of Design-Expert in S4.
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