CN109260377A - Has the orchid extract and combinations thereof of anti-fungus function - Google Patents

Has the orchid extract and combinations thereof of anti-fungus function Download PDF

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Publication number
CN109260377A
CN109260377A CN201710586576.4A CN201710586576A CN109260377A CN 109260377 A CN109260377 A CN 109260377A CN 201710586576 A CN201710586576 A CN 201710586576A CN 109260377 A CN109260377 A CN 109260377A
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orchid
extract
composition
seed
cymbidium seed
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蔡文杰
林彦志
许宗铭
萧郁芸
刘仲健
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Lin Shizhe
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Lin Shizhe
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Epidemiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Birds (AREA)
  • Cosmetics (AREA)

Abstract

A kind of orchid extract includes an at least anti-fungal composition, and extract extraction is from an orchid position, and the anti-fungal composition has antimycotic activity.A kind of orchid extract addition product is to make an addition to the orchid extract in one raw material, an antimycotic constituent is made.

Description

Has the orchid extract and combinations thereof of anti-fungus function
Technical field
A kind of orchid extract and combinations thereof of the present invention about antimycotic (anti-fungus) function of tool；Especially About a kind of cymbidium seed (orchid seed) for having anti-fungus function or quasi- protocorm body (protocorm) extract and its group Close object；More particularly about a kind of orchid extract of the albumen containing vicilin and combinations thereof for having anti-fungus function.
Background technique
Existing orchid extract, such as No. 201318647 " extraction of orchid extract of TaiWan, China patent application publication Method and its application " invention patent application case discloses a kind of orchid extract and its extracting process.The extracting process includes: Step 1, one orchid of extraction is carried out using water or methanol and obtain an orchid water extract or an orchid methanol extraction object；Step 2, Step 1 orchid methanol extraction object addition ethyl acetate obtained and water are extracted and obtain orchid ethyl acetate extraction Take object and an orchid water layer extract；And step 3, by the resulting orchid water layer extract of step 2 be added n-butanol and water into Row extracts and obtains an orchid n-butyl alcohol extract.
It holds, No. 201318647 orchid water extract, orchid methanol extraction object, orchid acetic acid ethyl ester extract Or orchid n-butyl alcohol extract has the activity for inhibiting Tyrosinase.In addition, the orchid water extract, orchid methanol extraction object, Orchid acetic acid ethyl ester extract or orchid n-butyl alcohol extract, which have, to be inhibited to draw with LPS (lipopolysaccharide) induction Inflammatory response effect of hair.
In fact, aforementioned disclose No. 201318647 only announcement orchid water extract, orchid methanol extraction object, orchid second Acetoacetic ester extract or orchid n-butyl alcohol extract have the activity and inflammatory response for inhibiting Tyrosinase.Therefore, existing Related orchid abstraction technique is there are still it is necessary to further provide for reaching other related efficacies using orchid extract, to carry out Extraction has the demand of the extract of antimycotic, skin care and antioxidant effect.
Another existing seed extract, such as TaiWan, China patent application publication the 201427674th " flower beans seed extraction Object and preparation method thereof and purposes " invention patent application case discloses a kind of colored beans (Phaseolus vulgarisL.) seed Extract and preparation method thereof.The extract preparation method includes: grinding the flower beans seed step a, to obtain a flower bean powder end； Step b, the flower bean powder end is dissolved using one first solvent to obtain homogeneous flower beans extract liquor；And step c, be centrifuged the homogeneous flower Beans extract liquor simultaneously collects blowing beans extract liquor on one.Step b, which is used, is about dissolved in first solvent with 1 g of the flower bean powder end 10 milliliters of ratio carries out.
It holds, which is selected from water, C1-C4 alcohols and its any combination.Or, first solvent is 50% ethanol water Solution (v/v), and the ethanol water is about 40 volume % to about 60 volume %.Or, first solvent is that 80% methanol is water-soluble Liquid (v/v), and the methanol aqueous solution is about 70 volume % to about 90 volume %.In fact, aforementioned disclose No. 201427674 only Disclose the relevant technologies of the flower beans seed extract and preparation method thereof with purposes.In fact, existing correlation seed extracts skill Art has antimycotic, skin care and anti-oxidant there are still it is necessary to further find the seed of other suitable substances, to carry out extraction The demand of the extract of effect.
The extract at another existing specific orchid position is " white such as TaiWan, China patent application publication the 201420107th Extract of gaily-colored butterfly orchid valve and preparation method thereof and purposes " invention patent application case, discloses a kind of white flower iris The extract of (Phalaenopsisamabilis or Phalaenopsis aphrodite) petal (petal) and its preparation side Method.The extract of the white flower iris petal can be used for promote skin-whitening, promoted skin moisture-retaining capacity and prevention and/ Or delay skin aging.In fact, it is aforementioned disclose No. 201420107 also only disclose white flower iris petal extract and its The relevant technologies of preparation method.Therefore, there are still it is necessary to further provide for utilizing it for existing related orchid abstraction technique Its orchid region of interest, to carry out the demand that extraction has the extract of antimycotic, skin care and antioxidant effect.
Another existing cosmetics of extract containing orchid, such as U.S. Patent Application Publication No. 20110002968 “Cosmetic orDermatological Composition Containing an Orchid Extract,and Cosmetic Care Method Using Said Composition " invention patent application case, discloses a kind of addition orchid The cosmetic composition and use cosmetic composition processing skin care method of extract.
Another existing cosmetics of extract containing orchid, such as U.S. Patent Application Publication No. 20090258093 " Use of a brassocattleya marcella koss orchid extract as an active agent to prevent Or delay the appearance of signs of cutaneous aging " invention patent application case, discloses one kind Using orchid extract as prevention or the activator of delay skin aging.
Another existing cosmetics of extract containing orchid, such as U.S. Patent Application Publication No. 20090238848 " Use of An Extract of the Orchid Vanda Coeruleaas a Skin Hydrating Agent " application for a patent for invention Case, disclose it is a kind of using great Hua all ages blue (Vanda coerulea) extract as skin moisturizer.
Another existing cosmetics of extract containing orchid, such as U.S. Patent Application Publication No. 20090232758 “Brassocattleya marcella koss orchid extract and use thereof as skin Depigmentation " invention patent application case discloses a kind of associated uses using orchid extract as skin stain removing.
In fact, aforesaid U.S. Patent application discloses No. 20110002968, No. 20090258093, No. 20090238848 and No. 20090232758 are all only disclosed and are handled using orchid extract as various skin cares, are not disclosed How using the extract of cymbidium seed antimycotic, skin care and oxidation resistant purpose reached.Therefore, existing orchid abstraction technique There are still it is necessary to further provide for using other orchid region of interest, to carry out the extraction of extraction tool skin care and antioxidant effect Take the demand of object.
Another existing cosmetics of extract containing orchid, such as No. 423826 " embryos containing orchid of TaiWan, China patent announcement I The cosmetics of element " patent of invention, discloses a kind of cosmetics of eggembryosin containing orchid.The toiletry bag is former containing an at least cosmetics Material and an at least orchid eggembryosin.The orchid eggembryosin is made an addition in the cosmetic material, a cosmetic composition is made. In addition, the orchid eggembryosin has the gene group of high performance, can produce to human skin beneficiating ingredient.
In fact, aforementioned TaiWan, China patent application publication, which announces I 423826, also only discloses the eggembryosin containing orchid The relevant technologies of cosmetics.Therefore, there are still it is necessary to further provide for using other for existing orchid embryo abstraction technique Orchid region of interest, to carry out the demand that extraction has the extract of antimycotic, skin care and antioxidant effect.Aforementioned all patent Shens Please case and patent be only the technology of the present invention background reference and the current technology state of development of explanation, not to limit this Invention.
In view of this, the present invention in order to meet the demand, provide a kind of orchid extract for having anti-fungus function and Its composition, wherein being made an addition in a raw material using an orchid extract, to form an antifungal composition, and the orchid extracts Object is taken to provide an anti-fungus function, and the orchid extract is the thick extract of an orchid, to expand answering for existing orchid extract Use range.
Summary of the invention
The main purpose of present pre-ferred embodiments is to provide a kind of orchid extract and combinations thereof for having anti-fungus function Object, wherein being made an addition in a raw material using an orchid extract, to form an antifungal composition, and the orchid extract is mentioned For an anti-fungus function, and the orchid extract is the thick extract of an orchid, provides antimycotic function to reach orchid extract Can, and reach the effect of natural (not toxic to human body) antifungal products are provided.
In order to achieve the above objectives, the orchid extract of present pre-ferred embodiments, it includes an extract, and the extraction Object is extracted from an orchid position, and the orchid extract includes an at least anti-fungal composition, and the anti-fungal composition is with anti-true The activity of bacterium.
The extract of present pre-ferred embodiments is a thick extract or a purifying extract.
The extract of present pre-ferred embodiments includes vicilin albumen and other effective components.
The extract of present pre-ferred embodiments has a predetermined concentration, and the predetermined concentration is 50ug/mL, 100ug/ ML, 150ug/mL, 200ug/mL or 400ug/mL.
The orchid position of present pre-ferred embodiments is a cymbidium seed, an orchid leaf, an orchid quasi- protocorm body, one Orchid portion, an orchid portion stamen column or other colored portion's organs.
The orchid extract addition composition of present pre-ferred embodiments includes:
An at least raw material have one first predetermined content；And
An at least orchid extract has one second predetermined content, and the orchid extract is extracted from an orchid position, The orchid extract is made an addition in the raw material, an antimycotic constituent is made；
Wherein the orchid extract includes an at least anti-fungal composition, and the anti-fungal composition has antimycotic activity.
The extract of present pre-ferred embodiments is a thick extract, and the extract or thick extract are suitable for inhibiting Fusarium, anthrax-bacilus, Sclerotiumrolfsii or other plant epiphytes.
The extract of present pre-ferred embodiments includes vicilin albumen and other effective components.
The extract of present pre-ferred embodiments has a predetermined concentration, and the predetermined concentration is 50ug/mL, 100ug/ ML, 150ug/mL, 200ug/mL or 400ug/mL.
The orchid position of present pre-ferred embodiments is a cymbidium seed, an orchid leaf, an orchid quasi- protocorm body, one Orchid portion, an orchid portion stamen column or other colored portion's organs.
The another object of present pre-ferred embodiments be the extract and its addition product of a kind of cymbidium seed are provided, wherein The extract of the cymbidium seed includes an at least lipid components, and the lipid components include an at least fatty acid, and the fatty acid It can be used as the application of anti-inflammatory, antibacterial and the physiological activity such as anti-oxidant, to reach the purpose for promoting cymbidium seed economic value.
In order to achieve the above objectives, the extract of the cymbidium seed of present pre-ferred embodiments includes an extract, extraction It is derived from a cymbidium seed, which includes an at least lipid components, and the lipid components include an at least fatty acid, and the rouge Fat acid can be used as the application of anti-inflammatory, antibacterial and the physiological activity such as anti-oxidant.
The cymbidium seed of present pre-ferred embodiments is selected from the cymbidium seed of iris.
The cymbidium seed of present pre-ferred embodiments is selected from the cymbidium seed of different growing stage.
The fatty acid of present pre-ferred embodiments includes palmitinic acid and linolenic acid.
The palmitinic acid of present pre-ferred embodiments and the content ratio of linolenic acid are 1:4.
In order to achieve the above objectives, the cymbidium seed extract addition product of present pre-ferred embodiments includes:
An at least cosmetic material has one first predetermined content；And
An at least cymbidium seed extract has one second predetermined content, and the cymbidium seed extract is extracted from one Cymbidium seed makes an addition to the cymbidium seed extract in the cosmetic material, a cosmetic composition is made；
Wherein the cymbidium seed extract includes an at least lipid components, and the lipid components include an at least fatty acid.
It is former that the cosmetic material of present pre-ferred embodiments is selected from a skin care products raw material, a color cosmetic raw material or a perfume Material, a skin care products, a color cosmetic or a perfume is made.
It is former that the cosmetic material of present pre-ferred embodiments is selected from a dusty raw materials, a paste raw materials or a liquid Material.
The cymbidium seed extract addition product of another preferred embodiment of the present invention includes:
An at least raw-food material has one first predetermined content；And
An at least cymbidium seed extract has one second predetermined content, and the cymbidium seed extract is extracted from one Cymbidium seed makes an addition to the cymbidium seed extract in the raw-food material, a food composition is made；
Wherein the cymbidium seed extract includes an at least lipid components, and the lipid components include an at least fatty acid.
The food composition of present pre-ferred embodiments is a beverage, a food, a sauce, a toppings, one strong Health food or a health treatment.
The cymbidium seed of present pre-ferred embodiments is selected from the cymbidium seed of iris.
The cymbidium seed of present pre-ferred embodiments is selected from the cymbidium seed of different growing stage.
The fatty acid of present pre-ferred embodiments includes palmitinic acid and linolenic acid.
The palmitinic acid of present pre-ferred embodiments and the content ratio of linolenic acid are 1:4.
Detailed description of the invention
Fig. 1 is the flow diagram of the extracting process of the cymbidium seed of the first preferred embodiment of the invention.
Figure 1A is the flow diagram that the orchid of the second preferred embodiment of the invention intends the extracting process of protocorm body.
Figure 1B is that the orchid extract of the tool anti-fungus function of present pre-ferred embodiments inhibits sickle born of the same parents to various concentration The schematic diagram of bacterium, anthrax-bacilus or Sclerotiumrolfsii experimental result.
Fig. 2 is the schematic diagram of the extract inner lipid component content of the cymbidium seed of present pre-ferred embodiments.
Fig. 3 is the schematic diagram of each fatty acid composition content in the extract of the cymbidium seed of present pre-ferred embodiments.
Fig. 4 is that the orchid extract of the first preferred embodiment of the invention makes an addition to the flow diagram of product operation.
Fig. 5 is that the extract of the cymbidium seed of the second preferred embodiment of the invention makes an addition to the process signal of product operation Figure.
Fig. 6 is that the extract of the cymbidium seed of third preferred embodiment of the present invention makes an addition to the process signal of product operation Figure.
Appended drawing reference
1: raw material；1a: cosmetic material；1b: raw-food material；2: orchid extract；20: cymbidium seed extract；3: Antimycotic constituent；3a: cosmetic composition；3b: food composition；100: process equipment.
Specific embodiment
In order to fully understand the present invention, elaborate in the attached drawing appended by preferred embodiment and cooperation that will hereafter illustrate, and It is not intended to limit the invention.
The orchid extract of the tool anti-fungus function of present pre-ferred embodiments, it adds product and its extracting process is applicable in In the extract of various orchids (such as: iris or other orchids) and its seed, its add product and its extracting process, but its The range being not intended to limit the invention.Furthermore the orchid extract of present pre-ferred embodiments makes an addition to various processed goods, example Such as: medical product, drug or makeups skin care products, but its range for being not intended to limit the invention.
For example, the orchid extract of the tool anti-fungus function of present pre-ferred embodiments selects an orchid position to carry out Extraction, and the orchid position be a cymbidium seed, an orchid leaf, an orchid intend protocorm body (or embryo portion), an orchid portion, One orchid portion stamen column or other colored portion's organs, but its range for being not intended to limit the invention.
The extract of the cymbidium seed of present pre-ferred embodiments is rich in grease (lipid) ingredient, and the lubricant component has There are anti-inflammatory ability, antibacterial ability and oxidation resistance.In addition, there is no specific peculiar smells for the extract of the cymbidium seed, and The shortcomings that will not influence food appearance (such as: food colors), therefore it is particularly suitable for making an addition to various beverage, food, seasoning Sauce, toppings, healthy food or health treatment.
For example, present pre-ferred embodiments selection uses the cymbidium seed of iris, and Phalaenopsis is in unifacial leaf Plant, and it has been found that need not have albuminosus tissue in the cymbidium seed of iris in academic research.Therefore, the present invention adopts It is proved in the cymbidium seed of iris really with the mode of histochemical stain rich in lubricant component.
Fig. 1 discloses the flow diagram of the extracting process of the cymbidium seed of the first preferred embodiment of the invention.Please refer to Fig. 1 Shown, the extracting process of the cymbidium seed of the first preferred embodiment of the invention includes step S1: firstly, providing the orchid of a predetermined amount Flower seed, and the cymbidium seed is subjected to pulverization process, to obtain a cymbidium seed fragment.For example, which selects From the cymbidium seed of iris, and the cymbidium seed is selected from the cymbidium seed of different growing stage.For example, it is crushed using one Device (blender, the stirrer) progress of machine (blender) or other tool agitating functions suitably fills a part crushing, stirring, to obtain The cymbidium seed fragment.
Referring again to shown in Fig. 1, the extracting process of the cymbidium seed of the first preferred embodiment of the invention includes step S2: being connect , selection in the container (container) by the cymbidium seed fragment make an addition to a solvent (such as: Hexane), to be formed One suspension (suspension).
Referring again to shown in Fig. 1, the extracting process of the cymbidium seed of the first preferred embodiment of the invention includes step S3: being connect , which is subjected to appropriate extraction processing, to obtain a cymbidium seed extract liquor.For example, which is extracted Take liquid that a cymbidium seed extract is made, and the cymbidium seed extract includes antimycotic (antifugal) ingredient, carbon hydrate Object (carbohydrate) ingredient, fatty acid (fatty acid) ingredient and other ingredients.
Referring again to shown in Fig. 1, the extracting process of the cymbidium seed of the first preferred embodiment of the invention includes step S4: most Afterwards, which is suitably analyzed into its ingredient with appropriate analysis instrument, with obtain an anti-fungal composition or its Its effective component.For example, which includes NMR spectroscopy analyzer (Nuclear Magnetic Resonance Spectroscopy, NMR), gas chromatography mass spectrometry analyzer (Gas Chromatography-Mass Spectrometry, GC-MS) or high-performance liquid chromatograph (High PerformanceLiquid Chromatography, HPLC)。
For example, the orchid kind of analysis iris is gone in present invention selection by the way of NMR spectroscopy (NMR) Contained ingredient in son, and it is rich in triglyceride in the cymbidium seed of iris as the result is shown.Meanwhile it then being analyzed not With the cymbidium seed of the iris of growth period, show with growth time, the fat content meeting of the cymbidium seed of iris As the time declines, i.e., it shows that the cymbidium seed of iris can provide these energy to sprout growth really.
The orchid that Figure 1A discloses the second preferred embodiment of the invention intends the flow diagram of the extracting process of protocorm body.It please join According to shown in Figure 1A, the extracting process that the orchid of the second preferred embodiment of the invention intends protocorm body includes step S1: firstly, providing one The orchid of predetermined amount intends protocorm body, and this is intended protocorm body and carries out pulverization process, to obtain a quasi- protocorm body fragment.Citing and Speech, which intends the quasi- protocorm body of orchid that protocorm body is selected from iris, and the orchid intends protocorm body and is selected from different growing stage Orchid intends protocorm body.For example, it carries out suitably filling part using the device (blender) of a pulverizer or other tool agitating functions Crushing, stirring, to obtain the quasi- protocorm body fragment.
Referring again to shown in Figure 1A, the extracting process that the orchid of the second preferred embodiment of the invention intends protocorm body includes step S2: then, selection in a container by this intend protocorm body fragment make an addition to a solvent (such as: Hexane), with formed one suspend Liquid.
Referring again to shown in Figure 1A, the extracting process that the orchid of the second preferred embodiment of the invention intends protocorm body includes step S3: then, which is subjected to appropriate extraction processing, to obtain a quasi- protocorm body extract liquor.For example, this is intended into protocorm Body extract liquor is made a quasi- protocorm body extract, and the quasi- protocorm body extract include anti-fungal composition, carbohydrate component, Fatty acid composition and other ingredients.
Referring again to shown in Figure 1A, the extracting process that the orchid of the second preferred embodiment of the invention intends protocorm body includes step S4: finally, this, which is intended protocorm body extract liquor, is suitably analyzed its ingredient with appropriate analysis instrument, to obtain an anti-fungal composition Or other effective components.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: preparation one First extraction buffer (extract buffer solution), it includes Tri(Hydroxymethyl) Amino Methane Hydrochloride (Tris- HCl) buffer (pH8.3,4.8g), lithium dodecyl sulfate (Lithium Dodecyl Sulfate) buffer (2.0g), sweet Oily (glycerol) buffer (35mL), ethylenediamine tetra-acetic acid (EDTA) buffer (0.5M stock, 0.4mL) and its mixing. In addition, secondary water (ddH is added in the extraction buffer2O) to 100mL (pH8.25-8.75), and -20 DEG C are stored in.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: another preparation One second extraction buffer (50mL), it includes the first extraction buffer (12.5mL), glycerol (glycerol) buffer (0.625mL), lithium dodecyl sulfate (Lithium Dodecyl Sulfate) buffer (0.75g), secondary water (ddH2O, 36.875mL) and its mixing.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: another preparation One protease inhibitors (protease inhibitor), it includes 1mM phenylmethylsulfonyl fluoride (PMSF, Phenymethylsulfonyl fluoride), 100mM phenylmethylsulfonyl fluoride (0.174g) methanol (MeOH, 1mL), the is added 1mM phenylmethylsulfonyl fluoride (0.1mL) is added in two extraction buffer (every 10mL) 1X Extraction Buffer.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: by an orchid Liquid nitrogen is added in flower position, and clays into power in a container (such as: mortar), to obtain an orchid powder.Then, by this Another container of orchid powder immigration (such as: 30mL glass tube) in, and the extraction of 2mL is added in the every 1 g orchid powder Buffer, to obtain the thick extract liquor of an orchid.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: by the orchid Thick extract liquor is spent to carry out one predetermined centrifugation time of centrifugation in a centrifuge with a desired speed and a predetermined temperature (such as: 4 DEG C) (such as: 3 minutes), centrifugate is slightly extracted to obtain an orchid.
For example, the extracting process of the antimycotic orchid extract of another preferred embodiment of the present invention includes: will collect The orchid slightly extracts the supernatant (containing protein) of centrifugate, and the supernatant contains the protein extraction object of anti-fungus function, And preferably collect the supernatant into a microcentrifugal tube (eppendorftube), with protein quantitative methods (BCA Method its concentration) is measured.
The orchid extract that Figure 1B discloses the tool anti-fungus function of present pre-ferred embodiments inhibits sickle to various concentration Born of the same parents bacterium (Fusarium oxysporum fsp.lycopersici), anthrax-bacilus (Colletotrichum capsici) or white thin,tough silk The schematic diagram of bacterium (Sclerotium rolfsii) experimental result (three experiments).It please refers to shown in Figure 1B, for example, this hair The various concentration of bright preferred embodiment selection use (such as: 50ug/mL, 100ug/mL, 150ug/mL, 200ug/mL, 400ug/ ML) effectively inhibit fusarium (as shown in the top of Figure 1B), anthrax-bacilus (as shown in the centre of Figure 1B), Sclerotiumrolfsii (such as Figure 1B Shown in bottom) or other plant epiphytes.
Referring again to shown in Figure 1B, the extract of present pre-ferred embodiments is a thick extract or a purifying extraction Object, and the extract includes vicilin albumen and other effective components, and the extract has a predetermined concentration, and this is predetermined Concentration is 50ug/mL, 100ug/mL, 150ug/mL, 200ug/mL, 400ug/mL.In addition, the orchid of present pre-ferred embodiments Flower position is a cymbidium seed, the quasi- protocorm body of an orchid, an orchid portion stamen column or other colored portion's organs.
Fig. 2 discloses the schematic diagram of the extract inner lipid component content of the cymbidium seed of present pre-ferred embodiments.It please join According to shown in Fig. 2, the triglyceride (triacylglycerol) that cymbidium seed includes is hydrolyzed, and with gas chromatography matter Spectrum analysis instrument (GC-MS) is analyzed.Then, compound molecule data bank, present invention hair are compared according to different delay times Lipid components are now rich in the cymbidium seed, and the lipid components include an at least fatty acid, and the fatty acid can be used as anti-hair The application of scorching, antibacterial and the physiological activity such as anti-oxidant.
Fig. 3 discloses the schematic diagram of each fatty acid composition content in the extract of the cymbidium seed of present pre-ferred embodiments, It is in comparison with Fig. 2.Referring to figure 2. and shown in Fig. 3, which mainly includes palmitinic acid (palmitic acid), linolenic acid (linolenic acid) and other fatty acid compositions.In addition, the palmitinic acid and linolenic acid of present pre-ferred embodiments Content ratio is 1:4.
Referring again to shown in Fig. 2 and Fig. 3, for example, palmitinic acid has effects that oxidation resistant；And the structure of linolenic acid Then has the unsaturated fatty acid of the ω -6 there are two double bond, therefore it has effects that anti-inflammatory, as shown in the right side of Fig. 3.
The orchid extract that Fig. 4 discloses the first preferred embodiment of the invention makes an addition to the flow diagram of product operation.Please Referring to shown in Fig. 4, the first preferred embodiment of the invention is by a raw material 1 and at least one or several orchid extracts (or orchid Extract liquor or powder) 2 an antimycotic constituent 3 is made in a process equipment 100 in appropriate proportions.
For example, the raw material 1 be an ointment raw material or a grease type, it includes vaseline (white or yellow), lanolin, It is menthol (oil), borneol, Borneolum, salicylic acid, wintergreen, camphor powder (oil), citronella oil, caryophyllus oil, silicone oil, butter, white Cured oil, linseed oil, oleic acid, turpentine oil, rosin, eucalyptus oil (You Jiali oil) or other.
The extract that Fig. 5 discloses the cymbidium seed of the second preferred embodiment of the invention makes an addition to the process signal of product operation Figure.Shown in referring to figure 5., relative to first embodiment, the second preferred embodiment of the invention is by a cosmetic material 1a and at least One or several kinds of cymbidium seed extracts (or cymbidium seed extract liquor or powder) 20 are made in the process equipment 100 in appropriate proportions At a cosmetic composition 3a.For example, it is fragrant to be selected from a skin care products raw material, a color cosmetic raw material or one by cosmetic material 1a Water raw material, a skin care products, a makeups skin care products, a color cosmetic or a perfume is made.In addition, cosmetic material 1a is selected from one Dusty raw materials, a paste raw materials or a liquid material.The makeups skin care products include skin conditioning article (such as: facial mask, creams Or Essence).
The extract that Fig. 6 discloses the cymbidium seed of third preferred embodiment of the present invention makes an addition to the process signal of product operation Figure.It please refers to shown in Fig. 6, relative to first embodiment, third preferred embodiment of the present invention is by a raw-food material 1b and at least one Kind or several cymbidium seed extracts (or cymbidium seed extract liquor or powder) 20 are made in the process equipment 100 in appropriate proportions One food composition 3b.For example, food composition 3b is selected as a beverage, a sauce, a toppings, a health food Product or a health treatment.Another preferred embodiment of the present invention will at least one or several kinds of cymbidium seed extract (or cymbidium seed Extract liquor or powder) 20 various processed goods are made an addition to, it includes a medical product, a drug or a makeups skin care products.
Aforementioned preferred embodiment only illustrates the present invention and its technical characteristic, and the technology of the embodiment still can be carried out suitably Various essence equivalent modifications and/or alternative are practiced；Therefore, interest field of the invention must regard the appended claims Subject to the range defined.

Claims (10)

1. a kind of orchid extract, which is characterized in that it include an extract, and the extract is extracted from an orchid position, and should Orchid extract includes an at least anti-fungal composition, and the anti-fungal composition has antimycotic activity.
2. orchid extract as described in claim 1, which is characterized in that the extract is a thick extract or a purifying extraction Object.
3. orchid extract as described in claim 1, which is characterized in that the extract includes vicilin albumen and at least one Effective component.
4. orchid extract as described in claim 1, which is characterized in that the extract has a predetermined concentration, and this is predetermined Concentration is 50ug/mL, 100ug/mL, 150ug/mL, 200ug/mL or 400ug/mL.
5. orchid extract as described in claim 1, which is characterized in that the orchid position is a cymbidium seed, an orchid leaf Son, an orchid intend protocorm body, an orchid portion, an orchid portion stamen column or a flower portion organ.
6. a kind of orchid extract addition composition, characterized by comprising:
An at least raw material have one first predetermined content；And
An at least orchid extract has one second predetermined content, and the orchid extract is extracted from an orchid position, by this Orchid extract makes an addition in the raw material, an antimycotic constituent is made；
Wherein the orchid extract includes an at least anti-fungal composition, and the anti-fungal composition has antimycotic activity.
7. orchid extract as claimed in claim 6 adds composition, which is characterized in that the extract is a thick extract, And the extract or thick extract are suitable for inhibiting fusarium, anthrax-bacilus, Sclerotiumrolfsii or other plant epiphytes.
8. orchid extract as claimed in claim 6 adds composition, which is characterized in that the extract includes vicilin egg A bletilla at least effective component.
9. orchid extract as claimed in claim 6 adds composition, which is characterized in that the extract makes a reservation for dense with one Degree, and the predetermined concentration is 50ug/mL, 100ug/mL, 150ug/mL, 200ug/mL, 400ug/mL.
10. orchid extract as claimed in claim 6 adds composition, which is characterized in that the orchid position is an orchid kind Son, an orchid leaf, an orchid intend protocorm body, an orchid portion, an orchid portion stamen column or a flower portion organ.
CN201710586576.4A 2017-07-18 2017-07-18 Has the orchid extract and combinations thereof of anti-fungus function Pending CN109260377A (en)

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CN110151914A (en) * 2019-04-09 2019-08-23 嘉兴市爵拓科技有限公司 Has the orchid extract and combinations thereof of anti-fungus function
WO2021196079A1 (en) * 2020-04-01 2021-10-07 梁家华 Energy-activating, anti-wrinkle, anti-inflammatory, and whitening orchid embryo tissue extract

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Publication number Priority date Publication date Assignee Title
CN110151914A (en) * 2019-04-09 2019-08-23 嘉兴市爵拓科技有限公司 Has the orchid extract and combinations thereof of anti-fungus function
WO2021196079A1 (en) * 2020-04-01 2021-10-07 梁家华 Energy-activating, anti-wrinkle, anti-inflammatory, and whitening orchid embryo tissue extract

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