CN109258815A - A kind of compound probiotic lactase modulation milk powder - Google Patents
A kind of compound probiotic lactase modulation milk powder Download PDFInfo
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- CN109258815A CN109258815A CN201811039925.1A CN201811039925A CN109258815A CN 109258815 A CN109258815 A CN 109258815A CN 201811039925 A CN201811039925 A CN 201811039925A CN 109258815 A CN109258815 A CN 109258815A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
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Abstract
The invention belongs to food technology fields, and in particular to a kind of compound probiotic lactase modulation milk powder.The present invention is mixed compound probiotic, lactase, prebiotics, milk powder, silica, protide raw material, minerals and sweetener by constant weight percentage, both solved the problems, such as that lactose intolerance crowd can not absorb lactose, intestinal flora is adjusted again, promotes intestinal absorption；The prebiotics and silica that the present invention adds can make the stability enhancing for modulating milk powder, keep and improve the activity of probiotics and lactase.
Description
Technical field
The invention belongs to food technology fields, and in particular to a kind of compound probiotic lactase modulation milk powder.
Background technique
Lactose is primarily present in milk and its product, needs to absorb by lactase breaks down.Alactasia meeting
Cause lactose digestion malabsorption, some patientss will appear abdominal distension, abdominal pain, a series of clinical symptoms based on diarrhea, referred to as newborn
Sugar does not tolerate.Alactasia accounts for 15% in Nordics, and Black people and people from Latin America account for 80%, and American Indians and Asian
Up to 100%.Some researches show that, in 3-5 years old, the 7-8 years old and 11-13 years old group children in China, alactasia and lactose intolerance
The incidence received is respectively 38.5%, 87.0%, 87.0% and 12.2%, 32.2%, 29.0%.Lactose intolerance not only influences
, there are malaise symptoms in the absorption of lactose in milk and its product, and protein, calcium, iron etc. in milk and other foods can also sought
That supports element is absorbed and utilized reduction, causes malnutrition.
Probiotics is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can generate
Definite health efficacy so as to improve host's microecological balance, play the active beneficial microorganism general name of beneficial effect.Human body moves
Beneficial bacterium or fungi mainly have in object: clostridium butyricum, lactobacillus, Bifidobacterium, actinomyces, saccharomycete etc..Generation at present
The most powerful product of the function of being studied in boundary be mainly the above all kinds of microorganism groups at composite reactive probiotics, be widely applied
In bioengineering, industrial or agricultural, food safety and life and health field.The file that planning commission issues over the years is defended according to country, is arrived
So far, the strain one that can be used for infant food shares 7, and the maximum probiotics supplier in the whole world has two, is respectively
U.S.'s Danisco and section of Denmark Hansen.The representative strain that the former supplies is Bi-07, NCFM and HOWARV series probiotics
(HN001,HN019)；The representative strain of the latter's supply is Bb-12 and LGG.
Lactose excessive in human body can be resolved into glucose and galactolipin by lactase.Glucose is partes corporis humani point metabolism
Energy source, it is necessity of infant brain development that galactolipin, which is then required structure sugar when National People's Congress's brain and mucous membrane tissue are metabolized,
Tissue, has close ties with shooting up for infant brain.Furthermore lactase can also be generated in human body by transglucosidation
Oligosaccharide, these oligosaccharide are a kind of low molecular weights, and not sticky water-soluble dietary fiber, it is used as proliferation factor in enteron aisle
It is only utilized by Bifidobacterium, is utilized without by putrefactivebacteria, can so greatly reduce the generation of harmful intestinal tract toxin, to prevention
Constipation and diarrhea play a very important role.Industrially lactase is produced using microbe fermentation method.The cream in different microorganisms source
The pH nature difference of carbohydrase is larger, is generally divided into neutral lactase and acid lactase.It is produced by Escherichia coli (E.Coli)
Lactase pH is 7.0~7.5；It is 6.0~7.0 by the saccharomycete person of being made；It is 5.0 or so by the mould person of being made.It is mended by external source
The case where filling the mode of lactase, can effectively solving lactose intolerance when intake dairy produce.
Patent document CN201410400017.6 discloses a kind of probiotics baby milk powder, the probiotics baby milk powder
Include lactose, vegetable oil, desalted whey powder, skimmed milk power, concentrated whey protein powder, complex prebiotics, mineral substance premix, flower
Raw tetraenoic acid Ultramicro-powder, docosahexaenoic acid Ultramicro-powder, vitamin premix, compound probiotic freeze-dried powder, lactoferrin, L-
The ingredients such as carnitine, taurine part and nucleotide, the milk powder can adjust infant's intestinal flora balance, promote enteron aisle it is unobstructed, suppression
Diarrhea processed and the immunity for improving infant, but wherein main component is lactose, is unable to satisfy the infant of lactose intolerance
Group.
Separately there is patent document CN201610170068.3 to disclose a kind of lactose-free baby formula milk powder and its preparation side
Method, the lactose-free baby formula milk powder contain vegetable fatty powder, Evamilk albumen and sepg whey albumen, although should
Lactose-free formula section directly avoids the generation of lactose intolerance in composition, but lactose be nutrition important in baby's growth and development at
Point, long-term lacking is also unfavorable for the growth and development of baby, the problem of can not fundamentally changing lactose intolerance.
We have found that probiotic supplemented can help to improve intestinal health, more conducively baby couple supplementing lactase simultaneously
Nutritional ingredient is absorbed and utilized, and achievees the effect that 1+1 is greater than 2, but product currently on the market or be a probiotic supplemented
And it is free of lactase or is only to add single probiotics or added probiotics is to make in infant food
, and also supported without enough science on additive amount.Furthermore lactase and probiotics are all active materials, steady
It is qualitative especially important, need formula, in terms of its stability is adequately investigated.
Summary of the invention
Compound probiotic lactase of the present invention modulates milk powder, is developed for existing problem, to be no less than 2
The probiotic powder that can be used for infant food of kind, compounding acid lactase is primary raw material, and adds prebiotics, milk powder, two
The auxiliary materials such as silica, protide raw material, minerals, sweetener are modulated milk powder made of the processes such as dressing sieve, total mix, packaging, tool
Play the role of solving lactose intolerance, adjust intestinal health, while there is high stable, mobility, the spy that dissolution is fast, mouthfeel is excellent
Point.
The present invention provides a kind of compound probiotic lactases to modulate milk powder, mainly by following components in percentage by weight group
At: compound probiotic 1%-5%, lactase 2%-6%, prebiotics 1%-10%, milk powder 60%-92%, silica
0.1%-1%, protide raw material 0%-8%, minerals 0-10% and sweetener 0%-10%.
Preferably, compound probiotic lactase modulation milk powder consists of the following components in percentage by weight: main
It consists of the following components in percentage by weight: compound probiotic 1%, lactase 5%, prebiotics 4%, milk powder 89% and titanium dioxide
Silicon 1%.
Preferably, the compound probiotic is animal bifidobacteria, bifidobacterium lactis, Lactobacillus rhamnosus, Luo Yishi
Two or three or more in lactobacillus, lactobacillus fermenti and bifidobacterium breve.
Preferably, the compound probiotic is that bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio are formed.
Preferably, the silica the preparation method comprises the following steps:
By the ammonium hydroxide of ethyl orthosilicate and 1mol/L instill 30% (v/v) ethyl alcohol in, the ethyl orthosilicate, ammonium hydroxide and
The volume ratio of ethyl alcohol is 2:1:8, stirs 30min, stands, precipitates, washs, is drying to obtain.
Preferably, the silica partial size is 30-80nm, specific surface area 100-360m2/g。
Preferably, the lactase is acid lactase；The milk powder is lactinated skimmed milk power or whole milk
Powder.
Preferably, the prebiotics are one in oligofructose, galactooligosaccharide, inulin, stachyose and dietary fiber
Kind is several.
Preferably, the protide raw material is one of animal protein and vegetable protein or combinations thereof.
Preferably, the minerals are the one or more of magnesium chloride, calcium gluconate and zinc gluconate；Described
Sweetener is the one or more of mogroside, xylitol, steviol glycoside and Sucralose.
The silica added in compound probiotic lactase modulation milk powder provided by the invention is as anticaking agent, defoaming
Agent can be such that lactase and milk powder, protein matter etc. is uniformly mixed, meanwhile, the partial size of the homemade silica of the present invention is 30-
80nm, specific surface area is in 240-400m2Between/g, it can be risen in compound probiotic lactase modulation milk powder and alleviate milk powder moisture absorption
The phenomenon that agglomeration, can also maintain the activity of probiotics and lactase, extend effective holding time of probiotics and lactase.
Compound probiotic provided by the invention, can be improved the assimilation effect of enteron aisle, and improve probiotics and lactase
Activity.
Compared with prior art, compound probiotic lactase provided by the invention modulation milk powder have it is following the utility model has the advantages that
1. compound probiotic and acid lactase are combined by the present invention, not only solves lactose intolerance issue, but also can
To adjust intestinal health, promote intestinal absorption, milk and other foods is cooperated to be used together, improves what nutrient was absorbed and used
Effect.
2. prebiotics and silica that the present invention adds can make the stability enhancing for modulating milk powder, keeps and improve and is prebiotic
The activity of bacterium and lactase.
Specific embodiment
It is as described below, it is merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.The present invention is using ingredient
Conventional commercially available composition, wherein bifidobacterium lactis HN019, lot number 1103159361, Lactobacillus rhamnosus HN001, lot number
11031703, producer: Danisco；Animal bifidobacteria BB-12, lot number 3389371, producer: section's Hansen；Acid lactase by
Aspergillus niger and aspergillus oryzae ferment and obtain；Orange powder: opening up rich powder technology (Kunshan) Co., Ltd, model SD1001, and lot number is
2018051701；Calcium gluconate: Zhejiang Tianyi Food Additives Co., Ltd., lot number 20180408；Xylitol: Shandong dragon power
Biotech inc, lot number 18060201, mogroside: Hunan Nutramax Inc., type
Number 25% glycosides V, lot number OGLHG-180305-05.
The preparation of embodiment 1, a kind of compound probiotic lactase modulation milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, galactooligosaccharide 4%, lactose whole milk powder 89%, silica 1 %.
The compound probiotic is that bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio are formed；
The silica partial size is 30-80nm, specific surface area 100-360m2/ g, the preparation method comprises the following steps:
By the ammonium hydroxide of ethyl orthosilicate and 1mol/L instill 30% (v/v) ethyl alcohol in, the ethyl orthosilicate, ammonium hydroxide and
The volume ratio of ethyl alcohol is 2:1:8, stirs 30min, stands, precipitates, washs, is drying to obtain.
Compound probiotic lactase modulation milk powder the preparation method comprises the following steps:
I, by galactooligosaccharide, skimmed milk power, silica sieves with 100 mesh sieve respectively, uniformly mixed, obtains mixture A；
II, in the case where temperature is 15-25 DEG C of environment, compound probiotic and acid lactase are added obtained in step I and mixed
Close object A in, be put into screw mixer and mix, vacuum be filled with inert gas pouch-packaged to get.
Embodiment 2, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, oligofructose 2%, galactooligosaccharide 2%, whole milk powder containing lactose 89%, silica 1 %.
The compound probiotic is made of bifidobacterium lactis and Lactobacillus rhamnosus 1:1 in mass ratio.
The silica is the compound probiotic lactase modulation milk powder preparation in embodiment 1 obtained by preparation method
Method is similar to Example 1.
Embodiment 3, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulation milk powder consists of the following components in percentage by weight: compound probiotic 5%,
Acid lactase 6%, oligofructose 2%, galactooligosaccharide 2%, lactose whole milk powder 84%, silica 1 %.
The compound probiotic is animal bifidobacteria, bifidobacterium lactis and Lactobacillus rhamnosus 1:1:1 group in mass ratio
At.
The silica is the compound probiotic lactase modulation milk powder preparation in embodiment 1 obtained by preparation method
Method is similar to Example 1.
Comparative example 1, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, galactooligosaccharide 4%, lactose whole milk powder 91%, silica 1 %.
The silica is the compound probiotic lactase modulation milk powder preparation in embodiment 1 obtained by preparation method
Method is similar to Example 1.
The difference from embodiment 1 is that using single probiotics cream Bifidobacterium Bifidum.
Comparative example 2, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, lactose whole milk powder 93%, silica 1 %.
The compound probiotic is that bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio are formed.
The silica is the compound probiotic lactase modulation milk powder preparation in embodiment 1 obtained by preparation method
Method is similar to Example 1.
The difference from embodiment 1 is that not adding prebiotics.
Comparative example 3, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, galactooligosaccharide 4%, lactose whole milk powder 89%, magnesium carbonate 1%.
The compound probiotic is that bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio are formed.
The compound probiotic lactase modulation method for preparing milk is similar to Example 1.
The difference from embodiment 1 is that replacing silica with magnesium carbonate.
Comparative example 4, a kind of compound probiotic lactase modulate milk powder
The compound probiotic lactase modulates milk powder, consists of the following components in percentage by weight: compound probiotic
1%, acid lactase 5%, galactooligosaccharide 4%, lactose whole milk powder 89%, silica 1 %.
The compound probiotic is that bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio are formed.
The compound probiotic lactase modulation method for preparing milk is similar to Example 1.
The difference from embodiment 1 is that it is 100-300nm, specific surface area 20-60m that silica, which is commercially available partial size,2/
The silica of g.
Test example 1, probiotic active detection
1. subjects: the compound probiotic lactase of embodiment 1-3 and comparative example 1-4 preparation modulates milk powder
2. test method:
Measurement embodiment 1-3 is with comparative example 1-4 compound probiotic lactase modulation milk powder in 0d, 7d, 14d, 21d respectively
Number of live bacteria of probiotics afterwards, preservation condition: 10-25 DEG C of sealing is kept in dark place.
3. test result:
Test result such as table 1.
1 number of live bacteria of probiotics of table (CFU/g)
| Group | 0d | 7d | 14d | 21d |
| Embodiment 1 | 7.8×109 | 6.7×109 | 6.7×109 | 4.7×109 |
| Embodiment 2 | 7.0×109 | 6.5×109 | 5.0×109 | 3.9×109 |
| Embodiment 3 | 7.2×109 | 7.5×109 | 6.8×109 | 6.7×108 |
| Comparative example 1 | 3.4×109 | 6.0×108 | 6.1×107 | 1.0×106 |
| Comparative example 2 | 5.0×109 | 4.4×108 | 3.6×107 | 8.0×105 |
| Comparative example 3 | 6.4×109 | 4.5×106 | 8.4×105 | 4.4×104 |
| Comparative example 4 | 6.7×109 | 4.0×106 | 9.8×105 | 8.8×104 |
As shown in Table 1:
(1) embodiment of the present invention 1, embodiment 2 and embodiment 3 are in 21d, and probiotics survival rate is high, and in its effective model
Enclose 1 × 107-1×1010In CFU/g, it is embodiment 1 that wherein probiotics survival rate is highest, compound in conjunction with the explanation of comparative example 1
It modulates and contains prebiotic strain of more than two kinds in milk powder, probiotics survival rate can be improved；
(2) such as comparative example 3 and 4, the partial size of silica or silica is not added not within the scope of 30-80nm, benefit
The extension at any time of the survival number of raw bacterium and reduce, and be unable to reach the quantitative range of edible addition, illustrate to make using the present invention
Silica of the partial size obtained within the scope of 30-80nm, can be improved probiotics survival rate；
(3) comparative example 2 illustrates, the stability of probiotics can be improved in addition galactooligosaccharide.
Test example 2, lactase activity detection
1. subjects: the compound probiotic lactase of embodiment 1-3 and comparative example 1-4 preparation modulates milk powder.
2. test method:
The lactose enzyme activity of the compound probiotic lactase modulation milk powder of embodiment 1-3 and comparative example 1-4 preparation is measured respectively
Property, 0-4 DEG C of preservation, respectively in the lactase activity of 0d, 7d, 14d and 21d measurement compound probiotic lactase modulation milk powder.
3. test result:
Test result such as table 2
2 lactase activity of table (ALU)
As shown in Table 2:
(1) Example 1 and Example 2 of the present invention is in 21d, though lactase activity is declined, still exists after 21 days
Effective range, and activity is 4 times or more of single probiotics in comparative example 1；
(2) comparative example 3 and 4 does not add the partial size of silica and silica not within the scope of 30-80nm, lactose
The active of enzyme sharply declines in 21d, and beyond edible effective active range 2000-10000ALU；
(3) comparative example 2 illustrates, adding oligofructose and galactooligosaccharide can make the stability of lactase improve 2.5 times of left sides
It is right.
Claims (10)
1. a kind of compound probiotic lactase modulates milk powder, which is characterized in that mainly consist of the following components in percentage by weight:
Compound probiotic 1%-5%, lactase 2%-6%, prebiotics 1%-10%, milk powder 60%-92%, silica 0.1%-
1%, protide raw material 0%-8%, minerals 0-10% and sweetener 0%-10%.
2. compound probiotic lactase according to claim 1 modulates milk powder, mainly by following components in percentage by weight
Composition: compound probiotic 1%, lactase 5%, prebiotics 4%, milk powder 89% and silica 1 %.
3. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the compound benefit
Raw bacterium is animal bifidobacteria, bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus fermenti and short bifid bar
Two or three or more in bacterium.
4. compound probiotic lactase according to claim 3 modulates milk powder, which is characterized in that the compound probiotic
For bifidobacterium lactis and Lactobacillus rhamnosus 3:2 in mass ratio composition.
5. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the titanium dioxide
Silicon the preparation method comprises the following steps:
The ammonium hydroxide of ethyl orthosilicate and 1mol/L are instilled in the ethyl alcohol of 30% (v/v), the ethyl orthosilicate, ammonium hydroxide and ethyl alcohol
Volume ratio be 2:1:8, stir 30min, stand, precipitate, wash, be drying to obtain.
6. compound probiotic lactase according to claim 5 modulates milk powder, which is characterized in that the titanium dioxide silicon grain
Diameter is 30-80nm, specific surface area 100-360m2/g。
7. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the lactase
For acid lactase；The milk powder is lactinated skimmed milk power or whole milk powder.
8. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the prebiotics
For one or more of oligofructose, galactooligosaccharide, inulin and stachyose.
9. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the protide
Raw material is one of animal protein and vegetable protein or combinations thereof.
10. compound probiotic lactase according to claim 1 or 2 modulates milk powder, which is characterized in that the minerals
For the one or more of magnesium chloride, calcium gluconate and zinc gluconate；The sweetener be mogroside, xylitol,
The one or more of steviol glycoside and Sucralose.
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| CN114209817A (en) * | 2021-12-30 | 2022-03-22 | 山东源科生物科技股份有限公司 | Production process of liposome-coated lactase |
| CN114617168A (en) * | 2022-03-30 | 2022-06-14 | 四川金汇源农业科技有限责任公司 | Milk powder formula suitable for lactose intolerant people and preparation method thereof |
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