CN109258695A - Application of the Pseudomonas chlororaphis in prevention and treatment nematode - Google Patents
Application of the Pseudomonas chlororaphis in prevention and treatment nematode Download PDFInfo
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- CN109258695A CN109258695A CN201811214677.XA CN201811214677A CN109258695A CN 109258695 A CN109258695 A CN 109258695A CN 201811214677 A CN201811214677 A CN 201811214677A CN 109258695 A CN109258695 A CN 109258695A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
Application of the Pseudomonas chlororaphis (Pseudomonas chlororaphis subsp.aureofaciens) in prevention and treatment nematode, the Pseudomonas chlororaphis are Pseudomonas chlororaphis GDMCC No.60273.Application of the Pseudomonas chlororaphis in the bacteria agent of preparation prevention and treatment nematode, the bacteria agent include the Pseudomonas chlororaphis and carrier as active constituent, and the bacteria agent is Pseudomonas chlororaphis fermentation liquid.The method for preventing and treating nematode, is sprayed on affected part for the bacteria agent containing Pseudomonas chlororaphis and/or its fermentation liquid in claim 1.The application of Pseudomonas chlororaphis of the invention in prevention and treatment nematode prevents and treats nematode, non agricultural chemical residuum with Pseudomonas chlororaphis, and non-environmental-pollution does not cause security threat to people and animals；With the bacteria agent of the fermentation liquid preparation prevention and treatment nematode of Pseudomonas chlororaphis, preparation process is simple, and at low cost, effect is good, easy to use.
Description
Technical field
The present invention relates to the application of biological control and bacteria agent more particularly to Pseudomonas chlororaphis in prevention and treatment nematode.
Background technique
The various plants such as pathogenic pest damage forest, grain, veterinary antibiotics, host range is wide, concealment is strong,
The features such as prevention and control are difficult, it has also become threaten agricultural, the key disease of forestry, cause extreme influence to national economy production.
The whole world for plant, crop disease and insect prevention and treatment still based on chemical prevention, although Chemical control methods insecticide efficiencies is high, its
Have the defects that high toxicity, easily residual, pollution environment etc..Attention with China to green agriculture, research and development green
The pathogenic nematode Control Technology of safety and environmental protection is to promote the inevitable behave of economy-ecological balance development.
Whole world research related with nematode in recent years is more, is related to from the every aspect for being categorized into prevention and treatment, in cause of disease, matchmaker
The numerous areas such as Jie, gene, behavior and fungal component but generally still wreak havoc Wuji without too quantum jump, nematodiasis.
The control method of nematodiasis mainly has physical control, chemical prevention and biological control.Physical control includes eliminating wash, is hot
Processing, ray and ultrasonication etc., applying in physical control method at most is heat treatment, hot water immersion treatment, benefit
Nematode is killed with high temperature.Hot-water soak method can reache a certain level the effect of prevention and treatment nematode, but since the water temperature of processing is not allowed
It is easy to control, easily cause dead seedling situation etc..Therefore, efficient, less toxic, the free of contamination biological prevention and control agent that exploitation is directed to nematode is found, is
Control, the most direct and most economical effective approach for administering nematodiasis.
Caenorhabditis elegans Caenorhabditis elegans is with growth demand is simple, life cycle is short, fecundity
The features such as power is strong and genetic background understands, it has also become the excellent mode of one of pathogenetic bacteria-host's interrelationship study is raw
Object.The intracorporal research of parasitic nematode at present has some limitations, so being compared research with Caenorhabditis elegans is
A kind of effective and reasonable method.Since the structure of parasitic nematode and Caenorhabditis elegans, development, reproduction etc. have many phases
Like place, the gene compared with detailed research and the homologous parasitic nematode of Caenorhabditis elegans can be used to by seeking free living system.
It is in recent years mode using C.elegans, people kill line bacterial strain and research pathogen in screening acquisition and host is mutual
Make mechanism etc. and achieves great successes.Criffitts et al. discloses bacillus thuringiensis Bacillus
The parasporal crystal of thuringiensis kills line mechanism (Griffitts et al., 2001&2005；Peng Qi etc., 2015)；Niu
Deng unique nematicidal new mechanism --- " Trojan Horse " mechanism for then having found that pathogenetic bacteria B.nematodida possesses
(Niu QH et al., 2010), Zhang etc. then find the cause of disease the mechanism of action (the Zhang CM et that bacterium attracts nematode to colonize
al.,2016)。
Therefore, using C.elegans as model organism, the anti-line resource of separation screening high-efficiency low-toxicity deeply understands cause of disease
Bacterium kills line mechanism, improves biocontrol microorganisms to the pathogenic of nematode, is the key that improve nematode biological prevention and control agent insecticide efficiency urgent need to resolve
Problem.
Summary of the invention
In order to overcome chemical pesticide environmental pollution present in prevention and treatment nematode, pesticide residue and threat human and livestock health etc. to ask
Topic, the present invention provides Pseudomonas chlororaphis (Pseudomonas chlororaphis subsp.aureofaciens) GDMCC
Application of the No.60273 in prevention and treatment nematode, this constitutes the first aspect of the invention.
The present invention also provides application of the Pseudomonas chlororaphis in the bacteria agent of preparation prevention and treatment nematode, including as work
Property ingredient Pseudomonas chlororaphis and carrier, this constitute the second aspect of the invention.
The present invention also provides a kind of methods for preventing and treating nematode, will contain Pseudomonas chlororaphis GDMCC No.60273's
And/or the bacteria agent of its fermentation liquid is sprayed on affected part, this constitutes the third aspect of the invention.
Beneficial effects of the present invention: nematode, non agricultural chemical residuum, non-environmental-pollution, not to people are prevented and treated with Pseudomonas chlororaphis
Poultry causes security threat；With the bacteria agent of the fermentation liquid preparation prevention and treatment nematode of Pseudomonas chlororaphis, preparation process is simple, cost
Low, effect is good, easy to use.
Pseudomonas chlororaphis (Pseudomonas chlororaphis subsp.aureofaciens) of the invention has existed
It is disclosed in the bacterial strain of Chinese invention patent CN201810082686 production escaping gas antagonism sweet potato black rot pathogen and its application,
Deposit number is GDMCC No.60273, and the culture presevation date is on November 8th, 2017, specific name are as follows: Pseudomonas chlororaphis
Pseudomonas aureofaciens subspecies (Pseudomonas chlororaphis subsp.aureofaciens) SPS-41, preservation list
The entitled Guangdong Province's Culture Collection in position, address are Guangdong institute of microbiology of GuangZhou, China city, postcode 510075.
Confirm through depositary institution, which is in existing state.
Detailed description of the invention
Fig. 1 is that the raw of the Pseudomonas chlororaphis eelworm-killing activity in the embodiment of the present invention surveys result.
Specific embodiment:
Embodiment
1 bacterial growth media LB
Peptone 10g/L, yeast extract 5g/L, NaCl 10g/L adjust pH7.0, autoclave sterilization 20min.Add 2%
Agar powder is up to LB solid medium.
2 kill line Activity determination culture medium
NGM culture medium: 3g sodium chloride is dissolved with 975ml distilled water, 17g agar, 2.5g polyprotein peptone, high steam goes out
Bacterium.After being cooled to 55 DEG C or so, 1ml 1mol/L CaCl is added2, 1ml 1mol/L MgS04, 1ml 5mg/L cholesterol,
25ml kaliumphosphate buffer.Culture dish is poured into after mixing.
Nematode Bleach lysate:
5M NaOH 0.3ml
Sodium hypochlorite 1.2ml
ddH2O 3.5ml
Phosphate buffer
KH2PO4 119g
K2HPO4 21.5g
ddH2O adds water to 1L
0.22um filtration sterilization.
3 nematode cryopreservation methods
Caenorhabditis elegans can freeze in -80 degree (Liang, J., et al., 2003), freeze the specific method of nematode
It is as follows:
1, nematode is cultivated on NGM plate；
2, when nematode culture to majority is hungry L1/L2 nematode, nematode is washed down with M9 buffer, is washed 2 times；
3, four cryopreservation tubes are taken, 0.5ml nematode suspension and 0.5ml frozen stock solution are added in each cryopreservation tube, after mixing,
It is saved in -80 refrigerators；Or it is added at one time 2ml nematode suspension and 2ml frozen stock solution, it is distributed into four pipes；
4, second day, one taken out in four cryopreservation tubes recovered, and the method for recovery is that cryopreservation tube is taken out room
Temperature is melted, and nematode suspension is added on the NGM plate containing OP50 after dissolving and is cultivated, and sees line inspection worm recovery situation.
4 nematode cultures and synchronization
Solid medium culture nematode (Lewis and Fleming, 1995；Brenner,1974)
1, OP50 is activated: the E.coli OP50 frozen is crossed on LB plate, 37 DEG C of overnight incubations；
2, it chooses single colonie and is inoculated into liquid LB culture, 37 DEG C of overnight incubations；
3,50-200 μ l OP50 bacterium solution is added dropwise in the center of 60mm NGM plate, and is dried up in grasping net platform；
4,37 DEG C of cultures will be placed in added with the plate of OP50；
5, nematode is washed down with M9 buffer, is added on the plate with OP50, cultivated in 20 DEG C.
5 bacterium eelworm-killing activities are raw to be surveyed
1, by Pseudomonas chlororaphis GDMCC No.60273 bacterial strain in 37 DEG C of overnight incubations of LB lining out；
2, it chooses single bacterium to fall in liquid LB test tube, 37 DEG C, 180rpm overnight incubation；
3,100 μ l bacterium solutions are taken to be coated on NGM (32 μM) plate, 37 DEG C of culture 12h, then at 25 DEG C of culture 12h；
4, the L4 nematode handled well is added to containing on germy NGM plate, is counted every 12h, statistical result is such as
Shown in Fig. 1, it can be seen that Pseudomonas chlororaphis plays good inhibiting effect to L4 nematode.
Claims (5)
1. Pseudomonas chlororaphis (Pseudomonas chlororaphis subsp.aureofaciens) is in prevention and treatment nematode
Using.
2. application according to claim 1, which is characterized in that the Pseudomonas chlororaphis is Pseudomonas chlororaphis
GDMCCNo.60273。
3. application of the Pseudomonas chlororaphis in the bacteria agent of preparation prevention and treatment nematode in claim 1, which is characterized in that institute
Stating bacteria agent includes the Pseudomonas chlororaphis and carrier as active constituent.
4. application of the Pseudomonas chlororaphis according to claim 3 in the bacteria agent of preparation prevention and treatment nematode, feature
It is, the bacteria agent is Pseudomonas chlororaphis fermentation liquid.
5. a kind of method for preventing and treating nematode, which is characterized in that the Pseudomonas chlororaphis and/or its hair in claim 1 will be contained
The bacteria agent of zymotic fluid is sprayed on affected part.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811214677.XA CN109258695A (en) | 2018-10-18 | 2018-10-18 | Application of the Pseudomonas chlororaphis in prevention and treatment nematode |
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148791A (en) * | 1994-04-18 | 1997-04-30 | 瑞典农场主全国经济协会 | Composition and method for controlling plant diseases |
| CN103109870A (en) * | 2013-01-08 | 2013-05-22 | 华中农业大学 | Application of fermentation supernatant liquid of pseudomonas |
| CN103602621A (en) * | 2013-12-02 | 2014-02-26 | 江苏省农业科学院 | Pseudomonas choloeaphtis and application |
| CN104017744A (en) * | 2013-11-07 | 2014-09-03 | 上海交通大学 | Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth |
| CN104031859A (en) * | 2014-05-13 | 2014-09-10 | 华中农业大学 | Pseudomonas syringae, screening method thereof and application to kill nematode |
| CN104968672A (en) * | 2012-07-02 | 2015-10-07 | 先锋国际良种公司 | Novel insecticidal proteins and methods for their use |
| CN105112339A (en) * | 2015-09-15 | 2015-12-02 | 沈阳农业大学 | Novel bacteria strain and culture method and application thereof |
| CN105219671A (en) * | 2015-09-15 | 2016-01-06 | 沈阳农业大学 | A kind of bacterial isolates and cultural method thereof and application |
| CN105950516A (en) * | 2016-06-30 | 2016-09-21 | 北京林业大学 | Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain |
| CN107099474A (en) * | 2017-05-12 | 2017-08-29 | 山东省烟台市农业科学研究院 | A kind of Pseudomonas chlororaphis and its application with broad spectrum antibiotic activity |
| CN108018232A (en) * | 2017-12-01 | 2018-05-11 | 华南农业大学 | A kind of radopholus similes thorne associated bacteria with genetic modification potentiality |
| CN108018305A (en) * | 2017-12-01 | 2018-05-11 | 华南农业大学 | A kind of radopholus similes thorne control method mediated using radopholus similes thorne associated bacteria |
| CN108148782A (en) * | 2018-01-29 | 2018-06-12 | 江苏师范大学 | Produce bacterial strain and its application of escaping gas antagonism sweet potato black rot pathogen |
-
2018
- 2018-10-18 CN CN201811214677.XA patent/CN109258695A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148791A (en) * | 1994-04-18 | 1997-04-30 | 瑞典农场主全国经济协会 | Composition and method for controlling plant diseases |
| CN104968672A (en) * | 2012-07-02 | 2015-10-07 | 先锋国际良种公司 | Novel insecticidal proteins and methods for their use |
| CN103109870A (en) * | 2013-01-08 | 2013-05-22 | 华中农业大学 | Application of fermentation supernatant liquid of pseudomonas |
| CN104017744A (en) * | 2013-11-07 | 2014-09-03 | 上海交通大学 | Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth |
| CN103602621A (en) * | 2013-12-02 | 2014-02-26 | 江苏省农业科学院 | Pseudomonas choloeaphtis and application |
| CN104031859A (en) * | 2014-05-13 | 2014-09-10 | 华中农业大学 | Pseudomonas syringae, screening method thereof and application to kill nematode |
| CN105112339A (en) * | 2015-09-15 | 2015-12-02 | 沈阳农业大学 | Novel bacteria strain and culture method and application thereof |
| CN105219671A (en) * | 2015-09-15 | 2016-01-06 | 沈阳农业大学 | A kind of bacterial isolates and cultural method thereof and application |
| CN105950516A (en) * | 2016-06-30 | 2016-09-21 | 北京林业大学 | Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain |
| CN107099474A (en) * | 2017-05-12 | 2017-08-29 | 山东省烟台市农业科学研究院 | A kind of Pseudomonas chlororaphis and its application with broad spectrum antibiotic activity |
| CN108018232A (en) * | 2017-12-01 | 2018-05-11 | 华南农业大学 | A kind of radopholus similes thorne associated bacteria with genetic modification potentiality |
| CN108018305A (en) * | 2017-12-01 | 2018-05-11 | 华南农业大学 | A kind of radopholus similes thorne control method mediated using radopholus similes thorne associated bacteria |
| CN108148782A (en) * | 2018-01-29 | 2018-06-12 | 江苏师范大学 | Produce bacterial strain and its application of escaping gas antagonism sweet potato black rot pathogen |
Non-Patent Citations (5)
| Title |
|---|
| A.V. STURZ1 ET AL: "Endoroot bacteria derived from marigolds (Tagetes spp.) can decrease soil population densities of root-lesion nematodes in the potato root zone", 《PLANT AND SOIL》 * |
| V. INSUNZA ET AL: "Root bacteria from nematicidal plants and their biocontrol potential against trichodorid nematodes in potato", 《PLANT AND SOIL》 * |
| 唐珊熙: "《微生物学及微生物学检验》", 31 May 1998, 人民卫生出版社 * |
| 范津: "丁香假单胞菌MB03及铜绿假单胞菌中对线虫毒性蛋白筛选与鉴定", 《中国优秀说书学位论文全文数据库 农业科技辑》 * |
| 黄金玲等: "植物寄生线虫生防细菌的研究进展", 《广西农业生物科学》 * |
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