CN109239340A - A kind of gel immunodiffusion instrument - Google Patents
A kind of gel immunodiffusion instrument Download PDFInfo
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- CN109239340A CN109239340A CN201811245936.5A CN201811245936A CN109239340A CN 109239340 A CN109239340 A CN 109239340A CN 201811245936 A CN201811245936 A CN 201811245936A CN 109239340 A CN109239340 A CN 109239340A
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- China
- Prior art keywords
- test
- box
- gel
- temperature control
- immunodiffusion
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
Abstract
The invention discloses a kind of gel immunodiffusion instrument, for the special instrument of gel immunodiffusion, effectively improve test efficiency, it include: temperature control box, its underpart is electric calorifie installation, lower part box outer surface is equipped with electronic display, is additionally provided with buzzer siren and timing key, for reminding the time of test；Stainless steel shallow slot is arranged in the top of the electric calorifie installation, puts water-absorption material materials and parts in the stainless steel shallow slot；Box is incubated, bottom is the louver structure of hollow out, and the incubation box has a lid, and when test closes the lid；And several making sheet capsules, it is arranged in the incubation box, the making sheet capsule is the heat-resisting box body of black non transparent, and scale is arranged at bottom, and expands test or single various passes for expanding test suitable for double.This tester guarantee test is gone on smoothly, and avoids in test that agar plate transfer is improved the accuracy of test result, offered convenience for scientific research and teaching repeatedly to pollute.
Description
Technical field
The present invention relates to immunological test field more particularly to a kind of gel immunodiffusion instrument.
Background technique
Soluble antigen and corresponding antibodies are spread in gel, form concentration gradient, are met and concentration in antigen-antibody
Ratio position appropriate forms macroscopic precipitation line or precipitation ring.Common gel has agar, glucan or polyacrylamide
Amine gel.Gel immunodiffusion is divided into single diffusion test and double diffusion test.
By taking agar gel as an example, single diffusion test method is to punch in the agar plate containing specific antibody, and in hole
It is middle that quantitative antigen is added, it is combined after antigen is spread to surrounding with antibody in agar, that is, forms white precipitate ring, diameter
Or area is positively correlated with antigen concentration.Standard curve is made with standard antigen or International Reference albumen simultaneously, that is, it is fixed to can be used to
Amount detects the antigen concentration (mg/ml or U/ml) of non-key sample.
Double diffusion test method is to be added in antigen-antibody respectively in the different corresponding aperture of agar plate, and the two is in gel
Free diffusing forms white precipitate line at the suitable place of ratio.Double expansions can presence to antigen or antibody in antibody titer, sample with
No, antigen-antibody relative amount and its molecular weight etc. are estimated.
Gel immunodiffusion is carried out in teaching at present and research, agar plate is prepared on plate or glass slide, pours into
Pipette is difficult to control when melting agar causes making sheet to fail；When punching on a lbmc agar plate, in order to which control hole is away from need to be with pen flat
Ware or glass slide bottom make marks in advance, both inaccurate, also very cumbersome；Antigen-antibody reaction need to be in the environment of the moisturizing at 37 DEG C
It carries out, and the method generally used at present is that agar plate is placed on to bottom to have in the enamel box of wet blotting paper, enamel box is put
It is placed in 37 DEG C of incubators and incubates 24 or 48 hours, be not used for the incubation device of gel immunodiffusion specifically, tested
Journey seems very cumbersome, and is easy transfer agar plate multiple in operation and easily causes pollution.
Currently, carry out gel immunodiffusion in teaching and research, without special test apparatus, test from making sheet,
Punching is incubated for links such as detections, is needed to prepare a variety of test equipments and is shifted agar plate repeatedly, process is cumbersome and is easy to make
At pollution.
Summary of the invention
The purpose of the present invention is to provide a kind of gel immunodiffusion instrument, needed for providing for gel immunodiffusion
Experimental enviroment, guarantee test goes on smoothly, and improves the accuracy of test result, offers convenience for scientific research and teaching.
For this purpose, be the special instrument of gel immunodiffusion the present invention provides a kind of gel immunodiffusion instrument,
To reduce gel slab transfer number in test, comprising: temperature control box, lower part are equipped with electric calorifie installation, are additionally provided with electronic display, are used for
Temperature and time in show case is additionally provided with buzzer siren and timing key, for reminding the time of test；Stainless steel shallow slot,
The top of the electric calorifie installation is set, storing has a water-absorption material materials and parts, and when test adds water, with inside guarantee test humidity, keep away
Exempt from agar plate to be oven-dried during the experiment；Box being incubated, being completed wherein when for testing for reaction system, bottom is hollow out
Louver structure, the incubation box have a lid, and when test closes the lid, and prevent agar plate moisture evaporation；And several making sheet capsules, if
It sets in the incubation box, for directly preparing gel slab, for directly preparing gel slab, there is scale in the bottom and is applicable in
Yu Shuankuo or single various passes for expanding test, wherein the incubation box is placed directly on water-absorbent material, the blinds knot for incubating box
Structure separates the making sheet capsule with water-absorbent material.
Further, above-mentioned temperature control box has transparent glass chamber door, for optical viewer inner case at any time, the temperature control
Case also has door sensor sealing device.
Further, the top or side of above-mentioned temperature control box are equipped with LED light, light when the chamber door is opened, for seeing
Examine temperature control box inner case.
Further, above-mentioned incubation box is white stainless steel box or enamel box, including box body and box cover.
Further, above-mentioned temperature control box rear and side are equipped with plug and switch.
Further, above-mentioned gel immunodiffusion instrument is gel immunodiffusion special instrument.
Further, above-mentioned electronic display is located at the outer surface of the lower part box of temperature control box, by the electronic display
Side is equipped with temperature control key and timing key.
Further, above-mentioned water-absorption material materials and parts are absorbent wool block.
Further, above-mentioned making sheet capsule be the heat-resisting box body of black non transparent, the bottom have the scale of white marking with
And suitable for double expansions or single various passes for expanding test, when directly preparing gel slab in the making sheet capsule, bottom white hole
Type is used to indicate punch and directly punches, and the precipitation line that the background of black is formed convenient for observation test, scale expands convenient for measurement is single
Test precipitating linear diameter.
Gel immunodiffusion is carried out in teaching at present and research, without special test apparatus, tests from making sheet, beat
Hole is incubated for links such as detections, is needed to prepare a variety of test equipments and is shifted agar plate repeatedly, process is cumbersome and be easy to cause
Pollution provides needed for gel immunodiffusion and owns the present invention provides a kind of gel immunodiffusion special instrument
Demand, guarantee test are gone on smoothly, and avoid in test agar plate transfer improving the standard of test result repeatedly to pollute
True property and test efficiency, offer convenience for scientific research and teaching.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
Below with reference to figure, the present invention is described in further detail.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present invention, and of the invention shows
Examples and descriptions thereof are used to explain the present invention for meaning property, does not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the structural schematic diagram of gel immunodiffusion instrument according to the present invention；
Fig. 2 is the structural schematic diagram of gel immunodiffusion instrument according to the present invention；
Fig. 3 is the structural schematic diagram according to the present invention for incubating box cover；
Fig. 4 is the structural schematic diagram according to the present invention for incubating box body；
Fig. 5 is the structural schematic diagram of making sheet capsule according to the present invention；
Fig. 6 is the schematic diagram of one group of making sheet capsule according to the present invention bottom；And
Fig. 7 shows the schematic diagram of the incubation box according to the present invention that one group of making sheet capsule is housed.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase
Mutually combination.The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
Fig. 1 to Fig. 7 is shown according to some embodiments of the present invention.
In one embodiment, as depicted in figs. 1 and 2, this tester is designed as box (or referred to as cabinet type), i.e. temperature control box
10 (or temperature-controlling cabinets), including cabinet 11 and chamber door 12.
Wherein, chamber door 11 is made of transparent glass, convenient for situation in observation case.Door (door sensor sealing knot when being equipped with magnetic stripe
Structure) 13, when work, shuts chamber door, and the sealing of guarantee test instrument prevents moisture evaporation in case.
The lower part of tester is electric calorifie installation 40, controls the temperature inside the box, and lower part box outer surface is equipped with electronic display 17,
Temperature and time in show case, electronic display side are provided with temperature control key 18 and timing key 19, electronic display side
Equipped with buzzer siren (not shown).Tester rear and side are equipped with plug and switch 15.
Inside tester, the top design of electric calorifie installation is a stainless steel shallow slot 16 (groove), needs to add according to test
Enter a small amount of water.Tester is equipped with some water-absorbent material such as absorbent wool blocks 30, the size and stainless steel groove of the absorbent wool block
It adapts to, is placed in absorbent wool in the groove for containing suitable quantity of water when test.
The space on stainless steel groove top, the antigen-antibody reaction for being placed in tester matching incubate device and incubate
Box 20.
The top or side of tester are equipped with LED light 14, and when opening tester door, LED light is bright, convenient for observation examination
Test instrument inner case.
As shown in Figure 3 and Figure 4, incubating box 20 is white stainless steel or enamel box, including box body 20a and box cover 20b.
As shown in figure 4, the bottom box body 20a is designed as the louver structure 21 of hollow out, and blinds and horizontal plane have certain angle
State for example in a vertical shape is spent, when box body is placed on absorbent wool, bottom will not be bonded with absorbent wool completely, but have certain angle
Degree is stood on absorbent wool, and water is avoided to adhere to other devices in box.
As shown in figure 3, box cover 20b is not connected with box body, when use, is placed on box body, and a handle 22 is designed on box cover,
Convenient for opening.
It is made as shown in figure 5, making sheet capsule 50 is whole of the heat proof material of black non transparent, the making sheet capsule background of black
White precipitate line is formed by convenient for observing after the completion of antigen-antibody is incubated for.
The present invention is by taking agaropectin as an example, when gel immunodiffusion, needs to choose making sheet capsule according to test and is placed in
In large, tester is equipped with the making sheet capsule of different size, according to the needs of agar single diffusion test and agar directional diffusion,
Scale 51a and pass are drawn in the white coating that the bottom 51 of different making sheet capsules all devises adequacy test needs, Fig. 6 is several
The bottom surface of the making sheet capsule of kind specification is adapted to the 5cm scale 51a that single capsule bottom (A) for expanding test only depicts white, suitable
Bottom a capsule (B) that Ying Yushuan expands devises white scale and delthyrium 51b, plum blossom hole (C) 51c and double-row hole (D) 51d
Etc. several passes.
The capsule of selection is placed in large when Fig. 7 is test.This tester configures corresponding Gelose punch.
Test examples one: Simple agar diffusion test
Test principle: antibody and antigen to be measured combine at the suitable position of the two ratio and form precipitation ring.The size of ring
It is positively correlated with the concentration of antigen.
In unidirectional agar gel immunodiffusion test, gel Immune proliferation instrument is opened in advance, the temperature inside the box is set as
37 DEG C, stainless steel shallow slot is interior to be added suitable quantity of water, is put into absorbent wool, so that absorbent wool is kept wet state, and setting time is 24
~48h.
The capsule that white graduation mark is arranged at bottom is chosen, antibody is mixed into 0.9% agarose (50 DEG C), pipettes suitable fine jade
Rouge pours into capsule before not solidifying, and it is about 2~3mm plate that agar, which is frozen into thickness in capsule bottom, needs to compare according to test
The white graduation mark of capsule bottom punches (3~5mm of aperture) with punch, and antigenic solution and difference are added in backward hole to be solidified
The standard items of concentration, cover box cover, will incubate box and are placed in preheated tester, are placed on absorbent wool, due to incubating
The blinds and horizontal plane of large bottom have certain angle, therefore capsule will not contact absorbent wool.
It incubates the antigen-antibody reaction in box and is incubated for 24~48h at 37 DEG C and under wet environment (preventing agar dry).
Whether occur white precipitate ring around peep hole and measures the diameter of ring according to the graduation mark of capsule bottom.And to the concentration of antigen
It is calculated.
Test examples two: double agar diffusion test
Principle: antigen-antibody is added in respectively in the different corresponding aperture of agar gel, the two freely expands in agar gel
It dissipates, forms white precipitate line at the suitable place of ratio.
In double agar diffusion test, gel Immune proliferation instrument is opened in advance, and the temperature inside the box is set as 37 DEG C, it is stainless
Suitable quantity of water is added in steel shallow slot, is put into absorbent wool, absorbent wool is made to keep wet state, and setting time be 18~for 24 hours.
According to the needs of test, the capsule of dimension is selected.
For detecting antibody titer, chooses for plum blossom hole is drawn in bottom and chooses the capsule that white graduation mark is arranged at bottom,
One layer of uniform agar gel thin layer is poured into, is punched after solidification, agar thickness is generally 2~3mm, aperture 3mm, and pitch of holes exists
Between 3~5mm, antigen or antibody are added in corresponding hole, covers box cover, is placed on absorbent wool, due to incubating big cassette bottom
The blinds and horizontal plane in portion have certain angle, therefore capsule will not contact absorbent wool.
By incubate box be placed in preheated tester, incubate 37 DEG C 18~observe afterwards for 24 hours, each self-diffusion in gel
Antigen and antibody can form visible white precipitate line at the appropriate place of concentration ratio.Antibody is judged according to the position that precipitation line occurs
Potency.
Compared with prior art, the invention has the characteristics that/effect:
(1) a kind of Special test instrument for being exclusively used in gel immunodiffusion is designed, avoids shifting agar plate in test
Repeatedly to pollute.
(2) tester includes electric calorifie installation, temperature can be adjusted, and guarantees gel immunodiffusion antigen-antibody
Required temperature when incubation.
(3) tester devises electronic display, to show the temperature during testing and the time of incubation, and designs
Buzzer siren, reminds the time of test.
(4) tester interior design stainless steel shallow slot and water-absorbent material, when test, are added a small amount of water, in guarantee test
The humidity in portion avoids gel from being oven-dried during the test.
(5) tester is configured with incubating device and incubates box, and reaction system is completed in incubating box when test.Incubating device
For enamel or stainless steel material, bottom is the louver structure of hollow out, and louver structure is holded up, and such incubating device can be placed directly in
On water-absorbent material, but not touch the capsule being incubated in box.Being incubated for box has a lid, and when test closes the lid, and prevents gel slab water
Divide volatilization.
(6) it is incubated for the several capsules of setting, capsule in box to be made of the heat proof material of black non transparent, white marking is arranged at bottom
Scale or various passes suitable for double expansions.Gel slab can be directly prepared in capsule, bottom white pass is convenient for beating
Hole device directly punches.The background of black tests the precipitation line formed convenient for observation, and scale is straight convenient for the single expansion test precipitation line of measurement
Diameter.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of gel immunodiffusion instrument is the special instrument of gel immunodiffusion, to reduce gel slab in test
Transfer number characterized by comprising
Temperature control box, lower part are equipped with electric calorifie installation, are additionally provided with electronic display, for temperature and time in show case, are additionally provided with bee
Ring alarm and timing key, for reminding the time of test；
Stainless steel shallow slot, is arranged in the top of the electric calorifie installation, and storing has a water-absorption material materials and parts, and when test adds water, to guarantee to try
It tests internal humidity, agar plate is avoided to be oven-dried during the experiment；
Box being incubated, being completed wherein when for testing for reaction system, bottom is the louver structure of hollow out, and the incubation box has one
Lid, when test, close the lid, and prevent agar plate moisture evaporation；And
Several making sheet capsules are arranged in the incubation box, and for directly preparing gel slab, there is scale in the bottom and is applicable in
Yu Shuankuo or single various passes for expanding test,
Wherein, which is placed directly on water-absorbent material, and the louver structure for incubating box is by the making sheet capsule and absorbs water
Material separates.
2. gel immunodiffusion instrument according to claim 1, which is characterized in that the temperature control box has transparent glass
Chamber door, for optical viewer inner case, the temperature control box also to have door sensor sealing device at any time.
3. gel immunodiffusion instrument according to claim 2, which is characterized in that the top or side of the temperature control box
Equipped with LED light, lighted when the chamber door is opened, for observing temperature control box inner case.
4. gel immunodiffusion instrument according to claim 1, which is characterized in that the incubation box is white stainless steel
Box or enamel box, including box body and box cover.
5. gel immunodiffusion instrument according to claim 1, which is characterized in that the temperature control box rear and side dress
There are plug and switch.
6. gel immunodiffusion instrument according to claim 1, which is characterized in that the water-absorption material materials and parts are absorbent wool
Block.
7. gel immunodiffusion instrument according to claim 1, which is characterized in that the electronic display is located at temperature control
The outer surface of the lower part box of case, the electronic display side are equipped with temperature control key.
8. gel immunodiffusion instrument according to claim 1, which is characterized in that the making sheet capsule is that black is impermeable
Bright heat-resisting box body, there is the scale of white marking in the bottom and suitable for double expansions or single various pass for expanding test, described
When directly preparing gel slab in making sheet capsule, bottom white pass is used to indicate punch and directly punches, and the background of black is convenient for
The precipitation line that observation test is formed, scale expand test precipitating linear diameter convenient for measurement is single.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811245936.5A CN109239340A (en) | 2018-10-24 | 2018-10-24 | A kind of gel immunodiffusion instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811245936.5A CN109239340A (en) | 2018-10-24 | 2018-10-24 | A kind of gel immunodiffusion instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109239340A true CN109239340A (en) | 2019-01-18 |
Family
ID=65081716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811245936.5A Pending CN109239340A (en) | 2018-10-24 | 2018-10-24 | A kind of gel immunodiffusion instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109239340A (en) |
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2018
- 2018-10-24 CN CN201811245936.5A patent/CN109239340A/en active Pending
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