CN109239336A - A kind of test strips and its application detecting Mobucin - Google Patents
A kind of test strips and its application detecting Mobucin Download PDFInfo
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- CN109239336A CN109239336A CN201811093317.9A CN201811093317A CN109239336A CN 109239336 A CN109239336 A CN 109239336A CN 201811093317 A CN201811093317 A CN 201811093317A CN 109239336 A CN109239336 A CN 109239336A
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a kind of test strips and its application for detecting Mobucin.Test strips include sample absorption pad (1), conjugate release pad (2), reaction film (3), water absorption pad (4) and bottom plate (7), have on the reaction film and be coated with the detection line (5) of Mobucin hapten-carrier protein conjugate and be coated with the nature controlling line (6) of sheep anti mouse antiantibody, the conjugate release pad (2) is coated with Mobucin monoclonal antibody-colloid gold label object.The present invention also provides the remaining methods of Mobucin in a kind of crops such as the above-mentioned Mobucin test strips detection veterinary antibiotics of application.Test strips provided by the present invention have the characteristics that easy to operate, high sensitivity, detection speed are fast, at low cost, are suitble to the screening and on-site supervision of great amount of samples.
Description
Technical field
The present invention relates to a kind of test strips and its application for detecting Mobucin, and in particular to a kind of for detecting Mobucin
Colloidal gold strip, it is especially suitable for the remaining detections of Mobucin in the crops such as veterinary antibiotics.
Background technique
Mobucin also known as go out is flutterred scattered, Ye Chan and is dissipated, and chemical name is adjacent propyl phenol methylcarbamate.The quality 1970
Recommended in year to be used as insecticide by Germany Bayer company, there is stronger action of contace poison, the performance in prevention and treatment rice grub
Important function.In recent years, usage amount of the Mobucin in the crops such as veterinary antibiotics increasingly increases, and which results in farming produce
The persticide residue of product is higher, is detrimental to health.
Currently, the method for detection Mobucin medicament residue is mostly instrumental method, more difficult, higher cost is operated, the present invention is
Rapid detection method, the used time is short, easy to operate, and cost is relatively low, is suitable for grass-roots unit's test sample in large quantity.
Summary of the invention
The object of the present invention is to provide a kind of high sensitivities, the Mobucin residual that easy to operate, at low cost, detection time is short
Test strip.
The remaining test strips of detection Mobucin provided by the present invention, including sample absorption pad (1), conjugate release pad
(2), reaction film (3), water absorption pad (4) and bottom plate (7)；Have on the reaction film and is coated with Mobucin hapten-carrier albumen
The detection line (5) of conjugate and the nature controlling line (6) for being coated with sheep anti mouse antiantibody, the conjugate release pad (2) are coated with different
Third prestige monoclonal antibody-colloid gold label object.
The Mobucin hapten-carrier protein conjugate is to be obtained by Mobucin haptens with carrier protein couplet, institute
Stating carrier protein is bovine serum albumin(BSA), ovalbumin, hemocyanin, thyroprotein, human serum albumins.
The Mobucin monoclonal antibody is to prepare to obtain using Mobucin hapten-carrier protein conjugate as immunogene
, it is to be secreted to obtain by Mobucin monoclonal antibody hybridoma cell strain；The sheep anti mouse antiantibody is by source of mouse antibody mediated immunity
Sheep obtains.
The sample absorption pad (1), conjugate release pad (2), reaction film (3), water absorption pad (4) are successively pasted onto bottom plate
(7) on, the conjugate release pad 1/3~1/2 is capped under sample absorption pad.
The bottom plate can be the material that PVC bottom plate or other hard do not absorb water；The sample absorption pad can for suction strainer paper or
Filter paper for oil；The conjugate release pad can be mineral wool or polyester material；The water absorption pad is blotting paper；The reaction film can be
Nitrocellulose filter or cellulose acetate film.
It is a further object to provide a kind of methods for preparing above-mentioned test strips comprising step:
1) preparation is coated with Mobucin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with Mobucin hapten-carrier protein conjugate and is coated with sheep anti mouse and resists
The reaction film of the nature controlling line of body；
And 2) 3) 1) the conjugate release pad, reaction film and sample absorption pad, water absorption pad and the bottom plate that prepare are assembled into
Test strips.
Specifically, step includes:
1) prepared by haptens: the substances such as 4- amino -2- isopropyl-phenol and di-tert-butyl dicarbonate are passed through a series ofization
It learns reaction and obtains Mobucin haptens；
2) by Mobucin haptens and carrier protein couplet, Mobucin hapten-carrier protein conjugate is obtained；
3) mouse is immunized with Mobucin hapten-carrier protein conjugate, mouse boosting cell and myeloma cell is passed through
Fusion, screening, obtain Mobucin monoclonal hybridoma strain；
4) mouse IgG immune health goat is extracted, sheep anti mouse antiantibody is obtained；
5) it is reacted with trisodium citrate with gold chloride and prepares colloidal gold；
6) the Mobucin monoclonal antibody by step 3) preparation is added in the colloidal gold of step 5) preparation, obtains Mobucin list
Clonal antibody-colloid gold label object；
7) Mobucin monoclonal antibody-colloid gold label object is sprayed in conjugate release pad, is taken out after 37 DEG C of baking 1h,
It is placed in dry environment and saves backup；
8) Mobucin hapten-carrier protein conjugate is coated on reaction film and constitutes detection line, sheep anti mouse is anti-
Body, which is coated on reaction film, constitutes nature controlling line；
9) sample absorption pad is used and contains 0.5% bovine serum albumin(BSA) (volume fraction), pH 7.2,0.1mol/L phosphate
Buffer impregnates 2h, dries 2h at 37 DEG C；
10) upper sample absorption pad, conjugate release pad, reaction film, water absorption pad are pasted in order on bottom plate, sample absorbs
Pad covers conjugate release pad, is finally cut into the small item of 3mm wide, adds plastic casing, is vacuum-packed, and can be reserved under the conditions of 4~30 DEG C
12 months.
It is a further object to provide Mobucin in a kind of above-mentioned test strips detection vegetables of application and crops is residual
The method stayed, it comprising steps of
(1) sample pre-treatments；
(2) it is detected with test strips；
(3) analysis detection result.
Mobucin Rapid detection test strip of the invention is exempted from using the antibody antigen reaction of high degree of specificity and Competitive assays
Mobucin monoclonal antibody-colloid gold label object is fixed in conjugate release pad by epidemic disease Chromatographic techniques, different in sample
Third prestige is in flow process, in conjunction with Mobucin monoclonal antibody-colloid gold label object in conjugate release pad, forms medicine
Object-antibody-colloidal gold marker.The Mobucin hapten-carrier albumen coupling on drug and reaction film detection line in sample
Object competitive binding Mobucin monoclonal antibody-colloid gold label object, according to detection line red stripes whether there is or not or shade sentence
Whether remained containing Mobucin in disconnected analyte sample fluid.
When detection, sample is instilled after processing in test strips hole, when the concentration of Mobucin in the sample lower than detection limit or
When being zero, monoclonal antibody-colloid gold label object meeting and the Mobucin haptens-load being fixed on reaction film in chromatography process
Body protein conjugate combines, and a red stripes respectively occurs in detection line (T) and nature controlling line (C)；If Mobucin is in sample
In concentration be equal to or higher than detection limit, monoclonal antibody-colloid gold label object can with Mobucin all be combined, thus in T line
Place is because competitive reaction will not be in conjunction with Mobucin hapten-carrier protein conjugate without there are red stripes.
Test strips of the invention have that high sensitivity, high specificity, at low cost, easy to operate, detection time is short, it is each to be suitble to
The advantages of kind unit uses, storage is simple, long shelf-life.It is easy, fast with the remaining method of test strips of the present invention detection Mobucin
Fast, intuitive, accurate, applied widely, at low cost, easy popularization and use.
Detailed description of the invention
Fig. 1 is Mobucin hapten synthesis figure.
Fig. 2 is test strips the schematic diagram of the section structure.
Specific embodiment
Below with reference to specific embodiment, the present invention is further explained.It should be understood that these embodiments are merely to illustrate this
Invention, and be not intended to limit the scope of the invention.
The preparation of 1 Mobucin test strip of embodiment
The preparation method of the test strips mainly comprises the steps that
1) preparation is coated with Mobucin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with Mobucin hapten-carrier protein conjugate and is coated with sheep anti mouse and resists
The reaction film of the nature controlling line of body；
And 2) 3) 1) conjugate release pad, reaction film and the sample absorption pad, water absorption pad and PVC bottom plate that prepare are assembled
At test strips.
Substep narration in detail below:
1, the preparation of Mobucin haptens
(1) 4- amino -2- isopropyl-phenol 1.51g is taken, the 50ml that adds methylene chloride dissolution adds di-tert-butyl dicarbonate
4h is stirred at room temperature in 2.18g, triethylamine 0.4ml, stops reaction, and revolving removes organic solvent, adds water 30ml, ethyl acetate 40ml
× 3 extractions three times, merge organic phase, are evaporated, and methylene chloride/n-hexane (v/v, 1/7) 90ml recrystallization obtains chemical compounds I
2.3g, yield 92%；
(2) chemical compounds I 2.3g is taken, anhydrous propanone 60ml is added to dissolve, 0-5 DEG C, methyl isocyanate 0.73g is added dropwise, adds pyrrole
Pyridine 1.2ml after being sufficiently stirred, is restored to room temperature, continues to stir 3h；Revolving removes organic solvent, upper silicagel column, petroleum ether/second
Acetoacetic ester (v/v, 5/1) elution separation, obtains compound ii 2.7g, yield 96.43%；
(3) compound ii 2.7g is taken, adds trifluoroacetic acid 40ml to dissolve, 6h is stirred at room temperature；Stop reaction, revolving removes molten
Agent, chlorination imitate 100ml, and dissolution clarification, three times, organic phase anhydrous sodium sulfate is dry for washing, is evaporated, ethyl acetate/n-hexane (v/
V, 1/10) 120ml recrystallization, obtain amino Mobucin haptens product 1.4g, yield 77%.
2, the preparation of immunogene
Amino Mobucin haptens 50mg is taken, 1mol/L hydrochloric acid is added, 0.48ml adds water 5ml, stirring and dissolving, clarification, 0-5
DEG C stirring 30min, add sodium nitrite 17.2mg, continue stir 3h, obtain activating solution A liquid；Bovine serum albumin(BSA) 500mg is taken, is added
0.1mol/L sodium hydroxide solution 30ml dissolution, 0-5 DEG C of stirring 30min obtain B liquid, and whole A drops is added in B liquid, is stirred
Mix 4h.0.02mol/L PB buffer is dialysed three days, is changed liquid 3 times daily, is obtained Mobucin-BSA immunogene, -20 DEG C of preservations are standby
With.
3, the preparation of coating antigen
Amino Mobucin haptens 30mg is taken, 1mol/L hydrochloric acid is added, 0.27ml adds water 5ml, stirring and dissolving, clarification, 0-5
DEG C stirring 30min, add sodium nitrite 13.1mg, continue stir 3h, obtain activating solution A liquid；Ovalbumin 500mg is taken, is added
0.1mol/L sodium hydroxide solution 30ml dissolution, 0-5 DEG C of stirring 30min obtain B liquid, and whole A drops is added in B liquid, is stirred
Mix 4h.0.02mol/L PB buffer is dialysed three days, is changed liquid 3 times daily, is obtained Mobucin-OVA coating antigen, -20 DEG C of preservations are standby
With.
4, the preparation of Mobucin monoclonal antibody
(1) animal immune
In the immunogene injection Balb/c Mice Body that step 2 is obtained, immunizing dose is 150 μ g/, its is made to generate anti-blood
Clearly.
(2) cell fusion and cloning
Immune Balb/c mouse boosting cell is taken, is merged in 8:1 (quantitative proportion) ratio with SP2/0 myeloma cell, is used
Indirect competitive ELISA method measures cell supernatant, screens positive hole.Cloning is carried out to positive hole using limiting dilution assay, directly
To obtaining the hybridoma cell strain of stably excreting monoclonal antibody.
(3) cell cryopreservation and recovery
Hybridoma is made 1 × 10 with frozen stock solution6The cell suspension of a/ml, saves for a long time in liquid nitrogen.When recovery
Cryopreservation tube is taken out, 37 DEG C of water-bath middling speeds is immediately placed in and melts, after centrifugation removal frozen stock solution, moves into culture culture in glassware.
(4) preparation and purification of monoclonal antibody
Increment cultivation: hybridoma is placed in cell culture medium, is cultivated under the conditions of 37 DEG C, with octanoic acid-
Saturated ammonium sulfate method purifies obtained culture solution, obtains monoclonal antibody, -20 DEG C of preservations.
The cell culture medium is that calf serum and sodium bicarbonate are added into RPMI1640 culture medium, and calf serum is made to exist
Final concentration of 20% (mass fraction) in cell culture medium, the final concentration of 0.2% (matter of sodium bicarbonate in cell culture medium
Measure score)；The pH of the cell culture medium is 7.4.
5, the preparation of sheep anti mouse antiantibody
Using sheep as immune animal, pathogen-free domestic sheep is immunized using source of mouse antibody as immunogene, it is anti-to obtain sheep anti mouse
Antibody.
6, Mobucin monoclonal antibody-colloid gold label object preparation
(1) preparation of colloidal gold
1% gold chloride is diluted to 0.01% (mass fraction) with double distilled deionized water, 100ml is taken to be placed in conical flask,
It is heated to boiling with thermostatic electromagnetic blender, in continuous high temperature, is persistently added with stirring 1% trisodium citrate of 2.5ml, continue
Solution is at the uniform velocity heated with stirring in stopping when bright red, is restored to original volume, 4 DEG C of guarantors with deionized water after being cooled to room temperature
It deposits.The colloidal gold appearance prepared is pure, it is bright, without precipitating and floating material.
(2) Mobucin monoclonal antibody-colloid gold label object preparation
Under magnetic stirring, molten by every milliliter of colloidal gold with the pH value of 0.2mol/L solution of potassium carbonate tune colloidal gold to 7.0
Mobucin monoclonal antibody is added into colloidal gold solution for the standard that 20~50 μ g are added in liquid, continues to stir and evenly mix 30min, add
Enter 10%BSA, make its final concentration of 1% (volume fraction) in colloidal gold solution, stands 10min.12000r/min,4℃
It is centrifuged 40min, abandons supernatant, it is the redissolution of initial colloid gold volume 1/10 with volume that precipitating is washed twice with redissolution buffer
Buffer will precipitating be resuspended, set 4 DEG C it is spare.
Redissolve buffer: casein containing protein 0.02%~0.1% (mass fraction), 0.05%~0.2% (quality of Tween-80
Score), the 0.02mol/L phosphate buffer of pH7.2.
7, the preparation of conjugate release pad
Conjugate release pad is soaked in (concentration of the bovine serum albumin(BSA) in buffer is containing bovine serum albumin(BSA)
0.5%), in the phosphate buffer of pH 7.2,0.5mol/L, 1h is uniformly soaked, 37 DEG C of baking 3h are spare.Film is sprayed with Isoflow
Instrument by the Mobucin prepared monoclonal antibody-colloid gold label object even application in conjugate release pad, every 1cm conjugate
After release pad sprays 0.01ml Mobucin monoclonal antibody-colloid gold label object, (humidity < 20%) is placed in 37 DEG C of environment
It is taken out after 60min, is placed in dry environment (humidity < 20%) and saves backup.
8, the preparation of reaction film
Detection line will be constituted in Mobucin haptens-ovalbumin conjugate coating to reaction film, by sheep anti mouse antiantibody
It is coated on reaction film and constitutes nature controlling line.
Coating process: being diluted to 10mg/ml for Mobucin haptens-ovalbumin conjugate with phosphate buffer, uses
Isoflow point film instrument is coated in the detection line (T line) on nitrocellulose filter, and package amount is 0.8 μ l/cm；With
Sheep anti mouse antiantibody is diluted to 200 μ g/ml by the phosphate buffer of 0.01mol/L, pH7.4, with Isoflow point film instrument by its
The nature controlling line (C line) being coated on nitrocellulose filter, package amount are 1.0 μ l/cm.The reaction film being coated with is placed in 37 DEG C of items
Dry 2h, spare under part.
9, the preparation of sample absorption pad
Sample absorption pad is placed in slow containing 0.5% bovine serum albumin(BSA) (volume fraction), pH7.2,0.1mol/L phosphate
2h is impregnated in fliud flushing, 37 DEG C of baking 2h are spare.
10, the assembling of test strips
Sample absorption pad, conjugate release pad, reaction film, water absorption pad are successively pasted on PVC bottom plate in order；In conjunction with
Object release pad has 1/3 region to be absorbed by the sample pad covering from starting point, and the end of conjugate release pad and the beginning of reaction film connect
It connects, the end of reaction film is connected with the beginning of water absorption pad, and the beginning of sample absorption pad is aligned with the beginning of PVC bottom plate, water absorption pad
End be aligned with the end of PVC bottom plate；There are detection line and nature controlling line, detection line (T line) and nature controlling line (C on the reaction film
Line) it is the strip tape perpendicular with the length of the test strips；Detection line is located at the side close to the end of conjugate release pad；
Nature controlling line is located remotely from the side of the end of conjugate release pad；Test strips are cut into the small item of 3mm wide with machine, mounted in special
Plastics fabrication in, under the conditions of 4~30 DEG C can be reserved for 12 months.
The remaining detection of Mobucin in 2 sample of embodiment
1, it is detected with test strips
Measuring samples solution, which to be drawn, with suction pipe 3 drops is vertically added dropwise in well, liquid starts timing when flowing, reaction 5~
10min determines result.
2, analysis detection result
Result is read by colloidal gold analyzer (referred to as " analyzer "):
Negative (-): indicate that test substance concentration is lower than detection limit in sample；
Positive (+): indicate that test substance concentration is equal to or higher than detection limit in sample；
It is invalid: to indicate to need to retest.
3 sample detection example of embodiment
1, detection limit test
Blank Chinese cabbage and apple sample are taken, addition Mobucin takes examination to final concentration of 5,10,20 μ g/kg respectively wherein
Paper slip is detected, and each sample is repeated three times.
When detecting Chinese cabbage and apple sample with test strips, when wherein Mobucin addition concentration is 5 μ g/kg, analyzer is aobvious
It is shown as negative；When wherein Mobucin addition concentration is 10,20 μ g/kg, analyzer is shown as positive.
2, false positive rate, false negative rate test
Take the Chinese cabbage and each 20 parts of apple positive sample and content that known Mobucin content is 10 μ g/kg less than 5 μ g/kg's
Chinese cabbage and each 20 parts of apple negative sample, are detected with three batches of test strips, calculate its yin and yang attribute rate.It the results are shown in Table 1, table 2.
1 Chinese cabbage of table detects sample results
2 apple of table detects sample results
The result shows that: when detecting positive Chinese cabbage and apple sample with the test strips of 3 batch productions, as a result it is all positive,
Know that positive sample coincidence rate is 100%, false negative rate 0；When detecting 20 parts of negative Chinese cabbages and apple sample, it is as a result all yin
Property, it is known that negative match-rate 100%, false positive rate 0.Illustrate that the test strips of detection Mobucin of the invention can be to Chinese cabbage
And Mobucin residual is used for quickly detecting in apple.
3, specific test
The drugs such as 500 μ g/kg alachlors, imines bacterium, diethofencarb and delachlor are detected with Mobucin test strips.As a result it shows
Show, test strips nature controlling line and detection line develop the color, and are negative.Illustrate that this test strips is mould to 500 μ g/kg alachlors, imines bacterium, second
The drugs no cross reaction such as prestige and delachlor.
Claims (8)
1. a kind of test strips for detecting Mobucin, including sample absorption pad (1), conjugate release pad (2), reaction film (3), water suction
Pad (4) and bottom plate (7), it is characterised in that have on the reaction film and be coated with Mobucin hapten-carrier protein conjugate
Detection line (5) and the nature controlling line (6) for being coated with sheep anti mouse antiantibody, the conjugate release pad (2) are coated with Mobucin Dan Ke
Grand antibody-colloidal gold marker, the Mobucin haptens the preparation method is as follows:
(1) 4- amino -2- isopropyl-phenol 1.51g is taken, the 50ml that adds methylene chloride dissolution adds di-tert-butyl dicarbonate 2.18g, and three
4h is stirred at room temperature in ethamine 0.4ml, stops reaction, and revolving removes organic solvent, and water 30ml, ethyl acetate 40ml × 3 is added to extract
Three times, merge organic phase, be evaporated, methylene chloride/n-hexane (v/v, 1/7) 90ml recrystallization obtains chemical compounds I 2.3g, yield
92%；
(2) chemical compounds I 2.3g is taken, anhydrous propanone 60ml is added to dissolve, 0-5 DEG C, methyl isocyanate 0.73g is added dropwise, adds pyridine
1.2ml after being sufficiently stirred, is restored to room temperature, continues to stir 3h；Revolving removes organic solvent, upper silicagel column, petroleum ether/acetic acid
Ethyl ester (v/v, 5/1) elution separation, obtains compound ii 2.7g, yield 96.43%；
(3) compound ii 2.7g is taken, adds trifluoroacetic acid 40ml to dissolve, 6h is stirred at room temperature；Stop reaction, revolving removes solvent, adds
Chloroform 100ml, dissolution clarification, three times, organic phase anhydrous sodium sulfate is dry for washing, is evaporated, and ethyl acetate/n-hexane (v/v, 1/
10) 120ml is recrystallized, and obtains amino Mobucin haptens product 1.4g, yield 77%.
2. test strips as described in claim 1, it is characterised in that the sample absorption pad (1), conjugate release pad (2), anti-
Film (3), water absorption pad (4) is answered successively to be pasted on bottom plate (7).
3. such as the described in any item test strips of claim 1-2, it is characterised in that the conjugate release pad 1/3~1/2 is coating
It is placed under sample absorption pad.
4. test strips as described in claim 1, it is characterised in that the Mobucin hapten-carrier protein conjugate is by isopropyl
Prestige haptens is obtained with carrier protein couplet, and the carrier protein is bovine serum albumin(BSA), ovalbumin, hemocyanin, first shape
Gland albumen, human serum albumins.
5. such as the described in any item test strips of claim 1 or 4, it is characterised in that the Mobucin haptens is by 4- amino-
The substances such as 2- isopropyl-phenol and di-tert-butyl dicarbonate are obtained by series of chemical, molecular structural formula are as follows:
6. test strips as described in claim 1, it is characterised in that the Mobucin monoclonal antibody is with Mobucin haptens-
Carrier protein couplet object is prepared as immunogene, and the sheep anti mouse antiantibody is to obtain source of mouse antibody mediated immunity sheep.
7. a kind of method for preparing any one of claim 1-6 test strips comprising step:
1) preparation is coated with Mobucin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with Mobucin hapten-carrier protein conjugate and is coated with sheep anti mouse antiantibody
The reaction film of nature controlling line；
And 2) 3) 1) the conjugate release pad, reaction film and sample absorption pad, water absorption pad and the bottom plate that prepare are assembled into test paper
Item.
8. the remaining method of Mobucin in a kind of crops such as detection veterinary antibiotics comprising step:
1) Sample pretreatment；
2) it is detected with test strips described in any one of claims 1-6；
3) analysis detection result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811093317.9A CN109239336B (en) | 2018-09-19 | 2018-09-19 | Test strip for detecting isoprocarb and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811093317.9A CN109239336B (en) | 2018-09-19 | 2018-09-19 | Test strip for detecting isoprocarb and application thereof |
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| CN109239336B CN109239336B (en) | 2023-02-24 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111896738A (en) * | 2020-08-03 | 2020-11-06 | 北京望尔生物技术有限公司 | Test strip for detecting serpentrin and application thereof |
| CN112067812A (en) * | 2020-08-26 | 2020-12-11 | 北京勤邦生物技术有限公司 | Test strip for detecting polychlorinated biphenyl and application thereof |
| CN112462070A (en) * | 2020-11-13 | 2021-03-09 | 北京望尔生物技术有限公司 | Test strip for detecting alachlor and application thereof |
| CN113759110A (en) * | 2021-10-18 | 2021-12-07 | 云南省烟草质量监督检测站 | Test strip for detecting propamocarb and application thereof |
| CN116425880A (en) * | 2023-04-04 | 2023-07-14 | 北京纳百生物科技有限公司 | Anti-isoprocarb monoclonal antibody, kit and application |
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