CN110441518A - A kind of test strips and method detecting gibberellin - Google Patents
A kind of test strips and method detecting gibberellin Download PDFInfo
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of test strips and method for detecting gibberellin.The test strips include sample absorption pad, conjugate release pad, reaction film, water absorption pad and bottom plate, have on the reaction film and be coated with the detection line of gibberellin hapten-carrier protein conjugate and be coated with the nature controlling line of sheep anti mouse antiantibody, gibberellin monoclonal antibody-colloid gold label object is coated in the conjugate release pad.The present invention also provides a kind of methods using gibberellin in above-mentioned test strips test sample.Test strips provided by the invention have many advantages, such as easy to operate, high sensitivity, detection speed it is fast, it is at low cost, be suitable for the screening of high-volume sample, can satisfy foods supervision department, China carry out on-site supervision and detection work.
Description
Technical field
The present invention relates to a kind of test strips and method for detecting gibberellin, and in particular to a kind of for detecting the glue of gibberellin
Body gold test paper strip, it is especially suitable for the remaining detections of gibberellin in bean sprouts.
Background technique
Gibberellin is a kind of broad spectrum activity plant growth regulator, is almost all played in each stage of plant growth, development
Adjustment effect especially has significant effect of increasing production to vegetable and fruit etc..Gibberellin is widely used today as the growth of rootless bean sprouts
Regulator.Human body, which takes in excessive gibberellin, may interfere with the endocrine system of human normal, and long-term consumption can accumulate in human body,
The slow poisoning of organ is caused, and may cause canceration, seriously endangers the physical and mental health of consumer.China's original state food drug
Supervision and management general bureau, the Ministry of Agriculture, national health and Family Planning Committee are about being forbidden to use gibberellin in the production process of bean sprouts
Regulation in the bulletin (o.11 in 2015) of equal substances: the producer must not use gibberellin, 4- chlorobenzene in the production process of bean sprouts
The substances such as Fratol, 6-benzyladenine, bean sprouts operator must not manage containing gibberellin, 4-chlorophenoxyacetic acid sodium, 6- benzyl
The bean sprouts of the substances such as base adenine.
The method of detection gibberellin reported at present is mainly the instruments such as liquid chromatography, liquid chromatography tandem mass spectrometry
Method.These methods must operate in laboratory conditions, and sample pre-treatments are cumbersome time-consuming, and the instrument for being equipped with valuableness is also needed to set
Standby, testing cost is higher, time-consuming, complicated for operation, has in actual application very big restricted, it is difficult to meet a large amount of samples
The needs that product and field sample quickly detect.Therefore, develop it is a kind of it is simple and quick, be suitable for the remaining colloidal gold of gibberellin in bean sprouts
Test strips can meet a large amount of sample site screenings and monitoring, can preferably meet the developments such as foods supervision department, China detection
Work.
Summary of the invention
The purpose of the present invention is to provide one kind to be able to detect the remaining colloidal gold strip of gibberellin in bean sprouts, and mentions
For a kind of efficient, accurate, simplicity, suitable for the detection method of on-site supervision and great amount of samples screening.
It is provided by the present invention detection gibberellin test strips, including sample absorption pad, conjugate release pad, reaction film,
Water absorption pad and bottom plate；There is the detection line and coating for being coated with gibberellin hapten-carrier protein conjugate on the reaction film
There is the nature controlling line of sheep anti mouse antiantibody；Gibberellin monoclonal antibody-colloid gold label object is coated in the conjugate release pad.
The gibberellin monoclonal antibody is to prepare to obtain using gibberellin hapten-carrier protein conjugate as immunogene
.
The gibberellin hapten-carrier protein conjugate is obtained by gibberellin haptens with carrier protein couplet, described
Carrier protein is bovine serum albumin(BSA), ovalbumin, hemocyanin, thyroprotein or human serum albumins, the gibberellin
Haptens is to react to obtain with butyric acid hydroxyl oxime again after being aoxidized by gibberellin, molecular structural formula are as follows:
The sample absorption pad, conjugate release pad, reaction film, water absorption pad are successively pasted on bottom plate, the conjugate
Release pad 1/3~1/2 is capped under sample absorption pad.
The bottom plate can be the material that PVC bottom plate or other hard do not absorb water；The sample absorption pad can for suction strainer paper or
Filter paper for oil；The conjugate release pad can be mineral wool or polyester material；The water absorption pad is blotting paper；The reaction film can be
Nitrocellulose filter or cellulose acetate film.
It is a further object to provide a kind of methods for preparing above-mentioned test strips comprising step:
1) preparation is coated with gibberellin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with gibberellin hapten-carrier protein conjugate and is coated with sheep anti mouse and resists
The reaction film of the nature controlling line of body；
And 2) 3) 1) the conjugate release pad, reaction film and sample absorption pad, water absorption pad and the bottom plate that prepare are assembled into
Test strips.
Specifically, step includes:
1) it is reacted again with butyric acid hydroxyl oxime after aoxidizing gibberellin, prepares gibberellin haptens；
2) by gibberellin haptens and carrier protein couplet, gibberellin hapten-carrier protein conjugate is prepared；
3) mouse is immunized with gibberellin hapten-carrier protein conjugate, by mouse boosting cell and murine myeloma cell
By merging, screening, the hybridoma cell strain of secretion gibberellin monoclonal antibody is obtained；
4) mouse IgG immune health goat is extracted, sheep anti mouse antiantibody is obtained；
5) gibberellin hapten-carrier protein conjugate and sheep anti mouse antiantibody are coated in the detection line of reaction film respectively
(T) and on nature controlling line (C)；
6) it is reacted with trisodium citrate with gold chloride and prepares colloidal gold；
7) the gibberellin monoclonal antibody of preparation is added in the colloidal gold of preparation, obtains gibberellin monoclonal antibody-
Colloid gold label object；
8) gibberellin monoclonal antibody-colloid gold label object is sprayed in conjugate release pad, is taken out after 37 DEG C of baking 1h,
It is placed in dry environment and saves backup；
9) phosphate buffer containing 0.5% bovine serum albumin(BSA), pH 7.2,0.1mol/L is used to soak sample absorption pad
2h is steeped, dries 2h at 37 DEG C；
10) upper sample absorption pad, conjugate release pad, reaction film, water absorption pad are pasted in order on bottom plate, conjugate is released
Put pad has 1/3 region to be absorbed by the sample pad covering from starting point.It is finally cut into the small item of 3mm wide, adds plastic casing, is vacuum-packed, 4
It is saved 12 months under the conditions of~30 DEG C.
It is a further object to provide a kind of above-mentioned test strips of application to detect the remaining method of gibberellin in bean sprouts,
It comprising steps of
(1) sample pre-treatments；
(2) it is detected with test strips；
(3) analysis detection result.
Gibberellin Rapid detection test strip of the invention is using the antibody antigen reaction of high degree of specificity and immunochromatography point
Gibberellin monoclonal antibody-colloid gold label object is fixed in conjugate release pad by analysis technology, and the gibberellin in sample is flowing
During dynamic in conjunction with gibberellin monoclonal antibody-colloid gold label object in conjugate release pad, drug-antibody-glue is formed
Body gold marker.The gibberellin hapten-carrier protein conjugate competitive binding on drug and reaction film detection line in sample
Gibberellin monoclonal antibody-colloid gold label object judges whether contain in analyte sample fluid according to the detection line red stripes depth
There is gibberellin residual.
When detection, sample is instilled after processing in test strips card hole, when the concentration of gibberellin in the sample is lower than detection limit
Or when being zero, monoclonal antibody-colloid gold label object meeting and the gibberellin haptens-being fixed on reaction film in chromatography process
Carrier protein couplet object combines, one red stripes of each appearance in detection line (T) and nature controlling line (C), and the colour developing of T line is than C line
Colour developing is deep or consistent with the colour developing of C line；If the concentration of gibberellin in the sample is equal to or higher than detection limit, monoclonal antibody-glue
Body gold marker can all be combined with gibberellin, thus because competitive reaction will not be with gibberellin hapten-carrier egg at T line
White conjugate is combined without red stripes occur or developing the color than C line shallow.As shown in Figure 3.
It is negative: when nature controlling line (C) shows that red stripes, detection line (T) also show that red stripes, and (T) line simultaneously
When color is close or is deeper than (C) line, it is judged to feminine gender.
Positive: when nature controlling line (C) shows red stripes, and detection line (T) does not develop the color or (T) line color is shallower than (C) line
When, it is judged to the positive.
It is invalid:, should whether nature controlling line (C) does not show red stripes, then no matter detection line (T) shows red stripes
Test strips are judged in vain.
Test strips of the invention have that high sensitivity, high specificity, at low cost, easy to operate, detection time is short, it is each to be suitble to
The advantages of kind unit uses, storage is simple, long shelf-life.It is easy, fast with the remaining method of test strips of the present invention detection gibberellin
Fast, intuitive, accurate, applied widely, at low cost, easy popularization and use.
Detailed description of the invention
Fig. 1 is gibberellin hapten synthesis figure.
Fig. 2 is test strips the schematic diagram of the section structure.
Fig. 3 is test strips testing result process decision chart.
Specific embodiment
Below with reference to specific embodiment, the present invention is further explained.It should be understood that these embodiments are merely to illustrate this
Invention, and be not intended to limit the scope of the invention.
Embodiment 1 detects the preparation of the test strips of gibberellin
The preparation method of the test strips mainly comprises the steps that
1) preparation is coated with gibberellin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with gibberellin hapten-carrier protein conjugate and is coated with sheep anti mouse and resists
The reaction film of the nature controlling line of body；
And 2) 3) 1) the conjugate release pad, reaction film and sample absorption pad, water absorption pad and the bottom plate that prepare are assembled into
Test strips.
Substep narration in detail below:
1, the synthesis of gibberellin haptens (synthetic route is shown in attached drawing 1)
Gibberellin 0.35g is taken, is added in the ozone water solution of 50mL0.24mg/mL, 1h is sufficiently stirred, by the reaction solution
It is added in the solution containing zinc powder 1.34g and 1mL acetic acid, continues to stir 2h, stop reaction, filtering removes zinc powder, and filtrate adds
Ethyl acetate 120mL, water 50mL are extracted, separation, and organic phase washing, concentration is evaporated, methylene chloride/n-hexane (V/V, 1/5)
65mL recrystallization obtains oxidation gibberellin 0.18g, yield 51.42%；
Oxidation gibberellin 0.18g is taken, ethyl alcohol 50mL is added to dissolve, clarification adds butyric acid hydroxyl oxime 0.12g, trichloroacetic acid 0.2mL,
3h, stopping reaction being stirred at room temperature, revolving removes ethyl alcohol, adds water 60mL, and add methylene chloride 80mL, and extraction divides and goes water phase, organic
It is mutually evaporated, upper silicagel column, petrol ether/ethyl acetate (V/V, 1/1) elution separation obtains butyric acid-gibberellin haptens product
0.17g, yield 74%.
2, the preparation of immunogene
Gibberellin haptens 17mg is taken, adds n,N-Dimethylformamide (DMF) 1mL to dissolve, adds carbodiimides (EDC)
9.4mg, n-hydroxysuccinimide (NHS) 7.3mg, piping and druming mix, and react at room temperature 4h, obtain haptens activating solution A liquid；Take ox
Seralbumin (BSA) 50mg, adds 0.05mol/L PB buffer solution, obtains B liquid；A drop is added in B liquid, 2h is reacted,
0.02mol/L PBS buffer solution dialysis purification 3 days changes liquid 3 times daily, obtains gibberellin haptens-BSA conjugate, as exempt from
Epidemic focus, packing, -20 DEG C of preservations.
3, the preparation of coating antigen
Gibberellin haptens 11mg is taken, adds DMF 1mL to dissolve, adds ethylenediamine 0.2mL, is cooled to 0~5 DEG C, chloromethane is added dropwise
Sour 71 μ L of isobutyl ester, the reaction was continued 4h, obtain haptens activating solution A liquid；Ovalbumin (OVA) 50mg is taken, 0.05mol/LPB is added
Buffer solution obtains B liquid；A drop is added in B liquid, reaction 2h, 0.02mol/L PBS buffer solution dialysis purification 3 days, often
It is changed liquid 3 times, obtains red mould haptens element-OVA conjugate, as coating antigen, packing, -20 DEG C of preservations.
4, the preparation of gibberellin monoclonal antibody
(1) animal immune
In the immunogene injection Balb/c Mice Body that step 2 is obtained, immunizing dose is 150 μ g/, its is made to generate anti-blood
Clearly.
(2) cell fusion and cloning
Immune Balb/c mouse boosting cell is taken, is merged in 8:1 (quantitative proportion) ratio with SP2/0 myeloma cell, is used
Indirect competitive ELISA measures cell supernatant, screens positive hole.Cloning is carried out to positive hole using limiting dilution assay, until
Obtain the hybridoma cell strain of stably excreting monoclonal antibody.
(3) cell cryopreservation and recovery
Hybridoma is made 1 × 10 with frozen stock solution6The cell suspension of a/mL, saves for a long time in liquid nitrogen.When recovery
Cryopreservation tube is taken out, 37 DEG C of water-bath middling speeds is immediately placed in and melts, after centrifugation removal frozen stock solution, moves into culture culture in glassware.
(4) preparation and purification of monoclonal antibody
Increment cultivation: hybridoma is placed in cell culture medium, is cultivated under the conditions of 37 DEG C, with octanoic acid-
Saturated ammonium sulfate method purifies obtained culture solution, obtains monoclonal antibody, -20 DEG C of preservations.
The cell culture medium is that calf serum and sodium bicarbonate are added into RPMI1640 culture medium, and calf serum is made to exist
Final concentration of 20% (mass fraction) in cell culture medium, make sodium bicarbonate in cell culture medium final concentration of 0.2%
(mass fraction)；The pH of the cell culture medium is 7.4.
5, the preparation of sheep anti mouse antiantibody
Using sheep as immune animal, pathogen-free domestic sheep is immunized using source of mouse antibody as immunogene, it is anti-to obtain sheep anti mouse
Antibody.
6, gibberellin monoclonal antibody-colloid gold label object preparation
(1) preparation of colloidal gold
1% gold chloride is diluted to 0.01% (mass fraction) with double distilled deionized water, 100mL is taken to be placed in conical flask,
It is heated to boiling with thermostatic electromagnetic blender, in continuous high temperature, is persistently added with stirring 1% trisodium citrate of 2.5mL, continue
Solution is at the uniform velocity heated with stirring in stopping when bright red, is restored to original volume, 4 DEG C of guarantors with deionized water after being cooled to room temperature
It deposits.The colloidal gold appearance prepared is pure, it is bright, without precipitating and floating material, observing color in the sunlight is claret.
(2) gibberellin monoclonal antibody-colloid gold label object preparation
Under magnetic stirring, molten by every milliliter of colloidal gold with the pH value of 0.2mol/L solution of potassium carbonate tune colloidal gold to 7.2
Above-mentioned gibberellin monoclonal antibody is added into colloidal gold solution for the standard that 20~50 μ g antibody are added in liquid, continues to stir and evenly mix
30min；10%BSA is added after standing 10min, makes its final concentration of 1% in colloidal gold solution, stands 10min.
12000r/min, 4 DEG C of centrifugation 40min abandon supernatant, and precipitating is washed twice with redissolution buffer, are initial colloid gold with volume
The redissolution buffer of volume 1/10 will precipitating be resuspended, set 4 DEG C it is spare.
Redissolve buffer: the mass fraction containing BSA be 0.1%~0.3%, mass fraction 0.05% of Tween-80~
0.2%, the 0.02mol/L phosphate buffer of pH7.2.
7, the preparation of conjugate release pad
By conjugate release pad be soaked in containing 0.5%BSA, pH 7.2,0.5mol/L phosphate buffer in, uniformly soak
Wet 1h, 37 DEG C of baking 3h are spare.It is with Isoflow spray film instrument that the gibberellin prepared monoclonal antibody-colloid gold label object is uniform
It is sprayed in conjugate release pad, every 1cm conjugate release pad sprays 0.01mL gibberellin monoclonal antibody-colloid gold label object
Afterwards, it is taken out after being placed in 37 DEG C of environment (humidity < 20%) 60min, is placed in dry environment (humidity < 20%) and saves backup.
8, the preparation of sample absorption pad
By sample absorption pad be placed in containing 0.5% bovine serum albumin(BSA), pH 7.2,0.1mol/L phosphate buffer in
2h is impregnated, drying 2h is spare at 37 DEG C.
9, the preparation of reaction film
Detection line will be constituted in gibberellin haptens-ovalbumin conjugate coating to reaction film, by sheep anti mouse antiantibody
It is coated on reaction film and constitutes nature controlling line.
Coating process: being diluted to 1mg/mL for gibberellin haptens-ovalbumin conjugate with phosphate buffer, uses
Isoflow point film instrument is coated in the detection line (T line) on nitrocellulose filter, and package amount is 1.0 μ L/cm；With
Sheep anti mouse antiantibody is diluted to 200 μ g/mL by the phosphate buffer of 0.01mol/L, pH7.4, with Isoflow point film instrument by its
The nature controlling line (C line) being coated on nitrocellulose filter, package amount are 1.0 μ L/cm.The reaction film being coated with is placed in 37 DEG C of items
Dry 2h, spare under part.
10, the assembling of test strips
Test strips cross-section structure shown in 2 with reference to the accompanying drawings, by sample absorption pad (1), conjugate release pad (2), reaction film
(3), water absorption pad (4) is successively pasted onto order on PVC bottom plate (7)；Conjugate release pad has 1/3 region by sample from starting point
Absorption pad covering, the end of conjugate release pad are connected with the beginning of reaction film, the end of reaction film and the beginning phase of water absorption pad
Even, the beginning of sample absorption pad is aligned with the beginning of PVC bottom plate, and the end of water absorption pad is aligned with the end of PVC bottom plate；It is described anti-
Answering on film has detection line (5) and nature controlling line (6), and detection line (T line) and nature controlling line (C line) are to hang down with the appearance of the test strips
Straight strip tape；Detection line is located at the side close to the end of conjugate release pad；Nature controlling line is located remotely from conjugate release pad
End side；Test strips are cut into the small item of 3mm wide, in special plastics fabrication, 4~30 DEG C of environment with machine
Middle storage, validity period 12 months.
The detection of gibberellin in 2 bean sprouts of embodiment
1, the pre-treatment of sample
With homogenizer homogeneous bean sprouts sample (being sure not to be ground into powder)；2.0g ± 0.05g sample is weighed to 10mL polyphenyl
In ethylene centrifuge tube, 3mL phosphate buffer is added, is mixed with vortex mixed instrument whirling motion 1min；Room temperature (20~25 DEG C)
4000r/min is centrifuged 5min, takes supernatant liquor as sample to be tested liquid.
2, it is detected with test strips
80 μ L of sample to be tested liquid is drawn with micropipettor vertically to drip in well；Liquid flowing starts timing, reaction
8min determines result.
3, analysis detection result
Negative (-): the colour developing of T line is deeper than the colour developing of C line or consistent with the colour developing of C line, indicates that gibberellin concentration is lower than inspection in sample
Limit is surveyed, such as Fig. 3 a, 3b.
Positive (+): the colour developing of T line is more shallow than the colour developing of C line or T line does not develop the color, and indicates that gibberellin concentration is equal to or higher than in sample
Detection limit, such as Fig. 3 c, 3d.
It is invalid: not occur C line, show the deterioration failure of incorrect operating process or test strips, such as Fig. 3 e, 3f.
3 sample detection example of embodiment
1, detection limit test
Blank bean sprouts sample is taken, adds gibberellin respectively wherein to final concentration of 50 μ g/kg, 100 μ g/kg, 200 μ g/
Kg takes test strips to be detected, and each sample is repeated three times.
When detecting bean sprouts sample with test strips, when wherein without gibberellin and its to add concentration be 50 μ g/kg when, in test strips
It shows that the colour developing of T line develops the color deep or develops the color unanimously with C line than C line, is negative；When wherein gibberellin addition concentration is 100 μ g/
When kg, 200 μ g/kg, shows that the colour developing of T line is more shallow than the colour developing of C line or T line does not develop the color in test strips, be positive, show this test strips
100 μ g/kg are limited to the detection of gibberellin in bean sprouts.
2, false positive rate, false negative rate test
Take blank bean sprouts sample and addition gibberellin to each 20 parts of positive bean sprouts sample of final concentration of 100 μ g/kg, with 3
The test strips of a batch production are detected respectively, calculate its yin and yang attribute rate.It the results are shown in Table 1.
Table 1 detects bean sprouts sample results
The result shows that: when detecting positive bean sprouts sample with the test strips of 3 batch productions, as a result it is all positive, it is known that sun
Property coincidence rate be 100%, false negative rate 0；When detecting 20 parts of negative bean sprouts samples, as a result it is all negative, it is known that feminine gender meets
Rate is 100%, false positive rate 0.Illustrate that the test strips of detection gibberellin of the invention can be residual to gibberellin in the sample of bean sprouts
It stays and is used for quickly detecting.
3, specific test
With this test strips detect 1000 μ g/kg 4-chlorophenoxyacetic acids, 2,4,5 trichlorophenoxyacetic acid, 6-benzyladenine,
When the other plants growth regulator such as methyl α-naphthyl acetate, heteroauxin, indolebutyric acid, paclobutrazol, Thidiazuron, T is shown in test strips
Line colour developing develops the color deep or develops the color unanimously with C line than C line, is negative, illustrates this test strips to these drug no cross reactions.
Claims (5)
1. a kind of test strips for detecting gibberellin, including sample absorption pad, conjugate release pad, reaction film, water absorption pad and bottom plate,
There is the detection line for being coated with gibberellin hapten-carrier protein conjugate on the reaction film and be coated with sheep anti mouse antiantibody
Nature controlling line, be coated with gibberellin monoclonal antibody-colloid gold label object in the conjugate release pad；The gibberellin Dan Ke
Grand antibody is prepared using gibberellin hapten-carrier protein conjugate as immunogene；The gibberellin hapten-carrier
Protein conjugate is obtained by gibberellin haptens with carrier protein couplet, and the carrier protein is bovine serum albumin(BSA), egg white egg
White, hemocyanin, thyroprotein or human serum albumins, it is characterised in that the gibberellin haptens is aoxidized by gibberellin
It reacts to obtain with butyric acid hydroxyl oxime again afterwards, molecular structural formula are as follows:
2. test strips as described in claim 1, it is characterised in that the sample absorption pad, conjugate release pad, reaction film, suction
Water cushion is successively pasted on bottom plate.
3. such as the described in any item test strips of claim 1-2, it is characterised in that the conjugate release pad 1/3~1/2 is coating
It is placed under sample absorption pad.
4. a kind of method for preparing any one of claim 1-3 test strips comprising step:
1) preparation is coated with gibberellin monoclonal antibody-colloid gold label object conjugate release pad；
2) preparation has the detection line for being coated with gibberellin hapten-carrier protein conjugate and is coated with sheep anti mouse antiantibody
The reaction film of nature controlling line；
And 2) 3) 1) the conjugate release pad, reaction film and sample absorption pad, water absorption pad and the bottom plate that prepare are assembled into test paper
Item.
5. the remaining method of gibberellin in a kind of sample of detection bean sprouts comprising step:
1) sample pre-treatments；
2) it is detected with the described in any item test strips of claim 1-3；
3) analysis detection result.
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| CN111289752A (en) * | 2020-03-05 | 2020-06-16 | 北京勤邦生物技术有限公司 | Test strip and method for detecting dicofol |
| CN113030463A (en) * | 2021-02-04 | 2021-06-25 | 北京勤邦生物技术有限公司 | Test strip for detecting protein A and other impurities in vaccine and application thereof |
| CN114249705A (en) * | 2021-12-24 | 2022-03-29 | 苏州快捷康生物技术有限公司 | Preparation and application of gibberellin hapten and gibberellin antigen |
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| CN111289752A (en) * | 2020-03-05 | 2020-06-16 | 北京勤邦生物技术有限公司 | Test strip and method for detecting dicofol |
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| CN114317452A (en) * | 2022-01-25 | 2022-04-12 | 无锡迪腾敏生物科技有限公司 | Naphthylacetic acid monoclonal antibody, hybridoma cell strain and application |
| CN114317452B (en) * | 2022-01-25 | 2023-11-28 | 无锡迪腾敏生物科技有限公司 | Naphthylacetic acid monoclonal antibody, hybridoma cell strain and application |
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