CN109234172A - 一种防治大叶黄杨白粉病的复合微生物菌剂 - Google Patents

一种防治大叶黄杨白粉病的复合微生物菌剂 Download PDF

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CN109234172A
CN109234172A CN201811217951.9A CN201811217951A CN109234172A CN 109234172 A CN109234172 A CN 109234172A CN 201811217951 A CN201811217951 A CN 201811217951A CN 109234172 A CN109234172 A CN 109234172A
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powdery mildew
bacterium solution
euonymus japonicus
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王罡
张亚楠
季静
孙培
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Tianjin University
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Abstract

本发明公开了一种防治大叶黄杨白粉病的复合微生物菌剂，包括如下步骤：(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于25～28℃，150～180rpm的摇床中震荡培养22‑26h，得到菌液A；(2)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于30～35℃，150～180rpm的摇床中震荡培养22‑26h，得到菌液B；(3)按质量比，将7～8份菌液A、2～3份菌液B混合，得到混合菌液，加双蒸水至100份，得到防治大叶黄杨白粉病的复合微生物菌剂。本发明的优点：本发明的复合微生物菌剂成本低，简单快捷，能有效防治大叶黄杨白粉病，见效快，治病效果好。

Description

一种防治大叶黄杨白粉病的复合微生物菌剂
技术领域
本发明涉及植物病害防治领域，特别涉及一种防治大叶黄杨白粉病的复合微生物菌剂。
技术背景
大叶黄杨(Euonymus japonicus Thunb)又称冬青卫矛、正木，属卫矛科，为温带亚热带树木，在我国广泛栽培，是园林绿化中常见的绿篱植物和造型材料，具有较大的经济和社会效益。据报道，大叶黄杨病害发生较为普遍，其中大叶黄杨白粉病较严重。大叶黄杨白粉病是由大叶黄杨白粉病菌(Erysipheeuonymi-japonici)侵染引起的，该病自幼苗到生长期均可发生，主要危害大叶黄杨叶片、茎，而嫩叶和新梢最易受白粉病菌侵染。发病初期在叶面或叶背及嫩梢表面散生白色小圆点，边缘呈放射状，而后病斑逐渐扩大，叶片及叶背和嫩梢布满白色粉状霉层，叶片背面比正面多。之后霉层覆盖全叶，使叶卷曲，枝梢扭曲变形，最后叶片枯死脱落，严重影响了大叶黄杨的观赏价值，使其在园林绿化中的应用受到限制。在生产中发现，有些地区由于长期使用单一的农药进行防治，某些白粉病菌菌株已出现耐药性。
目前对大叶黄杨白粉病的防治应急方法主要是通过喷施化学药剂来杀死病原菌，而杀菌化学试剂的滥用极易造成病原菌产生抗药性。因此，采用生物防治的方法对大叶黄杨白粉病的防治势在必行。生物防治的方法是通过生防菌的生防功能实现的，生防菌是一类具有生物防治效果的菌群，其特点在于绿色环保，对植物无毒副作用，能更好的抑制病原菌甚至其拮抗作用能杀死病原菌，通过喷施原始菌种来防治大叶黄杨白粉病具有良好的效果。
目前，已知的治疗大叶黄杨白粉病的生物防治方法是喷洒枯草芽孢杆菌发酵液，且发酵液随着时间的推移药效降低，没有直接喷施生防菌剂药效持久。但喷施单一生防菌剂防治效果没有复合菌剂药效好，且复合菌剂的配比对防治效果具有很大的影响，因此选择适当配比的复合菌剂防治大叶黄杨白粉病势在必行。
发明内容
本发明的目的是克服现有技术的不足，提供一种防治大叶黄杨白粉病的复合微生物菌剂。
本发明的技术方案概述如下：
一种防治大叶黄杨白粉病的复合微生物菌剂，包括如下步骤：
(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于25～28℃，150～180rpm的摇床中震荡培养22-26h，得到菌液A；
(2)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于30～35℃，150～180rpm的摇床中震荡培养22-26h，得到菌液B；
(3)按质量比，将7～8份菌液A、2～3份菌液B混合，得到混合菌液，加双蒸水至100份，得到防治大叶黄杨白粉病的复合微生物菌剂。
本发明的优点：本发明的复合微生物菌剂成本低，简单快捷，能有效防治大叶黄杨白粉病，见效快，治病效果好。
附图说明
图1喷施对比例和实施例中的复合物生物菌剂后7天、14天、21天、28天新叶的染病面积在50％以下的叶片数量。
图2喷施对比例和实施例中的复合物生物菌剂后7天、14天、21天、28天老叶的染病面积在50％以下的叶片数量。
具体实施方式
PDA液体培养基购自南通凯恒生物科技发展有限公司
LB液体培养基配方：酵母粉5g,蛋白胨10g,NaCl 10g,ddH2O 1L.
下面通过具体实施例对本发明作进一步的说明。
菌种：淡紫拟青霉菌(Paecilomyces lilacinus(Thom)Samson)于2017年6月购自北纳生物，www.bnbio.com,中国，菌种保藏编号为BNCC119092。
胶质芽孢杆菌(Bacillus mucilaginosus Krassilnikov)于2017年6月购自北纳生物，www.bnbio.com,中国，菌种保藏编号为BNCC335819
实施例1
一种防治大叶黄杨白粉病的复合微生物菌剂，包括如下步骤：
(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于25℃，150rpm的摇床中震荡培养26h，得到菌液A；
(2)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于30℃，150rpm的摇床中震荡培养26h，得到菌液B；
(3)将7ml菌液A、3ml菌液B混合，得到混合菌液，加双蒸水至100ml，得到防治大叶黄杨白粉病的复合微生物菌剂。
实施例2
一种防治大叶黄杨白粉病的复合微生物菌剂，包括如下步骤：
(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于28℃，180rpm的摇床中震荡培养22h，得到菌液A；
(2)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于35℃，180rpm的摇床中震荡培养22h，得到菌液B；
(3)将8ml菌液A、2ml菌液B混合，得到混合菌液，加双蒸水至100ml，得到防治大叶黄杨白粉病的复合微生物菌剂。
对比例1
(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于25℃，150rpm的摇床中震荡培养26h，得到菌液A；
(2)取10ml菌液A,加双蒸水至100ml。
对比例2
(1)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于30℃，150rpm的摇床中震荡培养26h，得到菌液B；
(2)取10ml菌液B,加双蒸水至100ml。
实验：
选取大叶黄杨染白粉病的叶片面积在90％及以上的老叶、新叶各100片(还在树上生长着的)，作为实施例和对比例中的喷施对象。将各实施例和对比例中的菌剂分别装于500ml的喷壶中，对喷施对象的正反两面分别喷3次，菌液体积共3-4ml。
在喷施菌剂后叶片染病面积减小且染病面积小于50％的叶片作为有效果的叶片进行统计。
分别统计喷施后第7天、第14天、第21天、第28天新叶、老叶有效果的叶片数量。见图1和图2。
试验表明，对于大叶黄杨白粉病的防治：实施例1和实施例2的效果优于对比例。

Claims (1)

1.一种防治大叶黄杨白粉病的复合微生物菌剂，其特征是包括如下步骤：
(1)将淡紫拟青霉菌接种于100ml的PDA液体培养基中，于25～28℃，150～180rpm的摇床中震荡培养22-26h，得到菌液A；
(2)将胶质芽孢杆菌接种于100ml的LB液体培养基中，于30～35℃，150～180rpm的摇床中震荡培养22-26h，得到菌液B；
(3)按质量比，将7～8份菌液A、2～3份菌液B混合，得到混合菌液，加双蒸水至100份，得到防治大叶黄杨白粉病的复合微生物菌剂。
CN201811217951.9A 2018-10-18 2018-10-18 一种防治大叶黄杨白粉病的复合微生物菌剂 Pending CN109234172A (zh)

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