CN109234005A - A kind of extracting process of squids tissue fat - Google Patents
A kind of extracting process of squids tissue fat Download PDFInfo
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- CN109234005A CN109234005A CN201811225450.5A CN201811225450A CN109234005A CN 109234005 A CN109234005 A CN 109234005A CN 201811225450 A CN201811225450 A CN 201811225450A CN 109234005 A CN109234005 A CN 109234005A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of extracting process of squids tissue fat, based on Soxhlet leaching device (fatty leaching instrument), the fatty material for carrying out the tissues such as squids muscle, glandula digestive effectively, quickly, accurately extracts, reduce the experimental cost and time cost of traditional soxhlet type, the precision for improving extracting fat extracting reduces issuable pollution condition during repeating extractive distillation due to biological tissue's fat to the maximum extent and occurs.The present invention solves traditional soxhlet extraction methods to the shortage of the extracting fat method for extracting of the tissues such as squids muscle, glandula digestive, standardizes extractive distillation method, the step to this kind of biological tissue's fat；Solve traditional soxhlet extraction methods because there are extraction of lipid it is unclean due to carry out the extractive distillations of the tissue fats such as squids muscle, glandula digestive over and over again, cause experimental cost and time cost to be increased and be easy to cause the contaminated problem of biological tissue.
Description
Technical field
The invention belongs to field of biotechnology, are science, effectively extract the tissue such as squids muscle, glandula digestive, sexual gland
Fat, to be applied to carry out the biomarkers such as stable isotope, fatty acid in the fields such as marine ecology, breeding ecology
Measurement.
Background technique
Siphonopods is important marine economic animal, becomes the following fishery for most having potentiality to be exploited by FAO (Food and Agriculture Organization of the United Nation)
One of resource category.Cephalopods In The World fishery production increases year by year, and the status in world's sea fishery is more and more important.Its
In, squids are that the important and main fished species of Cephalopods In The World fishery and the important of China's distant water squid jigging fisheries catch
Fish out type.It is shown according to the latest data of FAO (Food and Agriculture Organization of the United Nation), nearly ten years the squids total catch of world commerce fishing
About 3,000,000 tons/year.Meanwhile these types occupy important ecological status in marine ecosystems, are in marine food
Middle-of-chain position plays important " bio-pump " effect；And in different developmental stages, these types play transmitting sea
The important instrumentality of foreign bio-energy.
When carrying out squids bioecology, breeding ecology research, the biomarkers such as stable isotope, fatty acid
Measurement analysis is an important research contents.These biomarkers measure analytical technology it is verified that raw for research biology
Effective and scientific experimental technique method of state structure, Feeding Ecology trophic level, reproduction Energy input etc., and extensively
Ground is applied to the research in the fields such as marine organisms Feeding Ecology, marine organisms breeding ecology, can taking the photograph with tracer marine organisms
Food biological source, the ecoalimental grade of predator, bion are ingested the accumulation and its investment distribution etc. of surplus energy.It uses
Stabilization isotron, fatty acid gaseous mass analyzer, with it is simple, easy, safely and fast measure biological sample carbon nitrogen stablize
Isotope, fatty acid composition etc..However, the lipid of biological tissue can cause the significant changes of stable isotope determination result, drop
Low-carbon Stable isotope ratio.Meanwhile when carrying out biological tissue's fatty acid composition, demand effectively extracts the lipid of tissue.Therefore,
Before carrying out the measurement analysis of these biomarkers, it is required to the fat of extracting biological tissue.
Currently, the method for extracting biological tissue's fat is mainly soxhlet extraction methods.Traditional soxhlet extraction methods are mainly applied
In plant soybean, it is applied to other biologies, the method for especially biological tissues' extracting fat such as ocean squids extracting rarely has rule
Model.Meanwhile experimental cost required for traditional soxhlet extraction methods and time cost are higher, experimental procedure may be because of operator not
It has differences together, the clean required extraction times of tissue fat extraction are also because there are great differences for different biological tissues.In addition,
When extracting fat extracting is not clean repeats extracting experiment, also tend to be easy to cause the experimental pollution of biological tissue general
Rate influences final biomarker measurement result.In this way, nonstandard biological tissue's extracting fat method for extracting is not only made
It is reduced at carbon stable isotope measurement result precision, also will increase biological tissue's fatty acid composition composition and its assay not
Certainty.
Therefore, it before carrying out the measurements such as squid class loading carbon and nitrogen stable isotope, fatty acid composition analysis, needs fast
Victory, accurately, effectively extractive distillation fat, to improve carbon stable isotope measurement result precision and fatty acid composition composition
And its measurement result of content.
Summary of the invention
Technical problem solved by the invention is to provide a kind of extracting process of squids tissue fat, be soaked based on Soxhlet
It mentioning device (fatty leaching instrument), the fatty material for carrying out the tissues such as squids muscle, glandula digestive effectively, quickly, accurately extracts,
Reduce the experimental cost and time cost of traditional soxhlet type, improve the precision of extracting fat extracting, reduce to the maximum extent by
Issuable pollution condition occurs during biological tissue's fat repeats extractive distillation, to solve in above-mentioned background technique
Disadvantage.
Technical problem solved by the invention is realized using following technical scheme: a kind of extraction of squids tissue fat
Method, comprising the following steps:
(1), the fresh sample of squids trunk musculature is acquired, digestion glandular tissue is entirely won；
(2), the fresh sample of musculature, digestion glandular tissue hold preservation respectively, and subzero 80 DEG C of freezen protectives 24 hours；
(3), the fresh sample of musculature, digestion glandular tissue are placed in freeze drier after freezen protective, are freeze-dried 48 hours；
(4), the musculature after being freeze-dried weighs drying sample weight, grinds, and is placed in degreasing filter cylinder；
(5), the digestion glandular tissue after being freeze-dried weighs drying sample weight, is placed in degreasing filter cylinder；
(6), the weight for extracting beaker that Soxhlet leaching device is clean, dry is weighed；
(7), the degreasing filter cylinder equipped with musculature, the dry sample of digestion glandular tissue is respectively put by six one group
The Soxhlet leaching device of preheating is that tissue fat extracts Extraction solvent using chloroform-methanol mixed solvent；
(8), the extraction time that musculature extracting fat extracts is as follows: boiling time about 30 minutes, the elution time was about
35 minutes, recovery time about 15 minutes, drying time about 30 minutes；
(9), the extraction time that the extraction of glandula digestive tissue fat is extracted is as follows: boiling time about 45 minutes, eluting the time
About 50 minutes, recovery time about 15 minutes, drying time about 30 minutes；
(10), the weight of extraction cup and residue after weighing drying；
(11), the fat content for calculating muscle, digesting glandular tissue, calculation formula are as follows:
In formula, LC is the fat content of tissue, unit %；dryW1For the sample powder weight for being placed in degreasing filter cylinder, unit g；
dryW2For the weight of clean, dry extraction beaker, unit g；dryW3Extraction beaker for fat extraction extraction and after drying
And residue weight, unit g.
Further, in step (1), squids trunk musculature fresh sample about 10.00g is acquired, in step (4), is claimed
It takes the comminuted sample merging degreasing filter cylinder that 0.2g is dry, in step (5), weighs 0.1g sample merging degreasing filter cylinder；The step
(4), the precision that weighs in (5), (6) and (10) is accurate to ± 0.001g.
Further, it is smash using mortar grinder and permits entire glandula digestive, then be placed in degreasing filter cylinder.
Further, in step (7), preheating temperature is set as 110 DEG C.
Further, in step (7), the ratio of chloroform and methanol is 2:1..
The present invention realize squids muscle, the tissue fats such as glandula digestive quickly, accurately, effectively extractive distillation,
To reach two purposes:
Traditional soxhlet extraction methods are solved to the shortage of the extracting fat method for extracting of the tissues such as squids muscle, glandula digestive,
Extractive distillation method, step of the creativeness specification to this kind of biological tissue's fat；
Solve traditional soxhlet extraction methods because there are extraction of lipid it is unclean due to carry out squids muscle, digestion over and over again
The extractive distillation of the tissue fats such as gland causes experimental cost and time cost to be increased and be easy to cause biological tissue dirty
The problem of dye.
Detailed description of the invention
Fig. 1 is jumbo flying squid trunk of the invention, the fat content schematic diagram for digesting glandular tissue.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Conjunction is specifically illustrating, and the present invention is further explained.
A kind of extracting process of squids tissue fat carries out squids flesh based on Soxhlet leaching device (fatty leaching instrument)
The fatty materials of the tissues such as meat, glandula digestive effectively, quickly, accurately extractive distillation, comprising the following steps:
1, the fresh sample ≈ 10.00g of squids trunk musculature is acquired；Digestion glandular tissue is entirely won；
2, the fresh sample of musculature, glandula digestive hold preservation respectively, and subzero 80 DEG C of freezen protectives 24 hours；
3, the fresh sample of musculature, glandula digestive are placed in freeze drier after freezen protective, are freeze-dried 48 hours；
4, the musculature after being freeze-dried weighs drying sample weight, grinds；Weigh the dry crushing sample of 0.2g
This merging degreasing filter cylinder, is accurate to ± 0.001g；
5, the digestion glandular tissue after being freeze-dried, weighs drying sample weight.Glandula digestive fat content is higher, uses mortar
Grinding, which is smash, permits entire glandula digestive；0.1g sample merging degreasing filter cylinder is weighed, ± 0.001g is accurate to；
6, the weight for extracting beaker that Soxhlet leaching device is clean, dry is weighed, ± 0.001g is accurate to；
7, the Soxhlet having been warmed up is respectively put by six one group equipped with the dry degreasing filter cylinder of muscle, digestion glandular tissue
Leaching device (temperature is set as 110 DEG C) is that tissue fat extracts Extraction solvent using chloroform-methanol (2:1) mixed solvent.
8, the extraction time that musculature extracting fat extracts is as follows: boiling time ≈ 30 minutes, elution time ≈ 35 divided
Clock, recovery time ≈ 15 minutes, drying time ≈ 30 minutes；
9, the extraction time that the extraction of glandula digestive tissue fat is extracted is as follows: boiling time ≈ 45 minutes, eluting time ≈ 50
Minute, recovery time ≈ 15 minutes, drying time ≈ 30 minutes；
10, the weight of extraction cup and residue after weighing drying, is accurate to ± 0.001g；
11, the fat content for calculating muscle, digesting glandular tissue, calculation formula are as follows:
In formula, LC is the fat content of tissue, unit %；DryW1 is the sample powder weight for being placed in degreasing filter cylinder, unit g；
DryW2 is the weight of clean, dry extraction beaker, unit g；Extraction after dryW3 is fat extraction extraction and dries is burnt
Cup and residue weight, unit g.
12, according to above-mentioned steps and formula, trunk and glandula digestive to tail jumbo flying squid carry out extracting fat.As a result such as Fig. 1
Shown, the fat content of trunk tissue is 15.82%~21.38%, and average value is 19.46 ± 1.04%；The fat of glandula digestive
Content is 40.14%~65.98%, and average value is 51.65 ± 9.95%.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (5)
1. a kind of extracting process of squids tissue fat, comprising the following steps:
(1), the fresh sample of squids trunk musculature is acquired, digestion glandular tissue is entirely won；
(2), the fresh sample of musculature, digestion glandular tissue hold preservation respectively, and subzero 80 DEG C of freezen protectives 24 hours；
(3), the fresh sample of musculature, digestion glandular tissue are placed in freeze drier after freezen protective, are freeze-dried 48 hours；
(4), the musculature after being freeze-dried weighs drying sample weight, grinds, and is placed in degreasing filter cylinder；
(5), the digestion glandular tissue after being freeze-dried weighs drying sample weight, is placed in degreasing filter cylinder；
(6), the weight for extracting beaker that Soxhlet leaching device is clean, dry is weighed；
(7), the degreasing filter cylinder equipped with musculature, the dry sample of digestion glandular tissue is respectively put into and has been warmed up by six one group
Soxhlet leaching device, using chloroform-methanol mixed solvent be tissue fat extract Extraction solvent；
(8), the extraction time that musculature extracting fat extracts is as follows: boiling time about 30 minutes, eluting about 35 points of the time
Clock, recovery time about 15 minutes, drying time about 30 minutes；
(9), the extraction time that the extraction of glandula digestive tissue fat is extracted is as follows: boiling time about 45 minutes, the elution time was about
50 minutes, recovery time about 15 minutes, drying time about 30 minutes；
(10), the weight of extraction cup and residue after weighing drying；
(11), the fat content for calculating muscle, digesting glandular tissue, calculation formula are as follows:
In formula, LC is the fat content of tissue, unit %；dryW1For the sample powder weight for being placed in degreasing filter cylinder, unit g；dryW2
For the weight of clean, dry extraction beaker, unit g；dryW3Extraction beaker for fat extraction extraction and after drying and residual
Stay object weight, unit g.
2. a kind of extracting process of squids tissue fat as described in claim 1, it is characterised in that: in step (1), acquisition
Squids trunk musculature fresh sample about 10.00g in step (4), weighs the dry comminuted sample merging degreasing filter of 0.2g
Tin, in step (5), weigh 0.1g sample merging degreasing filter cylinder；Precision essence is weighed in the step (4), (5), (6) and (10)
Really arrive ± 0.001g.
3. a kind of extracting process of squids tissue fat as described in claim 1, it is characterised in that: smash using mortar grinder
Permit entire glandula digestive, then is placed in degreasing filter cylinder.
4. a kind of extracting process of squids tissue fat as described in claim 1, it is characterised in that: in the step (7),
Preheating temperature is set as 110 DEG C.
5. a kind of extracting process of squids tissue fat as described in claim 1, it is characterised in that: in the step (7),
The ratio of chloroform and methanol is 2:1..
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| CN201811225450.5A CN109234005A (en) | 2018-10-20 | 2018-10-20 | A kind of extracting process of squids tissue fat |
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| CN201811225450.5A CN109234005A (en) | 2018-10-20 | 2018-10-20 | A kind of extracting process of squids tissue fat |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114923804A (en) * | 2022-03-09 | 2022-08-19 | 陇南市祥宇油橄榄开发有限责任公司 | Method for determining fat content in olive fruit |
| CN115308262A (en) * | 2022-08-09 | 2022-11-08 | 上海海洋大学 | Method for measuring fat energy contribution degree of gonad tissue of soft fishes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356688A (en) * | 2017-07-07 | 2017-11-17 | 上海海洋大学 | A kind of method for distinguishing multiple geographical population jumbo flying squids using muscle essential fatty acid composition |
| CN107603744A (en) * | 2017-10-23 | 2018-01-19 | 无锡超科食品有限公司 | A kind of fatty method in extraction powdered oil |
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2018
- 2018-10-20 CN CN201811225450.5A patent/CN109234005A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356688A (en) * | 2017-07-07 | 2017-11-17 | 上海海洋大学 | A kind of method for distinguishing multiple geographical population jumbo flying squids using muscle essential fatty acid composition |
| CN107603744A (en) * | 2017-10-23 | 2018-01-19 | 无锡超科食品有限公司 | A kind of fatty method in extraction powdered oil |
Non-Patent Citations (1)
| Title |
|---|
| 贡艺等: "脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响", 《应用生态学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114923804A (en) * | 2022-03-09 | 2022-08-19 | 陇南市祥宇油橄榄开发有限责任公司 | Method for determining fat content in olive fruit |
| CN115308262A (en) * | 2022-08-09 | 2022-11-08 | 上海海洋大学 | Method for measuring fat energy contribution degree of gonad tissue of soft fishes |
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Application publication date: 20190118 |