CN109221301B - Complexing agent for inhibiting growth and competition of Eupatorium adenophorum and preparation method thereof - Google Patents

Complexing agent for inhibiting growth and competition of Eupatorium adenophorum and preparation method thereof Download PDF

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CN109221301B
CN109221301B CN201811261445.XA CN201811261445A CN109221301B CN 109221301 B CN109221301 B CN 109221301B CN 201811261445 A CN201811261445 A CN 201811261445A CN 109221301 B CN109221301 B CN 109221301B
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soil
fungicide
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eupatorium adenophorum
active carbon
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CN109221301A (en
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李象钦
唐赛春
韦春强
潘玉梅
吕仕洪
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Guangxi Institute of Botany of CAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass

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  • Agronomy & Crop Science (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)
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Abstract

The invention discloses a complexing agent for inhibiting growth and competition of Eupatorium adenophorum spreng and a preparation method thereof. In the field where the eupatorium adenophorum is invaded, the compound agent which is composed of the plant source growth inhibitor, the allelochemical adsorbent active carbon and the fungicide according to a certain dosage proportion is used for inhibiting the growth of the eupatorium adenophorum, weakening the influence of allelochemical and the influence of soil fungi, reducing the competitive power of the eupatorium adenophorum while inhibiting the growth of the eupatorium adenophorum, and improving the growth and the competition of local plants. The compound agent can obviously inhibit the germination and growth of the eupatorium adenophorum, reduces the competitive power of the eupatorium adenophorum to local plants, and has the advantages of high efficiency, sustainability, contribution to local species recovery, ecological safety and the like.

Description

Complexing agent for inhibiting growth and competition of Eupatorium adenophorum and preparation method thereof
Technical Field
The invention belongs to the field of biological control, and particularly relates to a complexing agent for inhibiting growth and competition of Eupatorium adenophorum spreng, and a preparation method and a use method thereof.
Background
Eupatorium adenophorum, commonly known as destructive grass, is a malignant weed with strong vitality, high reproduction rate and difficult cleaning, and is ranked the first in the first foreign invasive species in China. It inhibits the growth of surrounding plants, causes the soil fertility to be reduced, degrades the land, and brings great harm to the growth of crops and the cultivation of livestock. Related departments inhibit the spread of the eupatorium adenophorum by various means such as chemical control, biological control, processing and utilization and the like for years, but the effect is little.
Eupatorium adenophorum is a perennial herbaceous plant, native to Central America, and after being introduced into China from Burma in the last 40 th century, the Eupatorium adenophorum is continuously diffused and spread, occupies a large amount of abandoned cultivated land, returned to forest land, cultivated land, forest land and the like, and brings huge threat and damage to local agriculture and forestry. In some natural ecosystems, develop into dominant species, jeopardizing biodiversity and ecosystem architecture and function. The Eupatorium adenophorum can secrete allelochemicals, inhibit the growth of adjacent plants, change soil fungi, and improve competitiveness. The invasive plant is also toxic, and the livestock is easy to be poisoned by eating the invasive plant by mistake. Due to its great negative effects, Eupatorium adenophorum is listed as one of the most harmful invasive species in China. This species is also a malignant weed that is harmful to the united states, australia and many african and asian countries.
Many researches show that the eupatorium adenophorum has higher relative growth rate and larger biomass, and can improve the competitive capacity and successfully invade through the influence of allelopathy and fungi. Eupatorium adenophorum secretes allelochemicals, which inhibit seed germination and growth of many native plants, and moreover, the allelochemicals secreted therefrom can be present in soil for a long time. The eupatorium adenophorum can change fungi in soil of an invaded land, and is beneficial to the growth and the competitive action of the eupatorium adenophorum. In addition, the seeds of the eupatorium adenophorum can be kept in soil for a long time, and when the conditions are proper, the seeds germinate to generate new plants.
At present, many methods including chemical control, biological control and mechanical control are applied to the control of Eupatorium adenophorum. In contrast, alternative control is the best choice due to safety, environmental friendliness, economy, and sustainability. However, since Eupatorium adenophorum can secrete allelochemicals into the soil, these allelochemicals can remain in the soil for at least 2 years even after the Eupatorium adenophorum is removed; eupatorium adenophorum can produce a large number of seeds, and these seeds can form a long-lasting seed bank and will re-germinate and again compete with replacement plants. Therefore, simple removal of Eupatorium adenophorum can not ensure successful ecological restoration of the invaded land. This makes it necessary for replacement control not only to inhibit the growth of Eupatorium adenophorum, but also to reduce the competitive action of Eupatorium adenophorum on the replacement plants by weakening the allelopathic effect and the effect of soil fungi, so as to achieve the goal of controlling Eupatorium adenophorum and restoring ecology.
Some native plants are capable of inhibiting the germination and growth of invasive plants and can be developed as natural inhibitors. The activated carbon can adsorb secondary metabolites of plants. Fungicides can kill fungi and remove their effects. However, to date, there has been no study of reducing the growth of Eupatorium adenophorum using natural growth inhibitors and simultaneously reducing its competitive power by attenuating its allelopathic and fungal effects.
Disclosure of Invention
The invention aims to provide a complexing agent for inhibiting the growth and competition of Eupatorium adenophorum and improving the habitat of an invaded place and a preparation and use method thereof aiming at the defects of the prior art.
The purpose of the invention is realized by the following technical scheme:
a compound agent for inhibiting the growth and competition of Eupatorium adenophorum spreng, which comprises a plant source growth inhibitor, active carbon and fungicide:
the plant source growth inhibitor is as follows: tea strip leaf powder, folium Artemisiae Argyi powder or similar reed powder;
the powder is prepared by respectively taking leaves of tea strip wood, or aerial parts of mugwort and reed rhizome, drying, and pulverizing to 200 mesh;
the active carbon is as follows: the granular activated carbon AR is analytically pure;
the fungicide is: thiophanate-methyl.
In the complexing agent, the mixture ratio of the plant source growth inhibitor, the active carbon and the fungicide is as follows:
plant-derived growth inhibitor: 37.5g per kg soil;
activated carbon: 37.5-62.5g per kg soil;
fungicides: 0.15-0.47g per kg soil.
In the complexing agent, the optimal mixture ratio of the plant source growth inhibitor, the active carbon and the fungicide is as follows:
plant-derived growth inhibitor: 37.5g per kg soil;
activated carbon: 62.5g per kg soil;
fungicides: 0.28g per kg soil.
The invention firstly screens 3 plants: the above-ground parts of the tea strip wood leaves, the mugwort and the reed-like roots are dried and then crushed into 200 meshes to prepare powder, the water extracts of the three plants can reduce the germination rate of the eupatorium adenophorum, and the powder can inhibit the growth of the eupatorium adenophorum. Then, the formula of the complexing agent is optimized through an orthogonal experiment, namely, the powder of three plants, namely tea-strip wood, mugwort and reed-like, is mixed with active carbon (62.5 g, 47.0g and 37.5g and fungicide (0.47 g, 0.28g and 0.15g and fungicide) and fungicide (37.5 g and fungicide) according to different proportioning amounts of the soil respectively, so that the optimal formula capable of reducing the growth and competition of the eupatorium adenophorum is screened, namely the powder of tea-strip wood leaves is used for being 37.5g and the active carbon is used for being 62.5g and the fungicide is used for being 0.28 g.
The tea strip and wood leaf powder has no pollution to the environment, is a pure natural plant component, and can reduce seed germination and plant height, leaf number, total leaf area, relative growth rate, total biomass and competitive power of the eupatorium adenophorum.
Because the invasion of the eupatorium adenophorum can change soil fungi, the competitive capacity of the eupatorium adenophorum is improved, and after the fungicide is added, the action of the eupatorium adenophorum mediated soil fungi can be reduced, and the competitive capacity of the eupatorium adenophorum is influenced.
The invention relates to a preparation method of a complexing agent for inhibiting growth and competition of Eupatorium adenophorum, which comprises the steps of adding 37.5g of plant source growth inhibitor and 37.5-62.5g of active carbon into each kg of soil, uniformly mixing the plant source growth inhibitor and the active carbon, adding 0.15-0.47g of fungicide into each kg of soil after uniform mixing, and uniformly mixing to prepare the complexing agent.
At present, the growth and competition of the eupatorium adenophorum are inhibited, no complexing agent is used for people, only a single preparation of active carbon is used for improving soil, or a fungicide is used for killing soil fungi singly, so that the influence of the fungi is reduced. The compound agent can inhibit the growth of the eupatorium adenophorum, improve the soil to remove the allelopathy of the eupatorium adenophorum, kill soil fungi, reduce the competition of the eupatorium adenophorum, improve the growth and the competition of local plants and effectively restore the ecology of an invasive land.
The compound agent of the invention screens out the plant source growth inhibitor for inhibiting the eupatorium adenophorum, and the compound agent is prepared by the active carbon and the fungicide according to different dosages, thereby not only inhibiting the growth and competition of the eupatorium adenophorum, but also improving the soil of the invaded land. The method avoids the pollution of chemical control to the ecological environment and the residue of eupatorium adenophorum allelopathic substances in soil, improves the competitive power of local plants, promotes the recovery of the local plants, and plays an important role in the control of the eupatorium adenophorum and the ecological recovery of an invasive land.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited thereto.
Example 1:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of tea strip wood leaves, 62.5g/kg of soil of active carbon and 0.47g/kg of soil of fungicide.
Example 2:
the compounding agent comprises the following components in percentage by weight: the mugwort powder is 37.5g/kg soil, the active carbon is 62.5g/kg soil, and the fungicide is 0.28g/kg soil.
Example 3:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of the reed-like powder, 62.5g/kg of soil of the active carbon and 0.15g/kg of soil of the fungicide.
Example 4:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of tea strip wood leaves, 47.0g/kg of soil of active carbon and 0.28g/kg of soil of fungicide.
Example 5:
the compounding agent comprises the following components in percentage by weight: the mugwort powder is 37.5g/kg soil, the active carbon is 47.0g/kg soil, and the fungicide is 0.15g/kg soil.
Example 6:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of the reed-like powder, 47.0g/kg of soil of the activated carbon and 0.47g/kg of soil of the fungicide.
Example 7:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of tea strip wood leaf powder, 37.5g/kg of soil of active carbon and 0.15g/kg of soil of fungicide.
Example 8:
the compounding agent comprises the following components in percentage by weight: the mugwort powder is 37.5g/kg soil, the active carbon is 37.5g/kg soil, and the fungicide is 0.47g/kg soil.
Example 9:
the compounding agent comprises the following components in percentage by weight: 37.5g/kg of soil of the reed-like powder, 37.5g/kg of soil of the activated carbon and 0.28g/kg of soil of the fungicide.
In examples 1 to 9, the tea strip wood leaf powder, the mugwort powder and the reed-like powder are prepared by respectively taking the tea strip wood leaf, the mugwort and the reed-like overground part, drying and crushing to 200 meshes; the active carbon is: the granular activated carbon AR is analytically pure; the fungicide is: thiophanate-methyl.
Experimental example: planting local leguminous shrub land leech [ Desmodium styracifolium ], [ Desmodium styracifolium ]Desmodium gangeticum (L.) DC.]And the non-leguminous shrub giant knotweed rhizome (Reynoutria japonicahoutt.) competed with the invasive plant Eupatorium adenophorum, respectively. After each treated plant had grown for 3 months, the growth and morphological parameters of Eupatorium adenophorum and the biomass of the native species polygonum cuspidatum were measured.
As a result: compared with the control, the 9 examples all significantly reduce the plant height of the eupatorium adenophorum (27.48-51.35%), the leaf number (12.55-70.56%), the total leaf area (8.29-71.40%), the relative growth rate (13.44-54.48%), and the total biomass (25.73-81.01%); the competitive influence on giant knotweed rhizome and giant mountain leech is respectively reduced by 20.91-32.79% and 20.02-28.03%, and the competitive response of eupatorium adenophorum is increased by 25.73-81.01%, so that the competitive capacity of the eupatorium adenophorum is reduced, and the competitive capacity of local species is improved (table 1). Orthogonal experimental result analysis was performed on 9 examples, and the optimum level of each component was the level at which the growth and competitive power of Eupatorium adenophorum was the lowest, based on the mean comparison at each level of each factor. The best combination pattern of factors and levels was screened: a. the1B1C2Namely 37.5g/kg of soil for tea tree inhibitor, 62.5g/kg of soil for active carbon and 0.28g/kg of soil for fungicide, can reduce the growth and competition of the eupatorium adenophorum to the maximum extent.
TABLE 1 percentage reduction of Eupatorium Adenophorum growth and competition parameters under the examples
Figure 598653DEST_PATH_IMAGE001

Claims (2)

1. A compound agent for inhibiting the growth and competition of Eupatorium adenophorum spreng, which is characterized in that: the compound agent consists of a plant source growth inhibitor, active carbon and a fungicide:
the plant source growth inhibitor is as follows: tea strip leaf powder, folium Artemisiae Argyi powder or similar reed powder; the powder is prepared by respectively taking leaves of tea strip wood, or aerial parts of mugwort and reed rhizome, drying, and pulverizing to 200 mesh;
the active carbon is as follows: the granular activated carbon AR is analytically pure;
the fungicide is: thiophanate-methyl;
the mixture ratio of the plant source growth inhibitor, the active carbon and the fungicide is as follows:
37.5g/kg of soil of tea strip wood leaf powder, 62.5g/kg of soil of active carbon and 0.47g/kg of soil of fungicide; or is
The mugwort powder is 37.5g/kg soil, the active carbon is 62.5g/kg soil, and the fungicide is 0.28g/kg soil; or is
37.5g/kg of soil of the reed-like powder, 62.5g/kg of soil of the active carbon and 0.15g/kg of soil of the fungicide; or is
37.5g/kg of soil of tea strip wood leaf powder, 47.0g/kg of soil of active carbon and 0.28g/kg of soil of fungicide; or is
The mugwort powder is 37.5g/kg of soil, the active carbon is 47.0g/kg of soil, and the fungicide is 0.15g/kg of soil; or is
37.5g/kg of soil of the reed-like powder, 47.0g/kg of soil of the activated carbon and 0.47g/kg of soil of the fungicide; or is
37.5g/kg of soil of tea strip wood leaf powder, 37.5g/kg of soil of active carbon and 0.15g/kg of soil of fungicide; or is
The mugwort powder is 37.5g/kg of soil, the active carbon is 37.5g/kg of soil, and the fungicide is 0.47g/kg of soil; or is
37.5g/kg of soil of the reed-like powder, 37.5g/kg of soil of the activated carbon and 0.28g/kg of soil of the fungicide.
2. The method for preparing the complexing agent for inhibiting the growth and competition of the eupatorium adenophorum as claimed in claim 1, wherein the method comprises the following steps:
the formula of claim 1, wherein the plant-derived growth inhibitor and the activated carbon are added to each kg of soil, the plant-derived growth inhibitor and the activated carbon are uniformly mixed, the fungicide is added to each kg of soil after the mixture is uniformly mixed, and the mixture is uniformly mixed again to prepare the complexing agent; wherein the content of the first and second substances,
the plant source growth inhibitor is as follows: tea strip leaf powder, folium Artemisiae Argyi powder or similar reed powder;
the active carbon is: the granular activated carbon AR is analytically pure;
the fungicide is: thiophanate-methyl.
CN201811261445.XA 2018-10-26 2018-10-26 Complexing agent for inhibiting growth and competition of Eupatorium adenophorum and preparation method thereof Active CN109221301B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101715785A (en) * 2009-11-26 2010-06-02 李翠兰 Special growth inhibitor for Eupatorium adenophorum
CN102090419A (en) * 2010-11-12 2011-06-15 四川鑫穗生物科技有限公司 Growth inhibitor of eupatorium adenophorum

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101715785A (en) * 2009-11-26 2010-06-02 李翠兰 Special growth inhibitor for Eupatorium adenophorum
CN102090419A (en) * 2010-11-12 2011-06-15 四川鑫穗生物科技有限公司 Growth inhibitor of eupatorium adenophorum

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