CN109212199A - A kind of Troponin I detection kit - Google Patents

A kind of Troponin I detection kit Download PDF

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Publication number
CN109212199A
CN109212199A CN201810911373.2A CN201810911373A CN109212199A CN 109212199 A CN109212199 A CN 109212199A CN 201810911373 A CN201810911373 A CN 201810911373A CN 109212199 A CN109212199 A CN 109212199A
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CN
China
Prior art keywords
reagent
troponin
detection kit
concentration
antibody
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Pending
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CN201810911373.2A
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Chinese (zh)
Inventor
黄信用
吕崇翔
曹春梅
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JINHUA KINGS BIOLOGICAL TECHNOLOGY Co Ltd
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JINHUA KINGS BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201810911373.2A priority Critical patent/CN109212199A/en
Publication of CN109212199A publication Critical patent/CN109212199A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/32—Cardiovascular disorders
    • G01N2800/324—Coronary artery diseases, e.g. angina pectoris, myocardial infarction

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

The invention discloses a kind of Troponin I detection kits, including R1 reagent and R2 reagent, the R1 reagent include glycine buffer 80-120mmol/L, polyethylene glycol 50-60mmol/L, poly- carboxyl ethylene glycol amino 1-5g/L, through overheat inactivation after bovine serum albumin(BSA) 0.5-1g/L, donkey serum 30-50mmol/L, 1% concentration NP-40；The R2 reagent includes the Peracetic acid of glycine buffer 100mmol/L, the latex particle 0.1%-0.3% of the anti-human Troponin I antibody of absorption, octyl phenol polyoxyethylene ether 50-70mmol/L, formamide 25-30mmol/L, hydrogen peroxide 10-20mmol/L, 0.5-1.2% that concentration is 5%；A kind of Troponin I detection kit is made, with strong antijamming capability, while having the effect of inhibiting itself nonspecific reaction and high sensitivity.

Description

A kind of Troponin I detection kit
Technical field
The present invention relates to a kind of Troponin I detection kits.
Background technique
Troponin (Tn) is the regulatory protein of contraction of muscle tissue, on the actin filament of contractile protein, in muscle It shrinks and diastole plays important adjustment effect in the process.
Troponin is the distinctive antigen of cardiac muscle cell, is degraded from cardiac muscle fibre in myocardial cell injury.Blood CTn raising reflects myocardial cell damage in clear, and specificity is above previous common myocardial enzymes with sensibility.
Troponin is the marker of myocardial damage necrosis, and the diagnosis and risk stratification to acute myocardial infarction AMI have important Clinical meaning.Troponin value increase prompt myocardial damage, it is seen that in acute myocardial infarction AMI, unstable angina, pulmonary infarction, Heart failure and other lead to disease such as pancreatitis, connective tissue disease of myocardial damage etc., numerical value is higher, and damage range is got over Extensively, in patients of acute myocardial infarction, 3~6 hours start to discharge, and peak within 10~24 hours, and troponin is increased to combine and be lacked Blood trouble evidence facilitates I type myocardial infarction early diagnosis and therapy.
It is mainly on the market troponin detection kit for detecting myoglobins, during the reaction, it may appear that very More nonspecific reactions, and these nonspecific reactions itself are to use mixing examination because of human body itself innate immune reaction Agent reaction is these nonspecific reactions meeting normal reagent reaction process of severe jamming, finally results in detection structure inaccuracy, grinds Study carefully proof, in all reagents, generates nonspecific reaction and be up to 40% or so, nonspecific reaction includes endogenous and external source Property two major classes, therefore, it is necessary to inhibit autoimmune antibody to troponin detection reaction impact.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of strong antijamming capabilities, while having inhibition itself non-specific The Troponin I detection kit of reaction and high sensitivity.
To solve the above problems, the present invention adopts the following technical scheme: a kind of Troponin I detection kit, including R1 Reagent and R2 reagent, the R1 reagent include glycine buffer 80-120mmol/L, polyethylene glycol 50-60mmol/L, poly- carboxylic It is ethyl glycol amino 1-5g/L, the bovine serum albumin(BSA) 0.5-1g/L after overheat inactivation, donkey serum 30-50mmol/L, 1% dense The NP-40 of degree；The R2 reagent includes the latex particle of glycine buffer 100mmol/L, the anti-human Troponin I antibody of absorption 0.1%-0.3%, octyl phenol polyoxyethylene ether 50-70mmol/L, formamide 25-30mmol/L, the hydrogen peroxide that concentration is 5% The Peracetic acid of 10-20mmol/L, 0.5-1.2%；
Further embodiment are as follows: in the R1 reagent and R2 reagent glycine buffer be 25 ± 1 °, PH be 6.2 it is molten Liquid.
Further embodiment are as follows: the R1 reagent and R2 reagent volume ratio are 2-2.3:1.
Further embodiment are as follows: the partial size of the latex particle of the anti-human Troponin I antibody of absorption is 50-60nm.
The beneficial effects of the present invention are:
The present invention uses R1 reagent and R2 reagent, and R1 reagent includes glycine buffer, polyethylene glycol, poly- carboxyl ethylene glycol Amino, the bovine serum albumin(BSA) after overheat inactivation, donkey serum, NP-40；R2 reagent include glycine buffer, absorption it is anti-human The latex particle of Troponin I antibody, octyl phenol polyoxyethylene ether, formamide, hydrogen peroxide, Peracetic acid, by sufficiently mixed After conjunction, with strong antijamming capability, while having the advantages that inhibit itself nonspecific reaction and high sensitivity.
Specific embodiment
The preferred embodiment of the present invention is described in detail below so that advantages and features of the invention can be easier to by It will be appreciated by those skilled in the art that so as to make a clearer definition of the protection scope of the present invention.
Embodiment one
A kind of Troponin I detection kit, including R1 reagent and R2 reagent, the R1 reagent includes glycine buffer 80mmol/L, polyethylene glycol 60mmol/L, poly- carboxyl ethylene glycol amino 3g/L, the bovine serum albumin(BSA) after overheat inactivation 0.5g/L, donkey serum 30mmol/L, 1% concentration NP-40；The R2 reagent includes glycine buffer 100mmol/L, absorption Latex particle 0.3%, octyl phenol polyoxyethylene ether 70mmol/L, the formamide 25mmol/L, concentration of anti-human Troponin I antibody For 5% hydrogen peroxide 15mmol/L, 0.5% Peracetic acid.
Embodiment two
A kind of Troponin I detection kit, including R1 reagent and R2 reagent, the R1 reagent includes glycine buffer 100mmol/L, polyethylene glycol 50mmol/L, poly- carboxyl ethylene glycol amino 3g/L, the bovine serum albumin(BSA) 1g/ after overheat inactivation L, donkey serum 50mmol/L, 1% concentration NP-40；The R2 reagent include glycine buffer 100mmol/L, absorption it is anti-human The latex particle 0.3% of Troponin I antibody, octyl phenol polyoxyethylene ether 50mmol/L, formamide 25mmol/L, concentration are 5% hydrogen peroxide 20mmol/L, 0.5% Peracetic acid.
Embodiment three
A kind of Troponin I detection kit, including R1 reagent and R2 reagent, the R1 reagent includes glycine buffer 110mmol/L, polyethylene glycol 55mmol/L, poly- carboxyl ethylene glycol amino 2g/L, the bovine serum albumin(BSA) 1g/ after overheat inactivation L, donkey serum 50mmol/L, 1% concentration NP-40；The R2 reagent include glycine buffer 100mmol/L, absorption it is anti-human The latex particle 0.3% of Troponin I antibody, octyl phenol polyoxyethylene ether 50mmol/L, formamide 30mmol/L, concentration are 5% hydrogen peroxide 20mmol/L, 1% Peracetic acid.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any The change or replacement expected without creative work should all be covered subject to this practical new protection scope.

Claims (4)

1. a kind of Troponin I detection kit, it is characterised in that: including R1 reagent and R2 reagent, the R1 reagent includes sweet Propylhomoserin buffer 80-120mmol/L, polyethylene glycol 50-60mmol/L, poly- carboxyl ethylene glycol amino 1-5g/L, through overheat inactivate Rear bovine serum albumin(BSA) 0.5-1g/L, donkey serum 30-50mmol/L, 1% concentration NP-40；The R2 reagent includes sweet ammonia Acid buffer 100mmol/L, the latex particle 0.1%-0.3% of the anti-human Troponin I antibody of absorption, octyl phenol polyoxyethylene ether The peroxide second of 50-70mmol/L, formamide 25-30mmol/L, hydrogen peroxide 10-20mmol/L, 0.5-1.2% that concentration is 5% Acid.
2. a kind of Troponin I detection kit according to claim 1, it is characterised in that: the R1 reagent and R2 examination Glycine buffer is 25 ± 1 ° in agent, the solution that PH is 6.2.
3. a kind of Troponin I detection kit according to claim 1, it is characterised in that: the R1 reagent and R2 examination Agent volume ratio is 2-2.3:1.
4. a kind of Troponin I detection kit according to claim 1, it is characterised in that: the anti-human flesh calcium of absorption The partial size of the latex particle of protein I antibody is 50-60nm.
CN201810911373.2A 2018-08-11 2018-08-11 A kind of Troponin I detection kit Pending CN109212199A (en)

Priority Applications (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114295825A (en) * 2021-12-01 2022-04-08 柏荣诊断产品（上海）有限公司 High-sensitivity specific transmission-scattering integrated troponin I latex turbidimetric detection kit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102749460A (en) * 2012-07-27 2012-10-24 北京恩济和生物科技有限公司 Troponin detection kit and preparing method thereof
CN103185798A (en) * 2011-12-27 2013-07-03 苏州德沃生物技术有限公司 Turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement and use method thereof
CN104181308A (en) * 2014-08-22 2014-12-03 山东博科生物产业有限公司 Stable troponin I detection reagent
CN104330575A (en) * 2014-11-17 2015-02-04 中国人民解放军第三0五医院 Troponin I detection reagent and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103185798A (en) * 2011-12-27 2013-07-03 苏州德沃生物技术有限公司 Turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement and use method thereof
CN102749460A (en) * 2012-07-27 2012-10-24 北京恩济和生物科技有限公司 Troponin detection kit and preparing method thereof
CN104181308A (en) * 2014-08-22 2014-12-03 山东博科生物产业有限公司 Stable troponin I detection reagent
CN104330575A (en) * 2014-11-17 2015-02-04 中国人民解放军第三0五医院 Troponin I detection reagent and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114295825A (en) * 2021-12-01 2022-04-08 柏荣诊断产品（上海）有限公司 High-sensitivity specific transmission-scattering integrated troponin I latex turbidimetric detection kit
CN114295825B (en) * 2021-12-01 2024-08-02 柏荣诊断产品（上海）有限公司 High-sensitivity specific transparent scattering integrated troponin I latex turbidimetry detection kit

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