CN109212173A - A kind of grisped grass carp brittleness stage division based on In vivo detection - Google Patents
A kind of grisped grass carp brittleness stage division based on In vivo detection Download PDFInfo
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Abstract
The present invention relates to a kind of grisped grass carp brittleness stage division based on In vivo detection, it include: that (1) acquires different brittle stage grisped grass carp blood samples and with a batch of Blood of Ctenopharyngodon sample and muscle sample, muscle is pre-processed, Taste evaluation brittleness grade；(2) alkaline phosphatase activities, the content of complement C 3, lymphocytes in blood content for detecting blood sample, establish the physiochemical indice database of grisped grass carp；(3) the nonstandardized technique Bayes discriminant function based on physiochemical indice is established；(4) determined using the brittleness grade that Bayes discriminant function carries out grisped grass carp muscle to be determined.The method that the present invention realizes live fish detection grisped grass carp brittleness, being capable of the objective reflection and evaluation grisped grass carp embrittlement effect with high-accuracy of energy when not having to kill fish.
Description
Technical field
The present invention relates to a kind of grisped grass carp brittleness stage division more particularly to a kind of grisped grass carp brittleness based on In vivo detection
Stage division belongs to aquatic product quality detection field.
Background technique
In China, the cultivation of grass carp is that low-income groups relies effective production method of livelihood for a long time, with society
Expanding economy, grass carp cultivation practitioner are not only satisfied with grass carp in the low level price market of bulk product, also attempt to expand
The market share of the grass carp in high-end aquatic products, Chinese Government are also promoting aquaculture upgrading and transformation strategy.
Grisped grass carp is exactly the exploration for most having remarkable result in " upgrading synergy, decrement increase income ".Grisped grass carp (is called brittle grass
Fish) as the Chinese Special Breed object with certain industry size, it is at grass carp (Ctenopharyngodon idellus)
The moment of adult fish culture is that single bait source feeds name spy's water made of cultivation with natural plants semen viciae fabae (Vicia faba)
Product.In production, common grass carp ingest semen viciae fabae become grisped grass carp process be referred to as " embrittlement process ", embrittlement after grass carp
Referred to as grisped grass carp.Grisped grass carp has widened the selling market of grass carp, and the market price of usual grisped grass carp is 2 times of common grass carp, should
Product also obtains national " geography symbol product " protection, and product also gradually extends to all parts of the country, part by Mountain area in Guangdong
It is sold to country in Southeast Asia, or even has and sells to North America on a small quantity.
In the production practices of grisped grass carp, often there is phenomena such as " not crisp ", " half is crisp ", seriously restrict its industry development.Solution
Certainly the premise of this problem is the brittleness grade scale for needing specific, science grisped grass carp.Studies have pointed out that grass carp embrittlement
Journey is the physiology course of progressive tissue lesion, and with apparent feed (semen viciae fabae) intergal dose --- effect reaction
Toxicology rule, the functional lesion including each system organ, especially compromised immune evil are the most obvious.
There is scholar to find hardness, the sense organ brittleness significant positive correlation of chewiness and grisped grass carp, and by grisped grass carp flesh
Meat brittleness is divided into " not crisp enough ", " crisp ", " especially crisp " three grades, and still, this method needs killing grisped grass carp and taking its flesh
On the basis of meat is analyzed, the whole fish for being unfavorable for grisped grass carp is sold and is transported by this.
Summary of the invention
Against the above deficiency, the present invention provides a kind of based on In vivo detection, minimally invasive grisped grass carp brittleness stage division.
Inventor the study found that semen viciae fabae group Serum alkaline phosphatase activity, content of complement C 3, lymphocyte quantity not
It is suppressed with embrittlement period and there is apparent difference.On the basis of finding herein, inventor is from analysis of experimental data table
Bright, in the case where not killing fish, living body extracts grisped grass carp blood, establishes and is based on alkaline phosphatase, content of complement C 3, lymphocyte
The nonstandardized technique Bayes techniques of discriminant analysis of 3 sero-immunity indexs of quantity can reflect the uniqueness of grisped grass carp muscle brittleness indirectly
Quality.
The present invention reaches above-mentioned purpose by following scheme:
The present invention provides a kind of grisped grass carp brittleness stage division based on In vivo detection, comprising:
(1) different brittle stage grisped grass carp blood samples are acquired and with a batch of Blood of Ctenopharyngodon sample, and acquires these
Muscle sample around grisped grass carp and the dorsal fin of grass carp, pre-processes muscle, the brittleness etc. of these muscle of Taste evaluation
Grade；
(2) alkaline phosphatase activities, the content of complement C 3, lymphocytes in blood content for detecting blood sample, are established crisp
The physiochemical indice database of meat grass carp；
(3) the nonstandardized technique Bayes discriminant function based on physiochemical indice is established；
(4) determined using the brittleness grade that Bayes discriminant function carries out grisped grass carp muscle to be determined；
Wherein, the nonstandardized technique Bayes discriminant function is
| Rank | Corresponding classification identification function |
| 1 grade | Y1=10.423*X1+5.835*X2+2734.986*X3-686.913 |
| 2 grades | Y2=8.569*X1+5.702*X2+2752.824*X3-647.723 |
| 3 grades | Y3=7.190*X1+4.047*X2+2328.126*X3-393.298 |
| 4 grades | Y4=5.542*X1+3.184*X2+1384.031*X3-194.902 |
| 5 grades | Y5=5.444*X1+3.462*X2+1837.352*X3-261.325 |
Alkaline phosphatase activities, content of complement C 3, the lymphocytes in blood for detecting grisped grass carp blood sample to be determined contain
Amount, brings the physiochemical indice data of grisped grass carp to be determined into above-mentioned nonstandardized technique Bayes identification function respectively and obtains five grades
Other resulting value, compares five resulting values, and the corresponding rank of maximum resulting value is grisped grass carp muscle brittleness grade to be measured.
Wherein, the grisped grass carp muscle brittleness of Taste evaluation is to establish nonstandardized technique Bayes discriminant function in step (1)
When provide required for hierarchical type.
Wherein, the grisped grass carp in the step (1) is formed completely needs to feed semen viciae fabae about 4 months time, it is preferred that
The brittle stage grisped grass carp blood sample of the difference acquired in step (1) and muscle sample are that sampling in every brittle one month is primary, altogether
Four times, including the muscle sample around acquisition blood sample and dorsal fin.
It is further preferred that the blood sample of the step (1) includes the blood sample and same batch 6 of 6 brittle grass carps
The blood sample of common grass carp, muscle include 6 common grass carps of dorsal muscles muscle samples and same batch of 6 brittle grass carps
Dorsal fin Surrounding muscles sample.
It is further preferred that the blood sample of the step (1) includes the blood sample and same batch 6 of 6 brittle grass carps
The blood sample of common grass carp, muscle include 6 common grass carps of dorsal muscles muscle samples and same batch of 6 brittle grass carps
Dorsal fin Surrounding muscles sample acquires four months, and the acquisition of each moon is primary.
The step (1) carries out pretreated purpose to muscle and is to make the brittleness grade that can carry out muscle to it to reflect
It is fixed, it is preferred that described to carry out pretreatment to muscle include that cooked processing is carried out to muscle, for example including being set muscle with steaming lattice
In on boiling water bath, taken out after steaming 5min with cover, at room temperature natural cooling 20min.
The brittleness grade of these muscle of the Taste evaluation of the step (1) includes that the brittleness grade of muscle is divided into 5 etc.
Grade, is respectively as follows: 1 grade " completely not crisp "；2 grades " not crisp enough "；3 grades " more crisp "；4 grades " crisp ", 5 grades " highly brittle ".
Preferably, the brittleness level identification of the muscle of every tail fish includes at least taking 5 samples crisp for 5 taster's assessments
Degree.
Preferably, the brittleness grade of the Taste evaluation muscle of the step (1) is the brittleness etc. of expert's Taste evaluation muscle
Grade.
Preferably, the acquisition of the blood sample of the step (1) are as follows: fish is anaesthetized, such as after being anaesthetized fish with eugenol,
Behind 75% alcohol disinfecting fish stern position, it is inserted into, such as inclination insertion, is touched at fish body side line lower section 2cm with 10ml syringe
Micro- inclined insertion tail vein after to vertebra extracts 2ml blood in anticoagulant tube, and 10ml blood is respectively prepared in blood collection tube
Whole blood and blood serum sample.
Preferably, the alkaline phosphatase activities in the step (2), content of complement C 3 measuring method surveyed using sero-enzyme
Determine kit to be measured to obtain, such as the Determination of serum enzyme kit of Bioengineering Research Institute is built up in Nanjing.
Preferably, the physiochemical indice database of the grisped grass carp in the step (2) includes alkaline phosphatase activities, Complement C_3
The physiochemical indice database of content, lymphocytes in blood content.
It is further preferred that the physiochemical indice database of the grisped grass carp in the step (2) is different crisp meat of brittle stage
Grass carp blood sample and physiochemical indice data with a batch of Blood of Ctenopharyngodon sample totally 48 samples, data are as shown in table 1.
The physiochemical indice data of the brittle grass carp group of table 1 and common grass carp group
Also, on the basis of above-mentioned table 1, the average physiochemical indice data of grisped grass carp are obtained, the results are shown in Table 2.
The physiochemical indice overall situation of the brittle grass carp group of table 2 and common grass carp group
Note: with a line difference capitalization indicate the different breeding stage physiochemical indice there were significant differences (P < 0.05),
The different lowercase of same row indicates that there were significant differences (P < 0.05) in same culturing time.GC is common grass carp group, CGC
For brittle grass carp group, similarly hereinafter.
As can be drawn from Table 2,3 physiochemical indices of different brittle stage grisped grass carps have apparent difference (P < 0.05),
3 haematogenic immunity index contents of grisped grass carp in same embrittlement stage are substantially less than common grass carp (P < 0.05), nonstandard conducive to establishing
Standardization Bayes discriminant function carries out discriminant analysis.
The database that result described in table 1 is established and verified as nonstandardized technique Bayes discriminant function.
The present invention carries out Accuracy Verification discovery using the Bayes discriminant function established, and discriminant function of the invention carries out
There is at least 93.8% or more accuracy when grisped grass carp brittleness grade assessment, be especially judged to reaching when 2 grades or more grisped grass carps
100% accuracy.
The above results explanation, nonstandardized technique Bayes discriminant function of the invention are differentiating grisped grass carp muscle brittleness time stage
Correctness is excellent.
Compared with the prior art, the beneficial effects of the invention are that:
The method that the present invention realizes live fish detection grisped grass carp brittleness, passes through the physiochemical indice of common grass carp and grisped grass carp
Heterogeneity, and the existing a large amount of experimental data of inventor is combined, select alkaline phosphatase activities, Complement C_3 and blood woods bar
Index of the content of cell as comprehensive identification and reflection grisped grass carp brittleness, so as to the energy visitor when not having to kill fish
See the reflection and evaluation grisped grass carp embrittlement effect with high-accuracy.
The present invention can be vast grisped grass carp raiser and customer service, to produce and select crisp meat best in quality
Grass carp；Meanwhile the production upper brittle time that can help timely, the quick control grisped grass carp of grisped grass carp raiser, brittle effect, how
When out the pool to save production cost, improve its economic benefit it is irregular to can solve grisped grass carp fishery -ies product meat quality in the market
Uneven problem.
Specific embodiment
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1:
A kind of grisped grass carp brittleness stage division based on In vivo detection, steps are as follows:
(1) different brittle stage grisped grass carp blood samples are acquired and with a batch of Blood of Ctenopharyngodon sample, and acquires these
Muscle sample around grisped grass carp and the dorsal fin of grass carp, muscle is placed on boiling water bath, is taken out after steaming 5min with cover, in room temperature
Lower natural cooling 20min, the brittleness grade of these muscle of Taste evaluation at least take 5 samples to assess brittleness for 5 tasters,
The brittleness grade of muscle is divided into 5 grades, is respectively as follows: 1 grade " completely not crisp "；2 grades " not crisp enough "；3 grades " more crisp "；4 grades
" crisp ", 5 grades " highly brittle "；
Wherein, grisped grass carp is formed completely needs to feed semen viciae fabae about 4 months time, and sampling in every brittle one month is primary,
Totally four times, including the muscle sample around acquisition blood sample and dorsal fin, blood sample includes the blood samples of 6 brittle grass carps and same
The blood sample of one 6 common grass carps of batch, the dorsal muscles muscle samples and same batch 6 that muscle includes 6 brittle grass carps are commonly
The dorsal fin Surrounding muscles sample of grass carp；
The acquisition of blood sample specifically: after fish is anaesthetized fish with eugenol, behind 75% alcohol disinfecting fish stern position,
It is inserted at fish body side line lower section 2cm with 10 ml syringes, such as inclination insertion, encounters micro- inclined insertion tail vein after vertebra, take out
Take 2ml blood in anticoagulant tube, whole blood and blood serum sample is respectively prepared in blood collection tube in 10ml blood.
(2) the Determination of serum enzyme kit detection of alkaline phosphatase activity and complement of Bioengineering Research Institute are built up with Nanjing
C3 content establishes grisped grass carp physiochemical indice database with the content of Automaticbloodanalysesmachine detection lymphocytes in blood,
Physiochemical indice database include alkaline phosphatase activities, content of complement C 3, lymphocytes in blood content physiochemical indice data
Library, specially different brittle stage grisped grass carp blood samples and the blood with a batch of Blood of Ctenopharyngodon sample totally 48 samples
Achievement data, data are as shown in Table 1 above.
Also, on the basis of above-mentioned table 1, the average physiochemical indice data of grisped grass carp are obtained, as a result such as above-mentioned 2 institute of table
Show.
As can be drawn from Table 2,3 physiochemical indices of different brittle stage grisped grass carps have apparent difference (P < 0.05),
3 haematogenic immunity index contents of grisped grass carp in same embrittlement stage are substantially less than common grass carp (P < 0.05), nonstandard conducive to establishing
Standardization Bayes discriminant function carries out discriminant analysis.
The database that result described in table 1 is established and verified as nonstandardized technique Bayes discriminant function.
(3) the nonstandardized technique Bayes discriminant function based on physiochemical indice is established, detailed process is as follows: utilizing the number of table 1
According to opening SPSS system successively clicks analysis (Analyze)-classification (Classify)-discriminant analysis (Discriminant
Analysis), after opening discriminant analysis (Discriminant Analysis) dialog box, in grouping variable (Grouping
Variable minimum value (Minimun) filling 1 in), maximum value (Maximum) filling are 5, independent variable Indepengdents choosing
Select X1(lymphocyte content), X2(alkaline phosphatase activities) and X3(content of complement C 3)；Then statistic is clicked again
(Statistics), in the dialog box of pop-up, choose Fisher ' s and do not standardize (Unstandardized), click after
Continuous (Continue) is finally clicked and is determined (OK).Coefficient (the Classification of nonstandardized technique Bayes discriminant function
Function Coefficients) it the results are shown in Table 3, according to table 3 as a result, the nonstandardized technique Bayes discrimination formula after arranging is shown in
Shown in table 4.
The coefficient of 3 nonstandardized technique Bayes identification and classification function of table
4 nonstandardized technique Bayes discriminant function of table
| Rank | Corresponding classification identification function |
| 1 grade | Y1=10.423*X1+5.835*X2+2734.986*X3-686.913 |
| 2 grades | Y2=8.569*X1+5.702*X2+2752.824*X3-647.723 |
| 3 grades | Y3=7.190*X1+4.047*X2+2328.126*X3-393.298 |
| 4 grades | Y4=5.542*X1+3.184*X2+1384.031*X3-194.902 |
| 5 grades | Y5=5.444*X1+3.462*X2+1837.352*X3-261.325 |
Note: X1For lymphocyte content, unit 109.L-1；X2For alkaline phosphatase activities, unit u.mol-1；X3
For content of complement C 3, unit g.l-1, similarly hereinafter.
The nonstandardized technique Bayes techniques of discriminant analysis used in step (3) is with different brittle stage grisped grass carp muscle brittleness
Under the premise of classification has determined (as shown in Table 1, expert sensory's brittleness grade assessment), according to grisped grass carp in embrittlement process
Haematogenic immunity index and the heterogeneity of common grass carp differentiate one kind of grisped grass carp muscle brittleness hierarchical type attaching problem to be measured
Multivariate statistical analysis method.
(4) it goes to differentiate the brittleness grade of grisped grass carp muscle to be determined using nonstandardized technique Bayes discriminant function.
Alkaline phosphatase activities, content of complement C 3, the lymphocytes in blood for detecting grisped grass carp blood sample to be determined contain
Amount, the nonstandardized technique Bayes identification function that 3 physiochemical indice data of grisped grass carp to be determined bring above-mentioned table 3 into respectively is obtained
To the resulting value of five ranks, compare five resulting values, the corresponding rank of maximum resulting value is that grisped grass carp muscle to be measured is crisp
Spend grade.
Test example 1:
It is (i.e. above-mentioned to establish in functional procedure that the nonstandardized technique Bayes discriminant function obtained using table 4 carries out self-authentication
Sample data verifying), decision function determines whether grade is consistent with expert sensory's grade assessment, and the results are shown in Table 5.
The self-authentication result of 5 nonstandardized technique Bayes discriminant function of table
Table 5 the results show that after using nonstandardized technique Bayes discriminant function, 93.8% individual nonstandardized technique Bayes
Discriminant function differentiate after classification situation it is consistent with expert sensory's grade assessment, wherein number be 13,14 and 39 differentiations have it is a little
Error, but error degree is little, in addition, the criteria function effect can reach when grisped grass carp muscle brittleness reaches 2 grades or more
100%, this illustrates that the nonstandardized technique Bayes discriminant function established is differentiating that grisped grass carp muscle brittleness time stage correctness is excellent
It is good, it can be used for the muscle brittleness grade assessment of grisped grass carp.
Test example 2
It is as follows that new sample verifying is carried out to nonstandardized technique Bayes criteria function formula (table 4) of the invention:
Fresh and alive grisped grass carp blood sample to be determined is acquired first, detects the alkaline phosphatase activities in blood, Complement C_3 contains
The content of amount and lymphocyte.Above-mentioned physiochemical indice data are brought into nonstandardized technique Bayes identification function in table 4 to carry out
Resulting value is compared in discriminant analysis, using the maximum grade of resulting value as grisped grass carp muscle brittleness grade to be determined.Meanwhile shortly
Muscle near grisped grass carp dorsal fin invites expert's Taste evaluation grisped grass carp muscle brittleness grade to be measured, to verify the nonstandard of foundation
The accuracy of standardization Bayes discriminant function.The results are shown in Table 6.
Wherein, blood sample acquisition is as follows: after being anaesthetized fish with eugenol, sterilizing fish stern position with 75% cotton ball soaked in alcohol
Afterwards, the 2cm below fish body side line of the syringe with 10ml with syringe needle goes out to tilt insertion, and micro- partially pluggable tail is quiet after encountering vertebra
Arteries and veins extracts 2ml blood in anticoagulant tube, and whole blood and blood serum sample is respectively prepared in blood collection tube in 10ml blood.
Wherein, expert's Taste evaluation grisped grass carp muscle brittleness grade to be measured is as follows: taking the muscle near dorsal fin, is set with steaming lattice
It on boiling water bath, is taken out after steaming 5min with cover, at room temperature natural cooling 20min, invites expert's Taste evaluation grisped grass carp muscle
Brittleness, expert are to invite 5 veteran experts (male 35-50 years old) for being engaged in grisped grass carp industry from Zhongshan City, Guangdong Province,
Award grading evaluation to the brittleness of different muscle: 1 grade is " completely not crisp "；2 grades are " not crisp enough "；3 grades are " more crisp "；4 grades are
" crisp ", 5 grades are " highly brittle ".Every tail fish takes 5 samples to assess brittleness for 5 experts around dorsal fin.
6 grisped grass carp muscle brittleness of table is classified situation
The above results show that the grisped grass carp brittleness stage division of the invention based on In vivo detection can be used for brittleness classification
Detection, accuracy is high, can be used as judgment basis, gives feeding guidance suggestion.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and
Its design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (9)
1. a kind of grisped grass carp brittleness stage division based on In vivo detection characterized by comprising
(1) different brittle stage grisped grass carp blood samples are acquired and with a batch of Blood of Ctenopharyngodon sample, and acquires these crisp meat
Muscle sample around the dorsal fin of grass carp and grass carp, pre-processes muscle, the brittleness grade of these muscle of Taste evaluation；
(2) alkaline phosphatase activities, the content of complement C 3, lymphocytes in blood content for detecting blood sample, establish grisped grass carp
Physiochemical indice database；
(3) the nonstandardized technique Bayes discriminant function based on physiochemical indice is established；
(4) determined using the brittleness grade that Bayes discriminant function carries out grisped grass carp muscle to be determined；
Wherein, the nonstandardized technique Bayes discriminant function is
The alkaline phosphatase activities, content of complement C 3, lymphocytes in blood content of grisped grass carp blood sample to be determined are detected, it will
The physiochemical indice data of grisped grass carp to be determined bring above-mentioned nonstandardized technique Bayes identification function into respectively and obtain the institute of five ranks
It must be worth, compare five resulting values, the corresponding rank of maximum resulting value is grisped grass carp muscle brittleness grade to be measured.
2. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The brittle stage grisped grass carp blood sample of the difference acquired in step (1) and muscle sample are that sampling in every brittle one month is primary, altogether
Four times, including the muscle sample around acquisition blood sample and dorsal fin.
3. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The blood sample of step (1) includes the blood sample of 6 common grass carps of blood sample and same batch of 6 brittle grass carps, muscle
The dorsal fin Surrounding muscles sample of 6 common grass carps of dorsal muscles muscle samples and same batch comprising 6 brittle grass carps.
4. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The brittleness grade of these muscle of the Taste evaluation of step (1) includes that the brittleness grade of muscle is divided into 5 grades, is respectively as follows: 1 grade
" completely not crisp "；2 grades " not crisp enough "；3 grades " more crisp "；4 grades " crisp ", 5 grades " highly brittle ".
5. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that every tail
The brittleness level identification of the muscle of fish includes that 5 samples is at least taken to assess brittleness for 5 tasters.
6. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The acquisition of the blood sample of step (1) are as follows: anaesthetize fish, such as after being anaesthetized fish with eugenol, with 75% alcohol disinfecting fish stern
Behind position, it is inserted at fish body side line lower section 2cm with 10ml syringe, such as inclination insertion, encounters micro- inclined insertion tail after vertebra
Vein extracts 2ml blood in anticoagulant tube, and whole blood and blood serum sample is respectively prepared in blood collection tube in 10ml blood.
7. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The measuring method of alkaline phosphatase activities, content of complement C 3 in step (2) is measured using Determination of serum enzyme kit
It arrives.
8. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The physiochemical indice database of grisped grass carp in step (2) includes alkaline phosphatase activities, content of complement C 3, lymphocytes in blood
The physiochemical indice database of content.
9. a kind of grisped grass carp brittleness stage division based on In vivo detection according to claim 1, which is characterized in that described
The physiochemical indice database of grisped grass carp in step (2) is for different brittle stage grisped grass carp blood samples and with a batch of grass carp
The physiochemical indice data of blood sample totally 48 samples, data are as shown in table 1.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113466232A (en) * | 2021-09-01 | 2021-10-01 | 广东省农业科学院动物科学研究所 | Brittle fish meat rapid detection method and system based on computer image |
| CN114793963A (en) * | 2022-06-29 | 2022-07-29 | 广东省农业科学院动物科学研究所 | Big data controlled fish rapid embrittlement environment control method |
| CN116959036A (en) * | 2023-09-21 | 2023-10-27 | 广东省农业科学院动物科学研究所 | Evaluation method for fish embrittlement degree |
| CN117092041A (en) * | 2023-08-22 | 2023-11-21 | 中国水产科学研究院 | Rapid detection method for muscle quality of living carp based on hyperspectral imaging technology |
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| CN113466232A (en) * | 2021-09-01 | 2021-10-01 | 广东省农业科学院动物科学研究所 | Brittle fish meat rapid detection method and system based on computer image |
| CN113466232B (en) * | 2021-09-01 | 2021-11-09 | 广东省农业科学院动物科学研究所 | Brittle fish meat rapid detection method and system based on computer image |
| CN114793963A (en) * | 2022-06-29 | 2022-07-29 | 广东省农业科学院动物科学研究所 | Big data controlled fish rapid embrittlement environment control method |
| CN114793963B (en) * | 2022-06-29 | 2022-09-02 | 广东省农业科学院动物科学研究所 | Big data controlled fish rapid embrittlement environment control method |
| CN117092041A (en) * | 2023-08-22 | 2023-11-21 | 中国水产科学研究院 | Rapid detection method for muscle quality of living carp based on hyperspectral imaging technology |
| CN116959036A (en) * | 2023-09-21 | 2023-10-27 | 广东省农业科学院动物科学研究所 | Evaluation method for fish embrittlement degree |
| CN116959036B (en) * | 2023-09-21 | 2023-11-28 | 广东省农业科学院动物科学研究所 | Evaluation method for fish embrittlement degree |
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