CN109207539A - A kind of flaxseed meal protein extraction technique - Google Patents
A kind of flaxseed meal protein extraction technique Download PDFInfo
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- CN109207539A CN109207539A CN201810955793.0A CN201810955793A CN109207539A CN 109207539 A CN109207539 A CN 109207539A CN 201810955793 A CN201810955793 A CN 201810955793A CN 109207539 A CN109207539 A CN 109207539A
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- flaxseed meal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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Abstract
The invention belongs to food processing technology fields, and in particular to a kind of flaxseed meal protein extraction technique.The flaxseed meal that enzyme inhibitor is added is put into the water-bath kept at a certain temperature and is heated, is stirred, filtering；A certain proportion of distilled water is added into filtered material, stirs；It is stirring evenly and then adding into sodium hydroxide solution, pH is adjusted and reaches certain value, carry out ultrasonication；Crosslinked polyvinylpyrrolidone is added into the supernatant after ultrasonication, then adjusts extracting solution to albumen isoelectric point with hydrochloric acid, carries out ultracentrifugation processing again, collect sediment.The present invention can be improved the content of the crude protein in flaxseed meal by ethyl alcohol-ammonia-n-hexane mixed liquor, material and distilled water ratio, further increases the content of albumen in flaxseed meal, reaches 92% or more.
Description
Technical field
The invention belongs to food processing technology fields, and in particular to a kind of flaxseed meal protein extraction technique.
Background technique
Linseed is the dry mature seed of flax family (Linaceae) linum flax (Linum usitatissimum L),
Alias flaxseed, flax benevolence originate in Mediterranean Region, now have cultivation all over China, but more general with the north and southwest
Time, ease is wild sometimes.Linseed is a kind of plant resources of important dietotherapeutic, is recorded in " Chinese Pharmacopoeia ", sweet in flavor,
It is mild-natured, have moisturize, wind-dispelling and other effects.Modern pharmacological studies have shown that linseed has multiple biological activities, including antitumor work
With, antioxidation, immunosuppressive action and the effects of reducing blood sugar and blood lipid.Linseed is flat oval shape, and long 4-7mm is wide by 2
.5mm left and right, 1 .5mm of thickness, mass of 1000 kernel are 3 .5-11g.Linseed is made of epidermis, shell and cotyledon.0 .1-0 of epidermis thickness
.2mm, the mainly carbohydrates complex compound such as starch.The half that cotyledon accounts for seed weight is slightly more, containing most of oil and albumen
Matter.Oil exists into the cell, and albumen is primarily present in aleurone grain, remaining exists as cell itself structure, point of shell and meat
It is difficult from more.
Currently, China's linseed processing industry is still in the primary stage, product is only limitted to linseed oil, high in traditional handicraft
Temperature, which steams to fry, makes the unsaturated fatty acid in protein denaturation and linseed in linseed go bad and generate undesirable flavor, cake
Dregs of rice part, which is limited the quantity, is added to feed or farmland fertilizer, utilizes valuable plant protein resource well, affects
The exploitation of flax protein resource and application in the food industry.
Summary of the invention
To overcome the defects of present in the prior art, the present invention provides a kind of flaxseed meal protein extraction technique, can solve
Certainly above-mentioned technical problem.
The invention solves technical solutions used by its technical problem to be: designing a kind of flaxseed meal protein extraction work
Skill successively carries out as steps described below:
Step 1: linseed being put into pulverizer and is crushed, and sieving removal part flax seed husk obtains linseed meal, will
Linseed meal is put into the mixed solvent and carries out mentioning oil, filters, can be obtained flaxseed meal；
Step 2: enzyme inhibitor is added into the flaxseed meal of acquisition；
Step 3: the flaxseed meal that enzyme inhibitor is added is put into the water-bath kept at a certain temperature and is heated, is stirred
It mixes, filters；
Step 4: a certain proportion of distilled water being added into filtered material, stirring；It is molten to be stirring evenly and then adding into sodium hydroxide
Liquid adjusts pH and reaches certain value, carries out ultrasonication；
Step 5: crosslinked polyvinylpyrrolidone being added into the supernatant after ultrasonication, is then adjusted and is extracted with hydrochloric acid
Liquid carries out ultracentrifugation processing to albumen isoelectric point again, collects sediment.
As a further optimization solution of the present invention, mixed solvent described in step 1 is mixed for ethyl alcohol-ammonia-n-hexane
Close liquid.
As a further optimization solution of the present invention, enzyme inhibitor described in step 2 is composite plant hydrolase.
As a further optimization solution of the present invention, certain temperature described in step 3 is 50 DEG C.
As a further optimization solution of the present invention, the ratio of material described in step 4 and distilled water is 1:14-1:
35, the pH value is 8-10, and the temperature of ultrasonication is 53 DEG C.
The present invention has the beneficial effects that the present invention passes through ethyl alcohol-ammonia-n-hexane mixed liquor, material and distillation
The ratio of water can be improved the content of the crude protein in flaxseed meal, further increases the content of albumen in flaxseed meal, makes it
Reach 92% or more.The process step of the invention is simple, and in obtained flaxseed meal the purity of albumen can reach 93% with
On.
Specific embodiment
Embodiment one
A kind of flaxseed meal protein extraction technique successively carries out as steps described below:
Step 1: linseed being put into pulverizer and is crushed, and sieving removal part flax seed husk obtains linseed meal, will
Linseed meal is put into the mixed solvent and carries out mentioning oil, filters, can be obtained flaxseed meal；The mixed solvent is ethyl alcohol-ammonia-
The mixed liquor of n-hexane.
Step 2: enzyme inhibitor is added into the flaxseed meal of acquisition；The enzyme inhibitor is composite plant hydrolase.
Step 3: the flaxseed meal that enzyme inhibitor is added is put into the water-bath being maintained at 50 DEG C and is heated, is stirred
It mixes, filters；
Step 4: being added distilled water into filtered material, and the ratio of the material and distilled water is 1:14, stirring；It stirs
Sodium hydroxide solution is added after mixing uniformly, adjusting pH is 8, carries out ultrasonication；The temperature of ultrasonication is 53 DEG C
Step 5: crosslinked polyvinylpyrrolidone being added into the supernatant after ultrasonication, is then adjusted and is extracted with hydrochloric acid
Liquid carries out ultracentrifugation processing to albumen isoelectric point again, collects sediment.
Embodiment two
A kind of flaxseed meal protein extraction technique successively carries out as steps described below:
Step 1: linseed being put into pulverizer and is crushed, and sieving removal part flax seed husk obtains linseed meal, will
Linseed meal is put into the mixed solvent and carries out mentioning oil, filters, can be obtained flaxseed meal；The mixed solvent is ethyl alcohol-ammonia-
The mixed liquor of n-hexane.
Step 2: enzyme inhibitor is added into the flaxseed meal of acquisition；The enzyme inhibitor is composite plant hydrolase.
Step 3: the flaxseed meal that enzyme inhibitor is added is put into the water-bath being maintained at 50 DEG C and is heated, is stirred
It mixes, filters；
Step 4: being added distilled water into filtered material, and the ratio of the material and distilled water is 1:25, stirring；It stirs
Sodium hydroxide solution is added after mixing uniformly, adjusting pH is 9, carries out ultrasonication；The temperature of ultrasonication is 53 DEG C
Step 5: crosslinked polyvinylpyrrolidone being added into the supernatant after ultrasonication, is then adjusted and is extracted with hydrochloric acid
Liquid carries out ultracentrifugation processing to albumen isoelectric point again, collects sediment.
Embodiment three
A kind of flaxseed meal protein extraction technique successively carries out as steps described below:
Step 1: linseed being put into pulverizer and is crushed, and sieving removal part flax seed husk obtains linseed meal, will
Linseed meal is put into the mixed solvent and carries out mentioning oil, filters, can be obtained flaxseed meal；The mixed solvent is ethyl alcohol-ammonia-
The mixed liquor of n-hexane.
Step 2: enzyme inhibitor is added into the flaxseed meal of acquisition；The enzyme inhibitor is composite plant hydrolase.
Step 3: the flaxseed meal that enzyme inhibitor is added is put into the water-bath being maintained at 50 DEG C and is heated, is stirred
It mixes, filters；
Step 4: being added distilled water into filtered material, and the ratio of the material and distilled water is 1:35, stirring；It stirs
Sodium hydroxide solution is added after mixing uniformly, adjusting pH is 10, carries out ultrasonication；The temperature of ultrasonication is 53 DEG C
Step 5: crosslinked polyvinylpyrrolidone being added into the supernatant after ultrasonication, is then adjusted and is extracted with hydrochloric acid
Liquid carries out ultracentrifugation processing to albumen isoelectric point again, collects sediment.
The present invention can be improved in flaxseed meal by ethyl alcohol-ammonia-n-hexane mixed liquor, material and distilled water ratio
Crude protein content, further increase the content of albumen in flaxseed meal, reach 92% or more.Technique step of the invention
It is rapid simple, and the purity of albumen can reach 93% or more in obtained flaxseed meal.
It should be noted last that: the above embodiments are only used to illustrate and not limit the technical solutions of the present invention, although ginseng
It is described the invention in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that: it still can be to this
Invention is modified or replaced equivalently, without departing from the spirit or scope of the invention, or any substitutions,
It is intended to be within the scope of the claims of the invention.
Claims (5)
1. a kind of flaxseed meal protein extraction technique, it is characterised in that: successively carry out as steps described below:
Step 1: linseed being put into pulverizer and is crushed, and sieving removal part flax seed husk obtains linseed meal, will
Linseed meal is put into the mixed solvent and carries out mentioning oil, filters, can be obtained flaxseed meal；
Step 2: enzyme inhibitor is added into the flaxseed meal of acquisition；
Step 3: the flaxseed meal that enzyme inhibitor is added is put into the water-bath kept at a certain temperature and is heated, is stirred
It mixes, filters；
Step 4: a certain proportion of distilled water being added into filtered material, stirring；It is molten to be stirring evenly and then adding into sodium hydroxide
Liquid adjusts pH and reaches certain value, carries out ultrasonication；
Step 5: crosslinked polyvinylpyrrolidone being added into the supernatant after ultrasonication, is then adjusted and is extracted with hydrochloric acid
Liquid carries out ultracentrifugation processing to albumen isoelectric point again, collects sediment.
2. a kind of flaxseed meal protein extraction technique according to claim 1, it is characterised in that: described in step 1 mixed
Bonding solvent is ethyl alcohol-ammonia-n-hexane mixed liquor.
3. a kind of flaxseed meal protein extraction technique according to claim 1, it is characterised in that: enzyme described in step 2
Inhibitor is composite plant hydrolase.
4. a kind of flaxseed meal protein extraction technique according to claim 1, it is characterised in that: one described in step 3
Determining temperature is 50 DEG C.
5. a kind of flaxseed meal protein extraction technique according to claim 1, it is characterised in that: object described in step 4
The ratio of material and distilled water is 1:14-1:35, and the pH value is 8-10, and the temperature of ultrasonication is 53 DEG C.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810955793.0A CN109207539A (en) | 2018-08-21 | 2018-08-21 | A kind of flaxseed meal protein extraction technique |
| AU2018101568A AU2018101568A4 (en) | 2018-08-21 | 2018-10-19 | A kind of flaxseed flake protein extraction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810955793.0A CN109207539A (en) | 2018-08-21 | 2018-08-21 | A kind of flaxseed meal protein extraction technique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109207539A true CN109207539A (en) | 2019-01-15 |
Family
ID=64350541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810955793.0A Pending CN109207539A (en) | 2018-08-21 | 2018-08-21 | A kind of flaxseed meal protein extraction technique |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109207539A (en) |
| AU (1) | AU2018101568A4 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1863816A (en) * | 2003-08-01 | 2006-11-15 | 伯康营养科学(Mb)公司 | Process for preparation of flax protein isolate |
| CN105602701A (en) * | 2016-01-05 | 2016-05-25 | 北京化工大学 | New method for deeply processing flaxseeds |
| CN105918610A (en) * | 2016-04-20 | 2016-09-07 | 武汉天天好生物制品有限公司 | Flaxseed peptides having effects of lowering cholesterol and preparation method thereof |
| CN107557420A (en) * | 2017-08-22 | 2018-01-09 | 无锡初晨生物科技有限公司 | A kind of preparation method of flaxseed meal polypeptide |
| CN108070630A (en) * | 2018-02-13 | 2018-05-25 | 广州利众生物科技有限公司 | A kind of preparation method of high-purity Lins eed protein |
-
2018
- 2018-08-21 CN CN201810955793.0A patent/CN109207539A/en active Pending
- 2018-10-19 AU AU2018101568A patent/AU2018101568A4/en not_active Ceased
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1863816A (en) * | 2003-08-01 | 2006-11-15 | 伯康营养科学(Mb)公司 | Process for preparation of flax protein isolate |
| CN105602701A (en) * | 2016-01-05 | 2016-05-25 | 北京化工大学 | New method for deeply processing flaxseeds |
| CN105918610A (en) * | 2016-04-20 | 2016-09-07 | 武汉天天好生物制品有限公司 | Flaxseed peptides having effects of lowering cholesterol and preparation method thereof |
| CN107557420A (en) * | 2017-08-22 | 2018-01-09 | 无锡初晨生物科技有限公司 | A kind of preparation method of flaxseed meal polypeptide |
| CN108070630A (en) * | 2018-02-13 | 2018-05-25 | 广州利众生物科技有限公司 | A kind of preparation method of high-purity Lins eed protein |
Non-Patent Citations (2)
| Title |
|---|
| 张慧君等: ""响应面优化酶解法提取亚麻粕蛋白的工艺研究"", 《食品工业》 * |
| 胡爱军等: ""超声辅助提取亚麻籽粕分离蛋白工艺研究"", 《粮食与油脂》 * |
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| Publication number | Publication date |
|---|---|
| AU2018101568A4 (en) | 2018-11-29 |
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