CN109207405B - Cadmium-resistant lactic acid bacteria and application thereof - Google Patents
Cadmium-resistant lactic acid bacteria and application thereof Download PDFInfo
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- CN109207405B CN109207405B CN201811235496.5A CN201811235496A CN109207405B CN 109207405 B CN109207405 B CN 109207405B CN 201811235496 A CN201811235496 A CN 201811235496A CN 109207405 B CN109207405 B CN 109207405B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
Abstract
The invention discloses cadmium-resistant lactic acid bacteria and application thereof, and relates to the technical field of cadmium adsorption, wherein the preservation number of the cadmium-resistant lactic acid bacteria is CGMCC NO. 16301. The cadmium-resistant lactic acid bacteria can resist the cadmium concentration of 1000mg/L at most, has the cadmium adsorption rate of over 30 percent on a cadmium solution of 50mg/L under the condition of pH6, and has an inhibiting effect on staphylococcus aureus.
Description
Technical Field
The invention relates to the technical field of cadmium adsorption, and particularly relates to cadmium-resistant lactic acid bacteria and application thereof.
Background
Lactic acid bacteria (lactobacillus) is a probiotic widely used in animal feed and has the functions of promoting animal growth and improving animal intestinal health. Researches in recent years show that the lactobacillus has the function of adsorbing heavy metals and is applied to the treatment of heavy metal pollution of water and soil. Cadmium is one of the heavy metals defined in the current feed hygiene standards. Some domestic and foreign investigations show that cadmium in energy protein feed such as raw material wheat bran, rapeseed meal and the like, mineral raw material stone powder, calcium hydrophosphate and the like exceeds the limit range of the feed sanitary standard, the risk of exceeding the standard of the cadmium in the feed is brought to the animal breeding industry, and the risk of exceeding the standard of the cadmium in animal food is brought at the same time. Cadmium has serious harm to animals and humans and is classified as a grade I carcinogen by the international institute for cancer (IARC). In order to prevent and reduce the harm and risk of cadmium to animals and human beings, a method for reducing the harm of cadmium in feed to animals needs to be found. The currently adopted method comprises the steps of utilizing a plurality of chelating agents such as dimercaptosuccinic acid to chelate cadmium and then discharging cadmium; increasing the supply of necessary trace elements such as iron and the like in the feed, and reducing the deposition of cadmium in animal bodies by competing iron and the like with cadmium for metallothionein; supplementing vitamins such as vC and vE, and reducing oxidative damage of cadmium to body cells. The method has certain disadvantages, the use of a large amount of chelating agent has side effects on organisms, the supplement of trace elements and vitamins has a relieving effect on high-dose cadmium, and the high toxic amount of cadmium in the feed is difficult to achieve under general conditions, so the economical efficiency and the applicability of the method are questionable.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a cadmium-resistant lactic acid bacterium which can live in a high-concentration cadmium environment, has a strong adsorption effect on cadmium and can be used for removing cadmium elements in substrates such as feed, soil, culture medium and the like.
The invention also aims to provide application of the cadmium-resistant lactic acid bacteria.
Another object of the present invention is to provide a cadmium adsorbent.
Another object of the present invention is to provide a method for adsorbing cadmium.
The invention is realized by the following steps:
on one hand, the invention provides cadmium-resistant lactobacillus, the preservation number of which is CGMCC NO. 16301.
The strain is preserved in China general microbiological culture Collection center (CGMCC) in 2018, 8, 15 and addresses: xilu No.1 Hospital No. 3, Beijing, Chaoyang, Beijing, on the North of the Chaoyang district, was taxonomically named: lactobacillus johnsonii (Lactobacillus johnsonii) with the preservation number of CGMCC NO. 16301.
Further, in some embodiments of the present invention, the 16S rRNA gene has a base sequence shown in SEQ ID NO. 1.
Further, in some embodiments of the invention, it has the following bacteriostatic properties: has the property of inhibiting staphylococcus aureus and campylobacter jejuni, and has no property of inhibiting escherichia coli and salmonella.
Further, in some embodiments of the invention, the cadmium-tolerant lactic acid bacteria have a bile salt tolerance concentration of 0.5%.
Further, in some embodiments of the invention, the cadmium-tolerant lactic acid bacteria have a cadmium adsorption rate of more than 30% at a pH of 6.
The inventor of the invention separates a new cadmium-tolerant lactic acid bacterium from intestinal contents of chickens for the first time, the cadmium-tolerant lactic acid bacterium can tolerate the cadmium concentration of up to 1000mg/L, the cadmium adsorption rate of a 50mg/L cadmium solution is more than 30% under the condition that the pH value is 6, and the cadmium-tolerant lactic acid bacterium has an inhibiting effect on staphylococcus aureus. The cadmium-resistant lactic acid bacteria can be used for removing cadmium metal elements in a substrate, and the substrate can be soil, feed, culture medium and other substances needing cadmium removal.
On the other hand, the invention provides the application of the cadmium-resistant lactic acid bacteria in the preparation of a cadmium adsorbent.
In another aspect, the invention provides a cadmium adsorbent comprising the cadmium-resistant lactic acid bacteria.
In another aspect, the present invention provides a method for adsorbing cadmium, which includes: adding the cadmium-resistant lactic acid bacteria into the substrate needing cadmium removal.
Further, in some embodiments of the invention, the pH of the matrix is controlled to be 2.6 to 7.
Further, in some embodiments of the invention, the pH of the matrix is controlled to be 5.8 to 6.2.
The research of the invention finds that the cadmium-resistant lactic acid bacteria have better cadmium adsorption rate under the condition of pH 2.6-7, for example, the cadmium adsorption rate exceeds 30% under the condition of pH6, and the effect is unexpected.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 shows the gram staining results of lactic acid bacteria.
Fig. 2 shows the growth results of lactic acid bacteria that tolerated the maximum cadmium concentration.
Fig. 3 shows lactic acid bacteria: the growth of staphylococcus aureus is inhibited by Cd1-1, Cd3-1, Cd5-2, Cd4-5 and Cd 8-2.
Fig. 4 shows lactic acid bacteria: cd1-1, Cd3-1, Cd5-2, Cd4-5 and Cd8-2 inhibit the growth of Campylobacter jejuni.
FIG. 5 shows the result of an evolutionary tree constructed from the 16S rRNA sequence of Cd3-1 and other strains of the same species.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
Separation and identification of cadmium-tolerant lactic acid bacteria
1 sample was derived from chicken intestinal contents. Taking 0.1g of intestinal contents, and gradually diluting the intestinal contents to 10 degrees by using sterile PBS solution-5Respectively dropping 10 mu L of each-level dilution liquid on an MRS solid culture medium containing 50mg/L of Cd, carrying out anaerobic culture at 37 ℃ for 48h, then picking out a single colony, continuously inoculating the single colony on the MRS solid culture medium containing 100mg/L of Cd, continuously culturing for 48h, then picking out the single colony, repeatedly inoculating the single colony on the MRS solid culture medium containing 100mg/L of Cd, and culturing for 48h to obtain the single colony which is a strain resistant to 100mg/L of cadmium. Named Cd 3-1.
2 identification of lactic acid bacteria
2.1 gram staining
Selecting a single lactic acid bacteria colony, uniformly mixing in 10 μ L sterile PBS solution, coating on a sterile glass slide, rapidly fixing on flame, cooling, performing gram staining procedure, and observing under an inverted microscope with an oil lens to determine gram-positive bacteria (figure 1).
2.216S rRNA biological identification
After the strain obtained in the step 1 is statically cultured for 18h at 37 ℃ in an MRS liquid culture medium, 1mL of culture solution is taken, and the DNA of the strain is extracted according to the requirements of a TaKaRa bacterial DNA extraction kit (No: 9763). The strain 16S rRNA gene was PCR amplified using bacterial 16S rRNA gene universal primers (Table 1). The PCR amplification procedure was: 1min at 95 ℃, 30s at 52 ℃, 2min at 72 ℃, and 35 circulation at 16 ℃. Taking 4 microliter of PCR amplification product, carrying out electrophoresis by using 1% agarose gel, sending the rest PCR product to a biotechnology company for sequencing, and comparing the sequencing result with an NCBI database to determine the lactobacillus species.
TABLE 116S rRNA Gene amplification primers
The 16S rRNA gene sequencing results are as follows (SEQ ID NO. 1):
CATGCAAGTCGAGCGAGCTTGCCTAGATGATTTTAGTGCTTGCACTAAATGAAAC TAGATACAAGCGAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCAAGAGAC TGGGATAACACCTGGAAACAGATGCTAATACCGGATAACAACACTAGACGCATGTCTA GAGTTTGAAAGATGGTTCTGCTATCACTCTTGGATGGACCTGCGGTGCATTAGCTAGTT GGTAAGGTAACGGCTTACCAAGGCAATGATGCATAGCCGAGTTGAGAGACTGATCGG CCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCT TCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGG CTCGTAAAGCTCTGTTGGTAGTGAAGAAAGATAGAGGTAGTAACTGGCCTTTATTTGA CGGTAATTACTTAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG GTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCAATAAGT CTGATGTGAAAGCCTTCGGCTCAACCGGAGAATTGCATCAGAAACTGTTGAACTTGA GTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGA AGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAA GCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGC TAAGTGTTGGGAGGTTTCCGCCTCTCAGTGCTGCAGCTAACGCATTAAGCACTCCGCC TGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAG CGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACAT CCAGTGCAAACCTAAGAGATTAGGTGTTCCCTTCGGGGACGCTGAGACAGGTGGTGC ATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC CCTTGTCATTAGTTGCCATCATTAAGTTGGGCACTCTAATGAGACTGCCGGTGACAAAC CGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGACCTGGGCTACACAC GTGCTACAATGGACGGTACAACGAGAAGCGAACCTGCGAAGGCAAGCGGATCTCTTA AAGCCGTTCTCAGTTCGGACTGTAGGCTGCAACTCGCCTACACGAAGCTGGAATCGCT AGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC CGTCACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGGAGTC AGCCGTC。
the evolutionary tree constructed from the 16s rRNA gene sequence is shown in FIG. 5, and from the results in FIG. 5, it can be seen that Cd3-1 is a novel lactic acid bacterial strain different from the existing one.
2.3 maximum cadmium tolerance of lactic acid bacteria
Inoculating lactobacillus in the above 1 into 5mL MRS liquid culture medium according to 1% of inoculation amount, culturing for 18h, adjusting absorbance to 1(OD600), and gradually diluting to 10-1、10-2、10-3、10-4、10-5Respectively dripping 10 μ L of the mixture into a solution containing 100mg/L, 200mg/L, 400mg/L, 800 mg/L of Cd,Performing anaerobic culture on 1000mg/L MRS solid culture medium at 37 ℃ for 72h to observe results, determining the maximum cadmium tolerance concentration of the lactic acid bacteria, inoculating the lactic acid bacteria strain with the maximum cadmium tolerance concentration on the cadmium-free MRS solid culture medium, and performing anaerobic culture at 37 ℃ for 48 h. The results show that it can tolerate growth with a maximum cadmium concentration of 1000mg/L (FIG. 2).
2.4 adsorption of lactic acid bacteria to cadmium ions in solution
Inoculating lactobacillus in 1% to 5mL MRS liquid culture medium, anaerobic culturing for 18h, centrifuging at 10000r/min for 10 min, removing supernatant, washing with ultrapure water, precipitating, and adjusting absorbance to 1 (OD)600)Taking 0.5mL of solution, centrifuging for 10 minutes at 10000r/min, removing supernatant, adding 0.5mL of 50mg/L cadmium solution with pH of 6.0 for suspension precipitation, oscillating at 37 ℃ at medium speed for 2 hours, centrifuging for 10 minutes at 10000r/min, taking supernatant, diluting by proper times, and measuring the cadmium content in the solution by a graphite furnace method. And (3) calculating the cadmium adsorption rate of the lactic acid bacteria by using the formula (1).
Adsorption rate (%) - (C) of 100%1/C0)×100％ (1)
Wherein C is0Is the initial concentration of cadmium solution, C1The concentration of the cadmium solution in the final supernatant is shown.
The result shows that the absorption rate of the lactobacillus to cadmium of 50mg/L cadmium solution (pH6) is more than 30%.
2.5 bacteriostatic Properties of lactic acid bacteria
Inoculating the lactobacillus in the step 1 into 5mL of MRS liquid culture medium according to the inoculation amount of 1 percent, culturing for 18h, adjusting the absorbance to 1(OD600), putting 1mL of MRS culture medium into a sterile tube, and respectively soaking 6mm sterile filter paper sheets into the MRS culture medium. Adjusting the absorbance of staphylococcus aureus, campylobacter jejuni, escherichia coli and salmonella to 0.08-0.1(OD600), respectively taking 50 mu L of staphylococcus aureus, escherichia coli and salmonella, uniformly coating LB culture medium, uniformly coating MH blood culture medium, respectively paving filter paper sheets on a bacterial culture plate, respectively culturing staphylococcus aureus, escherichia coli and salmonella at 37 ℃ for 24h, and observing the result after the campylobacter jejuni is anaerobically cultured at 42 ℃ for 48h, thereby determining the bacteriostatic property of the lactobacillus. The results show that the lactic acid bacteria can inhibit the growth of staphylococcus aureus and campylobacter jejuni (fig. 3, fig. 4).
2.6 lactic acid bacteria tolerant bile salts
Inoculating lactobacillus in the above 1 into 5mL MRS liquid culture medium according to 1% of inoculation amount, culturing for 18h, adjusting absorbance to 1(OD600), and gradually diluting to 10-1、10-2、10-3、10-4、 10-5And respectively dripping 10 mu L of the culture solution onto MRS solid culture plates containing 0.1%, 0.2%, 0.3%, 0.4% and 0.5% of bovine bile salt, and determining the concentration of bile salt tolerance of the lactic acid bacteria by using the MRS culture plates without bile salt as a control and carrying out anaerobic culture at 37 ℃ for 48h for observation.
The results show that the lactic acid bacteria are resistant to 0.5% bile salts.
In conclusion, the lactobacillus can tolerate the maximum cadmium concentration of 1000mg/L, has the cadmium adsorption rate of more than 30% on a 50mg/L cadmium solution (pH6), tolerates 0.5% of bile salt, and can inhibit the growth of staphylococcus aureus and campylobacter jejuni.
The Cd3-1 strain is preserved in the China general microbiological culture Collection center in 2018, 8 and 15, and the addresses are as follows: xilu No.1 Hospital No. 3, Beijing, Chaoyang, Beijing, on the North of the Chaoyang district, was taxonomically named: the collection number of Lactobacillus johnsonii (Lactobacillus johnsonii) is CGMCC NO. 16301.
Example 2
Adsorption of lactic acid bacteria Cd3-1 on cadmium ions in solution
Inoculating lactic acid bacteria Cd3-1 into 5mL of MRS liquid culture medium according to the inoculation amount of 1 percent, performing anaerobic culture for 18h, centrifuging at 10000r/min for 10 min, removing supernatant, washing with ultrapure water, precipitating, adjusting the absorbance to 1(OD600), taking 0.5mL of solution, centrifuging at 10000r/min for 10 min, removing supernatant, adding 0.5mL of 50mg/L cadmium solution with pH of 2.6, 4.0, 6.0 and 7.0 respectively, suspending and precipitating, oscillating at 37 ℃, shaking at medium speed for 2h, centrifuging at 10000r/min for 10 min, taking supernatant, diluting by proper times, and measuring the cadmium content in the solution by a graphite furnace method. And (3) calculating the cadmium adsorption rate of the lactic acid bacteria by using the formula (1).
Adsorption rate (%) - (C) of 100%1/C0)×100％
Wherein C is0Is the initial concentration of cadmium solution, C1The concentration of the cadmium solution in the final supernatant is shown.
The results (table 2) show that pH has a greater effect on the adsorption of cadmium by the strain. At pH values of 2.6 and 4.0, the adsorption rate of the lactic acid bacteria strain to cadmium in a 50mg/L cadmium solution is lower than 20%, the adsorption rate of cadmium increases along with the increase of the pH value, the adsorption rate of cadmium is the highest at pH value of 6.0 and exceeds 30%, and the adsorption rate of cadmium approaches 30% at pH value of 7.0.
TABLE 2 adsorption rate of Cd3-1 on cadmium solution
| pH | Adsorption rate |
| 2.6 | 17.6±2.1 |
| 4.0 | 18.5±0.2 |
| 6.0 | 31.1±1.6 |
| 7.0 | 28.9±0.3 |
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
SEQUENCE LISTING
<110> scientific research institute for poultry in Jiangsu province
<120> cadmium-resistant lactic acid bacteria and application thereof
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1444
<212> DNA
<213> Artificial sequence
<400> 1
catgcaagtc gagcgagctt gcctagatga ttttagtgct tgcactaaat gaaactagat 60
acaagcgagc ggcggacggg tgagtaacac gtgggtaacc tgcccaagag actgggataa 120
cacctggaaa cagatgctaa taccggataa caacactaga cgcatgtcta gagtttgaaa 180
gatggttctg ctatcactct tggatggacc tgcggtgcat tagctagttg gtaaggtaac 240
ggcttaccaa ggcaatgatg catagccgag ttgagagact gatcggccac attgggactg 300
agacacggcc caaactccta cgggaggcag cagtagggaa tcttccacaa tggacgaaag 360
tctgatggag caacgccgcg tgagtgaaga agggtttcgg ctcgtaaagc tctgttggta 420
gtgaagaaag atagaggtag taactggcct ttatttgacg gtaattactt agaaagtcac 480
ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat 540
tgggcgtaaa gcgagtgcag gcggttcaat aagtctgatg tgaaagcctt cggctcaacc 600
ggagaattgc atcagaaact gttgaacttg agtgcagaag aggagagtgg aactccatgt 660
gtagcggtgg aatgcgtaga tatatggaag aacaccagtg gcgaaggcgg ctctctggtc 720
tgcaactgac gctgaggctc gaaagcatgg gtagcgaaca ggattagata ccctggtagt 780
ccatgccgta aacgatgagt gctaagtgtt gggaggtttc cgcctctcag tgctgcagct 840
aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga 900
cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta 960
ccaggtcttg acatccagtg caaacctaag agattaggtg ttcccttcgg ggacgctgag 1020
acaggtggtg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac 1080
gagcgcaacc cttgtcatta gttgccatca ttaagttggg cactctaatg agactgccgg 1140
tgacaaaccg gaggaaggtg gggatgacgt caagtcatca tgccccttat gacctgggct 1200
acacacgtgc tacaatggac ggtacaacga gaagcgaacc tgcgaaggca agcggatctc 1260
ttaaagccgt tctcagttcg gactgtaggc tgcaactcgc ctacacgaag ctggaatcgc 1320
tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta cacaccgccc 1380
gtcacaccat gagagtctgt aacacccaaa gccggtggga taacctttat aggagtcagc 1440
cgtc 1444
Claims (10)
1. A cadmium-resistant lactic acid bacterium is Lactobacillus johnsonii (L.)Lactobacillus johnsonii) The preservation number is CGMCC NO. 16301.
2. The cadmium-tolerant lactic acid bacterium according to claim 1, wherein the base sequence of the 16S rRNA gene is shown in SEQ ID No. 1.
3. Cadmium-tolerant lactic acid bacterium according to claim 1 or 2, characterized in that it has the following bacteriostatic properties: has the property of inhibiting staphylococcus aureus and campylobacter jejuni, and has no property of inhibiting escherichia coli and salmonella.
4. The cadmium-tolerant lactic acid bacterium of claim 1 or 2, wherein the concentration of bile salt tolerance of the cadmium-tolerant lactic acid bacterium is 0.5%.
5. The cadmium-tolerant lactic acid bacterium according to claim 1 or 2, wherein the cadmium-tolerant lactic acid bacterium has a cadmium adsorption rate of more than 30% at a pH of 6.
6. Use of the cadmium-tolerant lactic acid bacteria of any one of claims 1 to 5 for the preparation of a cadmium adsorbent.
7. A cadmium adsorbent comprising the cadmium-tolerant lactic acid bacteria of any one of claims 1 to 5.
8. A method for adsorbing cadmium, comprising: the cadmium-tolerant lactic acid bacteria of any one of claims 1 to 5 are added to a substrate from which cadmium is to be removed.
9. A method for adsorbing cadmium as claimed in claim 8, wherein the pH of said substrate is controlled to 2.6-7.
10. A method of adsorbing cadmium as claimed in claim 9, wherein the pH of the substrate is controlled to 5.8-6.2.
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Inventor after: Pu Junhua Inventor after: Zhou Qian Inventor after: Tang Xiujun Inventor after: Zhang Jing Inventor after: Huang Shenghai Inventor after: Gao Yushi Inventor after: Chen Dawei Inventor after: Kong Lingwu Inventor after: Liu Yinyin Inventor after: Ma Lina Inventor after: Lu Junxian Inventor after: Zhang Xiaoyan Inventor after: Tang Mengjun Inventor before: Pu Junhua Inventor before: Zhou Qian Inventor before: Tang Xiujun Inventor before: Zhang Jing Inventor before: Huang Shenghai Inventor before: Kong Lingwu Inventor before: Chen Dawei Inventor before: Liu Yinyin Inventor before: Ma Lina Inventor before: Lu Junxian Inventor before: Gao Yushi Inventor before: Zhang Xiaoyan Inventor before: Tang Mengjun |