CN109197474A - A kind of method of quick screening high oil content peanut varieties - Google Patents
A kind of method of quick screening high oil content peanut varieties Download PDFInfo
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- CN109197474A CN109197474A CN201811036936.4A CN201811036936A CN109197474A CN 109197474 A CN109197474 A CN 109197474A CN 201811036936 A CN201811036936 A CN 201811036936A CN 109197474 A CN109197474 A CN 109197474A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of methods of quickly screening high oil content peanut varieties, belong to peanut breeding technical field.The method that the present invention quickly screens high oil content peanut varieties is will be under peanut seeding to be screened, drought stress processing is carried out after sowing 5 weeks, water potential value is imported in the peanut oil content of building and the relational model of the flow of water, acquires peanut oil content by the peanut leaf flow of water after measuring drought stress；Oil content is 55% the above are high oil bloom green material, and oil content is given up less than 55%.The problems such as method that the present invention quickly screens high oil content peanut varieties is simple and easy, and it is long to overcome oil content minute in conventional method, and operation sequence is complicated, expends a large amount of manpower, material resources and financial resources.In addition, the method that the present invention quickly screens high oil content peanut varieties is not limited by season and space, it is compared with the traditional method, numerical value is not much different, and the method accuracy that the present invention quickly screens high oil content peanut varieties is high, result is reliable.
Description
Technical field
The invention belongs to peanut breeding technical fields, and in particular to a kind of side of quickly screening high oil content peanut varieties
Method.
Background technique
In China, peanut is important one of oil crops, occupies an important position in agricultural and national economy.China
50% or more the peanut of production is used as oil expression, and the oil content of general peanut varieties is 50% or so, and 55% or more oil content is then
For high oil variety or high light wood material, oil content is every to improve 1 percentage point, can increase net profit 7%, therefore it is raw new to cultivate high oil bloom
Kind has become one of main breeding objective of numerous breeders.However lack high oil variety resource in peanut, lean on crossbreeding
It is difficult to obtain high oily offspring, therefore the breeding of high yield high oil variety is always an insoluble problem.The benefit of induced-mutation technique
With gene mutation or chromosomal variation can be caused, to obtain high oily mutant.But the identification of high oil mutant needs to plant
Field could be detected after maturation harvest is dried.The measurement of peanut kernel oil content at present, usually qualified unit such as
Ministry of Agriculture's oil plant and quality of item supervision and inspection center, are measured using remaining method.Measuring method very complicated, needs
The processing time is long, and the peanut for spending a large amount of manpower and material resources and financial resources, and harvesting every year can only measure an oil content.In order to
High oil body is selected to cultivate high oily new varieties, breeding process each generation will measure oil content.
Summary of the invention
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of quickly screening high oil content peanuts
The method of kind can effectively shorten breeding cycle.
In order to achieve the above object, the technical solution of the present invention is as follows:
The construction method of peanut oil content and the relational model of the flow of water, steps are as follows:
(1) multiple peanut materials of the same strain are selected, each peanut material is divided into two parts, and a part plantation is in big
Field, for measuring the seed benevolence oil content after maturation harvests, another part is planted in flowerpot, for measuring drought stress rear blade water
Gesture；
(2) measuring method of field seeding and oil content are as follows:
Sandy loam is selected, base fertilizer is used sufficient, 100 jin of the compound fertilizer that N, P, K content respectively account for 15% is applied per acre, in the first tenday period of a month in May
Sowing, depth of planting 3-4cm, covering film soil moisture conservation heat, anti-grass, during fertility pay attention to arid when water in due course, arranged in time when flooded
Water harvests pod after mature and dries, measures seed benevolence oil content.
(3) flowerpot sowing and flow of water measuring method are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and according to the fertilizer of soil
Power adds suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) to stir evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May seed is seeded in
In nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
After sowing 5 weeks carry out drought stress processing, i.e., according to the soil moisture content watering when do not water, drought stress handle 2 days；
5. beating the disk of peanut leaf after taking drought stress, the flow of water of blade is measured.
It is that y-axis constructs y=ax+b using the peanut leaf flow of water after the drought stress of measurement as x-axis, peanut kernel oil content
Relational expression.
A kind of method of quick screening high oil content peanut varieties, step are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and according to the fertilizer of soil
Power adds suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) to stir evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May are by a certain peanut
The seed that kind obtains multiple mutant through mutagenic treatment is seeded in nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
After sowing 5 weeks carry out drought stress processing, i.e., according to the soil moisture content watering when do not water, drought stress handle 2 days；
5. beating the disk of peanut leaf after taking drought stress, the flow of water of blade is measured；
6. the peanut leaf water potential value of measurement is imported in the peanut oil content of building and the relational model of the flow of water, flower is acquired
Raw oil content；
7. it is high light wood material, oil content giving up less than 55% that selection oil content, which is 55% or more mutant,.
On the basis of above scheme, the measuring method of the peanut leaf flow of water are as follows:
A, the disk of peanut leaf after taking drought stress is beaten, 10 film clips leave disks is taken to be placed in the 1mL gradient flow of water respectively
In sucrose solution, 20min is stood after shaking up；
B, the refraction coefficient of the sucrose solution after blade was impregnated in measurement, compared with raw sugar solution refraction coefficient:
If some sucrose solution refraction coefficient is identical as the refraction coefficient of raw sugar solution, measured value is the peanut
The refraction coefficient of mutant；
If the refraction coefficient of some sucrose solution is different from raw sugar solution refraction coefficient, measured one than former sugarcane
Sugar juice refraction coefficient greatly and the adjacent two kind sucrose solution that impregnated blade after smaller than raw sugar solution refraction coefficient
Refraction coefficient average be then the peanut mutant refraction coefficient；
C, the refraction coefficient of certain peanut mutant brings sucrose solution refraction coefficient into after handling measured drought stress
In the relational expression of the flow of water, the flow of water acquired is the flow of water of the peanut mutant blade.
On the basis of above scheme, the flow of water of the sucrose solution of the gradient flow of water be respectively as follows: -1.0MPa, -1.2MPa, -
1.4MPa、-1.6MPa。
On the basis of above scheme, sucrose solution refraction coefficient is the sucrose for measuring the different flows of water from the relational expression of the flow of water
The refraction coefficient of solution, and using refraction coefficient as x-axis, the relational expression of y=ax+b is constructed using the flow of water as y-axis.
The advantages of technical solution of the present invention:
1, the present invention handles the rear blade flow of water using measurement peanut drought stress, identifies the high light wood material of its peanut kernel, grasps
It is simple and easy to make method, can use manpower and material resources sparingly financial resources.Overcome in conventional method, oil content minute is long, and operation sequence is multiple
It is miscellaneous, the problems such as expending a large amount of manpower, material resources and financial resources.It is not limited by season and space.
2, the present invention handles the rear blade flow of water using measurement peanut drought stress, no matter identifies the high light wood material of peanut kernel
Which season plantation, the peanut material no matter where planted can be used as high oily expert evidence.Hainan in breeding is overcome to add
The peanut kernel oil content inaccuracy in generation, greenhouse add the seed plumpness in generation poor, can not measure material as conventional oil content
The problem of.
3, the present invention handles the rear blade flow of water using measurement peanut drought stress, identifies peanut kernel oil content, is peanut
Breeding for high oil content accelerates breeding process, shortens the breeding time limit, provides possibility.Overcome in peanut high-oil breeding, is surveyed using conventional method
Determine oil content, can only measure within 1 year primary, can not add generation, the problem of breeding year limit for length.
Detailed description of the invention
The refraction coefficient of Fig. 1 difference flow of water sucrose solution and the standard curve of the flow of water；
Fig. 2, which is spent, educates No. 20 strain peanut flow of water-oil content standard curves；
Fig. 3, which is spent, educates No. 22 strain peanut flow of water-oil content standard curves；
Fig. 4 spends Shandong No. 11 strain peanut flow of water-oil content standard curves.
Specific embodiment
Term as used in the present invention generally has those of ordinary skill in the art usual unless otherwise specified
The meaning of understanding.
Combined with specific embodiments below, and referring to the data further detailed description present invention.Following embodiment only be
It illustrates the present invention, rather than limits the scope of the invention in any way.
Embodiment 1
The construction method of peanut oil content and the relational model of the flow of water, steps are as follows:
(1) selection flower educates No. 20 respectively, flower educates No. 22 and multiple peanut materials of No. 11 strains, each peanut material are spent in Shandong
It is divided into two parts, a part plantation is in crop field, and for measuring the seed benevolence oil content after maturation harvests, another part is planted in flower
Basin, for measuring the drought stress rear blade flow of water；
(2) measuring method of field seeding and oil content are as follows:
Sandy loam is selected, base fertilizer is used sufficient, 100 jin of the compound fertilizer that N, P, K content respectively account for 15% is applied per acre, in the first tenday period of a month in May
Sowing, depth of planting 3-4cm, covering film soil moisture conservation heat, anti-grass, during fertility pay attention to arid when water in due course, arranged in time when flooded
Water harvests pod after mature and dries, and seed benevolence entrusts Ministry of Agriculture's oil plant and quality of item supervision and inspection center, utilizes remaining method
Test oil content.
(3) flowerpot sowing and flow of water measuring method are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and according to the fertilizer of soil
Power adds suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) to stir evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May seed is seeded in
In nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
After sowing 5 weeks carry out drought stress processing, i.e., according to the soil moisture content watering when do not water, drought stress handle 2 days；
5. beating the disk of peanut leaf after taking drought stress, the flow of water of blade is measured.
The measurement of the peanut leaf flow of water:
Prepare the sucrose solution of the different flows of water
According to the corresponding sucrose concentration of the known sucrose solution flow of water, the sucrose solution (table 1) of different quality concentration is prepared.
The corresponding flow of water of 1 different quality sucrose solution of table
| Sucrose (gram/100mL) | 13.34 | 15.91 | 18.44 | 20.99 |
| The flow of water (MPa) | -1.0 | -1.2 | -1.4 | -1.6 |
Make the refraction coefficient of different flow of water sucrose solutions and the standard curve of the flow of water
It measures the refraction coefficient (table 2) of each flow of water sucrose solution on Abbe refractometer, makes refraction coefficient and the flow of water
Standard curve, calibration curve formula: y=ax+b, y: the flow of water, x: the constant that refraction coefficient, a and b are automatically generated, with temperature etc.
Related, in this experiment, room temperature is 25 DEG C or so.According to refraction coefficient and the flow of water, standard curve is calculated are as follows: y=-70.367x+
93.962 coefficient R2=0.9992；
The refraction coefficient of each flow of water sucrose solution is measured on 2 Abbe refractometer of table
| The flow of water (MPa) | -1.0 | -1.2 | -1.4 | -1.6 |
| Refraction coefficient | 1.3496 | 1.3526 | 1.3552 | 1.3582 |
The refraction coefficient of the difference flow of water sucrose solution and the standard curve of the flow of water are as shown in Figure 1.
Take after 10 tracts of drought stresses respectively peanut leaf disk be dipped in respectively fill -1.0, -1.2, -1.4, -1.6MPa not
In PCR reaction tube with the 1mL sucrose solution of the flow of water；It is put into 10 disks in each pipe, closes the lid, shakes up, time of repose
It is 20 minutes.
Each sucrose solution for impregnating blade is taken respectively, and refraction coefficient is measured on Abbe refractometer.By each immersion of measurement
The sucrose solution refraction coefficient of blade, compared with raw sugar solution refraction coefficient, if some of measurement impregnates the sucrose of blade
Solution refraction coefficient is identical as the refraction coefficient of raw sugar solution, then measured value is the refraction coefficient of the kind (strain)；
If different from raw sugar solution refraction coefficient, measured one than raw sugar solution refraction coefficient greatly and one
The average of the refraction coefficient of the adjacent two kind flows of water smaller than raw sugar solution refraction coefficient is then the refractive power of the kind (strain)
Coefficient.
Through the above method, oil content, the refraction coefficient for measuring each strain peanut material are as shown in table 3, according to standard curve y
Refraction coefficient is substituted into formula, it is as shown in table 3 to obtain the flow of water by=70.367x+93.962:
Oil content, the refraction coefficient, the flow of water of each strain peanut material of table 3
It is that y-axis constructs y=ax+b using the peanut leaf flow of water after the drought stress of measurement as x-axis, peanut kernel oil content
Relational expression.As a result as shown in figs. 2 to 4:
Flower educates the standard curve of No. 20 oil contents and the flow of water are as follows: y=25.264x+90.262, related coefficient 0.882 reach
Extremely significant correlation.Flower educates the standard curve of No. 22 oil contents and the flow of water are as follows: y=14.561x+73.347, related coefficient 0.884,
Reach extremely significant correlation.Spend the standard curve of No. 11 oil contents and the flow of water in Shandong are as follows: y=22.888x+84.167, related coefficient
0.888, reach extremely significant correlation.
Embodiment 2
Spend the screening of high light wood material in No. 11 mutant in Shandong:
Select Shandong spend No. 11 mutant T77 (from60The mutant obtained in Co irradiation offspring), as test material.
The seed of T77 mature and plump is selected to plant the specific steps in flowerpot are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and according to the fertilizer of soil
Power adds suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) to stir evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May seed is seeded in
In nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
After sowing 5 weeks carry out drought stress processing, i.e., according to the soil moisture content watering when do not water, drought stress handle 2 days；
5. beating the disk of peanut leaf after taking drought stress, the flow of water measuring method measurement blade in embodiment 1 is utilized
The flow of water.
After measured, the flow of water of T77 peanut leaf is between -1.2~-1.4MPa after drought stress processing, in -1.2MPa
The refraction coefficient measured in the sucrose solution for the gesture that borders on the river with -1.4MPa this two-phase is respectively 1.3527 and 1.3531, the folding of the two
This refraction coefficient value is substituted into the refraction coefficient of different flow of water sucrose solutions and the mark of the flow of water for 1.3529 by the mean value of backscatter extinction logarithmic ratio
Directrix curve: in y=-70.367x+93.962, calculate the flow of water be -1.2375143.
The water potential value -1.2305143 of above-mentioned T77 peanut leaf is substituted into Shandong and spends No. 11 strain peanut flow of water-oil contents
Standard curve y=22.888x+84.167 show that peanut oil content is 55.84%, and the peanut kernel after field planting harvest
Ministry of Agriculture's oil plant and quality of item supervision and inspection center are entrusted, the result using remaining method test oil content is 55.78%,
The oil content of mutant T77 is greater than 55%, is high oil bloom green material；In addition, the numerical value of two methods detection is not much different, this hair
The method accuracy height of high light wood material, result are reliable in lucid and lively speed screening peanut mutant, can greatly save manpower, material resources and financial resources,
Overcome in conventional method, the long problem of oil content minute.
Embodiment 3
Flower educates the screening of high light wood material in No. 20 mutant:
Flower is selected to educate No. 20 mutant 13-5 (mutant obtained from fast neutron irradiated offspring), as test material.
The seed of 13-5 mature and plump is selected to plant the specific steps in flowerpot are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and according to the fertilizer of soil
Power adds suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) to stir evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May seed is seeded in
In nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
After sowing 5 weeks carry out drought stress processing, i.e., according to the soil moisture content watering when do not water, drought stress handle 2 days；
5. beating the disk of peanut leaf after taking drought stress, the flow of water measuring method measurement blade in embodiment 1 is utilized
The flow of water.
After measured, in the sucrose solution of -1.2MPa, refraction coefficient is 13-5 peanut leaf after drought stress processing
1.3526, identical as the refraction coefficient of raw sugar solution, therefore, the refraction coefficient of 13-5 is 1.3526.By this refraction coefficient
Value substitutes into the refraction coefficient of different flow of water sucrose solutions and the standard curve of the flow of water: in y=-70.367x+93.962, calculating
The flow of water is -1.2164042MPa.
Water potential value-the 1.2164042MPa of above-mentioned 13-5 peanut leaf is substituted into flower and educates No. 20 strain peanut flow of water-oil-containings
The standard curve y=25.26x+90.262 of rate show that peanut oil content is 59.54%, and the peanut after field planting harvest
Seed benevolence entrusts Ministry of Agriculture's oil plant and quality of item supervision and inspection center, and the result using remaining method test oil content is
59.38%, it can be seen that, mutant 13-5 oil content is greater than 55%, is high oil bloom green material；In addition, two methods detection
Numerical value is not much different, and the method accuracy that the present invention quickly screens high light wood material in peanut mutant is high, result is reliable, can be significantly
Use manpower and material resources sparingly financial resources, overcomes in conventional method, the long problem of oil content minute.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (8)
1. a kind of method of quickly screening high oil content peanut varieties, it is characterised in that: by under peanut seeding to be screened, sow
Drought stress processing is carried out after 5 weeks, water potential value is imported the peanut oil-containing of building by the peanut leaf flow of water after measuring drought stress
In the relational model of rate and the flow of water, peanut oil content is acquired；Oil content is 55% the above are high oil bloom green material, and oil content is less than
55% gives up；
The method for building up of the peanut oil content and the relational model of the flow of water are as follows:
Multiple peanut materials of the same strain are selected, each peanut material is divided into two parts, and a part plantation is used in crop field
Seed benevolence oil content after the mature harvest of measurement, another part are planted in flowerpot, for measuring the drought stress rear blade flow of water；
The pass for being y-axis building y=ax+b as x-axis, peanut kernel oil content using the peanut leaf flow of water after the drought stress of measurement
It is formula.
2. quickly screening the method for high oil content peanut varieties according to claim 1, it is characterised in that: the field seeding
With the measuring method of oil content are as follows:
Sandy loam is selected, base fertilizer is used sufficient, 100 jin of the compound fertilizer that N, P, K content respectively account for 15% is applied per acre, is sowed in the first tenday period of a month in May,
Depth of planting 3-4cm, covering film soil moisture conservation heats, anti-grass, pays attention to watering in due course when arid during fertility, drain in time when flooded, raw
Harvest pod dries after ripe, measures seed benevolence oil content.
3. quickly screening the method for high oil content peanut varieties according to claim 1, it is characterised in that: flowerpot sowing and water
Gesture measuring method are as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and is added according to the fertility of soil
Suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) stirs evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May seed is seeded in nutrition
In alms bowl, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
Drought stress processing is carried out after sowing 5 weeks；
5. beating the disk of peanut leaf after taking drought stress, the flow of water of blade is measured.
4. the method for any one quick screening high oil content peanut varieties according to claim 1~3, it is characterised in that: step
It is rapid as follows:
1. the preparation of sowing media: nutritive cube matrix selects sandy loam and vermiculite in the ratio of 4 ︰ 1, and is added according to the fertility of soil
Suitable compound fertilizer (N, 1 ︰ 1 of P, K=1 ︰) stirs evenly；
2. sowing: selecting the flowerpot of internal diameter 25cm, along maintaining an equal level in the matrix and flowerpot of loading, the first tenday period of a month in May are by a certain peanut varieties
The seed for obtaining multiple mutant through mutagenic treatment is seeded in nutritive cube, depth of planting 3-4cm；Water 600mL；
3. managing
Above-mentioned nutritive cube is put under natural environment, is watered according to soil moisture content, water 600mL every time；
4. drought stress is handled
Drought stress processing is carried out after sowing 5 weeks；
5. beating the disk of peanut leaf after taking drought stress, the flow of water of blade is measured；
6. the peanut leaf water potential value of measurement is imported in the peanut oil content of building and the relational model of the flow of water, acquires peanut and contain
Oil cut rate；
7. it is high light wood material, oil content giving up less than 55% that selection oil content, which is 55% or more mutant,.
5. quickly screening the method for high oil content peanut varieties according to claim 4, it is characterised in that: the peanut is done
Drought stress are as follows: after peanut seeding 5 weeks, or not according to soil moisture content in the watering, drought stress is handled 2 days.
6. the method for any one quick screening high oil content peanut varieties according to claim 1~3, it is characterised in that:
The measuring method of the peanut leaf flow of water after the drought stress are as follows:
A, the disk of peanut leaf after taking drought stress is beaten, 10 film clips leave disks is taken to be placed in the sucrose of the 1mL gradient flow of water respectively
In solution, 20min is stood after shaking up；
B, the refraction coefficient of the sucrose solution after blade was impregnated in measurement, compared with raw sugar solution refraction coefficient:
If some sucrose solution refraction coefficient is identical as the refraction coefficient of raw sugar solution, measured value is peanut mutation
The refraction coefficient of body；
If the refraction coefficient of some sucrose solution is different from raw sugar solution refraction coefficient, measured one more molten than raw sugar
Liquid refraction coefficient impregnated greatly the folding of the sucrose solution after blade with one adjacent two kinds smaller than raw sugar solution refraction coefficient
The average of backscatter extinction logarithmic ratio is then the refraction coefficient of the peanut mutant；
C, the refraction coefficient of certain peanut mutant brings sucrose solution refraction coefficient and water into after handling measured drought stress
In the relational expression of gesture, the flow of water acquired is the flow of water of the peanut mutant blade.
7. quickly screening the method for high oil content peanut varieties according to claim 6, it is characterised in that: the sugarcane of the gradient flow of water
The flow of water of sugar juice is respectively as follows: -1.0MPa, -1.2MPa, -1.4MPa, -1.6MPa.
8. quickly screening the method for high oil content peanut varieties according to claim 6, it is characterised in that: sucrose solution refractive power
Coefficient is the refraction coefficient for measuring the sucrose solution of the different flows of water from the relational expression of the flow of water, and using refraction coefficient as x-axis, with the flow of water
The relational expression of y=ax+b is constructed for y-axis.
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| CN108353790A (en) * | 2018-02-09 | 2018-08-03 | 青岛农业大学 | A kind of breeding method of peanut high-oil kind |
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