CN109187522A

CN109187522A - A kind of serum sodium colorimetric determination kit

Info

Publication number: CN109187522A
Application number: CN201811010736.1A
Authority: CN
Inventors: 罗维晓; 李静; 王美丽; 胡晓飞
Original assignee: Biobase Biodustry Shandong Co Ltd
Current assignee: Biobase Biodustry Shandong Co Ltd
Priority date: 2018-08-31
Filing date: 2018-08-31
Publication date: 2019-01-11

Abstract

The invention discloses a kind of sodium colorimetric determination kits, belong to clinical vitro detection reagent technique field.Kit of the present invention includes reagent R1, reagent R2, calibration object.By joined EDTA in reagent R1, thiocarbamide is added in reagent R2, influence of the bivalent cation to experiment accuracy in serum is reduced.NP-7 is added in reagent R1, keeps reaction system more uniform, to improve the sensitivity for analysis of reagent.Its range of linearity is also preferable, and the accuracy of reagent is high, is conducive to further promote the use of in the market.

Description

A kind of serum sodium colorimetric determination kit

Technical field

The present invention relates to clinical vitro detection reagent technique field, in particular to a kind of serum sodium colorimetric determination reagent Box.

Background technique

With the continuous development of ion selective electrode technology and perfect, with ion selective electrode (TSE) method to blood The analysis of middle inorganic ions is increasingly extensive, and at the same time, the other projects in laboratory measure multi-purpose spectrophotometry, for manufacture For producer and user, both automatically analyze on an instrument simultaneously and to pay higher expense and bring perhaps More technical and managerial problems.Inorganic ions in spectrophotometry measurement blood early has application, but substantially general Logical chemical method, accuracy and sensitivity are poor, and are difficult to automatically analyze.Inorganic ions can be used as in vivo The auxiliary ingredients of some enzymes play activation and amplification to enzyme reaction, and in recent years, enzymatic analysis inorganic ions achieves gratifying Progress.Such method not only has higher specificity and sensitivity, can also quickly analyze automatically, is suitable for a plurality of types of Analyzer based on spectrophotometry.

Serum sodium reduction sees the gastrointestinal tracts such as vomiting, diarrhea and loses sodium；Ephritis, nephrotic syndrome, adrenal cortex function are not Entirely, the urinary tracts such as diabetes insipidus, diabetes lose sodium；Skin loses sodium when burn, profuse sweating.Hypernatremia sees brain trauma, cerebrovascular accident, Serious dehydration, hypercortisonism etc..

Summary of the invention

In view of the problems existing in the prior art, the present invention provides a kind of sodium colorimetric determination kit, the kits Compared with conventional kit, stability and the range of linearity are got well than conventional detection kit, and sensitivity for analysis is high, favorably In the popularization and application of reagent clinically.

The present invention is achieved by the following measures:

A kind of serum sodium colorimetric determination kit, it is characterised in that, it includes reagent R1, reagent R2, wherein reagent R1 Composition are as follows: the pH 7.6Tris buffer of 100mmol/L, the beta galactosidase of 3.5KU/L, 10ml/L-30ml/L NP- 7, the EDTA of PC-300,0.05g/L-0.12g/L of kryptofix221,7.7mmol/L of 2.1mL/L；Reagent R2 composition Are as follows: 2- nitrobenzene-β-D- galactopyranoside, the 10mmol/ of the Tris buffer (pH 7.6) of 100mmol/L, 0.5g/L The BSA of the thiocarbamide of L-26mmol/L, PC-300,1g/L of 7.7mmol/L.

The ratio of the reagent R1 and reagent R2 when in use is R1:R2=120:40.

Calibration object used in the present invention is the Na calibration object of Beijing Jiuqiang Biotechnology Co., Ltd.'s production.

Kit of the invention carries out on the automatic biochemistry analyzer with double reagent function, specifically used method It is as follows:

Physiological saline, 4 μ l of sample or calibration object is added, reads suction after adding 120 μ l preincubate 5min of R1 reagent later Luminosity A1 reads absorbance A 2, and calculate Δ A after adding 5 min of reagent R2 reaction of 40 μ l later.

Beneficial effects of the present invention:

Serum sodium colorimetric determination kit provided by the invention, by the way that EDTA is added in reagent R1, in reagent R2 Thiocarbamide is added, reduces influence of the bivalent cation to experiment accuracy in serum.NP-7 is added in reagent R1, makes to react System is more uniform, to improve the sensitivity for analysis of reagent.

Detailed description of the invention

2 accuracy validation laboratory test results of Fig. 1 embodiment and control group testing result correlation；

3 accuracy validation laboratory test results of Fig. 2 embodiment and control group testing result correlation；

4 accuracy validation laboratory test results of Fig. 3 embodiment and control group testing result correlation；

Fig. 4 embodiment 1-4 linear correlation confirmatory experiment testing result；

Fig. 5 embodiment 1-4 reagent stability confirmatory experiment testing result；

Fig. 6 embodiment 1-4 reagent sensitivity confirmatory experiment testing result.

Specific embodiment

In order to better understand the present invention, it is further described combined with specific embodiments below.

Embodiment 1

A kind of serum sodium colorimetric determination kit, it includes reagent R1, reagent R2, calibration object.

Wherein reagent R1 is formed are as follows:

Reagent R2 composition are as follows:

The kit of the present embodiment description, when in use, measuring method are using the Hitachi with double reagent function 7180 automatic analyzers operate as follows:

Physiological saline, 30 μ l of sample or calibration object is added, is read after adding 200 μ l preincubate 5min of R1 reagent later Absorbance A 1 reads absorbance A 2, and calculate Δ A after adding the reagent R2 reaction 5min of 100 μ l later.

Calibration object used in the present embodiment is the NA calibration object of Beijing Jiuqiang Biotechnology Co., Ltd.'s production.

Embodiment 2

A kind of sodium colorimetric determination kit, it includes reagent R1, reagent R2, calibration object.

Wherein reagent R1 is formed are as follows:

Reagent R2 composition are as follows:

Specific measuring method is the same as embodiment 1.

Embodiment 3

Wherein reagent R1 is formed are as follows:

Reagent R2 composition are as follows:

Specific measuring method is the same as embodiment 1.

Embodiment 4

A kind of sodium colorimetric determination kit, it includes reagent R1, reagent R2, calibration object.Wherein reagent R1 is formed are as follows:

Reagent R2 composition are as follows:

Specific measuring method is the same as embodiment 1.

Experimental verification is carried out to kit assay performance obtained in above-described embodiment 1-4.

Accuracy validation experiment:

Using the kit of embodiment 2,3,4 as experimental group, a kind of excellent sodium ratio of accuracy of approval is obtained in the market Color method detection kit (production of Shenzhen company) compares experiment as a control group, detects to 40 samples, detection Result such as Fig. 1-Fig. 3.

By the detection data of Fig. 1-Fig. 3, the inspection of embodiment 2,3,4 detection kit and control test kit Surveying results relevance is respectively 0.9838,0.9920,0.9960, and correlation is relatively good, shows kit and market of the invention The upper sodium colorimetric determination kit with excellent accuracy approved that obtains has high consistency, proves kit of the present invention Other various compositions of addition will not impact its accuracy, and kit still keeps preferable accuracy.

Linear dependence confirmatory experiment:

Finding sodium high level sample is 180mmol/L, is serially diluted with physiological saline, prepares the sample of 6 various concentrations This, is followed successively by the sample of 180mmol/L, 144mmol/L, 108mmol/L, 72mmol/L, 36mmol/L, 0mmol/L concentration, Each concentration level various kinds originally measures three times respectively, takes its average value respectively.Be utilized respectively the reagent of embodiment 1,2,3,4 into Row detection.Shown in testing result Fig. 4.

Testing result shows that embodiment 1-4 testing result correlation is all larger than 0.990, but the detection of embodiment 2,3,4 As a result it is greater than 0.998, there is better linear dependence compared with Example 1, this illustrates that reagent of the present invention has better line Property correlation.

Stability confirmatory experiment:

The store reagents in 2 DEG C -8 DEG C, the light protected environment of non-corrosive gas detect the stabilization of embodiment 1-4 reagent Property.Embodiment 1-4 reagent monthly chooses same sample measures its absorbances three times, is averaged, and tries with fresh embodiment 1 Agent testing result compares, so that it is determined that the stabilization time of reagent.Detection data is shown in Fig. 5.

Experimental result shows, 1 reagent of embodiment is stored 15 months in 2 DEG C -8 DEG C, the light protected environment of non-corrosive gas Stablize, and 2,3,4 reagent of embodiment stores 24 months stabilizations in 2 DEG C -8 DEG C, the light protected environment of non-corrosive gas, explanation By joined EDTA in reagent R1, thiocarbamide is added in reagent R2, it is accurate to testing to reduce bivalent cation in serum The influence of degree.NP-7 is added in reagent R1, keeps reaction system more uniform, to improve the stability of reagent.

Sensitivity for analysis confirmatory experiment:

With serum sodium colorimetric determination kit of the present invention test known concentration in the sample of 50.3U/mL, extinction is recorded Spend difference.The reagent for being utilized respectively embodiment 1,2,3,4 is detected.Testing result is shown in Fig. 6.

By detection data, the absorbance difference of 2,3,4 detection kit of embodiment is higher than embodiment 1, says It is bright by joined EDTA in reagent R1, thiocarbamide be added in reagent R2, it is quasi- to experiment to reduce bivalent cation in serum The influence of exactness.NP-7 is added in reagent R1, keeps reaction system more uniform, to improve the sensitivity for analysis of reagent.

Therefore, serum sodium colorimetric determination kit provided by the invention is conducive to further promote in the market and make With.

Claims

1. a kind of serum sodium colorimetric determination kit, it is characterised in that, it includes reagent R1, reagent R2, wherein reagent R1 Composition are as follows: the 7.6 Tris buffer of pH of 100 mmol/L, the beta galactosidase of 3.5KU/L, 10ml/L-30ml/L The EDTA of PC-300,0.05g/L-0.12g/L of the kryptofix221 of NP-7,2.1mL/L, 7.7 mmol/L；Reagent R2 group Become: 2- nitrobenzene-β-D- galactopyranoside, the 10mmol/ of the Tris buffer (pH 7.6) of 100 mmol/L, 0.5g/L The thiocarbamide of L-26mmol/L, 7.7 mmol/L PC-300,1g/L BSA.

2. kit according to claim 1, it is characterised in that, the ratio of the reagent R1 and reagent R2 when in use For R1:R2=120:40.