CN109182175A - A kind of bacteria culture media, preparation method and application - Google Patents
A kind of bacteria culture media, preparation method and application Download PDFInfo
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- CN109182175A CN109182175A CN201811047715.7A CN201811047715A CN109182175A CN 109182175 A CN109182175 A CN 109182175A CN 201811047715 A CN201811047715 A CN 201811047715A CN 109182175 A CN109182175 A CN 109182175A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract
The present invention provides a kind of bacteria culture media, nutriment and indicator needed for containing the cariogenic bacterial growth of dental surface in the bacteria culture media, good growing environment is provided for the bacterium of dental surface, the bacterium of dental surface can be stable on the culture medium growth, the nutriment decomposed in culture medium during the growth process generates acids, the quantity of the acid of the bigger generation of the concentration of bacterium is more on culture medium, the color change of culture medium is bigger, and the content height for sampling the cariogenic bacterium of dental surface can be judged according to the color change of culture medium.The present invention facilitates the situation for understanding the cariogenic flora of dental surface in time by the way that above-mentioned culture medium is applied to the content height that the culture bacteriogenic culture medium color change of dental surface judges the cariogenic bacterium of dental surface.Bacteria Culture based component provided by the invention is simple, and each group distribution ratio is reasonable, easy to use.
Description
Technical field
The invention belongs to microorganism detection fields, and in particular to a kind of bacteria culture media, preparation method and application.
Background technique
Although people's holistic health and oral healthy condition constantly improve in recent years, mouth disease, especially dental caries are still
It is so very universal health problem.By taking children as an example: children may suffer from dental caries, with age, children from 1 years old or so
Caries prevalence rate ramp, whens 7,8 years old, peaks.Third National oral health epidemiological investigation result is shown within 2005
The 5 years old children's Primary Caries rate in China is up to 66%, there is 3.5 dental caries bad teeth per capita, occupies world's high position.12 years old dental caries caries prevalence rates
Reach 28.9%, 70% or more adult suffers from dental caries, and does not obtain medical treatment mostly.Therefore, dental caries are to influence population of China
The most important disease of oral health.Primary Caries tooth not only results in pain, influences daily life, more influence children food
The intake of object influences the growth and development of children.Dental caries are the main reasons for causing adult's sixth-year molar to lose.
Dental caries are as caused by many factors, and major influence factors are asking for bacterium, swill and tooth development
Topic.Dental caries can prevent controlling.Dental caries have specific effective precautionary measures and method, including individual forms good oral hygiene and practises
Used and feeding patterns and eating habit and doctor provide effective precautionary measures.But currently, China's most people all has
Symptom is just seen a doctor, and is only the illness rate that cannot reduce group's dental caries on the whole by the clinical treatment of doctor, is improved public
Oral health.Anticaries are better than treatment.Pre- anti-caries are be unable to do without to be detected in advance.
Summary of the invention
For the technical problem more than solving, effectively the risk of detection saprodontia illness, the present invention provide a kind of training in advance
Base is supported, contains indicator in the culture medium, the content of culture dental surface Bacteria Detection dental surface pathogenic bacteria can be passed through
Concentration level.
The object of the present invention is to provide a kind of bacteria culture medias.
It is a further object of the present invention to provide a kind of preparation methods of bacteria culture media.
Another mesh of the invention it is to provide a kind of application of above-mentioned culture medium.
In order to cultivate the bacterium of dental surface, the content of harmful bacteria in detection mouth, the present invention provides a kind of bacteria culture media,
The culture medium is the fluid nutrient medium for including coeruleum bromocresolis.
Culture medium provided by the invention is the fluid nutrient medium comprising coeruleum bromocresolis, fluid nutrient medium of the present invention
PH is 6.5-7, adds coeruleum bromocresolis in liquid medium, and coeruleum bromocresolis is crystallization that is white or being yellow, is referred to as soda acid
Show agent, pH color change interval is 3.8-5.4.Causing the bacterium of saprodontia mainly has streptococcus mutans, actinomyces, lactobacillus, due to it
Effect lead to tooth inanimate matter demineralization so that carbohydrate breakdown in the remaining food in oral cavity generates acid, cause dental caries
Tooth.Culture medium provided by the invention is seeded in above-mentioned culture medium in 37 DEG C of constant temperature incubation 48h, passes through by sampling in oral cavity
The color and standard color comparison card of culture medium compare, and obtain the content of dental surface harmful bacteria, so that it is pre- to take counter-measure to carry out
Anti- and treatment saprodontia.
Preferably, the content of the coeruleum bromocresolis is 1.0-5.5g/L.Coeruleum bromocresolis is examined in the medium as indicator
The content of bacterium is surveyed, since coeruleum bromocresolis itself is also a kind of chemical substance, when as indicator, itself is in the medium
Concentration range also will affect it is final as a result, when the concentration of coeruleum bromocresolis in the medium be this range of 1.0-5.5g/L
When, the reliability of final result is most strong.
Preferably, the culture medium further includes following components: tryptose shows, sucrose and sodium chloride, the tryptose show
Concentration in culture medium is 15-25g/L, and the concentration of the sucrose in the medium is 80-120g/L, and the sodium chloride is being cultivated
Concentration in base is 5-9g/L；It is further preferred that the culture medium further includes vitamin B and vitamin C, the vitamin B
Concentration in the medium is 0.8-1.5mg/L, and the concentration of the vitamin C in the medium is 350-550mg/；The present invention
The pH of the culture medium of offer is 6.5-7.
Tryptose in the present invention is shown as Oxiod companies market.
The preparation of culture medium is to influence the most important factor of in vitro culture microorganism effect, and determine vitro culture system
An important factor for reliability.Culture medium commonly used in the prior art contains antibiotic mostly, has effects that inhibit harmful miscellaneous bacteria,
And the good growth of bacterium is not ensured that.In order to provide the growing environment original close to bacterium, and training provided by the invention
It supports in base, by reasonably configuring the proportion of each component, provides good growing environment, the bacterium of dental surface for oral bacteria
It can normally be grown in vitro leaving primal environment, the cariogenic bacterial secretory by the constant temperature incubation of 48h, in cavity
Acid changes the color of culture medium, by the secretory volume of the change judgement acid of color, to judge the concentration of bacterium.
Preferably, every liter of the culture medium includes following components: tryptose shows 20g, sucrose 100g, sodium chloride 6g, bromine first
Phenol indigo plant 2.5g, vitamin B1 mg, vitamin C 400mg, Tween 80 5ml, surplus are water.
Preferably, every liter of the culture medium includes following components: tryptose shows 20g, sucrose 120g, sodium chloride 6g, bromine first
Phenol indigo plant 2.5g, vitamin B1 mg, vitamin C 400mg, Tween 80 6ml, surplus are water.
The preparation method of the bacteria culture media of specific embodiment according to the present invention, comprising the following steps: weigh tryptose
Show, be dissolved in water, sucrose, sodium chloride, coeruleum bromocresolis, vitamin B, vitamin C and Tween 80 is added, is uniformly mixed, in 115-
121 DEG C of progress saturated vapors sterilizing 15-30min, sterile cooling obtain the bacteria culture media.
By being sampled in oral cavity, above-mentioned bacteria culture media is seeded in 37 DEG C of constant temperature incubation dental surface bacterium 48h, is passed through
The variation for observing culture medium color, can judge the concentration of bacterium.The bacterial species of dental surface are various, and cariogenic bacterium is main
Including streptococcus mutans, actinomyces and lactobacillus.They generate acid by decomposition nutrition matter, and acid develops the color with culture medium
Reaction, judges the concentration of bacterium.
The present invention judges the content concn height of cariogenic bacteria, standard are as follows: when culture medium is by the color change of culture medium
Blue illustrates that the cariogenic bacterial content of dental surface is low, oral status health；Illustrate tooth table when culture medium color becomes blue-green
Cariogenic bacterium has had the risk for causing saprodontia in face, needs to receive professional oral care, carries out regular oral care；Work as culture
Base becomes green, and the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia is further promoted, and is treated；Work as training
Feeding base becomes yellow green and illustrates that oral cavity has already appeared saprodontia, and the risk for increasing new saprodontia is very high, needs to carry out in time
Treatment.By the color change of culture medium, it can judge the production acid amount of cariogenic bacterium in culture medium, from which further follow that bacterium
Content concn.
Bacteria culture media provided by the invention can also be used to the reagent of preparation detection dental surface bacterial content.
The invention has the benefit that
The present invention provides a kind of bacteria culture media, and the cariogenic bacterial growth institute of dental surface is contained in the bacteria culture media
The nutriment and indicator needed, provides good growing environment for the bacterium of dental surface, the bacterium of dental surface exists
Growth that can be stable on the culture medium, the nutriment decomposed in culture medium during the growth process generate acids, culture medium
The quantity of the acid of the bigger generation of the concentration of upper bacterium is more, and the color change of culture medium is bigger, according to the color change of culture medium
It can judge the content height of the sampling cariogenic bacterium of dental surface.The present invention is by being applied to culture tooth table for above-mentioned culture medium
The bacteriogenic culture medium color change in face judges the content height of the cariogenic bacterium of dental surface, helps to understand tooth table in time
The situation of the cariogenic flora in face facilitates people to take counter-measure in time, effectively avoids the generation of saprodontia.Bacterium provided by the invention
Culture medium is prepared simply, and each group distribution ratio is reasonable, easy to use.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 15g, sucrose 80g, sodium chloride 5g, coeruleum bromocresolis 1.0g, surplus is water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, be added remaining group
Point, it is uniformly mixed, in 115 DEG C of progress saturated vapors sterilizing 30min, sterile to be cooled to 40 DEG C, sterile filling obtains the culture
Base.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
Embodiment 2
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 25g, sucrose 120g, sodium chloride 9g, coeruleum bromocresolis 5.5g, surplus is water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, be added remaining group
Point, it is uniformly mixed, in 121 DEG C of progress saturated vapors sterilizing 15min, sterile to be cooled to 40 DEG C, sterile filling obtains the culture
Base.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
Embodiment 3
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 20g, sucrose 100g, sodium chloride 8g, coeruleum bromocresolis 2g, vitamin B 0.8mg, vitamin C 350mg, surplus is water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, be added remaining group
Point, it is uniformly mixed, in 121 DEG C of progress saturated vapors sterilizing 25min, sterile to be cooled to 40 DEG C, sterile filling obtains the culture
Base.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
Embodiment 4
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 20g, sucrose 100g, sodium chloride 8g, coeruleum bromocresolis 2g, vitamin B1 .5mg, vitamin C 550mg, surplus is water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, be added remaining group
Point, it is uniformly mixed, in 121 DEG C of progress saturated vapors sterilizing 25min, sterile to be cooled to 40 DEG C, sterile filling obtains the culture
Base.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
Embodiment 5
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 20g, sucrose 100g, sodium chloride 6g, coeruleum bromocresolis 2.5g, vitamin B1 mg, vitamin C 400mg, Tween 80 5ml, surplus
For water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, sucrose, chlorination is added
Sodium, coeruleum bromocresolis, vitamin B, vitamin C and Tween 80 are uniformly mixed, in 121 DEG C of progress saturated vapors sterilizing 30min, nothing
Bacterium is cooled to 30 DEG C, and sterile filling obtains the culture medium.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
Above-mentioned culture medium can be as the effective component of the reagent of the detection cariogenic bacterial content height of dental surface, for examining
Survey the content height of the cariogenic bacterium of dental surface.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
Embodiment 6
A kind of bacteria culture media, the culture medium are fluid nutrient medium, include following components: tryptose in every liter of culture medium
Show 20g, sucrose 120g, sodium chloride 6g, coeruleum bromocresolis 2.5g, vitamin B1 mg, vitamin C 400mg, Tween 80 6ml, surplus
For water.
The preparation method of above-mentioned culture medium, comprising the following steps: weigh tryptose and show, be dissolved in water, sucrose, chlorination is added
Sodium, coeruleum bromocresolis, vitamin B, vitamin C and Tween 80 are uniformly mixed, in 121 DEG C of progress saturated vapors sterilizing 30min, nothing
Bacterium is cooled to 30 DEG C, and sterile filling obtains the culture medium.
Above-mentioned culture medium culture oral bacteria first gently wipes dental surface using cotton swab and takes bacterium sample, is seeded to above-mentioned
On culture medium, in 37 DEG C of constant temperature incubation 48h, the variation of culture medium color is observed.
Above-mentioned culture medium can be as the effective component of the reagent of the detection cariogenic bacterial content height of dental surface, for examining
Survey the content height of the cariogenic bacterium of dental surface.
When culture medium be blue, illustrate that the cariogenic bacterial content of dental surface is low, oral status health；When culture medium color becomes
Illustrate that the cariogenic bacterium of dental surface has had the risk for causing saprodontia for blue-green, need to receive professional oral care, carries out just
The oral care of rule；When culture medium becomes green, the content of cariogenic bacterium is further promoted, and the risk for suffering from saprodontia further mentions
It rises, is treated；Illustrate that oral cavity has already appeared saprodontia when culture medium becomes yellow green, and increases the risk of new saprodontia
It is very high, it needs timely to be treated.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of bacteria culture media, which is characterized in that the culture medium is the fluid nutrient medium for including coeruleum bromocresolis.
2. bacteria culture media according to claim 1, which is characterized in that the content of the coeruleum bromocresolis is 1.0-5.5g/
L。
3. bacteria culture media according to claim 2, which is characterized in that the culture medium further includes following components: pancreas egg
It is white show, sucrose and sodium chloride, the tryptose shows that concentration in the medium is 15-25g/L, and the sucrose is in the medium
Concentration is 80-120g/L, and the concentration of the sodium chloride in the medium is 5-9g/L.
4. bacteria culture media according to claim 3, which is characterized in that the culture medium further includes vitamin B and Wei Sheng
Plain C, the concentration of the vitamin B in the medium are 0.8-1.5mg/L, and the concentration of the vitamin C in the medium is
350-550mg/L。
5. bacteria culture media according to claim 4, which is characterized in that every liter of the culture medium includes following components: pancreas
Proteose 20g, sucrose 100g, sodium chloride 6g, coeruleum bromocresolis 2.5g, vitamin B1 mg, vitamin C 400mg, Tween 80 5ml,
Surplus is water.
6. the preparation method of bacteria culture media described in claim 5, which comprises the following steps: weigh tryptose
Show, be dissolved in water, sucrose, sodium chloride, coeruleum bromocresolis, vitamin B, vitamin C and Tween 80 is added, is uniformly mixed, in 115-
121 DEG C of progress saturated vapors sterilizing 15-30min, sterile cooling obtain the bacteria culture media.
7. application of any bacteria culture media of claim 1-5 in detection dental surface bacterial content.
8. application according to claim 7, which is characterized in that the bacterium of the dental surface includes streptococcus mutans, is put
One of line bacterium and lactobacillus are a variety of.
9. any bacteria culture media of claim 1-5 answering in the reagent of preparation detection dental surface bacterial content
With.
10. application according to claim 9, which is characterized in that the bacterium of the dental surface includes streptococcus mutans, is put
One of line bacterium and lactobacillus are a variety of.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363777A (en) * | 2020-04-24 | 2020-07-03 | 山东爱维德生物科技有限公司 | Primary screening culture medium for increasing bacteria of dental caries pathogenic bacteria and preparation method and using method thereof |
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| CN102803504A (en) * | 2009-06-15 | 2012-11-28 | 3M创新有限公司 | Detection of acid-producing bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102803504A (en) * | 2009-06-15 | 2012-11-28 | 3M创新有限公司 | Detection of acid-producing bacteria |
| WO2012090995A1 (en) * | 2010-12-28 | 2012-07-05 | ライオン株式会社 | Method for assessing status of oral cavity, in addition to analytical device, apparatus and program therefor |
| CN103282774A (en) * | 2010-12-28 | 2013-09-04 | 狮王株式会社 | Method for assessing status of oral cavity, in addition to analytical device, apparatus and program therefor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111363777A (en) * | 2020-04-24 | 2020-07-03 | 山东爱维德生物科技有限公司 | Primary screening culture medium for increasing bacteria of dental caries pathogenic bacteria and preparation method and using method thereof |
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