CN109174788A - Microballoon cleans chip and the microballoon cleaning device comprising it - Google Patents
Microballoon cleans chip and the microballoon cleaning device comprising it Download PDFInfo
- Publication number
- CN109174788A CN109174788A CN201810944815.3A CN201810944815A CN109174788A CN 109174788 A CN109174788 A CN 109174788A CN 201810944815 A CN201810944815 A CN 201810944815A CN 109174788 A CN109174788 A CN 109174788A
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- China
- Prior art keywords
- microballoon
- cleaning
- chip
- interface
- entrance
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/041—Cleaning travelling work
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/102—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration with means for agitating the liquid
Abstract
The present invention provides a kind of microballoon cleaning chip, and the microballoon cleaning chip includes at least one wash chamber, and each wash chamber is provided at least one microballoon entrance, at least one microballoon exports and at least one is provided with the interface of microballoon blocking module.Microballoon cleaning chip of the invention and the degree of automation chemically reacted using microballoon as the different kind organism of microcarrier can be effectively promoted comprising its microballoon cleaning device, further push the development of the fields correlated response technology such as biology, chemistry, medicine, pharmacy.
Description
Technical field
The present invention relates to microfluidic arts, and in particular to a kind of microballoon cleaning chip and the microballoon cleaning dress comprising it
It sets.
Background technique
In field of biochemistry detection, microballoon is commonly used as detection carrier, carries out the biomarkers such as albumen, nucleic acid
Detection, modifies microsphere surface, so that microballoon can be with specific adsorption substance to be checked, and then completes biological marker quality testing
It surveys.It is wherein a highly important link to the cleaning of microsphere surface non-specific adsorption, directly influences subsequent detection knot
The accuracy of fruit.
Microballoon is streaming fluorescent microsphere technology as an important application of detection carrier: by each capture property reagent
It is bound to microballoon, is reacted with specific proteins, by the non-specific adsorption on multiple microballoon cleaning removal surface, is then added again
The secondary antibody for entering fluorescent marker, by detecting the average fluorescent strength of every kind of factor, thus to every kind of albumen carry out it is qualitative and
Quantitative detection.
Microballoon is high-throughput unicellular sequencing technologies as an important application of detection carrier, such as Drop-seq technology
With inDrops technology: in above two technology, being captured to from each single celled nucleic acid and modified with nucleic acid sequence
Microballoon on, the non-specific adsorption of microsphere surface could be removed by the way that microballoon is cleaned multiple times, obtain good sequencing result.
It is worth noting that, all referring to being cleaned multiple times to microballoon in above-mentioned application.Existing microballoon cleaning method is
Cleaning solution is resuspended, and-being sufficiently mixed-is centrifuged-discards supernatant, and each step is both needed to be manually operated, and needs using different instruments,
There is inconvenience in practical operation level.
To solve the above-mentioned problems, microflow control technique is a kind of suitable selection.First, stream operated by micro-fluidic chip
The volume of reaction system has good matching in body volume and above-mentioned application；Second, microflow control technique have the advantages that it is integrated,
The automation for being especially advantageous for fluid operating process is realized.Therefore, develop it is a kind of can be used for above-mentioned application microballoon cleaning chip be
The inevitable trend of one of a kind of urgent demand and biochemistry detection automatic technology.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of microballoon cleaning chip, and the microballoon cleaning chip includes at least
One wash chamber, each wash chamber are provided at least one microballoon entrance, at least one microballoon outlet and at least one
A interface for being provided with microballoon blocking module；The microballoon blocking module stops microballoon to pass through, but can pass through microballoon cleaning solution；
Apply different pressure and/or flow at each mouthful, automation drives the microballoon and microballoon cleaning solution to clean chip in microballoon
In moved by set direction, realize the microballoon cleaning and recycling of automation.
In one embodiment, microballoon cleaning chip include a wash chamber, a microballoon entrance, one it is micro-
Ball outlet is provided with the interface of microballoon blocking module with two.
In one embodiment, the microballoon blocking module includes at least one column structure, and the column structure can
To stop microballoon to pass through, but microballoon cleaning solution can be passed through.
In one embodiment, the end face of the column structure be round, rectangular, triangle, it is trapezoidal, fan-shaped or fan ring
One of shape is a variety of, it is therefore preferable to the fan annular with identical central.
In one embodiment, if the column structure is more than one, the arrangement mode of the column structure is
One of Polygonal arrays and/or annular array are a variety of, it is therefore preferable to annular array, more preferably centered on the fan
The annular array that annular end face center is overlapped.
The present invention provides a kind of microballoon cleaning device in one embodiment, and described device includes at least two above-mentioned
Microballoon cleans chip, the microballoon entrance of each microballoon cleaning chip and each pipe point being contained in the platoon pipe of microballoon to be cleaned
It does not connect, the microballoon outlet of each microballoon cleaning chip is separately connected with each pipe in the platoon pipe for recycling microballoon.
The present invention provides a kind of microballoon cleaning device in one embodiment, and described device is divided into superstructure and lower layer
Structure, the understructure include at least two above-mentioned microballoon cleaning chips, and the upper layer is connecting pipe, the connecting tube
Microballoon entrance, microballoon outlet and interface are equipped in road；The microballoon entrance of each microballoon cleaning chip of understructure is connected to
The interface of the microballoon entrance of layer structure, each microballoon cleaning chip of understructure is connected to the interface of superstructure, lower layer's knot
The microballoon outlet of each microballoon cleaning chip of structure is connected to the microballoon outlet of superstructure.
A kind of microballoon cleaning chip disclosed by the invention can realize microballoon under the driving of automation air-control device complete
It is automatically cleaned multiple times, the need of the more microsphere samples of further satisfaction and the cleaning of a large amount of microballoons is capable of comprising its microballoon cleaning device
It asks.It the described microballoon cleaning chip and can effectively be promoted comprising its microballoon cleaning device using microballoon as all kinds of of microcarrier
The degree of automation of biochemical reaction further pushes the hair of the fields correlated response technology such as biology, chemistry, medicine, pharmacy
Exhibition.
Detailed description of the invention
It in order to more clearly explain the technical solutions in the embodiments of the present application, below will be to needed in the embodiment
Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some embodiments as described in this application, right
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings
Its attached drawing.
Fig. 1 is the structural schematic diagram of a kind of single-chamber room of the present invention, four interface microballoons cleaning chip；
Fig. 2 is the structural schematic diagram of microballoon cleaning chip blocking module of the present invention；
Fig. 3 is the structural schematic diagram of a kind of single-chamber room of the present invention, six interface microballoons cleaning chip；
Fig. 4 is the structural schematic diagram of a kind of single-chamber room of the present invention, three interface microballoons cleaning chip；
Fig. 5 be include eight chambers eight samples parallel microballoon cleaning chip microballoon cleaning device schematic diagram, wherein empty
It is the schematic diagram of the microballoon cleaning device shown in line box, eight platoon pipe of microballoon sample introduction and microballoon is shown outside dashed rectangle
Recycle eight platoon pipes；With
Fig. 6 is a kind of large capacity microballoon cleaning device schematic diagram of two chambers of the invention, and wherein bold portion is described micro-
The understructure of ball cleaning device, dotted portion are the superstructure of the microballoon cleaning device.
Specific embodiment
In order to make art technology field personnel more fully understand the technical solution in the application, below in conjunction with following knot
Closing embodiment, the invention will be further described, it is clear that and described embodiments are only a part of embodiments of the present application, without
It is whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not before making creative work
All other embodiment obtained is put, shall fall within the protection scope of the present application.It is right with reference to the accompanying drawings and embodiments
The present invention is further described.
Embodiment one: a kind of single-chamber room, four interfaces microballoon clean chip
Fig. 1 is the structural schematic diagram that a kind of microballoon cleans chip.It includes a wash chamber 5 that microballoon, which cleans chip, described
Wash chamber setting there are four interface, respectively a microballoon entrance 1, a microballoon outlet 4, the setting of remaining interface 2 and 3
There is microballoon blocking module 6.
As shown in Fig. 2, microballoon blocking module 6 is one group of columnar arrays structure, end face is a series of with identical central
Fan annular, arranged in the form of annular array, the center of the annular array center annular with each fan is overlapped, to make each
Column structure has constant spacing, which is less than the diameter of microballoon, so as to stop passing through for microballoon, but can pass through
Fluid for microballoon cleaning.Microballoon blocking module 6 is designed to multilayer column array structure, be further ensured that stop microballoon can
By property.
Corresponding microballoon cleaning process includes four steps:
Step 1: microballoon sample introduction, microballoon enter wash chamber 5 by microballoon entrance 1, and cleaning solution passes through interface 2 and microballoon
Outlet 4 enters microballoon wash chamber 5, and the fluid in microballoon wash chamber 5 is discharged by interface 3.During this, microballoon can be driven
The blocking module 6 of interface 3 is moved, and is stopped by the blocking module 6.
Step 2: reverse cleaning, cleaning solution enter wash chamber 5 by microballoon entrance 1, interface 3 and microballoon outlet 4, clearly
The fluid washed in chamber 5 is discharged by interface 2.During this, microballoon can be driven to the blocking module 6 of interface 2, and be blocked by this
Module 6 is cut to stop.
Step 3: forward direction cleaning, cleaning solution enter microballoon wash chamber by microballoon entrance 1, interface 2 and microballoon outlet 4
5, the fluid in microballoon wash chamber 5 is discharged by interface 3.During this, microballoon can be driven to the blocking module 6 of interface 3,
And stopped by the blocking module 6.
Step 2 and step 3 circulation carry out five times, realize more thorough microballoon cleaning.
Step 4: microballoon recycling, microballoon cleaning solution by microballoon entrance 1, interface 2 and 3 enter wash chamber 5, microballoon with
Fluid passes through 4 discharge of microballoon outlet.In the process, microballoon can be driven to microballoon outlet 4, and then return from wash chamber 5
It receives.
It in above-mentioned steps, only need to apply different pressure at each mouthful, driving microballoon and corresponding clear can be automated
The fluids such as washing lotion are moved in microballoon cleaning chip by set direction, realize the microballoon cleaning and recycling of automation.
Embodiment two: a kind of single-chamber room, six interfaces microballoon clean chip
Fig. 3 is the structural schematic diagram that a kind of microballoon cleans chip.It includes a wash chamber 5 that microballoon, which cleans chip, described
Wash chamber setting there are six interface, respectively a microballoon entrance 1, a microballoon outlet 4, remaining four interface 2,3,
7 and 8 are provided with microballoon blocking module 6.
Corresponding microballoon cleaning process includes eight steps:
Step 1: microballoon sample introduction, microballoon enter wash chamber 5 by microballoon entrance 1, and cleaning solution passes through 2,7,8 and of interface
Microballoon outlet 4 enters microballoon wash chamber 5, and the fluid in microballoon wash chamber 5 is discharged by interface 3, and microballoon is blocked by interface 3
Module 6 is cut to stop.
Step 2: first stop cleaning, cleaning solution enter cleaning chamber by microballoon entrance 1, interface 2,3,7 and microballoon outlet 4
Room 5, the fluid in wash chamber 5 are discharged by interface 8, and microballoon is stopped by the blocking module 6 of interface 8.
Step 3: second station cleaning, cleaning solution enter cleaning chamber by microballoon entrance 1, interface 2,3,8 and microballoon outlet 4
Room 5, the fluid in wash chamber 5 are discharged by interface 7, and microballoon is stopped by the blocking module 6 of interface 7.
Step 4: the cleaning of third station, cleaning solution enter cleaning chamber by microballoon entrance 1, interface 3,7,8 and microballoon outlet 4
Room 5, the fluid in wash chamber 5 are discharged by interface 2, and microballoon is stopped by the blocking module 6 of interface 2.
Step 5: the cleaning of the 4th station, it is identical as step 3；
Step 6: the cleaning of the 5th station, it is identical as step 2；
Step 7: the cleaning of the 6th station, cleaning solution enter cleaning chamber by microballoon entrance 1, interface 2,7,8 and microballoon outlet 4
Room 5, the fluid in wash chamber 5 are discharged by interface 3, and microballoon is stopped by the blocking module 6 of interface 3.
Step 2 carries out five times to step 7 circulation, realizes more thorough microballoon cleaning.
Step 8: microballoon recycling, microballoon cleaning solution enters wash chamber 5 by microballoon entrance 1, interface 2,3,7 and 8, micro-
Ball passes through 4 discharge of microballoon outlet with fluid.
It in above-mentioned steps, only need to apply different pressure at each mouthful, driving microballoon and corresponding clear can be automated
The fluids such as washing lotion are moved in microballoon cleaning chip by set direction, realize the microballoon cleaning and recycling of automation.
Embodiment three: a kind of single-chamber room, three interfaces microballoon clean chip
Fig. 4 is the structural schematic diagram that a kind of microballoon cleans chip.It includes a wash chamber 5 that microballoon, which cleans chip, described
Wash chamber setting there are two interface, 1, the microballoon outlet 4 of a respectively microballoon entrance, remaining interface 7 is set
It is equipped with microballoon blocking module 6.
Corresponding microballoon cleaning process includes three steps:
Step 1: microballoon sample introduction, microballoon by microballoon entrance 1 enter wash chamber 5, cleaning solution by microballoon export 4 into
Enter microballoon wash chamber 5, the fluid in microballoon wash chamber 5 is discharged by interface 7, and microballoon is hindered by the blocking module 6 of interface 7
Gear.
Step 2: microballoon cleaning, cleaning solution enter wash chamber 5, wash chamber 5 by microballoon entrance 1 and microballoon outlet 4
In fluid be discharged by interface 7, microballoon is stopped by the blocking module 6 of interface 7.
Step 3: microballoon recycling, microballoon cleaning solution enter wash chamber 5 by microballoon entrance 1 and interface 7, and microballoon is with stream
Body passes through 4 discharge of microballoon outlet.
It in above-mentioned steps, only need to apply different pressure at each mouthful, driving microballoon and corresponding clear can be automated
The fluids such as washing lotion are moved in microballoon cleaning chip by set direction, realize the microballoon cleaning and recycling of automation.
A kind of example IV: microballoon cleaning device of the multiple samples of parallel processing
Part in Fig. 5 dashed rectangle is a kind of structural schematic diagram of microballoon cleaning device.The microballoon cleaning device
With eight identical units, the structure of each unit is identical as chip structure described in embodiment one.
Before microballoon cleaning device described in the present embodiment, by the microballoon entrance 11 of the 1st unit be contained with to clear
The 1st pipe 101 connection in eight platoon pipes of microballoon is washed, by the microballoon outlet 14 of the 1st unit and is used to recycle the eight of microballoon
The 1st pipe 111 connection in platoon pipe；Similarly, the microballoon entrance 81 of the 8th unit and the eight of microballoon to be cleaned will be contained with
The 8th pipe 108 connection in platoon pipe by the microballoon outlet 84 of the 8th unit and is used to recycle in eight platoon pipes of microballoon
8th pipe 118 connects, and so on.
In the cleaning process of microballoon, the microballoon in pipe 101 is driven by the microballoon entrance 11 of the 1st unit using air pressure
It moves in wash chamber 15, the microballoon in pipe 108 is driven by the microballoon entrance 81 of the 8th unit into wash chamber 85,
And so on.After microballoon in each unit microballoon cleaning process described in the embodiment one, using air pressure by the 1st list
Microballoon in 8th unit, into recovery tube 111, is passed through microballoon using air pressure by 14 driving of microballoon outlet by the microballoon in member
Outlet 84 drives into recovery tube 118, and so on.
Eight microballoon wash chambers can be used simultaneously to eight microballoons in the microballoon cleaning device through this embodiment
Sample is cleaned.
A kind of embodiment five: microballoon cleaning device of large capacity
Fig. 6 is a kind of structural schematic diagram of microballoon cleaning device.The understructure of the microballoon cleaning device has two
The structure of a identical unit, each unit is identical as chip structure described in embodiment one；The microballoon cleaning device
Superstructure be connecting pipe, the microballoon that each microballoon entrance 11 and 21 of understructure is connected to superstructure is entered respectively
Each interface 12 and 22 of understructure, is connected to the interface 122 of superstructure, by each interface of understructure by mouth 121
13 and 23 are connected to the interface 123 of superstructure, and the microballoon of understructure exports the microballoon that 14 and 24 are connected to superstructure and goes out
Mouth 124.
In the cleaning process of microballoon, microballoon is arrived into microballoon by the driving of microballoon entrance 121 of superstructure using air pressure
The superstructure of chip is cleaned, in superstructure, microballoon is assigned to the microballoon entrance 11 of two different units of understructure
With 21.After the microballoon cleaning process described in the embodiment one, microballoon is passed through into understructure using air pressure microballoon outlet 14
With 24 export, microballoon is convergeed to the microballoon outlet 124 of superstructure, is uniformly recycled.
The microballoon cleans chip through this embodiment, microballoon to be cleaned can be assigned in two wash chambers
It is cleaned parallel, to improve the capacity of cleaning.
It should be understood that the present invention disclosed is not limited only to specific method, scheme and the substance of description, because these
It is alterable.It will also be understood that purpose of the terminology used here just for the sake of the specific embodiment scheme of description, rather than
It is intended to limit the scope of the invention, the scope of the present invention is limited solely by the attached claims.
Those skilled in the art, which will also be appreciated that or be able to confirm that, uses no more than routine experiment, institute herein
The many equivalents for the specific embodiment of the invention stated.These equivalents are also contained in the attached claims.
Claims (7)
1. a kind of microballoon cleans chip, which is characterized in that the microballoon cleaning chip includes at least one wash chamber, Mei Gesuo
State wash chamber be provided at least one microballoon entrance, at least one microballoon outlet and at least one be provided with microballoon blocking module
Interface；The microballoon blocking module stops microballoon to pass through, but can pass through microballoon cleaning solution；Apply different pressures at each mouthful
Power and/or flow, automation drives the microballoon and microballoon cleaning solution to move in microballoon cleaning chip by set direction, real
The microballoon cleaning and recycling now automated.
2. microballoon according to claim 1 cleans chip, which is characterized in that the microballoon cleaning chip includes a cleaning
Chamber, a microballoon entrance, a microballoon outlet and two are provided with the interface of microballoon blocking module.
3. microballoon according to claim 1 cleans chip, which is characterized in that the microballoon blocking module includes at least one
Column structure, the column structure can stop microballoon to pass through, but can pass through microballoon cleaning solution.
4. microballoon according to claim 3 cleans chip, which is characterized in that the end face of the column structure is round, side
One of shape, triangle, trapezoidal, fan-shaped or fan annular are a variety of, it is therefore preferable to the fan annular with identical central.
5. microballoon according to claim 4 cleans chip, which is characterized in that if the column structure is more than one,
The arrangement mode of the column structure is one of Polygonal arrays and/or annular array or a variety of, it is therefore preferable to circular array
Column, more preferably centered on the annular array that is overlapped of the fan annular end face center.
6. a kind of microballoon cleaning device, which is characterized in that described device includes micro- described at least two claim 1-5 any
Ball cleans chip, and the microballoon entrance of each microballoon cleaning chip is distinguished with each pipe being contained in the platoon pipe of microballoon to be cleaned
The microballoon outlet of connection, each microballoon cleaning chip is separately connected with each pipe in the platoon pipe for recycling microballoon.
7. a kind of microballoon cleaning device, which is characterized in that described device is divided into superstructure and understructure, the understructure
Chip is cleaned including any microballoon of at least two claim 1-5, the upper layer is connecting pipe, the connecting pipe
In be equipped with microballoon entrance, microballoon outlet and interface；The microballoon entrance of each microballoon cleaning chip of understructure is connected to upper layer
The interface of the microballoon entrance of structure, each microballoon cleaning chip of understructure is connected to the interface of superstructure, understructure
Each microballoon cleaning chip microballoon outlet be connected to superstructure microballoon outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810944815.3A CN109174788B (en) | 2018-08-19 | 2018-08-19 | Microsphere cleaning chip and microsphere cleaning device comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810944815.3A CN109174788B (en) | 2018-08-19 | 2018-08-19 | Microsphere cleaning chip and microsphere cleaning device comprising same |
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| Publication Number | Publication Date |
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| CN109174788A true CN109174788A (en) | 2019-01-11 |
| CN109174788B CN109174788B (en) | 2021-07-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810944815.3A Active CN109174788B (en) | 2018-08-19 | 2018-08-19 | Microsphere cleaning chip and microsphere cleaning device comprising same |
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Cited By (1)
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| Publication number | Publication date |
|---|---|
| CN109174788B (en) | 2021-07-16 |
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