CN109160601A - Glycine betaine is alleviating the free nitrous acid of high concentration to the application in the active inhibition of anaerobic ammonium oxidation sludge - Google Patents
Glycine betaine is alleviating the free nitrous acid of high concentration to the application in the active inhibition of anaerobic ammonium oxidation sludge Download PDFInfo
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- CN109160601A CN109160601A CN201811027888.2A CN201811027888A CN109160601A CN 109160601 A CN109160601 A CN 109160601A CN 201811027888 A CN201811027888 A CN 201811027888A CN 109160601 A CN109160601 A CN 109160601A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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Abstract
The embodiment of the invention provides glycine betaines to alleviate the free nitrous acid of high concentration to the application in the active inhibition of anaerobic ammonium oxidation sludge；It is characterized in that, the high concentration is dissociated, nitrous acid range is 11~34 μ g/L.Using glycine betaine as additive, enable anaerobic ammonia oxidation process cope with high concentration dissociate nitrous acid inhibit when fast quick-recovery anaerobic ammoxidation activity, improve nitrogen removal rate (NRR) and total nitrogen (TN) removal rate.This method not only simplifies waste water processes, has saved capital expenditure and operating cost, and effectively shortens the time of anaerobic ammonium oxidation sludge domestication, has saved time cost.Meanwhile this method copes with free ammonia fluctuation with preferably stability when treating wastewater.In addition, glycine betaine low production cost, raw material are easy to get, use and not only saved processing cost, but also achieved the purpose that ecological, environmental protective.
Description
Technical field
The present invention relates to Anammox technical fields, are alleviating the free nitrous acid pair of high concentration more particularly to glycine betaine
Application in the active inhibition of anaerobic ammonium oxidation sludge.
Background technique
Anaerobic ammonium oxidation process plays an important role in nitrogen cycle, in the various anoxic ecosystems, such as ocean, underground
Water, or even Anammox phenomenon in the Antarctic Continent have been extensively studied.Compared with traditional nitration denitrification process, detest
The application of anaerobic ammonium oxidation technique reduces the demand to external source carbon source, reduces sludge yield, and operating cost is low, the technique is
It is increasingly used in the high ammonia-nitrogen wastewaters such as processing landfill leachate and municipal sewage.
The chemical equation of Anammox reaction are as follows:
NH4 ++1.32NO2 -+0.066HCO3 -+0.13H+→
1.02N2+0.26NO3 -+0.066CH2O0.5N0.15+2.03H2O chemical equation 1
Nevertheless, the application of anaerobic ammonia oxidation process is still by the complexity of actual waste water (for example, component and load
Fluctuation) limitation and substrate (ammonia nitrogen and nitrite nitrogen) and salinity etc. inhibition.Some researches show that nitrite concentration is one
Apparent inhibiting effect can be generated when more than fixed threshold value to Anammox, and the inhibition threshold value of nitrite is lower than ammonia nitrogen
Inhibit threshold value.In other words, compared with ammonia inhibition, Anammox is more vulnerable to nitrite inhibition.And newest research table
Bright, the nitrite of Anammox inhibits to be caused not instead of caused by nitrite itself by free nitrous acid (FNA)
's.Wherein, the calculation formula of FNA concentration is as follows:
Wherein, FNA is free nitrous acid concentration, [NO2 -- N] it is NO2 -- N concentration, T are temperature.
As can be seen from the above formula that FNA concentration is temperature, pH value and NO2 -The function of-N concentration three, in steady temperature
Under conditions of pH value, FNA concentration and NO2 -The concentration of-N is in positive linear dependence.
FNA is able to suppress the respiration and cell Proliferation of different microorganisms population in activated sludge.Studies have shown that FNA
The half inactivation inhibition concentration to anaerobic ammonium oxidation sludge is 11 μ g/L, and can lead to anaerobism ammonia when FNA concentration is 1.5 μ g/L
Oxidized sludge activity declines so that system unstability.When FNA concentration is reduced to 0.5 μ g/L, Anaerobic ammonium oxidation system be just able to by
Gradually restore.
Although by reducing NO in water inlet2 -The concentration and increase hydraulic detention time of-N, can to a certain extent effectively
Ground restores the nitrogen removal performance of anaerobic ammonium oxidizing bacteria, but when seriously being inhibited by FNA, anaerobic ammonium oxidizing bacteria is coped with again
The tolerance of FNA can be decreased obviously, and the anaerobic ammonium oxidation sludge as caused by high concentration FNA is active to be inhibited to be irreversible, and
This method is in project scale practice since place limitation is often difficult to realize.And by the way that gradually though domestication can be to a certain extent
Change anaerobic ammonium oxidation sludge to the tolerance for inhibiting toxicity, but often time-consuming too long, efficiency is lower.In addition, high ammonia nitrogen work
The fluctuation of industry waste water is big, more increases the difficulty that reply FNA inhibits.Therefore, how one is provided in anaerobic ammonia oxidation process
The method of kind of efficient stable, make its cope with high concentration dissociate nitrous acid inhibit anaerobic ammonium oxidation sludge activity when fast quick-recovery its
Activity becomes those skilled in the art's technical problem urgently to be resolved.
Glycine betaine is also known as betaine or trimethylglycine, chemical formula C5H11NO2, it is thin to be widely present in salt tolerant
It is a kind of effective compatible substance, while being also a kind of alkaloid in bacterium and plant, is maintaining Premeabilisation of cells pressure and alleviating salt
Stress aspect has obvious effect, therefore, can improve adaptation of the microbial flora under the adverse environments such as low temperature, arid, high salinity
Ability.Currently, glycine betaine can be by being chemically synthesized, low production cost, is suitable for large-scale production.
Summary of the invention
Inventor has found that glycine betaine can alleviate high concentration trip as additive by the research to anaerobic ammonia oxidation process
From nitrous acid to the active inhibition of anaerobic ammonium oxidation sludge；And the present invention is completed based on this.
Present invention firstly provides glycine betaines to alleviate the free nitrous acid of high concentration to the active suppression of anaerobic ammonium oxidation sludge
Application in system, wherein the range of the free nitrous acid of the high concentration is 11~34 μ g/L.
Optionally, after Anammox main body reactor start-up, the waste water of the free nitrous acid of the high concentration is conveyed
Anammox processing is carried out in Anammox main body reactor, the condition of the Anammox processing includes: anaerobism
Contain anaerobic ammonium oxidation sludge and glycine betaine in ammoxidation main body reactor.
Optionally, in Anammox main body reactor anaerobic ammonium oxidation sludge total sludge concentration be 8000~
10000mg/L。
Optionally, the concentration of glycine betaine is 1~5mmol/L in Anammox main body reactor.
Optionally, the condition of the Anammox processing further include: into giving up in Anammox main body reactor
Ammonia nitrogen (the NH of water4 +- N) concentration be 130mg/L, NH4 +- N and nitrite nitrogen (NO2 -- N) molar ratio be 1:1.32.
It should be noted that the NH in the waste water4 +- N and NO2 -- N concentration passes through addition NH respectively4Cl and NaNO2It carries out
Configuration；According to chemical equation 1 it is found that as the NH in waste water4 +- N and NO2 -When-N molar ratio is 1:1.32, detesting in reactor
Anaerobic ammonium oxidation reaction effect reaches best.
Optionally, the condition of the Anammox processing further include: control Anammox main body reactor temperature
It is 30~35 DEG C, pH value is 7.0~8.2.
Optionally, the Anammox processing includes: that the waste water of the free nitrous acid of above-mentioned high concentration is delivered to anaerobism
After in ammoxidation main body reactor, control hydraulic detention time is 2~20h.
Optionally, Anammox main body reactor starts in the following manner: by starting waste water by intake pump from
Anammox main body reactor bottom is pumped into, and control hydraulic detention time is 2~20h, and it is 1:1 that reflux pump, which controls reflux ratio,；
In the starting waste water, NH4 +- N and nitrite nitrogen (NO2 -- N) molar ratio be 1:1.32, pH value be 7.0~8.2；Anaerobism ammonia
Aoxidizing the initial sludge concentration of anaerobic ammonium oxidation sludge in main body reactor is 12000mg/L；Anammox main body reactor
Interior temperature is 30~35 DEG C；Continuous processing starting waste water, initial start stage control the NH in starting waste water4 +- N concentration is 50
~70mg/L, continuous processing NH into water outlet4 +- N and NO2 -When-N concentration is below 10mg/L, by NH4 +The concentration of-N improves
10mg/L, until NH in starting waste water4 +- N concentration is 130mg/L, controls nitrous acid concentration of dissociating in reactor and is lower than 11 μ g/
L completes the starting of Anammox main body reactor.It should be noted that the NH in starting waste water used in the present invention4 +-N
And NO2 -- N concentration passes through addition NH4Cl and NaNO2It is configured.Anaerobic ammonium oxidation sludge of the present invention can be derived from
The Anammox pilot reactor of existing operational excellence, the autotrophic type anaerobic ammonia oxidizing bacteria that anaerobic ammonium oxidation sludge contains can
To be nitrogen removal by mineralized nitrogen contained in waste water by biochemical reaction.In the present invention, the free nitrous
The concentration control of acid is to be realized based on formula 1 by adjusting pH value and temperature；The initial start stage is from for the first time to anaerobism
Ammoxidation main body reactor inputs NH4 +- N concentration is that the starting of 50~70mg/L is started with waste water, until NH4 +- N concentration is 50
The starting waste water of~70mg/L is after Anammox main body reactor for treatment, NH in water outlet4 +- N and NO2 -- N concentration is low
Until when 10mg/L.
Optionally, contain in every liter of starting waste water: CaCl2·2H2O 0.0056g, MgSO4·7H2O 0.3g, KHCO3
1.25g KH2PO40.01g, trace element solution I, II each 1mL；Wherein, in the trace element solution I, every liter contains:
EDTA 15g and FeSO45g；In the trace element solution II, every liter contains: EDTA 15g, ZnSO4·7H2O 0.43g,
CoCl2·6H2O 0.24g, MnCl2·4H2O 0.99g, CuSO4·5H2O 0.25g, Na2MoO4·2H2O 0.22g and
NiCl2·6H2O 0.19g。
Optionally, the Anammox main body reactor be arranged from top to bottom water outlet, refluxing opening, the first sample tap,
Second sample tap, third sample tap and water inlet；Heating rod and pH meter are provided in main body reactor；Main body reactor top is set
There are three separators, lye bottle and gas flowmeter；Modified poly ethylene filler is placed in main body reactor.
Glycine betaine provided in an embodiment of the present invention is active to anaerobic ammonium oxidation sludge in the free nitrous acid of alleviation high concentration
Application in inhibition, using glycine betaine as additive, so that anaerobic ammonia oxidation process is in the free nitrous acid suppression of reply high concentration
When processed can fast quick-recovery anaerobic ammoxidation activity, improve nitrogen removal rate (NRR) and total nitrogen (TN) removal rate.This method is not only
Waste water processes are simplified, capital expenditure and operating cost have been saved, and effectively shorten anaerobic ammonium oxidation sludge domestication
Time, saved time cost.Meanwhile this method copes with free ammonia fluctuation with preferably stability when treating wastewater.
In addition, glycine betaine low production cost, raw material are easy to get, use and not only saved processing cost, but also reached ecological ring
The purpose of guarantor.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is Anaerobic ammonium oxidation system schematic device；
It is indicated in figure, 1- water tank inlet；2- intake pump；3- water inlet；4- Anammox main body reactor；5- reflux
Mouthful；6- reflux pump；7- water outlet；8- goes out water tank；9- modified poly ethylene filler；Tri- separators of 10-；11- lye bottle；12-
Gas flowmeter；The first sample tap of 13-；The second sample tap of 14-；15- third sample tap；16- intelligent controller；17- heating rod；
18-pH meter.
Fig. 2 is addition glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in nitrogen removal speed
The situation of change of rate (NRR).
Fig. 3 is addition glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in total nitrogen (TN)
The situation of change of removal rate.
Fig. 4 is addition glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in tetrazolium chloride-
The situation of change of dehydrogenase activity (TTC-DHA).
Fig. 5 is addition glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in extracellular polymer
The situation of change of object (EPS).
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Firstly, being illustrated as shown in Figure 1 to Anaerobic ammonium oxidation system device used by realization various embodiments of the present invention.
Anammox main body reactor 4 and water tank inlet 1 and out water tank 8 are composed in series Anaerobic ammonium oxidation system dress
It sets, which is that cylindrical organic glass is process；Water outlet 7 is arranged from top to bottom, returns for Anammox main body reactor 4
Head piece 5, the first sample tap 13, the second sample tap 14, third sample tap 15 and water inlet 3；Water tank inlet 1 by water inlet pipe and into
Water pump 2 is connected with water inlet 3；Reflux pump 6 is connected by refluxing opening 5 with water inlet 3；Water tank 8 by outlet pipe and goes out out
The mouth of a river 7 is connected；Heating rod 17 and pH meter 18 are provided in main body reactor；Heating rod 17 is controlled by intelligent controller 16；
Main body reactor top is equipped with three separators 10, lye bottle 11 and gas flowmeter 12, and three is sequentially connected by exhaust pipe；
Modified poly ethylene filler 9 is placed in main body reactor, shape is approximately cylindrical, diameter 12mm, thickness 5mm, and density is
0.90g/cm3, filling rate is 60%~70%.
For being normally carried out for the guarantee embodiment of the present invention, need first to start Anammox main body reactor,
Purpose is the activity in order to activate anaerobic ammonia oxidizing bacteria in anaerobic ammonium oxidation sludge, and specific step is as follows for starting:
Firstly, it is inoculated with anaerobic ammonium oxidation sludge from the Anammox pilot reactor of an existing operational excellence,
And controlling its initial sludge concentration is 12000mg/L；Then, starting is anti-from Anammox main body by intake pump with waste water
Device bottom is answered to be pumped into, the starting is simulated wastewater with waste water, using distilled water as solvent, contains in every liter of starting waste water:
CaCl2·2H2O 0.0056g, MgSO4·7H2O 0.3g, KHCO31.25g KH2PO40.01g, trace element solution I, II
Each 1mL；Wherein, in the trace element solution I, every liter contains: EDTA 15g and FeSO45g；The trace element solution II
In, every liter contains: EDTA 15g, ZnSO4·7H2O 0.43g, CoCl2·6H2O 0.24g, MnCl2·4H2O 0.99g,
CuSO4·5H2O 0.25g, Na2MoO4·2H2O 0.22g and NiCl2·6H2O 0.19g.And it controls its hydraulic detention time and is
2~20h；Opening reflux pump control reflux ratio is 1:1；Water outlet flows into out water tank by water outlet.By pH meter in reactor
PH on-line monitoring, the range of pH is maintained at 7.0~8.2；Heating rod is set in reactor, is controlled by intelligent controller, is made anti-
Temperature in device is answered to be maintained at 30~35 DEG C.By adding NH4Cl and NaNO2Control water inlet substrate NH4 +- N and NO2 -- N concentration.It opens
Dynamic initial stage water inlet NH4 +The concentration control of-N is 50~70mg/L, NO2 -- N concentration is according to NH4 +- N and NO2 -- N molar ratio is 1:
1.32 prepare, and continuous processing is to being discharged NH4 +- N and NO2 -When-N concentration is below 10mg/L, by NH4 +The concentration of-N improves 10mg/
L, until NH of intaking4 +- N concentration is 130mg/L, controls FNA concentration lower than 11 by adjusting pH value and temperature based on formula 1
μ g/L, the nitric efficiency in reactor remains the trend of efficient stable, anti-by 75 days completion Anammox main bodys
Answer the starting of device.
Embodiment 1 Anammox main body reactor for treatment high concentration FNA (34 μ g/L) waste water
1.1 experimentation
Glycine betaine is added to by height for investigating using two identical Anaerobic ammonium oxidation system devices as shown in Figure 1
The influence for the Anaerobic ammonium oxidation system that concentration FNA inhibits.According to above-mentioned starting step, by two Anammox main body reactors
After starting, controlling total sludge concentration is 9000mg/L, and control hydraulic detention time is 2.5h, and water inlet is identical waste water.Its
In, glycine betaine is added in an Anammox main body reactor, and maintaining its concentration is 2mmol/L, the sample then tested
Product are denoted as RB；Another Anammox main body reactor does not add glycine betaine as a control group, the sample note then tested
For RC.The NH of waste water in two reactors4 +- N concentration is 130mg/L, NO2 -- N concentration is according to NH4 +- N and NO2 -- N molar ratio is
1:1.32 preparing；PH value is 7.0；Heating rod control reactor temperature is maintained at 30 DEG C；PH value and temperature are combined based on formula 1
FNA concentration known to value is 34 μ g/L；Total operation 140 days.
1.2 experimental result
Add glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in nitrogen removal rate
(NRR) situation of change is as shown in Figure 2；From figure 2 it can be seen that reaction starts early period, RBAnd RCIn nitrogen removal rate
(NRR) all there is significant decline；Later, as the adaptation NRR to high concentration FNA environment is gradually recovered.Wherein, RBRecovery
Time is 57 days and RCRecovery time be 117 days, the former is 2.05 times shorter than the recovery time of the latter.The result shows that glycine betaine adds
The domestication time can be obviously shortened by adding, and as a kind of relatively inexpensive compatible substance, the addition of glycine betaine has higher effect
Rate and than being more feasible by prolonged sludge acclimatization culture.
Add glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in total nitrogen (TN) removal rate
Situation of change it is as shown in Figure 3；From figure 3, it can be seen that at the 57th day, RBIn TN removal rate be 74.1%, and RCIn TN
Removal rate is 15.7%, the former is 4.72 times higher than the nitrogen removal rate of the latter.This is the result shows that the addition of glycine betaine makes anaerobism ammonia
Oxidized sludge has higher efficiency in the environment of high concentration FNA inhibits.
Add glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in tetrazolium chloride-dehydrogenation
The situation of change of enzymatic activity (TTC-DHA) is as shown in Figure 4；Figure 4, it is seen that RBAnd RCMiddle tetrazolium chloride-dehydrogenase activity
(TTC-DHA) variation tendency (see Fig. 2) of variation tendency and NRR unanimously, after 57 days, RBIn TTC-DHA be 29.4 μ
GTF/gVSS/h, and RCIn TTC-DHA be 6.2 μ gTF/gVSS/h, the former is higher by 4.7 times than the latter.Dehydrogenase is a kind of born of the same parents
Interior enzyme is closely related with intracellular oxidative phosphorylation process, is one of the important indicator for reacting bioactivity.Therefore, the knot
Fruit further demonstrates, and under high concentration FNA rejection condition, the addition of glycine betaine promotes the activity of anaerobic ammonia oxidizing bacteria.
Add glycine betaine (RB) and be not added with glycine betaine (RC) Anammox main body reactor in extracellular polymeric
(EPS) situation of change is as shown in Figure 5；From figure 5 it can be seen that the early period that reaction starts, RBAnd RCEPS content with when
Between increase and increase, then, then downward trend is presented.However, in the whole process and RBIt compares, RCMiddle sludge generates phase
To higher EPS.After 57 days, RBIn EPS (173.2mg/gVSS) than RCIn EPS (308.9mg/gVSS) low 1.8
Times.Extracellular polymeric (EPS), which refers to, to be attached to bacterium surface or is centered around around bacterium, for self-protection and is adhered to each other,
And the organic substance of carbon source and energy is provided for bacterium under adverse environment, it is mainly derived from secretion, the bacterium surface of bacterium
The falling off of substance, bacterolysis and the absorption to ambient enviroment substance are bacterium of the characterization including anaerobic ammonia oxidizing bacteria
One of active important indicator.Bacterium needs to generate a large amount of EPS to survive, originally in the environment of high concentration FNA inhibits
Applied to the energy of fixed carbon source, be used to synthesize EPS and consume, to influence bacterial growth.To a certain extent, EPS
The reduction of production quantity means the improvement of bacteria live situation.When, there are when glycine betaine, bacterium absorbs benefit from medium in environment
Bio-energy consumed by garden beet alkali is fewer than the bio-energy of synthesis EPS consumption, and therefore, the EPS synthetic quantity of bacterium is reduced,
The energy of as reply adverse environment consumption is reduced, and the energy of fixed carbon source increases, this is also that addition glycine betaine can make RBSludge
With a key factor of higher NRR in high concentration FNA environment.The result has again showed that the addition of glycine betaine alleviates
High concentration FNA environment is to the active inhibition of anaerobic ammonium oxidation sludge.
High concentration free ammonia is being alleviated to the active suppression of anaerobic ammonium oxidation sludge to glycine betaine provided by the present invention above
Application in system is described in detail.Specific embodiment used herein carries out the principle of the present invention and embodiment
It illustrates, method and its central idea of the invention that the above embodiments are only used to help understand.It should be pointed out that for this
For the those of ordinary skill in field, without departing from the principle of the present invention, several improvement can also be carried out to the present invention
And modification, these improvement and modification are also fallen into the protection scope of the claims in the present invention.
Claims (10)
1. glycine betaine is alleviating the free nitrous acid of high concentration to the application in the active inhibition of anaerobic ammonium oxidation sludge；Its feature exists
In the range of the free nitrous acid of the high concentration is 11~34 μ g/L.
2. purposes as described in claim 1, which is characterized in that after Anammox main body reactor start-up, by the height
The waste water of the free nitrous acid of concentration, which is delivered in Anammox main body reactor, carries out Anammox processing, the anaerobism ammonia
The condition of oxidation processes includes:
Contain anaerobic ammonium oxidation sludge and glycine betaine in Anammox main body reactor.
3. purposes as claimed in claim 2, which is characterized in that anaerobic ammonium oxidation sludge in Anammox main body reactor
Total sludge concentration is 8000~10000mg/L.
4. purposes as claimed in claim 2, which is characterized in that the concentration of glycine betaine is 1 in Anammox main body reactor
~5mmol/L.
5. purposes as claimed in claim 2, which is characterized in that the condition of the Anammox processing further include: enter and detest
The NH of waste water in anaerobic ammonium oxidation main body reactor4 +- N concentration is 130mg/L, NH4 +- N and NO2 -- N molar ratio is 1:1.32.
6. purposes as claimed in claim 2, which is characterized in that the condition of the Anammox processing further include: control is detested
Anaerobic ammonium oxidation main body reactor temperature is 30~35 DEG C, and pH value is 7.0~8.2.
7. purposes as claimed in claim 2, which is characterized in that the Anammox processing includes: to swim above-mentioned high concentration
After waste water from nitrous acid is delivered in Anammox main body reactor, control hydraulic detention time is 2~20h.
8. purposes as claimed in claim 2, which is characterized in that Anammox main body reactor starts in the following manner:
Starting is pumped by intake pump from Anammox main body reactor bottom with waste water, control hydraulic detention time is 2
~20h, it is 1:1 that reflux pump, which controls reflux ratio,；
In the starting waste water, NH4 +- N and NO2 -- N molar ratio is 1:1.32, and pH value is 7.0~8.2；Anammox main body
The initial sludge concentration of anaerobic ammonium oxidation sludge in reactor is 12000mg/L；Temperature in Anammox main body reactor
It is 30~35 DEG C；
Continuous processing starting waste water, initial start stage control the NH in starting waste water4 +- N concentration is 50~70mg/L, continuous to locate
Reason NH into water outlet4 +- N and NO2 -When-N concentration is below 10mg/L, by NH4 +The concentration of-N improves 10mg/L, until starting is used
NH in waste water4 +- N concentration is 130mg/L, controls nitrous acid concentration of dissociating in reactor and is lower than 11 μ g/L, completes Anammox
The starting of main body reactor.
9. purposes as claimed in claim 8, which is characterized in that contain in every liter of starting waste water: CaCl2·2H2O
0.0056g, MgSO4·7H2O 0.3g, KHCO31.25g KH2PO40.01g, trace element solution I, II each 1mL；
Wherein, in the trace element solution I, every liter contains: EDTA 15g and FeSO45g；In the trace element solution II,
Every liter contains: EDTA 15g, ZnSO4·7H2O 0.43g, CoCl2·6H2O 0.24g, MnCl2·4H2O 0.99g, CuSO4·
5H2O 0.25g, Na2MoO4·2H2O 0.22g and NiCl2·6H2O 0.19g。
10. the purposes as described in any one of claim 2-9, which is characterized in that the Anammox main body reactor is certainly
Upper and lower setting water outlet, refluxing opening, the first sample tap, the second sample tap, third sample tap and water inlet；In main body reactor
It is provided with heating rod and pH meter；Main body reactor top is equipped with three separators, lye bottle and gas flowmeter；Main body reactor
Inside it is placed with modified poly ethylene filler.
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| CN102276059A (en) * | 2011-05-24 | 2011-12-14 | 杭州师范大学 | Application of betaine for maintaining normal temperature stable operation of anammox system |
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| 阳广凤等: "厌氧氨氧化工艺的抑制现象", 《应用与环境生物学报》 * |
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