CN109160598B - Method for systematically distinguishing activity of biological membrane in sewage and wastewater treatment system - Google Patents

Method for systematically distinguishing activity of biological membrane in sewage and wastewater treatment system Download PDF

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CN109160598B
CN109160598B CN201811163094.9A CN201811163094A CN109160598B CN 109160598 B CN109160598 B CN 109160598B CN 201811163094 A CN201811163094 A CN 201811163094A CN 109160598 B CN109160598 B CN 109160598B
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biological membrane
activity
biofilm
biomembrane
content
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CN109160598A (en
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任洪强
王瑾丰
丁丽丽
许柯
张宴
刘秋菊
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Nanjing University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1236—Particular type of activated sludge installations
    • C02F3/1268—Membrane bioreactor systems
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C02F3/2853—Anaerobic digestion processes using anaerobic membrane bioreactors
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/36—Biological material, e.g. enzymes or ATP
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a method for systematically judging the activity of a biological membrane in a sewage and wastewater treatment system, belonging to the field of biological membrane sewage treatmentDomain techniques. The invention samples the biological membrane, then carries out spatial stratification to the biological membrane, and measures C in the biological membrane6‑HSL、C10‑HSL、C12Content of HS L, C in the top biofilm10When the content of the-HS L is lower than 2.2ng/g, the layer of the biological membrane is in a low activity state, and when C in the biological membrane is loosely combined12When the content of the-HS L is lower than 14ng/g, the layer of the biological membrane is in a low activity state, and when the layer of the biological membrane is tightly combined (C)6‑HSL+C10‑HSL+C12The invention analyzes the space structure of the biological membrane, and judges the activity of the biological membrane by using characteristic signal substances in the wastewater treatment biological membrane, the result is highly instructive, the judgment accuracy of the state of the sewage treatment biological membrane can be obviously improved, and the operation reliability of the biological membrane process of the sewage treatment system is improved.

Description

Method for systematically distinguishing activity of biological membrane in sewage and wastewater treatment system
Technical Field
The invention belongs to the technical field of sewage treatment by a biomembrane method, and particularly relates to a method for systematically judging the activity of a biomembrane in a wastewater treatment system.
Background
The biological membrane process is one of the mainstream processes for biological treatment of wastewater, and because the biomass of a biological membrane process system is large, the species of organisms is complex, the biological membrane process system is impact load resistant, the effluent quality is stable, and the sludge age of microorganisms is long, the biological membrane process system is favorable for the growth and the propagation of microorganisms such as nitrobacteria and the like. The biofilm on the surface of the filler can form the following stages: macromolecular adhesion, reversible adhesion, irreversible adhesion, massive extracellular polymer secretion, a biomembrane maturation stage and a biomembrane aging stage. The biological membrane can form an aerobic and anaerobic microenvironment, provides a good living environment for the denitrification and dephosphorization microorganisms, and is widely applied to the upgrading and modification of wastewater treatment plants. However, the efficiency of the biological membrane is widely affected by the activity of the biological membrane, and the biological membrane with high activity is a guarantee for the efficient and stable operation of the biological membrane process, so that the detection and judgment of the activity of the biological membrane is one of the key points of the biological membrane process for wastewater treatment.
The method for detecting the activity of the biological membrane of the sewage treatment system mainly adopts methods such as effluent quality characteristics, characteristic microorganisms, respiration rate and the like at present, and the existing method has the defects of lagging detection results, larger error and the like.
The Chinese patent application No. CN102636613A, published as 2012, 8 and 15 discloses a method for measuring the activity of an artificial wetland filler biofilm, which mainly measures the content of Dissolved Oxygen (DO) and Nitrate (NO) in unit time3 -) Sulfate radical (SO)4 2-) Ferric ion [ Fe (III)]The concentration of the artificial wetland filler reflects the activity of the biological membrane, and the method has a strong and reliable theoretical basis, but the applicability is limited to the artificial wetland filler, and the application range is small.
The Chinese patent application No. CN105203660A, published as 2015, 12 and 30 discloses a method for detecting the aging state of a biological membrane in a sewage and wastewater treatment system, which judges the aging state of the biological membrane by calculating the adenosine energy charge of the biological membrane.
Therefore, it is necessary and important to provide a method capable of comprehensively reflecting the activity of the biofilm.
Disclosure of Invention
1. Problems to be solved
Because the high-activity microorganisms are communicated with high-strength signal substances, and obvious differences exist in the biomembrane, the method for judging the activity of the biomembrane by adopting different combination modes of the content of the biomembrane characteristic signal substance AH L (N-acyl-homoserine lactone) is provided.
2. Technical scheme
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for systematically judging the activity of biological membranes in a sewage and wastewater treatment system comprises the following steps:
1) sampling a target filler biological film;
2) analyzing the target biological membrane into biological membranes in different binding forms according to the binding force, wherein the biological membranes are divided into a surface biological membrane, a loose binding biological membrane and a tight binding biological membrane;
3) extraction and determination of N-hexanoyl homoserine lactone (C) in biological membrane6-HS L), N-decanoylhomoserine cyclic lactone (C)10-HS L), N-lauroyl homoserine lactone (C)12-HS L);
4) and (3) judging the activity of different combined biological membranes by combining the biological membrane layering result: when in surface layer biological membrane C10When the content of the-HS L is lower than 2.2ng/g, the layer of the biological membrane is in a low activity state, and when C in the biological membrane is loosely combined12When the content of the-HS L is lower than 14ng/g, the layer of the biological membrane is in a low activity state, and when the layer of the biological membrane is tightly combined (C)6-HSL+C10-HSL+C12-HS L) is below 28.6ng/g, the biofilm is in a less active state;
5) the activity of the whole biological membrane is systematically judged by combining the activities of the biological membranes in different combination forms;
6) and after the effective activity of the biological membrane is obtained, judging whether the biological membrane needs to be activated.
Furthermore, the filler in the step 1) mainly aims at the annular filler which is widely used for upgrading and modifying in the current sewage and wastewater treatment plant; the biological membrane is mainly an aerobic, anaerobic and facultative biological membrane for treating industrial wastewater and municipal sewage treatment units;
furthermore, the biological membrane taken out in the step 1) is placed in a sampling box at 4 ℃, and the analysis interval time of the biological membrane is not more than 2 hours;
further, the method for analyzing the biofilm in the step 2) specifically comprises the following steps:
a. draining the water on the surface of the filler for 20-40 s;
b. selecting the diameter of the fixed pipe according to the outer diameter of the filler;
c. placing the filler into a fixed pipe, placing a single filler into a single fixed pipe, and repeating the fixed pipes by 10-15 times;
d. centrifuging for 8-12 min by a centrifugal force of 1500-2500 × g, wherein the obtained fixed tube bottom biomembrane is a surface layer combined biomembrane;
e. centrifuging for 8-12 min by using a centrifugal force of 4500-5500 × g, wherein the obtained fixed tube bottom biomembrane is a loose combination biomembrane;
f. centrifuging for 8-12 min by a centrifugal force of 9500-12000 × g to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
further, the extraction of the signal substances in the biological membrane in the step 3) is specifically as follows:
i. weighing the biological membrane, and placing the biological membrane in a glass container;
adding an organic solvent to the glass container;
sealing the glass container and placing the glass container in an ultrasonic environment;
iv, transferring the organic solvent, carrying out rotary evaporation, blowing nitrogen, and fixing the volume to 100-500 mu L by using methanol;
preferably, the weight of the biological membrane in the step i is 3-6 g;
preferably, the organic solvent in the step ii is preferably ethyl acetate, methanol or dichloromethane, and the addition volume is 30-50 m L;
preferably, the power of the ultrasound in the step iii is 100-200W, the ultrasound time is 40-80 min, the ultrasound temperature is 25-35 ℃, and the ultrasound extraction times are 3-5 times;
preferably, the rotary evaporation temperature of the organic solvent in the step iv is controlled to be 30-35 ℃, and the rotary evaporation rotating speed is 25-35 rpm;
preferably, the nitrogen blowing temperature in the step iv is controlled to be 30-35 ℃, and the nitrogen blowing flow rate is 1-3.5L/min;
furthermore, the detection method of the signal substances in the biological membrane in the step 3) is determined by adopting a high performance liquid chromatography-mass spectrometry combined method.
Further, the method for discriminating the whole biological membrane in the step 5) is as follows:
I. judging and measuring the content of AH L in the tightly bound biomembrane, comparing with the threshold value of 28.6ng/g, if the content is lower than the threshold value, judging that the activity of the whole biomembrane is low without further judgment, and if the content is higher than the threshold value, judging that the tightly bound biomembrane is in medium activity;
measuring C in the top biofilm when the content of AH L in the tightly bound biofilm is above a threshold value on the basis of I10-HS L content, compared to a threshold value of 2.2ng/g, if it is lower than the threshold value, the whole biofilm is judged to have a medium activity, without further judgment, if it is higher than the threshold value, the superficial biofilm is judged to be at a higher activity;
on the basis of II, when the content of the signal substance in the surface biofilm is higher than the threshold value, C in the loosely bound biofilm is further determined12-HS L content, compared to a threshold value of 14ng/g, if it is above the threshold value the whole biofilm is judged to have high activity, if it is below the threshold value the whole biofilm is judged to be at a higher activity.
Further, whether the whole biological membrane needs to be activated in the step 6) is judged as follows:
I. when the integral biological membrane is in high activity, the biological membrane is indicated to efficiently operate in the treatment unit without taking additional measures;
when the integral biological membrane is in high activity, the biological membrane is indicated to efficiently operate in the treatment unit, but the activity of the loosely combined biological membrane needs to be focused;
when the integral biological membrane is in the medium activity, the operation of the biological membrane in the system is stable, but necessary measures need to be taken to improve the activity of the integral biological membrane, for example, a certain proportion of biological filler is added;
and IV, when the integral biological membrane is in low activity, the operation efficiency of the biological membrane system is poor, and necessary measures need to be taken for activation.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a method for judging the activity of a biological membrane, which adopts AH L s as an indicator of the activity of the biological membrane, mainly utilizes quorum sensing behaviors existing in a wastewater treatment biological membrane to judge the activity of the biological membrane, wherein the quorum sensing behaviors among microorganisms are mutually communicated through signal molecules (such as homoserine lactone, AH L s) to coordinate the quorum behaviors, the activity of the microorganisms in a rapid growth phase is highest, and the concentration of the released signal molecules is highest at the same time, namely the concentration of the signal molecules and the activity of the biological membrane show high consistency.
(2) According to the invention, different centrifugal forces are adopted to obtain the biological membranes in different binding forms according to the internal binding force of the biological membranes, and the obtaining mode is simple, convenient and effective.
(3) The method explains the activity state of the biomembrane from the surfaces of different combination forms of biomembranes, so that the judgment of the activity state of the biomembrane is more scientific, and the refinement of subsequent regulation and control can be improved.
(4) The method reflects the activity states of different combination forms and the whole biological membrane in real time, and solves the problems of poor hysteresis and fineness of the traditional method.
(5) The method has strong pertinence to the currently widely applied standard-lifting modified filler, and provides strong guarantee for the standard-lifting modification of the sewage biomembrane method in China.
Drawings
FIG. 1 is a flow chart of the system of the present invention for discriminating the activity of different binding forms of biological membranes.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
In the embodiment, the activity detection of the biological membrane of the aerobic unit annular packing (the diameter is 2.5cm) of certain industrial wastewater is carried out, and the water quality of inlet water in the system comprises the following components of pH 7.20-7.85, chemical oxygen demand 1200-2800 mg/L, ammonia nitrogen concentration 2.85-11.54 mg/L, total nitrogen concentration 64-79 mg/L and total phosphorus concentration 12.8-28.1 mg/L.
The specific implementation process of the example is as follows:
1) sampling a target filler biofilm: placing in a 4 ℃ sampling box, and taking back to a laboratory for analysis within 2 h;
2) analyzing a target biological membrane into biological membranes with different binding forms, and specifically comprising the following steps:
a. draining the water on the surface of the filler for 30 s;
b. selecting a fixed tube with the diameter of 2.5 cm;
c. placing the filler into a fixed tube, placing a single filler into a single fixed tube, and repeating the fixed tube for 12 times;
d. centrifuging for 10min by a centrifugal force of 2000 × g to obtain a fixed tube bottom biomembrane as a surface layer combined biomembrane;
e. centrifuging for 10min by a centrifugal force of 5000 × g to obtain a fixed tube bottom biomembrane which is loosely combined with the biomembrane;
f. centrifuging for 10min by 10000 × g centrifugal force to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
3) the method for extracting the signal substances in the biological membrane comprises the following specific steps:
i. weighing 5g of biological membrane, and placing the biological membrane in a glass container;
ii, adding 30m L ethyl acetate agent into a glass container;
iii, sealing the glass container, and placing the glass container in an ultrasonic environment, wherein the ultrasonic power is 100W, the ultrasonic time is 40min, the ultrasonic temperature is 30 ℃, and the extraction times are 3 times;
transferring the organic solvent, carrying out rotary evaporation at 30 ℃ and 30rpm, carrying out nitrogen blowing at 30 ℃ and a nitrogen blowing flow rate of 1L/min, and fixing the volume to 500 mu L by using methanol;
4) adopting high performance liquid chromatography-mass spectrometry to measure signal substances in the biological membrane: c in surface layer biofilm10The content of-HS L is 1.8ng/g, and C in the loose-binding biological membrane12The content of-HS L is 13.8ng/g, and the material is tightly combined in the biological membrane (C)6-HSL+C10-HSL+C12HS L) is 22.3ng/g, the content of signal substances in the biological membranes of the three different binding forms is lower than a threshold value;
5) the integral biological membrane discrimination method specifically comprises the following steps:
the tightly bound biofilm is in a low activity state, indicating that the overall biofilm is in a low activity state and may require activation.
Example 2
In the embodiment, the activity detection of the biofilm of the aerobic unit annular filler (the diameter is 2.5cm) of certain domestic sewage is carried out, and the quality of inlet water in the system comprises the following components of pH 7.0-7.55, chemical oxygen demand 220-380 mg/L, ammonia nitrogen concentration 38.5-61.54 mg/L, total nitrogen concentration 67.2-79 mg/L and total phosphorus concentration 3.8-8.1 mg/L.
The specific implementation process of the example is as follows:
1) sampling a target filler biofilm: placing in a 4 ℃ sampling box, and taking back to a laboratory for analysis within 2 h;
2) analyzing a target biological membrane into biological membranes with different binding forms, and specifically comprising the following steps:
a. draining the water on the surface of the filler for 40 s;
b. selecting a fixed tube with the diameter of 2.5 cm;
c. placing the filler into a fixed tube, placing a single filler into a single fixed tube, and repeating the fixed tubes by 15;
d. centrifuging for 8min by a centrifugal force of 2000 × g to obtain a fixed tube bottom biomembrane as a surface layer combined biomembrane;
e. centrifuging for 8min by a centrifugal force of 5000 × g to obtain a fixed tube bottom biomembrane which is loosely combined with the biomembrane;
f. centrifuging for 8min by 10000 × g centrifugal force to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
3) the method for extracting the signal substances in the biological membrane comprises the following specific steps:
i. weighing 3g of biological membrane, and placing the biological membrane in a glass container;
ii, adding 30m L ethyl acetate agent into a glass container;
iii, sealing the glass container, and placing the glass container in an ultrasonic environment, wherein the ultrasonic power is 100W, the ultrasonic time is 40min, the ultrasonic temperature is 30 ℃, and the extraction times are 3 times;
transferring the organic solvent, carrying out rotary evaporation at 30 ℃ and 30rpm, carrying out nitrogen blowing at 30 ℃ and a nitrogen blowing flow rate of 1L/min, and fixing the volume to 200 mu L by using methanol;
4) adopting high performance liquid chromatography-mass spectrometry to measure signal substances in the biological membrane: c in surface layer biofilm10The content of-HS L is 0.9ng/g, and C in the loose-binding biological membrane12The content of-HS L is 18.8ng/g, and the material is tightly combined in the biological membrane (C)6-HSL+C10-HSL+C12HS L) in the biofilm, the content of the signal substances in the surface layer biofilm is lower than a threshold value, and the threshold values of the signal substances in the loosely-bound and tightly-bound biofilms are higher than the threshold value;
5) the integral biological membrane discrimination method specifically comprises the following steps:
and if the content of the tightly bound biomembrane signal substances is higher than the threshold value and the content of the surface biomembrane signal substances is lower than the threshold value, judging that the whole biomembrane is in medium activity.
Example 3
In the embodiment, the activity detection of the biomembrane of the unit annular filler (with the diameter of 2.5cm) of aerobic waste water in a certain park is carried out, and the water quality of inlet water in the system comprises the pH value of 7.30-8.05, the chemical oxygen demand of 2200-3400 mg/L, the ammonia nitrogen concentration of 12.85-32.54 mg/L, the total nitrogen concentration of 84-99 mg/L and the total phosphorus concentration of 22.8-38.1 mg/L.
The specific implementation process of the example is as follows:
1) sampling a target filler biofilm: placing in a 4 ℃ sampling box, and taking back to a laboratory for analysis within 2 h;
2) analyzing the target biological membrane into biological membranes with different binding forms, and specifically comprising the following steps:
a. draining the water on the surface of the filler for 30 s;
b. selecting a fixed tube with the diameter of 2.5 cm;
c. placing the filler into a fixed tube, placing a single filler into a single fixed tube, and repeating the fixed tube for 12 times;
d. centrifuging for 12min by a centrifugal force of 2000 × g to obtain a fixed tube bottom biomembrane as a surface layer combined biomembrane;
e. centrifuging for 12min by a centrifugal force of 5000 × g to obtain a fixed tube bottom biomembrane which is loosely combined with the biomembrane;
f. centrifuging for 12min by 10000 × g centrifugal force to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
3) the method for extracting the signal substances in the biological membrane comprises the following specific steps:
i. weighing 3g of biological membrane, and placing the biological membrane in a glass container;
ii, adding 30m L ethyl acetate agent into a glass container;
iii, sealing the glass container, and placing the glass container in an ultrasonic environment, wherein the ultrasonic power is 100W, the ultrasonic time is 40min, the ultrasonic temperature is 30 ℃, and the extraction times are 3 times;
transferring the organic solvent, carrying out rotary evaporation at 30 ℃ and 30rpm, carrying out nitrogen blowing at 30 ℃ and a nitrogen blowing flow rate of 1L/min, and fixing the volume to 500 mu L by using methanol;
4) adopting high performance liquid chromatography-mass spectrometry to measure signal substances in the biological membrane: c in surface layer biofilm10The content of-HS L is 11.8ng/g, and C in the loosely bound biological membrane12The content of-HS L is 18.9ng/g, and the material is tightly combined in the biological membrane (C)6-HSL+C10-HSL+C12HS L) is 32.7ng/g, the content of signal substances in the biological membranes of the three different binding forms is higher than a threshold value;
5) the integral biological membrane discrimination method specifically comprises the following steps:
and if the content of the signal substances in the three types of biological membranes in different combination forms is higher than a threshold value, judging that the whole biological membrane is in a high-activity state.
Example 4
In the embodiment, the activity detection of the biofilm of the aerobic unit annular filler (the diameter is 2.5cm) of certain domestic sewage is carried out, and the quality of inlet water in the system comprises the following components of pH 7.0-7.55, chemical oxygen demand 220-380 mg/L, ammonia nitrogen concentration 38.5-61.54 mg/L, total nitrogen concentration 67.2-79 mg/L and total phosphorus concentration 3.8-8.1 mg/L.
The specific implementation process of the example is as follows:
1) sampling a target filler biofilm: placing in a 4 ℃ sampling box, and taking back to a laboratory for analysis within 2 h;
2) analyzing a target biological membrane into biological membranes with different binding forms, and specifically comprising the following steps:
a. draining the water on the surface of the filler for 40 s;
b. selecting a fixed tube with the diameter of 2.5 cm;
c. placing the filler into a fixed tube, placing a single filler into a single fixed tube, and repeating the fixed tubes by 15;
d. centrifuging for 8min by 1500 × g centrifugal force to obtain fixed tube bottom biomembrane as surface layer combined biomembrane;
e. centrifuging for 8min by 4500 × g centrifugal force to obtain fixed tube bottom biomembrane;
f. centrifuging for 8min by a centrifugal force of 9500 × g to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
3) the method for extracting the signal substances in the biological membrane comprises the following specific steps:
i. weighing 3g of biological membrane, and placing the biological membrane in a glass container;
add 50m L methanol to a glass vessel;
sealing the glass container, and placing the glass container in an ultrasonic environment, wherein the ultrasonic power is 150W, the ultrasonic time is 60min, the ultrasonic temperature is 25 ℃, and the extraction times are 4 times;
transferring the organic solvent, carrying out rotary evaporation at 33 ℃, 25rpm, carrying out nitrogen blowing at 33 ℃ and the nitrogen blowing flow rate of 17L/min, and fixing the volume to 100 mu L by adopting methanol;
4) adopting high performance liquid chromatography-mass spectrometry to measure signal substances in the biological membrane: c in surface layer biofilm10Content of-HS L of 12ng/g, loosely bound in biofilm C12The content of-HS L is 11.8ng/g, and the material is tightly combined in the biological membrane (C)6-HSL+C10-HSL+C12HS L) is 18.3ng/g, the content of signal substances in the biological membranes of the three different binding forms is lower than a threshold value;
5) the integral biological membrane discrimination method specifically comprises the following steps:
and if the content of the tightly bound biological membrane signal substances is lower than a threshold value, judging that the whole biological membrane is in a low activity state and possibly needing to be activated.
Example 5
In the embodiment, the activity detection of the biomembrane of the unit annular filler (with the diameter of 2.5cm) of aerobic waste water in a certain park is carried out, and the water quality of inlet water in the system comprises the pH value of 7.30-8.05, the chemical oxygen demand of 2200-3400 mg/L, the ammonia nitrogen concentration of 12.85-32.54 mg/L, the total nitrogen concentration of 84-99 mg/L and the total phosphorus concentration of 22.8-38.1 mg/L.
The specific implementation process of the example is as follows:
1) sampling a target filler biofilm: placing in a 4 ℃ sampling box, and taking back to a laboratory for analysis within 2 h;
2) analyzing a target biological membrane into biological membranes with different binding forms, and specifically comprising the following steps:
a. draining the water on the surface of the filler for 20 s;
b. selecting a fixed tube with the diameter of 2.5 cm;
c. placing the filler into a fixed tube, placing a single filler into a single fixed tube, and repeating the fixed tube by 10 times;
d. centrifuging for 12min by a centrifugal force of 2500 × g, wherein the obtained fixed tube bottom biomembrane is a surface layer combined biomembrane;
e. centrifuging for 12min by 5500 × g centrifugal force to obtain a fixed tube bottom biomembrane which is loosely combined with the biomembrane;
f. centrifuging for 12min by a centrifugal force of 12000 × g to obtain a fixed tube bottom biomembrane which is a tightly combined biomembrane;
3) the method for extracting the signal substances in the biological membrane comprises the following specific steps:
i. weighing 6g of biological membrane, and placing the biological membrane in a glass container;
add 40m L dichloromethane to the glass vessel;
sealing the glass container, and placing the glass container in an ultrasonic environment, wherein the ultrasonic power is 200W, the ultrasonic time is 80min, the ultrasonic temperature is 35 ℃, and the extraction times are 5 times;
transferring the organic solvent, carrying out rotary evaporation at 35 ℃ and 35rpm, carrying out nitrogen blowing at 35 ℃ and 35 ℃ at a nitrogen blowing flow rate of 35L/min, and fixing the volume to 300 mu L by adopting methanol;
4) adopting high performance liquid chromatography-mass spectrometry to measure signal substances in the biological membrane: c in surface layer biofilm10The content of-HS L is 21.3ng/g, and C in the loose-binding biological membrane12The content of-HS L is 28.9ng/g, and the material is tightly combined in the biological membrane (C)6-HSL+C10-HSL+C12HS L) is 68.7ng/g, the content of signal substances in the biological membranes of the three different binding forms is higher than a threshold value;
5) the integral biological membrane discrimination method specifically comprises the following steps:
and if the content of the signal substances in the three types of biological membranes in different combination forms is higher than a threshold value, judging that the whole biological membrane is in a high-activity state.
It is to be understood that variations may be derived or suggested from the present disclosure and general knowledge to those skilled in the art in light of the present disclosure and detailed description. Those skilled in the art will appreciate that other methods, alternatives to those known in the art, and insubstantial changes in the combination of features from one another, which are known in the art, can be used, and are intended to be within the scope of the invention.

Claims (9)

1. A method for systematically discriminating the activity of biofilms in a wastewater treatment system, comprising the steps of:
(1) sampling a target filler biological film;
(2) analyzing the target biological membrane into biological membranes in different binding forms according to the binding force, wherein the biological membranes are divided into a surface biological membrane, a loose binding biological membrane and a tight binding biological membrane;
(3) extracting and measuring the content of signal substances in the biological membrane, wherein the signal substances comprise: n-hexanoyl homoserine lactone (C)6-HS L), N-decanoylhomoserine cyclic lactone (C)10-HS L), N-lauroyl homoserine lactone (C)12-HSL)；
(4) And (3) judging the activity of the biological membranes in different binding forms by combining the biological membrane layering result: when in surface layer biological membrane C10Judging that the biofilm is in a low activity state when the content of-HS L is less than 2.2ng/g, and judging that the biofilm is in a low activity state when C in the loosely bound biofilm is12When the content of-HS L is lower than 14ng/g, the layer of biological membrane is judged to be in a low activity state, and when the layer of biological membrane is tightly combined (C)6-HSL+C10-HSL+C12-HS L) is lower than 28.6ng/g, the biofilm is judged to be in a low activity state;
(5) combining the activities of the biological membranes in different combination forms, systematically judging the activity of the whole biological membrane, wherein the judging method specifically comprises the following steps:
I. judging and measuring the content of AH L in the tightly bound biomembrane, comparing with the threshold value of 28.6ng/g, if the content is lower than the threshold value, judging that the activity of the whole biomembrane is low without further judgment, and if the content is higher than the threshold value, judging that the tightly bound biomembrane is in medium activity;
measuring C in the top biofilm when the content of AH L in the tightly bound biofilm is above a threshold value on the basis of I10-HS L content, compared to a threshold value of 2.2ng/g, if it is lower than the threshold value, the whole biofilm is judged to have a medium activity, without further judgment, if it is higher than the threshold value, the superficial biofilm is judged to be at a higher activity;
on the basis of II, when the content of the signal substance in the surface biofilm is higher than the threshold value, C in the loosely bound biofilm is further determined12-HS L content, compared to a threshold value of 14ng/g, if it is above the threshold value the whole biofilm is judged to have high activity, if it is below the threshold value the whole biofilm is judged to be at a higher activity.
2. The method of claim 1 for a system for determining biofilm activity in a wastewater treatment system, comprising: the filler in the step (1) mainly aims at the annular filler which is widely used for upgrading and modifying in the current sewage and wastewater treatment plant; the biological membrane is mainly an aerobic, anaerobic and facultative biological membrane for treating industrial wastewater and municipal sewage treatment units.
3. The method of claim 1 for a system for determining biofilm activity in a wastewater treatment system, comprising: and (2) placing the biological membrane taken out in the step (1) in a 4 ℃ sampling box, wherein the analysis interval time of the biological membrane is not more than 2 hours.
4. The method of claim 1 for a system for determining biofilm activity in a wastewater treatment system, comprising: the method for analyzing the biological membrane in the step (2) comprises the following specific steps:
a. draining the water on the surface of the filler for 20-40 s;
b. selecting the diameter of the fixed pipe according to the outer diameter of the filler;
c. placing the filler into a fixed pipe, placing a single filler into a single fixed pipe, and repeating the fixed pipes by 10-15 times;
d. centrifuging for 8-12 min by a centrifugal force of 1500-2500 × g, wherein the obtained fixed tube bottom biomembrane is a surface layer combined biomembrane;
e. centrifuging for 8-12 min by using a centrifugal force of 4500-5500 × g, wherein the obtained fixed tube bottom biomembrane is a loose combination biomembrane;
f. centrifuging for 8-12 min by a centrifugal force of 9500-12000 × g, and obtaining the fixed tube bottom biomembrane which is tightly combined with the biomembrane.
5. The method of claim 1 for a system for determining biofilm activity in a wastewater treatment system, comprising: the extraction of the signal substances in the biological membrane in the step (3) is specifically as follows:
i. weighing the biological membrane, and placing the biological membrane in a glass container;
adding an organic solvent to the glass container;
sealing the glass container and placing the glass container in an ultrasonic environment;
and iv, transferring the organic solvent, performing rotary evaporation, blowing nitrogen, and metering the volume to 100-500 mu L by using methanol.
6. The method of claim 5 for a system for determining biofilm activity in a wastewater treatment system, comprising: the weight of the biological membrane in the step i is 3-6 g, and the organic solvent in the step ii is preferably ethyl acetate, methanol or dichloromethane.
7. The method of claim 5 for a system for determining biofilm activity in a wastewater treatment system, comprising: in the step iii, the power of ultrasound is 100-200W, the ultrasound time is 40-80 min, the ultrasound temperature is 25-35 ℃, and the ultrasound extraction times are 3-5.
8. The method for systematically judging the activity of a biofilm in a sewage and wastewater treatment system according to claim 5, wherein the rotary evaporation control temperature of the organic solvent in the step iv is 30-35 ℃, the rotary evaporation rotation speed is 25-35 rpm, the nitrogen blowing control temperature is 30-35 ℃, and the nitrogen blowing flow rate is 1-35L/min.
9. The method of claim 5 for a system for determining biofilm activity in a wastewater treatment system, comprising: and (4) determining the detection method of the signal substances in the biological membrane in the step (3) by adopting a high performance liquid chromatography-mass spectrometry combined method.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248221A (en) * 1992-10-22 1993-09-28 Rohm And Haas Company Antifouling coating composition comprising lactone compounds, method for protecting aquatic structures, and articles protected against fouling organisms
CN105084552A (en) * 2015-07-31 2015-11-25 北京师范大学 Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248221A (en) * 1992-10-22 1993-09-28 Rohm And Haas Company Antifouling coating composition comprising lactone compounds, method for protecting aquatic structures, and articles protected against fouling organisms
CN105084552A (en) * 2015-07-31 2015-11-25 北京师范大学 Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones

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