CN109156354A - A kind of growth regulator inducing indigo fruit Callus of Leaf - Google Patents
A kind of growth regulator inducing indigo fruit Callus of Leaf Download PDFInfo
- Publication number
- CN109156354A CN109156354A CN201811178201.5A CN201811178201A CN109156354A CN 109156354 A CN109156354 A CN 109156354A CN 201811178201 A CN201811178201 A CN 201811178201A CN 109156354 A CN109156354 A CN 109156354A
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- growth regulator
- callus
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- plant
- leaf
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of growth regulator inducing indigo fruit Callus of Leaf belongs to field of biotechnology, method includes the following steps: plant is isolated and suited the requirements.Organ is or, protoplast etc. is cultivated under manual control condition by sterile working to obtain the technology of other products of regenerated intact plant or production with economic value.Plant concept refers to plant parts tissue, such as mesophyll tissue, carries out culture and obtain regeneration plant, also refers to the culture generated from each organ during the cultivation process, and callus is using forming aftergrowth.An important technology of the current organization culture as bioengineering, the effect played in fundamental research and production practices are growing day by day, it is expected to make contributions to promote the well-being of mankind.
Description
Technical field
The invention belongs to field of biotechnology, relate generally to single plant growth regulator induction indigo fruit blade callus group
The method knitted.
Background technique
Currently, research mainly includes two aspects in terms of blue fruit honeysuckle tissue cultures and Regeneration in Vitro: first is that with stem with bud,
Axillary bud, stem apex etc. are that explant establishes tissue culture rapid propagation system, explore each cultivation stage and are suitble to growth regulator ratio, suitable basic
Culture medium, condition of culture etc..Second is that establishing regenerating system with stem section and blade evoked callus.
Blue fruit honeysuckle kind is relatively single in actual production, lacks high quality seedling, it is difficult to meet the market demand, improve breeding
Efficiency, cultivating has different characteristics new varieties, and it is particularly necessary to establish blue fruit honeysuckle tissue culture rapid propagation system.It is with Regeneration in Vitro technology
Breeding efficiency can be improved in the genetic transfoumation on basis, widens breeding methods, accelerates seedling-wood breeding.Callus induction is to establish
Vitro Regeneration System steps necessary, and study blue fruit honeysuckle active constituent basis.
During tissue-culturing rapid propagation and in vitro plant regeneration, plant growth regulator is played an important role, often through
The differentiation rate of tissue-culturing rapid propagation process can be made by applying different plant growth regulator or the simple concentration for changing growth regulator
It is greatly increased with growth coefficient, and growth regulator is even more to have very important key work during in vitro plant regeneration
With plant hormone and plant growth regulator are living by the intracorporal various signal system controlling genes of plant as first messenger
Property, the expression of related gene is controlled, and then promote or inhibit the generation of isolated organ, different growth regulators lure callus
It leads and the effectiveness of neomorph is different, the function and effect of same growth regulator various concentration are also different.Therefore research plant is raw
Effect of long regulator during tissue-culturing rapid propagation and in vitro plant regeneration is to establish tissue culture rapid propagation system and in vitro plant regeneration
The importance of system.Callus induction is the steps necessary for establishing vitro Regeneration System, and the induction of callus is also deep
Enter the basis for studying blue fruit honeysuckle active constituent.By callus to can also need in the intact plant that market utilizes by callus
Tissue breaks up the multinomial link such as adventitious bud and culture of rootage, acclimatization and transplants again.To blue fruit honeysuckle tissue culture and vitro Regeneration System
Action rule, reference can be provided for research from now on, for indigo plant fruit honeysuckle industry development provide theory and technology support.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a kind of single plant growth regulating
The method of agent inducer blade callus, reaches the process of fast and effective Propagated Plantlets.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions:
1, the method for single plant growth regulator induction indigo fruit Callus of Leaf, which is characterized in that this method packet
Include following steps:
(1) taking two quoted from Russian table variety Qiu rem and Na rem is test material；
(2) dark culture condition is humidity 75%, temperature (25 ± 1) DEG C.Dark culture condition be humidity 75%, temperature (25 ±
1)℃；
(3) different MS culture medium is configured, squamous subculture 30d takes aseptic seedling middle and upper part blade, in face of blade when inoculation
Crosscutting formation scratch, vacuum side of blade contact culture medium；
(4) it is inoculated into addition sucrose, agar solution, adds different growth regulator MS culture mediums, it is dark in phjytotron
It cultivates to be transferred under light after the corresponding time and cultivate, cultivate a couple of days.
2, the method for single plant growth regulator induction Callus of Leaf according to claim 1, feature
It is, two kinds described in step (1) pick up from Northeast Agricultural University's gardening experiment centre small berries Germplasm Resources.
3. the method for single plant growth regulator induction Callus of Leaf according to claim 1, feature
It is, MS culture medium described in step (3) is respectively 6-BA, 2,5 kinds of hormones of 4-D, TDZ, ZT and CPPU, concentration is 0.5,
1.0、2.0mg·L-1。
4. the method for single plant growth regulator induction Callus of Leaf according to claim 1, feature
It is, sucrose concentration described in step (4) is 30gL-1, agar concentration 7gL-1, it is desirable that it is pH 5.8, cultivates 40 days
Left and right.
The method of single plant growth regulator induction indigo fruit Callus of Leaf belongs to field of biotechnology, this method
The following steps are included: plant isolates the tissue to suit the requirements.Organ or cell, protoplast etc., by sterile working,
It is cultivated under manual control condition to obtain the technology of other products of regenerated intact plant or production with economic value.
Plant Tissue Breeding concept refers to plant parts tissue, such as forming layer, parenchymal tissue, mesophyll tissue, endosperm are cultivated
Regeneration plant is obtained, also refers to the culture for generating callus from each organ during the cultivation process, callus using dividing again
Change forms aftergrowth.An important technology of the current organization culture as bioengineering, it is real in fundamental research and production
The effect for trampling middle performance is growing day by day, it is expected to make contributions to promote the well-being of mankind.
Detailed description of the invention
Attached drawing 1 is this processing technology routine figure.
Specific embodiment
Embodiment 1:
(1) taking two quoted from Russian table variety Qiu rem and Na rem is test material；
(2) dark culture condition is humidity 75%, temperature (25 ± 1) DEG C.Dark culture condition be humidity 75%, temperature (25 ±
1)℃；(3) different MS culture medium is configured, squamous subculture 30d takes aseptic seedling middle and upper part blade, in face of blade cross when inoculation
It cuts to form scratch, vacuum side of blade contacts culture medium；
(4) it is inoculated into addition sucrose, agar solution, adds different growth regulator MS culture mediums, it is dark in phjytotron
It cultivates to be transferred under light after the corresponding time and cultivate, cultivate a couple of days.
Embodiment 2:
(1) taking two quoted from Russian table variety Qiu rem and Na rem is test material；
(2) dark culture condition is humidity 75%, temperature (25 ± 1) DEG C.Dark culture condition be humidity 75%, temperature (25 ±
1)℃；
(3) different MS culture medium is configured, squamous subculture 40d takes aseptic seedling middle and upper part blade, in face of blade when inoculation
Crosscutting formation scratch, vacuum side of blade contact culture medium；
(4) it is inoculated into addition sucrose, agar solution, adds different growth regulator MS culture mediums, it is dark in phjytotron
It cultivates to be transferred under light after the corresponding time and cultivate, cultivate a couple of days.
Embodiment 3:
(1) taking two quoted from Russian table variety Qiu rem and Na rem is test material；
(2) dark culture condition is humidity 75%, temperature (25 ± 1) DEG C.Dark culture condition be humidity 75%, temperature (25 ±
1)℃；
(3) different MS culture medium is configured, after the 50d that is commissioned to train, takes aseptic seedling middle and upper part blade, in face of blade cross when inoculation
It cuts to form scratch, vacuum side of blade contacts culture medium；
(4) it is inoculated into addition sucrose, agar solution, adds different growth regulator MS culture mediums, it is dark in phjytotron
It cultivates to be transferred under light after the corresponding time and cultivate, cultivate a couple of days.
Claims (4)
1. a kind of growth regulator for inducing indigo fruit Callus of Leaf, which is characterized in that method includes the following steps:
(1) taking two quoted from Russian table variety Qiu rem and Na rem is test material；
(2) dark culture condition is humidity 75%, temperature (25 ± 1) DEG C；Dark culture condition is humidity 75%, temperature (25 ± 1) DEG C；
(3) different MS culture medium is configured, squamous subculture 30d takes aseptic seedling middle and upper part blade, and when inoculation is crosscutting in face of blade
Scratch is formed, vacuum side of blade contacts culture medium；
(4) it is inoculated into addition sucrose, agar solution, different growth regulator MS culture mediums are added, in phjytotron dark culture
It is transferred under light and cultivates after the corresponding time, cultivate a couple of days.
2. the method for single plant growth regulator induction Callus of Leaf according to claim 1, which is characterized in that
Two kinds described in step (1) pick up from Northeast Agricultural University's gardening experiment centre small berries Germplasm Resources.
3. the method for single plant growth regulator induction Callus of Leaf according to claim 1, which is characterized in that
MS culture medium described in step (3) is respectively 6-BA, 2,5 kinds of hormones of 4-D, TDZ, ZT and CPPU, concentration is 0.5,1.0,
2.0mg·L-1。
4. the method for single plant growth regulator induction Callus of Leaf according to claim 1, which is characterized in that
Sucrose concentration described in step (4) is 30gL-1, agar concentration 7gL-1, it is desirable that it is pH 5.8, cultivates 40 days or so.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811178201.5A CN109156354A (en) | 2018-10-10 | 2018-10-10 | A kind of growth regulator inducing indigo fruit Callus of Leaf |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811178201.5A CN109156354A (en) | 2018-10-10 | 2018-10-10 | A kind of growth regulator inducing indigo fruit Callus of Leaf |
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| CN109156354A true CN109156354A (en) | 2019-01-08 |
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| CN201811178201.5A Pending CN109156354A (en) | 2018-10-10 | 2018-10-10 | A kind of growth regulator inducing indigo fruit Callus of Leaf |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471640A (en) * | 2020-05-06 | 2020-07-31 | 林瑞娥 | Separation and culture method of honeysuckle protoplast and special culture medium |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748462A (en) * | 2005-10-20 | 2006-03-22 | 东北农业大学 | Green branch cuttage breeding method for indigo fruit honeysuckle |
| CN102119657A (en) * | 2010-12-03 | 2011-07-13 | 中国科学院东北地理与农业生态研究所 | Tender stem callus plant regeneration induction culture medium for lonicera caerulea |
| US20130205449A1 (en) * | 2011-08-04 | 2013-08-08 | University Of Connecticut | Methods and Compositions for Production of Triploid Sterile Plants |
| CN104429948A (en) * | 2014-11-17 | 2015-03-25 | 黑龙江省科学院自然与生态研究所 | Lonicera caerulea organ type regeneration culture method |
| CN105409778A (en) * | 2015-12-22 | 2016-03-23 | 广西壮族自治区药用植物园 | Adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca |
-
2018
- 2018-10-10 CN CN201811178201.5A patent/CN109156354A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748462A (en) * | 2005-10-20 | 2006-03-22 | 东北农业大学 | Green branch cuttage breeding method for indigo fruit honeysuckle |
| CN102119657A (en) * | 2010-12-03 | 2011-07-13 | 中国科学院东北地理与农业生态研究所 | Tender stem callus plant regeneration induction culture medium for lonicera caerulea |
| US20130205449A1 (en) * | 2011-08-04 | 2013-08-08 | University Of Connecticut | Methods and Compositions for Production of Triploid Sterile Plants |
| CN104429948A (en) * | 2014-11-17 | 2015-03-25 | 黑龙江省科学院自然与生态研究所 | Lonicera caerulea organ type regeneration culture method |
| CN105409778A (en) * | 2015-12-22 | 2016-03-23 | 广西壮族自治区药用植物园 | Adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca |
Non-Patent Citations (2)
| Title |
|---|
| KARHU, ST: ""Axillary shoot proliferation of blue honeysuckle"", 《PLANT CELL TISSUE AND ORGAN CULTURE》 * |
| 霍俊伟等: ""不同植物生长调节剂对蓝果忍冬叶片愈伤组织诱导的影响"", 《东北农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471640A (en) * | 2020-05-06 | 2020-07-31 | 林瑞娥 | Separation and culture method of honeysuckle protoplast and special culture medium |
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Application publication date: 20190108 |