CN109141963B - Isotope determination sample trace sampling and embedding device - Google Patents
Isotope determination sample trace sampling and embedding device Download PDFInfo
- Publication number
- CN109141963B CN109141963B CN201811133831.0A CN201811133831A CN109141963B CN 109141963 B CN109141963 B CN 109141963B CN 201811133831 A CN201811133831 A CN 201811133831A CN 109141963 B CN109141963 B CN 109141963B
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- Prior art keywords
- internal pressure
- wedge
- embedding
- groove
- spoon
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Links
- 238000005070 sampling Methods 0.000 title claims abstract description 7
- 238000003825 pressing Methods 0.000 claims abstract description 19
- 238000005538 encapsulation Methods 0.000 claims abstract description 12
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 238000013461 design Methods 0.000 claims description 4
- 230000002035 prolonged effect Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 4
- 239000000284 extract Substances 0.000 abstract description 3
- 238000005303 weighing Methods 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000037406 food intake Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000238366 Cephalopoda Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000012443 analytical study Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Abstract
The invention relates to an isotope determination sample trace sampling and embedding device, which comprises a trace sample extraction spoon and an encapsulation embedding device, wherein the trace sample extraction spoon is an integrated structure of a spoon part and an embedding box groove part, and the encapsulation embedding device comprises a pressing plate, a left internal pressure gear, a right internal pressure gear, a high internal pressure wedge and a low internal pressure wedge structure; solves the problem that the experiment cost and the time cost are increased due to the fact that the existing experiment tool repeatedly extracts and weighs samples for many times and the lack of the encapsulation embedding tool.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a trace sample sampling and embedding device for a current isotope mass spectrometer applied to hard tissues such as horny jaws and inner shells of cephalopods, fishes and the like and carbon and nitrogen stable isotopes of soft tissues such as muscles and gonads and the like.
Background
In the research of bioecology such as cephalopods and fishes and reproductive ecology, the measurement and analysis of stable isotopes such as carbon and nitrogen are important research contents. In the process of nutrition relation and breeding energy input distribution of the marine food networks, the feeding sources of the bait organisms, the feeding surplus energy input distribution modes and the like determine the growth and development, and the selection adaptability of the final breeding activities and spawning strategies. With the development of the existing engineering technology and biochemical technology, the stable isotope technology has become a mature place and is widely applied to research of marine organism ingestion ecology and marine organism propagation ecology, and can trace ingestion biological sources of marine organisms, accumulation and distribution of individual ingestion surplus energy and the like. The stable isotope meter has the advantages of simplicity, easy implementation, safety and rapid determination of the equivalent mass of carbon and nitrogen.
Typically, 1.0mg to 1.5mg of trace amounts of powdered sample are extracted and encapsulated for embedding on a dry tissue sample prior to performing a tissue stable isotope assay. However, limited to the current tool limitations of trace sample extraction and encapsulation, it is difficult to extract tissue powdered samples in trace amounts at one time, and repeated extraction-weighing, increased extraction-weighing, even trace extraction-weighing, etc. are required. Thus, repeated steps tend to result in deviations in the final weighed trace sample, which can be quite different for the assay results and subsequent analytical studies; meanwhile, the repeated extraction-weighing steps increase the occurrence of polluted samples to a certain extent, and increase the uncertainty and the error of the measurement result. In addition, after repeated trace extraction-weighing, the current experimental tools cannot conveniently package the embedded samples, increasing the experimental cost and the time cost thereof.
Therefore, in the stable isotope measurement analysis of a tissue sample, it is required to extract a powdery tissue sample in trace amount as simply, rapidly and accurately as possible, and to package and embed the powdery tissue sample rapidly and conveniently, so as to minimize the weighing error of the trace sample, reduce the experimental cost and the time cost thereof, and minimize the occurrence of pollution possibly caused by repeated measurement and extraction processes.
Disclosure of Invention
The technical problem to be solved by the invention is to provide an isotope determination sample trace sampling and embedding device for solving the defects in the background technology.
The technical problems solved by the invention are realized by adopting the following technical scheme:
the trace sample sampling and embedding device for the isotope determination sample comprises a trace sample extraction spoon and an encapsulation embedding device, wherein the trace sample extraction spoon is an integrated structure of a spoon part and an embedding box groove part, and the encapsulation embedding device comprises a pressing plate, a left internal pressure gear, a right internal pressure gear, a high internal pressure wedge and a low internal pressure wedge structure; one surface of the pressing plate is fixed with a left internal pressure gear, a right internal pressure gear, a high internal pressure wedge and a low internal pressure wedge, and is used for packaging the embedding box, and the other surface of the pressing plate is of a full-plane design and is used for compacting the embedding box;
further, the spoon part is a slender rod-shaped extension type, and has a length of about 50.0mm and a diameter of 1.5mm; the tail end of the spoon part is a round shallow groove with the diameter of 1.5mm and the maximum groove depth of 0.3mm;
further, the embedded box groove part is flat and prolonged, and has a length of about 60.0mm, a width of about 11.0mm and a thickness of about 3.0mm; the middle part of the tail end of the embedding box is an open rectangular groove for placing and supporting the embedding box, the length of the groove is about 15.0mm, the width of the groove is about 5.0mm, and the depth of the groove is about 1.8mm;
further, the length of the pressing plate is about 20.0mm, the width of the pressing plate is about 10.5mm, and the thickness of the pressing plate is about 0.6mm;
further, the left and right internal pressure baffles are used for internally pressing the narrow frame of the embedding box, the baffle height is about 3.0mm, the base part is about 3.5mm wide, the thickness is about 0.5mm, the inner side is of a 1/4 circular arc structure, and the radius is 3.0mm;
further, the high and low internal pressure wedges are used for internally pressing a wide frame of the embedding box, the high internal pressure wedges are about 2.5mm in height, the base is about 3.0mm in width, and the wedge bevel angle is about 45 degrees; the low internal pressure wedge is about 1.8mm in height, about 3.0mm in base width, and about 30 DEG wedge bevel;
the invention realizes convenient and accurate extraction, encapsulation and embedding of the trace sample of the stable isotope determination sample, and has the beneficial effects that: the problems that the conventional experimental tool is difficult to simply, conveniently, quickly and accurately extract trace samples, so that weighing deviation is generated in the process of repeatedly extracting and weighing the trace samples for many times, the samples are polluted and the like are solved; solves the problem that the experiment cost and the time cost are increased due to the fact that the existing experiment tool repeatedly extracts and weighs samples for many times and the lack of the encapsulation embedding tool.
Drawings
FIG. 1 is a schematic diagram of a trace sample extraction spoon according to the present invention;
fig. 2 is a schematic diagram of the package embedder according to the present invention.
Detailed Description
The invention is further described with reference to the following detailed drawings in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the implementation of the invention easy to understand.
As shown in fig. 1-2: the trace sample extracting and encapsulating device for stable isotope measurement consists of trace sample extracting spoon, encapsulating embedding device and other two devices.
As shown in fig. 1: the trace sample extraction spoon is an integrated structure of a spoon part 1 and an embedding box groove part 2. The spoon part 1 is a slender rod-shaped extension type and is used for conveniently and accurately extracting trace samples, the length of the spoon part is about 50.0mm, and the diameter of the spoon part is 1.5mm; the tail end is a round shallow groove with the diameter of 1.5mm and the maximum groove depth of 0.3mm. The embedded box groove part 2 is flat and prolonged, has the length of about 60.0mm, the width of about 11.0mm and the thickness of about 3.0mm; the middle part of the tail end is an open rectangular groove for placing and supporting the stable embedding box, the length of the groove is about 15.0mm, the width of the groove is about 5.0mm, and the depth of the groove is about 1.8mm.
As shown in fig. 2: the encapsulation embedding device is composed of a pressing plate 3, a left internal pressure gear 4, a right internal pressure gear 5, a high internal pressure wedge 6, a low internal pressure wedge 7 and the like. The length of the pressing plate 3 is about 20.0mm, the width is about 10.5mm, and the thickness is about 0.6mm; the left internal pressure gear 4, the right internal pressure gear 5, the high internal pressure wedge 6 and the low internal pressure wedge 7 are fixed on one surface of the pressing plate 3 and used for rapidly and conveniently packaging the embedded box, and the other surface is of full-plane design and used for compacting the embedded box. The left and right internal pressure baffles are used for internally pressing the narrow frame of the embedding box, the baffle height is about 3.0mm, the base width is about 3.5mm, the thickness is about 0.5mm, the inner side is of a 1/4 circular arc structure, and the radius is 3.0mm. The high internal pressure wedge and the low internal pressure wedge are used for internally pressing a wide frame of the embedding box, the high internal pressure wedge is about 2.5mm in height, the base part is about 3.0mm in width, and the wedge bevel angle is about 45 degrees; the low internal pressure wedge is about 1.8mm in height, about 3.0mm in base width, and about 30 deg. wedge angle. The design of the high internal pressure wedge and the low internal pressure wedge can enable the wide frames of the embedding box to be pressed in successively, and the situation that the two frames expand outwards due to encapsulation and embedding is avoided.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. The trace sampling and embedding device for the isotope determination sample comprises a trace sample extraction spoon and an encapsulation embedding device, wherein the trace sample extraction spoon is of an integrated structure of a spoon part and an embedding box groove part, the encapsulation embedding device comprises a pressing plate, a left internal pressure gear, a right internal pressure gear, a high internal pressure wedge and a low internal pressure wedge structure, one surface of the pressing plate is fixedly provided with the left internal pressure gear, the right internal pressure gear, the high internal pressure wedge and the low internal pressure wedge, and is used for encapsulating the embedding box, and the other surface of the pressing plate is of a full-plane design and is used for compacting the embedding box;
the spoon part is in a slender rod shape and is about 50.0mm long and 1.5mm in diameter; the tail end of the spoon part is a round shallow groove with the diameter of 1.5mm and the maximum groove depth of 0.3mm;
the embedded box groove part is flat and prolonged, and has a length of about 60.0mm, a width of about 11.0mm and a thickness of about 3.0mm; the middle part of the tail end of the embedding box is an open rectangular groove for placing and supporting the embedding box, the length of the groove is about 15.0mm, the width of the groove is about 5.0mm, and the depth of the groove is about 1.8mm;
the length of the pressing plate is about 20.0mm, the width is about 10.5mm, and the thickness is about 0.6mm;
the left inner pressure rail and the right inner pressure rail are used for inner pressure of a narrow frame of the embedding box, the rail height is about 3.0mm, the base part is about 3.5mm wide, the thickness is about 0.5mm, the inner side is of a 1/4 circular arc structure, and the radius is 3.0mm;
the high internal pressure wedge and the low internal pressure wedge are used for internally pressing a wide frame of the embedding box, the high internal pressure wedge is about 2.5mm in height, the base part is about 3.0mm in width, and the wedge bevel angle is about 45 degrees; the low internal pressure wedge is about 1.8mm in height, about 3.0mm in base width, and about 30 deg. wedge angle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811133831.0A CN109141963B (en) | 2018-09-27 | 2018-09-27 | Isotope determination sample trace sampling and embedding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811133831.0A CN109141963B (en) | 2018-09-27 | 2018-09-27 | Isotope determination sample trace sampling and embedding device |
Publications (2)
| Publication Number | Publication Date |
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| CN109141963A CN109141963A (en) | 2019-01-04 |
| CN109141963B true CN109141963B (en) | 2023-12-19 |
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| CN201811133831.0A Active CN109141963B (en) | 2018-09-27 | 2018-09-27 | Isotope determination sample trace sampling and embedding device |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702773B (en) * | 2019-11-20 | 2020-11-03 | 武汉上谱分析科技有限责任公司 | Method for measuring Pb isotope ratio in sulfide by using MC-ICP-MS |
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