CN109136411A - A kind of method and device controlling bacterial colonization - Google Patents
A kind of method and device controlling bacterial colonization Download PDFInfo
- Publication number
- CN109136411A CN109136411A CN201811047969.9A CN201811047969A CN109136411A CN 109136411 A CN109136411 A CN 109136411A CN 201811047969 A CN201811047969 A CN 201811047969A CN 109136411 A CN109136411 A CN 109136411A
- Authority
- CN
- China
- Prior art keywords
- laser
- chip
- bacterial colonization
- lens
- high speed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The embodiment of the invention discloses a kind of method and devices for controlling bacterial colonization, this method comprises: by bacterial solution to be controlled drop on chip, and through coverslip tabletting, constitute chip sample, the chip sample is placed in above the central through hole for heating warm chamber, together with the chip sample, warm chamber will be heated and be placed in fine motion sample stage center；Laser is opened, and adjusts the position of laser, so that laser facula is focused on the chip sample by adjusting diaphragm and lens, is then shut off laser；The performance number for setting laser is again turned on laser, realizes the control to bacterial colonization.In an embodiment of the present invention, the control to bacterial colonization can be fast implemented in a short time by setting the performance number of laser.
Description
Technical field
The present invention relates to bio-photon chip field more particularly to a kind of method and devices for controlling bacterial colonization.
Background technique
With the fast development of bio-photon chip, bacterium manipulation technology has become the research of bio-photon chip field
Hot spot.Bacterium manipulation technology is widely used in the bacterium selection analysis in the fields such as food hygiene, biomedicine and chemical pharmaceutical
With detection, important technology support is provided for bio-photon chip, to food hygiene, biomedicine, chemical pharmaceutical and environment
The development in the fields such as monitoring even national security is extremely important.
Bacterium control method reported at present is multiple using photoetching and sputtering etc. mostly using glass as chip substrate material
General labourer's skill prepares noble metal electrode on chip substrate surface, recycles the microfluidic channel and metal of dimethyl silicone polymer preparation
Electrode glass chip bonding, finally applies external voltage signal on chip, and the difference of the electrophoretic force being subject to according to bacterium reaches
The manipulation process of pairs of bacterium.
Existing bacterium manipulation technology is using noble metal electrode used in the complicated technologies preparation manipulation bacterium such as photoetching and sputtering
Chip, and introduce external voltage signal.The disadvantages of there are chip structure complexity, installation cost height, non real-time observational record.For
The above problem, the present invention provide that a kind of chip structure is simpler, economy is more excellent, can observe in real time and record bacterium manipulation process
Device and method.
Summary of the invention
The embodiment of the present invention provides a kind of method and device for manipulating bacterial colonization, by setting the performance number of laser,
The control to bacterial colonization can be fast implemented in a short time.
In a first aspect, the embodiment of the invention provides a kind of methods for controlling bacterial colonization, comprising: by bacterium to be controlled
Bacterium solution is dripped on chip, and through coverslip tabletting, constitutes chip sample, and the center that the chip sample is placed in the warm chamber of heating is led to
Above hole, together with the chip sample, the warm chamber of the heating is placed in fine motion sample stage center；
Laser is opened, and adjusts the position of the laser, focuses on laser facula by adjusting diaphragm and lens
The chip sample, is then shut off the laser；
Specific manipulation region is found in the visual field of the high speed camera, is set the performance number of the laser, is opened again
The laser is opened, realizes the control to the bacterial colonization.
Second aspect, the embodiment of the invention provides a kind of device for controlling bacterial colonization, described device includes: light excitation
System, temperature control system and observation system；Wherein,
The smooth activating system manipulates the speed of bacterial colonization by adjusting laser power；
The temperature control system, for controlling the temperature of described device, to provide suitable survival temperature for the bacterium.
The observation system is observed and is recorded in real time the manipulation process of bacterial colonization by camera.
The embodiment of the invention provides a kind of method and devices for controlling bacterial colonization, and bacterial solution to be controlled drop is existed
On chip, and through coverslip tabletting, chip sample is constituted, the chip sample is placed in above the central through hole for heating warm chamber,
Together with the chip sample, the warm chamber of the heating is placed in fine motion sample stage center；Laser is opened, and is adjusted described sharp
The position of light device makes laser facula focus on the chip sample, is then shut off the laser by adjusting diaphragm and lens；
The performance number for setting the laser is again turned on the laser, realizes the control to the bacterial colonization.The present invention is implemented
The method for the control bacterial colonization that example provides is simple, and apparatus structure is simple, cost is relatively low.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, institute in being described below to the embodiment of the present invention
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the invention
Example, for those of ordinary skill in the art, without creative efforts, can also implement according to the present invention
The content of example and these attached drawings obtain other attached drawings.
Fig. 1 is a kind of structural schematic diagram of the device for control bacterial colonization that the embodiment of the present invention five provides；
Fig. 2 is a kind of bacterium agglomeration figure of method for controlling bacterial colonization provided by Embodiment 2 of the present invention；
Fig. 3 is a kind of bacterium agglomeration figure of the method for control bacterial colonization that the embodiment of the present invention three provides；
Fig. 4 is a kind of bacterium agglomeration figure of the method for control bacterial colonization that the embodiment of the present invention four provides；
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention will be further described.
Embodiment one
A kind of process of the method for control bacterial colonization that the embodiment of the present invention one provides.The described method includes:
S110 constitutes chip sample by bacterial solution to be controlled drop on chip, and through coverslip tabletting, will be described
Chip sample is placed in above the central through hole for heating warm chamber, together with the chip sample, the warm chamber of the heating is placed in micro-
Dynamic sample stage center；
S120 opens laser, and adjusts the position of the laser, keeps laser facula poly- by adjusting diaphragm and lens
Coke is then shut off the laser in the chip sample；
S130 sets the performance number of the laser, is again turned on the laser, realizes the control to the bacterial colonization
System.
In step s 110, it needs that chip and coverslip are first carried out sterilization treatment before operation.
In the step s 120, the adjusting laser facula focuses on chip sample, should choose chip sample side
The aseptic area of edge.
In step s 130, it is described be again turned on laser before, fine motion sample stage need to be manually adjusted and be positioned at chip sample
Specific manipulation region.
Optionally, in the opening laser, and the position of the laser is adjusted, makes to swash by adjusting diaphragm and lens
Light hot spot focuses on before the chip sample, the method also includes: high speed camera and backlight are opened, described in adjusting
Fine motion sample stage makes chip sample edge be located at the near focal point of object lens, finds spy in the visual field of the high speed camera
Surely region is manipulated, adjusts the object lens until observing clearly bacterium image in the visual field of the high speed camera.
Optionally, while the control realized to the bacterial colonization, further includes: open the high speed camera
RECORD mode carries out observation in real time and record using process of the high speed camera to the bacterial colonization.
Optionally, the chip in the chip sample is lithium niobate crystal chip.
Optionally, the warm chamber of the heating is for providing suitable survival temperature for the bacterium.
In embodiment, bacterial solution to be controlled is dripped on lithium niobate crystal chip surface and through coverslip tabletting, by tabletting
Lithium niobate crystal chip afterwards is placed in above the central through hole of temperature control system temperature chamber, and warm chamber is placed in transparent glass together with lithium niobate crystal chip
Fine motion sample stage center；High speed camera is opened, backlight is opened, transparent glass fine motion sample stage is adjusted, makes lithium niobate crystal chip side
Edge is located near object focal point, continues to finely tune, and clearly bacterium image is observed in the high speed camera visual field；Laser is opened,
Laser positions are adjusted, is adjusted after laser facula focuses on lithium niobate crystal chip by diaphragm and lens and closes laser；In high speed
Specific manipulation region required for finding in camera fields of view sets laser power levels, opens high speed camera RECORD mode, again
Laser is opened, observes the overall process with record manipulation bacterial colonization in real time using high speed camera.
Wherein, laser irradiation can inspire the body photovoltaic effect of lithium niobate, in lithium niobate crystal chip in lithium niobate crystal chip
Inside generates the directional migration that carrier is swashed along the light of Z-direction, for lithium niobate C piece, so that lithium niobate+C is in face of outer
Show elecrtonegativity ,-C faces outer display electropositive, and a non-uniform electric field is formed inside lithium niobate crystal chip；For lithium niobate
For Y piece, crystals light swashs carrier equally along Z-direction directional migration, so that lithium niobate Y piece surface formation one is non-even
Strong electrical field.To generate dielectrophoretic force to bacterium, so that bacterium gathers.By adjusting the height of laser power, can adjust
The size of dielectrophoretic force is controlled, and then flexibly controlling bacterium gathers speed.
The laser 1, it is desirable that laser irradiation excites generation body photovoltaic effect to avoid purple simultaneously in lithium niobate crystal chip
Influence of the outer light to bacterial activity, therefore its wave-length coverage is selected in 400~650nm, 0.5~50mW of power working range；
The focal length value range of first lens is 10~150mm, and the focal length value range of the second lens is 50~350mm；Temperature control instrument control
Warm range is 20~100 DEG C, and the face+C, the face-C and the face Y can be selected in lithium niobate crystal chip；20~100 times of object lens enlargement ratio.
In summary the use scope of each device and consider the cost and resultant effect of element, the preferred scope of each parameter
Be: laser wavelength range is 405~580nm, and laser power range is 5~25mW；The focal length value range of first lens is
20~100mm, the focal length value range of the second lens are 100~300mm；The control of warm chamber temperature is at 36~38 DEG C, lithium niobate crystal
Piece selects the face Y；50~100 times of object lens enlargement ratio, bacterium selects escherichia coli (ATCC 25922).
Embodiment two
Fig. 2 is a kind of bacterium agglomeration figure of method for controlling bacterial colonization provided by Embodiment 2 of the present invention.It uses
405nm laser, backlight select halogen bulb, and the second optical filter selects 533nm green color filter, and the focal length of the first lens is
25mm, the focal length of the second lens are 200mm；100 times of object lens magnification, 37 DEG C of warm chamber temperature, the niobic acid after sterilization
25922 bacterium solution of ATCC is added dropwise in the lithium face chip Y, and uses the tabletting of sterile glass coverslip, is placed at warm chamber central through hole, opens
High speed camera opens backlight and adjusts transparent glass micromotion platform, clearly bacterium image is collected on high speed camera, opens
High speed camera RECORD mode opens laser, and adjusting laser power is 12.3mW, and the insertion of the position shown in device figure first filters
Piece (405nm negativity optical filter) can observe bacterium used time 30s at high speed camera display screen and complete collection at laser facula
It is poly-.
Embodiment three
Fig. 3 is a kind of bacterium agglomeration figure of the method for control bacterial colonization that the embodiment of the present invention three provides.It uses
473nm laser, backlight select halogen bulb, and the second optical filter selects 533nm green color filter, and the focal length of the first lens is
25mm, the focal length of the second lens are 200mm；100 times of object lens magnification, 37 DEG C of warm chamber temperature, the niobic acid after sterilization
25922 bacterium solution of ATCC is added dropwise in the lithium face chip Y, and uses the tabletting of sterile glass coverslip, is placed at warm chamber central through hole, opens
High speed camera opens backlight and adjusts transparent glass micromotion platform, clearly bacterium image is collected on high speed camera, opens
High speed camera RECORD mode opens laser, and adjusting laser power is 8.8mW, and the insertion of the position shown in device figure first filters
Piece (473nm negativity optical filter) can observe bacterium used time 90s at high speed camera display screen and complete collection at laser facula
It is poly-.
Example IV
Fig. 4 is a kind of bacterium agglomeration figure of the method for control bacterial colonization that the embodiment of the present invention four provides.It uses
532nm laser, backlight select halogen bulb, and the focal length of the first lens is 25mm, and the focal length of the second lens is 200mm；Object
100 times of mirror amplification factor, 25922 bacterium solution of ATCC is added dropwise in 37 DEG C of warm chamber temperature, the face lithium niobate crystal chip Y after sterilization, and
It using sterile glass coverslip tabletting, is placed at warm chamber central through hole, opens high speed camera, open backlight and adjust transparent glass
Micromotion platform collects clearly bacterium image on high speed camera, opens high speed camera RECORD mode, opens laser, adjusts
Section laser power is 10.6mW, and the first optical filter (532nm negativity optical filter) is inserted into the position shown in device figure, and high speed camera is aobvious
It can observe bacterium used time 150s at display screen and complete gathering at laser facula.
Embodiment five
Fig. 1 is a kind of structural schematic diagram of the device for control bacterial colonization that the embodiment of the present invention five provides.Such as Fig. 1 institute
Show, which includes: light activating system and temperature control system；Wherein, the smooth activating system, by adjusting laser power pair
The speed of bacterial colonization is manipulated；The temperature control system, for controlling the temperature of described device, to be provided for the bacterium
Suitable survival temperature.
Optionally, described device further include: the observation system, for observing the manipulation process of the bacterial colonization.
Optionally, light activating system includes: laser, diaphragm, the first lens；
Temperature control system includes: to heat warm chamber and temperature controller；
Observation system includes: the second lens, optical filter, chip sample, fine motion sample stage, object lens, camera and backlight.
Optionally, the temperature control system utilizes resistance heating, and utilizes platinum probe thermometric, the bottom tool for heating warm chamber
There is central through hole.
Optionally, it is right when the smooth activating system, the temperature control system and the observation system coordinate synchronization operate
The manipulation process of bacterial colonization is observed and is recorded in real time.
In embodiment, described device includes: laser 1, diaphragm 2, the first lens 3, the optical filtering of semi-transparent semi-reflecting lens 4, first
Piece 5, the second lens 7, object lens 8, chip sample 9, heats warm chamber 10, temperature controller 11, fine motion sample stage 12, second at high speed camera 6
Optical filter 13, backlight 14.
Wherein, laser 1, diaphragm 2, the first lens 3, the light activating system of constituent apparatus.Heat warm chamber 10, temperature controller
11, the temperature control system of constituent apparatus.Semi-transparent semi-reflecting lens 4, the first optical filter 5, high speed camera 6, the second lens 7, object lens 8, chip
Sample 9, fine motion sample stage 12, the second optical filter 13, backlight 14, the observation system of constituent apparatus.
Note that the above is only a better embodiment of the present invention and the applied technical principle.It will be appreciated by those skilled in the art that
The invention is not limited to the specific embodiments described herein, be able to carry out for a person skilled in the art it is various it is apparent variation,
It readjusts and substitutes without departing from protection scope of the present invention.Therefore, although being carried out by above embodiments to the present invention
It is described in further detail, but the present invention is not limited to the above embodiments only, without departing from the inventive concept, also
It may include more other equivalent embodiments, and the scope of the invention is determined by the scope of the appended claims.
Claims (10)
1. a kind of method for controlling bacterial colonization, which is characterized in that the described method includes:
By bacterial solution to be controlled drop on chip, and through coverslip tabletting, chip sample is constituted, the chip sample is set
Above the central through hole for heating warm chamber, together with the chip sample, the warm chamber of the heating is placed in fine motion sample stage
Centre；
Laser is opened, and adjusts the position of the laser, focuses on laser facula by adjusting diaphragm and lens described
Chip sample is then shut off the laser；
The performance number for setting the laser is again turned on the laser, realizes the control to the bacterial colonization.
2. the method according to claim 1, wherein in the opening laser, and adjusting the laser
Position, before so that laser facula is focused on the chip sample by adjusting diaphragm and lens, the method also includes:
High speed camera and backlight are opened, the fine motion sample stage is adjusted, chip sample edge is made to be located at the focus of object lens
Near, specific manipulation region is found in the visual field of the high speed camera, adjusts the object lens until in the high speed camera
Clearly bacterium image is observed in the visual field.
3. according to the method described in claim 2, it is characterized in that, realizing the same of the control to the bacterial colonization described
When, further includes:
The RECORD mode for opening the high speed camera is seen in real time using process of the high speed camera to the bacterial colonization
It surveys and records.
4. the method according to claim 1, wherein the chip in the chip sample is lithium niobate crystal chip.
5. the method according to claim 1, wherein heating temperature chamber is suitable for providing for the bacterium
Survival temperature.
6. a kind of device for manipulating bacterial colonization, which is characterized in that described device includes: light activating system and temperature control system；
Wherein,
The smooth activating system manipulates the speed of bacterial colonization by adjusting laser power；
The temperature control system, for controlling the temperature of described device, to provide suitable survival temperature for the bacterium.
7. device according to claim 6, which is characterized in that described device further include:
Observation system, for observing the manipulation process of the bacterial colonization.
8. device according to claim 7, which is characterized in that
The smooth activating system includes: laser, diaphragm, the first lens；
The temperature control system includes: to heat warm chamber and temperature controller；
The observation system includes: the second lens, optical filter, chip sample, fine motion sample stage, object lens, camera and backlight.
9. device according to claim 5, which is characterized in that the temperature control system utilizes resistance heating, and is visited using platinum
Head thermometric, the bottom for heating warm chamber have central through hole.
10. device according to claim 5, which is characterized in that the smooth activating system, the temperature control system, Yi Jisuo
When stating the operating of observation system coordinate synchronization, the manipulation process of bacterial colonization is observed and recorded in real time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811047969.9A CN109136411B (en) | 2018-09-10 | 2018-09-10 | Method and device for controlling bacteria colony |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811047969.9A CN109136411B (en) | 2018-09-10 | 2018-09-10 | Method and device for controlling bacteria colony |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109136411A true CN109136411A (en) | 2019-01-04 |
| CN109136411B CN109136411B (en) | 2022-07-22 |
Family
ID=64824170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811047969.9A Active CN109136411B (en) | 2018-09-10 | 2018-09-10 | Method and device for controlling bacteria colony |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109136411B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040067482A1 (en) * | 2000-11-22 | 2004-04-08 | Kenji Yasuda | Apparatus for microscopic observation of long-term culture of single cell |
| US20060141624A1 (en) * | 2002-05-23 | 2006-06-29 | Karsten Koenig | Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method |
| US20090052021A1 (en) * | 2005-09-26 | 2009-02-26 | Hideo Mogami | Microscopic Cell Observation and Inspection System Using a Plurality of Observation Methods |
| US20150185456A1 (en) * | 2013-12-27 | 2015-07-02 | Sony Corporation | Microscope system and control method therefor |
-
2018
- 2018-09-10 CN CN201811047969.9A patent/CN109136411B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040067482A1 (en) * | 2000-11-22 | 2004-04-08 | Kenji Yasuda | Apparatus for microscopic observation of long-term culture of single cell |
| US20060141624A1 (en) * | 2002-05-23 | 2006-06-29 | Karsten Koenig | Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method |
| US20090052021A1 (en) * | 2005-09-26 | 2009-02-26 | Hideo Mogami | Microscopic Cell Observation and Inspection System Using a Plurality of Observation Methods |
| US20150185456A1 (en) * | 2013-12-27 | 2015-07-02 | Sony Corporation | Microscope system and control method therefor |
Non-Patent Citations (1)
| Title |
|---|
| 王海灵: "《学生科普百科知识三十讲能量大集合-激光》", 30 April 2009, 北京燕山出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109136411B (en) | 2022-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | Leukemic cell intracellular responses to nanosecond electric fields | |
| CN106772990B (en) | A kind of light control techniques for realizing the adjustment of cell tandem using double optical fiber optical tweezers | |
| Gong et al. | Upconversion nanoparticle decorated spider silks as single-cell thermometers | |
| JP2008076411A (en) | Convex geometry adhesive film system for laser capture microdissection | |
| Scarisoreanu et al. | Lead-free piezoelectric (Ba, Ca)(Zr, Ti) O3 thin films for biocompatible and flexible devices | |
| JP4967554B2 (en) | Cell capture device and temperature control method for cell capture device | |
| JP2013094168A (en) | Method and apparatus for myocardial toxicity test and cardiomyocyte evaluation | |
| CN107843541A (en) | A kind of real-time monitoring system and method for unicellular organism physical characteristic | |
| CN111617390B (en) | Device for regulating and controlling red blood cells in living animal blood vessel | |
| CN108872084A (en) | A kind of full light microlayer model separation method based on asymmetric lithium niobate sandwich | |
| CN109136411A (en) | A kind of method and device controlling bacterial colonization | |
| Suzuki et al. | Individual-cell-based electrophysiological measurement of a topographically controlled neuronal network pattern using agarose architecture with a multi-electrode array | |
| WO2023019431A1 (en) | Device and method for stimulating secretion of cellular exosomes, and obtained exosomes and application thereof | |
| CN102586092B (en) | Cell impedance imaging system and cell impedance imaging method for dynamically monitoring wound healing | |
| CN111763622A (en) | Living cell in-vitro in-situ culture testing system for magnetic drive micro-nano robot | |
| CN106017738A (en) | High-temperature dynamic testing system and method for thin film thermocouple | |
| JP3166144B2 (en) | Micro laboratory system | |
| CN106367344B (en) | A kind of cell minute yardstick heating culture control device | |
| US20210380919A1 (en) | Laser assisted metal adhesion to indium tin oxide on glass, quartz, sapphire and single crystal silicon wafer substrates for heated platforms for cell culturing | |
| US11578298B2 (en) | Device for the cultivation of and radiation-induced killing of cells and method for analyzing a migration and/or healing of a wound | |
| CN208762509U (en) | Nucleic acid detection apparatus and system | |
| CN108795695A (en) | A kind of method and device for optical tweezer cell and particle to cold and hot formula device | |
| JP2009229308A (en) | Method and apparatus for cell activity measurement | |
| Liao et al. | Graphene photothermal effect-induced microbubble for microparticle manipulation | |
| RU2129266C1 (en) | Device automatically recording dynamic characteristics of progress of process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |