CN108795695A - A kind of method and device for optical tweezer cell and particle to cold and hot formula device - Google Patents
A kind of method and device for optical tweezer cell and particle to cold and hot formula device Download PDFInfo
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- CN108795695A CN108795695A CN201810741219.5A CN201810741219A CN108795695A CN 108795695 A CN108795695 A CN 108795695A CN 201810741219 A CN201810741219 A CN 201810741219A CN 108795695 A CN108795695 A CN 108795695A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The present invention provides a kind of method and device for optical tweezer cell and particle to cold and hot formula device,The method includes the selections of cell,The label of cell tissue,The optical tweezer of cell acts on,The cartonning operation of cell and observing and recording for cell,Described device includes cold-hot type experimental box and spherical laser wave machine,The spherical laser wave machine is welded on cold-hot type experimental box,Control panel has been bolted at the top of the experimental box,The side of the experimental box is equipped with electric sealed door,Concentrating refractive device has been bolted in the bottom of the spherical laser wave machine,Optical tweezer, which has been bolted, in the bottom of the concentrating refractive device wears and penetrates head,Electronic fixator has been bolted on the cold-hot type experimental box,The bottom of the cold-hot type experimental box is welded with self-adjustable fixed seat,The side of the cold-hot type experimental box is welded with observation panel,Microcosmic probe has been bolted in the inside of the cold-hot type experimental box.
Description
Technical field
The present invention be it is a kind of for optical tweezer cell and particle to the method and device of cold and hot formula device, belong to optical tweezer research skill
Art field.
Background technology
Optical tweezer is when acting on particle or cell surface using spherical laser wave and single beam gradient force ligh trap, and generates power
Effect a kind of optical technology, gradient force ligh trap can limit the spatial position of the particle and cell that are applied, to realize
Cell is acted on every empty tweezer, nowadays some researches show that when having a kind of zooblast to carry out one section of culture at different temperatures
Between, cell membrane and cytoplasm tool change significantly, and by its tweezer comes out from experimental box when, common cell tweezer be easy
Destroy the cell membrane of cell, it is therefore desirable to it is a kind of can be every sky by the method and device of cell tweezer.
For this purpose, the present invention proposes a kind of method and device for optical tweezer cell and particle to cold and hot formula device.
Invention content
In view of the deficienciess of the prior art, it is an object of the present invention to provide one kind for optical tweezer cell and particle to cold-hot type
The method and device of device, to solve the problems mentioned in the above background technology, the present invention are novel in design, reasonable for structure, can
High accurancy and precision by studied cell every under empty tweezer to electron microscope, avoid damage to its cell membrane.
To achieve the goals above, the present invention is to realize by the following technical solutions：One kind for optical tweezer cell with
Particle to cold and hot formula device method, including it is following：
The selection of cell tissue：Experimenter selects a physiological status and preferably grows up experiment live body first, then logical
It crosses sterile knife and cuts a fritter organ-tissue, then trained in organ-tissue tweezer to Tissue Culture Dish under sterile environment
It supports and needs to be placed with suitable inorganic salts, carbohydrate, amino acid, vitamin and the necessary trace element of cellular physiological events in ware；
The label of cell tissue：Experimenter adds in the culture dish for being contained with experiment organ-tissue carries radioactivity
The medicament of nucleic, and the surface of organ-tissue is fully immersed among medicament, radionuclide is also known as isotope, and this
The isotope P 32 that experiment uses phosphorus be active nucleus mixing in a medicament, when organ-tissue is immersed in by P 32 and organic molten
In the medicament of agent composition, the one layer of cells on organ-tissue surface can be stained with isotope P 32, therefore can be by observation
No carrying P 32 distinguishes cell；
The optical tweezer of cell acts on：Organ-tissue from culture dish, is worn using sterile folder tweezer to optical tweezer and is penetrated by experimenter
It in the culture dish of head bottom, is then switched by the fixator controlled on control panel, subsequent electronic fixator will will operate
Ware is fixed on the positioning so that then the case where operation ware is not in mobile during operation and inclination controls successively
Optical pressure switchs and refraction knob is then revolved by reflecting to make optical pressure generator emit the light beam with energy vertically downward
Button adjusts the angle of the electronic enocscope in concentrating refractive device, the further angle for adjusting light beam, to energy
Light beam is worn by optical tweezer penetrates the surface that head is applied to cell, since the light beam zone of action cell surface with energy will produce gradient
Power ligh trap, gradient force ligh trap can limit the spatial position of cell, to achieve the purpose that cell every empty tweezer, pass through
The method, every 4 cells and 1 cell without P 32 for carrying P 32 of empty tweezer on organ-tissue from operation ware；
The cartonning of cell operates：By controlling, switch or knob make optical tweezer is worn to penetrate to experimenter accordingly on control panel
Head no longer emits light beam, therefore cell is no longer acted on by the light beam with energy, can then drop into experiment ware, by this side
Formula carries out 5 operations so that and 4 there is P 32 and 1 normal experimental cell to respectively fall in into 5 identical experiment wares,
It tests in ware with carbohydrate, vitamin, amino acid and the trace element needed for cellular physiological events, then manipulates on control panel
Sealing door switch so that electric sealed door is opened, and then by 4, the experimental cells with P 32 are placed individually into cold-hot type reality
4 different temperature cabins in tryoff, temperature is respectively 10,20, -10 and -20 DEG C, and another is individually tested containing normal
The experiment ware of cell is placed into the environment of room temperature germ-free condition, is then closed electric sealed door；
Cell observes and records：After cell is placed into cold-hot type experimental box by experimenter, after waiting for a couple of days, respectively will
4 the experiment wares of experimental cell and the experiment ware of 1 normal experimental cell with P 32 are placed under electron microscope and carry out
Observation, experimenter need whether physiological status, cell membrane and the cytoplasm of observing 5 cells have significant change, and will experiment
The data of process and result are recorded.
A kind of device for optical tweezer cell and particle to cold and hot formula device, including cold-hot type experimental box and spherical laser wave
Machine, the spherical laser wave machine are welded on cold-hot type experimental box, behaviour have been bolted at the top of the cold-hot type experimental box
Panel is controlled, the side of the cold-hot type experimental box is equipped with electric sealed door, and the bottom of the spherical laser wave machine passes through bolt
It is connected with concentrating refractive device, optical tweezer, which has been bolted, in the bottom of the concentrating refractive device wears and penetrate head, the cold-hot type experiment
Electronic fixator is bolted on case, the bottom of the cold-hot type experimental box is welded with self-adjustable fixed seat, described cold
The side of hot type experimental box is welded with observation panel, and microcosmic probe has been bolted in the inside of the cold-hot type experimental box.
Optical pressure has been bolted respectively as a kind of preferred embodiment of the present invention, on the control panel to open
It closes, refraction knob, sealing door switch and fixator switch, the interior welds of the control panel have data mainboard, the data
Mainboard is connected with fixator switch, sealing door switch, refraction knob and optical pressure switch respectively by electric wire.
As a kind of preferred embodiment of the present invention, the interior welds of the spherical laser wave machine have optical pressure generator,
The quantity of the optical pressure generator is a, and the optical pressure switch is connect by electric wire with optical pressure generator.
As a kind of preferred embodiment of the present invention, electric folding has been bolted in the inside of the concentrating refractive device
The quantity of light microscopic, the electronic enocscope is 2, and the refraction knob is connect by electric wire with electronic enocscope.
As a kind of preferred embodiment of the present invention, the cold-hot type experimental box is internally provided with temperature cabin, described
The quantity in temperature cabin is 4, and the set temperature in the temperature cabin is respectively 10,20, -10 and -20 DEG C, and the microcosmic probe passes through
Data line is connect with the data mainboard in control panel.
As a kind of preferred embodiment of the present invention, lifting has been bolted in the inside of the self-adjustable fixed seat
Bar, horizon sensor is welded at the top of the cold-hot type experimental box, and the horizon sensor passes through level monitoring circuit and rises
Bar is dropped to be electrically connected.
As a kind of preferred embodiment of the present invention, dual-layer vacuum glass is welded on the observation panel.
As a kind of preferred embodiment of the present invention, the quantity of the microcosmic probe and self-adjustable fixed seat is 4.
As a kind of preferred embodiment of the present invention, the bottom of the cold-hot type experimental box is welded with alarming instrument, described
Alarming instrument is electrically connected by electric wire and the data mainboard in manipulation plate face.
Beneficial effects of the present invention：
1. this is used for optical tweezer cell and the method and device of particle to cold and hot formula device generates band by spherical laser wave machine
There is the light beam of energy and momentum to act on the surface of cell, to form ligh trap, realizes and tweezer is carried out to cell every sky
The problem of effect, such mode avoids damaging cells film in experimental implementation, therefore easily facilitate experimenter and test
Research, improves the successful of experiment；
2. this is used for optical tweezer cell and uses isotope-labelling method to the method and device of cold and hot formula device with particle, will be thin
Born of the same parents are marked, and can effectively distinguish experimental cell and ordinary cells, convenient for being compared reference when observing simultaneously
Effect；
3. this is used for the firm banking that optical tweezer cell is provided with self-adjustable with the method and device of particle to cold and hot formula device,
The horizontality of babinet can be detected according to the horizon sensor of top of the box, thus the level of automatic regulating device entirety,
Improve the order of accuarcy of experiment.
4. this is used for optical tweezer cell and the method and device of particle to cold and hot formula device is novel in design, reasonable for structure, can
High accurancy and precision by studied cell every under empty tweezer to electron microscope, avoid damage to its cell membrane.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram for optical tweezer cell and device of the particle to cold and hot formula device of the invention；
Fig. 2 is a kind of flow chart for optical tweezer cell and method of the particle to cold and hot formula device of the invention；
In figure：1- cold-hot types experimental box, 2- control panels, 3- electric sealeds door, 4- spherical laser waves machine, 5- concentrating refractives
Device, 6- optical tweezers, which are worn, penetrates the microcosmic probe of the electronic fixator of head, 7-, 8-, 9- observation panels, 10- self-adjustables fixed seat, 11- horizontal sensors
Device.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, with reference to
Specific implementation mode, the present invention is further explained.
It please refers to Fig.1 to Fig. 2, the present invention provides a kind of technical solution：One kind is for optical tweezer cell and particle to cold-hot type
The method of device, including it is following：
The selection of cell tissue：Experimenter selects a physiological status and preferably grows up experiment live body first, then logical
It crosses sterile knife and cuts a fritter organ-tissue, then trained in organ-tissue tweezer to Tissue Culture Dish under sterile environment
It supports and needs to be placed with suitable inorganic salts, carbohydrate, amino acid, vitamin and the necessary trace element of cellular physiological events in ware；
The label of cell tissue：Experimenter adds in the culture dish for being contained with experiment organ-tissue carries radioactivity
The medicament of nucleic, and the surface of organ-tissue is fully immersed among medicament, radionuclide is also known as isotope, and this
The isotope P 32 that experiment uses phosphorus be active nucleus mixing in a medicament, when organ-tissue is immersed in by P 32 and organic molten
In the medicament of agent composition, the one layer of cells on organ-tissue surface can be stained with isotope P 32, therefore can be by observation
No carrying P 32 distinguishes cell；
The optical tweezer of cell acts on：Organ-tissue from culture dish, is worn using sterile folder tweezer to optical tweezer and is penetrated by experimenter
It in the culture dish of first 6 bottom, is then switched by the fixator controlled on control panel 2, subsequent electronic fixator 7 will will be grasped
It is fixed on the positioning as ware so that then the case where operation ware is not in mobile during operation and inclination is controlled successively
Optical pressure switch and refraction knob processed then pass through refraction to make optical pressure generator emit the light beam with energy vertically downward
Knob adjusts the angle of the electronic enocscope in concentrating refractive device 5, the further angle for adjusting light beam, to carry energy
Light beam worn by optical tweezer and penetrate first 6 surfaces for being applied to cell, since the light beam zone of action cell surface with energy will produce
Gradient force ligh trap, gradient force ligh trap can limit the spatial position of cell, to achieve the purpose that carry out cell every empty tweezer,
By the method, from operation ware on organ-tissue every empty tweezer 4 with P 32 cells and 1 without the thin of P 32
Born of the same parents；
The cartonning of cell operates：By controlling, switch or knob make optical tweezer wear to experimenter accordingly on control panel 2
It penetrates first 6 and no longer emits light beam, therefore cell is no longer acted on by the light beam with energy, can then drop into experiment ware, press
This mode carries out 5 operations so that 4 there is P 32 and 1 normal experimental cell to respectively fall in 5 identical experiment wares
In, with carbohydrate, vitamin, amino acid and the trace element needed for cellular physiological events in experiment ware, then manipulate operation control surface
Sealing door switch on plate 2 so that electric sealed door 3 is opened, and is then placed individually into 4 experimental cells with P 32 cold
4 different temperature cabins in hot type experimental box 1, temperature is respectively 10,20-10 and -20 DEG C, and another individually contains just
The experiment ware of normal experimental cell is placed into the environment of room temperature germ-free condition, is then closed electric sealed door 3；
Cell observes and records：After cell is placed into cold-hot type experimental box 1 by experimenter, after waiting for a couple of days, respectively
By 4 with P 32 the experiment wares of experimental cell and the experiment ware of 1 normal experimental cell be placed under electron microscope into
Row observation, experimenter need whether physiological status, cell membrane and the cytoplasm of observing 5 cells have significant change, and will be real
The data for testing process and result are recorded.
A kind of device for optical tweezer cell and particle to cold and hot formula device, including cold-hot type experimental box 1 and light radiation pressure
Power machine 4, the spherical laser wave machine 4 are welded on cold-hot type experimental box 1, and the top of the cold-hot type experimental box 1 is connected by bolt
It is connected to control panel 2, the side of the cold-hot type experimental box 1 is equipped with electric sealed door 3, the bottom of the spherical laser wave machine 4
Concentrating refractive device 5 has been bolted in portion, and optical tweezer, which has been bolted, in the bottom of the concentrating refractive device 5 wears and penetrate first 6, institute
It states and electronic fixator 7 has been bolted on cold-hot type experimental box 1, the bottom of the cold-hot type experimental box 1 is welded with self-regulated
The side of formula fixed seat 10, the cold-hot type experimental box 1 is welded with observation panel 9, and the inside of the cold-hot type experimental box 1 passes through spiral shell
It tethers and is connected to microcosmic probe 8.
Optical pressure has been bolted respectively as a kind of preferred embodiment of the present invention, on the control panel 2 to open
It closes, refraction knob, sealing door switch and fixator switch, the interior welds of the control panel 2 have data mainboard, the data
Mainboard is connected with fixator switch, sealing door switch, refraction knob and optical pressure switch respectively by electric wire.
As a kind of preferred embodiment of the present invention, the interior welds of the spherical laser wave machine 4 have optical pressure generator,
The quantity of the optical pressure generator is 2, and the optical pressure switch is connect by electric wire with optical pressure generator.
As a kind of preferred embodiment of the present invention, the inside of the concentrating refractive device 5 has been bolted electronic
The quantity of enocscope, the electronic enocscope is 2, and the refraction knob is connect by electric wire with electronic enocscope.
As a kind of preferred embodiment of the present invention, the cold-hot type experimental box 1 is internally provided with temperature cabin, described
The quantity in temperature cabin is 4, and the set temperature in the temperature cabin is respectively 10,20, -10 and -20 DEG C, and the microcosmic probe 8 is logical
Data line is crossed to connect with the data mainboard in control panel 2.
As a kind of preferred embodiment of the present invention, liter has been bolted in the inside of the self-adjustable fixed seat 10
Bar drops, and the top of the cold-hot type experimental box 1 is welded with horizon sensor 11, and the horizon sensor 11 passes through level monitoring electricity
Road is electrically connected with elevating lever.
As a kind of preferred embodiment of the present invention, dual-layer vacuum glass is welded on the observation panel 9.
As a kind of preferred embodiment of the present invention, the quantity of the microcosmic probe 8 and self-adjustable fixed seat 10 is 4
It is a.
As a kind of preferred embodiment of the present invention, the bottom of the cold-hot type experimental box 1 is welded with alarming instrument, described
Alarming instrument is electrically connected by electric wire and the data mainboard in manipulation plate face 2.
Operation principle：This includes the selection of cell, groups of cells for optical tweezer cell and the method for particle to cold and hot formula device
Optical tweezer effect, the cartonning operation of cell and the observing and recording for cell of the label, cell knitted, used P 32 wherein in method
It after adhesion is on cell, observes under the microscope, the cell with P 32 element has the effect of colour developing, for seeing at the same time
When examining the cell under experimental cell and room temperature, the effect that the two is distinguished, this is used for optical tweezer cell and is filled with particle to cold-hot type
The device set includes cold-hot type experimental box 1 and spherical laser wave machine 4, and 2 optical pressure that are internally provided with of spherical laser wave machine 4 occur
Device goes out the light beam that twice carry energy momentum for Vertical Launch, and the concentrating refractive device 5 of 4 bottom of spherical laser wave machine
2 electronic enocscopes are internally provided with, according to experimenter by manipulating refractive power knob, to adjust the angle of electronic enocscope,
To have adjusted the angle of twice light beam being refracted, subsequent light beam is worn by optical tweezer to be penetrated first 6 and is applied on cell surface, is carried
The light beam of energy and momentum, which acts on, can generate gradient force ligh trap on cell surface, gradient force ligh trap can limit the sky of cell
Between position, therefore can according to the angle for adjusting light beam realize to cell every empty tweezer effect, and by adjusting light radiation
The output power of forcing press 4 controls the strength of tweezer, the effect of this respective outer side edges just can realize every empty tweezer cell or
The effect of particle, and the self-adjustable fixed seat 10 of bottom of device can be according to the detection cold-hot type of horizon sensor 11 experimental box 1
No to be in horizontality, if cold-hot type experimental box 1 is not at horizontality, the elevating lever in self-adjustable fixed seat 10 starts automatically
Flexible, until cold-hot type experimental box 1 is in horizontality, this structure is used to ensure the level of cold-hot type experimental box 1, reduces experiment
Error, this is used for optical tweezer cell and particle to the method and device novel design of cold and hot formula device, reasonable for structure, can be high-precision
Accuracy by studied cell every under empty tweezer to electron microscope, avoid damage to its cell membrane novel design.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill
For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from the present invention spirit or
In the case of essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action
Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state
Bright restriction, it is intended that including all changes that come within the meaning and range of equivalency of the claims in the present invention
It is interior.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (10)
1. a kind of method for optical tweezer cell and particle to cold and hot formula device, which is characterized in that including following：
The selection of cell tissue：Experimenter select first a physiological status preferably grow up experiment live body, then pass through nothing
Bacterium knife cuts a fritter organ-tissue, then under sterile environment by organ-tissue tweezer to Tissue Culture Dish, culture dish
In need to be placed with suitable inorganic salts, carbohydrate, amino acid, vitamin and the necessary trace element of cellular physiological events；
The label of cell tissue：Experimenter adds in the culture dish for being contained with experiment organ-tissue carries radionuclide
Medicament, and the surface of organ-tissue is fully immersed among medicament, radionuclide is also known as isotope, and this is tested
The isotope P 32 for using phosphorus is that active nucleus mixes in a medicament, when organ-tissue is immersed in by P 32 and organic solvent group
At medicament in, the one layer of cells on organ-tissue surface can be stained with isotope P 32, therefore can be by seeing whether to take
Band P 32 distinguishes cell；
The optical tweezer of cell acts on：Organ-tissue from culture dish, is worn using sterile folder tweezer to optical tweezer and penetrates head (6) by experimenter
It in the culture dish of bottom, is then switched by the fixator controlled on control panel (2), subsequent electronic fixator (7) will incite somebody to action
Operation ware is fixed on the positioning so that the case where operation ware is not in mobile during operation and inclination, then successively
Optical pressure switch and refraction knob are controlled, to make optical pressure generator emit the light beam with energy vertically downward, then passes through folding
Knob is penetrated to adjust the angle of the electronic enocscope in concentrating refractive device (5), the angle of light beam is further adjusted, to carry
The light beam of energy is worn by optical tweezer penetrates the surface that head (6) is applied to cell, due to the light beam zone of action cell surface with energy
Gradient force ligh trap is will produce, gradient force ligh trap can limit the spatial position of cell, carry out cell every empty tweezer to reach
Purpose, by the method, from operation ware on organ-tissue every empty tweezer 4 with P 32 cells and 1 without phosphorus
32 cell；
The cartonning of cell operates：By controlling, switch or knob make optical tweezer is worn to penetrate to experimenter accordingly on control panel (2)
Head (6) no longer emits light beam, therefore cell is no longer acted on by the light beam with energy, can then drop into experiment ware, press
This mode carries out 5 operations so that 4 there is P 32 and 1 normal experimental cell to respectively fall in 5 identical experiment wares
In, with carbohydrate, vitamin, amino acid and the trace element needed for cellular physiological events in experiment ware, then manipulate operation control surface
Sealing door switch on plate (2) so that electric sealed door (3) is opened, and then places 4 experimental cells with P 32 respectively
To 4 different temperature cabins in cold-hot type experimental box (1), temperature is respectively 10,20-10 and -20 DEG C, and another is individually
Experiment ware containing normal experimental cell is placed into the environment of room temperature germ-free condition, then closes electric sealed door (3)
It closes；
Cell observes and records：After cell is placed into cold-hot type experimental box (1) by experimenter, after waiting for a couple of days, respectively by 4
The experiment ware of the experiment ware of a experimental cell with P 32 and 1 normal experimental cell, which is placed under electron microscope, to be seen
It examines, experimenter needs whether physiological status, cell membrane and the cytoplasm of observing 5 cells have significant change, and will test
The data of journey and result are recorded.
2. a kind of device for optical tweezer cell and particle to cold and hot formula device, which is characterized in that including cold-hot type experimental box (1)
With spherical laser wave machine (4), the spherical laser wave machine (4) is welded on cold-hot type experimental box (1), the cold-hot type experimental box
(1) control panel (2) has been bolted at the top of, the side of the cold-hot type experimental box (1) is equipped with electric sealed door
(3), concentrating refractive device (5) has been bolted in the bottom of the spherical laser wave machine (4), the concentrating refractive device (5)
Optical tweezer, which has been bolted, in bottom wears and penetrates head (6), and electronic fixator has been bolted on the cold-hot type experimental box (1)
(7), the bottom of the cold-hot type experimental box (1) is welded with self-adjustable fixed seat (10), the side of the cold-hot type experimental box (1)
It is welded with observation panel (9), microcosmic probe (8) has been bolted in the inside of the cold-hot type experimental box (1).
3. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
Optical pressure switch, refraction knob, sealing door switch and fixator switch has been bolted on the control panel (2) respectively,
The interior welds of the control panel (2) have data mainboard, the data mainboard to switch, seal with fixator respectively by electric wire
Door switch, refraction knob and optical pressure switch connection.
4. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
The interior welds of the spherical laser wave machine (4) have optical pressure generator, and the quantity of the optical pressure generator is 2, the optical pressure
Switch is connect by electric wire with optical pressure generator.
5. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
Electronic enocscope has been bolted in the inside of the concentrating refractive device (5), and the quantity of the electronic enocscope is 2, institute
Refraction knob is stated to connect with electronic enocscope by electric wire.
6. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
The cold-hot type experimental box (1) is internally provided with temperature cabin, and the quantity in the temperature cabin is 4, the setting in the temperature cabin
Temperature is respectively 10,20, -10 and -20 DEG C, and the microcosmic probe (8) passes through the data mainboard in data line and control panel (2)
Connection.
7. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
Elevating lever, the top welding of the cold-hot type experimental box (1) has been bolted in the inside of the self-adjustable fixed seat (10)
There are horizon sensor (11), the horizon sensor (11) to be electrically connected by level monitoring circuit and elevating lever.
8. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
It is welded with dual-layer vacuum glass on the observation panel (9).
9. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, it is characterised in that：
The quantity of the microcosmic probe (8) and self-adjustable fixed seat (10) is 4.
10. a kind of device for optical tweezer cell and particle to cold and hot formula device according to claim 2, feature exist
In：The bottom of the cold-hot type experimental box (1) is welded with alarming instrument, and the alarming instrument passes through in electric wire and manipulation plate face (2)
Data mainboard is electrically connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741219.5A CN108795695A (en) | 2018-07-08 | 2018-07-08 | A kind of method and device for optical tweezer cell and particle to cold and hot formula device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741219.5A CN108795695A (en) | 2018-07-08 | 2018-07-08 | A kind of method and device for optical tweezer cell and particle to cold and hot formula device |
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| Publication Number | Publication Date |
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| CN108795695A true CN108795695A (en) | 2018-11-13 |
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| CN201810741219.5A Withdrawn CN108795695A (en) | 2018-07-08 | 2018-07-08 | A kind of method and device for optical tweezer cell and particle to cold and hot formula device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165363A (en) * | 2020-01-19 | 2020-05-19 | 程彩凤 | Radioactive organism experimental box |
-
2018
- 2018-07-08 CN CN201810741219.5A patent/CN108795695A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165363A (en) * | 2020-01-19 | 2020-05-19 | 程彩凤 | Radioactive organism experimental box |
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