CN109136288A - A kind of anaerobic fermentation method of vinegar grain - Google Patents
A kind of anaerobic fermentation method of vinegar grain Download PDFInfo
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- CN109136288A CN109136288A CN201811145382.1A CN201811145382A CN109136288A CN 109136288 A CN109136288 A CN 109136288A CN 201811145382 A CN201811145382 A CN 201811145382A CN 109136288 A CN109136288 A CN 109136288A
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- vinegar grain
- pseudomonas
- medium temperature
- anaerobic fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Treatment Of Sludge (AREA)
Abstract
The invention discloses a kind of anaerobic fermentation method of vinegar grain, method is the following steps are included: (1) addition vinegar grain tames efficient anaerobic activated sludge；(2) using vinegar grain and pig manure hybrid anaerobic fermentation；(3) vinegar grain and pig manure proportion are optimized；(4) increase load step by step under the conditions of optimal proportion, anaerobic fermentation process is carried out with filter residue reflux type.Waste present invention is generally directed to vinegar grain as vinegar industry, there is the problems such as difficult to degrade, acidity is high, efficient anaerobic activated sludge is tamed by addition vinegar grain, hybrid anaerobic fermentation improvement single_phase system is carried out with pig manure simultaneously and is easily acidified problem, the anaerobic digestion process for promoting vinegar grain, makes the operation of fermentation system stability and high efficiency.
Description
Technical field
The invention belongs to agricultural wastes technical field of anaerobic fermentation, and in particular to a kind of anaerobic fermentation method of vinegar grain.
Background technique
Vinegar grain is the fermentation by-product of vinegar product, and national annual output is more than 6,000,000 tons, with high microsteping content
The features such as (25.78% (butt)), lower protein content (12.79% (butt)) and high organic acid content, has certain difficulty
Degradability.These characteristics, which to be easy to appear acid in the homogenous anaerobic fermentation process of vinegar grain, to be inhibited to cause methane phase process by tight
The limitation of weight.
Summary of the invention
For the deficiency of vinegar grain homogenous anaerobic fermentation technique, present invention introduces a kind of anaerobic fermentation methods of vinegar grain, can have
The problem of effect ground stablizes fermentation system, improves methane production.
In order to achieve the above object, the present invention is the following technical schemes are provided: a kind of anaerobic fermentation method of vinegar grain, including following
Step:
(1) proper amount of fresh vinegar grain and methanogen promotor will be added in anaerobic activated sludge, carry out anaerobism under mesophilic condition
Fermentation process is screened and the high-efficiency strain in optimization system with this.The initial TS control 3%~4% of fermentation system, initial pH control
System is 7.3~7.8.Final system methane content reaches 65% or more and tames successfully.
(2) it takes (1) to tame successful anaerobic activated sludge to take out as inoculum, be inoculated in vinegar grain and pig manure mixed system,
Wherein the mass ratio of vinegar grain and pig manure is respectively 1:3,1:1 and 3:1.The VS burden control of system is in pig manure 50%~75%, vinegar
The TS of grain 25%~50%, system is controlled 10%~20%.
(3) according to step (2), it is optimal mixing match with reference to methane production screening 3:1, carries out gradually gradient liter under medium temperature condition
The mode of high load capacity, filter residue reflux carries out the hybrid anaerobic fermentation of vinegar grain and pig manure, and methane content is lower than 55% in fermentation process
When, methane phase promotor is properly added with the stable and high effective operation of guarantee system.
(4) biogas obtained by step (3) passes through gas purification and CO2The processing such as absorption are as natural gas；The addition of gained biogas slurry is appropriate
Element as biological organic fertilizer.
Further, vinegar grain is added by the way of gradually increment in the step (1).
Further, the control of pH is regulated and controled using addition pig manure in the step (1), does not add chemical reagent.
Further, methane promotor is liquid composite biological agent in the step (1) and (3), and main component is to produce
Methane backeria, medium temperature syntrophism acetic acid oxidation bacteria, medium temperature syntrophism propionic acid oxidation bacteria, medium temperature syntrophism butyric acid oxidized bacterium, microelement and dimension
Raw element.Wherein, methanogen include but is not limited to methanimicrococcus, methanococcoides,
methanohalobium、halomethanococcus、methanocella、methanolinea、methanocalculus、
Methanospirillum, methanocorpusculum etc. the Pseudomonas can be used alone or using mixing Pseudomonas.In
Warm syntrophism acetic acid oxidation bacteria is including but not limited to SAOB Clostridium ultunense, Thermacetogenium
Phaeum, Tepidanaerobacter acetatoxydans Re1, Thermoanaerobacteraceae and
Thermotoga lettingae etc. the Pseudomonas can be used alone or using mixing Pseudomonas.The oxidation of medium temperature syntrophism propionic acid
Bacterium including but not limited to Smithella propionica, Syntrophobacter fumaroxidans and
Pelotomaculum thermopropionicum etc. the Pseudomonas can be used alone or using mixing Pseudomonas.Medium temperature is mutual
Seek butyric acid oxidized bacterium including but not limited to Syntrophomonas wolfei subsp.Methylbutyratica subsp,
Syntrophomonas cellicola、Syntrophomonas erecta、Syntrophomonas erecta
Subsp.sporosyntropha subsp, Syntrophomonas sapovorans and Syntrophomonas
Palpitatica etc. the Pseudomonas can be used alone or using mixing Pseudomonas.Microelement be iron content (Fe), cobalt (Co),
Nickel (Ni), molybdenum (Mo), tungsten (W) and selenium (Se) compound, including but do not limit to ferrous sulfate, cobalt chloride, nickel chloride, molybdic acid
Sodium, sodium tungstate and sodium selenate.Vitamin is containing niacin, p-aminobenzoic acid, vitamin B6 (including but not limited to pyridoxine), dimension
Raw element B7 (including but not limited to biotin), folic acid, vitamin B2 (including but not limited to riboflavin), thiamine.
Further, temperature is 35 ± 2 DEG C in the step (1) and (3).
Further, anaerobic fermentation effective volume is 70%~80% in the step (3).
The invention has the benefit that
(1) anaerobic activated sludge wherein microorganism will can self eliminate by the new fresh vinegar grain of addition and methane phase promotor,
To filter out can efficient degradation vinegar grain hydrolysis acidification bacterium, while the methanogen in system is strengthened, is made in system
Microbiologic population reach the state of stable equilibrium.
(2) vinegar grain itself peracid can be carried out advantageous neutralization, reach the cushion performance in system by vinegar grain and pig manure mixed fermentation
To higher level, alleviate because of system acid suppression caused by hyper acid.Optimal mixing match is filtered out simultaneously, in the hope of reaching
System best nutritional component ratio is more conducive to the metabolism and growth of microorganism, to promote the yield of methane.
(3) by gradient increasing productivity step by step, the mode of filter residue reflux carries out hybrid anaerobic fermentation, on the one hand can avoid due to one
On the other hand excess load unstability caused by secondary property charging is excessive can allow microorganism gradually to adapt to turn from load to high load capacity
Become；Secondly, filter residue reflux both can block pipeline to avoid due to excessive biogas residue, while reflux can reduce microorganism loss.
(4) reach busy hour, repeatedly a small amount of addition methane phase promotor, can maintain the stable operation of fermentation system, simultaneously
Methane production can be improved.
Specific embodiment
A specific embodiment of the invention is described below, protection scope of the present invention is not limited to as described below.
Embodiment 1
(1) after mixing evenly by new fresh vinegar grain, it is added in anaerobic activated sludge, additive amount load is 0.5g VS/L.d；Together
The a small amount of methane phase promotor of Shi Tianjia, additive amount 40mL/L are primary every addition in three days.It tames to 55d~60d, system first
Alkane content is 67%~70%.
(2) inoculum for taming successful activated sludge as vinegar grain anaerobic fermentation is taken, is inoculated in vinegar grain and pig manure mixture,
Inoculative proportion is 50%.Vinegar grain and the mass mixing ratio of pig manure are 1:3,1:1 and 3:1, are carried out under the conditions of 35 ± 2 DEG C of medium temperature
The anaerobic fermentation of 60d by a definite date.Period, measurement methane content are above 65%, but gas production difference.
(3) by methane production under the conditions of each ratio of comparison, when vinegar grain and pig manure ratio are 1:3, methane production is maximum, compared with 1:1
10%~15% is improved with 3:1, therefore screens optimum proportioning of this ratio as vinegar grain and pig manure hybrid anaerobic fermentation.
Embodiment 2
(1) vinegar grain is mixed with pig manure according to mass ratio 1:3, load is 1.0~6.0g VS/L.d, with 1.0g VS/
The rate of L.d is slowly incremented by, cycle of operation 30d, hydraulic detention time 20d, carries out 35 ± 2 DEG C of medium temperature of anaerobic fermentation.
Period feeds identical as discharging volume daily, and discharging filters out solid residue by strainer, in liquid reflux to fermentor.
(2) when load reaches 6.0g VS/L.d, the methane content of system drops to 55% hereinafter, addition while charging
Methane phase promotor, additive amount 10mL/L, adds 4 times, adds every other day for one day.When methane content restores to 60% or more, stop
Only add promotor.
Comparative example 1
(1) compared with example 1, step (1) does not add vinegar grain and methane phase promotor.
(2) compared with example 1, step (2) does not add pig manure, only adds vinegar grain as single fermentation raw material.
Comparative example 2
(1) compared with example 2, step (1) feed way be load by 1.0g VS/L.d, with 3.0g VS/L.d rate
Addition only adds single vinegar grain material, while without reflux operation.
(2) compared with example 2, step (2) methane content be lower than 55% when do not take addition methane phase promotor, only carry out into
Material operation.
(3) remaining operating process and example 2 are consistent.
Validity check
Detection example 1 and comparative example 1, the methane content of 1 output of example improve about 15% compared with comparative example 1 under the same terms,
The starting period of anaerobic fermentation, example 1 are advanced by 3 days compared with comparative example 1 simultaneously.
Detection example 2 and comparative example 2,2 peak load of example can run to 6.0g VS/L.d, contain in methane under the same terms
When amount is lower than 55%, promotor is added, methane content restores rapidly to 60% or more, and fermentor keeps operating normally；Comparative example 2
Peak load is only run to 2.5g VS/L.d, during continuous charging, does not add promotor, methane content reduces rapidly
To 30% hereinafter, and stopping producing gas in 10d.
The present invention can effectively improve vinegar grain utilization rate and methane phase efficiency, while can be effectively relieved for this special material of vinegar grain
It, which is acidified, inhibits.
Claims (5)
1. a kind of anaerobic fermentation method of vinegar grain, it is characterised in that:
Proper amount of fresh vinegar grain and methanogen promotor will be added in anaerobic activated sludge, carry out anaerobism hair under mesophilic condition
Ferment process is screened and the high-efficiency strain in optimization system with this.The initial TS control 3%~5% of fermentation system, initial pH control
7.3~7.8.Final system methane content reaches 65% or more and tames successfully.
2. the successful anaerobic activated sludge of domestication is taken to take out as inoculum, it is inoculated in vinegar grain and pig manure mixed system, screening
Mass ratio is that 1:3 is optimal mixing match, and gradually gradient is carried out under the conditions of 35 ± 2 DEG C of medium temperature and increases load, the side of filter residue reflux
Formula carries out the hybrid anaerobic fermentation of vinegar grain and pig manure, when fermentation process methane content is lower than 55%, is properly added methane phase promotion
Agent is with the stable and high effective operation of guarantee system.
3. gained biogas passes through gas purification and CO2The processing such as absorption are as natural gas；Gained biogas slurry adds suitable element conduct
Biological organic fertilizer.
4. further, vinegar grain is added by the way of gradually increment in the step 1；The control of pH is using addition pig
Excrement is regulated and controled, and chemical reagent is not added.
5. further, methane promotor is liquid composite biological agent in the step 1 and 2, main component is methanogen,
Medium temperature syntrophism acetic acid oxidation bacteria, medium temperature syntrophism propionic acid oxidation bacteria, medium temperature syntrophism butyric acid oxidized bacterium, microelement and vitamin.Its
In, methanogen include but is not limited to methanimicrococcus, methanococcoides, methanohalobium,
halomethanococcus、methanocella、methanolinea、methanocalculus、methanospirillum、
Methanocorpusculum etc. the Pseudomonas can be used alone or using mixing Pseudomonas.Medium temperature syntrophism acetic acid oxidation bacteria
Including but not limited to SAOB Clostridium ultunense, Thermacetogenium phaeum,
Tepidanaerobacter acetatoxydans Re1, Thermoanaerobacteraceae and Thermotoga
Lettingae etc. the Pseudomonas can be used alone or using mixing Pseudomonas.Medium temperature syntrophism propionic acid oxidation bacteria includes but unlimited
In Smithella propionica, Syntrophobacter fumaroxidans and Pelotomaculum
Thermopropionicum etc. the Pseudomonas can be used alone or using mixing Pseudomonas.Medium temperature syntrophism butyric acid oxidized bacterium bag
Contain but is not limited to Syntrophomonas wolfei subsp.Methylbutyratica subsp, Syntrophomonas
cellicola、Syntrophomonas erecta、Syntrophomonas erecta subsp.sporosyntropha
Subsp, Syntrophomonas sapovorans and Syntrophomonas palpitatica etc., can be single by the Pseudomonas
Solely using or using mixing Pseudomonas.Microelement is iron content (Fe), cobalt (Co), nickel (Ni), molybdenum (Mo), tungsten (W) and selenium (Se)
Compound, including but do not limit to ferrous sulfate, cobalt chloride, nickel chloride, sodium molybdate, sodium tungstate and sodium selenate.Vitamin be containing
Niacin, p-aminobenzoic acid, vitamin B6 (including but not limited to pyridoxine), vitamin B7 (including but not limited to biotin),
Folic acid, vitamin B2 (including but not limited to riboflavin), thiamine.
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| CN201811145382.1A CN109136288A (en) | 2018-09-29 | 2018-09-29 | A kind of anaerobic fermentation method of vinegar grain |
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| CN201811145382.1A CN109136288A (en) | 2018-09-29 | 2018-09-29 | A kind of anaerobic fermentation method of vinegar grain |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330090A (en) * | 2018-03-13 | 2018-07-27 | 常州大学 | For the preparation method and purposes of the composite biological agent of pig manure waste medium temperature biogas fermentation high-content volatile fatty acid |
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- 2018-09-29 CN CN201811145382.1A patent/CN109136288A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330090A (en) * | 2018-03-13 | 2018-07-27 | 常州大学 | For the preparation method and purposes of the composite biological agent of pig manure waste medium temperature biogas fermentation high-content volatile fatty acid |
Non-Patent Citations (4)
| Title |
|---|
| GENZHU RAN，ET AL: "Hydrothermal pretreatment on the anaerobic digestion of wasted vinegar residue", 《BIORESOURCE TECHNOLOGY》 * |
| 刘瑞光: "醋糟厌氧发酵生物制氢试验研究", 《中国优秀硕士学位论文全文数据库》 * |
| 赵士永 等: "《村镇绿色小康住宅技术集成》", 31 March 2017, 中国轻工业出版社 * |
| 陈晓寅 等: "醋糟的利用现状及前景", 《中国酿造》 * |
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