CN109134465A - A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation - Google Patents
A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation Download PDFInfo
- Publication number
- CN109134465A CN109134465A CN201811047559.4A CN201811047559A CN109134465A CN 109134465 A CN109134465 A CN 109134465A CN 201811047559 A CN201811047559 A CN 201811047559A CN 109134465 A CN109134465 A CN 109134465A
- Authority
- CN
- China
- Prior art keywords
- hairy root
- salvia miltiorrhiza
- accumulation
- tanshinone
- root cultures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/12—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains three hetero rings
- C07D471/18—Bridged systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed systems contains four or more hetero rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/04—Ortho-condensed systems
- C07D491/044—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
- C07D491/048—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring the oxygen-containing ring being five-membered
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of alkaloid and the applications in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation.It is a discovery of the invention that Mei Luo rather B, Mei Luo rather C, Mei Luo rather G, Mei Luo rather can significantly can can can induce the accumulation of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza by H, and to the accumulation of tanshinone without obvious effect；On the contrary, Mei Luo rather J, Leuconolam can significantly can induce the accumulation of tanshinone in Hairy Root Cultures of Salvia miltiorrhiza, and to the accumulation of danshinolic acid without obvious effect；But, Mei Luo rather can not only can induce the accumulation of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza by A, but also can induce the accumulation of tanshinone in Hairy Root Cultures of Salvia miltiorrhiza.
Description
Technical field
The invention belongs to chemical fields, are related to a kind of alkaloid and danshinolic acid and tanshinone product in induction Hairy Root Cultures of Salvia miltiorrhiza
The application of tired aspect.
Background technique
Radix Salviae Miltiorrhizae is also known as red ginseng, clovershrub, is one of main bulk medicinal materials in China.Radix Salviae Miltiorrhizae has promoting blood circulation, and promoting menstruation stops
Bitterly, the effect of relieving restlessness and restlessness, cool blood to disappear carbuncle, can be used for dysphoria and insomnia, irregular menstruation, the treatment of sore swell and ache curative etc..Currently, Radix Salviae Miltiorrhizae
Demand rise year by year.But the variet complexity of country's Radix Salviae Miltiorrhizae, germplasm are degenerated seriously at present, and active constituent content is extremely unstable,
Have become red rooted salvia industrialized development and moves towards the bottleneck of international market.In recent years, people utilize artificial organ and cell
The method substitution Radix Salviae Miltiorrhizae plant of culture made breakthrough progress to produce various effective components.Wherein Hairy Root Cultures of Salvia miltiorrhiza is with it
The speed of growth is fast, stabilization characteristics of genetics, and culture medium does not need the advantages that adding hormone and is gradually taken seriously.But it accumulates Radix Salviae Miltiorrhizae
Effective component especially danshinolic acid, tanshinone component ability be unable to reach always crude drug level.
Therefore, it is necessary to develop the inducer of danshinolic acid in induction Hairy Root Cultures of Salvia miltiorrhiza, tanshinone accumulation.
Summary of the invention
The present invention is intended to provide a kind of alkaloid and in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation
Using.
The object of the invention is realized by following technical solution:
A kind of alkaloid, chemical structural formula are as follows:
Above-mentioned alkaloid is used as the purposes of the inducer of danshinolic acid accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza.
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
A kind of culture medium for inducing danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza to accumulate, contains above-mentioned alkaloid and other routines
Ingredient etc..
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
A kind of alkaloid, chemical structural formula are as follows:
Above-mentioned alkaloid is used as the purposes of the inducer of danshinolic acid accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza.
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
A kind of culture medium for inducing danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza to accumulate, contains above-mentioned alkaloid and other routines
Ingredient etc..
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
A kind of alkaloid, chemical structural formula are as follows:
Above-mentioned alkaloid is used as the purposes of the inducer of tanshinone accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza.
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
A kind of culture medium for inducing tanshinone in Hairy Root Cultures of Salvia miltiorrhiza to accumulate, contains above-mentioned alkaloid and other routines
Ingredient etc..
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
A kind of alkaloid, chemical structural formula are as follows:
Above-mentioned alkaloid is used as the purposes of the inducer of tanshinone accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza.
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
A kind of culture medium for inducing tanshinone in Hairy Root Cultures of Salvia miltiorrhiza to accumulate, contains above-mentioned alkaloid and other routines
Ingredient etc..
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
A kind of alkaloid, chemical structural formula are as follows:
Above-mentioned alkaloid is used as the purposes of danshinolic acid or the inducer of tanshinone accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza.
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
It is a kind of for inducing the culture medium of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza or tanshinone accumulation, containing above-mentioned alkaloid, with
And other conventional ingredients etc..
Preferably, the danshinolic acid is tanshin polyphenolic acid B.
Preferably, the tanshinone is dihydrotanshinone Ⅰ.
The utility model has the advantages that
It is a discovery of the invention that Mei Luo rather B, Mei Luo rather C, Mei Luo rather G, Mei Luo rather can significantly can can can induce Radix Salviae Miltiorrhizae hair by H
The accumulation of danshinolic acid in shape root, and to the accumulation of tanshinone without obvious effect；On the contrary, Mei Luo can rather J, Leuconolam can be shown
The accumulation of tanshinone in induction Hairy Root Cultures of Salvia miltiorrhiza is write, and to the accumulation of danshinolic acid without obvious effect；But, Mei Luo can rather A both may be used
To induce the accumulation of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza, and the accumulation of tanshinone in Hairy Root Cultures of Salvia miltiorrhiza can be induced.
Detailed description of the invention
Fig. 1 is the relative amount of tanshin polyphenolic acid B and dihydrotanshinone Ⅰ in the hairy root timber material of each group.
Specific embodiment
Introduce essentiality content of the invention in detail with reference to the accompanying drawings and examples, but those skilled in the art should
Know, is not limited the scope of protection of the present invention with this.
One, experimental material
The chemical structure for the alkaloid that the embodiment uses is as shown in the table, and purity is not less than 95%.
This experiment is to infect Radix Salviae Miltiorrhizae aseptic seedling by agrobacterium rhizogenes LBA9402 to obtain with Hairy Root Cultures of Salvia miltiorrhiza, and in MSoH liquid
25 DEG C of darkroom squamous subcultures of body culture medium.
Two, experimental method
1, the culture of Hairy Root Cultures of Salvia miltiorrhiza
This experiment is to infect Radix Salviae Miltiorrhizae aseptic seedling by agrobacterium rhizogenes LBA9402 to obtain with Hairy Root Cultures of Salvia miltiorrhiza, and in MSoH liquid
25 DEG C of darkroom squamous subcultures of body culture medium.MSoH culture medium refers on the basis of Murashige and Skoog (MS) culture medium
On by 1g/L caseinhydrolysate substitute NH4NO3(bibliography: Hu ZB, AlfermannAW.Diterpenoidproduction
in hairy root Cultures ofSalviamiltiorrhiza.Phytochemistry,1993)。
2, the preparation of inducer mother liquor
Using DMSO as cosolvent, by Mei Luo can peaceful B, Mei Luo can peaceful C, Mei Luo can peaceful G, Mei Luo can peaceful H, Mei Luo can peaceful J,
Leuconolam, Mei Luo rather can use deionized water dissolving by A respectively, be configured to the mother liquor that concentration is 100mM, spare.
3, Hairy Root Cultures of Salvia miltiorrhiza induction is handled
The Hairy Root Cultures of Salvia miltiorrhiza of 1g inoculation experiments room conservation is being cultivated after 18d in 100ml MSOH fluid nutrient medium
Addition U.S. Lip river can peaceful B, Mei Luo can peaceful C, Mei Luo can peaceful G, Mei Luo can rather H, Mei Luo can peaceful J, Leuconolam respectively in hairy
Or Mei Luo can peaceful A carry out induction processing, Mei Luo can peaceful B, Mei Luo can peaceful C, Mei Luo can peaceful G, Mei Luo can peaceful H, Mei Luo can peaceful J,
Leuconolam or Mei Luo can the final concentration of peaceful A be respectively 100 μM；Blank control group is set simultaneously.Each processing is repeated 3 times,
It collects the hairy root timber material after inducing processing 10d and is freezed in -80 DEG C of refrigerators, be subsequently placed in freeze drier and be lyophilized, and
It is stored in spare in electronic dry device.
4, the extraction of Hairy Root Cultures of Salvia miltiorrhiza effective component
The Hairy Root Cultures of Salvia miltiorrhiza grind into powder of freeze-drying is taken, 40 meshes are crossed, precision weighs sample 0.05g in 10mL centrifuge tube
In, add 70% methanol ultrasonic extraction 45min to supply original volume, 12000r/min is centrifuged 10min, and supernatant is filtered through 0.22 μm of micropore
Film filters to get sample extracting solution.
5, in hairy root timber material tanshin polyphenolic acid B and dihydrotanshinone Ⅰ assay
Using document HPLC method, (Zhang Shuncang etc., elicitor accumulate Hairy Root Cultures of Salvia miltiorrhiza phenolic acid class and tanshinone component
Influence, CHINA JOURNAL OF CHINESE MATERIA MEDICA, in May, 2011) measurement the hairy root timber material of each group in tanshin polyphenolic acid B and dihydrotanshinone Ⅰ content,
Then the relative amount of each induction group is obtained divided by the content of blank control group with the content of induction group.
6, data statistics
It is handled using SPSS 17.0, group difference uses one-way analysis of variance method, and P < 0.05 is that difference has statistics
Meaning.
Three, experimental result
The relative amount of tanshin polyphenolic acid B and dihydrotanshinone Ⅰ is as shown in table 1 and Fig. 1 in the hairy root timber material of each group.
The relative amount of tanshin polyphenolic acid B and dihydrotanshinone Ⅰ in the hairy root timber material of 1 each group of table
The above results show that Mei Luo rather B, Mei Luo rather C, Mei Luo rather G, Mei Luo rather can significantly can can can induce Radix Salviae Miltiorrhizae by H
The accumulation of hairy middle danshinolic acid, and to the accumulation of tanshinone without obvious effect；On the contrary, Mei Luo can peaceful J, Leuconolam can be with
The accumulation of tanshinone in significant induction Hairy Root Cultures of Salvia miltiorrhiza, and to the accumulation of danshinolic acid without obvious effect；But, Mei Luo can peaceful A both
The accumulation of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza can be induced, and the accumulation of tanshinone in Hairy Root Cultures of Salvia miltiorrhiza can be induced.
One skilled in the art will appreciate that chemical composition from plant is accumulative related to relevant gene, expression of enzymes level, and
The expression of these genes, enzyme is influenced by extraneous chemical component again, and this influence may be activation, it is also possible to inhibit, because
This, as long as screening these genes, specified chemical ingredient in effective activator of enzyme or inhibitor human intervention plant
Accumulation.
The effect of above-described embodiment, which is only in that, specifically introduces essentiality content of the invention, but those skilled in the art
It should be known that protection scope of the present invention should not be limited only to above-mentioned specific embodiment.
Claims (7)
1. a kind of alkaloid, which is characterized in that chemical structural formula is as follows:
2. the use that alkaloid described in claim 1 is used as danshinolic acid or the inducer of tanshinone accumulation in induction Hairy Root Cultures of Salvia miltiorrhiza
On the way.
3. purposes according to claim 2, it is characterised in that: the danshinolic acid is tanshin polyphenolic acid B.
4. purposes according to claim 2, it is characterised in that: the tanshinone is dihydrotanshinone Ⅰ.
5. a kind of for inducing the culture medium of danshinolic acid in Hairy Root Cultures of Salvia miltiorrhiza or tanshinone accumulation, it is characterised in that: containing having the right
It is required that alkaloid described in 1 and other conventional ingredients etc..
6. culture medium according to claim 5, it is characterised in that: the danshinolic acid is tanshin polyphenolic acid B.
7. culture medium according to claim 5, it is characterised in that: the tanshinone is dihydrotanshinone Ⅰ.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811047559.4A CN109134465A (en) | 2018-09-10 | 2018-09-10 | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811047559.4A CN109134465A (en) | 2018-09-10 | 2018-09-10 | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109134465A true CN109134465A (en) | 2019-01-04 |
Family
ID=64824155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811047559.4A Withdrawn CN109134465A (en) | 2018-09-10 | 2018-09-10 | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109134465A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110090216A (en) * | 2019-04-28 | 2019-08-06 | 山东省分析测试中心 | The application of indole alkaloids compound and its derivative or salt in prevention and treatment diabetic nephropathy product |
-
2018
- 2018-09-10 CN CN201811047559.4A patent/CN109134465A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110090216A (en) * | 2019-04-28 | 2019-08-06 | 山东省分析测试中心 | The application of indole alkaloids compound and its derivative or salt in prevention and treatment diabetic nephropathy product |
| CN110090216B (en) * | 2019-04-28 | 2021-07-16 | 山东省分析测试中心 | Application of indole alkaloid compound and derivatives or salts thereof in products for preventing and treating diabetic nephropathy |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100453094C (en) | Medicine composition of effective parts for compound Tongmai Chinese medicine oral liquid and its preparing method | |
| CN106236808B (en) | A kind of stevia rebaudiana phenol extract and its application in anti-inflammatory product | |
| CN101293012B (en) | Antiviral honeysuckle flower Chinese medicine compound preparation and preparation technique | |
| CN101732669B (en) | Traditional Chinese medicine used for treating gynecologic inflammation, preparation method and application thereof | |
| CN102961394A (en) | Application of geniposide in preparation of medicine for treating fatty liver disease | |
| CN109432315A (en) | Application of the cogongrass rhizome extractive as active constituent in the product that preparation relieves fatigue | |
| CN109134465A (en) | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid and tanshinone accumulation | |
| CN102370635B (en) | Application and preparation method of novel nardosinone | |
| CN105037464B (en) | A kind of setose thistle flavone compound and preparation method thereof and the application in antitumor or liver-protecting medicine is prepared | |
| CN109134464A (en) | A kind of alkaloid and its purposes as tanshinone accumulation inducer in Hairy Root Cultures of Salvia miltiorrhiza | |
| CN104873560B (en) | A kind of setose thistle general flavone and preparation method thereof with preparing the application in antitumor or liver-protecting medicine | |
| CN109134466A (en) | A kind of alkaloid and its purposes as danshinolic acid accumulation inducer in Hairy Root Cultures of Salvia miltiorrhiza | |
| KR20150014033A (en) | Food composition for liver activity contaning Water extract of Cordyceps militaris and its manufacturing method | |
| CN104971088A (en) | Tibetan artemisia capillaris extract and preparation method, drug composition and application thereof | |
| CN102552369B (en) | Lon icera japonica Thunb leaf extractive serving as anti-porcine reproductive and respiratory syndrome viral agent | |
| CN104472363A (en) | Induction method for promoting accumulation of rosmarinic acid and danshinolic acid in salvia miltiorrhiza hairy roots | |
| CN108997348A (en) | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of tanshinone accumulation | |
| CN109053733A (en) | A kind of alkaloid and the application in induction Hairy Root Cultures of Salvia miltiorrhiza in terms of danshinolic acid accumulation | |
| CN104644611A (en) | Method for preparing microcapsules | |
| CN103285082B (en) | Medicament composition for treating gynecological inflammation | |
| CN101263811B (en) | Method for improving yield of glycyrrhizae hairy root secondary metabolism product | |
| CN104042647B (en) | The application in preparing the liver protecting medicine of the Herba Ardisiae Japonicae total flavones | |
| JP7150142B2 (en) | Spray-drying method for broad-cut granules | |
| CN106177035B (en) | Preparation method and application of effective rosa chinensis flower extract with blood sugar reducing and anticancer functions | |
| CN105646164A (en) | Neolignan compound for the treatment of gastric cancer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190104 |