CN109130293A - The tablet forming technique and its production technology of sheet cell culture medium - Google Patents
The tablet forming technique and its production technology of sheet cell culture medium Download PDFInfo
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- CN109130293A CN109130293A CN201810875072.9A CN201810875072A CN109130293A CN 109130293 A CN109130293 A CN 109130293A CN 201810875072 A CN201810875072 A CN 201810875072A CN 109130293 A CN109130293 A CN 109130293A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B30—PRESSES
- B30B—PRESSES IN GENERAL
- B30B11/00—Presses specially adapted for forming shaped articles from material in particulate or plastic state, e.g. briquetting presses, tabletting presses
- B30B11/02—Presses specially adapted for forming shaped articles from material in particulate or plastic state, e.g. briquetting presses, tabletting presses using a ram exerting pressure on the material in a moulding space
- B30B11/04—Presses specially adapted for forming shaped articles from material in particulate or plastic state, e.g. briquetting presses, tabletting presses using a ram exerting pressure on the material in a moulding space co-operating with a fixed mould
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B30—PRESSES
- B30B—PRESSES IN GENERAL
- B30B15/00—Details of, or accessories for, presses; Auxiliary measures in connection with pressing
- B30B15/06—Platens or press rams
- B30B15/065—Press rams
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B30—PRESSES
- B30B—PRESSES IN GENERAL
- B30B15/00—Details of, or accessories for, presses; Auxiliary measures in connection with pressing
- B30B15/08—Accessory tools, e.g. knives; Mountings therefor
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Abstract
A kind of tablet forming technique and its production technology of sheet cell culture medium, wherein, the tablet forming technique of the sheet cell culture medium, include the following steps: (S41), one tablet press machine of selection, the material at the formed punch position of the upper punching of tablet press machine is non-stick substance, and the material at the formed punch position of the undershoot of tablet press machine is non-stick substance；(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；(S43), using feeder into middle die hole tytosis culture medium dry powder；(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole, and the scheduled stroke of downlink, and the cell culture medium dry powder in middle die hole is pressed into cell culture substrate；(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；(S46), undershoot rises die hole in the ejection of cell culture substrate, completes tableting processes with this.The present invention has the advantages that yield is higher, precision is higher, process is simplified, easy to control.
Description
Technical field
The present invention relates to the production technology field of cell culture medium, especially a kind of life applied to sheet cell culture medium
Production. art.
Background technique
Cell culture medium is according to zooblast growth and breeding or to accumulate various nutriments required for metabolite, by
The nutrient matrix being manually formulated specializes in animal cell culture, separation identification, research and saves use, to meet cell growth
With the needs of metabolism, culture medium must include carbon source, nitrogen source, water, six major class nutriment of inorganic salts, the energy and growth factor.
And cell culture medium is both to cultivate supply cytotrophy and the basic substance for promoting germiparity to be proliferated in cell, and culture is thin
The living environment of intracellular growth and breeding.
The one kind of dehydrated medium as culture medium, be by the various composition of culture medium by premixing, grinding and after mix
The processing appropriate such as conjunction, mix well into powdered according to needed for the formula of various culture mediums.Taken when use this powder by than
Example plus distilled water dissolve packing sterilizing.
Although dehydrated medium can make culture medium reach standardization, the manufacturing time of culture medium is saved, dry powder training
Feeding base still has following problems:
(1) the high problem of dust；The particle of dehydrated medium is 70-150 microns general, so user is weighing, transport,
It is easy to produce dust during preparation, danger is generated to user's health, and the dust generated improves user's production
The cleaning difficulty at scene, to promote cross contamination when user's production or experiment.
(2) the long problem of dissolution time；Dehydrated medium can float on liquid during dissolution, when leading to dissolution
Between it is partially long.
(3) problem of fluid nutrient medium time length is prepared；Quantitative, required time must be weighed when dehydrated medium use
It is long, and the risk of mistake weighing and the risk of cross contamination are easy to happen during weighing.
To solve the above-mentioned problems, someone comes up with the method that cell culture medium dry powder is carried out tabletting, still, due to thin
Born of the same parents' broth viscosity is high, more special, and cell culture medium dry powder can adhere to formed punch part in the production of general tabletting method, such as
This one will destroy cell culture substrate, keep the cell culture substrate of production imperfect.
Summary of the invention
In order to overcome the above problem, the present invention provides that a kind of yield is higher, precision is higher, process is simplified, control to society
The tablet forming technique of convenient sheet culture medium.
The present invention also provides the sheet cell training that a kind of yield is higher, precision is higher, process is simplified, easy to control to society
Support the production technology of base.
The technical scheme is that providing a kind of tablet forming technique of sheet cell culture medium, include the following steps:
(S41), a tablet press machine, the material for the contact layer that the formed punch position of the upper punching of tablet press machine is used to contact with tabletting are selected
It is non-stick substance, the material of the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-goo
Matter；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, by middle mould
Cell culture medium dry powder in hole is pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
As improvement of the present invention, the contact layer at the formed punch position of the upper punching is arranged by way of package,
The contact layer at the formed punch position of the undershoot is arranged by way of package.
As improvement of the present invention, the upper punching is made of non-stick substance, and the undershoot is by non-sticky object
Made of matter.
As improvement of the present invention, the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE)
(PTFE) 10-20 parts, 15-30 parts of anodised aluminium, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
As improvement of the present invention, the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE)
(PTFE) 14-16 parts, 20-25 parts of anodised aluminium, 15-20 parts of ceramics, 10-15 parts of silica gel, 20-30 parts of enamelled cast iron.
Another technical solution of the invention is: a kind of production technology of sheet cell culture medium is provided,
(S1), it is pre-mixed；It is 20-40 parts sugared by 0-30 parts of amino acid 1,30-70 parts of salt, 10-20 parts of buffer mixing,
Temperature is 5-40 DEG C, under conditions of humidity is less than 50%, powder is layered diffusion movement by diffusion blender device, in outer masterpiece
It is moved in newborn level with lower isolated powder, so that each component powder is spread realization in subrange and be uniformly distributed, 0.5-
Premix is formed after 10h；
(S2), it grinds；Above-mentioned premix is put into beveller, maintains the temperature at 4-80 DEG C, spin finishing instrument is to pre-
Mixture is ground, and the granular size that end product is made is 50-150 μm；
(S3), rear mixing；By smashed culture medium dry powder at 5-40 DEG C, humidity be less than 50% under conditions of, pass through
Powder is layered diffusion movement by diffusion blender device, and the powder separated under external force is moved in newborn level, makes each group
Divide powder to spread realization in subrange to be uniformly distributed, needs 0.5-10h；
(S4), tabletting:
(S41), a tablet press machine, the material of the contact layer for contacting with tabletting at the formed punch position of the upper punching of tablet press machine are selected
Matter is non-stick substance, and the material of the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-goo
Matter；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, by middle mould
Cell culture medium dry powder in hole is pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
As improvement of the present invention, the contact layer at the formed punch position of the upper punching is arranged by way of package,
The contact layer at the formed punch position of the undershoot is arranged by way of package.
As improvement of the present invention, the upper punching is made of non-stick substance, and the undershoot is by non-sticky object
Made of matter.
As improvement of the present invention, the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE)
(PTFE) 10-20 parts, 15-30 parts of anodised aluminium, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
As improvement of the present invention, the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE)
(PTFE) 14-16 parts, 20-25 parts of anodised aluminium, 15-20 parts of ceramics, 10-15 parts of silica gel, 20-30 parts of enamelled cast iron.
The present invention is non-sticky due to the material for the contact layer that the formed punch position of the upper punching of tablet press machine is used to contact with tabletting
The material of substance, the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-stick substance；Therefore,
(1) particle of the sheet culture medium generated using tablet forming technique of the invention is in the range of 1mm-25cm, so user exists
It weighs, transport does not generate dust during preparation, lower danger to user's health, and lower user's production or real
Cross contamination when testing；(2) sheet culture medium can be sunk in liquid during dissolution, reduce dissolution time；(3) sheet
Culture medium using when be not required to weigh quantitative, required the preparations time is short, thus lower weigh during be easy to send outRaw mistake weighs
With the risk of cross contamination.Most of all, utilizing sheet culture medium, cell culture medium made by tablet forming technique of the invention
Dry powder does not stick to formed punch part in tableting processes, and the non-stick substance will not cause shadow to the performance of sheet culture medium
It rings, just can thus protect cell culture substrate, keep the cell culture substrate of production more complete, in this way, produce
Yield it is then higher, precision is also higher, operational sequence also more simplify.Therefore, the present invention have can, precision higher to yield more
High, process simplification, advantage easy to control.
Detailed description of the invention
Fig. 1 is the flow chart of the sheet cell culture medium production technology in the present invention.
Specific embodiment
In the present invention, a kind of tablet forming technique of sheet cell culture medium includes the following steps:
(S41), a tablet press machine, the material for the contact layer that the formed punch position of the upper punching of tablet press machine is used to contact with tabletting are selected
It is non-stick substance, the material of the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-goo
Matter；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, by middle mould
Cell culture medium dry powder in hole is pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
In the present invention, it is preferred that the contact layer at the formed punch position of the upper punching is arranged by way of package, described
The contact layer at the formed punch position of undershoot is arranged by way of package.
In the present invention, it is preferred that the upper punching is made of non-stick substance, and the undershoot is by non-stick substance system
At.
In the present invention, it is preferred that the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE) (PTFE)
10-20 parts, 15-30 parts of anodised aluminium, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
In the present invention, it is preferred that the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE) (PTFE)
14-16 parts, 20-25 parts of anodised aluminium, 15-20 parts of ceramics, 10-15 parts of silica gel, 20-30 parts of enamelled cast iron.
Specifically, in the present invention, the non-stick substance has following embodiments:
Embodiment 1
The non-stick substance contains following components in parts by weight: 10 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium 15
Part, ceramic 5 parts, 5 parts of silica gel, 10 parts of enamelled cast iron.
Embodiment 2
The non-stick substance contains following components in parts by weight: 14 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium 20
Part, ceramic 15 parts, 10 parts of silica gel, 20 parts of enamelled cast iron.
Embodiment 3
The non-stick substance contains following components in parts by weight: 16 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium 25
Part, ceramic 20 parts, 15 parts of silica gel, 30 parts of enamelled cast iron.
Embodiment 4
The non-stick substance contains following components in parts by weight: 20 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium 30
Part, ceramic 30 parts, 20 parts of silica gel, 40 parts of enamelled cast iron.
The present invention also provides a kind of production technology of sheet cell culture medium,
(S1), it is pre-mixed；It is 20-40 parts sugared by 0-30 parts of amino acid 1,30-70 parts of salt, 10-20 parts of buffer mixing,
Temperature is 5-40 DEG C, under conditions of humidity is less than 50%, powder is layered diffusion movement by diffusion blender device, in outer masterpiece
It is moved in newborn level with lower isolated powder, so that each component powder is spread realization in subrange and be uniformly distributed, 0.5-
Premix is formed after 10h；
(S2), it grinds；Above-mentioned premix is put into beveller, maintains the temperature at 4-80 DEG C, spin finishing instrument is to pre-
Mixture is ground, and the granular size that end product is made is 50-150 μm；
(S3), rear mixing；By smashed culture medium dry powder at 5-40 DEG C, humidity be less than 50% under conditions of, pass through
Powder is layered diffusion movement by diffusion blender device, and the powder separated under external force is moved in newborn level, makes each group
Divide powder to spread realization in subrange to be uniformly distributed, needs 0.5-10h；
(S4), tabletting:
(S41), a tablet press machine, the material of the contact layer for contacting with tabletting at the formed punch position of the upper punching of tablet press machine are selected
Matter is non-stick substance, and the material of the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-goo
Matter；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, by middle mould
Cell culture medium dry powder in hole is pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
In the present invention, it is preferred that the contact layer at the formed punch position of the upper punching is arranged by way of package, described
The contact layer at the formed punch position of undershoot is arranged by way of package.
In the present invention, it is preferred that the upper punching is made of non-stick substance, and the undershoot is by non-stick substance system
At.
In the present invention, it is preferred that the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE) (PTFE)
10-20 parts, 15-30 parts of anodised aluminium, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
In the present invention, it is preferred that the non-stick substance contains following components in parts by weight: polytetrafluoroethylene (PTFE) (PTFE)
14-16 parts, 20-25 parts of anodised aluminium, 15-20 parts of ceramics, 10-15 parts of silica gel, 20-30 parts of enamelled cast iron.
The non-stick substance of above-described embodiment 1 to embodiment 4 is produced into sheet cell culture medium according to above-mentioned technique respectively,
The four kinds of sheet cell culture mediums obtained under the conditions of four kinds of embodiments are denoted as respectively: 1 sample of embodiment, is implemented 2 sample of embodiment
3 sample of example, 4 sample of embodiment, by obtained 1 sample of embodiment, 2 sample of embodiment, 3 sample of embodiment, 4 sample of embodiment and
1 sample of control group carries out following tests.
Test one:
1) 1 sample of Example, 2 sample of embodiment, 3 sample of embodiment, 4 sample of embodiment and 1 sample of control group each 50
Kilogram, pour five Agitation Tanks into respectively, in 1 minute, difference test room numbers of particles；Record is as shown in table 1 below:
Test result analysis:
As seen from the experiment, 1 sample of embodiment to 4 sample of embodiment sheet cell culture medium numbers of particles it is average
For 103.75/cubic metre, and the numbers of particles of the cell culture medium of 1 sample of control group is 1001/cubic metre, embodiment 1
The particle of sheet cell culture medium of sample to 4 sample of embodiment is about 1mm-25cm, therefore, user weigh, transport,
And dust is not generated during preparing, the influence to the health of user is smaller, and lowers user's production or experiment
When the cross contamination that generates.It can be seen that being used for by the formed punch position at the formed punch position and undershoot of the upper punching in tablet press machine
The material of the contact layer contacted with tabletting using the non-stick substance in the present invention formula, can reduce weigh, transport with
And the problem that dust in preparation process is high.
Test two:
1 sample of Example, 2 sample of embodiment, 3 sample of embodiment, 4 sample of embodiment and each 5 grams of 1 sample of control group,
It pours into 250 milliliters of waters for injection, is stirred with magnetic bar respectively, record each sample is completely dissolved the time, records such as the following table 2 institute
Show:
Test result analysis:
As seen from the experiment, 1 sample of embodiment to 4 sample of embodiment sheet cell culture medium dissolution time it is average
It is 93.75 seconds, and the dissolution time of the cell culture medium of 1 sample of control group is 280 seconds, it can be seen that, sheet cell culture medium
It during dissolution, can sink in liquid, reduce dissolution time.
It can be seen that by the formed punch position at the formed punch position and undershoot of the upper punching in tablet press machine for being contacted with tabletting
Contact layer material using the present invention in non-stick substance formula, it is possible to reduce the dissolution time of cell culture medium, solution
The certainly long problem of dissolution time.
Test three:
3) 1 sample of Example, 2 sample of embodiment, 3 sample of embodiment, 4 sample of embodiment and 1 sample of control group are each
Sample is poured into 50L water for injection respectively, and is stirred with magnetic bar by 1kg, is completely dissolved and is configured to 50L liquid, and record training is each
Sample is completely dissolved the time, and record is as shown in table 3 below:
Test result analysis:
As seen from the experiment, 1 sample of embodiment to 4 sample of embodiment sheet cell culture medium dissolution time it is average
Be 25.5 seconds, and the dissolution time of the cell culture medium of 1 sample of control group be 45 seconds, sheet cell culture medium using when be not required to claim
Amount is quantitative, and the required preparation time is short, therefore, passes through being used for for the formed punch position at the formed punch position and undershoot of the upper punching in tablet press machine
The material of the contact layer contacted with tabletting can be lowered weighing and be easy in the process using the formula of the non-stick substance in the present invention
The risk of mistake weighing and cross contamination occurs.
1 sample of control group:
Control group 1 is the punch of the undershoot of the contact layer for contacting with tabletting at the formed punch position of upper punching, tablet press machine
Sheet cell culture medium made when non-stick substance is not used in the contact layer for contacting with tabletting of position.I.e. using existing
There is sheet cell culture medium made of the conventional tabletting means in technology.
Claims (10)
1. a kind of tablet forming technique of sheet cell culture medium, it is characterised in that: include the following steps:
(S41), a tablet press machine, the material right and wrong for the contact layer that the formed punch position of the upper punching of tablet press machine is used to contact with tabletting are selected
The material of stickum, the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-stick substance；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, will be in middle die hole
Cell culture medium dry powder be pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
2. the tablet forming technique of sheet cell culture medium according to claim 1, it is characterised in that: the punch of the upper punching
The contact layer of position is arranged by way of package, and the contact layer at the formed punch position of the undershoot is set by way of package
It sets.
3. the tablet forming technique of sheet cell culture medium according to claim 1, it is characterised in that: the upper punching is by non-stick
Made of property substance, the undershoot is made of non-stick substance.
4. the tablet forming technique of sheet cell culture medium described in any one claim in -3 according to claim 1, special
Sign is: the non-stick substance contains following components in parts by weight: 10-20 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium
15-30 parts, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
5. the tablet forming technique of sheet cell culture medium according to claim 4, it is characterised in that: the non-stick substance contains
There is following components in parts by weight: 14-16 parts of polytetrafluoroethylene (PTFE) (PTFE), 20-25 parts of anodised aluminium, ceramics 15-20 parts, silica gel
10-15 parts, 20-30 parts of enamelled cast iron.
6. a kind of production technology of sheet cell culture medium, it is characterised in that:
(S1), it is pre-mixed；It is 20-40 parts sugared by 0-30 parts of amino acid 1,30-70 parts of salt, 10-20 parts of buffer mixing, in temperature
It is 5-40 DEG C, under conditions of humidity is less than 50%, powder is layered by diffusion blender device by diffusion movement, under external force
Isolated powder is moved in newborn level, so that each component powder is spread realization in subrange and is uniformly distributed, after 0.5-10h
Form premix；
(S2), it grinds；Above-mentioned premix is put into beveller, maintains the temperature at 4-80 DEG C, spin finishing instrument is to premixing
Object is ground, and the granular size that end product is made is 50-150 μm；
(S3), rear mixing；By smashed culture medium dry powder at 5-40 DEG C, humidity be less than 50% under conditions of, pass through diffusion
Powder is layered diffusion movement by hybrid machine, and the powder separated under external force is moved in newborn level, makes each component powder
Body spreads realization in subrange and is uniformly distributed, and needs 0.5-10h；
(S4), tabletting:
(S41), a tablet press machine is selected, the material of the contact layer for contacting with tabletting at the formed punch position of the upper punching of tablet press machine is
The material of non-stick substance, the contact layer for contacting with tabletting at the formed punch position of the undershoot of tablet press machine is non-stick substance；
(S42), middle die hole is protruded by middle die hole lower end in the formed punch position of undershoot, seals middle die hole bottom；
(S43), using feeder into middle die hole tytosis culture medium dry powder；
(S44), the formed punch position of upper punching is fallen into middle die hole from middle die hole upper end, and the scheduled stroke of downlink, will be in middle die hole
Cell culture medium dry powder be pressed into cell culture substrate；
(S45), emersion hole is purged on, is rushed in rotation while portalling, and the angle of punch rotating is greater than 30 degree；
(S46), undershoot rises die hole in the ejection of cell culture substrate, completes a tableting processes with this；
(S47), undershoot drops to original position, repeats step (S41) to (S46), and completion is filled next time.
7. the production technology of sheet cell culture medium according to claim 5, it is characterised in that: the punch of the upper punching
The contact layer of position is arranged by way of package, and the contact layer at the formed punch position of the undershoot is set by way of package
It sets.
8. the tablet forming technique of sheet cell culture medium according to claim 5, it is characterised in that: the upper punching is by non-stick
Made of property substance, the undershoot is made of non-stick substance.
9. special according to the production technology of sheet cell culture medium described in any one claim in claim 5-7
Sign is: the non-stick substance contains following components in parts by weight: 10-20 parts of polytetrafluoroethylene (PTFE) (PTFE), anodised aluminium
15-30 parts, 5-30 parts of ceramics, 5-20 parts of silica gel, 10-40 parts of enamelled cast iron.
10. the production technology of sheet cell culture medium according to claim 9, it is characterised in that: the non-stick substance
Contain following components in parts by weight: 14-16 parts of polytetrafluoroethylene (PTFE) (PTFE), 20-25 parts of anodised aluminium, ceramics 15-20 parts, silicon
10-15 parts of glue, 20-30 parts of enamelled cast iron.
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| CN201810875072.9A CN109130293A (en) | 2018-08-03 | 2018-08-03 | The tablet forming technique and its production technology of sheet cell culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114555776A (en) * | 2019-09-19 | 2022-05-27 | 生命技术公司 | Cell culture medium tablet and manufacturing method |
| CN115812826A (en) * | 2022-12-29 | 2023-03-21 | 烟台东宇海珍品有限公司 | Sea cucumber flower tablet candy and preparation process thereof |
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| CN106619549A (en) * | 2017-01-03 | 2017-05-10 | 江苏吴中医药集团有限公司苏州制药厂 | Ticagrelor and aspirin compound tablet and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114555776A (en) * | 2019-09-19 | 2022-05-27 | 生命技术公司 | Cell culture medium tablet and manufacturing method |
| CN115812826A (en) * | 2022-12-29 | 2023-03-21 | 烟台东宇海珍品有限公司 | Sea cucumber flower tablet candy and preparation process thereof |
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