CN109116034B - Homocysteine detection kit with strong anti-interference performance - Google Patents

Homocysteine detection kit with strong anti-interference performance Download PDF

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Publication number
CN109116034B
CN109116034B CN201811002543.1A CN201811002543A CN109116034B CN 109116034 B CN109116034 B CN 109116034B CN 201811002543 A CN201811002543 A CN 201811002543A CN 109116034 B CN109116034 B CN 109116034B
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CN
China
Prior art keywords
reagent bottle
box body
transverse plate
placing space
plate
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CN201811002543.1A
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CN109116034A (en
Inventor
曾宪亮
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Nanjing Aubrime Abm Biotechnology Co ltd
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Nanjing Aubrime Abm Biotechnology Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806Determination of free amino acids
    • G01N33/6812Assays for specific amino acids
    • G01N33/6815Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine

Abstract

The invention discloses a homocysteine detection kit with strong anti-interference performance, which comprises a controller, a touch screen, an outer box body, an inner box body and a top cover, wherein a cavity is formed in the top cover, a lower transverse plate and an upper transverse plate are arranged in the outer box body, 4 reagent bottle placing spaces are arranged in the inner box body, each reagent bottle placing space is communicated with a buffer space through a first channel, each reagent bottle placing space is communicated with the cavity through a second channel, a circulation channel is formed in the second transverse plate, a first through hole for communicating the buffer space with the circulation channel is formed in the upper transverse plate, a second through hole communicated with the cavity is formed in the top cover, a third through hole for communicating the second through hole with the circulation channel is formed in a sealing gasket, a second fan is arranged in the circulation channel, and a third valve is arranged in each first channel. The kit of the present invention can provide limited control of the temperature of the reagents stored therein and provide buffering to the reagent bottles without deterioration or damage.

Description

Homocysteine detection kit with strong anti-interference performance
Technical Field
The invention relates to the field of biochemistry, and particularly relates to a homocysteine detection kit with strong anti-interference performance.
Background
Homocysteine is a sulfur-containing amino acid that is produced intracellularly by demethylation of methionine. Recent research shows that homocysteine damages vascular endothelial cells by generating superoxide and peroxide, changes the function of blood coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the morbidity and mortality of cardiovascular diseases, so that the determination of the concentration of homocysteine in blood is clinically significant. There are many methods for measuring homocysteine in plasma, including High Performance Liquid Chromatography (HPLC), a cycloenzyme method, Fluorescence Polarization Immunoassay (FPIA), enzyme-linked immunoassay (EIA), amino acid analyzer measurement, ion chromatography, capillary gas chromatography-mass spectrometry, electrochemical methods, and the like. In the transportation and use process of the reagent, the reagent is liquid, so that the reagent is difficult to transport, store and the like, and is easy to break, flow out and lose efficacy, the homocysteine determination reagent needs to be kept at the temperature of 2-8 ℃, and the homocysteine determination reagent is safe and stable, and the existing kit cannot meet the requirements of the reagent.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to overcome the defects of the prior art and provides a homocysteine detection kit with strong anti-interference performance.
The technical scheme is as follows: a kit comprises a controller, a touch screen, an outer box body, an inner box body and a top cover, wherein the inner box body is fixed in the outer box body, the top cover is used for sealing the top opening of the outer box body, a sealing gasket is arranged at the position of the top cover, a cavity is arranged in the top cover, a lower transverse plate and an upper transverse plate are arranged in the outer box body, the upper transverse plate is positioned between the inner box body and the lower transverse plate, the inner box body and the outer box body are both cuboid, a bottom cover is arranged at the bottom of the outer box body, an ice bag placing space is formed between the bottom of the outer box body and the lower transverse plate, a lower connecting through hole is arranged at the position of the lower transverse plate, a first valve is arranged at the position of the lower connecting through hole, an upper connecting through hole is arranged at the position of the upper transverse plate, a second valve is arranged at the position of the upper connecting through hole, a heating device and a first temperature, the inner box body is internally provided with a first partition plate, a second partition plate and a third partition plate which divide the space in the inner box body into 4 reagent bottle placing spaces, each reagent bottle placing space is used for placing a reagent bottle, each reagent bottle placing space is communicated with the buffer space through a first channel, each reagent bottle placing space is communicated with the cavity through a second channel, the second partition plate is internally provided with a circulation channel, the bottom of the inner box body is provided with a first through hole communicated with the buffer space and the circulation channel, the top cover is provided with a second through hole communicated with the cavity, the sealing pad is provided with a third through hole communicated with the second through hole and the circulation channel, and the circulation channel is internally provided with a second fan; each first channel is internally provided with a third valve, each reagent bottle placing space is internally provided with an upper frame part, a lower frame part, a light emitter, a light receiver and a second temperature sensor, each reagent bottle placing space is also internally provided with a hemispherical reagent bottle support, each reagent bottle support is provided with a groove for placing a reagent bottle, the reagent bottle support is fixed with the bottom of the inner box body through a first spring, the top cover is also connected with a pressing plate through a second spring, when the top cover covers the outer box body, each reagent bottle placing space is internally provided with a pressing plate, and each pressing plate is provided with 2 pressing blocks which are used for abutting and extruding the reagent bottle; when a reagent bottle is placed in a certain reagent bottle placing space, the optical receiver cannot receive an optical signal of the optical transmitter, the pressing plate is not abutted with the upper frame part, and the reagent bottle support is not abutted with the lower frame part; when no reagent bottle is placed in a certain reagent bottle placing space, the pressing plate is abutted to the upper frame portion, the reagent bottle support is abutted to the lower frame portion, and the light receiver can receive light rays of the light emitter.
Furthermore, two lower connecting through holes are formed in the lower transverse plate, and 5 upper connecting through holes are formed in the upper transverse plate; the upper transverse plate is provided with 2 heating devices.
Further, the inner case has 4 side plates, the outer case has 4 side plates, and an outer side of one side plate of each inner case is fixed to an inner side of one side plate of the outer case.
Further, the bottom cover is hinged with the bottom of the outer box body; the first fan is fixed with the lower transverse plate through the first support and is fixed with the upper transverse plate through the second support.
Further, a heat insulation layer is arranged between the side plate of the outer box body and the inner side and the outer side of the bottom, a heat insulation layer is arranged between the side plate of the inner box body and the inner side and the outer side of the bottom, and heat insulation layers are arranged in the first partition plate, the second partition plate and the third partition plate.
Further, the upper surface of the upper frame portion and the lower surface of the lower frame portion are both provided with rubber pads.
Further, each reagent bottle placing space has 4 side walls, and each reagent bottle placing space has 4 third springs, and each side wall is connected with the reagent bottle holder through one third spring.
Further, when the reagent kit is in an open state, the controller controls to display the measurement value of the first temperature sensor and the measurement value of the second temperature sensor in each reagent bottle placing space on the touch screen.
Further, when the reagent box is in an opening state, the controller controls to open the first fan and the heating device and close the first valve when the measurement value of the first temperature sensor is smaller than the lower limit value; when the measured value of the first temperature sensor is greater than the upper limit value, the first fan and the first valve are opened, and the heating device is closed; when the reagent box is in an opening state, the controller controls, in the reagent bottle placing space where the optical receiver cannot receive the optical signal of the optical emitter, when the measured value of the second temperature sensor in more than one reagent bottle placing space is not in the set range, the temperature adjusting operation is started, in the temperature adjusting operation, the second fan and the second valve are opened, and the third valve of the first channel corresponding to the reagent bottle placing space where the measured value of the second temperature sensor is not in the set range is opened.
Further, the lower limit value is larger than the minimum value of the set range, and the upper limit value is smaller than the maximum value of the set range.
Further, one of the upper connecting through holes is located below the first through hole, and the remaining four upper connecting through holes are each located below one of the first channels.
Further, when the temperature adjustment operation is continuously triggered for 3 times because the same reagent bottle is placed in the space, the controller displays an alarm signal on the touch screen and displays the serial number of the reagent bottle placing space.
Theoretically, under the condition that the reagent amount of the reagent bottles is almost the same, the temperature in each reagent bottle placing space is basically the same, the reagent bottle placing space triggering the temperature adjusting operation each time is random, if the temperature adjusting operation is always triggered by the same reagent bottle placing space, the problem possibly occurs in the reagent bottle placing space, and the alarm is given in time to remind maintenance. Of course, the operator may also determine that if the number of reagent bottles in the reagent bottle holding space is greatly different from that of the other reagent bottles, or if only one reagent bottle is held in the reagent bottle holding space, the operator may cancel the alarm in case of false alarms. When there is no such false alarm, the operator can judge that there may be a problem with the reagent bottle placing space. Such alarms are not completely accurate and there is a possibility of false alarms, but also provide a way to detect faults.
Further, in the temperature adjustment operation, the second fan and the second valve are opened, the third valve of the first passage corresponding to the reagent bottle placing space whose measurement value of the second temperature sensor is not within the set range is opened, and the third valves of all the remaining first passages are closed.
Further, the lower limit value is 4 ℃ and the upper limit value is 6 ℃.
Further, the set range is 3 ℃ or more and 7 ℃ or less.
Has the advantages that: the kit can control the environmental temperature of the reagent placed in the kit in a limited way, so that the reagent is protected from deterioration, and the reagent support and the pressing plate can buffer the reagent and are not easy to damage in the transportation process. The kit of the invention is particularly suitable for short-distance transportation determination reagents.
Drawings
FIG. 1 is a schematic diagram of a kit.
Detailed Description
1 an outer box body; 1.1 bottom cover; 1.2, an ice bag; 1.3, a lower transverse plate; 1.4 first valve; 1.5 a first fan; 1.6, an upper transverse plate; 1.7 a second valve; 1.8 heating device; 1.9 a first temperature sensor; 2, an inner box body; 2.1 a first separator; 2.2 a second separator; 2.3 a third separator; 2.2.1 flow-through channels; 2.2.2 second fan; 3, top cover; 3.1 sealing gasket; 3.2 a cavity; 3.3 a second channel; 4.1 a first spring; 4.2 pressing plates; 4.3, briquetting; 4.4 upper frame portion; 4.5 lower frame part; 4.6 reagent bottle holder; 4.7 third spring; 4.8 second spring; 4.9 third valve; 4.10 a second temperature sensor; 4.11 light emitters; 4.12 an optical receiver; 5 a buffer space; 5.1 a first via; 10 reagent bottle.
The utility model provides a kit, includes controller, touch-sensitive screen, outer box 1, interior box 2 and top cap 3, interior box 2 is fixed in outer box 1, top cap 3 is used for sealing the open-top of outer box, and top cap department has sealed pad 3.1, cavity 3.2 has in the top cap, have down diaphragm 1.3 and last diaphragm 1.6 in the outer box, it is located between box 2 and the diaphragm 1.3 down to go up diaphragm 1.6, interior box and outer box are the cuboid form, the bottom of outer box has bottom 1.1, form ice bag between the bottom of outer box and the diaphragm down and place the space, diaphragm department has down through-hole, and lower through-hole department has first valve 1.4, go up diaphragm department and have connecting hole, go up connecting hole department and have second valve 1.7, go up diaphragm department and still install heating device 1.8 and first temperature sensor 1.6, go up, A first fan 1.5 is arranged between the lower transverse plates, a buffer space 5 is formed between the upper transverse plates and the bottom of the inner box body, the inner box body is internally provided with a first clapboard 2.1, a second clapboard 2.2 and a third clapboard 2.3 which divide the space in the inner box body into 4 reagent bottle placing spaces, each reagent bottle placing space is used for placing a reagent bottle 10, each reagent bottle placing space is communicated with the buffer space through a first channel, and each reagent bottle placing space is communicated with the cavity 3.2 through a second channel 3.3, the second clapboard is internally provided with a flow passage 2.2.1, the bottom of the inner box body is provided with a first through hole for communicating the buffer space with the flow passage, the top cover is provided with a second through hole communicated with the cavity, the sealing gasket is provided with a third through hole communicated with the second through hole and a flow channel, and a second fan is arranged in the flow channel; each first channel is internally provided with a third valve 4.9, each reagent bottle placing space is internally provided with an upper frame portion 4.4, a lower frame portion 4.5, a light emitter 4.11, a light receiver 4.12 and a second temperature sensor 4.10, each reagent bottle placing space is also internally provided with a hemispherical reagent bottle holder 4.6, each reagent bottle holder is provided with a groove for placing a reagent bottle, the reagent bottle holder is fixed with the bottom of the inner box body through a first spring 4.8, the top cover is also connected with a pressing plate 4.2 through a second spring 4.1, when the top cover covers the outer box body, each reagent bottle placing space is internally provided with a pressing plate, and each pressing plate is provided with 2 pressing blocks which are used for abutting against and pressing the reagent bottle 10; when a reagent bottle is placed in a certain reagent bottle placing space, the optical receiver cannot receive an optical signal of the optical transmitter, the pressing plate is not abutted with the upper frame part, and the reagent bottle support 4.6 is not abutted with the lower frame part 4.5; when no reagent bottle is placed in a certain reagent bottle placing space, the pressing plate 4.2 is abutted against the upper frame portion 4.4, the reagent bottle support is abutted against the lower frame portion, and the light receiver can receive the light of the light emitter.
The lower transverse plate is provided with two lower connecting through holes, and the upper transverse plate is provided with 5 upper connecting through holes; the upper transverse plate is provided with 2 heating devices. The inner box body is provided with 4 side plates, the outer box body is provided with 4 side plates, and the outer side of one side plate of each inner box body is fixed with the inner side of one side plate of the outer box body. The bottom cover is hinged with the bottom of the outer box body; the first fan is fixed with the lower transverse plate through the first support and is fixed with the upper transverse plate through the second support. The heat insulation layer is arranged between the side plate of the outer box body and the inner side and the outer side of the bottom, the heat insulation layer is arranged between the side plate of the inner box body and the inner side and the outer side of the bottom, and the heat insulation layers are arranged in the first partition plate, the second partition plate and the third partition plate. The upper surface of the upper frame portion and the lower surface of the lower frame portion are both provided with rubber pads. Each reagent bottle placing space is provided with 4 side walls, each reagent bottle placing space is provided with 4 third springs, and each side wall is connected with the reagent bottle support through one third spring. When the reagent box is in an opening state, the controller controls to display the measurement value of the first temperature sensor and the measurement value of the second temperature sensor in each reagent bottle placing space on the touch screen.
As shown in the figure, each reagent bottle placing space can be used for placing one reagent bottle, the reagent bottle is clamped between the pressing plate and the reagent bottle holder, the reagent bottle is spread by the pressing plate and the reagent bottle holder, the reagent bottle holding space can be communicated with the cavity and the buffer space, and the third valve is required to be opened when the reagent bottle holding space is communicated with the buffer space. When not placing the reagent bottle in the space is placed to the reagent bottle, the rubber pad butt of clamp plate and upper frame portion, the rubber pad of lower frame portion and the upper surface butt that the reagent bottle dragged to this reagent bottle place the space by independent, all not communicate with cavity and buffer space.
When the reagent box is in an opening state, the controller controls, and when the measurement value of the first temperature sensor is smaller than the lower limit value, the first fan and the heating device are opened, and the first valve is closed; when the measured value of the first temperature sensor is greater than the upper limit value, the first fan and the first valve are opened, and the heating device is closed;
by such control, the temperature of the air between the upper and lower cross plates is always maintained between the upper and lower limit values. The first fan can keep the space temperature between the upper transverse plate and the lower transverse plate to be rapid and uniform.
When the reagent box is in an opening state, the controller controls, in the reagent bottle placing space where the optical receiver cannot receive the optical signal of the optical emitter, when the measured value of the second temperature sensor in more than one reagent bottle placing space is not in the set range, the temperature adjusting operation is started, in the temperature adjusting operation, the second fan and the second valve are opened, and the third valve of the first channel corresponding to the reagent bottle placing space where the measured value of the second temperature sensor is not in the set range is opened. The lower limit value is larger than the minimum value of the set range, and the upper limit value is smaller than the maximum value of the set range.
Through such control, when the temperature in certain reagent bottle placing space in which the reagent bottle is placed is not in the set range, the air in the reagent bottle placing space and the air between the upper transverse plate and the lower transverse plate generate heat exchange, so that the temperature in the reagent bottle placing space is restored to the set range, and the reagent is ensured not to deteriorate.
While the invention has been shown and described with respect to the preferred embodiments, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the invention as defined in the following claims.

Claims (10)

1. The homocysteine detection kit with strong interference resistance is characterized by comprising a controller, a touch screen, an outer box body, an inner box body and a top cover, wherein the inner box body is fixed in the outer box body, the top cover is used for sealing the top opening of the outer box body, a sealing gasket is arranged at the position of the top cover, a cavity is arranged in the top cover, a lower transverse plate and an upper transverse plate are arranged in the outer box body, the upper transverse plate is positioned between the inner box body and the lower transverse plate, the inner box body and the outer box body are both cuboid, a bottom cover is arranged at the bottom of the outer box body, an ice bag placing space is formed between the bottom of the outer box body and the lower transverse plate, a lower connecting through hole is arranged at the lower transverse plate, a first valve is arranged at the position of the lower connecting through hole, an upper connecting through hole is arranged at the position of the upper transverse plate, a second valve is, a first fan is arranged between the upper transverse plate and the lower transverse plate, a buffer space is formed between the upper transverse plate and the bottom of the inner box body, a first partition plate, a second partition plate and a third partition plate are arranged in the inner box body to divide the space in the inner box body into 4 reagent bottle placing spaces, each reagent bottle placing space is used for placing a reagent bottle, each reagent bottle placing space is communicated with the buffer space through a first channel, each reagent bottle placing space is communicated with the cavity through a second channel, a circulation channel is arranged in the second partition plate, a first through hole communicated with the buffer space and the circulation channel is arranged at the bottom of the inner box body, a second through hole communicated with the cavity is arranged at the top cover, a third through hole communicated with the second through hole and the circulation channel is arranged at the sealing gasket, and a second fan is arranged in the circulation channel; each first channel is internally provided with a third valve, each reagent bottle placing space is internally provided with an upper frame part, a lower frame part, a light emitter, a light receiver and a second temperature sensor, each reagent bottle placing space is also internally provided with a hemispherical reagent bottle support, each reagent bottle support is provided with a groove for placing a reagent bottle, the reagent bottle support is fixed with the bottom of the inner box body through a first spring, the top cover is also connected with a pressing plate through a second spring, when the top cover covers the outer box body, each reagent bottle placing space is internally provided with a pressing plate, and each pressing plate is provided with 2 pressing blocks which are used for abutting and extruding the reagent bottle; when a reagent bottle is placed in a certain reagent bottle placing space, the optical receiver cannot receive an optical signal of the optical transmitter, the pressing plate is not abutted with the upper frame part, and the reagent bottle support is not abutted with the lower frame part; when no reagent bottle is placed in a certain reagent bottle placing space, the pressing plate is abutted to the upper frame portion, the reagent bottle support is abutted to the lower frame portion, and the light receiver can receive light rays of the light emitter.
2. The homocysteine detection kit with strong interference resistance according to claim 1, wherein the lower horizontal plate is provided with two lower connecting through holes, and the upper horizontal plate is provided with 5 upper connecting through holes; the upper transverse plate is provided with 2 heating devices.
3. The homocysteine detection kit with high interference resistance according to claim 1 wherein the inner box has 4 side plates and the outer box has 4 side plates, and the outer side of one side plate of each inner box is fixed with the inner side of one side plate of the outer box.
4. The homocysteine detection kit with strong interference resistance according to claim 1, wherein the bottom cover is hinged with the bottom of the outer box body; the first fan is fixed with the lower transverse plate through the first support and is fixed with the upper transverse plate through the second support.
5. The homocysteine detection kit with high interference resistance according to claim 1, wherein a heat insulation layer is arranged between the side plate of the outer box body and the inner side and the outer side of the bottom, a heat insulation layer is arranged between the side plate of the inner box body and the inner side and the outer side of the bottom, and heat insulation layers are arranged in the first, second and third partition plates.
6. The highly interference-resistant homocysteine detection kit according to claim 1 wherein the upper surface of the upper frame portion and the lower surface of the lower frame portion are both provided with rubber pads.
7. The homocysteine detection kit with strong interference resistance according to claim 1 wherein each reagent bottle placing space has 4 side walls and each reagent bottle placing space has 4 third springs, and each side wall is connected with the reagent bottle holder by one third spring.
8. The homocysteine detection kit with high interference resistance according to claim 1, wherein when the kit is in an open state, the controller controls to display the measurement value of the first temperature sensor and the measurement value of the second temperature sensor in each reagent bottle placing space on the touch screen.
9. The homocysteine detection kit with strong interference resistance according to claim 1, wherein when the kit is in an open state, the controller controls to open the first fan and the heating device and close the first valve when the measurement value of the first temperature sensor is less than the lower limit value; when the measured value of the first temperature sensor is greater than the upper limit value, the first fan and the first valve are opened, and the heating device is closed; when the reagent box is in an opening state, the controller controls, in the reagent bottle placing space where the light receiver cannot receive the light signal of the light emitter, when the measured value of the second temperature sensor in more than one reagent bottle placing space is not in the set range, the temperature adjusting operation is started, in the temperature adjusting operation, the second fan and the second valve are opened, and the third valve of the first channel corresponding to the reagent bottle placing space where the measured value of the second temperature sensor is not in the set range is opened.
10. The homocysteine detection kit with strong interference resistance according to claim 9, wherein the lower limit value is larger than the minimum value of the set range, and the upper limit value is smaller than the maximum value of the set range.
CN201811002543.1A 2018-08-30 2018-08-30 Homocysteine detection kit with strong anti-interference performance Active CN109116034B (en)

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CN201811002543.1A CN109116034B (en) 2018-08-30 2018-08-30 Homocysteine detection kit with strong anti-interference performance

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Application Number Priority Date Filing Date Title
CN201811002543.1A CN109116034B (en) 2018-08-30 2018-08-30 Homocysteine detection kit with strong anti-interference performance

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CN109116034B true CN109116034B (en) 2021-06-22

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