CN109115685A - Electronic Speculum ultra-thin section aided positioning system - Google Patents
Electronic Speculum ultra-thin section aided positioning system Download PDFInfo
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- CN109115685A CN109115685A CN201811275994.2A CN201811275994A CN109115685A CN 109115685 A CN109115685 A CN 109115685A CN 201811275994 A CN201811275994 A CN 201811275994A CN 109115685 A CN109115685 A CN 109115685A
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- translation stage
- thin section
- lifting platform
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses a kind of Electronic Speculum ultra-thin section aided positioning systems, including mechanical support module, optical imagery module and image processing module, optical imagery module is arranged in mechanical support module, image processing module is connected with optical imagery module, optical imagery module includes continuous zoom microscope tube, object lens and auto-focusing CCD, it is characterized in that, mechanical support module includes shockproof bottom plate, lifting platform, the first translation stage and the second translation stage；Lifting platform is arranged on shockproof bottom plate and can vertically move on shockproof bottom plate；First translation stage and the second translation stage arranged in a crossed manner can move in parallel on lifting platform and on lifting platform in 90 degree.The invention can real-time in-situ observe ultrathin section sample, lateral incision face, accurately measure Sample location deviation angle, without frequently removing specimen holder observation from slicer, solves jitter problem when holding specimen holder observation section in conventional method, promote practical teaching work.
Description
Technical field
The present invention relates to ultramicrotome field more particularly to a kind of Electronic Speculum ultra-thin section aided positioning systems.
Background technique
Ultra-thin section is one of the important technology of electron microscopic sample preparation.It, need to be to the spy of sample with the development of scientific research
Determine position carry out accurate ultra-thin section field it is also more and more, as butterfly wing, insect crust elytrum, animal tissue's composite material and
Plant roots and stems etc. are related to multiple scientific researches such as bionics, medicine, materialogy and botany or industry direction.Ultra-thin section positions skill
Art is the particular demands according to sample, and the privileged site for choosing sample is precisely sliced, and location technology is also ultra-thin section skill
Difficult point in art.Traditional ultra-thin section location technology, the sample that need to usually lean on naked eyes to be observed in embedded block under the microscope are cut
Specimen holder after the deflection angle for then estimating setting specimen holder, with repairing block knife serial section, then is removed, hand in face from sample arm
It holds sample and checks whether section reaches positioning requirements under object lens.The above process need to be repeated multiple times, can complete orientation, non-
Normal cumbersome time-consuming.Early-stage study in terms of ultra-thin section location technology is considerably less.Currently, there is no the ultra-thin section for being related to hardware fixed
The research of position technical aspect.Existing ultra-thin section Position Research also only for specific cell or tissue sample, and fails to solve
The certainly cumbersome problem of orientation operating process.Currently, all ultramicrotome, own hardware are unable to satisfy efficiently on the market
Easily carry out the demand of ultra-thin section positioning.
The problem to be solved in the present invention is how real-time in-situ observes ultrathin section sample, lateral incision face, and it is fixed to accurately measure sample
Position deviation angle solves to hold jitter problem when specimen holder observation section in conventional method.
Therefore, those skilled in the art is dedicated to developing a kind of Electronic Speculum ultra-thin section aided positioning system, can be former in real time
Position observation ultra-thin section sample lateral incision face, accurately measures the positioning runout angle of sample, without removing specimen holder from slicer
Observation, streamline operation greatly improve location efficiency, promote the practical teaching of ultramicrotomy.
Summary of the invention
In view of the drawbacks described above of the prior art, the technical problem to be solved by the present invention is to how real-time in-situ observation is ultra-thin
Section sample lateral incision face accurately measures Sample location deviation angle, in solution conventional method when hand-held specimen holder observation section
Jitter problem.
To achieve the above object, the present invention provides a kind of Electronic Speculum ultra-thin section aided positioning systems, including mechanical support
Module, optical imagery module and image processing module, optical imagery module are arranged in mechanical support module, image processing module
It is connected with optical imagery module, optical imagery module includes continuous zoom microscope tube, object lens and auto-focusing CCD, feature
It is, mechanical support module includes shockproof bottom plate, lifting platform, the first translation stage and the second translation stage；Lifting platform is arranged shockproof
It can vertically move on bottom plate and on shockproof bottom plate；First translation stage and the second translation stage are arranged in a crossed manner on lifting platform in 90 degree
And it can be moved in parallel on lifting platform.
Further, shockproof bottom plate includes optical flat, and sets up the first in command in the both sides of the edge of optical flat separately
And second handle；Optical flat is square, and side length is 300~800mm, preferably 400mm.
Further, lifting platform is fixed on shockproof bottom plate.
Further, lifting platform carries out lift adjustment by lift adjustment knob and lock screw；The vertical shifting of lifting platform
Dynamic stroke range is 0~200mm, preferably 0~120mm.
Further, the first translation stage and the second translation stage are fixed on lifting platform by counterbore and screw.
Further, the first displacement regulation handle is provided on the first translation stage；Second is provided on second translation stage
Move regulation handle；The stroke range that moves in parallel of first translation stage and the second translation stage is 0~100mm, preferably 0~
50mm。
Further, object lens and auto-focusing CCD set up separately in the both ends of continuous zoom microscope tube, object lens and continuous zoom
Microscope tube is in horizontal positioned.
Further, the zoom range of continuous zoom microscope tube is 0.5~10 times, preferably 0.75~5 times；Object lens
Zoom range is 0.5~10 times, preferably 0.5~2 times；Optical imagery module by circular retainer ring, rectangle fixed block with
Right angle fixed block level is fixed on above mechanical support module.
Further, optical imagery module further includes universal optical fiber, sample to be tested be between universal optical fiber and object lens or
Side.
Further, image processing module includes host and display screen, is equipped with image processing software on host, optics at
Image processing module can be transmitted to as module acquired image and is shown on a display screen, image processing software measurement folder
Angle function can measure the practical deviation angle of sample section and Sample location face.Image processing software is ImageView.
The invention can real-time in-situ observe ultra-thin section sample lateral incision face, accurately measure the positioning runout angle of sample, nothing
Specimen holder observation need to be removed from slicer, streamline operation greatly improves location efficiency, promotes the reality of ultramicrotomy
Trample teaching.
It is described further below with reference to technical effect of the attached drawing to design of the invention, specific structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Detailed description of the invention
Fig. 1 is the Electronic Speculum ultra-thin section aided positioning system structural schematic diagram of a preferred embodiment of the invention；
Fig. 2 is affixed to the butterfly wing sample sterogram on specimen holder；
Fig. 3 is the real-time butterfly wing section acquisition figure of Electronic Speculum ultra-thin section aided positioning system；
Fig. 4 is that image processing software ImageView is applied to butterfly wing ultra-thin section positioning runout angle measurement effect picture；
Wherein, 1- optical flat, the 2- first in command, 3- lifting platform, 4- second displacement regulation handle, the first translation stage of 5-,
6- host, 7- display screen, the displacement regulation handle of 8- first, 9- data line, 10- second handle, 11- lift adjustment knob,
12- lock screw, the second translation stage of 13-, the right angle 14- fixed block, 15- rectangle fixed block, 16- object lens, 17- continuous zoom are aobvious
Micro mirror cylinder, 18- fixed screw, 19- fixed ring, 20- autozoom CCD.
Specific embodiment
Multiple preferred embodiments of the invention are introduced below with reference to Figure of description, keep its technology contents more clear and just
In understanding.The present invention can be emerged from by many various forms of embodiments, and protection scope of the present invention not only limits
The embodiment that Yu Wenzhong is mentioned.
In the accompanying drawings, the identical component of structure is indicated with same numbers label, everywhere the similar component of structure or function with
Like numeral label indicates.The size and thickness of each component shown in the drawings are to be arbitrarily shown, and there is no limit by the present invention
The size and thickness of each component.Apparent in order to make to illustrate, some places suitably exaggerate the thickness of component in attached drawing.
As shown in Figure 1, ultra-thin section aided positioning system, includes mechanical support module, optical imagery module and image altogether
Processing module three parts.
Mechanical support module mainly realizes shockproof support and all around (XY axis) translation and vertical direction (Z axis) lifting.
Micro-imaging equipment is for shockproof more demanding, to avoid environmental vibration from influencing the optical imaging effect of device, mechanical support
The shockproof bottom plate of module is made of the optical flat 1 with the first in command 2 and second handle 10, and optical flat 1 is square, side length
It is 400mm (being also possible to 300~800mm), weight about 5kg.The lifting platform 3 that fixation can be vertically moved up or down on optical flat 1 rises
Dropping stroke is 120mm (being also possible to 200mm), carries out lift adjustment by lift adjustment knob 11 and lock screw 12.By two
Block intersects high-precision first translation stage 5 placed and the second translation stage 13 in 90 ° to be consolidated by counterbore and four pieces of hexagon socket head cap screws
Due on lifting platform 3, the translating stroke of the first translation stage 5 and the second translation stage 13 is 50mm (being also possible to 100mm).First
Translation stage 5 is equipped with the first displacement regulation handle 8, and the second translation stage 13 is equipped with second displacement regulation handle 4.This design guarantees
It can be passed through according to the physical location of sample and adjust XYZ and axially carry out the accurate imaging focal length or height that adjust sample.
Optical imagery module mainly realizes real-time focusing and micro- amplification imaging function to ultrathin section sample, section.It should
Module is fixed on mechanical support module by circular retainer ring 19, rectangle fixed block 15 and 14 level of right angle fixed block customized
Top, fixed ring 19 are equipped with fixed screw 18.The main body of optical imagery module (is also possible to for 0.75-5 times by zoom range
0.5~10 times) continuous zoom microscope tube 17,0.5-2 times of zoom range (being also possible to 0.5~10 times) object lens 16 and tool
There are 2,000,000 pixels and the auto-focusing CCD 20 with cmos image sensor is constituted.Object lens 16 divide with auto-focusing CCD 20
Not Tong Guo screw thread abutting joint in the both ends of continuous zoom microscope tube 17, wherein object lens 16 are close to sample blocks lateral incision face.Due to
Sample blocks are translucent epoxy resin, therefore universal optical fiber can be used as light source in the side of specimen holder, can be according to sample
The position of product arbitrarily adjusts light source direction, provides illumination for sample blocks, to improve the light pass-through degree of sample blocks, helps to mention
The imaging effect of high acquired image.
The major function of image processing module is to carry out image processing and analyzing to the section of the ultra-thin section acquired in real time.It should
Module is connected by data line 9 with optical imagery module, and hardware is host 6 and display screen 7, and image processing software is
ImageView.By the included hotspot function of HDMI bidirectional data line or autozoom CCD 20, by autozoom CCD
The sample lateral incision face Image Real-time Transmission of 20 acquisitions is simultaneously projected on display screen 7.Using in image processing software ImageView
Measure angle function, can real―time precision measurment sample section and Sample location face practical deviation angle.
In actual use, the shockproof bottom plate equipped with ultra-thin section auxiliary locator is placed in the one of slicer sample arm
Side, object lens 16 adjust the height of object lens 16 to identical as sample blocks close to sample blocks.In the sample arm side of slicer, place
One double Zhi Wanxiang optical fiber is as light source.Autozoom CCD 20 is connect with host 6, is observed on display screen 7 collected
Image.Pass through the second displacement regulation handle 8 and the first displacement regulation handle 4 of the first translation stage 5 and the second translation stage 13 respectively
The distance for adjusting X-axis and Y-axis, makes image focus to clearest state.Image processing software ImageView is opened, " angle is chosen
Measurement " tool, that is, can accurately measure the deviation angle of section Yu Sample location face.
As shown in Fig. 2, being butterfly wing sample at arrow.Butterfly wing composite material is one of hot spot of bionics Study, and is needed
Use the conventional sample of ultra-thin section location technology.By taking butterfly wing composite material as an example, the practical application effect of the device is introduced.Butterfly
The scale on wing surface is in parallel striated texture, and researcher usually need to fine microstructures and pattern to its particular cross section
It is characterized, this is also the subsequent basis for carrying out related bionics Study.
As shown in Figure 3 and Figure 4, when making the ultra-thin section of butterfly wing composite material, the section of ultra-thin section need to generally be made
With the striped keeping parallelism of scale, to meet testing requirement.After the device, the reality of positioning section can be obtained to quicklook
When image, and the deviation angle of section can be accurately measured.Ultra-thin section auxiliary locator can be clear in real time in slicing processes
Ground reflects that situation is deviateed in the section of butterfly wing in embedded block, and avoiding needs frequently to remove the weight that sample blocks are observed in conventional method
Multiple labour, greatly improves slice efficiency.In an actual measurement example, which is 4.62 °, as shown in Figure 4.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (10)
1. a kind of Electronic Speculum ultra-thin section aided positioning system, including mechanical support module, optical imagery module and image procossing mould
Block, the optical imagery module are arranged in the mechanical support module, described image processing module and the optical imagery mould
Block is connected, and the optical imagery module includes continuous zoom microscope tube, object lens and auto-focusing CCD, which is characterized in that described
Mechanical support module includes shockproof bottom plate, lifting platform, the first translation stage and the second translation stage；The lifting platform setting is described anti-
It can vertically move on shake bottom plate and on the shockproof bottom plate；First translation stage and second translation stage are in 90 degree of intersections
It is arranged on the lifting platform and can be moved in parallel on the lifting platform.
2. Electronic Speculum ultra-thin section aided positioning system as described in claim 1, which is characterized in that the shockproof bottom plate includes light
Plate is learned, and sets up the first in command in the both sides of the edge of the optical flat and second handle separately；The optical flat is just
Rectangular, side length is 300~800mm.
3. Electronic Speculum ultra-thin section aided positioning system as described in claim 1, which is characterized in that the lifting platform fixed setting
On the shockproof bottom plate.
4. Electronic Speculum ultra-thin section aided positioning system as claimed in claim 3, which is characterized in that the lifting platform passes through lifting
Adjusting knob and lock screw carry out lift adjustment；The vertical moving stroke range of the lifting platform is 0~200mm.
5. Electronic Speculum ultra-thin section aided positioning system as described in claim 1, which is characterized in that first translation stage and institute
The second translation stage is stated to be fixed on the lifting platform by counterbore and screw.
6. Electronic Speculum ultra-thin section aided positioning system as claimed in claim 5, which is characterized in that set on first translation stage
It is equipped with the first displacement regulation handle；Second displacement regulation handle is provided on second translation stage；First translation stage and
The stroke range that moves in parallel of second translation stage is 0~100mm.
7. Electronic Speculum ultra-thin section aided positioning system as described in claim 1, which is characterized in that object lens and described automatic
Focusing CCD sets up separately in the both ends of the continuous zoom microscope tube, the object lens and the continuous zoom microscope tube in level
It places.
8. Electronic Speculum ultra-thin section aided positioning system as claimed in claim 7, which is characterized in that the continuous zoom microscope
The zoom range of cylinder is 0.5~10 times, and the zoom range of the object lens is 0.5~10 times；The optical imagery module passes through circle
Shape fixed ring, rectangle fixed block and right angle fixed block level are fixed on above the mechanical support module.
9. Electronic Speculum ultra-thin section aided positioning system as claimed in claim 1 or 7, which is characterized in that the optical imagery mould
Block further includes universal optical fiber, and sample to be tested is between the universal optical fiber and the object lens or side.
10. Electronic Speculum ultra-thin section aided positioning system as described in claim 1, which is characterized in that described image processing module
Including host and display screen, image processing software is installed, the optical imagery module acquired image can on the host
It is transmitted to described image processing module and is shown on the display screen, described image processing software measures angle function can
Measure the practical deviation angle of sample section and Sample location face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811275994.2A CN109115685B (en) | 2018-10-30 | 2018-10-30 | Auxiliary positioning system for ultrathin section of electron microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811275994.2A CN109115685B (en) | 2018-10-30 | 2018-10-30 | Auxiliary positioning system for ultrathin section of electron microscope |
Publications (2)
| Publication Number | Publication Date |
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| CN109115685A true CN109115685A (en) | 2019-01-01 |
| CN109115685B CN109115685B (en) | 2021-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811275994.2A Active CN109115685B (en) | 2018-10-30 | 2018-10-30 | Auxiliary positioning system for ultrathin section of electron microscope |
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| CN113484155A (en) * | 2021-07-06 | 2021-10-08 | 中国工程物理研究院激光聚变研究中心 | Ultrathin film mechanical property measuring device and method |
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|---|---|
| CN109115685B (en) | 2021-02-09 |
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