CN109112229A - A kind of shore wheat genome specific molecular marker primer and its application - Google Patents
A kind of shore wheat genome specific molecular marker primer and its application Download PDFInfo
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Abstract
The invention discloses a kind of shore wheat genome specific molecular marker primers, including 47 kinds of SSR label primers and 119 kinds of EST-STS labeled primers.Molecular labeling provided by the present invention can be used as the useful supplement of the prior art, pass through the method for polymerase chain reaction PCR, effectively distinguish the nearly edge species of common wheat and common wheat, derivative offspring especially containing shore wheat genome, it helps in molecular selection breeding process, fast and effeciently track the inhereditary material of shore wheat, it is also beneficial to the transfer by the excellent genes of shore wheat and merit to receptor Common Wheat Varieties simultaneously, breeding process is accelerated, the Breeding Efficiency and quality of new varieties are improved.
Description
Technical field
The invention belongs to technical field of biological genetic engineering, be related to a kind of shore wheat genome specific molecular marker primer and its
Using.
Background technique
Shore wheat (Leymus mollis (Trin) Hara.NsNsXmXm 2n=28) belongs to grass family (Poaceae), Tribe Triticeae
(Triticeae), barley subtribe (Hordinae), a perennial wheat of tetraploid of leymus (Leymus Hochst) are close
Edge kind wild plant, is mainly grown in seashore and inland arid area, is distributed in northern China coastal area, the Russian (Far East
Area), Korea, Japan and North America.It is spent more with stripe rust resisting, powdery mildew, head blight, aphid, drought resisting, saline-alkali tolerant, big fringe
Good characteristic.Last century the fifties, Soviet scientists start to carry out wheat and leymus includes the hybridization of shore wheat, save through embryo
Technology obtains the hybrid of tetraploid, hexaploid wheat and shore wheat, and the sixties obtain the amphidiploid of hybrid, the seventies
Obtain the incomplete amphidiploid of durum wheat Yu shore wheat.Fu Jie etc. creates a series of small with excellent economical character
Wheat-heterologous the germplasm line of shore wheat, these germplasm lines still have the characteristic of part shore wheat, especially to the resistance of stripe rust.Using remote
Multiple types excellent genes in the wheat of shore are imported into common wheat by the multiple means such as edge hybridization, chromosome engineering, and initiative is taken
New wheat germplasm with the excellent character of shore wheat enriches the hereditary basis of wheat breeding, excavates Resistant germplasm, formulates germplasm abundant
Material is the effective way and important directions for carrying out genetic improvement of wheat.The mistake that wheat inhereditary material is shifted to common wheat in shore
Cheng Zhong, the precise Identification of shore wheat inhereditary material and tracking are important and urgent work.These SSR markers and EST-STS label
Shore McGee can be amplified into the homologous group's addition line of Part VII in the derivative homologous group of offspring first part of common wheat-shore wheat
Because of the specific fragment of group, the transfer importeding into common wheat genetic background for shore wheat favorable genes provides technical support.
Establishing shore wheat genome specific molecular marker is identification, determines, tracks near isogenic wheat line inhereditary material the most
Effective method.Therefore, the major technique of shore wheat genome specific molecular marker is established are as follows: draw using SSR and EST-STS label
Object amplifies corresponding DNA band in China spring, common wheat 7182 and shore wheat, by China spring, common wheat 7182 and
The band of shore wheat compares, and filters out shore wheat genome specific band, thus can filter out shore wheat genome specific SSR marker and
EST-STS label.Therefore these special primers can be amplified into band in the derivative progeny material of wheat-shore wheat, to verify this
The specificity of a little SSR primers and EST-STS primer pair shore wheat inhereditary material.
Abbreviation and Key Term definition
SSR: simple sequence repeat marker, SSR are that have using 1~6 base as the tandem repetitive sequence of basic unit
Relatively rich state property and it is randomly distributed in whole gene group, according to the difference of recurring unit's size, sequence and copy number
Length polymorphism is presented.SSR technology is amplified according to the both ends sequence design specific primer of repetitive sequence by PCR reaction
The PCR product of different length.
EST-STS: EST, EST refer to from the cDNA library of different tissue sources random selected clone into
The expressed sequence segment for being about 300~400bp that the end of row 5 ' or 3 ' end sequencings obtain, number then correspond to its representative
Gene expression copy number, gene expression number is more, and corresponding cDNA clone is more, therefore can to the sequencing analysis of clone
To understand the gene expression abundance of gene.The technology path being consequently formed in recent years is widely used in gene identification, draws gene table
Up to map, the research fields such as new gene are found, and achieve remarkable effect.
Summary of the invention
The purpose of the present invention is to provide a kind of shore wheat genome specific molecular marker primer and its applications.By SSR and
EST-STS labeled primer is expanded, convenient for distinguishing the difference of shore wheat and China spring and common wheat 7182.The present invention is mentioned
The molecular labeling of confession can be used as the useful supplement of the prior art.
Itself the specific technical proposal is:
A kind of shore wheat genome specific molecular marker primer, including 47 kinds of SSR label primers and 119 kinds of EST-STS labels
Primer, 47 kinds of SSR label primers specifically: Xbarc59, Xwmc166 (9g1F), Xcfd0156, Xgwm314,
Xgwm271、Xcft3374、Xcfd190、Xcfd219、Xcfd31、Xcfd39、Xcfd71、Xcwm532、Xcfd156、
Xgdm0136、Xgpw5195、Xgpw7244、Xgwm518、Xwmc233(5b1.2F)、Xwmc27、Xgpw5195-1B、
Xwmc522(22h5F)、Xwmc533(26h1F)、Xwmc85-1B、Xwmc631、Xwmc702、Xwmc817、 Xwmc93
(6h12F)、Xwmc96(7d2F)、Xgwm264-1B、Xwmc471(25e1F)、Xwmc473(25e2F)、 Xgwm0249、
Xgwm0265、Xwmc734、Xcfd35、Xwmc397、Xgpw4095、Xgpw5237、Xmag1932、 Xmag1809、Xmag834、
Xmag3056, Xmag1441, Xmag3137, Xmag3284, Xmag1682, Xmag3318,119 kinds of EST-STS label draw
Object specifically: BE498572, BE426274, BF291740, BE406901, BE497107, CD490579, BG262410,
BM134486、BE425354、BE443071、BE443797、BE637935、 BE490643、BE471127、BE403153、
BF293581、BF201704、BE403214、BE426257、BE352611、 BE497177、BE499900、BE445925、
BE403867、BF291549、BF473049、BG607666、BQ166400、 CD453617、CD373543、CD373500、
CD452643、CD491981、CD373528、CD452846、BF484028、 BF474569、BF429122、BE590695、
BE490237、BE407068、BE499071、BG264080、BQ171235、 BQ159493、BQ167013、CD452736、
BQ159475、CD454353、CD454198、BF485285、BF484133、 BE499843、BE404437、BE398399、
BE637664、BE423141、CD373513、CD454706、BM135247、 BE443103、BG604819、BE406996、
BE499327、BE406901、BF291787、BE443796、BE497584、 BE495292、BQ162235、BE404332、
BQ169707、BQ160526、CD490485、CD373475、BF473348、 CD454575、BQ169491、BF291730、
BM137713、CD452844、BE605103、BE637806、CD453612、 BE606250、BF429203、CD454173、
BM134465、BE446061、BE404973、CD373484、BE444811、 BE591142、BE444644、BG604865、
CD491981、BF482781、BF482530、BE426692、BE591737、 BE637663、BG274576、BE591127、
BQ168298、BG606097、BE495150、BE405518、BF485168、BE495786、BE405749、BE518255、
BE443691,BE404963,BF202706,BF485078,BE490599, BE606392,BF473020,BE638039.Ability
Field technique personnel can be according to above-mentioned primer pair title from PCR primer website (http://wheat.pw.usda.gov/SNP/
New/pcr_primers.shtml it) or in URGI (https: //wheat-urgi.versailles.inra.fr/) obtains
The details of above-mentioned 166 pairs of primers, such as chromosome location and nucleotide sequence.
Table 1
Compared with prior art, beneficial effects of the present invention:
Molecular labeling provided by the present invention can be used as the useful supplement of the prior art, can help molecular selection breeding
Cheng Zhong, fast and effeciently tracks the inhereditary material of shore wheat, at the same be also beneficial to by the excellent genes of shore wheat and merit to by
The transfer of body Common Wheat Varieties, accelerates breeding process, improves the Breeding Efficiency and quality of new varieties.
A kind of application of shore wheat genome specific molecular marker primer during carrying shore wheat inhereditary material cultivar identification.
SSR primer and EST-STS primer mark are subjected to PCR amplification, concrete application method is as follows:
(1) amplification reaction system, 10 μ L of bulking property include following component:
(2) amplification program:
(3) whole process carries out on S1000 type thermal cycler Bio-Rad, California, USA.
Compared with prior art, beneficial effects of the present invention:
The present invention has using 47 kinds of SSR primers and 119 kinds of EST primers by the method for polymerase chain reaction (PCR)
Effect distinguishes the derivative offspring of common wheat and common wheat and nearly edge species shore wheat.
Detailed description of the invention
Fig. 1 is the expanding effect of primer material to be tested (arrow meaning is special target fragment)；Wherein 1. China spring；
2.7182；3. E. elongata；4. Thinopyrum intermedium；5. quasi- roegneria kamoji；6. psathyrostachys huashanica；7. shore wheat；8. rye.
Specific embodiment
Technical solution of the present invention is described in more detail with specific embodiment with reference to the accompanying drawing.
A kind of shore wheat genome specific molecular marker primer, including 47 kinds of SSR label primers and 119 kinds of EST-STS labels
Primer, 47 kinds of SSR label primers.
Embodiment
(1) extraction of DNA
The extraction of the DNA such as China spring, common wheat 7182 and shore wheat is using CTAB method to shore wheat young leaflet tablet full genome
Group DNA is extracted, and is modified slightly (Saghai-maroof et al.1984).
A. the steel ball that 2 sterilize is put into the centrifuge tube of 2m L sterilizing, the appropriate young leaflet tablet of clip is put into centrifuge tube
In, it is subsequently placed into 10min in liquid nitrogen.
B. centrifuge tube is taken out, is placed on high-throughput tissue grinder and shakes 2min, until blade is in powdered.
C. the CTAB Trace bio-element liquid 1000mL of 60 DEG C of preheatings is added, mixes, adds 400 μ L chloroforms, overturns, is mixed
It is even, steel ball is sucked out, 60 DEG C of water-bath 10min take out centrifuge tube ice bath 10min.
D.10000rpm it is centrifuged 5min, 700 μ L supernatants is drawn into the centrifuge tube of 1.5mL, the different of same volume is added
Propyl alcohol slowly overturns for several times, stands 10min.
E.10000rpm it is centrifuged 5min, outwells supernatant, 70% ethyl alcohol, 200 μ L is added and rinses, alcohol is removed in centrifugation, weight
It is 3 times multiple.
F. it puts in superclean bench, air-dries 12h, 500 μ L1 × TE (100mMTris-Hcl, 10mM EDTA, PH is added
=8.0) it dissolves.
After completely dissolution, 1.8 μ LRnaseA (10mgu are added in G.DNA-1), it mixes, 37 DEG C of water-bath 30min.
H. 500 μ L phenol/chloroforms/penta ethyl alcohol (25:24:1) is added, mixes, 8000rpm, 4 DEG C of centrifugation 10min.
I. Aspirate supernatant is transferred in the centrifuge tube of another sterilizing, and 50 μ LNaOAc (3molL are added-1PH=5.2),
It mixes, adds the dehydrated alcohol (- 20 DEG C) of 1mL, mix, be centrifuged after precipitating, outwell supernatant.
J. 70% ethyl alcohol of -20 DEG C of use pre-coolings rinse 2~3 times, are put into superclean bench and air-dry 12h, be added 100 μ L 1 ×
TE dissolution is spare.
(2) PCR reacts
A. amplification reaction system (10 μ L of bulking property) includes following component:
B. amplification program:
C. whole process carries out on S1000 type thermal cycler (Bio-Rad, California, USA).
(3) native polyacrylamide gel electrophoresis
A. glue, electrophoresis: being wiped clean glass plate with dehydrated alcohol, dried, and utilizes the fixed glass of gum-making rack, adhesive tape, clip
Glass plate, by 40m L polyacrylamide gel mother liquor (Acr Bis=37.5:1, w/w), the ammonium persulfate of 400 μ L10%, 40 μ
LTEMED is mixed, and is poured into glass plate, is slowly inserted into comb, is stood, comb is pulled up after solidification, lavage specimens hole is put into electrophoresis
In slot.2 μ L5 × Loading dye buffer are added in pcr amplification product, mixes, 5 μ L is successively loaded into loading wells.
2.5~3.0h of electrophoresis under 160 V constant pressures.
B. silver staining, development: by gel from being removed on glass plate in the silver nitrate solution for being put into 0.1%, shaking table jog 5min,
It is put into distilled water and rinses 30s, be then transferred into developer solution (1.5% sodium hydroxide, 0.4% formaldehyde), shaking table concussion, band
It is placed again into after clear in distilled water and stops development, record is taken pictures.
The foregoing is only a preferred embodiment of the present invention, the scope of protection of the present invention is not limited to this, it is any ripe
Know those skilled in the art within the technical scope of the present disclosure, the letter for the technical solution that can be become apparent to
Altered or equivalence replacement are fallen within the protection scope of the present invention.
Claims (3)
1. a kind of shore wheat genome specific molecular marker primer, which is characterized in that these primers amplify in the wheat genome of shore
Specific band, in wheat and homeologous kind storeroom, there were significant differences, including 47 kinds of SSR label primers and 119 kinds of EST-
STS labeled primer, 47 kinds of SSR label primers specifically: Xbarc59, Xwmc166 (9g1F), Xcfd0156, Xgwm314,
Xgwm271、Xcft3374、Xcfd190、Xcfd219、Xcfd31、Xcfd39、Xcfd71、Xcwm532、Xcfd156、
Xgdm0136、Xgpw5195、Xgpw7244、Xgwm518、Xwmc233(5b1.2F)、Xwmc27、Xgpw5195-1B、Xwmc522
(22h5F)、Xwmc533(26h1F)、Xwmc85-1B、Xwmc631、Xwmc702、Xwmc817、Xwmc93(6h12F)、Xwmc96
(7d2F)、Xgwm264-1B、Xwmc471(25e1F)、
Xwmc473(25e2F)、Xgwm0249、Xgwm0265、Xwmc734、Xcfd35、Xwmc397、Xgpw4095、
Xgpw5237、Xmag1932、Xmag1809、Xmag834、Xmag3056、Xmag1441、Xmag3137、Xmag3284、
Xmag1682, Xmag3318,119 kinds of EST-STS labeled primers specifically: BE498572, BE426274, BF291740,
BE406901、BE497107、CD490579、BG262410、BM134486、BE425354、BE443071、BE443797、
BE637935、BE490643、BE471127、BE403153、BF293581、BF201704、BE403214、BE426257、
BE352611、BE497177、BE499900、BE445925、BE403867、BF291549、BF473049、BG607666、
BQ166400、CD453617、CD373543、CD373500、CD452643、CD491981、CD373528、CD452846、
BF484028、BF474569、BF429122、BE590695、BE490237、BE407068、BE499071、BG264080、
BQ171235、BQ159493、BQ167013、CD452736、BQ159475、CD454353、CD454198、BF485285、
BF484133、BE499843、BE404437、BE398399、BE637664、BE423141、CD373513、CD454706、
BM135247、BE443103、BG604819、BE406996、BE499327、BE406901、BF291787、BE443796、
BE497584、BE495292、BQ162235、BE404332、BQ169707、BQ160526、CD490485、CD373475、
BF473348、CD454575、BQ169491、BF291730、BM137713、CD452844、BE605103、BE637806、
CD453612、BE606250、BF429203、CD454173、BM134465、BE446061、BE404973、CD373484、
BE444811、BE591142、BE444644、BG604865、CD491981、BF482781、BF482530、BE426692、
BE591737、BE637663、BG274576、BE591127、BQ168298、BG606097、BE495150、BE405518、
BF485168、BE495786、BE405749、BE518255、BE443691、BE404963、BF202706、BF485078、
BE490599、BE606392、BF473020、BE638039。
2. a kind of application of shore wheat genome specific molecular marker primer during carrying shore wheat inhereditary material cultivar identification.
3. shore wheat genome specific molecular marker primer according to claim 1 is carrying shore wheat inhereditary material cultivar identification
Application in the process, which is characterized in that SSR primer and EST-STS primer mark are subjected to PCR amplification, concrete application method is such as
Under:
(1) amplification reaction system, 10 μ L of bulking property include following component:
(2) amplification program:
(3) whole process carries out on S1000 type thermal cycler Bio-Rad, California, USA.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154286A (en) * | 2011-04-14 | 2011-08-17 | 中国科学院遗传与发育生物学研究所 | Wheat expressed sequence tag (EST) sequence-based specific molecular markers for chromosomes from 1R to 7R of rye and use thereof |
| CN104498483A (en) * | 2014-12-02 | 2015-04-08 | 山东省农业科学院作物研究所 | Aegilops searsii 2Ss chromosome molecular markers based on wheat and rice EST sequences and applications |
-
2018
- 2018-09-06 CN CN201811035231.0A patent/CN109112229A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154286A (en) * | 2011-04-14 | 2011-08-17 | 中国科学院遗传与发育生物学研究所 | Wheat expressed sequence tag (EST) sequence-based specific molecular markers for chromosomes from 1R to 7R of rye and use thereof |
| CN104498483A (en) * | 2014-12-02 | 2015-04-08 | 山东省农业科学院作物研究所 | Aegilops searsii 2Ss chromosome molecular markers based on wheat and rice EST sequences and applications |
Non-Patent Citations (1)
| Title |
|---|
| 孙文静: "滨麦草特异标记的筛选及抗条锈病小滨麦种质系鉴定", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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