CN109105150A - A kind of production method improving Antrodia camphorata effective component - Google Patents
A kind of production method improving Antrodia camphorata effective component Download PDFInfo
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- CN109105150A CN109105150A CN201810987444.7A CN201810987444A CN109105150A CN 109105150 A CN109105150 A CN 109105150A CN 201810987444 A CN201810987444 A CN 201810987444A CN 109105150 A CN109105150 A CN 109105150A
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- antrodia camphorata
- pine
- linden
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The present invention provides a kind of production methods for improving Antrodia camphorata effective component, belong to the production field more particularly to a kind of breeding method for using pine genus plant to carry out Antrodia camphorata as host of Antrodia camphorata.The breeding method uses host of the Pinus trunk as Antrodia camphorata strain, it places it in the liquid culture medium containing corn victory peptide, and the air of rich oxygen content is passed through into the cavity of host's linden, the advantages of this technical solution is: 1. avoid the indiscriminate felling of trees to the wild cinnamomum kanehirai of child care class tree species, while the large-scale artificial cultivation of Antrodia camphorata also being made to realize that volume production provides excellent technique scheme；2. substantially increasing the Antrodia camphorata product polysaccharide body and total triterpene contents of artificial cultivation；3. accelerating the growth multiplying speed of Antrodia camphorata strain, shorten the period of artificial cultivation Antrodia camphorata.
Description
Technical field
The present invention relates to the production field of Antrodia camphorata more particularly to a kind of pinaceae plant is used to carry out Antrodia camphorata as host
Breeding method.
Background technique
Referred to as " antrodia ", scientific name is Antrodia camphorata to Antrodia camphorata, popular name cinnamomum kanahirai hay mushroom, red antrodia, mushroom in camphor tree,
Camphor tree mushroom, blood ganoderma lucidum etc. belong to Aphyllophorales, Polyporaceae, antrodia karst, perennial gill fungus mushroom, the entitled Antrodia of Latin
Camphorata is the novel species just published by biochemical boundary nineteen ninety.The growth district of Antrodia camphorata is Taiwan mountain area height above sea level 450
Between~2000 meters of mountain forests, it is only grown in the rotten heartwood inner wall of the Taiwan above cinnamomum kanehirai trunk of distinctive a century.Cinnamomum kanehirai timber
Surface moisture, Antrodia camphorata acquisition is not easy, in addition wild cinnamomum kanehirai is as Taiwan child care class tree species, it is big through the late Qing Dynasty to Japanese-occupation period
Amount felling has remained little.And Antrodia camphorata has effects that the mystery such as liver protection, anticancer, reinforced immunological, antiallergy, reducing blood lipid of detoxifying,
Usually enjoy the good reputation of " king of medicine sesame ", " ruby in the forest of Taiwan ".Therefore, the personal value of Antrodia camphorata is with wild cinnamomum kanehirai
Die-off and reach prosperous.
About 225 kinds of the separated obtained compound from Antrodia camphorata in 2012.These compounds include macromolecular compound
(albumen, nucleic acid, polysaccharide etc.), (lignin, aromatic compound, benzoquinones, maleic acid and succinic acid are derivative for small molecule compound
Object etc.), terpenoid (monoterpene, diterpene, lanostane-type triterpene, lumistane type triterpene, steroidal etc.), ucleotides chemical combination
Object (base, nucleosides etc.), fatty acid and aliphatic ester etc..Wherein, triterpenes and polysaccharide body are to keep Antrodia camphorata status much high
In the key component of ganoderma lucidum and the main target extraction of substance of Antrodia camphorata artificial culture.
The artificial culture of Antrodia camphorata can substantially be divided into cultivation basswood method, plate method, three kinds of solution fermentation.It utilizes
The original host's cinnamomum kanehirai linden of Antrodia camphorata is that culture medium cultivates Antrodia camphorata, can obtain ingredient identical with wild Antrodia camphorata and function
Effect, but incubation time is up to 2~3 years, and due to wild cinnamomum kanehirai rare numbers, and its precious, this culture means cost
It is too high.Second Antrodia camphorata strain is subjected to Mycelium culture in space packet, fibre object, carbohydrate and five cereals are contained in space packet
Coarse cereals class etc., the Antrodia camphorata product of acquisition is similar to wild Antrodia camphorata shape but ingredient is totally different, and incubation time about 3 months, culture
Cost is slightly higher.The third be using 500 liters even tonne or more liquid fermentation slot carry out strain liquid ferment to collect bacterium
Filament, incubation time is short, and about 7~14 days or so, the method can not obtain the distinctive triterpenes of wild Antrodia camphorata.
Antrodia camphorata breeding method disclosed in Chinese invention patent CN104472210B combines the first and the third cultivation
Antrodia camphorata strain is placed on Antrodia camphorata linden and is tentatively cultivated by method, and mycelium to be grown is placed on liquid fermentation and culture
Fermented and cultured is carried out in base, incubation time is 8~12 days, obtains finished product Antrodia camphorata, the technical solution of this patent and the first side
Method is equally using very rare cinnamomum kanehirai linden as host, and under the promotion for avoiding illegal mining cinnamomum kanehirai, the method is unsuitable for
Carry out volume production popularization and large-scale practical application, and the obtained Antrodia camphorata of the technical solution is compared with wild Antrodia camphorata,
Form does not explain with effective component.
Summary of the invention
For this reason, it may be necessary to a kind of production method for improving Antrodia camphorata effective component be provided, to solve to improve artificial culture Cinnamomum kanahirai hay
Sesame effective active composition shortens the problem of production cycle and reduction production cost.
To achieve the above object, a kind of production method for improving Antrodia camphorata effective component is inventor provided, including following
Step:
Prepare pine linden: taking the two leaves pine that tree-walk is 20~40cm, the trunk of three leaves pine or five leaves pine, be cut into length
Degree is the linden of 40~50cm, obtains pine linden；
Preparation inoculation former base: it from an end face of the pine linden along the axially drilled cavity of the pine linden, obtains
To inoculation former base；
Preparation inoculation base: the inoculation former base being dried in vacuo, is sterilized, is placed in culture medium, the culture medium packet
The corn for being 5~15% containing weight percent wins peptide, obtains inoculation base；
Inoculation: Antrodia camphorata bacterium solution is implanted into the cavity；
Sporophore growth management and harvesting: keeping the growth temperature of Antrodia camphorata bacterium solution at 20~25 DEG C, logical every 8~10 days
Hyperoxia air, a duration of ventilation are 2h, and ventilation flow rate is 80~150vvm, is ventilated 4~6 times, and it is real to obtain Antrodia camphorata
Body is gathered in.
The chemical component of pinaceae plant is more complex, but its common feature is mostly to lack biology containing resin and volatile oil
Alkali lacks biflavone.Resin is stored in resin canal, is coexisted with volatile oil.Organic acid (contains in such as rosin containing there are many in resin
Have 90% or more resin acid), there are also resinol, resin ester and a large amount of resin hydro carbons.Volatile oil is contained in needle and resin,
Containing there are many alkenes in volatile oil.Pine tree is most distinct feature is that leaf is at needle-shaped, and normal 2 needle, 3 needles or 5 needles are a branch of, referred to as two leaves
Pine, three leaves pine and five leaves pine, 2 needle of leaf such as Chinese pine, masson pine, Pinus taiwanesis is a branch of, and 3 needle of leaf of lacebark pine is a branch of, Korean pine, the Huashan
Pine, 5 needle of leaf of five-leaved pine are a branch of.In the implementation of the present invention, inventor has found that two leaves pine, three leaves pine and five leaf pines etc. are loose
Section plant, the taxodiaceae plants such as yew, Taiwania flousiana have similar an aroma substance and volatile oil as cinnamomum kanehirai, but yew,
The taxodiaceae plants such as Taiwania flousiana can make to absorb the intracorporal toxicant of host in the cultivating process of Antrodia camphorata strain as host, influence ox
The quality of antrodia product, therefore, host of the present invention using the linden of two leaves pine, three leaves pine or five leaves pine as Antrodia camphorata is led to
Multiple verification experimental verification is crossed, the Antrodia camphorata polysaccharide body and total triterpene contents obtained by the technical solution is suitable with wild Antrodia camphorata, but
Its toxicity is not will increase.
It is that 5~15% corns victory peptide is advantageous in that weight percent is added in culture medium, is made using this programme production
Polysaccharide body and the content of total triterpene are suitable with wild Antrodia camphorata in Antrodia camphorata fructification, promoted artificial cultivation Antrodia camphorata quality and
Health value.
Further, the hyperoxia air is the air of oxygen content 80~100%.
In traditional Antrodia camphorata artificial culture method, just the concentration of fermentation and post-fermentation stage oxygen is controlled 5~15% more,
And grope to verify repeatedly through overtesting, inventor has found the air for being passed through oxygen content 80~100%, and Antrodia camphorata strain is in host linden
Nutrition that can rapidly in speed sorption enhanced culture medium on wood, and then shoot up as fructification.
Further, the cavity is 1.5~3cm of diameter, the cylindrical cavity of deep 12~16cm.
The effect of cavity is to provide growing environment for Antrodia camphorata bacterium solution, this growing environment is not the bigger the better, also simultaneously
It is non-the smaller the better, it is detected by many experiments, 1.5~3cm of diameter, the cylindrical cavity of deep 12~16cm can make two leaves pine, three
The linden rosin smell of leaf pine or five leaves pine releases optimum concentration range needed for concentration is in Antrodia camphorata growth.
Further, the number of cavities is 1~15.
Cavity is as implantation Antrodia camphorata bacterium solution and is passed through the channel of hyperoxia air, from pine linden one end along its axial direction to another
When one end drills through, the content of nutritional ingredient in the thickness and culture medium of linden diameter can be regarded, drills through 1~15 cavity, cavity
Distribution keeps interval uniformly as far as possible, can guarantee the strain and oxygen concentration that reach pine linden side and each point in end face in this way
Relative equilibrium, the culture medium that pine linden bottom is impregnated are transported to the battalion of each point in linden side and end face by vascular bundle absorption
Form point also relative equilibrium.
Further, the culture medium percentage also includes following ingredient: 35% potato extracting solution,
20% glucose, 0.2%KH2PO4, 0.1%MgSO4·7H2O, 0.1% vitamin B1, 0.1% vitamin B2。
It is different from the prior art, above-mentioned technical proposal has the advantages that 1. use two leaves pine, three leaves pine or five leaves pine
Host of the trunk as artificial cultivation Antrodia camphorata avoids indiscriminate felling of trees of the people to the wild cinnamomum kanehirai of child care class tree species, simultaneously
Also the large-scale artificial cultivation of Antrodia camphorata is made to realize that volume production provides excellent technique scheme；2. by the host linden containing Antrodia camphorata bacterium solution
Wood is placed in the culture medium of the victory peptide containing corn, substantially increases Antrodia camphorata finished product polysaccharide body and total triterpene contents；3. toward host linden
It is passed through hyperoxia air in the cavity of wood, the growth multiplying speed of Antrodia camphorata strain is accelerated, shortens artificial cultivation Antrodia camphorata
Period.
Detailed description of the invention
Fig. 1, which is that the present embodiment is a kind of, is passed through hyperoxia air jet flow in the culture medium for winning peptide containing corn using five leaf pine lindens
The schematic diagram of Antrodia camphorata；
Fig. 2, which is that the present embodiment is a kind of, is passed through hyperoxia in the culture medium for winning peptide containing corn using two leaves pine or three leaf pine lindens
The schematic diagram of air jet flow Antrodia camphorata.
Description of symbols:
1, it is inoculated with base；
2, cavity；
3, culture medium；
4, hyperoxia air vent.
Specific embodiment
Technology contents, construction feature, the objects and the effects for detailed description technical solution, below in conjunction with specific reality
It applies example and attached drawing is cooperated to be explained in detail.
The measurement of total triterpene contents and polysaccharide body content is UV-VIS spectrophotometry.
Embodiment one
Antrodia camphorata strain purchase is deposited in country from Taiwan legendary god of farming's fungal organism Technology Co., Ltd., bacterium numbering AC001
Juncao Engineering Technical Research Centre.
1. being passed through hyperoxia air jet flow Antrodia camphorata in the culture medium of the victory peptide containing corn using five leaf pine lindens
Referring to Fig. 1, the present embodiment provides a kind of lifes for using five leaf pine lindens as host's raising Antrodia camphorata effective component
Production method, comprising the following steps:
Prepare pine linden: taking tree-walk is the trunk of five leaf of 20cm pine, is cut into the linden that length is 40cm, obtains five
The leaf loosely wooden linden；
Preparation inoculation former base: from the center of an above-mentioned five leaf loosely end face of the wooden linden along the pine linden
An axially drilled diameter is the cavity 2 of 3cm, depth 16cm, obtains inoculation former base；
Preparation inoculation base: the inoculation former base being dried in vacuo, after ultraviolet lamp radiation sterilization, is placed in culture medium,
3 percentage of culture medium, comprising 0~15% corn win peptide, 35% potato extracting solution, 20% glucose,
0.2%KH2PO4, 0.1%MgSO4·7H2O, 0.1% vitamin B1, 0.1% vitamin B2, obtain inoculation base 1；
Inoculation: Antrodia camphorata bacterium solution is implanted into the cavity of the inoculation base with stainless steel tube, the Antrodia camphorata bacterium solution is ox
The seed liquor that antrodia strain is activated through conventional method, spread cultivation, and it is passed through the hyperoxia air that oxygen content is 80%；
Sporophore growth management and harvesting: keeping the growth temperature of Antrodia camphorata at 22 DEG C, led to one from blow vent 4 every 10 days
The secondary hyperoxia air, duration of ventilation are 2h, ventilation flow rate 100vvm, are ventilated 6 times, when observing to go out on pine linden
Now gathered in the fructification of Chinese red to get Antrodia camphorata product is arrived.
2. being passed through air jet flow Antrodia camphorata in the culture medium of the victory peptide containing corn using five leaf pine lindens
It is passed through unlike hyperoxia air jet flow Antrodia camphorata in the culture medium of the victory peptide containing corn from using five leaf pine lindens
It in sporophore growth management and harvesting steps, keeps the growth temperature of Antrodia camphorata at 22 DEG C, led to one from blow vent 4 every 10 days
Secondary normal air, a duration of ventilation are 2h, ventilation flow rate 100vvm, ventilate 6 times, are in when observing on pine linden
The fructification of Chinese red is gathered in get Antrodia camphorata product is arrived.
The Antrodia camphorata cultivated in the culture medium of different content corn victory peptide is sampled in each ventilation, is detected above-mentioned
Total triterpene and polysaccharide body content in two kinds of obtained Antrodia camphoratas of cultural method, as a result as shown in Table 1 and Table 2.
Table 1 adds total triterpene contents (unit: g/100g) in different content corn victory five leaf pine of peptide culture Antrodia camphorata
Table 2 adds polysaccharide body content (dry weight, unit: g/ in different content corn victory five leaf pine of peptide culture Antrodia camphorata
100g)
From it was found from the data of Tables 1 and 2: 1. in the culture medium of same amount corn victory peptide, are passed through hyperoxia air jet flow
Obtained Antrodia camphorata, total triterpene and polysaccharide body content, which are above, is passed through the Antrodia camphorata that normal air culture obtains；2. equally all
It is the Antrodia camphorata for being passed through hyperoxia air jet flow and obtaining, total triterpene and polysaccharide body content with corn victory peptide content in culture medium
Increase and increases；3. comparing from sample detecting data it is found that day sample detecting obtains from the 10th day to the 40th total triterpene and more
Sugared body content is in increasing trend, and total triterpene and polysaccharide body changes of contents are little after the 40th day to the 60th day.In summary, it utilizes
Five leaf pine linden cultures containing corn victory peptide culture medium in cultivate Antrodia camphorata optimal conditions are as follows: be passed through oxygen content be 80~
100% hyperoxia air, the content of corn victory peptide is 10~15% in culture medium, and culture starts to harvest for 40 days or so.
Embodiment two
Antrodia camphorata strain purchase is deposited in country from Fuzhou Green Valley Biopharmaceuticals Technology Research Institute, bacterium numbering AC1404
Juncao Engineering Technical Research Centre.
Referring to Fig. 2, the present embodiment provides a kind of lifes for using two leaf pine lindens as host's raising Antrodia camphorata effective component
Production method, comprising the following steps:
Prepare pine linden: taking tree-walk is the trunk of bis- leaf of 30cm pine, is cut into the linden that length is 50cm, obtains two
The leaf loosely wooden linden；
Preparation inoculation former base: from an end face of the above-mentioned two leaf loosely wooden linden along the axial to another of the pine linden
End face drills through the cavity 2 that 8 diameters are 2cm, depth 14cm, obtains inoculation former base；
Preparation inoculation base: the inoculation former base being dried in vacuo, after ultraviolet lamp radiation sterilization, is placed in culture medium,
3 percentage of culture medium, comprising 15% corn win peptide, 35% potato extracting solution, 20% glucose, 0.2%
KH2PO4, 0.1%MgSO4·7H2O, 0.1% vitamin B1, 0.1% vitamin B2, obtain inoculation base 1；
Inoculation: Antrodia camphorata bacterium solution is implanted into the cavity of the inoculation base with stainless steel tube, the Antrodia camphorata bacterium solution is ox
The seed liquor that antrodia strain is activated through conventional method, spread cultivation, and the air that oxygen content is 100% is passed through from blow vent 4；
Sporophore growth management and harvesting: keeping the growth temperature of Antrodia camphorata at 25 DEG C, the logical primary hyperoxia every 8 days
Air, a duration of ventilation are 2h, ventilation flow rate 80vvm, are ventilated 5 times, are occurred when observing on pine linden in Chinese red
Fructification to get arrive Antrodia camphorata product, gathered in.
Embodiment three
Antrodia camphorata strain purchase is deposited in state's house fungus from Fuzhou Green Valley Biopharmaceuticals Technology Research Institute, bacterium numbering AC620
Careless Engineering Technical Research Centre.
Unlike embodiment two, pine linden used by the present embodiment is the trunk of three leaves pine, and trunk diameter is
40cm is highly 40cm, and the cavity drilled through is diameter 1.5cm, depth 12cm, and quantity is 15, beautiful comprising 5% in the culture medium
Rice victory peptide, 35% potato extracting solution, 20% glucose, 0.2%KH2PO4, 0.1%MgSO4·7H2O, 0.1% vitamin B1、
0.1% vitamin B2, it is passed through the air that oxygen content is 90% in incubation, in fructification incubation, keeps Antrodia camphorata
Growth temperature is at 20 DEG C, and every 9 days, the logical primary hyperoxia air, a duration of ventilation were 2h, ventilation flow rate 150vvm, leads to
Gas 4 times.
By adopting the above technical scheme, it is implanted in pine linden cavity from Antrodia camphorata bacterium solution to Antrodia camphorata fructification and harvests,
The period of production shortened to 40~60 days from culture period 2~3 years of wild Antrodia camphorata, every 100g Antrodia camphorata fructification it is total
Triterpene content may be up to 9.3g, and polysaccharide body content is up to 4.9g, closest with wild Antrodia camphorata total triterpene and polysaccharide body content.
It should be noted that being not intended to limit although the various embodiments described above have been described herein
Scope of patent protection of the invention.Therefore, it based on innovative idea of the invention, change that embodiment described herein is carried out and is repaired
Change, or using equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it directly or indirectly will be with
Upper technical solution is used in other related technical areas, is included within scope of patent protection of the invention.
Claims (5)
1. a kind of production method for improving Antrodia camphorata effective component, which comprises the following steps:
Prepare pine linden: taking the two leaves pine that tree-walk is 20~40cm, the trunk of three leaves pine or five leaves pine, being cut into length is
The linden of 40~50cm obtains pine linden；
Preparation inoculation former base: it from an end face of the pine linden along the axially drilled cavity of the pine linden, is connect
Kind former base；
Preparation inoculation base: the inoculation former base being dried in vacuo, is sterilized, is placed in culture medium, and the culture medium includes weight
It measures the corn that percentage is 5~15% and wins peptide, obtain inoculation base；
Inoculation: Antrodia camphorata bacterium solution is implanted into the cavity；
Sporophore growth management and harvesting: keeping the growth temperature of Antrodia camphorata bacterium solution at 20~25 DEG C, logical primary every 8~10 days
Hyperoxia air, a duration of ventilation are 2h, and ventilation flow rate is 80~150vvm, ventilate 4~6 times, obtain Antrodia camphorata fructification, into
Row harvesting.
2. production method according to claim 1, which is characterized in that the hyperoxia air is oxygen content 80~100%
Air.
3. production method according to claim 1, which is characterized in that the cavity is 1.5~3cm of diameter, deep 12~
The cylindrical cavity of 16cm.
4. production method according to claim 3, which is characterized in that the number of cavities is 1~15.
5. production method according to claim 1, which is characterized in that the culture medium percentage also includes
Following ingredient: 35% potato extracting solution, 20% glucose, 0.2%KH2PO4, 0.1%MgSO4·7H2O, 0.1% vitamin
B1, 0.1% vitamin B2。
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
| CN103918472A (en) * | 2013-01-15 | 2014-07-16 | 漳州美利德生物工程有限公司 | Antrodia camphorata basswood cultivation method |
| CN104402575A (en) * | 2014-10-23 | 2015-03-11 | 庆元县秋平真菌研究所 | Antrodia medium formula and antrodia cultivation method |
| CN104855137A (en) * | 2015-05-22 | 2015-08-26 | 福州惠生食用菌有限公司 | Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea |
| CN108611268A (en) * | 2018-05-08 | 2018-10-02 | 浙江海正药业股份有限公司 | A kind of production spore device for simulating wild environment and its application in Antrodia camphorata |
-
2018
- 2018-08-28 CN CN201810987444.7A patent/CN109105150A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
| CN103918472A (en) * | 2013-01-15 | 2014-07-16 | 漳州美利德生物工程有限公司 | Antrodia camphorata basswood cultivation method |
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
| CN104402575A (en) * | 2014-10-23 | 2015-03-11 | 庆元县秋平真菌研究所 | Antrodia medium formula and antrodia cultivation method |
| CN104855137A (en) * | 2015-05-22 | 2015-08-26 | 福州惠生食用菌有限公司 | Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea |
| CN108611268A (en) * | 2018-05-08 | 2018-10-02 | 浙江海正药业股份有限公司 | A kind of production spore device for simulating wild environment and its application in Antrodia camphorata |
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