CN109095679A - A kind of drinking water disinfection purification system and its technique handling antibiotic resistance bacterium and resistant gene - Google Patents
A kind of drinking water disinfection purification system and its technique handling antibiotic resistance bacterium and resistant gene Download PDFInfo
- Publication number
- CN109095679A CN109095679A CN201811292577.9A CN201811292577A CN109095679A CN 109095679 A CN109095679 A CN 109095679A CN 201811292577 A CN201811292577 A CN 201811292577A CN 109095679 A CN109095679 A CN 109095679A
- Authority
- CN
- China
- Prior art keywords
- drinking water
- resistant gene
- electrode
- electrochemical reactor
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 64
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 55
- 239000003651 drinking water Substances 0.000 title claims abstract description 44
- 235000020188 drinking water Nutrition 0.000 title claims abstract description 44
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 32
- 238000000746 purification Methods 0.000 title claims abstract description 29
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 96
- 238000001914 filtration Methods 0.000 claims abstract description 13
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims abstract description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 34
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical group [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 32
- 239000012528 membrane Substances 0.000 claims description 18
- 239000011780 sodium chloride Substances 0.000 claims description 17
- 229910003460 diamond Inorganic materials 0.000 claims description 16
- 239000010432 diamond Substances 0.000 claims description 16
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 16
- 229910052697 platinum Inorganic materials 0.000 claims description 16
- 238000001471 micro-filtration Methods 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 10
- 230000008859 change Effects 0.000 claims description 6
- 230000015271 coagulation Effects 0.000 claims description 6
- 238000005345 coagulation Methods 0.000 claims description 6
- 230000001376 precipitating effect Effects 0.000 claims description 6
- 230000002459 sustained effect Effects 0.000 claims description 6
- 238000011282 treatment Methods 0.000 claims description 5
- 230000005518 electrochemistry Effects 0.000 claims description 4
- 238000001354 calcination Methods 0.000 claims description 3
- 229940075397 calomel Drugs 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 230000003834 intracellular effect Effects 0.000 abstract description 10
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 238000006056 electrooxidation reaction Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 27
- 230000000052 comparative effect Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 15
- 230000008676 import Effects 0.000 description 14
- 230000001954 sterilising effect Effects 0.000 description 12
- 240000000233 Melia azedarach Species 0.000 description 10
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 10
- 230000005611 electricity Effects 0.000 description 8
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 6
- 239000000460 chlorine Substances 0.000 description 6
- 229910052801 chlorine Inorganic materials 0.000 description 6
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical class O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 238000011529 RT qPCR Methods 0.000 description 4
- 239000004098 Tetracycline Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- -1 sulfamido Chemical class 0.000 description 4
- 101150024821 tetO gene Proteins 0.000 description 4
- 235000019364 tetracycline Nutrition 0.000 description 4
- 150000003522 tetracyclines Chemical class 0.000 description 4
- 229940040944 tetracyclines Drugs 0.000 description 4
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 3
- 230000008485 antagonism Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000005202 decontamination Methods 0.000 description 2
- 230000003588 decontaminative effect Effects 0.000 description 2
- 238000003487 electrochemical reaction Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000003411 electrode reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
- C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/76—Treatment of water, waste water, or sewage by oxidation with halogens or compounds of halogens
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/02—Non-contaminated water, e.g. for industrial water supply
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Physical Water Treatments (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention discloses a kind of drinking water disinfection purification systems and its technique for handling antibiotic resistance bacterium and resistant gene, and purification system includes ultraviolet ray disinfector, millipore filter and electrochemical reactor；Ultraviolet ray disinfector is equipped with drinking water water inlet, ultraviolet ray disinfector is sequentially connected millipore filter and electrochemical reactor by pipeline, electrochemical reactor is equipped with drinking water water outlet, connecting line between millipore filter and electrochemical reactor is equipped with T-type charge pipe, and T-type charge pipe is equipped with second level inlet；The present invention removes most of bacterium including antibiotic-resistant bacteria by ultraviolet irradiation, while removing intracellular resistant gene to a certain extent；Then pass through the resistance bacterium of micro porous filtration removal dead bacterial body and a small amount of work；Deliquescent extracellular resistant gene and a small amount of resistant gene intracellular in water are completely removed finally by the electrochemical oxidation system of low energy consumption；The present invention by water source resistance bacterium and resistant gene completely remove, ensured water supply security, had great application prospect.
Description
Technical field
The present invention relates to technical field of drinking water treatment more particularly to a kind of processing antibiotic resistance bacterium and resistant genes
Drinking water disinfection purification system and its technique.
Background technique
Although the application of antibiotic to the mankind brings many benefits, because of tolerant bacteria caused by abuse of antibiotics and
The pollution of resistant gene just threatens human health and ecological safety.It is estimated that the whole world is every year because antibiotic resistance is microbial
Death toll reaches hundreds of thousands people.Resistant gene in resistance bacterium can carry out in the environment propagation expansion by modes such as horizontal transfers
Dissipate, even if resistance bacterium is dead, exposed resistant gene also can long-term existence in the environment, and have and be transferred to other cause a disease
The ability of bacterium.Detect the presence of resistance bacterium and resistant gene in global range in various environment waters at present, wherein water
A certain amount of resistance bacterium and resistant gene are detected in source water and drinking water.Long-term intake resistance bacterium and resistant gene, will
Will lead to enteric bacteria and generate resistance, grave danger generated to human health, thus the sterilizing part antagonism bacterium of drinking water and
The removal effect of resistant gene generates important influence to human health.
However, the sterilization process of conventional waterworks can not effectively remove resistance bacterium and resistant gene.Although using
The sterilization dose of very high ultraviolet, chlorine or ozone can effectively kill resistance bacterium, but the place to go effect of antagonism gene is very
It is limited, while can also induce common bacteria and generate resistance, and chlorine and ozonization will lead to bacterial cell rupture, cause resistance
Gene releaser increases the risk of resistant gene propagation into water body instead.
Summary of the invention
In view of the above problems, it is high that it is an object of that present invention to provide a kind of removal rates, does not generate any by-product,
The drinking water purification system and its technique of operating cost low processing antibiotic resistance bacterium and resistant gene.
In order to achieve the above object, The technical solution adopted by the invention is as follows: a kind of processing antibiotic bacteria and antibiosis gene
Drinking water disinfection purification system, the disinfection and purification system include ultraviolet ray disinfector, millipore filter and electrochemical reaction
Device；The ultraviolet ray disinfector is equipped with drinking water water inlet, and ultraviolet ray disinfector is sequentially connected micro porous filtration by pipeline
Device and electrochemical reactor, the electrochemical reactor are equipped with drinking water water outlet, the millipore filter and electrification
It learns the connecting line between reactor and is equipped with T-type charge pipe, the T-type charge pipe is equipped with second level inlet.
Inlet and outlet on ultraviolet ray disinfector of the invention are arranged in sustained height, the electrochemical reaction
In sustained height, the water inlet of the millipore filter is arranged in bottom, water outlet for inlet and outlet setting on device
It is arranged at top.
Auxiliary electrode, reference electrode and working electrode, the auxiliary electrode are equipped in electrochemical reactor of the invention
The top of electrochemical reactor is set with reference electrode, and the bottom of electrochemical reactor is arranged in the working electrode.
A kind of drinking water disinfection purification process handling antibiotic bacteria and antibiosis gene provided by the invention, including walk as follows
It is rapid:
1) ultraviolet ray disinfector will be passed through by pretreated drinking water to handle, water inlet flow velocity is 100mL/min,
UV irradiation dose is 45~65mJ/cm2, time of contact 4s；
2) drinking water for obtaining step 1) is passed through in millipore filter and handles, wherein the filter in millipore filter
Membrane aperture is 0.15~0.25 μm；
3) sodium chloride solution is added into the drinking water for obtaining step 2), so that concentration of the sodium chloride in drinking water is protected
It holds in 0.1~0.2mmol/L.By the way that sodium chloride solution is added to increase electric conductivity, and a small amount of activity is generated by electrode reaction
Chlorine species and chlorine improve electrochemical oxidation efficiency, while also providing chlorine residue for water outlet；
4) drinking water for obtaining step 3) is passed through electrochemical reactor and handles, the operating voltage of electrochemical reactor
For 1~1.5V；
5) be by step 4) treated drinking water safety drinking water.
Pretreated drinking water in step 1) of the invention is drinking after coagulation, precipitating, filtration treatment
Water.
Working electrode of the present invention is boron-doped diamond electrode, and auxiliary electrode is platinum electrode, and reference electrode is saturation calomel electricity
Pole.
In step 2) of the invention, used micro-filtration, membrane filter are disposed through drying, calcination；At dry and calcination
Filter core, filter membrane after reason destroy not killed resistance bacterium and intracellular resistant gene, prevent secondary pollution.
The present invention has the advantages that drinking water disinfection purification system proposed by the present invention by improving ultraviolet irradiation agent in right amount
Amount efficiently removes most of bacterium including antibiotic-resistant bacteria, while removing intracellular resistance base to a certain extent
Cause；Then pass through the resistance bacterium of micro porous filtration removal dead bacterial body and a small amount of work；Finally by the electrochemistry of low energy consumption
Oxidation system completely removes deliquescent extracellular resistant gene and a small amount of resistant gene intracellular in water.The sterilization process can make
Resistance bacterium and resistant gene in source water are almost removed, and water supply security and human health have been ensured, in Water purification
It has great application prospect in engineering practice.
Although the component units of decontamination system proposed by the present invention, such as: ultraviolet disinfection, micro porous filtration and electrochemical disinfection
Unit has mentioned, but the combination and corresponding application method that the invention patent proposes in existing research
Multiple Classes of Antibiotics tolerant bacteria and resistant gene in source water can be removed to the maximum extent, do not generate any disinfection by-product
Object guarantees the tight security of effluent quality；With individual ultraviolet disinfection mode, micro porous filtration, electrochemical disinfection method and three
Other group technologies of person are compared, and the removal effect of decontamination system and method antagonism bacterium and resistant gene that this patent proposes is more
Good, operating cost is lower, and energy consumption is also lower, and not will increase resistant gene propagation and resistance bacterium and resistant gene it is secondary
Pollution.
Detailed description of the invention
Fig. 1 is the apparatus structure schematic diagram of purification system of the present invention；
Fig. 2 is the removal rate result of resistance bacterium and resistant gene in the final outflow water of the embodiment of the present invention and comparative example
Figure；
Fig. 3 is that resistance bacterium and resistant gene in final outflow water are applied after different ultraviolet irradiation intensity in the embodiment of the present invention
Removal rate result figure；
Fig. 4 is that resistance bacterium and resistance base in final outflow water are applied behind different millipore filter apertures in the embodiment of the present invention
The removal rate result figure of cause；
Fig. 5 is that resistance bacterium and resistance in final outflow water are applied after different electrochemical reactor voltages in the embodiment of the present invention
The removal rate result figure of gene.
Wherein, 1 drinking water water inlet, 2 ultraviolet ray disinfectors, 3UV tube power line, 4 millipore filters, the charging of 5T type
Pipe, 6 second level inlets, 7 electrochemical reactors, 8 connection power supply lines, 9 auxiliary electrodes and reference electrode, 10 working electrodes, 11 drinks
With water water outlet.
Specific embodiment
The present invention is described in further detail with specific embodiment for explanation with reference to the accompanying drawing.
Embodiment 1: the drinking water purification system of a kind of processing antibiotic resistance bacterium and resistant gene as shown in Figure 1, institute
The purification system stated includes ultraviolet ray disinfector 2, millipore filter 4 and electrochemical reactor 7；The ultraviolet ray disinfector 2
It is equipped with pretreated drinking water water inlet 1, ultraviolet ray disinfector 2 is sequentially connected millipore filter 4 and electrification by pipeline
Reactor 7 is learned, the electrochemical reactor 7 is equipped with drinking water water outlet 11, and the millipore filter 4 and electrochemistry are anti-
The connecting line between device 7 is answered to be equipped with T-type charge pipe 5, the T-type charge pipe 5 is equipped with second level inlet 6.
Embodiment 2: as shown in Figure 1, the inlet and outlet setting on ultraviolet ray disinfector 2 is in sustained height, it is described
Electrochemical reactor 7 on inlet and outlet setting in sustained height, the water inlet of the millipore filter 4 is arranged
In bottom, water outlet is arranged at top.
Embodiment 3: as shown in Figure 1, auxiliary electrode, reference electrode 9 and working electrode 10 are equipped in electrochemical reactor 7,
The top of electrochemical reactor 7 is arranged in the auxiliary electrode and reference electrode 9, and the working electrode 10 is arranged in electrification
Learn the bottom of reactor 7.
Embodiment 4: it using water outlet of the Nanjing waterworks after coagulation, precipitating, filtering as test water, is testing
Under the scale of room, the disinfection and purification system proposed through this patent is handled, it may be assumed that
1) test water enters Philip UV-17W ultraviolet sterilizer with the flow velocity of 100mL/min, and UV irradiation dose is
55mJ·cm-2, time of contact 4s；
2) water outlet enters Jiang great Lian Sheng tube micro porous filter device 3RS3 progress micro-filtration, and filter core is its matched import filter membrane system
At 0.2 μm of aperture；
3) sodium chloride solution of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min；
4) water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum
Electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V.
Meanwhile implementing comparative example, it is as follows:
Comparative example 1: test water enters Philip UV-17W ultraviolet sterilizer, ultraviolet irradiation with the flow velocity of 100mL/min
Dosage is 55mJcm-2, time of contact 4s.
Comparative example 2: test water with the flow velocity of 100mL/min enter Jiang great Lian contain tube micro porous filter device 3RS3 carry out it is micro-
Filter, filter core are made of its matched import filter membrane, 0.2 μm of aperture.
Comparative example 3: test water enters small-sized streaming electrochemical reactor with the flow velocity of 100mL/min, passes through T-type before
For charge pipe with the sodium chloride solution of the flow velocity addition 10mmol/L of 1mL/min, working electrode is boron-doped diamond electrode, auxiliary electricity
Extremely platinum electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, apply voltage and are
1.5V。
Comparative example 4: test water enters Philip UV-17W ultraviolet sterilizer, ultraviolet irradiation with the flow velocity of 100mL/min
Dosage is 55mJcm-2, time of contact 4s；Water outlet enters Jiang great Lian and contains tube micro porous filter device 3RS3 progress micro-filtration, filter core
It is made of its matched import filter membrane, 0.2 μm of aperture.
Comparative example 5: test water enters Philip UV-17W ultraviolet sterilizer, ultraviolet irradiation with the flow velocity of 100mL/min
Dosage is 55mJcm-2, time of contact 4s；The chlorine of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min
Change sodium solution；Water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum electricity
Pole, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V.
Comparative example 6: test water with the flow velocity of 100mL/min enter Jiang great Lian contain tube micro porous filter device 3RS3 carry out it is micro-
Filter, filter core are made of its matched import filter membrane, 0.2 μm of aperture；Water outlet enters Philip UV-17W ultraviolet sterilizer, ultraviolet spoke
It is 55mJcm according to dosage-2, time of contact 4s.
Comparative example 7: test water with the flow velocity of 100mL/min enter Jiang great Lian contain tube micro porous filter device 3RS3 carry out it is micro-
Filter, filter core are made of its matched import filter membrane, 0.2 μm of aperture；Water outlet is added by T-type charge pipe with the flow velocity of 1mL/min
The sodium chloride solution of 10mmol/L；Water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, auxiliary
Helping electrode is platinum electrode, and reference electrode is saturated calomel electrode, provides power supply using CHI604D electrochemical analyser, applies electricity
Pressure is 1.5V.
Comparative example 8: test water enters small-sized streaming electrochemical reactor with the flow velocity of 100mL/min, passes through T-type before
For charge pipe with the sodium chloride solution of the flow velocity addition 10mmol/L of 1mL/min, working electrode is boron-doped diamond electrode, auxiliary electricity
Extremely platinum electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, apply voltage and are
1.5V；Water outlet enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose 55mJcm-2, time of contact 4s.
Comparative example 9: test water enters small-sized streaming electrochemical reactor with the flow velocity of 100mL/min, passes through T-type before
For charge pipe with the sodium chloride solution of the flow velocity addition 10mmol/L of 1mL/min, working electrode is boron-doped diamond electrode, auxiliary electricity
Extremely platinum electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, apply voltage and are
1.5V；Water outlet enters Jiang great Lian and contains tube micro porous filter device 3RS3 progress micro-filtration, and filter core is made of its matched import filter membrane, hole
0.2 μm of diameter.
Comparative example 10: test water enters Philip UV-17W ultraviolet sterilizer, ultraviolet irradiation with the flow velocity of 100mL/min
Dosage is 55mJcm-2, time of contact 4s；The chlorine of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min
Change sodium solution；Water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum electricity
Pole, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V；Water outlet
Tube micro porous filter device 3RS3 is contained into Jiang great Lian and carries out micro-filtration, and filter core is made of its matched import filter membrane, 0.2 μm of aperture.
Comparative example 11: test water enters Jiang great Lian with the flow velocity of 100mL/min and contains tube micro porous filter device 3RS3 progress
Micro-filtration, filter core are made of its matched import filter membrane, 0.2 μm of aperture；Water outlet enters Philip UV-17W ultraviolet sterilizer, ultraviolet
Irradiation dose is 55mJcm-2, time of contact 4s；Water outlet adds 10mmol/L by T-type charge pipe with the flow velocity of 1mL/min
Sodium chloride solution；Water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is
Platinum electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V.
Comparative example 12: test water enters Jiang great Lian with the flow velocity of 100mL/min and contains tube micro porous filter device 3RS3 progress
Micro-filtration, filter core are made of its matched import filter membrane, 0.2 μm of aperture；Water outlet is added by T-type charge pipe with the flow velocity of 1mL/min
Add the sodium chloride solution of 10mmol/L；Water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode,
Auxiliary electrode is platinum electrode, and reference electrode is saturated calomel electrode, provides power supply using CHI604D electrochemical analyser, applies
Voltage is 1.5V；Water outlet enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose 55mJcm-2, time of contact is
4s。
Comparative example 13: test water enters small-sized streaming electrochemical reactor with the flow velocity of 100mL/min, passes through T before
Type charge pipe is boron-doped diamond electrode, auxiliary with the sodium chloride solution of the flow velocity addition 10mmol/L of 1mL/min, working electrode
Electrode is platinum electrode, and reference electrode is saturated calomel electrode, provides power supply using CHI604D electrochemical analyser, applies voltage
For 1.5V；Water outlet enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose 55mJcm-2, time of contact 4s；Out
Water enters Jiang great Lian and contains tube micro porous filter device 3RS3 progress micro-filtration, and filter core is made of its matched import filter membrane, 0.2 μ of aperture
m。
Comparative example 14: test water enters small-sized streaming electrochemical reactor with the flow velocity of 100mL/min, passes through T before
Type charge pipe is boron-doped diamond electrode, auxiliary with the sodium chloride solution of the flow velocity addition 10mmol/L of 1mL/min, working electrode
Electrode is platinum electrode, and reference electrode is saturated calomel electrode, provides power supply using CHI604D electrochemical analyser, applies voltage
For 1.5V；Water outlet enters Jiang great Lian and contains tube micro porous filter device 3RS3 progress micro-filtration, and filter core is made of its matched import filter membrane,
0.2 μm of aperture；Water outlet enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose 55mJcm-2, time of contact is
4s。
Experimental result: using colony counting method and SYBR Green qPCR method respectively to before sterilizing and purifying and through each work
The resistance bacterium of typical Tetracyclines, sulfamido, erythromycin series in water outlet after skill sterilizing and purifying and corresponding resistant gene tet
(A), tet (O), sul (1), sul (2), erm (B), erm (F) are measured, and calculate removal rate, as a result as shown in Figure 2.It is logical
Cross comparison as it can be seen that the disinfection and purification system that proposes of this patent to the removal effect of resistance bacterium and resistant gene in source water most
It is good.
Comparative example 15: ultraviolet ray disinfector 2 will be obtained in the present invention, millipore filter 4 and electrochemical reactor 7 are at other
Test is compared in the case that condition is constant, the result of comparison is as shown in the table: (wherein I expression first step connection is this
Device, II indicates the device of second step connection, and III indicates the device of third step connection ,~indicate not connected device)
It was found from comparative example 15；Optimal operating procedure is in technical solution of the present invention: ultraviolet ray disinfector → micropore
This process of filter → electrochemical reactor.
Generate this phenomenon theoretically analyze the reason is that: the present invention in ultraviolet sterilizer effect be remove and kill
Most of bacterium including antibiotic-resistant bacteria, while intracellular resistant gene is removed to a certain extent；Micropore mistake
The effect of filter is the resistance bacterium of removal dead bacterial body and a small amount of work；The effect of electrochemical reactor is to completely remove water
In deliquescent extracellular resistant gene and a small amount of resistant gene intracellular；Finally by water source resistance bacterium and resistant gene it is complete
Removal, has ensured water supply security.
If having lacked ultraviolet sterilizer, tolerant bacteria is not inactivated effectively, and intracellular resistant gene also obtains
Less than effective removal, and a large amount of living cells directly passes through millipore filter and be easy to cause blocking, and the effect of filtering declines,
The poor removal effect of resistance bacterium, removal efficiency of the resistant gene intracellular in electrochemical reactor is low, eventually leads to sterilizing and purifying
It is ineffective.
If having lacked millipore filter, tolerant bacteria further cannot be removed efficiently, a large amount of dead thin
Thallus enters electrochemical reactor, and the load that will lead to electrochemical treatments increases, and the efficiency for removing resistant gene significantly reduces, electricity
The energy consumption of chemistry also increases, and it is ineffective to eventually lead to sterilizing and purifying.
If having lacked electrochemical reactor, original in the water of source and resistance bacterium is released after ultraviolet kill
The extracellular resistant gene of dissolubility will be unable to be effectively removed, it is ineffective to eventually lead to sterilizing and purifying.
Further, since electrochemical reactor be mainly used for original in the water of removal source and killed bacterial body release it is molten
The extracellular resistant gene of solution property and a small amount of resistant gene intracellular reduce energy consumption to improve the treatment effeciency of electrochemistry, it is necessary to will
Electrochemical reactor is placed on final step；Ultraviolet sterilizer and millipore filter can remove resistance bacterium to a certain extent, and two
Person, which is used in combination, is greatly improved the removal effect of resistance bacterium, at the same in view of after ultraviolet-sterilization part bacterium have showing for dark reactivation
As, and a large amount of viable bacteria bodies is avoided to cause blocking to influence filter effect by millipore filter, reduce resistance on microstrainer to the greatest extent
The secondary pollution of bacterium, so ultraviolet sterilizer must be placed on before millipore filter.
Embodiment 5: it using water outlet of the Nanjing waterworks after coagulation, precipitating, filtering as test water, is testing
Under the scale of room, the disinfection and purification system proposed through this patent is handled, it may be assumed that
1) test water enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose point with the flow velocity of 100mL/min
It is not set as 40,45,55,65,70mJcm-2, time of contact 4s；
2) water outlet enters Jiang great Lian Sheng tube micro porous filter device 3RS3 progress micro-filtration, and filter core is its matched import filter membrane system
At 0.2 μm of aperture；
3) sodium chloride solution of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min；
4) water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum
Electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V.
Experimental result: using colony counting method and SYBR Green qPCR method respectively to before sterilizing and purifying and myristylpicolinum bromide
The resistance bacterium of typical Tetracyclines, sulfamido, erythromycin series in water outlet after change and corresponding resistant gene tet (A), tet
(O), sul (1), sul (2), erm (B), erm (F) are measured, and calculate removal rate, as a result as shown in Figure 3.It can by comparison
See, UV irradiation dose is 45~65mJcm in the disinfection and purification system that this patent proposes-2When source water in resistance bacterium and
The removal effect of resistant gene is best.
Embodiment 6: it using water outlet of the Nanjing waterworks after coagulation, precipitating, filtering as test water, is testing
Under the scale of room, the disinfection and purification system proposed through this patent is handled, it may be assumed that
1) test water enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose point with the flow velocity of 100mL/min
55mJcm is not set as it-2, time of contact 4s；
2) water outlet enters Jiang great Lian Sheng tube micro porous filter device 3RS3 progress micro-filtration, and filter core is its matched import filter membrane system
At aperture is respectively 0.1,0.15,0.2,0.25,0.3 μm；
3) sodium chloride solution of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min；
4) water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum
Electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, application voltage is 1.5V.
Experimental result: using colony counting method and SYBR Green qPCR method respectively to before sterilizing and purifying and myristylpicolinum bromide
The resistance bacterium of typical Tetracyclines, sulfamido, erythromycin series in water outlet after change and corresponding resistant gene tet (A), tet
(O), sul (1), sul (2), erm (B), erm (F) are measured, and calculate removal rate, as a result as shown in Figure 4.It can by comparison
See, this patent propose disinfection and purification system in micro porous filtration aperture be 0.15~0.25 μm when source water in resistance bacterium and
The removal effect of resistant gene is best.
Embodiment 7: it using water outlet of the Nanjing waterworks after coagulation, precipitating, filtering as test water, is testing
Under the scale of room, the disinfection and purification system proposed through this patent is handled, it may be assumed that
1) test water enters Philip UV-17W ultraviolet sterilizer, UV irradiation dose point with the flow velocity of 100mL/min
55mJcm is not set as it-2, time of contact 4s；
2) water outlet enters Jiang great Lian Sheng tube micro porous filter device 3RS3 progress micro-filtration, and filter core is its matched import filter membrane system
At aperture is respectively 0.2 μm；
3) sodium chloride solution of 10mmol/L is added in water outlet by T-type charge pipe with the flow velocity of 1mL/min；
4) water outlet enters small-sized streaming electrochemical reactor, and working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum
Electrode, reference electrode are saturated calomel electrode, provide power supply using CHI604D electrochemical analyser, apply voltage and are respectively
0.75、1、1.25、1.5、1.75V。
Experimental result: using colony counting method and SYBR Green qPCR method respectively to before sterilizing and purifying and myristylpicolinum bromide
The resistance bacterium of typical Tetracyclines, sulfamido, erythromycin series in water outlet after change and corresponding resistant gene tet (A), tet
(O), sul (1), sul (2), erm (B), erm (F) are measured, and calculate removal rate, as a result as shown in Figure 4.It can by comparison
See, the resistance of electrochemical reactor applied when voltage is 1~1.5V in source water in the disinfection and purification system that this patent proposes
The removal effect of bacterium and resistant gene is best.
It should be noted that above-mentioned is only presently preferred embodiments of the present invention, protection model not for the purpose of limiting the invention
It encloses, any combination or equivalents made on the basis of the above embodiments all belong to the scope of protection of the present invention.
Claims (7)
1. a kind of drinking water disinfection purification system for handling antibiotic resistance bacterium and resistant gene, which is characterized in that described is net
Change system includes ultraviolet ray disinfector, millipore filter and electrochemical reactor；The ultraviolet ray disinfector is equipped with and drinks
Water water inlet, ultraviolet ray disinfector are sequentially connected millipore filter and electrochemical reactor by pipeline, and the electrochemistry is anti-
Device is answered to be equipped with drinking water water outlet, the connecting line between the millipore filter and electrochemical reactor is equipped with T-type
Charge pipe, the T-type charge pipe are equipped with second level inlet.
2. the drinking water disinfection purification system of processing antibiotic antibiotic bacteria and antibiosis gene as described in claim 1, feature
It is, the inlet and outlet on the ultraviolet ray disinfector are arranged on sustained height, the electrochemical reactor
Inlet and outlet setting in sustained height, the water inlet setting of the millipore filter is in bottom, water outlet setting
At top.
3. the drinking water disinfection purification system of processing antibiotic resistance bacterium and resistant gene as described in claim 1, feature
It is, auxiliary electrode, reference electrode and working electrode, the auxiliary electrode and reference is equipped in the electrochemical reactor
The top of electrochemical reactor is arranged in electrode, and the bottom of electrochemical reactor is arranged in the working electrode.
4. a kind of drinking water disinfection purification process for handling antibiotic resistance bacterium and resistant gene, which is characterized in that the work
Skill includes the following steps:
1) it is passed through ultraviolet ray disinfector by pretreated drinking water with the flow velocity of 100mL/min to be handled, wherein ultraviolet
Irradiation dose is 45~65mJ/cm2, time of contact 4s；
2) drinking water for obtaining step 1) is passed through in millipore filter and handles, wherein the filter hole in millipore filter
Diameter is 0.15~0.25 μm；
3) sodium chloride solution is added into the drinking water for obtaining step 2), so that concentration of the sodium chloride in drinking water is maintained at
0.1~0.2mmol/L；
4) drinking water for obtaining step 3) is passed through electrochemical reactor and handles, and the operating voltage of electrochemical reactor is 1
~1.5V；
5) be by step 4) treated drinking water safety drinking water.
5. the drinking water disinfection purification process of processing antibiotic resistance bacterium and resistant gene as described in claim 1, feature
It is, the pretreated drinking water in the step 1) is the drinking water after coagulation, precipitating, filtration treatment.
6. the drinking water disinfection purification process of processing antibiotic resistance bacterium and resistant gene as described in claim 1, feature
It is, in the step 4), working electrode is boron-doped diamond electrode, and auxiliary electrode is platinum electrode, and reference electrode is saturation
Calomel electrode.
7. the drinking water disinfection purification process of processing antibiotic resistance bacterium and resistant gene as described in claim 1, feature
It is, in the step 2), used microfiltration membranes and filter core need to be disposed through drying, calcination, could put into secondary use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811292577.9A CN109095679B (en) | 2018-11-01 | 2018-11-01 | Drinking water disinfection and purification system for treating antibiotic resistance bacteria and resistance genes and process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811292577.9A CN109095679B (en) | 2018-11-01 | 2018-11-01 | Drinking water disinfection and purification system for treating antibiotic resistance bacteria and resistance genes and process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109095679A true CN109095679A (en) | 2018-12-28 |
| CN109095679B CN109095679B (en) | 2024-06-28 |
Family
ID=64870084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811292577.9A Active CN109095679B (en) | 2018-11-01 | 2018-11-01 | Drinking water disinfection and purification system for treating antibiotic resistance bacteria and resistance genes and process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109095679B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111620493A (en) * | 2020-07-03 | 2020-09-04 | 中国农业科学院农业环境与可持续发展研究所 | Method and special equipment for removing antibiotic resistant bacteria and resistant genes in sewage |
| CN113087244A (en) * | 2021-04-14 | 2021-07-09 | 广州普德生物科技有限公司 | System for purifying antibiotics in drinking water of water plant |
| CN113620391A (en) * | 2021-07-15 | 2021-11-09 | 暨南大学 | Method and device for removing antibiotic-resistant bacteria and/or antibiotic-resistant genes in water |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100846075B1 (en) * | 2008-02-12 | 2008-07-15 | (주) 시온텍 | Hybrid ultraviolet sterilizer |
| US20140124440A1 (en) * | 2012-03-29 | 2014-05-08 | Nanjing University | Method for removing genes encoding antibiotic resistance |
| CN107986517A (en) * | 2017-11-17 | 2018-05-04 | 浙江海洋大学 | The processing method of resistant gene in a kind of marine culture wastewater |
| CN108358386A (en) * | 2018-03-02 | 2018-08-03 | 成都知联汇科技有限公司 | A kind for the treatment of process of antibiotic pharmaceutical wastewater |
| CN209210551U (en) * | 2018-11-01 | 2019-08-06 | 南京林业大学 | A kind of drinking water disinfection purification system handling antibiotic resistance bacterium and resistant gene |
-
2018
- 2018-11-01 CN CN201811292577.9A patent/CN109095679B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100846075B1 (en) * | 2008-02-12 | 2008-07-15 | (주) 시온텍 | Hybrid ultraviolet sterilizer |
| US20140124440A1 (en) * | 2012-03-29 | 2014-05-08 | Nanjing University | Method for removing genes encoding antibiotic resistance |
| CN107986517A (en) * | 2017-11-17 | 2018-05-04 | 浙江海洋大学 | The processing method of resistant gene in a kind of marine culture wastewater |
| CN108358386A (en) * | 2018-03-02 | 2018-08-03 | 成都知联汇科技有限公司 | A kind for the treatment of process of antibiotic pharmaceutical wastewater |
| CN209210551U (en) * | 2018-11-01 | 2019-08-06 | 南京林业大学 | A kind of drinking water disinfection purification system handling antibiotic resistance bacterium and resistant gene |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111620493A (en) * | 2020-07-03 | 2020-09-04 | 中国农业科学院农业环境与可持续发展研究所 | Method and special equipment for removing antibiotic resistant bacteria and resistant genes in sewage |
| CN113087244A (en) * | 2021-04-14 | 2021-07-09 | 广州普德生物科技有限公司 | System for purifying antibiotics in drinking water of water plant |
| CN113087244B (en) * | 2021-04-14 | 2021-12-28 | 湖南城头山矿泉水科研开发有限公司 | System for purifying antibiotics in drinking water of water plant |
| CN113620391A (en) * | 2021-07-15 | 2021-11-09 | 暨南大学 | Method and device for removing antibiotic-resistant bacteria and/or antibiotic-resistant genes in water |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109095679B (en) | 2024-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209210551U (en) | A kind of drinking water disinfection purification system handling antibiotic resistance bacterium and resistant gene | |
| CN109095679A (en) | A kind of drinking water disinfection purification system and its technique handling antibiotic resistance bacterium and resistant gene | |
| WO2011120250A1 (en) | Method and system for ship ballast water treatment | |
| CN211570348U (en) | Direct drinking water purifying and sterilizing device | |
| JP4906572B2 (en) | Method of inactivating Legionella spp. In circulating water tank | |
| CN107162123A (en) | Filtering sterilization method and device and application in a kind of electrode | |
| CN105540761A (en) | Water disinfection device and water disinfection method performed through water disinfection device | |
| Gholami et al. | The electrochemical removal of bacteria from drinking water | |
| CN107055913B (en) | Chlorine-free swimming pool disinfection system and method | |
| CN103539298A (en) | Water treatment purifying device with time control switch as well as circuit structure thereof | |
| CN202099149U (en) | Small-sized medical sewage treater | |
| CN107158423A (en) | Dental medical apparatus chlorination equipment and method | |
| CN215798962U (en) | Ultrapure water device for laboratory | |
| CN203513404U (en) | Automatic swimming pool silver ion sterilization system | |
| CN108083540A (en) | A kind of desk type water purification | |
| CN207581551U (en) | A kind of drinking water purification device | |
| Vaze et al. | Air and water sterilization using non-thermal plasma | |
| CN209853785U (en) | Ultraviolet sterilizer for purifying drinking water | |
| CN112645495A (en) | Disinfection equipment suitable for small-size medical institution | |
| CN104528996A (en) | High-efficiency sterilization device and method and swimming pool water treatment circulating system with sterilization device | |
| CN207632640U (en) | Tap water supply purifier based on ionization sterilization | |
| CN208008626U (en) | The water cleaning systems of desk type water purification | |
| CN206799353U (en) | Without chlorine sterilizing swimming pool system | |
| CN206521354U (en) | Urban community's direct drinking water treatment facilities | |
| CN212532603U (en) | Swimming pool water softening installation that disinfects |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |