CN109092778A - Rotary membrane washing device for immunoblotting test - Google Patents
Rotary membrane washing device for immunoblotting test Download PDFInfo
- Publication number
- CN109092778A CN109092778A CN201810721953.5A CN201810721953A CN109092778A CN 109092778 A CN109092778 A CN 109092778A CN 201810721953 A CN201810721953 A CN 201810721953A CN 109092778 A CN109092778 A CN 109092778A
- Authority
- CN
- China
- Prior art keywords
- locating rack
- washing
- rotary
- locating piece
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/048—Overflow-type cleaning, e.g. tanks in which the liquid flows over the tank in which the articles are placed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/041—Cleaning travelling work
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/044—Cleaning involving contact with liquid using agitated containers in which the liquid and articles or material are placed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Abstract
The invention discloses a rotary membrane washing device for an immunoblotting test, which comprises an outer barrel with an opening at the top end, wherein the side wall of the outer barrel is provided with at least two through holes positioned at different heights, a membrane washing liquid conveying branch pipe penetrates through the through holes, the output end of the membrane washing liquid conveying branch pipe is positioned inside the outer barrel, the input end of the membrane washing liquid conveying branch pipe is positioned outside the outer barrel, the input end of the membrane washing liquid conveying branch pipe is communicated with a conveying pipe, the conveying pipe is connected with one end of a circulating pipe through a connecting hose, the other; the top end opening part of outer bucket is equipped with the top cap, and the bottom axle center position of top cap is equipped with the locating rack of vertical setting, and the top of locating rack is connected with the top cap through the bearing, is equipped with two at least washing membrane wares on the locating rack, and the central point that washes the membrane ware puts and fixes on the locating rack, fixes being equipped with driving motor on the support, fixes being equipped with down the locating piece on driving motor's the output shaft, and the bottom mounting of locating rack is equipped with the locating piece. The invention has the advantage of uniform film washing.
Description
Technical field
The present invention relates to field of bioexperiment technology, are filled more particularly to a kind of rotary western blot test with film is washed
It sets.
Background technique
Western blot test (immunoblotting) i.e. Western Blot.It is molecular biology, biochemistry and exempts from
Common a kind of experimental method in epidemic disease science of heredity.The basic principle is that by specific antibody to the processed cell of gel electrophoresis
Or biological tissue samples are coloured.It is thin what is analyzed by the position and color depth acquisition specific protein of analysis coloring
Born of the same parents or tissue in expression information.
In the prior art, in Western Blot experiment, there is " washing a film " step, the major way for washing film at present is
Band is placed directly within and fills the washing in film ware of TBST, is then placed on to shake on shaking table and washes film, often will cause band heap in this way
Product is together, it is difficult to abundant cleansing bars band.It is a kind of can effectively solve problem above wash film device up for further studying
And exploitation.
Summary of the invention
In view of the technical drawbacks of the prior art, it is an object of the present invention to provide a kind of rotary immunoblottings to try
It tests with washing film device.
The technical solution adopted to achieve the purpose of the present invention is:
A kind of rotary western blot test of the invention is with washing film device, the outer barrel including top end opening, said tub
Side wall be equipped at least two be located at different height through-holes, film washing liquid delivery branch pipe pass through the through-hole and output end
Positioned at the inside of said tub, input terminal is located at the outside of said tub, and the input terminal is connected with delivery pipe, institute
It states delivery pipe and is connected by connection hose with one end of circulation pipe, the other end of the circulation pipe is located at bottom in said tub
It holds, is serially connected with circulating pump on the circulation pipe；
Top cover is equipped at the top end opening of said tub, the bottom end shaft core position of the top cover is equipped with the positioning being vertically arranged
The top of frame, the locating rack is connected by bearing with top cover, and the locating rack is equipped at least two and washes film ware, described to wash
The center of film ware is fixed on locating rack, and the number for washing film ware is identical as the number of the through-hole；
The bottom end of said tub is fixed with bracket, and driving motor is fixed on the bracket, the driving motor
Lower locating piece is fixed on output shaft, the bottom end of the locating rack is fixed with locating piece, the lower locating piece, upper positioning
Block matches, to realize that output shaft and locating rack rotate synchronously.
Preferably, support outer rim is equipped at the top end opening of said tub.
Preferably, the lower locating piece is square block, and the upper locating piece is cylindrical structure, and bottom end is equipped with and institute
State the groove that lower locating piece matches.
Preferably, the top of the top cover is fixed with handle.
Preferably, the film ware of washing is circular configuration.
Preferably, it is described wash film ware set there are three~six.
Preferably, the locating rack is equipped with external screw thread, the top dead center position of the upper locating piece be equipped with it is described outer
The threaded hole that screw thread matches, the center for washing film ware are equipped with through-hole, and the diameter of the through-hole is greater than the locating rack
Outer diameter, the locating rack is equipped with multiple positioning bolts engaged, and the outer diameter of the positioning bolt is greater than the through-hole
Diameter.
Preferably, the inside edge for washing film ware and outer ledge position are equipped with flanging.
Compared with prior art, the beneficial effects of the present invention are:
1, revolving to wash membrane structure, so that washing, film is more uniform, improves the reliability of experiment.
2, film washing liquid recycles, energy saving.
3, film ware is washed convenient for picking and placing, and flexibility in use is high.
4, reasonable in design, space is saved, low manufacture cost is easy to promote and utilize.
Detailed description of the invention
Fig. 1 show structural schematic diagram of the invention.
Fig. 2 show the bottom view of locating piece.
Fig. 3 is shown
Wherein: 1- outer barrel, 2- top cover, 3- handle, 4- driving motor, 5- output shaft, locating piece under 6-, the upper locating piece of 7-,
8- locating rack, 9- wash film ware, 10- bearing, 11- delivery pipe, 12- film washing liquid delivery branch pipe, 13- connection hose, 14- circulation pipe,
15- circulating pump, 16- support outer rim.
Specific embodiment
The present invention is described in further detail below in conjunction with the drawings and specific embodiments.It should be appreciated that described herein
Specific embodiment be only used to explain the present invention, be not intended to limit the present invention.
Embodiment 1
As shown in Figs. 1-2, a kind of rotary western blot test of the invention is outer including top end opening with film device is washed
Bucket 1, the side wall of said tub 1 are equipped at least two through-holes for being located at different height, and film washing liquid delivery branch pipe 12 passes through described
Through-hole and output end be located at the inside of said tub 1, input terminal is located at the outside of said tub 1, and the input terminal
It is connected with delivery pipe 11, the delivery pipe 11 is connected by connection hose 13 with one end of circulation pipe 14, the circulation
The other end of pipe 14 is located at bottom end in said tub 1, is serially connected with circulating pump 15 on the circulation pipe 14；
It is equipped with top cover 2 at the top end opening of said tub 1, the bottom end shaft core position of the top cover 2, which is equipped with, to be vertically arranged
The top of locating rack 8, the locating rack 8 is connected by bearing 10 with top cover 2, and the locating rack 8 is equipped at least two and washes
Film ware 9, the center for washing film ware 9 are fixed on locating rack 8, and wash the number of film ware 9 and the number of the through-hole
It is identical；
The bottom end of said tub 1 is fixed with bracket, and driving motor 4, the driving motor 4 are fixed on the bracket
Output shaft 5 on be fixed with lower locating piece 6, the bottom end of the locating rack 8 is fixed with locating piece 7, the lower locating piece
6, upper locating piece 7 matches, to realize that output shaft 5 and locating rack 8 rotate synchronously.
Working method:
When using the present apparatus, delivery pipe 11 is first pulled outward, and film washing liquid is added into outer barrel 1 and liquid level is no more than branch
Top cover 2 and its film ware 9 of washing that its bottom end is arranged in are taken out in frame top, are placed on band tiling wash in film ware 9 respectively, so
The lid of top cover 2 is closed into the aperture position on 1 top of outer barrel afterwards, at this moment, lower locating piece 6, upper locating piece 7 are meshed, and push inwardly
Delivery pipe 11, at this moment, the output end of film washing liquid delivery branch pipe 12 are located at the surface for washing film ware 9, start circulating pump 15, wash
Film liquid is entered by the output end of film washing liquid delivery branch pipe 12 washes in film ware, starts driving motor 4, output shaft 5 drives locating rack 8
Rotation, at the same time, washes 9 synchronous rotary of film ware, and film washing liquid is constantly entered and washed in film ware, and the part of spilling enters outer
Bottom end in bucket 1, it is energy saving to recycle.
It is preferred that being equipped with support outer rim 16 at the top end opening of said tub 1, the stabilization to realize top cover 2 is fixed
Position.
It is preferred that the lower locating piece 6 is square block, the upper locating piece 7 is cylindrical structure, and bottom end
Equipped with the groove to match with the lower locating piece 6.In this way, lower locating piece 6, upper locating piece 7 can be passed through when output shaft 5 rotates
Locating rack 8 is driven to rotate steadily.
It is preferred that the top of the top cover 2 is fixed with handle 3, convenient for lifting top cover 2 and its being arranged at its bottom
Film ware 9 is washed at end.
It is preferred that the film ware 9 of washing is circular configuration.
It is preferred that it is described wash film ware 9 set there are three~six.
Embodiment 2
The present embodiment is further improved on the basis of embodiment 1.
As shown in figure 3, the locating rack 8 is equipped with external screw thread, the top dead center position of the upper locating piece 7 is equipped with and institute
The threaded hole 16 that external screw thread matches is stated, the center for washing film ware 9 is equipped with through-hole 17, and the diameter of the through-hole 17 is greater than
The outer diameter of the locating rack 8, the locating rack 8 are equipped with multiple positioning bolts 18 engaged, the positioning bolt 18
Outer diameter is greater than the diameter of the through-hole 17.
It is preferred that the inside edge for washing film ware 9 and outer ledge position are equipped with flanging 19.
It is serially connected on locating rack 8 in use, film ware 9 will be washed, and the fixed position for washing film ware 9 of the positioning bolt 18 by screwing in
It sets, so washes film ware 9 convenient for assembly or disassembly, washed in film ware 9 convenient for band to be placed into, while being also convenient for it from washing film ware
It is taken out in 9.
The above is only a preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of rotary western blot test is with washing film device, which is characterized in that the outer barrel including top end opening, said tub
Side wall be equipped at least two be located at different height through-holes, film washing liquid delivery branch pipe pass through the through-hole and output end
Positioned at the inside of said tub, input terminal is located at the outside of said tub, and the input terminal is connected with delivery pipe, institute
It states delivery pipe and is connected by connection hose with one end of circulation pipe, the other end of the circulation pipe is located at bottom in said tub
It holds, is serially connected with circulating pump on the circulation pipe；
Top cover is equipped at the top end opening of said tub, the bottom end shaft core position of the top cover is equipped with the locating rack being vertically arranged,
The top of the locating rack is connected by bearing with top cover, and the locating rack is equipped at least two and washes film ware, described to wash film
The center of ware is fixed on locating rack, and the number for washing film ware is identical as the number of the through-hole；
The bottom end of said tub is fixed with bracket, and driving motor, the output of the driving motor are fixed on the bracket
Lower locating piece is fixed on axis, the bottom end of the locating rack is fixed with locating piece, the lower locating piece, upper locating piece phase
Matching, to realize that output shaft and locating rack rotate synchronously.
2. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that said tub
Support outer rim is equipped at top end opening.
3. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that the lower positioning
Block is square block, and the upper locating piece is cylindrical structure, and bottom end is equipped with the groove to match with the lower locating piece.
4. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that the top cover
Top is fixed with handle.
5. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that described to wash film ware
For circular configuration.
6. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that described to wash film ware
There are three if~six.
7. a kind of rotary western blot test as described in claim 1 is with washing film device, which is characterized in that the locating rack
It is equipped with external screw thread, the top dead center position of the upper locating piece is equipped with the threaded hole to match with the external screw thread, described to wash
The center of film ware is equipped with through-hole, and the diameter of the through-hole is greater than the outer diameter of the locating rack, and the locating rack is equipped with more
A positioning bolt engaged, the outer diameter of the positioning bolt are greater than the diameter of the through-hole.
8. a kind of rotary western blot test as claimed in claim 7 is with washing film device, which is characterized in that described to wash film ware
Inside edge and outer ledge position be equipped with flanging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810721953.5A CN109092778A (en) | 2018-07-04 | 2018-07-04 | Rotary membrane washing device for immunoblotting test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810721953.5A CN109092778A (en) | 2018-07-04 | 2018-07-04 | Rotary membrane washing device for immunoblotting test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109092778A true CN109092778A (en) | 2018-12-28 |
Family
ID=64845593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810721953.5A Pending CN109092778A (en) | 2018-07-04 | 2018-07-04 | Rotary membrane washing device for immunoblotting test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109092778A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5360741A (en) * | 1992-09-29 | 1994-11-01 | Triangle Biomedical Sciences, Inc. | DNA hybridization incubator |
| CN106607358A (en) * | 2016-12-27 | 2017-05-03 | 郑州吉音动漫科技有限公司 | Multi-groove type medical cleaning and drying device |
| CN206373123U (en) * | 2016-12-04 | 2017-08-04 | 缙云县阪喜达包装有限公司 | A kind of multilayer cleaning device |
| CN107823655A (en) * | 2017-12-13 | 2018-03-23 | 王周滢 | A kind of Infectious Disease's Medical appliance decontaminating apparatus and sterilization method |
-
2018
- 2018-07-04 CN CN201810721953.5A patent/CN109092778A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5360741A (en) * | 1992-09-29 | 1994-11-01 | Triangle Biomedical Sciences, Inc. | DNA hybridization incubator |
| CN206373123U (en) * | 2016-12-04 | 2017-08-04 | 缙云县阪喜达包装有限公司 | A kind of multilayer cleaning device |
| CN106607358A (en) * | 2016-12-27 | 2017-05-03 | 郑州吉音动漫科技有限公司 | Multi-groove type medical cleaning and drying device |
| CN107823655A (en) * | 2017-12-13 | 2018-03-23 | 王周滢 | A kind of Infectious Disease's Medical appliance decontaminating apparatus and sterilization method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181228 |