CN109090116A - A kind of innoxious composition and preparation method thereof for killing mosquito larvae - Google Patents
A kind of innoxious composition and preparation method thereof for killing mosquito larvae Download PDFInfo
- Publication number
- CN109090116A CN109090116A CN201811062787.9A CN201811062787A CN109090116A CN 109090116 A CN109090116 A CN 109090116A CN 201811062787 A CN201811062787 A CN 201811062787A CN 109090116 A CN109090116 A CN 109090116A
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- China
- Prior art keywords
- composition
- carrier
- hormone analog
- innoxious
- composition according
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
Abstract
A kind of innoxious composition and preparation method thereof for killing mosquito larvae, its composition includes Bacillus thuringiensis var.israelensis de Barjac, corpus allatum hormone analog, emulsifier, carrier and optional vegetable oil；Carrier is quartz sand or slag particle；Corpus allatum hormone analog is Nylar and/or methoprene；Preparation method include emulsification, sprinkling mixing, stirring, covering and etc..Composition made of the present invention is the compounding of pure natural soil bacteria product and artificial synthesized corpus allatum hormone analog；There is quick-acting, residual effect, non-target organism safety and the beneficial effect without drug resistance in terms of effect；The pollution-free noresidue in terms of environmental protection has high vegetation penetration power.After composition finished product vacuum sealed package made of the present invention, it can save at room temperature 2 years.
Description
Technical field
The present invention relates to field of public health, specially a kind of innoxious composition for killing mosquito larvae and its preparation side
Method.
Background technique
Mosquito is the main vector of a variety of human and animal's diseases including malaria, yellow fever and dengue fever.It is annual super
The disease that 1,000,000 people die of killing propagation is crossed, several hundred million people are even more to undergo pain and with the disease by killing propagation.
For dengue fever both without specific drug or without vaccine.The sole mode for controlling the disease at present is that disease is propagated in control
The mosquito of disease, i.e. Aedes aegypti (Aedes aegypti) and aedes albopictus (Aedes albppictus).Yellow fever is not controlled
Treatment method, but have vaccine；However vaccine is expensive, can not obtain other areas to protect the world.Currently without obtainable
Pharmaceutical admixtures guarantee 100% protection for malaria, and other than preventing bite by mosquitos, the prevention of infection is needed by guidance
Use anti-malaria medicaments.Anti-Malarial is actually not to prevent the disease, but only work in blood flow by inhibiting red
Parasitic animal and plant in cell develops to inhibit clinical symptoms.
In China, what mosquito control relied on for a long time is non-selective organochlorine, organic phosphorus, carbamates
Close object and pyrethroid；There is non-target organism toxicity, environmental pollution, target biomolecule resistants etc. for multiple medications above-mentioned
A variety of inevitable drawbacks, these drawbacks make foregoing pharmaceutical effect more and more weaker, and exactly mosquito and mosquito matchmaker's disease are sudden and violent in recent years
Send out a popular big factor.
Summary of the invention
The first purpose of the invention is to provide a kind of innoxious compositions for killing mosquito larvae larva.Of the invention
Two purposes are to provide the preparation method of above-mentioned composition.
The first purpose of this invention can be achieved by the following technical programs:
A kind of innoxious composition for killing mosquito larvae, which is characterized in that calculating by percentage to the quality includes with the following group
Point:
Preferably, component further includes vegetable oil, and content is 2.0-5.0% mass percent.
Preferably, the carrier is 30-40 mesh, the quartz sand of 400-600 micron diameter.
Preferably, the carrier is slag particle.
Preferably, the emulsifier is Triton-100.
Preferably, the corpus allatum hormone analog is Nylar and/or methoprene；When corpus allatum hormone analog
Its content is 0.05-0.5% mass percent when for Nylar, and when corpus allatum hormone analog is methoprene, content is
0.25-5% mass percent.
Second object of the present invention can be achieved by the following technical programs:
A kind of preparation method of the above-mentioned innoxious composition for killing mosquito larvae, comprising the following steps:
1) it takes the corpus allatum hormone analog of above-mentioned mass percent and emulsifier to mix to complete emulsification, obtains A；
2) A is sprayed on carrier, and is constantly stirred carrier during sprinkling；
3) Bacillus thuringiensis var.israelensis de Barjac bacterium powder is continuously added in whipping process；
4) it stirs to the surface attachments covering on carrier uniformly, obtains finished product.
Bacillus thuringiensis var.israelensis de Barjac used in the present invention is insecticidal active ingredient, and finished product potency is in 100-
Between 400/mg, bacterium powder, potency substantially 7000/mg are used in the production process.Bacillus thuringiensis var.israelensis de Barjac is
A kind of mosquito children midfield toxic protein, can rapidly kill mosquito larvae.
Corpus allatum hormone analog used in the present invention includes Nylar and/or methoprene, is another desinsection
Effective component has growth regulating effect to mosquito larvae, can upset the normal processes of mosquito larvae pupa emergence.
Emulsifier used in the present invention is Triton X-100 (Triton X-100), is a kind of non-ionic
Surfactant.Emulsion can be formed as setting up bridge between water phase in oil.Remaining emulsifier can also make according to demand
With.
Carrier used in the present invention is 30-40 mesh (400-600 micron diameter) pure quartz sand, it is desirable that surface is without dust etc.
Impurity attachment, has the advantages that high specific gravity and high-specific surface area.The pure quartz sand of 30-40 mesh can be substituted with slag particle,
The density and granular size of slag particle are needed with the close of the pure quartz sand of 30-40 mesh.
It, can be suitably in composition if necessary to improve the content of the Bacillus thuringiensis var.israelensis de Barjac in composition through a step
Middle addition vegetable oil, as natural adhesive.
Compared with prior art, composition made of the present invention is that pure natural soil bacteria product and artificial synthesized insect protect
The compounding of young hormone analogs；There is quick-acting, residual effect, non-target organism safety and the beneficial effect without drug resistance in terms of effect.
In addition, the manufactured pollution-free noresidue of composition, has high vegetation penetration power, uniform fold is easy production, and performance is steady
Fixed, storage transport is easy to use, environmentally friendly.After composition finished product vacuum sealed package made of the present invention, in room temperature item
It can be saved under part 2 years.
Specific embodiment
Specific embodiment is provided below, and the invention will be further described, so that those skilled in the art can preferably manage
The solution present invention can be simultaneously practiced, but illustrated embodiment is not as a limitation of the invention.
The production method of embodiment:
1) component needed for weighing as shown in table 1；
2) corpus allatum hormone analog and emulsifier are mixed agitation to mix to complete emulsification, obtains A；
3) A is sprayed on carrier, and so continuous that mix carrier Stirring during sprinkling；
4) while being stirred, Bacillus thuringiensis var.israelensis de Barjac bacterium powder is slowly added, as having plant in component
Oily then plus vegetable oil；
5) continue to stir, until carrier surface attachment covering uniformly, obtain finished product.
6) finished product is packed into vacuum damp-proofing bag (20 kilograms) in large-scale application of pesticides or water-soluble cellulose vacuum bag
(10 grams/bag) are used for processing small water.
The innoxious killing mosquito larvae composition components content of table 1
Quick-acting desinsection tests
Tested mosquito kind: laboratory Aedes aegypti population (Aedes aegypti).
Age: third-instar larvae
Dosage range: 0.225-2.25ppm；
Product 200mg in Example 1, mixes with 20ml water, then dilutes 10 times and 100 times, obtains product design and is
0.1% and 0.01% sample.
It is put into mosquito larvae (wriggler) in the sample at 25 DEG C.
| Concentration (million/) | 0.225 | 0.75 | 1.125 | 2.25 | Control |
| Total amount of liquid (milliliter) | 100 | 100 | 100 | 100 | 100 |
| Required milliliter 0.01% | 225 | 750 | 1125 | 0 | |
| Required milliliter 0.1% | 225 | 0 | |||
| Repeat 1 | 25 | 25 | 25 | 25 | 25 |
| It is dead to repeat 1/ | 1 | 11 | 19 | 25 | 0 |
| Repeat 2 | 25 | 25 | 25 | 25 | 25 |
| It is dead to repeat 2/ | 2 | 18 | 14 | 23 | 0 |
| Repeat 3 | 25 | 25 | 25 | 25 | 25 |
| It is dead to repeat 3/ | 0 | 15 | 18 | 20 | 0 |
| Larva amounts to | 75 | 75 | 75 | 75 | 75 |
| Death amounts to | 3 | 44 | 51 | 68 | 0 |
Read result: 24 hours after application (dead and dying larva)；
Analysis: 50% lethasl concentration and 90% lethasl concentration are calculated with other in relation to parameter with concentration-probability of death.
As a result such as following table
Residual effect test
Tested mosquito kind: laboratory Aedes aegypti population (Aedes aegypti).
Age: four-age larva
Dosage range: 0.10-5.00ppb；
Product 160mg in Example 1, mixes with 20ml water, then dilutes 10 times and 100 times, obtains product design and is
0.0001%, 0.00001% and 0.000001% sample.
It is put into mosquito larvae (wriggler) in the sample at 25 DEG C, until all larvae pupations, sprouts wings or dead, take 5-7
It.
Read result: 5-7 days after application, to individual without existence, reading dead larva, dead pupa and not exclusively sprout wings at
Mosquito.
Analysis: 50% lethasl concentration and 90% lethasl concentration are calculated with other in relation to parameter with concentration-probability of death.
As a result such as following table
| LC50=1.194ppb (95% fiducial limit=0.894-1.687ppb) |
| LC90=9.885ppb (95% fiducial limit=5.800-21.356ppb) |
| H=0.39 |
| Slope=1.396+/- 0.144 |
The above embodiment of the present invention is not limiting the scope of the present invention, and embodiments of the present invention are not limited to
This, all this kind above content according to the present invention is not departing from this according to the ordinary technical knowledge and customary means of this field
Modification, replacement or change under the premise of inventing above-mentioned basic fundamental thought, to other diversified forms that above structure of the present invention is made
More, it should all fall within the scope and spirit of the invention.
Claims (7)
1. a kind of innoxious composition for killing mosquito larvae, which is characterized in that calculating by percentage to the quality includes following components:
2. composition according to claim 1, it is characterised in that: further include component botanical oil, content 2.0-5.0%
Mass percent.
3. composition according to claim 1, it is characterised in that: the carrier is 30-40 mesh quartz sand.
4. composition according to claim 1, it is characterised in that: the carrier is 30-40 mesh slag particle.
5. composition according to claim 1, it is characterised in that: the emulsifier is Triton X-100.
6. composition according to claim 1, it is characterised in that: the corpus allatum hormone analog be Nylar and/
Or methoprene；When corpus allatum hormone analog is Nylar, its content is 0.05-0.5% mass percent, when insect protects
Content is 0.25-5% mass percent when young hormone analogs are methoprene.
7. the preparation method of the composition of any innoxious killing mosquito larvae of claim 1-6, which is characterized in that packet
Include following steps:
1) it takes the corpus allatum hormone analog of above-mentioned mass percent and emulsifier to mix to complete emulsification, obtains A；
2) A is sprayed on carrier, and is constantly stirred carrier during sprinkling；
3) Bacillus thuringiensis var.israelensis de Barjac bacterium powder is continuously added in whipping process；
4) it stirs to the surface attachments covering on carrier uniformly, obtains finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811062787.9A CN109090116A (en) | 2018-09-12 | 2018-09-12 | A kind of innoxious composition and preparation method thereof for killing mosquito larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811062787.9A CN109090116A (en) | 2018-09-12 | 2018-09-12 | A kind of innoxious composition and preparation method thereof for killing mosquito larvae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109090116A true CN109090116A (en) | 2018-12-28 |
Family
ID=64865926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811062787.9A Pending CN109090116A (en) | 2018-09-12 | 2018-09-12 | A kind of innoxious composition and preparation method thereof for killing mosquito larvae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109090116A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110946150A (en) * | 2019-12-27 | 2020-04-03 | 武汉市拜乐卫生科技有限公司 | Compound mosquito killing oil suspension and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491080A (en) * | 2001-02-16 | 2004-04-21 | �Ϻ���ͨ��ѧ | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| US7196116B1 (en) * | 2004-04-20 | 2007-03-27 | Wellmark International | Solid pesticide compositions with extended stability |
| CN103547159A (en) * | 2011-04-07 | 2014-01-29 | 发育研究院 | Composition for anti-mosquito use |
-
2018
- 2018-09-12 CN CN201811062787.9A patent/CN109090116A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491080A (en) * | 2001-02-16 | 2004-04-21 | �Ϻ���ͨ��ѧ | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| US7196116B1 (en) * | 2004-04-20 | 2007-03-27 | Wellmark International | Solid pesticide compositions with extended stability |
| CN103547159A (en) * | 2011-04-07 | 2014-01-29 | 发育研究院 | Composition for anti-mosquito use |
Non-Patent Citations (2)
| Title |
|---|
| 王以燕: "WHO推荐防制重要媒介生物的卫生杀虫用农药及其应用", 《中华卫生杀虫药械》 * |
| 陈勇奎等编著: "《农药使用技术》", 31 July 1995, 上海科学普及出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110946150A (en) * | 2019-12-27 | 2020-04-03 | 武汉市拜乐卫生科技有限公司 | Compound mosquito killing oil suspension and preparation method thereof |
| CN110946150B (en) * | 2019-12-27 | 2021-08-27 | 武汉市拜乐卫生科技有限公司 | Compound mosquito killing oil suspension and preparation method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181228 |
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| RJ01 | Rejection of invention patent application after publication |