CN109060991A - A kind of content assaying method of food vitamins B6 - Google Patents
A kind of content assaying method of food vitamins B6 Download PDFInfo
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- CN109060991A CN109060991A CN201811010919.3A CN201811010919A CN109060991A CN 109060991 A CN109060991 A CN 109060991A CN 201811010919 A CN201811010919 A CN 201811010919A CN 109060991 A CN109060991 A CN 109060991A
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- vitamin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The invention discloses the content assaying methods of food vitamins B6 a kind of, it include: the preparation of the preparation of standard solution stock solution, the preparation of standard solution and test solution, precision measures standard solution and each 10 μ l of test solution injects liquid chromatograph, record chromatogram, by external standard method with the content of calculated by peak area food vitamins B6, detection accuracy is high.
Description
Technical field
The present invention relates to vitamin content measuring methods, and in particular to a kind of assay side of food vitamins B6
Method.
Background technique
Vitamin B6 (Vitamin B6) is also known as pyridoxine comprising pyridoxol, pyridoxal and pyridoxamine, in vivo with phosphorus
The form of acid esters exists, and is a kind of water soluble vitamin, and the constituent of the internal certain coenzyme of vitamin B6 behaviour participates in a variety of
Metabolic response especially has substantial connection with amino acid metabolism；The vomiting of pregnancy clinically is prevented and treated using Vitamin B6 preparation and is put
Penetrate disease vomiting.
In process of production, content is quality to vitamin B6, by carrying out content detection to crude product, control matter
Amount.
Traditional detection method is typically all the content for passing through other substances in detection crude product, to detect containing for vitamin B6
Amount, detection cycle length, complex steps, the factor of influence precision is more in detecting step, is unfavorable for industrial production to vitamin
The detection of B6 content.
Summary of the invention
The present invention is directed at least solve one of the technical problems existing in the prior art.For this purpose, the present invention provides a kind of food
The content assaying method of vitamin B6 in product, detection accuracy are high.
Above-mentioned purpose is achieved by the following technical solution:
A kind of content assaying method of food vitamins B6, comprising:
The preparation of standard solution stock solution: taking vitamin B6 reference substance 25mg, accurately weighed, sets in 50ml volumetric flask,
Phased soln and it is diluted to scale with flowing, shaken up, faced with now matching；
The preparation of standard solution: taking standard solution stock solution 2ml, set in 10ml volumetric flask, simultaneously with flowing phased soln
It is diluted to scale, is shaken up, is faced with now matching；
The preparation of test solution: taking this product, finely ground, takes fine powder, accurately weighed, sets in 100ml volumetric flask, adds mobile phase
80ml, ultrasound make to dissolve for 30 minutes, scale are diluted to after cooling, as test solution；
Precision measures standard solution and each 10 μ l of test solution injects liquid chromatograph, chromatogram is recorded, by external standard
Method is with the content of calculated by peak area food vitamins B6.
In some embodiments, the specific preparation steps of the mobile phase are as follows:
Sodium pentanesulfonate 0.4g is taken, adds water 1000ml to dissolve, with glacial acetic acid tune pH value to 3.3 to prepare 0.04% pentane sulphur
Acid sodium solution；
Taking A is 0.04% pentanesulfonic acid sodium solution, and B is methanol, is mixed by A:B=86:14, and 0.45 μm of filter membrane, ultrasound are crossed
Degassing is to prepare mobile phase.
In some embodiments, purity >=99% of vitamin B6 reference substance.
In some embodiments, the content of food vitamins B6 is indicated with the every hectogram of milligram, is carried out as follows
It calculates:
In formula:
X is the content of vitamin B6 in test sample, and unit is the every hectogram of milligram (mg/100g)；
A1 is the peak area of vitamin B6 in test sample；
M2 is the quality of vitamin B6 reference substance, and unit is milligram；
C2 is the content of vitamin B6 reference substance；
V1 is the extension rate of test sample；
A2 is the peak area of vitamin B6 reference substance；
M1 is the quality of test sample, and unit is gram；
V2 is vitamin B6 reference substance extension rate；
100 be conversion coefficient, and the conversion factor of mg/100g is converted by mg/g；
Calculated result is indicated with the arithmetic mean of instantaneous value of the measurement result independent twice obtained under the conditions of repeatability.
In some embodiments, the absolute difference of the measurement result independent twice obtained under the conditions of repeatability, which is less than, to be calculated
The 10% of art average value.
The technical scheme provided by this disclosed embodiment can include the following benefits:
Using measuring method of the invention, detection cycle is short, step is simple, detection accuracy is high, conducive to tieing up in food
The detection of raw element B6 content.
It should be understood that above general description and following detailed description be only it is exemplary and explanatory, not
The disclosure can be limited.
Detailed description of the invention
Fig. 1 shows the flow diagram of measuring method in embodiment.
Specific embodiment
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Embodiment one: as shown in Figure 1, the present embodiment is the content assaying method of food vitamins B6 a kind of, comprising:
The preparation of standard solution stock solution: taking vitamin B6 reference substance 25mg, accurately weighed, sets in 50ml volumetric flask,
Phased soln and it is diluted to scale with flowing, shaken up, faced with now matching；
The preparation of standard solution: taking standard solution stock solution 2ml, set in 10ml volumetric flask, simultaneously with flowing phased soln
It is diluted to scale, is shaken up, is faced with now matching；
The preparation of test solution: taking this product, finely ground, takes fine powder, accurately weighed, sets in 100ml volumetric flask, adds mobile phase
80ml, ultrasound make to dissolve for 30 minutes, scale are diluted to after cooling, as test solution；
Precision measures standard solution and each 10 μ l of test solution injects liquid chromatograph, chromatogram is recorded, by external standard
Method is with the content of calculated by peak area food vitamins B6.
The measuring method of the present embodiment, detection cycle is short, step is simple, detection accuracy is high, is conducive to give birth to dimension in food
The detection of plain B6 content.
Following expansion statement is made with regard to the content assaying method of food vitamins B6 provided by the present embodiment below:
1, reagent and material
Unless otherwise specified this method agents useful for same is that analysis is pure, water is level-one water as defined in GB/T 6682.
1.1 reagent
1.1.1 sodium pentanesulfonate (C5H11NaO3S).
1.1.2 glacial acetic acid (CH3COOH).
1.1.3 methanol (CH3OH): chromatographically pure.
1.2 preparation of reagents
1.2.1 0.04% pentanesulfonic acid sodium solution: taking sodium pentanesulfonate 0.4g, adds water 1000ml to dissolve, with glacial acetic acid tune
PH value is to 3.3.
1.2.2 mobile phase: A:0.04% pentanesulfonic acid sodium solution: B: methanol.By A: B=86: 14 mixing, 0.45 μm of filter is crossed
Film, ultrasonic degassing.
1.3 standard items
The vitamin B6 reference substance of Chinese food drug assay research institute purchase, or through national authentication and authorize standard substance
The vitamin B6 reference substance of certificate, purity >=99%.
1.4 standard solution stock solutions
1.4.1 the preparation of standard solution stock solution: taking vitamin B6 reference substance 25mg, accurately weighed, sets 50ml capacity
It in bottle, is flowed with 1.2.2 and phased soln and is diluted to scale, shaken up, faced with now matching.
1.5 standard solution
1.5.1 the preparation of standard solution: 1.4 standard solution stock solution 2ml are taken, sets in 10ml volumetric flask, uses 1.2.2
Flowing phased soln is simultaneously diluted to scale, shakes up, and faces with now matching.
2, instrument and equipment
2.1 liquid chromatographs: it is furnished with UV detector.
2.2pH meter: precision 0.01.
2.3 balance；Sensibility reciprocal is 0.1mg.
2.4 ultrasonic cleaner.
2.5 filter membranes: 0.45 μm of organic phase film.
3, analytical procedure
Entire detection process carries out under the conditions of being protected from light as far as possible.
The preparation of 3.1 test solutions
This product is taken, it is finely ground, it takes fine powder (being approximately equivalent to vitamin B6 10mg), it is accurately weighed, it sets in 100ml volumetric flask, adds
Mobile phase 80ml, ultrasound make to dissolve for 30 minutes, scale are diluted to after cooling, as test solution.
4, instrument reference conditions
4.1 chromatographic columns: C18 column, column length 250mm, internal diameter 4.6mm, 5 μm of partial size, or the chromatographic column of equal performance.
4.2 detector: UV detector.
4.3 flow velocitys: 1.0mL/min.
4.4 Detection wavelengths: 285nm.
4.5 column temperatures: 25 DEG C.
4.6 sample volumes: 10 μ L.
5, sample introduction is analyzed
5.1 accurate measurement standard solutions and each 10 μ l of test solution inject liquid chromatograph, chromatogram are recorded, by outer
Mark method is with calculated by peak area.
6, the statement of result is analyzed
Vitamin B6 content is indicated in sample with the every hectogram of milligram, is calculated as follows:
In formula:
X is the content of vitamin B6 in test sample, and unit is the every hectogram of milligram (mg/100g)；
A1 is the peak area of vitamin B6 in test sample；
M2 is the quality of vitamin B6 reference substance, and unit is milligram；
C2 is the content of vitamin B6 reference substance；
V1 is the extension rate of test sample；
A2 is the peak area of vitamin B6 reference substance；
M1 is the quality of test sample, and unit is gram；
V2 is vitamin B6 reference substance extension rate；
100 be conversion coefficient, and the conversion factor of mg/100g is converted by mg/g；
Calculated result is indicated with the arithmetic mean of instantaneous value of the measurement result independent twice obtained under the conditions of repeatability.7, precision
The absolute difference of the measurement result independent twice obtained under the conditions of repeatability is less than the 10% of arithmetic mean of instantaneous value.
By using the measuring method of the present embodiment, detection cycle can be effectively shortened, and its step is simple, detection essence
Degree is high, conducive to the detection to food vitamins B6 content.
Above-described is only some embodiments of the present invention.For those of ordinary skill in the art, not
Under the premise of being detached from the invention design, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.
Claims (5)
1. a kind of content assaying method of food vitamins B6 characterized by comprising
The preparation of standard solution stock solution: taking vitamin B6 reference substance 25mg, accurately weighed, sets in 50ml volumetric flask, with stream
Dynamic phased soln is simultaneously diluted to scale, shakes up, and faces with now matching；
The preparation of standard solution: taking standard solution stock solution 2ml, set in 10ml volumetric flask, with flowing phased soln and dilutes
It to scale, shakes up, faces with now matching；
The preparation of test solution: taking this product, finely ground, takes fine powder, accurately weighed, sets in 100ml volumetric flask, adds mobile phase
80ml, ultrasound make to dissolve for 30 minutes, scale are diluted to after cooling, as test solution；
Precision measures standard solution and each 10 μ l of test solution injects liquid chromatograph, records chromatogram, by external standard method with
The content of calculated by peak area food vitamins B6.
2. a kind of content assaying method of food vitamins B6 according to claim 1, which is characterized in that the mobile phase
Specific preparation steps are as follows:
Sodium pentanesulfonate 0.4g is taken, adds water 1000ml to dissolve, with glacial acetic acid tune pH value to 3.3 to prepare 0.04% sodium pentanesulfonate
Solution；
Taking A is 0.04% pentanesulfonic acid sodium solution, and B is methanol, is mixed by A:B=86:14, and 0.45 μm of filter membrane, ultrasonic degassing are crossed
To prepare mobile phase.
3. a kind of content assaying method of food vitamins B6 according to claim 2, which is characterized in that vitamin B6 pair
According to purity >=99% of product.
4. a kind of content assaying method of food vitamins B6 according to claim 3, which is characterized in that tie up life in food
The content of plain B6 is indicated with the every hectogram of milligram, is calculated as follows:
In formula:
X is the content of vitamin B6 in test sample, and unit is the every hectogram of milligram (mg/100g)；
A1 is the peak area of vitamin B6 in test sample；
M2 is the quality of vitamin B6 reference substance, and unit is milligram；
C2 is the content of vitamin B6 reference substance；
V1 is the extension rate of test sample；
A2 is the peak area of vitamin B6 reference substance；
M1 is the quality of test sample, and unit is gram；
V2 is vitamin B6 reference substance extension rate；
100 be conversion coefficient, and the conversion factor of mg/100g is converted by mg/g；
Calculated result is indicated with the arithmetic mean of instantaneous value of the measurement result independent twice obtained under the conditions of repeatability.
5. a kind of content assaying method of food vitamins B6 according to claim 4, which is characterized in that in repeated item
The absolute difference of the measurement result independent twice obtained under part is less than the 10% of arithmetic mean of instantaneous value.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114280198A (en) * | 2021-12-30 | 2022-04-05 | 中国大冢制药有限公司 | Vitamin B6Detection method and application of related substances thereof |
| CN114324636A (en) * | 2021-12-21 | 2022-04-12 | 新发药业有限公司 | Method for determining vitamin B6 and related impurities thereof by HPLC method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114324636A (en) * | 2021-12-21 | 2022-04-12 | 新发药业有限公司 | Method for determining vitamin B6 and related impurities thereof by HPLC method |
| CN114324636B (en) * | 2021-12-21 | 2024-03-15 | 新发药业有限公司 | Method for measuring vitamin B6 and related impurities thereof by HPLC (high Performance liquid chromatography) |
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