CN108645925B - Method for detecting related substances in cinnamyl octane related products through high performance liquid chromatography - Google Patents
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Abstract
A method for detecting related substances in a guimeixin related product by high performance liquid chromatography applied to the field of drug detection adopts a chromatographic column with octadecylsilane chemically bonded silica as a filler; acetonitrile and potassium dihydrogen phosphate buffer solution as gradient elution mobile phase: the method can track and trace impurities generated in the synthesis process of the cinnamyl acetate, has the advantages of good specificity, high sensitivity, durability, detection limit and the like, can meet the requirements compared with the prior art, can be widely applied to the field of pharmaceutical analysis, and provides important basis for product quality control and process optimization.
Description
Technical Field
The invention relates to a method for detecting related substances in a guimeixin related product through high performance liquid chromatography in the field of pharmaceutical analysis.
Background
The cinnamyl methylcin, also known as indomethacin, indomethacin and cinnamic acid, is an anti-inflammatory analgesic, and can reduce the synthesis of prostaglandin by inhibiting cyclooxygenase to produce analgesic effect. By inhibiting chemotaxis of leukocytes and release of lysosomes, inflammatory symptoms are inhibited. The inhibition of hypothalamic thermoregulation central prostaglandin synthesis and release causes peripheral vasodilation and sweating, which increases heat dissipation and produces antipyretic effect. The Guimeixin is applicable to acute and chronic rheumatic arthritis, rheumatoid arthritis, ankylosing spondylitis and other inflammatory pains, and has obvious curative effect on renal colic diseases. The cinnamyl caprylic acid has the characteristics of strong anti-inflammatory activity, obvious anti-edema effect, low toxicity and safe taking, and is widely applied clinically.
According to the regulation of national drug standard WS-10001- (HD-0272) -2002, the current inspection method of the related substances of the cinnamyl acetate adopts thin-layer chromatography, and the method has poor specificity, low sensitivity and poor repeatability, can only carry out limited detection on the related substances, and cannot quantify the related substances. And the method for detecting the quantification of related substances in the products related to the cinnamyl metacin (the initial raw material and the intermediate for synthesizing the cinnamyl metacin and the finished product of the cinnamyl metacin) is not reported in related documents. Therefore, the development of a simple and efficient method for detecting related substances in the products related to the cinnamyl octane is a new problem to be solved urgently.
Disclosure of Invention
The invention aims to provide a method for detecting related substances in a cinnamyl octane related product by high performance liquid chromatography. The method is simultaneously suitable for detecting related substances of the initial raw materials and the intermediate for synthesizing the cinnamyl octyl and the finished cinnamyl octyl product, has good specificity and high sensitivity, and can carry out quantitative detection on impurities. The method adopts gradient elution, and adjusts the pH value of the mobile phase B potassium dihydrogen phosphate buffer solution, so that the initial raw material, the intermediate and the finished product of the cinnamyl caprylic have reasonable retention time, can well separate the components, is favorable for tracking and tracing the generated impurities in the synthesis process of the cinnamyl caprylic, and provides important basis for product quality control and process optimization.
The purpose of the invention is realized as follows: a method for detecting related substances in a cinnamyl octane related product by high performance liquid chromatography comprises the following steps:
(1) chromatographic conditions are as follows:
a chromatographic column: chromatographic column with octadecylsilane chemically bonded silica as filler;
mobile phase: the mobile phase A is acetonitrile;
mobile phase B, potassium dihydrogen phosphate buffer solution:
the mobile phase adopts a gradient elution mode;
(2) preparation of a test solution:
accurately weighing a proper amount of sample, adding acetonitrile to dissolve and dilute the sample to be used as a test solution;
(3) preparation of control solution:
precisely measuring a proper amount of a test solution, and diluting the test solution with a mobile phase to be used as a reference solution;
(4) the determination method comprises the following steps:
injecting the reference solution into a liquid chromatograph, and adjusting the detection sensitivity to make the peak height of the main component chromatographic peak be 20% of the full-scale range; precisely measuring the test solution and the reference solution, injecting into a liquid chromatograph, recording the map, measuring by using a main component self-reference method, wherein the peak area of a single impurity is not more than 1.5 times of the main peak area of the reference solution, the sum of the peak areas of all impurities is not more than 2 times of the main peak area of the reference solution, and calculating the content of the single impurity and the total impurity;
a method for detecting related substances in a cinnamyl octane related product by high performance liquid chromatography comprises the following steps:
(1) chromatographic conditions are as follows:
a chromatographic column: chromatographic column with octadecylsilane chemically bonded silica as filler;
mobile phase: the mobile phase A is acetonitrile;
mobile phase B, potassium dihydrogen phosphate buffer solution:
the mobile phase adopts a gradient elution mode;
(2) preparation of a test solution:
accurately weighing a proper amount of sample, adding acetonitrile to dissolve and dilute the sample to be used as a test solution;
(3) the determination method comprises the following steps:
injecting the sample solution into a liquid chromatograph, recording a color spectrum, measuring by using an area normalization method, and calculating the content of single impurities and total impurities;
the mobile phase B is a potassium dihydrogen phosphate buffer aqueous solution, and the pH value is adjusted to 4.0 by a potassium hydroxide aqueous solution; the mobile phase B is 0.01mol/L potassium dihydrogen phosphate buffer aqueous solution, and the pH value is adjusted to 4.0 by 20 percent potassium hydroxide aqueous solution; in the step (2), the sample is a finished product of the cinnamyl acetate; the sample volume of the control solution is 10-100 mul, and the preferred sample volume of the control solution is 20 mul; the preparation method of the control solution comprises the following steps: precisely measuring 1ml of a test solution, placing the test solution in a 100ml measuring flask, diluting the test solution to a scale with a mobile phase, and shaking up; in the step (2), the sample is a raw material for synthesizing the cinnamyl acetate or an intermediate for synthesizing the cinnamyl acetate; the raw material for synthesizing the cinnamyl methyl ether comprises p-anisidine, and the intermediate for synthesizing the cinnamyl methyl ether comprises sodium p-methyl phenyl hydrazine sulfonate and cinnamyl hydrazine; the specific method of gradient elution is as follows: the content of the mobile phase A is 10 to 50 percent and the content of the mobile phase B is 90 to 50 percent in 0 minute; for 6 minutes, the content of the mobile phase A is 10-50 percent, and the content of the mobile phase B is 90-50 percent; for 26 minutes, the content of the mobile phase A is 50-90 percent, and the content of the mobile phase B is 50-10 percent; for 30 minutes, the content of the mobile phase A is 50-90 percent, and the content of the mobile phase B is 50-10 percent; for 40 minutes, the content of the mobile phase A is 10-50 percent, and the content of the mobile phase B is 90-50 percent; for 50 minutes, the content of the mobile phase A is 10-50 percent, and the content of the mobile phase B is 90-50 percent; the preferred specific method of gradient elution is: 0 minute, mobile phase a is 30%, mobile phase B is 70%; for 6 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent; 26 minutes, 70% of mobile phase A and 30% of mobile phase B; 30 minutes, 70% of mobile phase A and 30% of mobile phase B; for 40 minutes, the content of the mobile phase A is 30 percent, and the content of the mobile phase B is 70 percent; for 50 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent; the detector of the detection method is an ultraviolet detector, the detection wavelength of the ultraviolet detector is 291nm, the flow rate of the mobile phase is 0.8ml/min-1.2ml/min, the flow rate of the preferred mobile phase is 1.0ml/min, the column temperature is 25-45 ℃, the preferred column temperature is 35 ℃, the sample volume of the sample solution is 10-100 mul, and the preferred sample volume of the sample solution is 20 mul; the preparation method of the test sample solution comprises precisely weighing a proper amount of the test sample, adding acetonitrile to dissolve and quantitatively diluting to prepare a solution containing 0.2mg of acetonitrile in each 1ml of the solution as the test sample solution; in the method, the calculation method of the single impurities and the total impurities comprises the following steps:
the method is simultaneously suitable for the detection of the related substances of a starting material para-anisidine for synthesizing the cinnamyl methacin, and the related substances of main intermediates of sodium p-toluenesulfonate, cinnamyl hydrazine and a cinnamyl methacin finished product, and can track and trace impurities generated in the synthesis process of the cinnamyl methacin.
Drawings
FIG. 1 is a high performance liquid chromatogram for detecting related substances in a finished product of cinnamyl acetate
FIG. 2 is a high performance liquid chromatogram for detecting substances related to intermediate cinnamyl hydrazine
FIG. 3 is a high performance liquid chromatogram for detecting related substances of intermediate sodium p-methyl phenylhydrazine sulfonate
FIG. 4 is a high performance liquid chromatogram for detecting related substances of p-anisidine as a starting material for synthesizing cinnamyl acetate
Detailed Description
The following examples will help to understand the present invention, but they are only for illustrative purposes and the present invention is not limited to these contents.
Example one
Selection of mobile phase
Two mobile phase systems of acetic acid water solution-acetonitrile, phosphate buffer solution-acetonitrile are selected. When an acetic acid aqueous solution-acetonitrile system is used, all components cannot be completely separated; the phosphate buffer solution-acetonitrile system has small concentration of selected buffer salt, ensures that the retention time of chromatographic peaks has better repeatability, and has less damage to chromatographic columns, symmetrical peak shapes and good separation effect. Therefore, phosphate buffer-acetonitrile system was chosen as the mobile phase.
Example two
Establishment of methodology
1. Specificity test
1.1 separation of the main starting materials, intermediates, degradation products and major peak of cinnamyl
Precisely measuring 20 mul of a solution of cinnamyl octyl, p-anisidine, sodium p-methyl phenyl hydrazine sulfonate, cinnamyl hydrazine and acid and alkali degradation products, injecting the solution into a liquid chromatograph, recording a chromatogram, and displaying that the separation degrees of all the components meet the requirements by the spectrogram.
Table 1: main starting raw material, intermediate, degradation product and main peak-off time of synthetic cinnamyl octyl
1.2 acid, base, thermal destruction test
Acid destruction test solution: accurately weighing 10mg of cinnamyl octyl (batch number: 20150815), placing the cinnamyl octyl (batch number: 20150815) into a 50ml measuring flask, adding 2.0ml of 1.0mol/L hydrochloric acid solution, standing for 2h, adding 2.0ml of 1.0mol/L sodium hydroxide solution for neutralization, adding acetonitrile for dilution to scale, shaking up, and using the mixture as acid to destroy a sample solution.
Alkali destruction test solution: accurately weighing 10mg of cinnamyl octyl (batch number: 20150815), placing in a 50ml measuring flask, adding 2.0ml of 0.1mol/L sodium hydroxide solution, standing for 5min, adding 2.0ml of 0.1mol/L hydrochloric acid solution for neutralization, adding acetonitrile for dilution to scale, shaking up, and using as an alkali to destroy a sample solution.
Thermally destroying the test solution: placing the cinnamyl acetate (batch number: 20150815) in a 105 ℃ oven, taking out after 5h, precisely measuring 10mg, placing in a 50ml measuring flask, adding acetonitrile to dilute to a scale, shaking up, and using as a thermal destruction test solution.
And precisely measuring 20 mu l of each test sample solution, injecting the solution into a high performance liquid chromatograph, and recording a chromatogram, wherein the result shows that the separation degree of each degradation product and the main peak of the cinnamyl octyl in the cinnamyl metacin sample meets the analysis and determination requirements of the high performance liquid chromatography.
Table 2: results of acid, base, high temperature (thermal) destruction tests (normalized by area)
The result shows that the cinnamyl acetate is unstable under the condition of acid and alkali destruction, and is particularly unstable to alkali; under high temperature destruction conditions, impurities are slightly increased; in addition, the data can judge that the material balance before and after the destruction, and the method can be used for detecting substances related to the cinnamyl octane.
2. Durability test
2.1 stability Studies of solutions
Weighing 10mg of the definite guimeixin precisely, placing the definite guimeixin in a 50ml volumetric flask, adding acetonitrile to dissolve and dilute the definite guimeixin to a scale, taking the definite guimeixin as a test solution, placing the definite guimeixin at room temperature for 0 to 8 hours, precisely measuring 20 mu l of the definite guimeixin, injecting the definite guimeixin into a liquid chromatograph, and measuring the peak area of each impurity, wherein the result shows that the test solution is stable within 8 hours.
Table 3: stability test results of the solution
2.2 investigation of different columns
Two different brands of C18 chromatographic columns are selected to measure related substances, the measurement results of the maximum single impurity and the maximum total impurity are basically consistent, and the brands of the chromatographic columns have no influence on the detection results.
Table 4: investigation results of different chromatography columns
3. Detection limit
Taking a proper amount of the cinnamectin, precisely weighing, adding acetonitrile to dissolve and dilute the cinnamectin into a series of concentrations, precisely measuring 20 mu l of the cinnamectin, injecting the cinnamectin into a high performance liquid chromatograph, recording a chromatogram, and determining the sample injection concentration as the detection limit of the cinnamectin when S/N is approximately equal to 3, wherein the sample injection concentration is 0.04 mu g/ml. The result shows that the method for determining the substances related to the cinnamyl methacin by adopting the high performance liquid chromatography is accurate and feasible.
EXAMPLE III
And (4) measuring related substances of the finished product of the cinnamyl hydroxide.
1. Chromatographic conditions are as follows:
a chromatographic column: waters
C18(4.6 × 250mm,5 μm), detection wavelength 291nm, flow rate 1.0ml/min, column temperature 35 ℃, mobile phase A acetonitrile, mobile phase B potassium dihydrogen phosphate buffer solution 0.01mol/L (adjusted to pH4.0 with 20% potassium hydroxide solution);
the mobile phase adopts a gradient elution mode, and the gradient elution is carried out according to the following table:
table 5: gradient elution chart
2. Preparation of relevant solution and mobile phase:
2.1 preparation of the relevant solutions
Preparation of 2.1.120% Potassium hydroxide solution
20g of potassium hydroxide is weighed, dissolved in water and diluted to 100 ml.
Preparation of 2.1.20.01 mol/L potassium dihydrogen phosphate buffer solution
1.3609g of monopotassium phosphate was weighed, dissolved in water and diluted to 1000ml, and the pH was adjusted to 4.0 with 20% potassium hydroxide.
2.1.3 preparation of test solutions
Precisely weighing appropriate amount of the digoxigenin, adding acetonitrile to dissolve, and quantitatively diluting to obtain a solution containing about 0.2mg of the digoxigenin per 1ml, wherein the solution is used as a test solution.
2.1.4 preparation of control solutions
Precisely measuring 1ml of the test solution, placing the test solution in a 100ml measuring flask, diluting the test solution to a scale with a mobile phase, and shaking the test solution uniformly to obtain a control solution.
2.2 preparation of the Mobile phase
Mixing the 0.01mol/L potassium dihydrogen phosphate buffer solution and acetonitrile according to the volume ratio of gradient elution to be used as a mobile phase.
3. The method for measuring the substances related to the cinnamyl acetate comprises the following steps:
injecting 20 μ l of the control solution into a liquid chromatograph, and adjusting the detection sensitivity to make the peak height of the main component chromatographic peak about 20% of the full scale; and precisely measuring 20 mul of the test solution and 20 mul of the reference solution, injecting into a liquid chromatograph, recording a chromatogram, and measuring by using a main component self-reference method, wherein the peak area of a single impurity is not more than 1.5 times of the main peak area of the reference solution, and the sum of the peak areas of all impurities is not more than 2 times of the main peak area of the reference solution.
4. Calculating the content of individual impurities and total impurities
Calculating the formula:
and (3) calculating the result:
example four
And (3) measuring related substances in the raw material p-anisidine for synthesizing the cinnamyl octane.
1. Chromatographic conditions are as follows:
a chromatographic column: waters
C18(4.6×250mm,5 μm); the detection wavelength is 291 nm; the flow rate is 1.0 ml/min; column temperature: 35 ℃; mobile phase A acetonitrile, mobile phase B0.01 mol/L potassium dihydrogen phosphate buffer solution (adjusted to pH4.0 with 20% potassium hydroxide solution);
the mobile phase adopts a gradient elution mode, and the gradient elution is carried out according to the following table:
table 6: gradient elution chart
2. Preparation of relevant solution and mobile phase:
2.1 preparation of the relevant solutions
Preparation of 2.1.120% Potassium hydroxide solution
20g of potassium hydroxide is weighed, dissolved in water and diluted to 100 ml.
Preparation of 2.1.20.01 mol/L potassium dihydrogen phosphate buffer solution
1.3609g of monopotassium phosphate was weighed, dissolved in water and diluted to 1000ml, and the pH was adjusted to 4.0 with 20% potassium hydroxide.
2.1.3 preparation of test solutions
Precisely weighing a proper amount of p-anisidine, adding acetonitrile to dissolve, and quantitatively diluting to obtain a solution containing about 0.2mg per 1ml, wherein the solution is used as a test solution.
2.2 preparation of the Mobile phase
Mixing the 0.01mol/L potassium dihydrogen phosphate buffer solution and acetonitrile according to the volume ratio of gradient elution to be used as a mobile phase.
3. The method for measuring the related substances of the para-anisidine comprises the following steps:
and (3) taking 20 mu l of test solution, injecting into a liquid chromatograph, recording a color spectrum, and measuring by an area normalization method.
4. The result of the detection
0.06 percent of single impurity
100-99.88% of total impurities and 0.12%
EXAMPLE five
And (3) measuring related substances in the sodium p-toluenesulfonate intermediate for synthesizing the cinnamyl acetate.
1. Chromatographic conditions are as follows:
a chromatographic column: waters
C18(4.6 × 250mm,5 μm), detection wavelength 291nm, flow rate 1.0ml/min, column temperature 35 ℃, mobile phase A acetonitrile, mobile phase B potassium dihydrogen phosphate buffer solution 0.01mol/L (adjusted to pH4.0 with 20% potassium hydroxide solution);
the mobile phase adopts a gradient elution mode, and the gradient elution is carried out according to the following table:
table 7: gradient elution chart
2. Preparation of relevant solution and mobile phase:
2.1 preparation of the relevant solutions
Preparation of 2.1.120% Potassium hydroxide solution
20g of potassium hydroxide is weighed, dissolved in water and diluted to 100 ml.
Preparation of 2.1.20.01 mol/L potassium dihydrogen phosphate buffer solution
1.3609g of monopotassium phosphate was weighed, dissolved in water and diluted to 1000ml, and the pH was adjusted to 4.0 with 20% potassium hydroxide.
2.1.3 preparation of test solutions
Precisely weighing a proper amount of sodium p-methyl phenylhydrazine sulfonate, adding acetonitrile to dissolve, and quantitatively diluting to prepare a solution containing about 0.2mg of sodium p-methyl phenylhydrazine sulfonate in 1ml of the solution as a test solution.
2.2 preparation of the Mobile phase
Mixing the 0.01mol/L potassium dihydrogen phosphate buffer solution and acetonitrile according to the volume ratio of gradient elution to be used as a mobile phase.
3. The method for measuring the sodium p-methyl phenylhydrazine sulfonate related substances comprises the following steps:
and (3) taking 20 mu l of test solution, injecting into a liquid chromatograph, recording a color spectrum, and measuring by an area normalization method.
4. The result of the detection
13.35 percent of single impurity
Total impurities 100% -86.45% ~ 13.55%
EXAMPLE six
And (3) measuring related substances in the cinnamyl hydrazine serving as an intermediate for synthesizing the cinnamyl metacin.
1. Chromatographic conditions are as follows:
a chromatographic column: waters
C18(4.6 × 250mm,5 μm), detection wavelength 291nm, flow rate 1.0ml/min, column temperature 35 ℃, mobile phase A acetonitrile, mobile phase B potassium dihydrogen phosphate buffer solution 0.01mol/L (adjusted to pH4.0 with 20% potassium hydroxide solution);
the mobile phase adopts a gradient elution mode, and the gradient elution is carried out according to the following table:
table 8: gradient elution chart
2. Preparation of relevant solution and mobile phase:
2.1 preparation of the relevant solutions
Preparation of 2.1.120% Potassium hydroxide solution
20g of potassium hydroxide is weighed, dissolved in water and diluted to 100 ml.
Preparation of 2.1.20.01 mol/L potassium dihydrogen phosphate buffer solution
1.3609g of monopotassium phosphate was weighed, dissolved in water and diluted to 1000ml, and the pH was adjusted to 4.0 with 20% potassium hydroxide.
2.1.3 preparation of test solutions
Accurately weighing appropriate amount of cinnamyl hydrazine, adding acetonitrile to dissolve, and quantitatively diluting to obtain solution containing about 0.2mg per 1ml as test solution.
2.2 preparation of the Mobile phase
Mixing the 0.01mol/L potassium dihydrogen phosphate buffer solution and acetonitrile according to the volume ratio of gradient elution to be used as a mobile phase.
3. The method for measuring the related substances of cinnamyl hydrazine comprises the following steps:
and (3) taking 20 mu l of test solution, injecting into a liquid chromatograph, recording a color spectrum, and measuring by an area normalization method.
4. The result of the detection
1.07% of single impurity
The total impurities are 100% -96.96% ═ 3.04%.
Claims (11)
1. A method for detecting related substances in a cinnamyl octane related product by high performance liquid chromatography is characterized by comprising the following steps: the detection method comprises the following steps:
(1) chromatographic conditions are as follows:
a chromatographic column: chromatographic column with octadecylsilane chemically bonded silica as filler;
mobile phase: the mobile phase A is acetonitrile;
the mobile phase B is potassium dihydrogen phosphate buffer solution;
the mobile phase adopts a gradient elution mode;
the specific method of gradient elution is as follows: 0 minute, mobile phase a is 30%, mobile phase B is 70%; for 6 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent; 26 minutes, 70% of mobile phase A and 30% of mobile phase B; 30 minutes, 70% of mobile phase A and 30% of mobile phase B; for 40 minutes, the content of the mobile phase A is 30 percent, and the content of the mobile phase B is 70 percent; for 50 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent;
(2) preparation of a test solution:
accurately weighing a proper amount of sample, adding acetonitrile to dissolve and dilute the sample to be used as a test solution;
(3) preparation of control solution:
precisely measuring a proper amount of a test solution, and diluting the test solution with a mobile phase to be used as a reference solution;
(4) the determination method comprises the following steps:
injecting the reference solution into a liquid chromatograph, and adjusting the detection sensitivity to make the peak height of the main component chromatographic peak be 20% of the full-scale range; and precisely measuring the test solution and the reference solution, injecting the test solution and the reference solution into a liquid chromatograph, recording a map, measuring by using a main component self-reference method, wherein the peak area of a single impurity is not more than 1.5 times of the main peak area of the reference solution, the sum of the peak areas of all impurities is not more than 2 times of the main peak area of the reference solution, and calculating the content of the single impurity and the total impurity.
2. A method for detecting related substances in a cinnamyl octane related product by high performance liquid chromatography is characterized by comprising the following steps: the detection method comprises the following steps:
(1) chromatographic conditions are as follows:
a chromatographic column: chromatographic column with octadecylsilane chemically bonded silica as filler;
mobile phase: the mobile phase A is acetonitrile;
mobile phase B, potassium dihydrogen phosphate buffer solution:
the mobile phase adopts a gradient elution mode;
the specific method of gradient elution is as follows: 0 minute, mobile phase a is 30%, mobile phase B is 70%; for 6 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent; 26 minutes, 70% of mobile phase A and 30% of mobile phase B; 30 minutes, 70% of mobile phase A and 30% of mobile phase B; for 40 minutes, the content of the mobile phase A is 30 percent, and the content of the mobile phase B is 70 percent; for 50 minutes, the content of mobile phase A is 30 percent, and the content of mobile phase B is 70 percent;
(2) preparation of a test solution:
accurately weighing a proper amount of sample, adding acetonitrile to dissolve and dilute the sample to be used as a test solution;
(3) the determination method comprises the following steps:
and (3) injecting the test solution into a liquid chromatograph, recording a color spectrum, measuring by using an area normalization method, and calculating the content of single impurities and total impurities.
3. The method for detecting related substances in the cinnamyl octane related product by high performance liquid chromatography according to claim 1 or 2, wherein the method comprises the following steps: the mobile phase B is potassium dihydrogen phosphate buffer aqueous solution, and the pH value is adjusted to 4.0 by potassium hydroxide aqueous solution.
4. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 3, wherein the method comprises the following steps: the mobile phase B is 0.01mol/L potassium dihydrogen phosphate buffer aqueous solution, and the pH value is adjusted to 4.0 by 20 percent potassium hydroxide aqueous solution.
5. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 1, wherein the method comprises the following steps: in the step (2), the sample is a finished product of the cinnamyl acetate; the sample amount of the control solution is 10-100 mul; the preparation method of the control solution comprises the following steps: precisely measuring 1ml of the test solution, placing the test solution into a 100ml measuring flask, diluting the test solution to a scale with a mobile phase, and shaking up.
6. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 5, wherein the method comprises the following steps: the control solution was loaded in an amount of 20. mu.l.
7. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 2, wherein the method comprises the following steps: in the step (2), the sample is a raw material for synthesizing the cinnamyl acetate or an intermediate for synthesizing the cinnamyl acetate; the raw material for synthesizing the cinnamyl methyl ether comprises p-anisidine, and the intermediate for synthesizing the cinnamyl methyl ether comprises sodium p-methyl phenyl hydrazine sulfonate and cinnamyl hydrazine.
8. The method for detecting related substances in the cinnamyl octane related product by high performance liquid chromatography according to claim 1 or 2, wherein the method comprises the following steps: the detector of the detection method is an ultraviolet detector, the detection wavelength of the ultraviolet detector is 291nm, the flow rate of the mobile phase is 0.8ml/min-1.2ml/min, the column temperature is 25 ℃ to 45 ℃, and the sample volume of the sample solution is 10 mul-100 mul.
9. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 8, wherein the method comprises the following steps: the flow rate of the mobile phase is 1.0ml/min, the column temperature is 35 ℃, and the sample injection amount of the sample solution is 20 mu l.
10. The method for detecting related substances in the cinnamyl octane related product by high performance liquid chromatography according to claim 1 or 2, wherein the method comprises the following steps: the preparation method of the test solution comprises the steps of precisely weighing a proper amount of the test, adding acetonitrile to dissolve the test solution, and quantitatively diluting the test solution to prepare a solution containing 0.2mg of acetonitrile in each 1ml of the test solution to serve as the test solution.
11. The method for detecting related substances in the Guimeixin related products by high performance liquid chromatography according to claim 1, wherein the method comprises the following steps: in the method, the calculation method of the single impurities and the total impurities comprises the following steps:
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| WO2006004903A2 (en) * | 2004-06-28 | 2006-01-12 | Atherogenics, Inc. | 1,2-bis-(substituted-phenyl)-2-propen-1-ones and pharmaceutical compositions thereof |
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| CN102964287A (en) * | 2012-11-13 | 2013-03-13 | 苏州永健生物医药有限公司 | New synthesis method of 3-(4-chlorobutyl)-5-cyanoindole |
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| WO2006004903A2 (en) * | 2004-06-28 | 2006-01-12 | Atherogenics, Inc. | 1,2-bis-(substituted-phenyl)-2-propen-1-ones and pharmaceutical compositions thereof |
| CN101036632A (en) * | 2005-09-26 | 2007-09-19 | 刘凤鸣 | Sustained release preparation of cinmetacin |
| CN102964287A (en) * | 2012-11-13 | 2013-03-13 | 苏州永健生物医药有限公司 | New synthesis method of 3-(4-chlorobutyl)-5-cyanoindole |
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