CN109060688A - A kind of high-precision nano-sensor and its application - Google Patents
A kind of high-precision nano-sensor and its application Download PDFInfo
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- CN109060688A CN109060688A CN201810982572.2A CN201810982572A CN109060688A CN 109060688 A CN109060688 A CN 109060688A CN 201810982572 A CN201810982572 A CN 201810982572A CN 109060688 A CN109060688 A CN 109060688A
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- sample cell
- aureus
- reciprocating pump
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- forms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The present invention relates to a kind of high-precision nano-sensor and its applications.It includes spectrophotometer and sample cell;Sample cell is the glass dish that lower half endoperidium has resin nano particle;Resin nano particle the preparation method comprises the following steps: taking quasi- tsaoko ester, sodium hydroxide, heating, cool down after melting, adds polymethyl methacrylate, constant temperature, nanometer silica flour is added, it is uniformly dispersed, adds and carry out pre-deaeration in agitator, place into and carry out vacuum defoamation in vacuum drying oven, it is then injected into glass dish, is put into baking oven and is solidified.Compared with prior art, the present invention can be combined using resin nano particle with combination coagulase unique on S. aureus L-forms cell wall, and can both eliminate other impurity effects under the effect of 75% ethyl alcohol the characteristics of desorption, also inactive S. aureus L-forms are eliminated, it is higher from measurement result precision;Moreover, specific surface area can be expanded using nano shape, both favorably there is quick adsorption, also favorably there is fast desorption.
Description
Technical field
The invention belongs to sensor technical fields, more particularly to a kind of for surveying the high-precision nanosensor of S. aureus L-forms
Device.
Background technique
The development of current nanotechnology, only sensor does not provide good sensitive material, such as nanoparticle, nanometer
Pipe, nano wire, nano thin-film etc., and many novel designs and method, such as nanotechnology are provided for sensor production
In key technology STM, MEMS technology etc. of the research object to nanoscale transition.Compared with traditional sensor, nanometer is passed
The performances such as sensor size reduces, precision improves substantially improve, it is often more important that make sensor using nanotechnology, be station in original
On sub- scale, to be greatly enriched the theory of sensor, the production technique of sensor is pushed, has widened answering for sensor
Use field.
The main application fields of nano-sensor include health care, military affairs, Industry Control and robot, network and communication
And environmental monitoring etc..With the maturation of the relevant technologies, powerful advantages of the nano-sensor in terms of national defence safety check gradually show.
It is believed that in the near future, nano-sensor will be used for the military uniform and equipment of a new generation, and will be used to detect anthrax and others
Hazardous gas etc..Nano-sensor mainly includes following three categories: nano biological and chemical sensor, gas nanosensor,
With other kinds of nano-sensor (flow, pressure and temperature) etc..
S. aureus L-forms (staphylococcus aureus) are gram-positive bacteria, diameter about 1.0um.Staphylococcus aureus is the mankind
The most common pathogen in suppurative infection, can cause local suppurative infection, can also cause pneumonia, pseudomembranous enteritis, pericarditis etc.,
The even general infections such as septicemia, pyemia.Other than causing infection, the enterotoxin generated can pollute staphylococcus aureus
Food and cause food poisoning food easily polluted by it.Therefore, to staphylococcus aureus detect, limit the quantity health pacify
Seem particularly important in complete.It is main at present using in the presence of time-consuming too long and work for the quantitative detection of staphylococcus aureus
Measure big defect.Though spectrophotometry is relatively simple, the life or death of staphylococcus aureus cannot be distinguished.Moreover, existing measurement
Apparatus structure is complicated, the also less research in terms of sensor.
Summary of the invention
The invention discloses a kind of for surveying the high-precision nano-sensor of S. aureus L-forms, can overcome staphylococcus aureus
When quantitative detection the technical issues of time-consuming too long and heavy workload, and staphylococcus aureus living can be quantified.
High-precision nano-sensor of the present invention, the nano-sensor include spectrophotometer and sample cell;Institute
Stating sample cell is the glass dish that lower half endoperidium has resin nano particle;The resin nano particle the preparation method comprises the following steps: taking
Quasi- tsaoko ester 10g, sodium hydroxide 3g, are heated to 180 DEG C, 150 DEG C are cooled to after melting, adds polymethyl methacrylate
100g, constant temperature 1h are added nanometer silica flour 15g, are uniformly dispersed, add and carry out pre-deaeration in agitator, be put into later
Vacuum defoamation is carried out in vacuum drying oven, then fast spraying implantation glass ware, make its partial size in 5-20nm, be put into baking oven and carry out
Solidification.
High-precision nano-sensor of the present invention, the lower mouth closing of the sample cell, connects capillary, only with sucking
Sample or 75% ethanol solution;Its closing suitable for reading, only connects reciprocating pump, inhales sample volume with control.
High-precision nano-sensor of the present invention, the spectrophotometer include protective shell, are equipped in the protective shell
Battery, visible light source, entrance slit, collimating mirror, grating, condenser, exit slit, reflective mirror, photoelectric detector, A/D turn
Parallel operation, microprocessor, reciprocating pump, capillary;The battery is separately connected microprocessor, visible light source and reciprocating pump;It is described
The luminous energy that visible light source issues passes through entrance slit, and collimated mirror is converted into directional light, then exposes to grating and be divided, this point
Exit slit becomes monochromatic light to light again after condenser becomes light beam;The monochromatic light horizontal irradiation is to sample cell, and face sample
Photodiode display behind product pond;The microprocessor connects and controls photodiode display, A/D converter and reciprocal
Pump.
High-precision nano-sensor of the present invention, the visible light source are deuterium lamp or tungsten lamp;The Photoelectric Detection
Device is diode array;The protective shell of the sample cell corresponding position is transparent, to observe solution conditions.
High-precision nano-sensor of the present invention, the protective shell are in gun shaped, set and have microprocessor company on pikestaff
Two buttons connect control the operation and closing of reciprocating pump, visible light source respectively.
High-precision nano-sensor of the present invention, the assay for S. aureus L-forms.
The method that nano-sensor of the present invention is used to measure S. aureus L-forms, specific steps are as follows: press reciprocating pump and move button, make
Reciprocating pump up moves, and aspirated specimens, S. aureus L-forms are attracted in sample cell, by lower button, moves reciprocating pump down, except gold
Outside Portugal bacterium, other solution and solute are discharged outside sample cell;Button press again moves reciprocating pump up, aspirates 75% ethyl alcohol,
Desorb resin nano particle in conjunction with coagulase, so that S. aureus L-forms be made to become suspension;Visible light source button is pressed, is selected
Wavelength is 450nm, measures absorbance.
Working principle: S. aureus L-forms living can secrete kind of the unique combination coagulase for being incorporated in own cells wall.In sample cell
Lower half is coated with resin nano particle, makes the resin nano granular absorption combination coagulase, so as to adsorb S. aureus L-forms.Press by
Button moves reciprocating pump up, and aspirated specimens, S. aureus L-forms are attracted in sample cell, by lower button, transports reciprocating pump up
Dynamic, in addition to S. aureus L-forms, other solution and solute are discharged outside sample cell;Button press again moves reciprocating pump up, suction
75% ethyl alcohol desorbs resin nano particle in conjunction with coagulase.S. aureus L-forms become suspension, can measure suction at 450 nm
Luminosity.
Compared with prior art, high-precision nano-sensor of the present invention uses resin nano particle, utilizes tree
Rouge nano particle can be combined with combination coagulase unique on S. aureus L-forms cell wall, and can 75% ethyl alcohol effect under desorption
The characteristics of, other impurity effects had both been eliminated, inactive S. aureus L-forms are also eliminated, it is higher from measurement result precision;Moreover, adopting
With nano shape, specific surface area can be expanded, both favorably have quick adsorption, also favorably there is fast desorption.
Detailed description of the invention
Fig. 1: nano-sensor structural schematic diagram of the present invention.1- battery, 2- visible light source, 3- entrance slit,
4- collimating mirror, 5- grating, 6- condenser, 7- exit slit, 8- reflective mirror, 9- sample cell, 10- photodiode display, 11-A/
D converter, 12- microprocessor, 13- reciprocating pump, 14- capillary, 15- protective shell.
Specific embodiment
High-precision nano-sensor of the present invention is described further below with reference to specific embodiment, but this
The protection scope of invention is not limited to this.
Embodiment 1
High-precision nano-sensor, including spectrophotometer and sample cell;The sample cell is that lower half endoperidium has resin to receive
The glass dish of rice grain;The resin nano particle the preparation method comprises the following steps: taking quasi- tsaoko ester (2 (E) -2- decylene-1s, 10- diethyl
Acid esters) 10g, sodium hydroxide 3g, 180 DEG C are heated to, 150 DEG C are cooled to after melting, adds polymethyl methacrylate
100g, constant temperature 1h are added nanometer silica flour 15g, are uniformly dispersed, add and carry out pre-deaeration in agitator, place into true
Vacuum defoamation is carried out in empty baking oven, then fast spraying implantation glass ware, make its partial size in 5-20nm, be put into baking oven and consolidated
Change.The lower mouth of the sample cell is closed, and connects capillary, only to suck sample or 75% ethanol solution;Its closing suitable for reading, only connects
Reciprocating pump is connect, sample volume is inhaled with control.The spectrophotometer includes protective shell, and battery, visible light are equipped in the protective shell
Source, entrance slit, collimating mirror, grating, condenser, exit slit, reflective mirror, photoelectric detector, A/D converter, microprocessor,
Reciprocating pump, capillary;The battery is separately connected microprocessor, visible light source and reciprocating pump;What the visible light source issued
Luminous energy passes through entrance slit, and collimated mirror is converted into directional light, then exposes to grating and be divided, which becomes through condenser
Exit slit becomes monochromatic light again after light beam;The monochromatic light horizontal irradiation is to sample cell, and the photoelectricity two after face sample cell
Pole pipe display;The microprocessor connects and controls photodiode display, A/D converter and reciprocating pump.The visible light source
For deuterium lamp;The photoelectric detector is diode array;The protective shell of the sample cell corresponding position is transparent, molten to observe
Liquid situation.The protective shell is in gun shaped, two buttons for setting on pikestaff and having microprocessor to connect, and controls reciprocating pump respectively, can
The operation and closing in light-exposed source.
High-precision nano-sensor of the present invention, the assay for S. aureus L-forms.The nano-sensor is used for
The method for measuring S. aureus L-forms, step are as follows: press reciprocating pump and move button, move reciprocating pump up, aspirated specimens, S. aureus L-forms are adsorbed
In sample cell, by lower button, move reciprocating pump down, in addition to S. aureus L-forms, other solution and solute are discharged outside sample cell;
Button press again moves reciprocating pump up, aspirates 75% ethyl alcohol, desorbs resin nano particle in conjunction with coagulase, from
And S. aureus L-forms is made to become suspension;Visible light source button is pressed, selects wavelength for 450nm, measures absorbance.
Precise verification
Staphylococcus aureus is taken, after passing on twice, fraction bacterium is taken to be inoculated into plate progress, picking colony is configured to series
Concentration surveys absorbance under 450nm wavelength, while calculating bacterium number with thrombocytometry.The result shows that absorbance 0.132~
In the range of 0.921, the concentration of staphylococcus aureus and absorbance are in preferable linear relationship, regression equation be y=
9.748x-0.1495(R²=0.9888)。
Now by above-mentioned concentration (in terms of absorbance, respectively 0.132,0.568,0.669,0.777,0.921) golden yellow
Staphylococcus is measured as follows: it takes in the high-precision nano-sensor for surveying S. aureus L-forms, presses reciprocating pump and move button,
Move reciprocating pump up, aspirated specimens, S. aureus L-forms are attracted in sample cell, by lower button, are moved reciprocating pump down, are removed
Outside S. aureus L-forms, other solution and solute are discharged outside sample cell;Button press again moves reciprocating pump up, aspirates 75% second
Alcohol desorbs resin nano particle in conjunction with coagulase, so that S. aureus L-forms be made to become suspension;Visible light source button is pressed,
It selects wavelength for 450nm, measures absorbance, as a result respectively 0.123,0.549,0.641,0.749,0.897, the rate of recovery exists
95% or more;There may be inactive staphylococcus aureus in above-mentioned sample, if it is considered that this method is to the fastidious of its,
The rate of recovery is then higher.
It is simultaneously the uniqueness for verifying this sample cell, quasi- tsaoko ester is replaced with into 2 (E) -2- decylene-1s, 10- glycol is another to make
At a sample cell.Same conditional operation, the result for measuring absorbance is respectively 0.008,0.006,0.006,0.007,
0.008, illustrate do not have adsorption capacity.
To verify this method to the eliminating ability of inactive staphylococcus aureus, above-mentioned five samples are taken, respectively
It is divided into two equal portions, 4 DEG C of an equal portions refrigerator saves, and another equal portions are killed in 100 DEG C of water-baths, then mixes this two equal portions, respectively
It is measured with conventional spectrophotometers and this sensor.The result shows that discovery, the result that conventional spectrophotometers are surveyed are as follows: 0.136,
0.572,0.681,0.792,0.993;The result of this sensor measurement are as follows: 0.065,0.281,0.339,0.391,0.471, point
It Wei not the half of result or so surveyed of conventional spectrophotometers.It follows that high-precision nano-sensor of the present invention and
Its measuring method, can not only accurately measure the content of S. aureus L-forms, and can also distinguish inactive.
Compared with prior art, high-precision nano-sensor of the present invention uses resin nano particle, utilizes tree
Rouge nano particle can be combined with combination coagulase unique on S. aureus L-forms cell wall, and can 75% ethyl alcohol effect under desorption
The characteristics of, other impurity effects had both been eliminated, inactive S. aureus L-forms are also eliminated, it is higher from measurement result precision;Moreover, adopting
With nano shape, specific surface area can be expanded, both favorably have quick adsorption, also favorably there is fast desorption.
Claims (7)
1. a kind of high-precision nano-sensor, which is characterized in that including spectrophotometer and sample cell;The sample cell is lower half
Portion's endoperidium has the glass dish of resin nano particle;The resin nano particle the preparation method comprises the following steps: taking quasi- tsaoko ester 10g, hydrogen
Sodium oxide molybdena 3g, is heated to 180 DEG C, and 150 DEG C are cooled to after melting, and adds polymethyl methacrylate 100g, and constant temperature 1h adds
Enter a nanometer silica flour 15g, be uniformly dispersed, add and carry out pre-deaeration in agitator, is put into vacuum drying oven and carries out later
Vacuum defoamation, then fast spraying implantation glass ware, makes its partial size in 5-20nm, is put into baking oven and is solidified.
2. high-precision nano-sensor according to claim 1, which is characterized in that the lower mouth of the sample cell is closed, only
Capillary is connected, to suck sample or 75% ethanol solution;Its closing suitable for reading, only connects reciprocating pump, inhales sample volume with control.
3. high-precision nano-sensor according to claim 2, which is characterized in that the spectrophotometer includes protection
Shell, be equipped in the protective shell battery, visible light source, entrance slit, collimating mirror, grating, condenser, exit slit, reflective mirror,
Photoelectric detector, A/D converter, microprocessor, reciprocating pump, capillary;The battery is separately connected microprocessor, visible light
Source and reciprocating pump;The luminous energy that the visible light source issues passes through entrance slit, and collimated mirror is converted into directional light, then exposes to light
Grid are divided, and exit slit becomes monochromatic light again after condenser becomes light beam for the light splitting;The monochromatic light horizontal irradiation is extremely
Sample cell, and the photodiode display after face sample cell;The microprocessor connects and controls photodiode display, A/
D converter and reciprocating pump.
4. high-precision nano-sensor according to claim 3, which is characterized in that the protective shell is in gun shaped, on pikestaff
If controlling the operation and closing of reciprocating pump, visible light source respectively with two buttons for thering is microprocessor to connect.
5. high-precision nano-sensor according to claim 4, which is characterized in that the visible light source is deuterium lamp or tungsten
Lamp;The photoelectric detector is diode array;The protective shell of the sample cell corresponding position is transparent.
6. high-precision nano-sensor according to claim 5, which is characterized in that the assay for S. aureus L-forms.
7. the method that nano-sensor according to claim 5 is used to measure S. aureus L-forms, which is characterized in that specific steps
Are as follows: it presses reciprocating pump and moves button, move reciprocating pump up, aspirated specimens, S. aureus L-forms are attracted in sample cell, by lower button,
Move reciprocating pump down, in addition to S. aureus L-forms, other solution and solute are discharged outside sample cell;Button press again makes reciprocating pump
It up moves, aspirates 75% ethyl alcohol, desorb resin nano particle in conjunction with coagulase, be suspended so that S. aureus L-forms be made to become
Liquid;Visible light source button is pressed, selects wavelength for 450nm, measures absorbance.
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Application publication date: 20181221 |