CN109055481A - A kind of detection method of Acartia body surface particle-bound bacteria - Google Patents
A kind of detection method of Acartia body surface particle-bound bacteria Download PDFInfo
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- CN109055481A CN109055481A CN201810954637.2A CN201810954637A CN109055481A CN 109055481 A CN109055481 A CN 109055481A CN 201810954637 A CN201810954637 A CN 201810954637A CN 109055481 A CN109055481 A CN 109055481A
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- acartia
- body surface
- surface particle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Abstract
The invention discloses a kind of detection methods of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, specifically: pretreatment: the Acartia for taking target sea area to collect, it is placed on sterile strainer, with the cyclops on the sterile strainer of sterile ultrapure water, bacterium must not be detected by rinsing in the flushing water flowed out to strainer；Desorption: pretreatment gained Acartia being put into the centrifuge tube equipped with sterile water, desorption agent solution is added, and centrifugal treating again after ultrasonic treatment takes centrifuged supernatant, spare；Detection: by centrifuged supernatant obtained by desorption procedure, Bacteria Culture is carried out with the measuring method of total number of bacteria in drinking water standard method of inspection, the bacterium colony after culture is counted.Detection method operating procedure is simple, detection cycle is short, so that body surface particle-bound bacteria desorption is high-efficient, speed is fast, desorption is more thorough, strippant dosage is few, detection method accuracy is high, testing result is reliable and stable.
Description
Technical field
The present invention relates to technical field of microbial detection, more particularly, to a kind of detection of Acartia body surface particle-bound bacteria
Method.
Technical background
The widely distributed Yu Haiyang of Acartia is numerous economic fish and the main natural bait biology of the shrimp crab young.According to
FAO estimates, in sea fishery industries in 1999, it is the fish eaten that 0.92 hundred million tons of catch, which is with natural Copepods food organisms,
Class.Acartia category, widely distributed and China coast, Japanese coastal, Pacific and Indian oceans, are Zhoushan Fishing Ground and Adjacent Sea Area
One of the sociales of summer zooplankter are suitably applied culture fishery due to the features such as history of life is short, bait is secondary wide.It spins
The nutritive value n-3 highly unsaturated fatty acid (n-3HUPA) of hammer water flea is the essential fatty acid of marine fish larvae, they are seas
Necessary to water larva and juvenile normal growth, especially eicosapentenoic acid (EPA, 20:5n-3) and docosahexenoic acid (DHA, 22:
6n-3).The shortage of EPA and DHA or the insufficient growth and development that will affect the fishes and shrimps young, survival rate reduce, and vigor is insufficient, resistance
Weaken.Fish cannot synthesize linolenic acid (18:3n-3) own biological EPA and DHA, and required EPA and DHA can only be from
It is absorbed in feed.A large number of studies show that the copepods of many bank property (about account for contained fatty acid rich in DHA and EPA
60%), can satisfy demand of the larva and juvenile to HUFA.But the Acartia of natural sea area acquisition is due to carrying many cause a disease carefully
Bacterium directly feeds and easily the farming disease harms is caused to spread, therefore is badly in need of measuring the Carried bacteria situation of Acartia in advance.
The detection of invertebrate zooplankter body surface particle-bound bacteria in water is mainly used both at home and abroad at present
The method that Wolmarans.E is proposed.This method impregnates zooplankter with distilled water or physiological saline, passes through zooplankter body surface
Spontaneous release of the particle-bound bacteria into water obtains the sample of body surface particle-bound bacteria, can also use sometimes for release efficiency is increased
The supplementary means such as hand or shaking table oscillation.Nevertheless, the desorption efficiency of the zooplankter body surface particle-bound bacteria of such method is still
It is so not high, after desorbing water flea class zooplankter body surface particle-bound bacteria using such method, culture medium culture detection is carried out, there are water
The detected value of flea class zooplankter body surface particle-bound bacteria is relatively low and the drawbacks such as fluctuation is larger.Technology such as Authorization Notice No. is now
The Chinese invention patent of CN 103451263B discloses a kind of detection method of Cyclops of Zooplankton body surface particle-bound bacteria,
The following steps are included: step 1, cyclops in water sampling is washed to no Detection of pathogenic bacteria；Step 2, by step 1 gained sword
Water flea is put into centrifuge tube, and strippant is added；Step 3, centrifuge tube in step 2 is put into centrifuge centrifugal treating；Step 4, it takes
Supernatant after centrifugation, the supernatant after taking centrifugation, with the measuring method of total number of bacteria in drinking water standard method of inspection into
Row Bacteria Culture, the bacterium colony after culture are counted.The method operating procedure is simple, and detection cycle is short, swims for Cyclops
The detection effect of animal body surface particle-bound bacteria is significant.But the detection method is to the solution of water flea class zooplankter body surface particle-bound bacteria
It is not still too ideal for inhaling effect.
Summary of the invention
That one of the objects of the present invention is to provide a kind of operating procedures is simple, detection cycle is short, so that body surface particle-bound bacteria
Be desorbed high-efficient, speed is fast, desorption more thoroughly, strippant dosage it is few, detection method accuracy is high, testing result is reliable and stable
Acartia body surface particle-bound bacteria detection method.
The present invention in background technique aiming at the problem that mentioning, the technical solution taken are as follows:
A kind of detection method of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, wherein desorption procedure
Are as follows: pretreatment gained Acartia is put into the 50mL centrifuge tube equipped with 25mL sterile water, it is molten that 0.8-1.2mL strippant is added
Liquid is ultrasonically treated 12-15min under the ultrasonic power of 330-380W, then again at 1800-2300rpm, 20-30 DEG C from
The heart handles 2-5min, and centrifuged supernatant is taken after centrifugation, spare.Desorption procedure of the present invention uses strippant, ultrasonication
The mode combined with centrifugal treating desorbs Acartia body surface particle-bound bacteria, compared with the prior art, so that body surface adheres to
Bacterium desorption is high-efficient, speed is fast, desorption is more thorough, strippant dosage is few, so that detection method accuracy
Height, testing result are reliable and stable, and the detection method operating procedure is simple, detection cycle is short, for Acartia class zooplankter
The detection effect of body surface particle-bound bacteria is significant.
Preferably, strippant surfactant includes rhamnolipid and Tween 80.Acartia body surface particle-bound bacteria
Rely primarily on microorganism secretion extracellular polymeric and the biomembrane that is formed is adhered to Acartia body surface, and rhamnolipid and tween
80 reasonable exists and gain effect can occur with the other compositions in strippant, can hydrophilic film on the outside of disrupting biofilm,
And then the space three-dimensional structure of disrupting biofilm, promote the disintegration of bacterial biof iotalm, then cooperate centrifugal action, so that biomembrane
It falls off and disintegrates, and then its internal microorganism is enable to be disseminated in release agent completely, improve the accurate of detection method
Property.
Further preferably, surfactant also includes D- rhamnose, rhamnolipid, Tween 80 and D- mouse in surfactant
The mass ratio of Lee's sugar is 1:10-12:0.03-0.05.The rhamnolipid, Tween 80 of specific proportions and D- mouse in the surfactant
The mixing of Lee's sugar combines the precipitating/adsorption loss for significantly reducing anion nonionic surfactant, and improving surfactant has
Concentration is imitated, to cooperate with enhancing desorption water flea body surface particle-bound bacteria, enhances the desorption of Acartia body surface particle-bound bacteria, significantly subtracts
Few strippant dosage, while can cooperate with and increase particle-bound bacteria concentration in the solution, improve the resolution of particle-bound bacteria.
Preferably, also containing D- amino acid in strippant.D- amino acid can occupy the hydrophobic point of bacterium cousin, change thin
Bacterium surface hydrophobic, raising has the bacterium surface hydrophily released in biomembrane and Zeta potential absolute value, so that spindle water
Flea body surface particle-bound bacteria is quickly dissolved in wherein under the action of release agent, and D- amino acid is not obvious to the growth activity of bacterium
It influences, can guarantee the stability and accuracy of testing result.
Further preferably, D- amino acid is selected from D-Tyrosine, D-Leu, D-Ile, D-phenylalanine or D- first
Methyllanthionine.
For optimisation technique method, the measure taken further include: strippant specifically includes following ingredient and its parts by weight: chlorine
Change sodium 0.8-1.0 parts, 0.03-0.05 parts of surfactant, 0.1-0.25 parts of disodium hydrogen phosphate, 0.2-0.4 parts of potassium dihydrogen phosphate,
0.01-0.02 parts of D- amino acid.Synergistic effect can be played in the strippant between each effective component, under conditions of small amount effect
Acartia body surface particle-bound bacteria can be made to realize and desorb thorough, high-efficient desorption.
Preferably, the concentration of desorption agent solution is 1.14-1.72%.
Preferably, pretreated specific steps step are as follows: the Acartia for taking target sea area to collect is placed in sterile strainer
On, with the cyclops on the sterile strainer of sterile ultrapure water, bacterium must not be detected by rinsing in the flushing water flowed out to strainer.
Rinsing the cyclops that target sea area is collected is in order to avoid the free bacterium in sampling process in water sample adheres to cyclops body surface
The influence of Bacteria Detection, the pre-treatment step can make free bacterium cleaning thoroughly, shorten scavenging period, while avoided again clear
Body surface bacterium excessively loses during washing.
Preferably, the specific steps step of detection are as follows: by centrifuged supernatant obtained by desorption procedure, with Drinking Water mark
The measuring method of total number of bacteria carries out Bacteria Culture in quasi- method of inspection, and the bacterium colony after culture is counted.
Further preferably, pretreatment and Bacteria Detection in detecting step are according to total number of bacteria in GB/T5750.12-2006
Measuring method carry out.
Compared with the prior art, the advantages of the present invention are as follows: 1) detection method operating procedure simple, detection cycle
Short, significant for the detection effect of Acartia class zooplankter body surface particle-bound bacteria, accuracy height, testing result stabilization can
It leans on；2) present invention desorption step so that the high-efficient, speed of body surface particle-bound bacteria desorption is fast, desorption more thoroughly, strippant dosage it is few,
So that detection method accuracy is high, testing result is reliable and stable；3) each effective component in strippant of the invention
Between can play synergistic effect, small amount effect under conditions of can make Acartia body surface particle-bound bacteria realize desorption thoroughly,
High-efficient desorption can guarantee the stability and accuracy of testing result；4) detection method is by being suitably modified
The other zooplankter Endophytic bacterias of detection can be spent, the scope of application is wider, and practicability is stronger, operability with higher, fits
Conjunction is widely popularized and applies.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of detection method of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, wherein desorption procedure
Are as follows: pretreatment gained Acartia is put into the 50mL centrifuge tube equipped with 25mL sterile water, 0.8mL is added and desorbs agent solution,
12min is ultrasonically treated under the ultrasonic power of 330W, then centrifugal treating 2min, centrifugation terminate at 1800rpm, 20 DEG C again
After take centrifuged supernatant, it is spare.The desorption procedure is in such a way that strippant, ultrasonication and centrifugal treating combine to spinning
Hammer water flea body surface particle-bound bacteria is desorbed, compared with the prior art, so that body surface particle-bound bacteria desorption is high-efficient, speed is fast, desorption
More thoroughly, strippant dosage is few, so that detection method accuracy is high, testing result is reliable and stable, and the detection method is grasped
Make that step is simple, detection cycle is short, it is significant for the detection effect of Acartia class zooplankter body surface particle-bound bacteria.
Above-mentioned strippant surfactant includes rhamnolipid and Tween 80.Acartia body surface particle-bound bacteria mainly according to
The biomembrane formed by microorganism secretion extracellular polymeric is adhered to Acartia body surface, and the conjunction of rhamnolipid and Tween 80
Reason exists and gain effect can occur with the other compositions in strippant, can hydrophilic film on the outside of disrupting biofilm, and then it is broken
The space three-dimensional structure of bad biomembrane promotes the disintegration of bacterial biof iotalm, then cooperates centrifugal action so that biofilm detachment and
Disintegrate, and then its internal microorganism is enable to be disseminated in release agent completely, improves the accuracy of detection method.
Above-mentioned surfactant also includes D- rhamnose, rhamnolipid in surfactant, Tween 80 and D- rhamnose
Mass ratio is 1:10:0.03.The mixing of the rhamnolipid, Tween 80 and D- rhamnose of specific proportions combines in the surfactant
Precipitating/adsorption loss of anion nonionic surfactant is significantly reduced, surfactant effective concentration is improved, to cooperate with
Enhancing desorption water flea body surface particle-bound bacteria, enhances the desorption of Acartia body surface particle-bound bacteria, substantially reduces strippant dosage, together
When can cooperate with and increase particle-bound bacteria concentration in the solution, improve the resolution of particle-bound bacteria.
Also contain D- amino acid in above-mentioned strippant.D- amino acid can occupy the hydrophobic point of bacterium cousin, change bacterium table
Face hydrophobicity, raising has the bacterium surface hydrophily released in biomembrane and Zeta potential absolute value, so that Acartia body
Table particle-bound bacteria is quickly dissolved in wherein under the action of release agent, and D- amino acid does not have obvious shadow to the growth activity of bacterium
It rings, can guarantee the stability and accuracy of testing result.
Wherein, D- amino acid is selected from D-Tyrosine, D-Leu, D-Ile, D-phenylalanine or D-Met.
Strippant specifically includes following ingredient and its parts by weight: 0.8 part of sodium chloride, 0.03 part of surfactant, phosphoric acid hydrogen
0.1 part of disodium, 0.2 part of potassium dihydrogen phosphate, 0.01 part of D-Tyrosine.Collaboration can be played in the strippant between each effective component to make
With the desorption that can make the realization desorption of Acartia body surface particle-bound bacteria thorough, high-efficient under conditions of small amount effect.
Wherein, the concentration for desorbing agent solution is 1.14%.
Pretreated specific steps step are as follows: the Acartia for taking target sea area to collect is placed on sterile strainer, use is sterile
Cyclops on the sterile strainer of ultrapure water, bacterium must not be detected by rinsing in the flushing water flowed out to strainer.Rinse target
The cyclops that sea area is collected is in order to avoid the free bacterium in sampling process in water sample detects cyclops body surface particle-bound bacteria
Influence, which can make the cleaning of free bacterium thoroughly, shorten scavenging period, while avoiding again in the process of cleaning
Body surface bacterium excessively loses.
The specific steps step of detection are as follows: by centrifuged supernatant obtained by desorption procedure, with drinking water standard method of inspection
The measuring method of middle total number of bacteria carries out Bacteria Culture, and the bacterium colony after culture is counted, as a result such as table 1.
Wherein, pretreatment and Bacteria Detection in detecting step are the measurements according to total number of bacteria in GB/T5750.12-2006
What method carried out.
Embodiment 2:
A kind of detection method of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, specifically:
1) pretreated specific steps: the Acartia for taking target sea area to collect is placed on sterile strainer, with sterile ultrapure
Water rinses the cyclops on sterile strainer, and bacterium must not be detected by rinsing in the flushing water flowed out to strainer；
2) it desorbs: pretreatment gained Acartia being put into the 50mL centrifuge tube equipped with 25mL sterile water, 1.0mL is added
The desorption agent solution that concentration is 1.45%, is ultrasonically treated 14min, then again in 2000rpm, 25 under the ultrasonic power of 350W
Centrifugal treating 3min at DEG C takes centrifuged supernatant after centrifugation, spare；
3) the specific steps step detected are as follows: by centrifuged supernatant obtained by desorption procedure, examined with drinking water standard
The measuring method of total number of bacteria carries out Bacteria Culture in method, and the bacterium colony after culture is counted, as a result such as table 1.
Wherein, strippant specifically includes following ingredient and its parts by weight: 0.9 part of sodium chloride, 0.04 part of surfactant, phosphorus
0.18 part of sour disodium hydrogen, 0.3 part of potassium dihydrogen phosphate, 0.015 part of D- amino acid.Rhamnolipid, tween in above-mentioned surfactant
The mass ratio of 80 and D- rhamnose is 1:11:0.04；D- amino acid is the D-Tyrosine and D-Leu that mass ratio is 1:0.25.
Wherein, pretreatment and Bacteria Detection in detecting step are the measurements according to total number of bacteria in GB/T5750.12-2006
What method carried out.
Embodiment 3:
A kind of detection method of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, specifically:
1) pretreated specific steps: the Acartia for taking target sea area to collect is placed on sterile strainer, with sterile ultrapure
Water rinses the cyclops on sterile strainer, and bacterium must not be detected by rinsing in the flushing water flowed out to strainer；
2) it desorbs: pretreatment gained Acartia being put into the 50mL centrifuge tube equipped with 25mL sterile water, 1.2mL is added
The desorption agent solution that concentration is 1.72%, is ultrasonically treated 15min, then again in 2300rpm, 30 under the ultrasonic power of 380W
Centrifugal treating 5min at DEG C takes centrifuged supernatant after centrifugation, spare；
3) the specific steps step detected are as follows: by centrifuged supernatant obtained by desorption procedure, examined with drinking water standard
The measuring method of total number of bacteria carries out Bacteria Culture in method, and the bacterium colony after culture is counted, as a result such as table 1.
Strippant specifically includes following ingredient and its parts by weight: 1.0 parts of sodium chloride, 0.05 part of surfactant, phosphoric acid hydrogen
0.25 part of disodium, 0.4 part of potassium dihydrogen phosphate, 0.02 part of D- amino acid.Rhamnolipid, Tween 80 and D- in above-mentioned surfactant
The mass ratio of rhamnose is 1:12:0.05；D- amino acid is D-phenylalanine.
Wherein, pretreatment and Bacteria Detection in detecting step are the measurements according to total number of bacteria in GB/T5750.12-2006
What method carried out.
Comparative example 1:
A kind of detection method of Acartia body surface particle-bound bacteria, desorption procedure specifically include following ingredient with strippant
And its parts by weight: 0.9 part of sodium chloride, 0.04 part of surfactant, 0.18 part of disodium hydrogen phosphate, 0.3 part of potassium dihydrogen phosphate, D- ammonia
0.015 part of base acid.The mass ratio of Tween 80 and D- rhamnose is 12:0.04 in above-mentioned surfactant；D- amino acid is quality
Than the D-Tyrosine and D-Leu for 1:0.25.Remaining step and embodiment 2 are completely the same, testing result such as table 1.
Comparative example 2:
A kind of detection method of Acartia body surface particle-bound bacteria, desorption procedure specifically include following ingredient with strippant
And its parts by weight: 0.9 part of sodium chloride, 0.04 part of surfactant, 0.18 part of disodium hydrogen phosphate, 0.3 part of potassium dihydrogen phosphate, D- ammonia
0.015 part of base acid.The mass ratio of rhamnolipid and D- rhamnose is 12:0.04 in above-mentioned surfactant；D- amino acid is matter
Amount is than the D-Tyrosine and D-Leu for 1:0.25.Remaining step and embodiment 2 are completely the same, testing result such as table 1.
Comparative example 3:
A kind of detection method of Acartia body surface particle-bound bacteria, desorption procedure specifically include following ingredient with strippant
And its parts by weight: 0.9 part of sodium chloride, 0.04 part of surfactant, 0.18 part of disodium hydrogen phosphate, 0.3 part of potassium dihydrogen phosphate, D- ammonia
0.015 part of base acid.Rhamnolipid in above-mentioned surfactant, Tween 80 mass ratio be 1:11；D- amino acid is that mass ratio is
1:0.25 D-Tyrosine and D-Leu.Remaining step and embodiment 2 are completely the same, testing result such as table 1.
Comparative example 4:
A kind of detection method of Acartia body surface particle-bound bacteria, desorption procedure specifically include following ingredient with strippant
And its parts by weight: 0.9 part of sodium chloride, 0.04 part of surfactant, 0.18 part of disodium hydrogen phosphate, 0.3 part of potassium dihydrogen phosphate.It is above-mentioned
The mass ratio of rhamnolipid, Tween 80 and D- rhamnose is 1:11:0.04 in surfactant.Remaining step and embodiment 2 are complete
It is complete consistent, testing result such as table 1.
The testing result of 1 Acartia body surface particle-bound bacteria of table
The data result of contrast table 1, it can be found that the bacterium of the attachment of cyclops body surface measured by method of the invention is total
Amount is significantly greater than the detection method of comparative example.Testing result of the present invention is stablized, 4 resulting data of parallel test relatively, often
Secondary testing result all differs 30CFU/ or more.Synergistic effect can be played between each effective component in strippant of the invention simultaneously,
Acartia body surface particle-bound bacteria can be made to realize under conditions of small amount effect and desorb thorough, high-efficient desorption.
Routine operation in operation of the present invention step is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of detection method of Acartia body surface particle-bound bacteria, including pretreatment, desorption, detection, it is characterised in that: described
Desorption procedure are as follows: pretreatment gained Acartia is put into the centrifuge tube equipped with sterile water, is added and desorbs agent solution, at ultrasound
Centrifugal treating again after reason, takes centrifuged supernatant, spare.
2. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: the solution
Vapor surfactant includes rhamnolipid and Tween 80.
3. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 2, it is characterised in that: the table
Face activating agent also includes D- rhamnose, and the mass ratio of rhamnolipid, Tween 80 and D- rhamnose is 1 in the surfactant:
10-12:0.03-0.05。
4. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: the solution
Also contain D- amino acid in vapor.
5. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 4, it is characterised in that: the D-
Amino acid is selected from D-Tyrosine, D-Leu, D-Ile, D-phenylalanine or D-Met.
6. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: the solution
Vapor specifically includes following ingredient and its parts by weight: 0.8-1.0 parts of sodium chloride, 0.03-0.05 parts of surfactant, phosphoric acid hydrogen two
0.1-0.25 parts of sodium, 0.2-0.4 parts of potassium dihydrogen phosphate, 0.01-0.02 parts of D- amino acid.
7. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: the solution
The concentration of vapor solution is 1.14-1.72%.
8. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: described pre-
Processing step are as follows: the Acartia for taking target sea area to collect is placed on sterile strainer, on the sterile strainer of sterile ultrapure water
Cyclops, bacterium must not be detected by rinsing in the flushing water that flows out to strainer.
9. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 1, it is characterised in that: the inspection
Survey step are as follows: by centrifuged supernatant obtained by desorption procedure, with the measuring method of total number of bacteria in drinking water standard method of inspection
Bacteria Culture is carried out, the bacterium colony after culture is counted.
10. a kind of detection method of Acartia body surface particle-bound bacteria according to claim 8 or claim 9, it is characterised in that: institute
Stating Bacteria Detection is carried out according to the measuring method of total number of bacteria in GB/T5750.12-2006.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102791262A (en) * | 2010-01-08 | 2012-11-21 | 哈佛大学校长及研究员协会 | D-amino acids for use in treating biofilms |
| CN103451263A (en) * | 2013-08-15 | 2013-12-18 | 河海大学 | Detection method for bacteria attached to body surfaces of cyclops zooplankton |
| CN104053632A (en) * | 2011-08-26 | 2014-09-17 | 俄亥俄州大学 | Compositions and methods for treating biofilms |
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2018
- 2018-08-21 CN CN201810954637.2A patent/CN109055481B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102791262A (en) * | 2010-01-08 | 2012-11-21 | 哈佛大学校长及研究员协会 | D-amino acids for use in treating biofilms |
| CN104053632A (en) * | 2011-08-26 | 2014-09-17 | 俄亥俄州大学 | Compositions and methods for treating biofilms |
| CN103451263A (en) * | 2013-08-15 | 2013-12-18 | 河海大学 | Detection method for bacteria attached to body surfaces of cyclops zooplankton |
Non-Patent Citations (1)
| Title |
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| 龙绛雪等: "生物-非离子型表面活性剂对土壤石油污染的清除", 《湖北农业科学》 * |
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