CN109055293A - A kind of screening of river regulation bacterial strain and acclimation method - Google Patents
A kind of screening of river regulation bacterial strain and acclimation method Download PDFInfo
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- CN109055293A CN109055293A CN201810885989.7A CN201810885989A CN109055293A CN 109055293 A CN109055293 A CN 109055293A CN 201810885989 A CN201810885989 A CN 201810885989A CN 109055293 A CN109055293 A CN 109055293A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 11
- 238000012216 screening Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 63
- 241000894006 Bacteria Species 0.000 claims abstract description 10
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 28
- 229910052698 phosphorus Inorganic materials 0.000 claims description 27
- 239000011574 phosphorus Substances 0.000 claims description 27
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 24
- 239000002609 medium Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 12
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 12
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 11
- 239000004202 carbamide Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 238000005070 sampling Methods 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 6
- 230000000813 microbial effect Effects 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 230000003068 static effect Effects 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000013589 supplement Substances 0.000 claims description 4
- 239000002344 surface layer Substances 0.000 claims description 4
- 239000010802 sludge Substances 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 10
- 238000005273 aeration Methods 0.000 description 3
- 238000005086 pumping Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Abstract
The present invention provides screening and the acclimation method of a kind of river regulation bacterial strain, obtains bacterium source by collecting water sample, enrichment flora, flora domestication, launches flora tame, completes to screen and tame.The present invention is a kind of not only economic but also efficiently river degrading microorganism screening and acclimation method, and flora can rapidly adapt to current river course environment, ecological safety after domestication, pollution-free.Bacterium source of the present invention be derived from pollution of river area and near, while without large scale equipment investment and reagent expense, it is at low cost, be conducive to promote, it is with strong points using one quasi-microorganism of a river；The flora filtered out through the method for the present invention is better than single plant degradation bacteria strains effect, each bacterial strain synergistic effect, efficient degradation inside flora；It can quickly be bred in contaminated area rapidly within flora 1-2 days through what the present invention filtered out, be especially suitable for the not runny rivers such as beheaded river, rapidly adapt to, it is high-efficient.
Description
Technical field
The invention belongs to marine organisms field, it is related to screening and the acclimation method of a kind of river regulation bacterial strain.
Background technique
As the concern of all sectors of society and the guidance of national policy are supported, the improvement of water pollution is carried out both at home and abroad
A section dirty pollution treatment, dilution water transfer, artificial flowing, artificial aeration, Sediment Dredging, original have gradually been found out in positive research and trial
The measures such as position covering, flocculation sedimentation.These measures, physical method cost is high, and effect is immediately obvious；Chemical method is at high cost, easily
Secondary pollution is generated, adverse effect easily is generated to the entire ecosystem, is usually used in burst pollution Emergency use；Water plant ecology
Reparation is widely adopted, but needs the enough transparencies of water body, is suitable as administering the water quality guardian in later period；Microbial cleaning
Technical fee saves, effect is good, few to external interference, without secondary pollution, has comprehensive benefit.Microorganism fundamentally accelerates water body
The decomposition and inversion of nutriment improves the purification efficiency of rivers and lakes, the joint that Purification biotechnology is combined with other technologies
Measure is the development trend of polluted-water Treatment process.
Summary of the invention
The object of the present invention is to provide a kind of screening of river regulation bacterial strain and acclimation methods, are realized by following steps:
1. bacterium source obtains
Water sampling uses in sterile blue mouth bottle hydrostatic-layer and collects water sample；The acquisition of sludge sample is that acquisition is placed in nothing from mud surface layer
In bacterium vessel.All samples interior for 24 hours should be sealed at enrichment culture or 4 DEG C.The COD of river water is measured simultaneously,
Ammonia nitrogen, total phosphorus index.
2. enriching floras
Water sampling and mud sample are put in enriched medium, carry out shaking table enrichment culture about under 25-30 DEG C, 140rpm
One time can be obtained generation pregnant solution.
The enriched medium needs addition nutriment to be taken in river water.Specific adding method, addition carbon source, nitrogen source,
Phosphorus source is into river water, until COD: ammonia nitrogen: total phosphorus=(200~500): 5:1, as enriched medium.
Glucose, urea and potassium dihydrogen phosphate can be selected in carbon source, nitrogen source, phosphorus source.
After the enrichment culture of the first step, according to generation pregnant solution: the addition enrichment of enriched medium=1:10 ratio
Liquid continues enrichment culture into enriched medium under 25-30 DEG C, 140rpm, after measured its COD, ammonia nitrogen, total phosphorus in culture medium
Index drop by half can be obtained two generation pregnant solutions.
Then two generation pregnant solutions is taken to be added in enriched medium according to the ratio of above-mentioned 1:10, at 25-35 DEG C, 140rpm
Lower continuation enrichment culture, until its COD in culture medium after measured, ammonia nitrogen, total phosphorus index drop by half, so far enrichment finishes to obtain richness
Collect flora.
3. flora is tamed
The flora being enriched with is added in the container for filling river water to be handled according to the ratio of volume ratio 1:100, simultaneously
According to COD: urea: potassium dihydrogen phosphate=(100-200): 5:1 addition urea and potassium dihydrogen phosphate nutriment, 25 DEG C of temperature control,
Air Exposure is carried out, dissolved oxygen 2mg/L or more is controlled, measures its COD in culture medium, ammonia nitrogen, total phosphorus index drop by half is static to put
1h is set, addition river water supplement after one third supernatant, institute after being supplemented river water after testing index drop by half again once are removed
Obtaining liquid is the microbial flora tamed.
4. flora is launched
The enrichment and domestication of general flora carry out near river, and the flora needs tamed are nearest, timely launch and arrive
In river to be processed, it is thrown to oxygen increasing equipment periphery as far as possible.
The present invention is a kind of not only economic but also efficiently river degrading microorganism screening and acclimation method, and flora is after domestication
Current river course environment, ecological safety can be rapidly adapted to, it is pollution-free.The features of the present invention mainly has: (1) one quasi-microorganism of a river,
It is with strong points: bacterium source be derived from pollution of river area and near, while without large scale equipment investment and reagent expense, it is at low cost, be conducive to
It promotes.(2) high-efficient: the flora filtered out is better than single plant degradation bacteria strains effect, and each bacterial strain synergistic effect, efficiently drops inside flora
Solution.(3) it rapidly adapts to: can quickly be bred in contaminated area rapidly within the flora generally filtered out 1-2 days, be especially suitable for beheaded river etc.
Not runny river.
Specific embodiment
The present invention is described further in conjunction with the embodiments.
Embodiment 1
1. bacterium source obtains
Water sampling is in the river Shen Jiabang of Jiaxing Jiashan, using collecting water sample about 500ml in sterile blue mouth bottle hydrostatic-layer；
The acquisition of sludge sample is to acquire about 0.5-1.0kg from mud surface layer to be placed in sterile vessel.All samples are sealed at 4 DEG C interior for 24 hours and are protected
It deposits.The COD of river water, ammonia nitrogen are measured simultaneously, and total phosphorus index is 120.5mg/L, 32.3mg/L, 2.8mg/L.
2. enriching floras
Addition glucose, urea and potassium dihydrogen phosphate are into river water to COD: ammonia nitrogen: total phosphorus=500:5:1 forms enrichment
Culture medium.Water sampling 10ml and mud sample 0.5g are put in 500ml enriched medium, and shaking table is carried out under 25 DEG C, 140rpm
About one time of enrichment culture.
After the enrichment culture of the first step, according to generation pregnant solution: the addition enrichment of enriched medium=1:10 ratio
Liquid continues enrichment culture into enriched medium under 25 DEG C, 140rpm, and shaking table culture, timing measures daily, cultivates after measured
Its COD in base, ammonia nitrogen, total phosphorus index, which is down in former river water, drops to 59mg/L, 5mg/L, 0.5mg/L, can be obtained two generations richness
Liquid collecting, about 5 liters.
Then two generation pregnant solutions are taken to be added in enriched medium according to the ratio of above-mentioned 1:10, at 25 DEG C, under 140rpm
Continue enrichment culture, shaking table culture, timing measures daily, its COD in culture medium, ammonia nitrogen, total phosphorus index are down to Yuan He after measured
54.4mg/L, 12.5mg/L, 0.9mg/L are dropped in water, so far enrichment finishes to obtain 50 liters of flora of enrichment.
3. flora domestication (two three generations domestication, launch ratio 1:10)
The flora being enriched with is added to according to the ratio of volume ratio 1:100 and is filled in river water to be handled, while according to
COD: urea: potassium dihydrogen phosphate=100:5:1 addition urea and potassium dihydrogen phosphate nutriment, carry out at aeration by 25 DEG C of temperature control
Reason controls dissolved oxygen 2mg/L or more, measures its COD in culture medium, ammonia nitrogen, total phosphorus index drop by half, static placement 1h, removal
River water supplement is added after one third supernatant, being supplemented the primary rear gained liquid of river water after testing index drop by half again is
The microbial flora tamed, about 5 tons of liquid.
4. flora is launched
Using pumping equipments such as water pumps, domestication flora is extracted into river, surveyed river dissolved oxygen in water is 1.5mg/L, temperature
23 DEG C, COD of degree, ammonia nitrogen, total phosphorus index are 120.5mg/L, 32.3mg/L, 2.8mg/L.Daily testing index situation of change, tool
Body is shown in Table 1.
Table 1
| Date | CODcr(mg/L) | Ammonia nitrogen (mg/L) | Total phosphorus (mg/L) | Remarks |
| 1st day | 120.5 | 32.3 | 2.8 | Bad five classes water |
| 7th day | 98.6 | 27.36 | 2.1 | Bad five classes water |
| 14th day | 87.4 | 17.01 | 1.03 | Bad five classes water |
| 21st day | 65.7 | 5.67 | 0.62 | Bad five classes water |
| 28th day | 60.4 | 2.56 | 0.45 | Bad five classes water |
| 35th day | 52.4 | 2.07 | 0.41 | Bad five classes water |
| 42nd day | 39.9 | 1.96 | 0.35 | Five class water |
Embodiment 2
1. bacterium source obtains
Water sampling Ha Noi city Chang Bang under Hangzhou, using collection water sample about 500ml in sterile blue mouth bottle hydrostatic-layer；It is dirty
Mud sample acquisition is to acquire about 1.0kg from mud surface layer to be placed in sterile vessel.All samples are sealed at interior 4 DEG C of progress for 24 hours.
The COD of river water, ammonia nitrogen are measured simultaneously, and total phosphorus index is 50.5mg/L, 15.3mg/L, 3.1mg/L.
2. enriching floras
Addition glucose, ammonium sulfate and potassium dihydrogen phosphate are into river water to COD: ammonia nitrogen: total phosphorus=300:5:1 is formed rich
Collect culture medium.Water sampling 150ml and mud sample 1g are put in 500ml enriched medium, and shaking table is carried out under 30 DEG C, 140rpm
About one time of enrichment culture.
After the enrichment culture of the first step, according to generation pregnant solution: the addition enrichment of enriched medium=1:10 ratio
Liquid continues enrichment culture into enriched medium under 30 DEG C, 140rpm, and shaking table culture, timing measures daily, cultivates after measured
Its COD in base, ammonia nitrogen, total phosphorus index, which is down in former river water, drops to 40mg/L, 7.2mg/L, 1.2mg/L, can be obtained for two generations
Pregnant solution, about 5 liters.
Then two generation pregnant solutions are taken to be added in enriched medium according to the ratio of above-mentioned 1:10, at 25 DEG C, under 140rpm
Continue enrichment culture, shaking table culture, timing measures daily, its COD in culture medium, ammonia nitrogen, total phosphorus index are down to Yuan He after measured
39.4mg/L, 2.5mg/L, 0.8mg/L are dropped in water, so far enrichment finishes to obtain 50 liters of flora of enrichment.
3. flora domestication (two three generations domestication, launch ratio 1:10)
The flora being enriched with is added to according to the ratio of volume ratio 1:100 and is filled in river water to be handled, while according to
COD: urea: potassium dihydrogen phosphate=300:5:1 addition urea and potassium dihydrogen phosphate nutriment, carry out at aeration by 30 DEG C of temperature control
It manages, control dissolved oxygen 2.2mg/L or more, its COD in measurement culture medium, ammonia nitrogen, total phosphorus index drop by half, static placement 1h is gone
Except river water supplement is added after one third supernatant, the primary rear gained liquid of river water is supplemented after testing index drop by half again i.e.
For the microbial flora tamed, about 5 tons of liquid.
4. flora is launched
Using pumping equipments such as water pumps, domestication flora is extracted into river, surveyed river dissolved oxygen in water is 2.1mg/L, temperature
27 DEG C, COD of degree, ammonia nitrogen, total phosphorus index are 50.5mg/L, 15.3mg/L, 3.1mg/L.Daily testing index situation of change, specifically
It is shown in Table 2.
Table 2
| Date | CODcr(mg/L) | Ammonia nitrogen (mg/L) | Total phosphorus (mg/L) | Remarks |
| 1st day | 50.5 | 15.3 | 3.1 | Bad five classes water |
| 5th day | 49.6 | 8.56 | 2.8 | Bad five classes water |
| 8th day | 45.7 | 7.45 | 1.9 | Bad five classes water |
| 11st day | 33.5 | 5.67 | 2.5 | Bad five classes water |
| 14th day | 32.5 | 3.96 | 0.89 | Bad five classes water |
| 19th day | 16.4 | 2.68 | 1.56 | Bad five classes water |
| 23rd day | 15.2 | 1.78 | 0.35 | Five class water |
Claims (4)
1. screening and the acclimation method of a kind of river regulation bacterial strain, which is characterized in that realized by following steps:
(1) bacterium source obtains
Water sampling is that acquisition is placed in sterile device from mud surface layer using water sample, the acquisition of sludge sample is collected in sterile blue mouth bottle hydrostatic-layer
In ware, all samples interior for 24 hours should be sealed at enrichment culture or 4 DEG C, while measure the COD of river water, ammonia nitrogen,
Total phosphorus index；
(2) enriching floras
Water sampling and mud sample are put in enriched medium, when carrying out shaking table enrichment culture one under 25-30 DEG C, 140rpm
Between, to obtain generation pregnant solution；
(3) flora is tamed
The flora being enriched with is added in the container for filling river water to be handled according to the ratio of volume ratio 1:100, while according to
COD: urea: potassium dihydrogen phosphate=100~200:5:1 addition urea and potassium dihydrogen phosphate nutriment, are exposed by 25 DEG C of temperature control
Gas disposal, control dissolved oxygen 2mg/L or more, measure culture medium in its COD, ammonia nitrogen, total phosphorus index drop by half, static placement 1h,
Addition river water supplements after removing one third supernatant, is supplemented the primary rear gained liquid of river water after testing index drop by half again
The microbial flora as tamed；
(4) flora is launched
The enrichment and domestication of general flora carry out near river, and the flora tamed needs nearby, timely that dispensing be to will locate
In the river of reason, it is thrown to oxygen increasing equipment periphery as far as possible.
2. screening and the acclimation method of a kind of river regulation bacterial strain according to claim 1, which is characterized in that step (2)
The enriched medium needs addition nutriment to be taken in river water, specific adding method: addition carbon source, nitrogen source, phosphorus source to river
In water, until COD: ammonia nitrogen: total phosphorus=200~500:5:1, as enriched medium.
3. screening and the acclimation method of a kind of river regulation bacterial strain according to claim 2, which is characterized in that the carbon
Source, nitrogen source, phosphorus source select glucose, urea and potassium dihydrogen phosphate.
4. screening and the acclimation method of a kind of river regulation bacterial strain according to claim 2, which is characterized in that step (2)
It is described after the enrichment culture of the first step, according to generation pregnant solution: enriched medium=1:10 ratio addition pregnant solution arrive
In enriched medium, continue enrichment culture under 25-30 DEG C, 140rpm, after measured COD in culture medium, ammonia nitrogen, under total phosphorus index
Half drops, to obtain two generation pregnant solutions；Then two generation pregnant solutions is taken to be added in enriched medium according to the ratio of 1:10,
25-35 DEG C, continue enrichment culture under 140rpm, until COD in culture medium after measured, ammonia nitrogen, total phosphorus index drop by half are so far rich
Collection finishes to obtain enrichment flora.
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| CN109467181A (en) * | 2019-01-09 | 2019-03-15 | 安徽霖德生物科技有限公司 | A kind of biology needle film water processing system and method for treating water |
| CN109576201A (en) * | 2019-01-31 | 2019-04-05 | 上海同瑞环保科技有限公司 | A method of culture indigenous microorganism efficiently removes water body ammonia nitrogen |
| CN110591998A (en) * | 2019-08-30 | 2019-12-20 | 中国热带农业科学院橡胶研究所 | Screening and domesticating method of natural rubber deodorization flora |
| CN111268807A (en) * | 2020-01-21 | 2020-06-12 | 武汉工程大学 | Method for removing ammonia nitrogen in residual ammonium salt leachate of rare earth leaching site by using indigenous denitrification microbial flora |
| CN111268857A (en) * | 2020-01-21 | 2020-06-12 | 武汉工程大学 | Method for removing high-concentration ammonia nitrogen in residual ammonium salt leachate of rare earth leaching site by chemical-biological method |
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| CN114525236A (en) * | 2020-11-23 | 2022-05-24 | 天津大学 | River sediment microbial activation composite microbial agent and preparation method and application thereof |
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| CN109467181B (en) * | 2019-01-09 | 2023-11-14 | 安徽霖德科技有限公司 | Biological needle membrane water treatment system and water treatment method |
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| CN110591998A (en) * | 2019-08-30 | 2019-12-20 | 中国热带农业科学院橡胶研究所 | Screening and domesticating method of natural rubber deodorization flora |
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| CN111268857A (en) * | 2020-01-21 | 2020-06-12 | 武汉工程大学 | Method for removing high-concentration ammonia nitrogen in residual ammonium salt leachate of rare earth leaching site by chemical-biological method |
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